Epilobium Angustifolium Research Articles

Phytochemical Insights and Therapeutic Potential of Chamaenerion angustifolium and Chamaenerion latifolium.

The Chamaenerion genus, particularly Chamaenerion angustifolium and Chamaenerion latifolium, is recognized for its rich phytochemical composition and extensive medicinal properties. These species are abundant in polyphenols, flavonoids, and tannins, which contribute to their potent antioxidant, antimicrobial, and anticancer activities. This review provides a comprehensive analysis of their phytochemical constituents, with an emphasis on how processing methods, including fermentation, influence bioactivity. Notably, fermentation enhances the levels of key bioactive compounds, such as oenothein B, gallic acid, and ellagic acid, thereby increasing their pharmacological potential. Additionally, this review evaluates the biological activities of Chamaenerion species in relation to their chemical composition, while also considering the limitations of current studies, such as the lack of in vivo or clinical trials. The literature for this review was sourced from scientific databases, including PubMed, Scopus, and ScienceDirect, covering research from 2010 to 2024. Future studies should focus on optimizing extraction methods, elucidating synergistic bioactivities, and conducting in-depth clinical trials to validate their efficacy and safety.

Exploring the Phytochemical Profile and Biological Insights of Epilobium angustifolium L. Herb.

The aerial parts of Epilobium angustifolium L. (fireweed) (Onagraceae) are renowned for their use in the treatment of prostatic, kidney and urinary tract diseases, and skin infections. In this work, a comprehensive phytochemical profiling of the methanol-aqueous extract from E. anfustifolium aerial parts was performed by the means of liquid chromatography-Orbitrap high-resolution mass spectrometry. Annotation and dereplication of 121 secondary metabolites were achieved, including acylquinic acids, gallo- and ellagitannins, flavonoids, phenolic acids, and their glycosides. Forty-six compounds are reported for the first time in the species. Total phenolic and flavonoid content were 85.04 ± 0.18 mg GAE/g and 27.71 ± 0.74 mg QE/g, respectively. Antioxidant capacity assessment revealed that the extract actively scavenged DPPH and ABTS radicals (310.74 and 466.82 mg TE/g) along with a high reducing power in CUPRAC and FRAP assay (442.83 and 291.50 mg TE/g), respectively, and metal chelating (48.20 mg EDTA/g). The extract also had a distinct impact on α-glucosidase (3.48 mmol ACAE/g) and moderate activity towards α-amylase (0.44 mmol ACAE/g) and lipase (8.03 OE/g). It inhibited acetyl- and butyrylcholinesterase (2.05 and 1.67 mg GALE/g) and had a prominent anti-tyrosinase effect (61.91 mg KA/g). Our results contribute to establishing fireweed as a multifunctional agent for use in herbal preparations.

Extraction of Bioactive Compounds and Influence of Storage Conditions of Raw Material Chamaenerion angustifolium (L.) Holub Using Different Strategies.

The study evaluates different preparation methods for identifying the best strategy for extracting biologically active compounds from raw Chamaenerion angustifolium (L.) Holub plant material. The methodologies include direct aqueous methanol extraction with a combination of natural aerobic and anaerobic fermentation for 24-72 h, followed by 35 °C and 60 °C drying. Furthermore, the study also focuses on determining the different temperature storage conditions on the stability of biologically active compounds. UV-VIS spectroscopy was used to quantitatively evaluate the total content of phenolic compounds, flavonoids, and radical scavenging activity. For qualitative analysis, chromatographic separation with electrochemical detection (ED) of extracted compounds, a gradient high-performance liquid chromatography (HPLC) system was used. Study results indicate that 48 h natural aerobic fermentation followed by 35 °C drying and 75% (v/v) aqueous methanol extraction yielded the maximum amount of biologically active compounds in Chamaenerion angustifolium (L.) Holub leaves, blossom, and stem samples. Freezing samples in liquid nitrogen had the lowest impact on the total content of phenolic compounds, flavonoids, and radical scavenging activity. HPLC-ED system results identified chlorogenic acid, oenothein B, trans-p-Coumaric acid, ellagic acid, and rutin in Chamaenerion angustifolium (L.) Holub leave samples.

A Review of the Anti-Inflammatory Skincare Potential of Epilobium angustifolium (Fireweed) Inspired by Herbal Tradition

The demand for naturally derived cosmetic ingredients is rising as consumers increasingly prioritize green and environmentally friendly products. Naturally derived ingredients, such as plant extracts rich in polyphenols like flavonoids and tannins, are often perceived as more sustainable and beneficial for skincare. Epilobium angustifolium (fireweed) is a circumpolar plant with traditional uses in Northern and Eastern Europe known for its skincare benefits. This review explores the chemical composition, traditional uses, and potential skincare benefits of E. angustifolium, focusing on its anti-inflammatory and antioxidant properties. Literature surveys, ethnopharmacological research, and database searches were conducted to investigate the properties of E. angustifolium. The plant contains various compounds such as flavonoids, phenolic acids, tannins, steroids, and triterpenes, each with potential skincare benefits. Pharmacological reports suggest that E. angustifolium exhibits antioxidant and antibacterial properties, making it a valuable ingredient in skincare products. The availability of E. angustifolium in the market as a raw material for cosmetics offers opportunities for formulators to create innovative products with skin-conditioning and anti-inflammatory properties. Understanding the chemical composition and bioactivity of E. angustifolium can guide the development of effective and sustainable skincare formulations.

Recreational Assessment of Green Belt Forests of the Ulaanbaatar City

The classification of forest vegetation of the Tuul river basin within the green belt of the Ulaanbaatar city. Two associations (Linnaeo borealis–Pinetum sibiricae and Vaccinio vitis-idaeae–Laricetum sibiricae) were classified Vaccinio–Piceetea. Diagnostic signs and characteristics of the ecological and geographical peculiarities of the units were presented, anthropogenic transformation was assessed. To determine the models of succession processes, the series of permanent sample plots were established in the communities of the Linnaeo borealis–Pinetum sibiricae association, located at different distances from the city, in taxation units with unequal intensity of recreational load. Recreational forest assessment was calculated according to the approach by V.S. Romanov and L.N. Rozhkov (1974). In the Linnaeo borealis–Pinetum sibiricae association, large areas were occupied by moderately transformed communities. The Vaccinio vitis-idaeae–Laricetum sibiricae association is characterized by being weakly transformed forests. Different stages of anthropogenic pressure were formed serial communities, unequal in structure and species diversity. Serial plant communities of allogeneic succession were developed according to 2 models of geitogenesis — tolerance (Linnaeo borealis–Pinetum sibiricae ↔ Rhytidium rugosum; Linnaeo borealis-Pinetum sibiricae ↔ Chamaenerion angustifolium) and inhibition (Linnaeo borealis–Pinetum sibiricae ↔ Betula rotundifolia). A comprehensive assessment of the recreational potential was based on three indicators: natural conditions, air hygiene and landscaping. All activities aimed at increasing the recreational potential should contribute to the improvement of these indicators. A prerequisite for increasing the recreational forest assessment should be functional zoning of the territory with the allocation of infrastructure, walking and sanitary protective zones. Forest management is of great importance in increasing the recreational potential of forests. The forest management project for the organization of recreational forests should be aimed at the formation of sustainable and aesthetically attractive forest plantations.

Flavonoids, Phenolic Acids, and Tannin Quantities and Their Antioxidant Activity in Fermented Fireweed Leaves Grown in Different Systems.

The increasing demand for organic and biodynamically cultivated fireweeds worldwide has led to a paucity of studies on the effects of solid-phase fermentation and various growth techniques on the quantities of biologically active substances and their antioxidant activity. This experiment was carried out in 2023 at the organic farm in the Jonava district (Safarkos village, Lithuania). The aim of this work was to investigate polyphenols (flavonoids and phenolic acids) and antioxidant activity in fireweed (Chamerion angustifolium (L.) Holub) leaves fermented for 24 and 48 h in solid-phase fermentation and natural, organic, and biodynamic cultivation. Fireweeds have high quantities of polyphenols and strong antioxidant activity. The method employed for determining antioxidant activity was spectrophotometric, for measuring polyphenols, high-performance liquid chromatography (HPLC). Principal component analysis (PCA) was used to determine the relationships between the average content of total polyphenols and antioxidant activity in fermented fireweed leaves grown in different systems. This study's findings demonstrated that the leaves of fireweed cultivated organically had the greatest concentration of total flavonoids, total phenolic acids, and total polyphenols. Comparing the fermentation process effect, the amount of predominant phenolic acids-chlorogenic, p-coumaric, and ellagic acids-as well as the content of oenothein B, during the fermentation process significantly decreased, but the concentration of quercetin-3-O-glucoside after a short time of the fermentation process significantly increased. According to the obtained results, it would be possible to create various health-giving and nature-friendly products from fireweeds.

Elemental Analysis of Five Medicinal Plants Species Growing in North Ossetia Using Neutron Activation Analysis

One important category of traditional remedies is medicinal plants, which are widely consumed by the population and often subjected to contamination. For the first time, the elemental composition of five wild medicinal plants traditionally consumed in the Republic North Ossetia—brook-mint (Mentha longifolia), oregano (Origanum vulgare), St. John’s wort (Hypеricum perforаtum), thyme (Thymus daghestanicus), and fireweed (Chamaenerion angustifolium)—and corresponding soil samples was determined. This investigation aimed to assess the degree of toxic element accumulation in plants and the possible toxic effect of the analyzed plants on human health. The analysis encompassed the quantification of 36 major and minor elements in soil samples, alongside the detection of 23 elements in plant samples using instrumental neutron activation analysis. According to contamination and enrichment factors, which were calculated in order to assess the level of soil pollution, elements such as As, Sb, Zn, and U in soil may originate from both geogenic and anthropogenic sources. In all plant samples, the most abundant major elements were K and Ca and their content ranged from 9870 to 49,500 mg·kg−1 and 5400 to 20,000 mg·kg−1, respectively, while among the microelements, Fe (54–2080 mg·kg−1) and Mn (27.8–190 mg·kg−1) can be highlighted as the most abundant. The transfer factor was calculated to estimate metal uptake from soil in plants. The highest values of the transfer factor were obtained for Mo, K, Ca, and Zn. The daily intake of metals and the health risk index were calculated to assess the safety of the collected plants. The health risk index was below the threshold for all plants suggesting a probable non-carcinogenic effect. Principal component analysis and linear discriminant analysis were used as classification techniques. The principal component analysis allowed us to define the main groups of elements and associate them with their sources of origin, while discriminant analysis enabled us to discriminate plant samples by species.

Variety of active ingredients in leaves and flowers of <i>Chamaenerion angustifolium</i> (L.) Scop., depending on type of natural population

Relevance. Nowadays the popularity of Chamaenerion angustifolium as a medicinal plant and as a tea is growing noticeably. Fireweed is an eclogically plastic species, that does not require high soil fertility and is widespread in Russia. Medical and food industries use leafs as a row material of fireweed. Series of phytochemical studies of Chamaenerion raw material have shown that it contains a wide variety of pharmacologically significant compounds, that accumulate as a result of both primary and secondary metabolism. The most important groups, formed as a result of secondary metabolism, are polyphenols, including flavonoids, phenolic acids and ellagitannins (including enothein B). Concentration of these chemical compounds equals 15% and even hither of the raw material (leaf).The aim. Identification of variability and relationships between the content of various groups of phenolic compounds in raw material of Chamaenerion angustifolium, depending on ecological conditions.Methods. At the first stage of our work was used the cartographic method. Were studied specimens from 27 points, growing in ecologically different areas of the Moscow, Kaluga and Yaroslavl regions. Phenolic compounds were determined using a spectrophotometer: flavonoids in terms of rutin, tannins and the amount of phenolic compounds, using the Folin-Ciocalteu reagent in terms of gallic acid.Results. The content of polyphenols, tannins and flavonoids in raw material of fireweed, collected in different ecological and soil aspects, was measured. Measurement results were compared, using correlation analysis. As the result of content analysis, it was found that the content of polyphenols in the leaves ranged from 9 to 11%, the content of flavonoids fell within the chain range of 2.5-3.5%, tannin content – 5.0-6.5%. A close correlation was found between the content of phenolic compounds and tannins (R=0.972). It indicates, that the shikimate pathway is the main process of biosynthesis secondary metabolites in the Chamaenerion angustifolium plant. Also it means the leading role of local ecological conditions of the studied areas.

A Botanical Tour in the Rajmahal Hills of Bihar

The paper presents the main features of geology, topography, climate and vegetation met with in Rajmahal hills and enumerates 364 species of angiosperms, 13 species of Pteridophytes, and 16 species of mushrooms collected during a short tour undertaken in December, 1957. An analysis of different plant communities characterising various habitats and of the floristics and distribution of species collected brings up 9 species viz. Arthraxon castratus (Griff.) Narayanaswami, Bidens biternata (Lour.) Merr. & Sherff., Carpesium cernuum Linn., Cyathula prostrata Bl., Echinocarpus murex Benth., Epilobium angustifolium Linn., Evolvulus nummularius Linn., Hygrophila salicifolia T. And., Inula eupatorioides DC. as new records of species and Cyathula as new record of genus for Bihar. A new combination, viz. Embelia tsjeriam-cottam (R. & S.) A. DC. var.ferruginea (Wall.) Panigrahi comb. nov. is proposed.

POTENCJAŁ ZASTOSOWANIA FITOZWIĄZKÓW Z DZIKOROSNĄCYCH ROŚLIN ZIELNYCH W PREWENCJI I TERAPII WYBRANYCH CHORÓB CYWILIZACYJNYCH

The prevalence of individual civilisational diseases has been constantly increasing in recent years. Their occurrence is related, among other factors, to changes in lifestyle (stress, low levels of physical activity, low-quality diet), increasing urbanisation and industrialisation (environmental pollution). Lifestyle- dependent diseases include type 2 diabetes and Alzheimer's disease which is not only related to lifestyle - one of the main risk factors is age. The increasing life expectancy of the population associated with the development of civilisation (health, social and welfare) therefore has a significant impact on its occurrence. In 2017, the number of people with diabetes was approximately 476 million, which represented an increase of 129.7% compared to 1990 [1]. For Alzheimer's disease, incidence and prevalence increased by 147.95% and 160.84% respectively between 1990 and 2019 [2]. The economic and social costs associated with the occurrence of these diseases are enormous [3,4]. Among the drugs used in the treatment of Alzheimer's disease, compounds of natural origin that have the cholinesterase inhibitor activity - the isoquinoline alkaloid galantamine - have been successfully used [101], as well as the semi-synthetic phisostigmine derivative isolated from Physostigma venenosum – rivastigmine (Fig. 4.) [5,6]. One of the most commonly used drugs in the treatment of diabetes is metformin, a synthetic derivative of galegin isolated from Galega officinalis (Fig. 2.) [7]. Natural-derived compounds can therefore be highly active and safe preparations used in both the prevention and treatment of certain diseases. The present work aims to review and summarise information on the potential use of herbaceous wild plants: Stellaria media, Epilobium angustifolium and Chenopodium album in the prevention and therapy of diabetes and Alzheimer's disease. Promising results have been obtained in both in vitro and in vivo studies on the potential use of extracts from these plants. They exhibited protective (neuroprotective activity, protection of organs from damage in the progress of diabetes, effects on body weight control and obesity reduction) and therapeutic (effects on lowering blood glucose levels, reducing insulin resistance, inhibitory effects on cholinesterase, α-glucosidase and α-amylase) activities. Polyphenolic compounds and flavonoids were shown to be important in determining the biological activity of the extracts. Available literature data indicate a high potential for the use of extracts obtained from these plants, both in the prevention and therapy of type 2 diabetes and Alzheimer's disease.

Impact of ash fallout of the 2023 Shiveluch volcano eruption (Kamchatka) on forest vegetation

The ash fallout of Shiveluch volcano (April 11, 2023, Kamchatka) is the most intensive volcanic ash fallout of the last 60 years. We present the results of the reconnaissance fi eld investigations supplemented with analyses of satellite images of the area for estimations of deposits distribution and area aff ected by the ash fallout. We also estimated the properties of fi ne grained ash of andesitic composition and its infl uence on the forest vegetation composed by Picea yezoensis (Siebold et Zucc.) Carrière, Larix cajanderi Mayr и Betula ermanii Cham. As a result of moderate- scale ash fallout in the forest (thickness of freshly deposited ash is 5–8 cm), the negative impact on vegetation was minimal because the ash was fi ne-grained and was deposited on a thick snowpack, and the leaves were not formed yet. The forest vegetation on lower elevations was not damaged at all but strong changes occurred in grass and bush vegetation. We have observed the diminished density of plant coverage, decreased size of grass vegetation and a decreased number of plant species. Calamagrostis purpurea (Trin.) Link. s. l., a broadly represented dominant species among grass vegetation, became less common, but Chamerion angustifolium (L.) Holub became more widespread. The possibility of small plants to grow through the ash layer turned out to be strongly reduced. Mosses and lichens in the forest have been buried under the ash, the recovery could take several decades. We have found a notable impact on plants by ash remobilized by strong wind, this impact is the strongest in the open areas and on forest edges. This process can continue for many years due to the large volume of the deposited ash covering the open spaces of the volcano foot.

The genome sequence of rosebay willowherb Chamaenerion angustifolium (L.) Scop., 1771 (syn. Epilobium angustifolium L., 1753) (Onagraceae).

We present a genome assembly from an individual Chamaenerion angustifolium (fireweed; Tracheophyta; Magnoliopsida; Myrtales; Onagraceae). The genome sequence is 655.9 megabases in span. Most of the assembly is scaffolded into 18 chromosomal pseudomolecules. The mitochondrial and plastid genome assemblies have lengths of 495.18 kilobases and 160.41 kilobases in length, respectively.

The very early-succession herbaceous vegetation in the ‘Vaia’ windstorm clearings within the Italian southeastern pre-Alpine mountain belt (Veneto and Trentino)

Abstract The very early herbaceous vegetation which established in the clearings following 2018 ‘Vaia’ storm was investigated in some pre-Alpine areas of Northeast Italy, on calcareous as well as acidic substrata. Sixty-two original vegetation-plot records were executed in spruce or mixed beech-silver fir-spruce blowdown forests, within two years after the salvage logging had been completed. According to different origin and degree of soil disturbance, different communities were recognised. Galeopsis pubescens and G. tetrahit rich stands develop as ephemeral annual associations at the beginning of the regeneration succession where partially decomposed coniferous needles and twigs have accumulated in the litter. Soils with altered profiles due to forestry machineries harbour dominance of perennial herbaceous species (especially Senecio nemorensis agg., Atropa bella-donna, Epilobium angustifolium) which origin as many already recognised associations or vegetation types we ascribed to community level. All coenoses belong to Epilobietea angustifolii class, with the exception of Calamagrostis arundinacea-rich stands on undisturbed base-rich as well as base-poor soils, whose syntaxonomic positions are unclear.

Rosebay Willowherb (Chamerion angustifolium) in Food Products: Evaluation of the Residual Anti-radical Activity of Polyphenol Compounds and N-acetylcystein

Background: The composition of polyphenolic compounds in various parts of the Rosebay willowherb (Chamerion angustifolium) has been studied in detail and published. A number of these compounds have sufficient antiradical activity (ARA) to create functional foods based on extracts from this plant that are preventive against free-radical pathologies in humans. Part of substances with antiradical activity (e.g., vitamins, polyphenol components) are destroyed during the technological processing of food raw materials, and the values of ARA drop in the finished food product concerning the initial values (before and after technical processing) are not fully evaluated. Objective: The object of the study is to study the thermal stability of polyphenol components possessing ARA (dried at 60°C and 150°C) and evaluate their residual ARA in the finished food products, N-acetylcysteine (NAC), as one of the known antiradical, was used as a reference substance. Methods: Spectrophotometric method was used to evaluate the change of ARA in prepared foods. Gas chromatography-mass spectrometry (GC-MS) was used as an additional method to evaluate the difference in the chemical composition of polyphenol components. Results: The residual ARA in the finished products (for caramel and bakery products) was ≈ 23.9% for rapidly extractable substances, for polyphenol compounds ≈ 46.1%, and for amino acids and easily hydrolyzable substances ≈ 34.5%. Conclusion: Rosebay willowherb extract and N-acetylcysteine are recommended for increasing the ARA (between 1.6-4.9 times) of foods with low ARA (confectionery, bread, or other heavily processed foods) and for diets and diets preventive of free-radical pathologies by adding polyphenolic plant components and cysteine derivatives.

Self-powered high-performance UV–vis–IR hybrid photovoltaic photodetector based on Epilobium angustifolium L. (Onagraceae)

Self-powered high-performance UV–vis–IR hybrid photovoltaic photodetector based on Epilobium angustifolium L. (Onagraceae)

Antioxidant activities of traditional medicinal plant species from the Kars (Turkey)

Fourteen different plant parts consisting of leaves, roots, flowers, and seeds were examined separately of Rumex patientia, Hyoscyamus niger, Melilotus officinalis, Lamium album, and Epilobium angustifolium plants. All of these are naturally growing and used for medicinal purposes in Kars province. Five different methods were used to determine the antioxidant capacity of these herbal products. The methods were as follows: determination of total polyphenol, total flavonoid, DPPH● (free radical), CUPRAC (cupric reducing antioxidant capacity), and FRAP (ferric ıon reducing antioxidant power). The data obtained regarding the antioxidant activity demonstrated that the flower part of the Epilobium angustifolium plant obtained from Kars had the highest antioxidant capacity. It was determined that the seeds of the Hyoscyamus niger plant had the lowest antioxidant capacity.

Gamete sex and elevation affect genetically based variation for unreduced gamete production in a mixed-ploidy plant.

Unreduced gametes are the primary mechanism of neopolyploid formation. Their production in diploid populations is arguably maladaptive, but the magnitude and patterns of genetically based variation maintained in natural populations are poorly understood. We examined variation in male and female unreduced gamete production among plants from different elevations in fireweed, Chamerion angustifolium, grown in a common environment. Using seeds from three high-elevation and three low-elevation diploid populations in one study, and a single diploid population in another, we estimated realized rates of unreduced male (sperm) and female (egg) gamete production by reciprocally pollinating diploid and tetraploid plants and estimating the incidence of tetraploid seeds using flow cytometry. Unreduced gamete frequencies per plant were similar in the two studies (0.12% vs. 0.08%). High-elevation populations had a greater percentage of fruit with seeds from unreduced gametes, but a lower percentage of seeds per fruit than low-elevation populations. Female unreduced gamete frequencies differed among elevations, but male frequencies did not, and the gamete sexes were not correlated at the plant level. We conclude that genetically based variation for unreduced gametes is maintained within and among natural populations, despite their fitness disadvantages, suggesting that local selection may be ineffective at purging them under some conditions.

Development of the root system of Poa pratensis L. influenced by phytomass of competitive plants

The periodic surface application of phytomass of Solidago canadensis L., Filipendula ulmaria (L.) Maxim, Chamaenerion angustifolium (L.) and Scop., and Cirsium setosum (Willd.) Besser ex M.Bieb., results in variations in the development of underground organs of Poa pratensis, including the stimulation or inhibition of rhizome formation. The noted effects are associated with variations in the structure and properties of soils, similar to those observed for green manure (siderate plants).

Assessment of raw material reserves of promising medicinal plants in the territory of Western Altai in Eastern Kazakhstan

The results of resource studies of economically valuable species of medicinal plants of the Western Altai (East Kazakhstan region) growing on the territory of 7 forestry municipal state institutions (FMSI) are presented: Zyryanovsk, Ridder, Pikhtovskoye, Cheremshanskoye, Malubinskoye, Verkh-Ubinskoye and Ust-Kamenogorsk. Western Altai occupies the right bank of the Irtysh, includes the interfluve of the Uba, Bukhtarma and Narym and is represented by a system of mountain ranges in the northern part of the Kazakhstan Altai. The operational reserves and volumes of possible procurement of raw materials of 8 species of medicinal plants were taken into account. For specific thickets of sites or model samples, raw material reserves were recorded. Traditional geobotanical methods were used to describe plant communities involving resource objects. For four species, the presence of commercial thickets with a supply of air-dried raw materials was established. from 300 to 2000 tons: Veratrum lobelianum, Sanguisorba officinalis, Filipendula ulmaria, Epilobium angustifolium. For other species, raw material reserves varied from 1 to 65 tons: Aconitum leucostomum, Inula helenium, Achillea millefolium, Bupleurum multinerve. The raw material base of the identified species of medicinal plants in the territory East Kazakhstan region is able to meet the needs of the domestic pharmaceutical industry.

Estimation of efficiency of ultrasonic extraction using deep eutectic solvents for extraction of biologically active compounds from plant raw materials

Deep eutectic solvents are novel media for the extraction of biologically active substances from plant row material. They are often used for ultrasonic extraction. However, due to the high viscosity of the deep eutectic solvents, the question of the sufficiency of ultrasonic exposure for effective diffusion of the target components into the solution remains open. The purpose of this study was to compare the effectiveness of ultrasonic extraction with and without additional mixing. The antioxidant activity of Chamaenerion angustifolium (L.) Scop. extracts obtained by these two methods in the medium of two deep eutectic solvents were compared. It has been shown that additional mixing increases the extraction efficiency concerning antioxidant activity by 20-80%. The obtained data can be used for the development of modern technologies for the isolation of biologically active compounds from plant material using deep eutectic solvents.