Cerebral Blood Flow In Hippocampus Research Articles

Glutamate pharmacology and the treatment of schizophrenia: current status and future directions

Glutamate-containing neuronal terminals are ubiquitous in the central nervous system and their functional importance in mental activity is considerable. Therefore, the involvement of this neurotransmitter in the pathology of schizophrenia is being studied. Biochemical evidence has suggested that glutamatergic transmission may be regionally reduced in schizophrenia, although this evidence has never been completely consistent nor fully replicable. More striking has been the behavioral effects in humans of the antiglutamatergic drugs phencyclidine (PCP) and its congener ketamine. By historical report, PCP produces a 'schizophrenia-like' psychosis in normal humans and aggravates the psychosis in schizophrenics. More recently, ketamine has been shown to produce a mild psychotomimetic effect in normal volunteers, which has some schizophrenia-like features. We have studied the effects of ketamine in schizophrenic patients. Here, ketamine intensified each patient's specific underlying psychosis, an effect not blocked by haloperidol. Moreover, ketamine selectively increased cerebral blood flow (CBF) in the anterior cingulate cortex and reduced CBF in hippocampus and lingual gyrus. These data may be pertinent to the subject's psychosis exacerbation, especially because both cingulate and hippocampus have been previously implicated in schizophrenic psychosis. In addition, ketamine produced a distinctive dynamic time-course of regional CBF changes in different anatomic regions, with immediate (5-10 min) changes in cingulate, but somewhat more delayed changes (20-40 min) in the thalamus and cerebellum. Our immediate early gene (IEG) time-course data with c-fos and zif268 in rats following PCP suggest that a single dose of this antiglutamatergic compound can have an effect in some brain areas which lasts beyond 48 h, an effect which is distinct by IEG and by region. Together, these data suggest that glutamate-mediated neurotransmission has a strong influence in schizophrenia, although the specifics of this involvement have yet to be articulated.

Somatosensory regulation of regional hippocampal blood flow in anesthetized rats.

The effect of noxious or non-noxious mechanical stimulation of various cutaneous areas on cerebral blood flow in hippocampus was examined with laser Doppler flowmetry in urethane-anesthetized artificially-ventilated rats. Noxious mechanical stimulation (pinching) of the skin on the face, forepaw, chest, or hindpaw for 20s increased regional hippocampal blood flow (Hpc-BF) and systemic blood pressure, but non-noxious mechanical stimulation (brushing) had no such effect. After the spinal cord was transected at T1 level a forepaw pinch caused no change in blood pressure but still increased Hpc-BF. This suggests that cutaneous noxious stimulation can induce pressor-independent increases in Hpc-BF. The increase in Hpc-BF induced by a forepaw pinch in T1-transected rats was partially reduced by intravenous administration of mecamylamine (2 mg/kg), a nicotinic cholinergic receptor antagonist. Atropine (0.5 mg/kg), a muscarinic cholinergic antagonist was ineffective. These data indicate that the cholinergic vasodilative system is involved in the somatically-induced increase in Hpc-BF via activation of the nicotinic cholinergic receptors.

Age-related vulnerability to cerebral ischemia in spontaneously hypertensive rats.

We sought to determine the effects of aging on regional cerebral blood flow and ischemic brain damage in transient cerebral ischemia in rats. Five adult (5-6 months) and five aged (18-22 months) female spontaneously hypertensive rats were subjected to 20 minutes of bilateral carotid occlusion and 60 minutes of recirculation under amobarbital anesthesia (100 mg/kg i.p.). Regional cerebral blood flow in the hippocampus and striatum was measured using the hydrogen clearance method. Nine adult and 14 aged rats were subjected to 20 minutes of bilateral carotid occlusion or were sham-operated under ether anesthesia. Seven days after 20 minutes of cerebral ischemia, the rats' brains were perfusion fixed. Ischemic damage in the hippocampus and striatum was graded (0 [normal] to 3 [majority of neurons damaged]). After 20 minutes of bilateral carotid occlusion, striatal cerebral blood flow decreased to 9.1 +/- 1.5 and 3.9 +/- 2.0 ml/100 g/min in aged and adult rats, respectively, and hippocampal cerebral blood flow decreased to 8.6 +/- 2.4 and 5.7 +/- 2.4 in aged and adult rats, respectively. Although these ischemic cerebral blood flow values were not significantly different between the two age groups, scores for ischemic damage in the hippocampus CA-1 subfield and striatum were significantly higher in aged than in adult rats (p less than 0.05, Kruskal-Wallis' h test with Bonferroni correction). We conclude that aging may be a primary factor in the development of greater ischemic neuronal damage observed in aged hypertensive rats.

Diurnal variation of cerebral blood flow in rat hippocampus.

We measured local cerebral blood flow over 24 hours in 10 unanesthetized, freely moving rats to determine whether blood flow in the hippocampus fluctuated as a function of time of day. We measured hydrogen clearance at 1-hour intervals using a polyurethane-coated platinum electrode with a 1-mm bare tip implanted in the dorsal hippocampus. Individual rats displayed a wide range of local cerebral blood flow values (from 30 to 100 ml/min/100 g tissue) in a day. In seven of the 10 rats, the overall mean hippocampal blood flow for the dark cycle (7 PM-5 AM) was significantly (p less than 0.001, 0.01, or 0.05) greater than that for the light cycle (6 AM-6 PM), showing an average increase of 20%. Further, the maximum mean hippocampal blood flow at 11 PM in all 10 rats was 42% greater than the minimum at noon. Our study demonstrates for the first time that local cerebral blood flow in the hippocampus shows diurnal variation.

Stimulation of the septal complex increases local cerebral blood flow in the hippocampus in anesthetized rats

The effects of focal stimulation, either electrically or chemically, of the septal complex (i.e. the medial septal nucleus and the nucleus of the diagonal band) on the local hippocampal cerebral blood flow (Hpc CBF) measured by laser Doppler flowmetry were examined in anesthetized rats. Electrical stimulation of the septal complex produced a current-dependent increase in Hpc CBF that was accompanied by an increase in extracellular acetylcholine (ACh) release in the hippocampus. Microinjection of L-glutamate (50–100 nmol) into the septal complex also produced an increase in Hpc CBF. The l-glutamate-induced vasodilative response of Hpc CBF was markedly attenuated after administration of nicotinic cholinergic blocking agent, mecamylamine (2 mg/kg, i.v.). It was suggested that the cholinergic septohippocampal nerve fibers act as an intracerebral vasodilative neural system by releasing ACh from the nerve terminals in the hippocampus and by activating the nicotinic cholinergic receptor.

Efficacy and mechanism of action of a calcium channel blocker after global cerebral ischemia in rats.

Dihydropyridine calcium channel blockers such as nicardipine are under evaluation for treating acute cerebral ischemia because they may increase cerebral blood flow by causing vasodilation and because they may be cytoprotective in part by limiting production of arachidonic acid metabolites. We demonstrated in a previous study that nicardipine improves postischemic neuronal function, as measured by somatosensory evoked potentials, without reducing the extent of light-microscopic CA-1 hippocampal histologic damage. To characterize further the effect of nicardipine on global ischemic injury, we administered the drug beginning 24 hours before 30 minutes of four-vessel ischemia in Wistar rats. We then measured hippocampal ATP, phosphocreatine, and glucose contents immediately and 2 hours after ischemia, and measured learning ability (working and reference errors) on an eight-arm radial maze beginning 30 days after ischemia. To gain insight into the possible mechanism of action, we measured production of arachidonic acid metabolites (eicosanoids: TXB2 and 6-keto-PGF1 alpha) and hemispheric and hippocampal cerebral blood flow by the [14C]butanol indicator fractionation technique immediately and 2 hours after ischemia. Nicardipine was associated with fewer working errors (p less than 0.02) but no difference in reference errors. The drug had no effect on energy metabolites, cerebral blood flow, or eicosanoids immediately after ischemia, but ATP, phosphocreatine, and cerebral blood flow all returned to normal levels significantly more rapidly during reperfusion in treated rats. Nicardipine improves behavioral, electrophysiologic, and mitochondrial function after ischemia without preventing cellular damage and improves postischemic reperfusion. The drug's positive effect appears to occur during reperfusion.