Changes In Flux Research Articles

Initial Solidification Behavior of Spring Steel Billet Near the Mold Corner During Continuous Casting Process

Strand surface defects originate from the initial solidification of molten steel in continuous casting mold. This article investigates the initial solidification behavior during the continuous casting of 55SiCr spring steel billet using a novel mold simulator. First, the high‐frequency responding temperatures and high‐frequency heat fluxes across the mold corner and hot face are calculated by the 2D‐inverse heat conduction program mathematical model and the fast Fourier transformation, and the results indicate that the temperature variations and heat flux fluctuations around mold corner are stronger than those at mold hot face. Then, the characteristic heat fluxes around the meniscus at mold corner and hot face are analyzed, and their four corresponding signals are discussed with the help of power spectral density analysis. The average solidification factor K for shell corner is greater than shell face, which indicates that the cooling intensity of shell at corner is stronger due to the two‐dimensional heat transfer near mold corner, as demonstrated by heat transfer and dendrite structure analysis. Finally, the interrelations between the shell profile, mold oscillation, heat flux change rate, high‐frequency heat flux, temperature change rate, and high‐frequency temperature for the two cases are discussed.

Effect of Carbon on Void Nucleation in Iron.

The study reports the significance of carbon presence in affecting void nucleation in Fe. Without carbon, void nucleation rates decrease gradually at high temperatures but remain significantly high and almost saturated at low temperatures. With carbon present, even at 1 atomic parts per million, void nucleation rates show a low-temperature cutoff. With higher carbon levels, the nucleation temperature window becomes narrower, the maximum nucleation rate becomes lower, and the temperature of maximum void nucleation shifts to a higher temperature. Fundamentally, this is caused by the change in effective vacancy diffusivity due to the formation of carbon-vacancy complexes. The high sensitivity of void nucleation to carbon comes from the high sensitivity of void nucleation to the vacancy arrival rate in a void. The void nucleation is calculated by first obtaining the effective vacancy diffusivity considering the carbon effect, then calculating the defect concentration and defect flux change considering both carbon effects and pre-existing dislocations, and finally calculating the void nucleation rate based on the recently corrected homogeneous void nucleation theory. The study is important not only in the fundamental understanding of impurity effects in ion/neutron irradiation but also in alloy engineering for judiciously introducing impurities to increase swelling resistance, as well as in the development of simulation and modeling methodologies applicable to other metals.

Progressive inhibition of [formula omitted] reaction in wood char packed bed by in-situ evolving [formula omitted]: Experimental insights and theoretical analysis

Previous research on Char reactions with gas phase compounds under micro-thermogravimetry systems shows that hydrogen inhibits heterogeneous char reactions. However, its impact on larger gasification systems with evolving hydrogen profiles remains largely unexplored. This study examines a macro-scale wood char bed to understand the influence of in situ evolving hydrogen on char reactions. When subjected to a specific steam flux, carbon conversion and pore morphology changes are mainly confined to the bed’s upstream, with the downstream char retaining its original characteristics. Numerical investigations reveal over 75 % of species production and consumption occurs within the initial 20 % of bed height. Fourier-transform infrared spectroscopy confirms hydrogen-induced inhibition in downstream segments, showing a shift from C–OH to C–H bonds. Particle-scale analysis indicates significantly higher rates of hydrogen diffusion and adsorption compared to H2O, impeding downstream C+H2O reactions. Increased temperature, higher reactant concentrations, or reduced residence time can overcome this inhibition, enhancing conversion rates. These findings are critical for optimizing steam-to-biomass ratios in oxy-steam gasification systems for generating hydrogen-rich syngas.

A novel magnet system—which enables uniformity of radial flux density by means of a parabolic yoke—applied in the Planck-Balance

Abstract A uniform magnetic flux density that is effective in the movement range of the coil is essential for accurate Kibble balance experiments. By utilizing Hopkinson’s law and Kirchhoff’s circuit laws, basic formulas have been derived, providing a method to calculate the magnetic flux density distribution in the airgap of a cylindrical magnet system. A parabolic outer contour of the inner yoke has been found to be a suitable solution to achieve uniformity. Experiments show, that this approach results in a relative change of magnetic flux density in the order of 3 ⋅ 10 − 4 per 8 mm movement range, using a magnet system with a mass of only 2.3 kg. Therefore, the system will be integrated into the upgraded version of PTB’s Planck-Balance—a compact variant of a Kibble balance—aiming for sub 1 ⋅ 10 − 6 accuracy level determination of the geometric factor. The solution described provides a comparatively easy means to design a cylindrical magnet system, using only one permanent magnet disc, without the use of complex simulation software.

A Numerical Study of Tropical Cyclone and Ocean Responses to Air‐Sea Momentum Flux at High Winds

AbstractThe relationship between minimum sea level pressure (MSLP) and maximum wind speed, is a commonly employed metric for assessing tropical cyclone (TC) forecast skill. However, accurately reproducing this relationship in TC forecasts is challenging. By introducing a new air‐sea momentum flux scheme considering both wave state and saturation (decrease) effect at high winds into a fully coupled atmosphere‐ocean‐wave model, our numerical results reveal that the maximum wind speed and MSLP respond oppositely to the air‐sea momentum flux change. The simulated wind‐pressure relationship aligns well with observations when the new flux scheme is used. This study highlights the large sensitivity of wind‐pressure relationship to the air‐sea momentum flux parameterization at high winds. Furthermore, the air‐sea momentum flux has a significant effect on ocean wave characteristics and sea surface temperature (SST) cooling in TC simulations.

The joint effects of the El Niño-Southern Oscillation and Arctic Oscillation on tropical Indian Ocean heat flux during boreal winter

In this study, the joint effects of the El Niño-Southern Oscillation (ENSO) and Arctic Oscillation (AO) on winter net surface heat flux (Qnet) anomalies over the tropical Indian Ocean (TIO) are investigated for the 1979/1980–2017/2018 period. The results show that, in multisource datasets, the Qnet pattern for El Niño plus positive AO cases is the most robust. The major feature of Qnet is dominated by a significant dipole pattern, with oceanic heat gain anomalies appearing over the southeastern TIO and oceanic heat loss near the western TIO. Analysis of the flux components shows that the Qnet anomalies are mainly contributed by changes in latent heat flux and shortwave radiation (QLHF and QSWR), which are tightly associated with the regional atmospheric and oceanic conditions. In association with positive AO, northerly wind anomalies on the eastern flanks of a low-level anomalous anticyclone in the Arabian Sea enhance the intertropical convergence zone (ITCZ) in the western TIO. Meanwhile, the El Niño-related SST warming occurs in the western TIO. Both enhanced ITCZ and anomalous SST warming favour in situ increased low and middle cloud cover and a reduced QSWR. Furthermore, under El Niño's effect, a low-level anomalous anticyclone located in the southeastern TIO leads to a positive QLHF through decreased near-surface wind speed and increased near-surface air humidity. As a result, a dipole Qnet pattern tends to be observed for the combination of El Niño and positive AO.

Source Region and Launch Characteristics of Magnetic-arch-blowout Solar Coronal Mass Ejections Driven by Homologous Compact-flare Blowout Jets

We study the formation of four coronal mass ejections (CMEs) originating from homologous blowout jets. All of the blowout jets originated from NOAA Active Region (AR) 11515 on 2012 July 2, within a time interval of ≈14 hr. All of the CMEs were wide (angular widths ≈ 95°–150°), and propagated with speeds ranging between ≈300 and 500 km s−1 in LASCO coronagraph images. Observations at various EUV wavelengths in Solar Dynamics Observatory/Atmospheric Imaging Assembly images reveal that in all the cases, the source region of the jets lies at the boundary of the leading part of AR 11515 that hosts a small filament before each event. Coronal magnetic field modeling based on nonlinear force-free extrapolations indicates that in each case, the filament is contained inside of a magnetic flux rope that remains constrained by overlying compact loops. The southern footpoint of each filament is rooted in the negative polarity region where the eruption onsets occur. This negative polarity region undergoes continuous flux changes, including emergence and cancellation with opposite polarity in the vicinity of the flux rope, and the EUV images reveal brightening episodes near the filament’s southeastern footpoint before each eruption. Therefore, these flux changes are likely the cause of the subsequent eruptions. These four homologous eruptions originate near adjacent feet of two large-scale loop systems connecting from that positive polarity part of the AR to two remote negative polarity regions, and result in large-scale consequences in the solar corona.

Bayesian neuromorphic imaging for single-photon LiDAR.

This paper proposes a Bayesian approach to enable single photon avalanche diode (SPAD) arrays to be used as pseudo event cameras that report changes in the scene. Motivated by the working principle of event cameras, which produce sparse events associated with light flux changes, we adopt a changepoint detection strategy to generate intensity and depth change event streams from direct time-of-flight (dToF) sequences measured by SPAD arrays. Although not our main goal, the algorithm also produces as a by-product, intensity and depth estimates. Unlike the output of passive event cameras that only correspond to light flux changes, the change events detected from the sequential dToFs can relate to changes in light flux and/or depth. The integration of the proposed Bayesian approach with single-photon LiDAR (SPL) systems provides a novel solution to achieve active neuromorphic 3D imaging that offers the advantages of significantly reduced output redundancy and in particular the capacity to report scene depth changes. For each pixel of the SPAD array, asynchronous events are generated by performing online Bayesian inference to detect changepoints and estimate the model parameters simultaneously from individual single-photon measurements. Experiments are conducted on synthetic data and real dToF measurements acquired by a 172×126 pixel SPAD camera to demonstrate the feasibility and efficiency of the proposed Bayesian approach.

Impacts of Atlantic and Pacific Multidecadal Variability on South American Precipitation and Temperature in the CESM Simulations and Observations

AbstractThe Community Earth System Model version 1 (CESM1) and version 2 (CESM2)'s abilities to simulate the impacts of Atlantic multidecadal variability (AMV) and Pacific multidecadal variability (PMV) on South American precipitation and temperature have not been assessed, and how the AMV and PMV modulate each other's influences on South American climate is not well understood. Here we use observations, reanalyses, and CESM1 and CESM2 simulations from 1920 to 2015 to study those problems. The models can reproduce the observed precipitation and temperature responses to AMV well, but can only roughly reproduce such responses to PMV. The precipitation response over the South Atlantic convergence zone (SACZ) is better simulated by CESM2 compared to CESM1, which is associated with an improved horizontal moisture flux over this region. However, the models cannot accurately simulate the observed differences between the influences of Pacific interannual and multidecadal variability on South American precipitation and temperature. The impacts of AMV and PMV on South American precipitation are modulated by the other mode via changes in horizontal moisture flux over the SACZ and River Plate basin in summer, as well as changes in vertical motion over the equatorial regions in winter. Similarly, the impacts of AMV and PMV on South American temperature are also modulated by the other mode. Over water‐limited regions, such as northeastern Brazil and southern Argentina, the precipitation and temperature responses are anti‐correlated, possibly via surface evaporation.

Strengthened impact of late autumn Arctic sea ice on Asian winter cold extremes after 1999/2000

Winter cold extremes (WCEs) frequently plague densely populated areas of Asia, leading to substantial economic losses and even fatalities. It has been found that the late autumn sea ice concentration (SIC) anomalies in the northern (SICN) and southern Arctic (SICS) are significantly positively and negatively correlated with the occurrence frequency of WCE in Asia, respectively (Wang and Su 2024). Our study demonstrates that the impacts of SICN and SICS have strengthened after 1999/2000. Specifically, before 1999/2000, the influences of SICN and SICS on the Asian WCE (AWCE) were relatively weak, possibly related to the weak intensity of SICS and the limited correlation between SICN and SICS. After 1999/2000, the interannual variability of SICS became larger and anti-correlated with that of SICN, resulting in a stronger teleconnection between the Arctic SIC and AWCE. It is revealed that after 1999/2000, the greater loss of SICS modified atmospheric stability through changes in surface heat fluxes and surface upward longwave radiation fluxes. This alteration weakened the magnitudes of westerly winds and increased the frequency of blocking events over the northern Eurasian continent, leading directly to a higher occurrence of cold extremes in Asia. These interdecadal differences in the influence of Arctic SIC on AWCE may be associated with long-term climate change.

Carbon fluxes and water-use efficiency in a Pinus tabuliformis plantation in Northeast China and their relationship to drought

The impact of extreme weather events on carbon fluxes and water-use efficiency (WUE) in revegetated areas under water-limited conditions is poorly understood. We analyzed changes in carbon fluxes and WUE over three years of eddy-covariance measurements in a Pinus tabuliformis plantation in Northeast China to investigate carbon fluxes and WUE responses to drought events at different time scales. Mean annual net ecosystem exchange (NEE), gross primary production (GPP), and ecosystem respiration (Re) were −368.48, 1042.42, and 673.94 g C m−2, respectively. Drought events increased NEE, as GPP was more sensitive to water stress than Re at different growing stages. Mean annual WUE was 2.46 g C kg−1 H2O, and plant phenology played a key role in WUE responses to drought. Water stress had negative and positive effects on daily WUE at the early and late growing stages, respectively, and daily WUE was generally insensitive to drought at the mid growing stage. A lagged effect existed in the carbon fluxes and WUE dynamics after drought events at various time scales. Water stress at the early growing stage was more important than that at other growing stages on annual carbon sequestration and WUE, as it dominated canopy growth in the current year. The annual mean normalized difference vegetation index controlled interannual variations in carbon fluxes and WUE in the plantation. Our findings contribute to the prediction of possible changes in carbon and water fluxes under climate warming in the afforested areas of Northeast China.

NAcM-OPT protects keratinocytes from H2O2-induced cell damage by promoting autophagy.

This study aimed to investigate the protective effect of NAcM-OPT, a small molecule inhibitor of defective in cullin neddylation 1 (DCN1), on H2O2-induced oxidative damage in keratinocytes. Immortalized human keratinocytes (HaCaT cells) were treated with NAcM-OPT and exposed to oxidative stress. CCK-8 assays were used to measure cell viability. The mGFP-RFP-LC3 dual fluorescent autophagy indicator system was utilized to evaluate changes in autophagic flux. Western blotting was used to measure the expression of the autophagy-related proteins LC3 and Beclin 1. Keratinocytes were treated with the autophagy activator rapamycin, and HaCaT cell supernatant was added to PIG1 cells (immortalized human melanocytes), followed by evaluation of tyrosinase (TYR) expression via qRT-PCR. NAcM-OPT increased cell viability and cell proliferation. Furthermore, this molecule promoted autophagic flux through increased expression of autophagy-related proteins under H2O2-induced oxidative stress. Additionally, rapamycin increased the mRNA levels of TYR in PIG1 cells. Moreover, NAcM-OPT alleviated mitochondrial damage, restored mitochondrial function, and upregulated the expression of NFE2L2, HO1, NQO1, and GCLM. Importantly, NAcM-OPT also increased epidermal thickness, follicle length, and melanin synthesis under oxidative stress in vivo. These findings suggest that NAcM-OPT may be a promising small molecule antioxidant drug for the treatment of vitiligo.

Nitrogen addition and precipitation reduction alter ecosystem multifunctionality and decrease soil nematode abundance and trophic energy fluxes in a temperate forest

Soil biota and energy flow within their food webs have a profound impact on various ecosystem functions. However, there are still significant gaps in our understanding of how climate change factors, such as nitrogen deposition and reduced precipitation, impact ecosystem functioning, particularly in temperate forest ecosystems. In this study, we evaluated the response of ecosystems to long-term nitrogen deposition and/or reduced precipitation due to climate change. We assessed these responses by examining nematode community energy fluxes and their associated ecosystem multifunctionality. Field experiments were conducted in a temperate forest to simulate nitrogen deposition (5.0 kg N·ha−1·yr−1) using NH4NO3 application and to reduce precipitation (to 70 % of a normal year) using rain shelters. Our findings showed that nitrogen addition and/or precipitation reduction treatments altered soil nematode composition. Nitrogen addition alone reduced the abundance and energy fluxes of high trophic groups such as omnivore-predators, which served as proxies for total abundance and energy fluxes. In contrast, precipitation reduction did not exhibit significant effects on overall abundance and energy fluxes. Environmental factors influencing changes in soil nematode abundance and energy fluxes included soil pH, NH4+-N, and total nitrogen in the soil. Nitrogen addition increased ecosystem multifunctionality by promoting nutrient cycling, while precipitation reduction promoted plant productivity and inhibited carbon cycling, without changing ecosystem multifunctionality. Regression analysis showed that soil nematode energy fluxes were negatively correlated with ecosystem multifunctionality. The effects of these two factors on soil nematode food webs showed temporal variation, as well as indicators related to multiple functions of the ecosystem. Our study suggests that soil nematode energy fluxes are more sensitive than nematode abundance for quantifying the relationship between biodiversity and ecosystem multifunctionality.

ATP-dependent citrate lyase Drives Left Ventricular Dysfunction by Metabolic Remodeling of the Heart.

Metabolic remodeling is a hallmark of the failing heart. Oncometabolic stress during cancer increases the activity and abundance of the ATP-dependent citrate lyase (ACL, Acly ), which promotes histone acetylation and cardiac adaptation. ACL is critical for the de novo synthesis of lipids, but how these metabolic alterations contribute to cardiac structural and functional changes remains unclear. We utilized human heart tissue samples from healthy donor hearts and patients with hypertrophic cardiomyopathy. Further, we used CRISPR/Cas9 gene editing to inactivate Acly in cardiomyocytes of MyH6-Cas9 mice. In vivo, positron emission tomography and ex vivo stable isotope tracer labeling were used to quantify metabolic flux changes in response to the loss of ACL. We conducted a multi-omics analysis using RNA-sequencing and mass spectrometry-based metabolomics and proteomics. Experimental data were integrated into computational modeling using the metabolic network CardioNet to identify significantly dysregulated metabolic processes at a systems level. Here, we show that in mice, ACL drives metabolic adaptation in the heart to sustain contractile function, histone acetylation, and lipid modulation. Notably, we show that loss of ACL increases glucose oxidation while maintaining fatty acid oxidation. Ex vivo isotope tracing experiments revealed a reduced efflux of glucose-derived citrate from the mitochondria into the cytosol, confirming that citrate is required for reductive metabolism in the heart. We demonstrate that YAP inactivation facilitates ACL deficiency. Computational flux analysis and integrative multi-omics analysis indicate that loss of ACL induces alternative isocitrate dehydrogenase 1 flux to compensate. This study mechanistically delineates how cardiac metabolism compensates for suppressed citrate metabolism in response to ACL loss and uncovers metabolic vulnerabilities in the heart.

Altered glucose kinetics occurs with aging: a new outlook on metabolic flexibility.

Our purpose was to determine how age affects metabolic flexibility and underlying glucose kinetics in healthy young and older adults. Therefore, glucose and lactate tracers along with pulmonary gas exchange data were used to determine glucose kinetics and respiratory exchange ratios [RER = carbon dioxide production (V̇co2)/oxygen consumption (V̇o2)] during a 2-h 75-g oral glucose tolerance test (OGTT). After an 12-h overnight fast, 28 participants, 15 young (21-35 yr; 7 men and 8 women) and 13 older (60-80 yr; 7 men and 6 women), received venous primed-continuous infusions of [6,6-2H]glucose and [3-13C]lactate with a [Formula: see text] bolus. After a 90-min metabolic stabilization and tracer equilibration period, volunteers underwent an OGTT. Arterialized glucose concentrations ([glucose]) started to rise 15 min post glucose consumption, peaked at 60 min, and remained elevated. As assessed by rates of appearance (Ra) and disposal (Rd) and metabolic clearance rate (MCR), glucose kinetics were suppressed in older compared to young individuals. As well, unlike in young individuals, fractional gluconeogenesis (fGNG) remained elevated in the older population after the oral glucose challenge. Finally, there were no differences in 12-h fasting baseline or peak RER values following an oral glucose challenge in older compared to young men and women, making RER an incomplete measure of metabolic flexibility in the volunteers we evaluated. Our study revealed that glucose kinetics are significantly altered in a healthy aged population after a glucose challenge. Furthermore, those physiological deficits are not detected from changes in RER during an OGTT.NEW & NOTEWORTHY To determine metabolic flexibility in response to an OGTT, we studied healthy young and older men and women to determine glucose kinetics and changes in RER. Compared to young subjects, glucose kinetics were suppressed in older healthy individuals during an OGTT. Surprisingly, the age-related changes in glucose flux were not reflected in RER measurements; thus, RER measurements do not give a complete view of metabolic flexibility in healthy individuals.

Changes in the tropical upper-tropospheric zonal momentum balance due to global warming

Abstract. We study the zonal momentum budget of the deep upper tropics in the context of present and future climates. In the zonal mean, as is known, a robust balance exists between the acceleration by the horizontal eddy momentum flux convergence and the deceleration by the mean meridional momentum advection. During summer, climatological stationary Rossby waves over the Asian monsoon longitudes converge westerly momentum into the tropics and are the primary contributors to the eddy term. During winter, anomalous westerly winds over the tropical east Pacific allow extratropical waves to propagate into the deep tropics, where they tend to break and decelerate the flow. When integrated over all longitudes, we find that eddies from these two regions sum constructively in summer and destructively in winter, always yielding a net positive momentum forcing that balances the mean flow term. The state-of-the-art CMIP6 suite qualitatively captures these features in the historical run and shows that the momentum fluxes change due to global warming. In summer, stationary eddy circulations in the Asian monsoon zone weaken in the upper troposphere (UT) but strengthen in the lower stratosphere (LS). Greater upward mass flux from the troposphere forces a stronger divergence and a more intense circulation in the LS following a Sverdrup vorticity balance. We observe this strengthening of summertime tropical and subtropical stationary waves in the LS over all longitudes and verify it in an idealized aquaplanet general circulation model experiment. In winter, we find that eddy westerlies over the east Pacific longitudes decrease in strength due to the expected weakening of the subtropical stationary waves with warming. This causes a significant decrease in the propagation of extratropical waves into this region, along with a drop in eddy potential vorticity fluxes associated with these waves. Thus, apart from the mean meridional flux, which weakens due to the projected weakening of the Hadley cells, our analysis of warming simulations clearly suggests significant and robust changes in the eddy momentum fluxes in the deep tropics. Potential implications of these changes in the context of the zonal mean flow and regional circulations are discussed.

Thermal property characterization and modeling of SMAW electrode coating flux using ANN and regression analysis

The design and development of SMAW electrode coating fluxes, along with their thermal property characterization, are discussed. Using an extreme vertices design approach, 27 flux mixture combinations have been prepared. The TGA-DSC and hot disk analysis are done to quantify the thermal response of the fluxes. Results show that increase in CaF2 and SiO2 reduces weight loss. Increase in CaO increases weight loss, whereas increase in BaO shows minimal effect on weight loss. Enthalpy change of flux was found to influence mainly with CaF2 and CaO. Thermally stable flux has lower volatile compounds such as crystallization water and moisture, which helps to minimize gaseous entrapment in weld pool. An increase in CaF2 shows slight decreases and then increase in thermal diffusivity. The increase in thermal diffusivity leads to instant heat flow in the coating. Regression analysis is done, and the effect of individual and interaction of flux constituents on thermal properties is discussed. The artificial neural network is employed for predictive modeling in the domain of the mixture design approach, its prediction accuracy is compared with regression analysis. Water vapor solubility of slag was estimated between 3.169 and 3.947 and sulfide capacity of slag was − 2.479 to −1.892.

The Inter‐Model Uncertainty of Projected Precipitation Change in Northern China: The Modulating Role of North Atlantic Sea Surface Temperature

AbstractPrecipitation changes in northern China are projected to increase in the Coupled Model Inter‐comparison Project Phase 6 (CMIP6) multi‐model ensemble. However, these projections are accompanied by notable inter‐model uncertainty, and the sources of this uncertainty remain largely unexplored. By analyzing 30 CMIP6 models, this research explores the source of inter‐model uncertainty in projected precipitation change and reveals the fundamental mechanism driving uncertainty spread. Following the empirical orthogonal function of inter‐model projected precipitation change, the leading mode displays a seesaw spatial pattern between northwest and north China. This phenomenon predominantly stems from the inter‐model divergence of projected sea surface temperature (SST) warming in the North Atlantic. Further scrutinizing the ocean mixed layer heat budget, we discover that the combined effect of surface sensible heat flux, net surface shortwave radiation flux, and ocean heat transport convergence influences heat flux and SST change of North Atlantic. The multi‐model projections indicate that localized increases in solar radiation and heat convergence warm sea surface, raising SST and initiating convective motion. This convective motion subsequently transforms the 200 hPa teleconnection wave train, leading to an anti‐phase pattern over northern China. This wave pattern modulates total cloud cover percentage, influences surface upward latent heat flux, and adjusts the top of atmosphere outgoing longwave radiation, collectively resulting in the seesaw pattern. Our study underscores the pivotal role of inter‐model disparities in North Atlantic SST warming projection, which is a primary driver of precipitation uncertainty in northern China. These insights offer an essential foundation for refining and diminishing inter‐model uncertainty.

Seasonal variation in greenhouse gas concentrations and diffusive fluxes in three river–reservoir systems in the Seine Basin (France)

River and reservoir ecosystems have been considered as hot spots for GHG (greenhouse gas) emissions while their specific hydrological and biogeochemical processes affect GHG concentrations; however, few studies integrated river–reservoir systems to identify the dominant drivers of GHG concentrations and flux changes associated with these systems. In the present study, we examined the seasonal variations in GHG concentrations in the surface water of three river-reservoir systems in the Seine Basin. The levels and seasonal variations of GHG concentrations exhibited distinct patterns among reservoirs, upstream, and downstream rivers. The concentrations of CH4 (methane) in the reservoirs were notably higher than those observed in both upstream and downstream rivers and showed higher values in summer and autumn, which contrasted with CO2 (carbon dioxide) concentrations, while N2O (nitrous oxide) concentrations did not show an obvious seasonal pattern. A high mole ratio of CH4/CO2 was found in these reservoirs, with a value of 0.03 and was more than 30 and 10 times higher than that in the upstream and downstream rivers, respectively. The three river–reservoir systems were oversaturated with GHG during the study period, with the average diffusive fluxes (expressed as CO2eq: CO2 equivalent) of 810 ± 1098 mg CO2eq m−2 d−1, 9920 ± 2413 mg CO2eq m−2 d−1, and 7065 ± 2704 mg CO2eq m−2 d−1 in the reservoirs, upstream and downstream rivers, respectively. CO2 and CH4–CO2 were respectively the dominant contributors to GHG diffusive fluxes in river and reservoir sections, while N2O contributed negligibly to GHG diffusive fluxes in the three river–reservoir systems. Our results showed that GHG concentrations and gas transfer coefficient have varying importance in driving GHG diffusive fluxes among different sections of the river–reservoir systems. In addition, our results also show the combined effect of reservoirs and upstream rivers on the water quality variables and hydrological characteristics of downstream rivers, highlighting the future need for additional investigations of GHG processes in the river–reservoir systems.

Changes in protein fluxes in skeletal muscle during sequential stages of muscle regeneration after acute injury in male mice

Changes in protein turnover play an important role in dynamic physiological processes, including skeletal muscle regeneration, which occurs as an essential part of tissue repair after injury. The inability of muscle tissue to recapitulate this regenerative process can lead to the manifestation of clinical symptoms in various musculoskeletal diseases, including muscular dystrophies and pathological atrophy. Here, we employed a workflow that couples deuterated water (2H2O) administration with mass spectrometry (MS) to systematically measure in-vivo protein turnover rates across the muscle proteome in 8-week-old male C57BL6/J mice. We compared the turnover kinetics of over 100 proteins in response to cardiotoxin (CTX) induced muscle damage and regeneration at unique sequential stages along the regeneration timeline. This analysis is compared to gene expression data from mRNA-sequencing (mRNA-seq) from the same tissue. The data reveals quantitative protein flux signatures in response to necrotic damage, in addition to sequential differences in cell proliferation, energy metabolism, and contractile gene expression. Interestingly, the mRNA changes correlated poorly with changes in protein synthesis rates, consistent with post-transcriptional control mechanisms. In summary, the experiments described here reveal the signatures and timing of protein flux changes during skeletal muscle regeneration, as well as the inability of mRNA expression measurements to reveal changes in directly measured protein turnover rates. The results of this work described here provide a better understanding of the muscle regeneration process and could help to identify potential biomarkers or therapeutic targets.