Potato mop-top virus and its protist vector, Spongospora subterranea, cause internal and external damage to potato tubers that make them unmarketable. Currently there are no effective control methods to eliminate these soilborne and seedborne pathogens, so resistant germplasm is highly desired. A greenhouse assay was designed and validated to screen a large number of plants grown in both sandy soil and potting mix. High pathogen pressure was selected (20 sporosori/gram of soil) to ensure successful inoculation, with an incubation time of 60-90 days. The successful use of conetainers was demonstrated to screen a large number of plants in limited greenhouse space. Thirteen susceptible commercial cultivars or breeding lines were screened in the greenhouse, with S. subterranea detected in 99.5% of the plants grown in both soil types. Potato mop-top virus was detected in 79.4% of the plants grown in five-inch clay pots filled with sandy soil and 97.5% of the plants screened in conetainers filled with potting mix. The high pathogen levels detected in the 13 susceptible cultivars indicate that it is possible to assess potato germplasm resistance to S. subterranea and potato mop-top virus in the greenhouse. In the absence of a research field with consistently high pressure of S. subterranea and potato mop-top virus, this new greenhouse assay could rapidly identify germplasm with resistance to these two economically important pathogens year-round. Future identification of S. subterranea or potato mop-top virus resistant germplasm will enable development of disease resistance markers that can help breeders rapidly identify resistant material.
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