Central Composite Design Method Research Articles

A Quality by Design (QbD) Based Method Development and Validation of a High-Performance Liquid Chromatography for the Simultaneous Estimation of Metformin and Ertugliflozin

This study explains about the Analytical Quality by Design approach for the optimization of a High-Performance Liquid Chromatography Method for the simultaneous estimation of Metformin and Ertugliflozin in pharmaceutical substance. The study aimed to optimize the High-Performance Liquid Chromatography (HPLC) by means of an analytical target profile in order to achieve good separation of compounds along with acceptable analysis time. Identification of risk factors for variables affects the method efficacy. This leads to the development of an accurate, precise, and economic method. The optimized conditions of the developed method were a stationary phase of a Discovery C18 250 x 4.6mm, 5m and a mobile phase of Orthophosphoric acid buffer (pH 2.2),ACN taken in the ratio 60:40 was selected as mobile phase and detection wavelength of 230nm. The flow rate was selected as 0.98ml/min at 29.150C column temperature. Using the central composite design (CCD) method was optimized. The method is showing the linearity over the concentration range of 25-150µg/ml for Metformin and 0.375-2.25µg/ml for Ertugliflozin. The intra-and inter-day precision were less than 2% of relative standard deviation. Accuracies between 99-102% of the true values.The LOD obtained for Metformin and Ertugliflozin were found to be 59 and 3.7, respectively. LOQ obtained for Metformin and Ertugliflozin were 77.6 and 5.2, respectively.Under accelerated conditionsdegradation percentage of the drug was found to be less than 10%, and the degradation product peak not affecting the system suitability of Metformin and Ertugliflozin.

Central Composite Design Optimisation in Single Point Incremental Forming of Truncated Cones from Commercially Pure Titanium Grade 2 Sheet Metals.

Single point incremental forming (SPIF) is an emerging process that is well-known to be suited for fabrication in small series production. The aim of this paper was to determine the optimal input parameters of the process in order to minimise the maximum of both the axial and the in-plane components of the forming force achieved during SPIF and the surface roughness of the internal surface of truncated-cone drawpieces. Grade 2 pure titanium sheets with a thickness of 0.4 mm were used as the test material. The central composite design and response surface method was used to determine the number of experiments required to study the responses through building a second-order quadratic model. Two directions of rotation of the forming tool were also considered. The input parameters were spindle speed, tool feed rate, and step size. The mathematical relations were defined using the response surfaces to predict the surface roughness of the drawpieces and the components of the forming force. It was found that feed rate has an insignificant role in both axial and in-plane forming forces, but step size is a major factor affecting axial and radial forming forces. However, step size directly affects the surface roughness on the inner surfaces of the drawpieces. Overall, the spindle speed −579 rpm (clockwise direction), tool feed 2000 mm/min, and step size 0.5 mm assure a minimisation of both force components and the surface roughness of drawpieces.

Effect of thermal debinding and sintering parameters on the mechanical properties of 4605 MIM compact using the RSM

ABSTRACT In metal injection moulding (MIM) process, improper debinding or sintering process is mainly responsible for forming defects in final parts. In this study, the effects of thermal debinding and sintering parameters on the physical and mechanical properties of low alloy steel parts produced by the MIM process are investigated. For this reason, the design of experiments was done using the central composite design (CCD) method with parameters of 3–7 °C/min for heating rate, 1–3 h for sintering time, and 1200–1300 °C for temperature. The density, strength, hardness, and elongation of the sintered parts were measured for each experiment. The results showed that among the process variables, the heating rate has the most significant effect on the properties mentioned above. Also, the impact of sintering temperature showed to be more significant than the sintering time. Finally, it was observed that with the increase in the thermal debinding rate from 3 to 7 °C/min, the relative density decreased by 3%, and elongation decreased from 10 to 4%. As for the strength, there is an optimum point of heating rate after which, with the increase of heating rate (from 5 to 7 °C/min), the strength decreases significantly by 70 MPa.

Optimization of the Electrospun Niobium-Tungsten Oxide Nanofibers Diameter Using Response Surface Methodology.

The present research aimed to investigate the effect of working parameters on the electrospinning of niobium–tungsten oxide nanofibers and optimize the process using central composite design (CCD) based on the response surface methodology (RSM). An experiment was designed to assess the effects of five variables including the applied voltage (V), spinning distance (D), polymer concentration (P), flow rate (F), and addition of NaCl (N) on the resulting diameter of the nanofibers. Meanwhile, a second-order prediction model of nanofibers diameter was fitted and verified using analysis of variance (ANOVA). The results show that the diameter of the nanofibers was significantly influenced by all the variables except the flow rate. Some second-order and cross factor interactions such as VD, DP, PF, PN, and P2 also have significant effects on the diameter of the nanofibers. The results of the ANOVA yielded R2 and adjusted R2 values of 0.96 and 0.93 respectively, this affirmed that the predictive model fitted well with the experimental data. Furthermore, the process parameters were optimized using the CCD method and a maximum desirability function of 226 nm was achieved for the diameter of the nanofibers. This is very close to the 233 nm diameter obtained from a confirmatory experiment using the optimum conditions. Therefore, the model is representative of the process, and it could be used for future studies for the reduction of the diameter of electrospun nanofibers.

Optimization of one-step production of biodiesel by Sulfuric acid/Stannic oxide-Hangjin2 clay catalyzed xanthoceras sorbifolia bunge kernel

ABSTRACT In this study, biodiesel has been successfully prepared from Xanthoceras sorbifolia seed kernel by SO4 2-/SnO2-Hangjin2#clay as catalyst. Based on the Central Composite Design method, the effect of the various parameters influencing the biodiesel process have been studied and optimized. The maximum yield of biodiesel (90.67%w/w) can be obtained when the mass fraction of the catalyst is 0.7%, the mass ratio of methanol to oil is 4:1 V/m, the methanol content in the oil is 12%, and the reaction temperature achieves 81°C。Besides, the consequence of Infrared spectroscopy and Gas chromatography can assure the production of biodiesel from the xanthoceras sorbifolia bunge oil. Based on these results, it can be concluded that Xanthoceras sorbifolia bunge seed oil is an acceptable non-edible feedstock for biodiesel production.

Optimization of operating parameters of seeding device in plot drill with seeding control system

Plot drill has a significant impact on field breeding by replacing manual seeding. However, the seeding performance of plot drill needs to be further improved as the mechanical transmission method cannot work under the optimal combination of operating parameters. In this study, a cone compartment tray of sowing device was evaluated under the laboratory conditions, using a self-designed seeding control system to adjust the operating parameters. Among them, a plot seeding control system was involved that could set operating parameters through an Android terminal, which effectively reduced the difficulty of parameters adjustment. The method of central composite experiment design was employed to conduct experiments and explore the effects of experiment factors such as lifting height of storage tube (LHST), rotation speed of cone compartment tray (RSCCT), and rotation speed seed distributor (RSSD) on the seeding uniformity coefficient of variation among rows (SUCVR) and the rate of damaged seeds (RDS). For SUCVR of wheat, the results showed that the importance order of main factors from high to low was RSSD, RSCCT and LHST. And for SUCVR of buckwheat, RSCCT had the largest influence, followed by RSSD, and LHST had the smallest influence. For RDS of wheat and buckwheat, only RSSD had a very significant effect among the main factors. The experiments data of wheat indicated that when operated at 27.2 mm for LHST, 4.5 r/min for RSCCT and 1169 r/min for RSSD, the sowing performance of wheat was optimal, with SUCVR and RDS values of 5.02% and 0.117%, respectively. The buckwheat seeding performance was found to be optimal when the LHST, RSCCT and RSSD were 26.5 mm, 3.9 r/min and 711 r/min, and SUCVR and RDS were 4.74% and 0.113%. The seeding control system realized the electromechanical control of the seeding equipment, was convenient for parameter setting and stepless adjustment, and could be operated under an optimized combination of operating parameters. The research results could provide a reference for the performance improvement and application of plot drill. Keywords: plot drill, seeding device, seeding control system, seeding performance, parameter optimization DOI: 10.25165/j.ijabe.20211403.6218 Citation: Cheng X P, Li H W, He J, Wang Q J, Lu C Y, Wang Y B, et al. Optimization of operating parameters of seeding device in plot drill with seeding control system. Int J Agric & Biol Eng, 2021; 14(3): 83–91.

COD Reduction in Petrochemical Wastewater Using the Solar Photo-Fenton Process

Water has been recognized as the most fundamental factor in organisms' lives and the most widely used element in industries, while currently, the world is dealing with water scarcity in many areas. This emphasizes the importance of preventing water contamination as well as returning contaminated water produced by industries to the production and consumption cycle. Yet, the need for environmental protection is a certain principle that is generalized in today's world. This necessity has become more important with the growth of industries and technologies and subsequent contamination. Advanced oxidation technology has been substantially developed in recent decades, becoming increasingly important in the treatment process of industrial wastewaters containing resistant organic materials that cannot be removed through conventional treatment methods to reduce water quality parameters. The present study has examined the chemical oxygen demand (COD) in the synthetic monoethyl amine wastewater prepared by the solar Photo-Fenton process. Principal effective parameters in the advanced oxidation technology, including the processing time, the concentration of hydrogen peroxide ion, the concentration of iron (II) ion, and pH, were investigated by the response surface methodology (RSM) through 30 random experiments using central composite design method (CCD) to optimize reaction conditions. The most sufficient operational conditions were achieved at pH=4, [Fe2+] =2 mM, [H2O2] =20 mM, and t=90 min for the COD removal rate of 77.08%.

Aplikasi Disain Komposit Pusat Pada Proses Pengecatan Mobil Bekas

The business of buying and selling used cars is increasingly substantial in Indonesia, in the era of globalization progressively developing depends on demands a process that must be fast, precise, and economical. Repair to body, chassis, and engine are competency expertise in the field of Automotive Engineering that emphasizes automotive repair service skills. at now selling price of used cars, it is necessary to do repairs, the process of repairing used cars or better known as refurbishment work pre-owned cars is mostly done by small-scale used car buying and selling businesses. In order for used cars to return to their like-new condition, generally small-scale used car sellers carry out the process of repairing the vehicle themselves, especially repairs to the vehicle's body paint. This study aims to process of painting a Toyota Avanza car bodypaint against the thickness of the paint using a spray booth tool by using the model of central composite design (CCD). The effect of spray-on booth temperature, interstice size of the spray gun, and time according to the thickness of the paint will be analyzed using the CCD method. This spray booth painting technology is expected to help reduce bodypaint lead time. The results of the ANOVA (Analysis of Variance) regression analysis, the temperature of the spray booth, interstice size of the spray gun, and time are the factors that most significantly affect the thickness of the paint. The operating conditions to produce optimal paint thickness are temperature 55oC, interstice size of spray gun of 1.7 mm and time of painting and drying of 30 minutes, the resulted of a thickness of the paint for used and new cars are 130.2 μm and 81.84 μm., with a coefficient of determination for used and new cars of 90.78% and 96.19%.

Optimization of Preparation Conditions for Nano Porous Catalytic Carbon From Post Consumer Poly Ethylene Terphthalate Bottles Using Response Surface Methodology

Using response surface techniques, this research proposes the best conditions for producing Nano porous catalytic carbon from post-consumer polyethylene terephthalate (PET). The carbon yield and surface area of post-consumer PET-based AC is evaluated. The PET-based AC was rendered using a zinc chloride-based chemical activation technique (ZnCl2). The results of three preparation variables, including activation temperature, activation time, and chemical impregnation (ZnCl2:char) ratio, on both surface area and yield, were studied. To assess the impact of planning conditions on responses, the central composite design (CCD) method was used Quadratic models for surface area and yield were constructed using the CCD. The study of variance revealed the effects of the three main parameters, the properties of activated carbon, and the important factors on each experimental design reaction (ANOVA). Using a 5% impregnation ratio, a 1000°C activation temperature, and the best conditions for PET-based AC preparation were found to be at 60-minute activation time, yielding 1102.62 m2/g of surface area and a 16.218 % yield.

Optimization of Ni(II), Pb(II), and Zn(II) Ion Adsorption Conditions on Pliocene Clays from Post-Mining Waste

Lignite mining results in a significant amount of waste clayey materials—the so-called Pliocene clays, which are often considered useless mineral waste. One method of management is to use them as inexpensive adsorbents in water and wastewater treatment, or as permeable barriers in groundwater remediation. The study is an attempt to use the raw Pliocene clays to remove Pb(II), Ni(II), and Zn(II) ions from wastewaters. The mineralogical composition, physicochemical, granulometric, structural, and surface properties were examined. The value of the point of zero charge pH of the adsorbent was found to be 7.1. To determine the optimal adsorption conditions, the central composite design (CCD) method was used with the pH of the solution, metal ion concentration, and the adsorbent mass as independent variables, and the percentage removal as the response variable. The determined optimal values of the adsorbent mass and the solution pH turned out to be similar for all three metals, with a pH of 6–7 and an adsorbent dose of 18–24 g/dm3. In further tests, the pH was established slightly lower than it would appear from the CCD method in order to avoid the possible precipitation of metals in the form of hydroxides. The kinetics of sorption were investigated and it was found that the sorption process ran for several minutes. The effect of temperature showed that the adsorption was spontaneous and thermodynamically favored. The leachability of the metals indicated that Pb(II) and Zn(II) were strongly bound in the adsorbent, whereas Ni(II) manifested a larger mobility. It was found that the tested clays had quite good sorption properties in relation to the tested metal ions and could be used for water and wastewater treatment.

Benzothiazole derivative-functionalized carbon dots for the detection of berberine hydrochloride

Berberine hydrochloride (BRH) has a wide range of biomedical values, including antioxidant, antibacterial and anti-cancer, so it is very important to establish a rapid and effective method for the determination of BRH. Herein, the fluorescent CDs with rich carboxyl groups on the surface were synthesized by the facile one-pot hydrothermal treatment of citric acid and methionine. The surface of CDs was further modified with 2-(4-aminophenyl)benzothiazole (ABT) to obtain CDs-ABT. The emission generated by the surface energy trap becomes more stable after surface modification, so CDs-ABT (31%) has a larger quantum yield than CDs (25%). In addition, BRH can significantly quench the fluorescence of CDs-ABT through inner filter effect (IFE). Experiments were carried out by using central composite design (CCD) and response surface method (RSM) and the best conditions for detecting BRH were obtained. Excellent linear correlation between the BRH concentration in the range of 10–130 μM and the fluorescence quenching of CDs-ABT was observed, and the limit of detection down to 3.5 nM. The assay was successfully applied to the detection of BRH in actual drug samples.

Preparation an Optimal Hydrogel of Water Shutoff for Iranian Oil Field

Presenting a suitable hydrogel for water shutoff in Iranian oil field is based on gelation time and gel strength. Rheological behavior of gel was the main purpose of this research. To determine the gelation time, thermal stability and gel strength a plan of bottle tests were conducted by using the central composite design method with two factors. The hydrogels composed of AN125VLM and chromium triacetate as copolymer and crosslinker, respectively were recorded for 8 weeks. Furthermore, the consistency modulus of the gels was measured by amplitude sweep tests using the Paar-Physica universal spectrometer, model MCR501. A hydrogel with 26,339 ppm concentration of copolymer and 0.12 ratio of crosslinker/copolymer and also gelation time of 2 days was selected as the optimal one. Also, it showed the maximum value of consistency modulus of 31,900 Pa among the other samples, tremendously, which showed the highest resistance against external stress. To ensure the gel strength among different effective parameters on the gel in porous media, a plan of rheological experiments were carried out. A 12 Run Plackett-Burman design was used for screening the eight parameters of NaCl, CaCl2, KCl and MgCl2 concentrations, temperature, pH, sodium lactate and nanoclay while keeping the optimal hydrogel component constant. Finally, it was found that temperature was the most effective parameter to control gelation time and also pH had negligible effect on the gelation time of this optimal gel.

Reusability and regeneration of solid catalysts used in ultrasound assisted biodiesel production.

Reusability of two heterogeneous catalysts in ultrasound (US) assisted biodiesel production was investigated in comparison to each other. An ultrasound (US) generator (200 W, 20 kHz) equipped with a horn type probe (19 mm) was used. Regeneration experiments were planned according to second order central composite design (CCD) method. After the eighth use of the catalysts, biodiesel yield decreased from 99.1% to 90.4% for calcined calcite (CaO) and from 98.8% to 89.8% for calcined dolomite (CaO.MgO). Furthermore, regeneration of spent catalysts by calcination was investigated; optimum temperature and time were found as 750 °C and 90 min, lower than fresh catalyst preparation conditions. The regenerated catalysts were reused in a second process cycle; biodiesel yield was calculated as 97.2% for CaO and 96.5% for CaO.MgO. Finally, the process showed that calcination is an energetically favorable regeneration process of spent catalysts.

Prediction of tungsten insert gas welding process parameter using design of experiment and fuzzy logic

The focus of this study is to predict tungsten inert gas (TIG) welding process parameter such as heat input for stabilizing heat and removing post weld crack formation in mild steel weldment. The main input parameters examined are the welding current, voltage and speed whereas the measured (response) parameter is heat input. Statistical design of experiment was done by means of central composite design method using the range and levels of independent variables. The experiment was carried out 20 times (with 5 specimens per run) using 60 mm x 40 mm x 10 mm mild steel coupons. The plate samples were cut longitudinally with a Single-V joint preparation, with the edges beveled. The welding process utilizes 100% pure argon as a protecting gas to shield the weld specimen from external interaction. The interaction between the input and response variables was analyzed using a fuzzy logic system. The result showed that for a welding current, voltage and speed of 190 A, 21 V, and 2.0 mm/s respectively, the predicted heat input was 0.912 kJ/mm whereas for input parameters of (170 A, 25 V, and 2.0 mm/s) and (180 A, 23 V, 0.98 mm/s), the predicted heat inputs were 1.07 kJ/mm and 1.380 kJ/mm, respectively.

Split-plot Central Composite Experimental Design Method for Optimization of Cake Height to Achieve desired Texture

In many industrial experimental situations, the levels of certain factors under investigation are much harder to change than others due to time and/or cost constraints. An appropriate approach to such situations is to restrict the randomization of the hard-to-change (HTC) factors, which leads to a split-plot structure. This work designs and conducts a split-plot central composite experiment for optimizing cake height using oven temperature(Factor A) as the HTC factor, amount of flour (B), baking powder (C), and amount of milk (D) as the easy-to-change (ETC) factors. A second-order split-plot central composite design (CCD) model was fit to the generated data and analyzed using generalized least squares (GLS). A stationary point, which gives optimum cake height, was then determined. The results show that main effects of oven temperature, flour, baking powder, and milk were highly significant on the cake height . Their quadratic effects were also significant except that of the flour. The flower/baking powder interaction effect was significant. The fitted model accounted for about 95% of the total variability in the cake height data. The observed optimum cake height was ̂ at a stationary point: A . This study has established the potentials of response surface experiments in optimizing products in food industries.
 Keywords: Experiment, split-plot CCD, Cake height, Design, Stationary point.

Evaluating optimized electroless plating of carbon nano-fibers by nickel nanoparticles utilizing UV–Vis spectrophotometry and MCR–ALS chemometrics method

The purpose of this study was to optimize the nickel (Ni) coating of carbon nano-fibers (CNF) utilizing electroless plating (EP) method. A total number of 30 runs were studied in different experimental conditions such as temperature, time, reducing agent concentration and Ni concentration using central composite design (CCD) method. Pure CNF and functionalized CNF were characterized by Fourier transform infrared (FTIR) spectroscopy. The concentration of the Ni after plating was quantitatively analyzed by atomic absorption spectroscopy in order to determine the amount of coated Ni. Ni/CNF was also characterized by field emission scanning electron microscopy (FE-SEM) and X-ray fluorescent spectroscopy as well (XRF). The optimized experimental condition was determined as: 38.5 g/l concentration Ni, 65 min time, 60 °C temperature and 8.0 g/l reducing agent concentration. The nanostructure's size was estimated to be 29 nm as an optimized response of Ni/CNF. Eventually, the ultraviolet–visible (UV–Vis) spectroscopy in 200−800 nm spectral region along with multivariate curve resolution alternating least squares (MCR-ALS) chemometric technique was performed to evaluate the adsorption process.

Development of False Ceiling Board Using Indigenous Sheep Wool Fiber Reinforcement/Gypsum Matrix Composite

A false ceiling is a ceiling made beneath the main ceiling of the room, building and other related applications. In this research Composite ceiling board based on short wool fibers and gypsum was developed by hand lay-up process. A three-factor two-level experimental design central composite design method was applied to develop the composites and explore the contribution of each parameter on mechanical properties. The present investigation focuses on the physical and mechanical characterization of the sheep wool fiber-reinforced polymer-matrix composites and studies the development and Characterization of indigenous sheep wool fiber reinforced gypsum matrix composite. Gypsum (CaSO4.2H2O) as matrix and wool as reinforcement would be used as light-weight material for false ceiling board.

A bionic noise reduction strategy on the trailing edge of NACA0018 based on the central composite design method

New innovative approaches to reduce noise are of great significance in engineering. Taking the typical NACA0018 airfoil as the study target, the serrated treatment of the trailing edge is carried out with the bionic noise reduction technology. To boost the design efficiency and clarify the distributing laws between the design parameters and the noise performances, a special investigation about optimizing the NACA0018 airfoil’s serrated trailing edge is implemented here. Firstly, a united parametric approach to represent various design schemes is proposed. Then, with the integration of the Computational Fluid Dynamics/Computational Acoustics analysis, Central Composite Design, and the Respond Surface Method, a bionic noise reduction strategy is established. The findings can be gotten as: taking into account the existence of the casing or wall near the span-end of the airfoil, the newly defined install location adjustment factor has an influence on the reduction noise, and the install location adjustment factor mainly affects the near-wall flow field of the serrated trailing edge; the optimal design scheme is obtained successfully, which can reduce the overall sound pressure level by about 2 dB in relative to the target; through the mechanism analysis of noise reduction, it can be found that the serrated trailing edge can suppress the development of the laminar separation bubbles, so the noise is reduced.

Analysis and determination of the behavioral mechanism of rock bridges using experimental and numerical modeling of non-persistent rock joints

Presence of rock bridges (RB) in natural non-persistent discontinuity sets is an effective factor on the stability of rock structures. Investigations show that the bearing capacity of jointed rocks is changed with variation of different joint parameters. Therefore, in order to investigate the effect of parameters such as contact area and number of rock bridges, normal load, angle, length, and number of joints on shear strength of non-persistent rock joints and also to recognize the interaction of these parameters on the mechanical behavior of joints, experimental design methods of Taguchi and central composite design (CCD) were used for the sample generation, testing and numerical modeling. The effective parameters on the shear strength of jointed samples were obtained based on the previous studies. Using the Taguchi method, 16 samples were tested at CNL condition and the effect of parameters such as normal stress, number, and area of rock bridges was investigated. To evaluate other joints parameters, 30 experiments were designed using CCD method and examined using numerical modeling. Using the analysis of variance (ANOVA), it was found that the obtained models are statistically significant. According to the results of ANOVA, the area of rock bridges and the angle of joints showed the highest and the lowest effect on shear strength of coplanar and non-coplanar jointed samples, respectively. The results displayed that the dominant failure for planar non-persistent joints is pure shear and a combination of tensile and shear cracks is created from the both tips of adjacent joints. Moreover, it was found that the dominant failure of non-coplanar joints is the combination of shear and tension. Bonded particle model (BPM) and smooth joint model (SJM) were also applied for numerical modeling. A new method was considered for applying SJ to the models, which solved the interlocking problem during the test.

Predicting cyanobacterial decomposition response to multiple environmental factors through Central Composite Design method

Abstract The phenomenon of cyanobacterial blooms has always attracted attention worldwide, most of which focuses on the occurrence mechanism and treatment methods of cyanobacterial blooms. Research on the relationship between the decomposing process of cyanobacterial blooms and environmental factors is relatively rare. Notably, the interaction between multiple environmental factors has not been reported yet. Therefore, we studied the cyanobacterial decomposition response to various environmental factors through response surface method and central composite design method. In this study, the concentrations of microcystins were determined by high performance liquid chromatography; the algal cell density of M. aeruginosa was assessed using a Sedgewick–Rafter counting chamber under a microscopic magnification of × 200–400; chlorophyll a was analyzed spectrophotometry at wavelengths of 665 and 750 nm using phaeopigment correction; and the correlation coefficients of polynomial model were analysis using Design Expert 8.0.6 (Stat-Ease, Inc., Minneapolis, MN, USA) The environmental factors included temperature, pH, dissolved oxygen, light intensity, and cyanobacteria cell density. Verification tests showed that the final optimized phytochemical conditions of cyanobacteria decomposition were: temperature 35 °C, pH 6.5, dissolved oxygen 5.5 mg/L, light intensity 3000 lx, and cyanobacterial cell density 8.5 × 109 cells/L. The intensity of the influence of various environmental factors on R Chla and C MC−LR followed: pH > DO > Lux > T > cell density, and cell density > Lux > DO > T > pH, respectively. This study contributes to the theoretical knowledge of the prediction and analysis of the cyanobacterial decomposition status in different external environments.