Novel interface-enhanced bimetallic nanozyme-mediated magnetic integrated nanoplatform for integrated prevention and control of pathogenic bacteria.

Stereoselective synthesis of (S)-3-Hydroxy-3-phenylpropionate with a novel carbonyl reductase identified from a soda lake metagenome.

pH-adaptive evolution of glutamate decarboxylase enables gamma-aminobutyric acid biosynthesis without pH control.

Smartphone-enabled molecularly imprinted paper-based sensor for dual-mode detection of diclofenac sodium in Milk, beef and water.

Supramolecular engineering of high-efficiency nanozymes via chain-length-directed crystallization of cellulose oligomers.

Engineering and immobilization of imine reductase enable chemoenzymatic synthesis of SLC6A19 inhibitor JNT-517.

Over the past decade, the synthesis of alkynyl chalcogen derivatives has witnessed significant advances. Modern methodologies have increasingly focused on sustainable protocols, catalytic efficiency, milder reaction conditions, and atom-economical processes....

Multifunctionality analysis of serine hydroxymethyltransferases from human and Escherichia coli.

Interface-engineered Co5.47N-W5N4 heterostructure catalyst with synergistic dual-site hydrogen bonding and electronic modulation for efficient 5-hydroxymethylfurfural electrooxidation.

Investigation of moringa and guar gum based biopolymer support for the copper catalysis and its applications in A3-coupling reaction.

Co-catalysis of modified metal-organic framework immobilized enzyme with nonionic surfactant for biodiesel preparation.

Catalytic dehydrogenation efficiency of carbon quantum supported Ni nanoparticles and synthesized via dimethylformamide (DMF)

Self-powered detection of T4 polynucleotide kinase activity based on DNA structure transformation-modulated direct electron transfer of bilirubin oxidase.

Spatial-electronic dual stabilization of Pt-based intermetallic nanoparticles on defect-rich graphite domains for efficient oxygen reduction catalysis

Data-driven screening of transition metal C6N3 electrocatalysts for efficient oxygen reduction reaction catalysis

Enhanced synthesis of alanyl-glutamine dipeptide via molecular modification and process optimization of α-amino acid ester acyltransferase EAET.

Nano-piezoelectric BaTiO3 activate water to overcome pH limitations: A novel oxidase-mimicking strategy for cystathionine-γ-lyase colorimetric biosensing.

Amorphous MnO2 nanoflower with high laccase-like activity for colorimetric detection of pheochromocytoma biomarker.

BACKGROUND: Cold-adapted β‐galactosidases are valuable for low-temperature applications in biotechnology, food and pharmaceutical industries. OBJECTIVE: The study aimed to isolate novel cold-active β‐galactosidase-producing strains from marine sediments and develop more efficient and stable cold-active β‐galactosidase. MATERIALS AND METHODS: A cold-adapted stain ( Bacillus sp. K3) was isolated from deep-sea sediments. The enzyme gene was cloned and expressed in Escherichia coli BL21 (DE3). The enzyme was purified and characterized for its biochemical properties and transglycosylation potential. RESULTS: The recombinant β‐galactosidase exhibited optimal activity at 20°C and maintained stability from −20°C to 50°C, with peak efficiency at pH 6.0. Kinetic analysis confirmed high catalytic efficiency at low temperatures. Its activity was enhanced by Triton X‐100, Mg 2+ , and Mn 2+ , while strongly inhibited by Hg 2+ , Cu 2+ , EDTA, and DTT. Notably, the enzyme achieved a 13.7% galacto-oligosaccharide (GOS) conversion at 20°C. CONCLUSION: A cold-adapted stain ( Bacillus sp. K3) was isolated from deep-sea sediments, and it produces a β‐galactosidase that functions at low temperatures. The study highlights the potential of the K3 β‐galactosidase for low-temperature applications in biotechnology, food, pharmaceutics and environmental industries.

Ru clusters encapsulated with Al-rich zeolite for efficiently catalytic elimination of 1,2-dichloroethane.