Sour Cassava Starch Research Articles

Effect of gum extracts on the bread-making and textural properties of dough and bread made from sour cassava starch (Manihot esculenta), Peanut (Arachis hypogaea) and cowpea flour (Vigna unguiculata)

Gluten intolerance, as well as the scarcity of wheat flour in some parts of the world, has prompted the development of gluten-free bread. Gluten-free bread, on the other hand, results in a low specific volume and to remedy this, the use of hydrocolloids and bases has proved to be very successful. The current study aims to determine the optimal proportions of Triumffeta pentendra gum extract and bicarbonate in the breadmaking of a composite flour based on sour cassava starch, peanut flour, and cowpea flour. A Box Benkhen design was used to achieved this, with the variables being the amount of gum extract, the amount of bicarbonate, and the amount of water. The specific volume and texture properties were evaluated as responses. The specific volume was calculated using standard methods, and the textural properties were determined using a texture analyzer. It appears that the incorporation of gums extract, bicarbonate, and water significantly increased the specific volume. The incorporation rate of gum extract significantly increases the hardness, consistency, and masticability which decreases with the incorporation rate of bicarbonate and water. Cohesion and elasticity, on the other hand, increased with the incorporation rate of bicarbonate and water but decreased with the incorporation of gum extract. The optimal gum extract, bicarbonate, and water proportions are 0.28 g, 1.99 g, and 112.5 ml, respectively. As a result, the specific volume is 1.51cm3/g, the hardness is 38.51(N), the cohesion is 0.88, the consistency is 32.86(N), the elasticity is 5.57(1/L), and the masticability is 162.35(mj). According to this findings, gum extracts and sodium bicarbonate can be used to improve the quality of gluten-free bread made with sour cassava starch, peanut and cowpea flour.

Evaluating the Impact of Inulin Addition before Solar Drying on the Characteristics of Sour Cassava Starch as a Food Ingredient

AbstractSour cassava starch is obtained by spontaneous fermentation of the starch cake immediately after being extracted, followed by sun‐drying, where oxidation reactions take place that are essential for the enhanced baking expansion of this starch. The aim of this work is to evaluate the influence of adding a different polysaccharide to the starch cake before sun‐drying in the characteristics of the obtained product. Inulin is chosen for being a soluble prebiotic fiber that may improve the nutritional quality of the starch. Inulin modifies the oxidation of the fermented starch during sun‐drying, and forms a film around the granules that hinder their swelling and water retention capacity. The crystallinity of the inulin is increased by hydration and sun‐drying. Adding inulin to the fermented starch before sun‐drying reduces its expansion capacity, but in a lower rate than when inulin is added after sun‐drying. The fermented starch sun‐dried with inulin produces a baked snack with similar texture to that of the sour starch alone, and softer than that from the native starch.

Effect of Fermentation Time and Varietal Difference on the Pasting Properties and Bread‐Making Ability of Cassava Starch (Manihot esculenta)

AbstractThis research is to investigate the breadmaking ability of three cassava starch varieties (96/1414, TME15, and YARA) grown in Cameroon. To achieve this, starch samples from each variety are collected before and during fermentation to determine chemical, rheological, and bread‐making properties. They are analyzed for pH, titratable acidity (TTA), lactic acid (LA), specific volume (SPV), and pasting properties using known method; These parameters are used to perform a principal component analysis (PCA). The morphological characteristics are studied by scanning electron microscope (SEM). Result shows that, there is a decrease in pH (6.21–3.8) with an increase in TTA (0.34–7.05) and LA (0.15–6.46) with fermentation time. In parallel, a decrease of pasting properties and an increase in SPV (1.15–2.82 cm3 g−1) are observed. The PCA surrounds 96/1414 day 30, YARA day 30, and TME15 day 25 α 30 as the best samples, and the SEM shows a superficial degradation of the granules after fermentation. Thus, this study suggests that the optimal sour cassava starch for bread‐making can be obtained from 96/1414 day 30, TME15 day 25 α 30, and YARA day 30. The variety 96/1414 appears to have the best bread‐making ability while TME15 appears to be most suitable for short fermentation time.

Optimization of the Processing Conditions for the Production of a Gluten-Free Bread from Sour Cassava Starch (Manihot esculenta) and Some Legumes (Arachis hypogaea, Vigna unguiculata, and Glycine max).

Sour cassava starch is used as an alternative to wheat flour in breadmaking. However, its nutritional and technological properties are limited. To remedy this, the use of legumes has proved to be very successful. Thus, the present study aimed to determine the optimal condition for the production of bread made from sour cassava starch, peanut, cowpea and soybean flour. The I-optimal design was employed to obtain an optimal proportion of the mixture with the variables sour cassava starch, cowpea, soy and peanut flour. The responses evaluated were overall acceptability, specific volume and protein content. It resulted that the incorporation of sour cassava starch positively influenced the volume but negatively influenced the protein content and overall acceptability. While the addition of legumes increased protein content and overall consumer acceptability, the specific volume was reduced. The optimal proportions of sour cassava starch, cowpea, soybean and peanut flour were 64.11%, 18.92%, 0% and 16.96%, respectively. Under this condition, it led to a desirability of 1, specific volume of 1.35, overall acceptability of 6.13, protein content of 9.72%, carbohydrate content of 67.89%, fat content of 9.39%, fiber content of 2.10% and ash content of 1.04%. The findings suggest that cowpea and peanut can be used for the improvement of the technological, nutritional and sensory properties of sour cassava starch bread and thus increase its consumption and application in the food processing industry.

Modified Cassava Starches’ Identification through Mid-Infrared Spectroscopy and Exploratory Analysis

Different starch properties may cause alterations in the foodstuff’s external appearance. However, modification processes in starches are usually secretive. The use of chemically modified starches is regulated by international standards, which makes it important to identify its presence and type. Mid-infrared spectroscopy (MIR)-modified starches’ identification can be hindered by the presence of excess glucose. This research investigates types of modification in commercial starches and in approaches that circumvent MIR’s limitations with exploratory analysis. It also considers that enzymatic hydrolysis (α-amylase and amyloglucosidase) can highlight the points of modification in the structure, which can be detected with infrared assays. To determine if sour cassava starch (FCS) is modified and check its type, six samples were selected: a native one, three of the most common cassava modified starches (etherified, esterified, and FCS), and two laboratory processed samples (Acid, Oxidized). The results showed that the sour cassava starch showed similarities with a commercial ester and an oxidized cassava starch, which may be due to the formation of a graft, corresponding to what the literature has already reported for corn starch treated with lactic acid and gamma radiation.

Traditional sour cassava starch obtained with alterations in the solar drying stage

After natural fermentation and solar drying, sour cassava starch acquires expansion property. Traditionally, solar drying occurs without protection, and consequently physical contamination can arise. Thus, the aim of this study was to produce sour cassava starch using a low-cost solar dryer to prevent contamination, whilst retaining the expansion property. The samples were characterized in relation to chemical composition, microscopy and physical contaminants, as well as expansion, thermal, pasting, structural and technological properties. In addition, biscuits were prepared and compared with commercial samples. The solar dryer provided faster drying and lower moisture levels than the control. The specific volumes of the sun-dried products were similar to the control, with minimized contamination. The granules of the starches showed slight erosion on their surface, which was probably due to amylolytic enzymes. X-ray diffraction showed a typical A-type pattern. DSC analysis resulted in higher gelatinization temperatures and enthalpy changes for the sour cassava starch. The pasting properties showed a reduction in the viscosity peak at neutral pH. Although the cassava starch biscuits made with sour cassava starch dried in the solar dryer presented lower expansion levels in relation to the other biscuits produced with commercial sour cassava starch, they had desirable sensory characteristics.

A Natural Antifungal from Cassava Starch Fermentation Wastewater

Sour cassava starch production process, during the fermentation step, an acidic wastewater is generated, in this study named wastewater of cassava starch fermentation (WCSF). It has been studied the effect of WCSFs (from three industrial source: WCSF 1, 2 and 3) and patterns of organic acids (lactic, acetic, propionic and butyric acids, which were the main organic acids present in the WCSFs) singly or together on the development and growing of Botrytis cinerea, Monilinia fructicola and Colletotrichum gloeosporiodes. WCSFs showed antimicrobial effect on the three fungi studied. WCSF2 inhibited the germination of conidia from the three fungi, while WCSFs 1 and 3 inhibited conidia germination of B. cinerea and C. gloeosporiodes. The acetic, propionic, butyric and the mix acids were also effective in the reduction of conidial germination. In the analysis of mycelia growth, it has been observed that the growing was statically smaller, in relation to negative control, for all fungi in the treatments with the WCSFs and with the pattern solution of butyric acid. Which suggest that the WCSFs compounds and butyric acid are effective inhibitor for fungi and justify future studies using this wastewater.

Study and application of photo-modified cassava starch with lactic acid and UV-C irradiation

Sour cassava starch (SCS) is naturally modified through the joint action of anaerobic fermentation and sun-drying, but there appears be an immense variety in the quality of commercial SCS. The present study aimed to produce cassava starch with high expansion property (PCMS). After the establishment of the production conditions, PCMS was compared to commercial starches in terms of specific volume, carbonyl and carboxyl content, thermal and paste properties, as well as, about its use for the production of cheese bread. In order to obtain PCMS, a 5 × 5 factorial design was set to determine lactic acid concentrations and exposure times to UV-C irradiation. The optimal conditions for photo-modification were 20 g/kg of lactic acid concentration and exposure time at UV-C for 75 min. The specific volume of PCMS was 2.85 times higher than native cassava starch but did not differ from the starch with high expansion property. Compared to native cassava starch, the PCMS showed lower peak and setback viscosity, higher amylose content and alteration of gelatinization enthalpy. The cheese bread prepared with PCMS did not differ from those produced with the other two SCS; consequently, PCMS preserved and ensured product quality that may be destined for food industry.

Brazilian cheese bread rolls from fermented and native waxy maize starch

ABSTRACT Brazilian cheese bread (CB) rolls are gluten-free bread made from sour cassava starch. They are soft in the center and have crispy crust, reminding of the extruded structure. Although native waxy maize starch (WMS) has higher technologic quality, it has not been used in CB rolls; therefore, this study aimed to investigate the use of WMS and the backslopping fermentation process plus sun drying in WMS to replace sour cassava starch (0, 25%, 75%, and 100%) and the effects on dough development, color, and textural properties. Data were analyzed using variance and Tukey test (p ≤ 0.05%), and the results were set against a commercial product from cassava starch. The better formulation was characterized by proximal composition, sensorial acceptance, and willingness to buy, and data were presented as means and standard deviation. The different statistic parameters included color to redness coordinate, baking expansion capacity, specific volume, hardness, fracturability, and chewiness. The best formulation had 100% of sundried WMS and was microbiologically safe. The proximal composition had lower values than that obtained from the Brazilian Food Database. The product was considered satisfactory as it had a score of more than 7.79 to texture, appearance, and flavor with higher willingness to buy (92.5%). The backslopping fermentation plus SWMS was considered a potential replacement for sour cassava starch.

Microbiological and physicochemical profiles of the sour cassava starch and bagasse obtained from cassava agroindustry

The aim of this work was to evaluate the microbiological and physicochemical qualities of sour cassava starch and cassava bagasse produced during cassava agroindustry in order to evaluate their potential for use in food. All samples were kindly provided by family farmers producers from the region of Cará, in town of Bela Vista de Goiás, Brazil. All of sour cassava starch samples showed microbial counts within the values established by the current legislation. Drying process of raw cassava bagasse to produce the bran was sufficient to reduce the mold and yeast counts (from 1.9x103 to 3.5x101 CFU g -1) and of total coliform counts (from 2.1x103 to 2.4x102 MPN 100 mL-1), and also eliminated the contamination by coliforms at 45 ° C and Bacillus cereus. Carbohydrate contents of all sour cassava starch samples ranged from 99.64 to 99.75 g 100g-1. These results indicated that the sour cassava starch presented high degree of purity, and extraction process was efficient. Bran sample showed high carbohydrate (96.94 g 100g-1) and dietary fiber (22.58 g 100g-1) contents. High dietary fiber content suggests the use of bran as raw material to increase the availability of fiber in food products, increasing its nutritional quality.

Low Density Wood Particleboards Bonded with Starch Foam-Study of Production Process Conditions.

It has been shown that wood particleboards bonded with sour cassava starch can display low density combined with good physico-mechanical performance, thanks to starch being able to produce a strong foam that fills the interparticular space. Here we optimize the pressing conditions for the production of these panels. The procedure involved hot-plate pressing in two stages: (1) lowering the top platen to a specified thickness for a duration designated as pressing time, followed by (2) raising the top platen to allow panel expansion for a duration designated as hold time. The parameters studied were the pressing time (10 to 150 s), the hold time (290 to 890 s), and the top platen temperature (80 to 190 °C). The hold time and pressing time showed to be crucial parameters. The best operating conditions corresponded to 600 s of press cycle time, comprising 60 s of pressing time and 540 s of hold time. The top platen temperature used was 190 °C. The particleboards produced had a density of 405 kg·m−3, an internal bond strength of 0.44 N·mm−2, and a thickness swelling of 13.2%. This can be considered as very good performance, taking into account the panels’ low density.

Antagonistic lactic acid bacteria in association with Saccharomyces cerevisiae as starter cultures for standardization of sour cassava starch production

In order to improve cassava's palatability and reduce its toxicity, this root is fermented and applied in foods, such as sour cassava starch used to prepare cheese bread and biscuits. This fermentation occurs spontaneously with lactic acid bacteria (LAB) and yeasts. However, it remains an empirical process, with long duration and lack of product quality homogeneity. This work aims to use starter cultures in a pilot-scale fermentation process for the production of sour cassava starch. After differentiation of strains, Lactobacillus plantarum Lp3, which exhibited great total titratable acidity (TTA) (5.01 ± 0.05%) and antagonistic activity against Bacillus cereus, Escherichia coli and Salmonella Typhimurium, together with Lactobacillus brevis Lb9 (with lesser TTA values: 2.71 ± 0.10%, but amylolytic activity: 2.75 ± 0.61mm) were tested as single and co-cultures with Saccharomyces cerevisiae UFMG-A1007. LAB and yeasts were inoculated at counts of 8 and 7 log10 CFU/g, respectively, and they remained until the 28th day only in co-culture, highlighting the importance of the yeast for the LAB viability. Although single cultures lead to higher acidity during fermentation, the final product acidity obtained with single cultures did not differ from the acidity obtained with L. plantarum Lp3 in association with S. cerevisiae UFMG-A1007. Therefore, this co-culture exhibited higher potential to be tested as a starter culture in industrial-scale fermentation studies because both microorganisms were in high counts until the end of fermentation and contributed to a final product safe for human consumption, with satisfactory acidity, expansion capacity, and physicochemical properties.

Ozone technology as an alternative to fermentative processes to improve the oven-expansion properties of cassava starch

Cassava starch has a remarkable importance in the food industry and it can be used either as ingredient or additive. The sour cassava starch is a product with a good oven-expansion capacity and several applications, being especially important as an alternative for gluten-based products. However, it is obtained through natural fermentation followed by solar drying, which hinders its production and application. In this context, this work proposed the ozone processing as an alternative to obtain cassava starch with good oven-expansion property. The effect of ozonation on starch granular and molecular structure was evaluated, as well as on the main starch properties. The ozone processing reduced the starch molecular size, also forming carbonyl and carboxyl groups. The structural changes led to pastes with higher clarity, better oven expansion and softer texture. The improvement in starch properties and technological aspects were related with the molecular changes. In conclusion, ozone oxidation was proved to be a viable and easier alternative for the conventional process.

Modification of the Fermentation Process for Sour Cassava Starch with Expansion Properties

The present research aimed to evaluate the effect of the manipulation of the process variables during the natural fermentation of the cassava native starch. In Colombia, the production process is carried out with a traditional and low-tech method in small companies called

Fermentation and drying effects on bread-making potential of sour cassava and ahipa starches

Cassava sour starch is a gluten-free product obtained by natural fermentation and sun-drying that outstrips the native starch baking expansion properties. Although maize starch has been subjected to a similar process, this desirable feature could not be achieved. Ahipa, an unexploited tuberous root, renders starch with relatively low gelatinization temperature and amylose content, like that of cassava. The aim of this work was to study the characteristics and technological properties of ahipa starch subjected to different fermentation processes and drying methods (oven- or sun-drying) and compare the bakery quality of its derived products to those from fermented cassava starches.Ahipa starch followed similar fermentation paths to those followed by cassava's, and sun-drying significantly reduced the content of the resultant lactic and butyric acids. Rheological behavior of starch pastes as well as moisture content and hardness of the doughs obtained from fermented and sun-dried ahipa starches differed from those of cassava. Sun-light exposure resulted detrimental for the expansion properties of ahipa sour starches, while the native one showed baking expansion properties like those of fermented sun-dried cassava starch.Thus, ahipa starch represents an interesting ingredient for the elaboration of gluten-free baked products.

Gluten-free baked foods with extended shelf-life.

The objective of this study was to develop a formulation that can retard staling/retrogradation of cheese breads without compromising their texture and expansion properties. During baking the dough expands and becomes soft, with lower density. Binary mixtures of cassava starch (Native + Oxidized) and with guar gum (Oxidized + Guar Gum) as well as a ternary mixture (Native + Oxidized + Guar Gum) were prepared. The mixtures were analyzed for freeze-thaw stability, expansion, pasting, thermal structural and retrogradation properties. The results were compared with those of sour cassava starch (polvilho azedo-PA), native cassava starch (N) and oxidized cassava starch (O). Moreover, cheese breads were prepared with these mixtures and evaluated during storage. The ternary mixture N + O + GG showed superior freeze-thaw stability (syneresis of 4.9, 7.8 and 11.0% in 1st, 2nd and 3rd cycles, respectively); the low retrogradation of this sample was confirmed both by DSC and FTIR analyses. The sample N + O + GG had a high expansion (> 10mL/g) and the cheese breads developed with this mixture had a slower staling. Our results confirmed that the mixture N + O + GG can improve formulations of gluten-free baked foods.

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espanolComercialmente las enzimas de sacarificacion son mas costosas que las de licuefaccion, por tal razon se buscan estrategias que permitan el suministro de estas enzimas a bajo costo. Los objetivos de este estudio fueron evaluar extractos enzimaticos sacaroliticos a partir de cepas nativas, seleccionar un extracto y determinar las mejores variables para la produccion de jarabes de glucosa. Para ello, en trece hongos filamentosos aislados de yuca amarga Manihot sp. fueron evaluadas la actividad sacarolitica, la hidrolisis de maltosa, la produccion de glucosa y la formacion de biomasa bajo condiciones de fermentacion sumergida. El aislamiento identificado como Aspergillus A1 fue seleccionado por presentar la mas alta actividad volumetrica (0,09UI.L-1). Durante la fermentacion en estado solido se corroboro la presencia de proteinas mediante el metodo de electroforesis SDS-PAGE y se evidencio una banda de mayor intensidad con peso molecular entre 60 y 70 kDa. Para el extracto enzimatico de Aspergillus A1 se determino que las mejores condiciones experimentales de sacarificacion, con el uso de maltosa como sustrato, fueron pH 4,0, temperatura 55,0 °C, y sin agitacion. Igualmente en la evaluacion del efecto de los cofactores Cu2+, K+, Mg2+ y Na+ en concentraciones de 1 mM, se observo que todos incrementan la actividad enzimatica especialmente el Mg2+ , el cual la aumenta en 1,32 UI.mg-1 (32,4%). La estabilidad termica de la proteina a 55,0°C fue de 120 minutos. La capacidad sacarolitica del extracto enzimatico fue confirmada usando como sustrato almidon de yuca amarga licuado. EnglishThe saccharification enzymes are more expensive than liquefaction enzymes for that reason are sought strategies that allow the supply of these enzymes at low cost. The objectives of this study were to evolve saccharolytic enzymatic extracts from native strains, to select an extract and to determine the best variables for the production of glucose syrups from liquefied bitter cassava starch.Thirteen mushrooms isolated from sour cassava (Manihot sp). were evaluated for their saccharolytic activity, hydrolysis of maltose, glucose production and biomass formation in submerged fermentation. Aspergillus A1 was selected because it had the highest volumetric activity (0.09UI.L-1). During solid-state fermentation, the presence of proteins was corroborated by electrophoresis SDS-PAGE. Through various experiments, the best experimental condition were pH 4.0, agitation 0r.p.m and temperature 55.0°C; the effect of cofactors Cu2+, K+, Mg2+ and Na+ was evaluated, Mg2+ increases the activity in 1.32UImg-1 (32.4%). The thermal stability at 55.0°C is 120 minutes. Finally, the saccharolytic capacity of the enzymatic extract was confirmed using liquefied cassava starch. portuguesComercialmente as enzimas de sacarificacao sao mais custosas do que as de liquefacao, por esta razao procuram-se estrategias que permitam fornecer este tipo de enzimas a baixo custo. Os objetivos deste estudio foram avaliar extratos enzimaticos sacarolīticos de cepas nativas, selecionar um extrato e determinar as melhores variaveis para a producao de xaropes de glicose. Para isto, de treze fungos filamentosos isolados de mandioca amarga Manihot sp., foram avaliadas a atividade sacarolitica, a hidrolise de maltose, a producao de glicose e a formacao de biomassa em condicoes de fermentacao submersa. O isolamento Aspergillus A1 foi selecionado por apresentar a maior atividade volumetrica (0,091UI.L-1). Durante a fermentacao em superficie a presenca de proteinas foi confirmada pelo metodo de eletroforese de SDS-PAGE junto com uma banda de maior intensidade com peso molecular entre os 60 e 70kDa. Para os preparados enzimaticos de Aspergillus A1 determinou-se que as melhores condicoes experimentais de sacarificacao usando maltose como substrato foram pH 4,0, temperatura de 55,0°C, sem agitacao. Igualmente na avaliacao do efeito dos cofatores Cu2+, K+, Mg2+ e Na+ em concentracoes de 1 mM, observou-se que todos incrementam a atividade enzimatica principalmente o Mg2+ em 1,32UI.mg-1 (32,4%) em relacao ao controle. A estabilidade termica da proteina de 55,0°C e de 120 minutos. A capacidade sacarolitica do extrato enzimatico foi confirmada usando substrato hidrolisado de mandioca amarga.

Technological properties of sour cassava starches: Effect of fermentation and drying processes

Cassava sour starch is a naturally-fermented, sun-dried product. It allows obtaining less dense bakery products than the native starch, being an appreciated gluten-free ingredient. The aim of this work was to study the effect of fermentation and drying method on the physicochemical and technological characteristics of cassava sour starch and to identify the relationships among those distinctive features that may condition their potential food applications.Natural fermentation following starch extraction could not be replaced by the use of a starter. Sour starches pastes showed lower apparent viscosities related to their lactic and/or butyric acid content, and a higher retrogradation tendency, mainly associated to sun-drying. The fermentation combined with the solar exposition represent a complex process that induce changes not easily observed on starch powders but clearly evidenced after gelatinization of the starch suspensions.

Selection of starter cultures for the production of sour cassava starch in a pilot-scale fermentation process.

Sour cassava starch (Polvilho azedo) is obtained from a spontaneous fermentation conducted by microorganisms from raw materials and fermentation tanks. This product is traditionally used in the baking industry for the manufacture of biscuits and Brazilian cheese breads. However, the end of fermentation is evaluated empirically, and the process occurs without standardization, which results in products of inconsistent quality. Predominant microbiota from a cassava flour manufacturer was isolated in order to select starter cultures for the production of sour cassava starch in a pilot-scale fermentation process. Lactic acid bacteria and yeasts were isolated, enumerated and grouped by Restriction Fragment Length Polymorphism, and PCR fingerprinting, respectively. One isolate of each molecular profile was identified by sequencing of the rRNA gene. LAB were prevalent throughout the entire process. Lactobacillus brevis (21.5%), which produced the highest values of acidity, and Lactobacillus plantarum (13.9%) were among the most frequent species. Pichia scutulata (52.2%) was the prevalent yeast and showed amylolytic activity. The aforementioned species were tested as single and mixed starter cultures in a pilot-scale fermentation process for 28 days. L. plantarum exhibited better performance as a starter culture, which suggests its potential for the production of sour cassava starch.

Low Density Wood-Based Particleboards Bonded with Foamable Sour Cassava Starch: Preliminary Studies.

This work investigates the feasibility of producing low density particleboards using an adhesive system based on sour cassava starch, taking advantage of its adhesive and self-expansion properties. Relevant properties of the produced particleboards were evaluated according to European Standards including: density, internal bond, moisture content and thickness swelling. Low density particleboards were produced with densities between 207 kg/m3 and 407 kg/m3. The best performance corresponded to particleboard with a density of 318 kg/m3, an internal bond strength of 0.67 N/mm2, and a thickness swelling of 8.7%. These values meet the standard requirements of general purpose lightweight boards for use in dry conditions. Heat post-treatment (24 h at 80 °C) led to lower internal bond strength, due to retrogradation (recrystallization of amylose and amylopectin chains upon cooling) causing higher rigidity of the starch binder. However, it showed to have a significant effect on decreasing the thickness swelling.