Carya Cathayensis Research Articles

LC-MS/MS targeting analysis of terpenoid metabolism in Carya cathayensis at different developmental stages

LC-MS/MS targeting analysis of terpenoid metabolism in Carya cathayensis at different developmental stages

Sonication-synergistic natural deep eutectic solvent as a green and efficient approach for extraction of phenolic compounds from peels of Carya cathayensis Sarg

Sonication-synergistic natural deep eutectic solvent as a green and efficient approach for extraction of phenolic compounds from peels of Carya cathayensis Sarg

Response Surface Optimization of Enzymatic Hydrolysis of Peptides of Chinese Pecan (Carya cathayensis) and Analysis of Their Antioxidant Capacities and Structures

Pecan cake, a by-product of pecan processing, has not been fully developed and utilized. It contains proteins with high nutritional value that can be used as a potential source of bioactive peptides. In this study, the compound protease from the four common proteases was selected as the best protease, and the best hydrolysis was determined via response surface optimization. The optimal hydrolysis conditions were as follows: 55 ℃, pH 7.7, 3900 U/g protease concentration, and 2 h. Under these conditions, peptide yield and total antioxidant capacity (TAC) were 34.1 % ± 0.2 % and 1.1 ± 0.1 mmol/g, respectively. The peptides showed desirable 1,1-diphenyl-2-picrylhydrazyl (DPPH) (IC50: 0.2 mg/mL) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) (IC50: 0.4 mg/mL) radical scavenging capacities, which were found to be related to their amino acid compositions, molecular weights, and structures. The molecular weight of the peptides obtained from the pecan cake protein hydrolyzed by the compound protease was < 3 KDa, with their contents reaching 94.0 % ± 0.4 %. The yield of protein hydrolysate from pecan cake was about 7.2 %. HPLC analysis revealed that the content of hydrophobic amino acids in the peptides was relatively high. Fourier transform infrared spectroscopy and X-ray diffraction analysis showed that hydrolysis gradually changed the structure of the proteins from ordered to disordered, thereby exposing more antioxidant groups. α-helix and β-turn angles decreased from 29.5 % and 24.9–20.3 % and 15.2 %, respectively. β-sheet, β-antiparallel, and random coil increased from 24.2 %, 7.7 %, and 13.7–33.5 %, 9.3 %, and 21.8 %, respectively.

Carya cathayensis leaf extract attenuates ectopic fat deposition in liver, abdomen and aortic arch in ovariectomized rats fed a high-fat diet

Carya cathayensis leaf extract attenuates ectopic fat deposition in liver, abdomen and aortic arch in ovariectomized rats fed a high-fat diet

Phytophthora cathayensis sp. nov., a new species pathogenic to Chinese Hickory (Carya cathayensis) in southeast China.

Crown decline and mortality associated with collar lesions were observed on Carya cathayensis (Chinese hickory) trees in a plantation in Zhejiang province, China. Examination of active lesions resulted in the isolation of a homothallic, papillate Phytophthora sp. Detailed morphological and physiological studies and phylogenetic analysis, using ITS, beta-tubulin, cytochrome oxidase I, and heat shock protein 90 gene regions, revealed that all isolates belonged to an undescribed species residing in phylogenetic Clade 4, which is described here as Phytophthora cathayensis sp. nov. Inoculation trials were conducted under greenhouse conditions on C. cathayensis and C. illinoensis (pecan) plants to fulfill Koch postulates and hypothesize a possible pathway of the incursion. An existing report of a Phytophthora species with the same ITS sequence was reported on C. illinoensis from the USA in 2009. The difference in susceptibility of the two inoculated Carya species, and the report from the USA, suggest a possible introduction with plant material from the USA to China. Citation:Morales-RodríguezC,WangY,MartignoniD,VanniniA(2020).Phytophthora cathayensis sp. nov.,anewspeciespathogenictoChineseHickory(Carya cathayensis) in southeast China. Fungal Systematics and Evolution 7:99-111.doi:10.3114/fuse.2021.07.05.

Polyphenols Extracted from Chinese Hickory (Carya cathayensis) Promote Apoptosis and Inhibit Proliferation through the p53-Dependent Intrinsic and HIF-1α-VEGF Pathways in Ovarian Cancer Cells.

Ovarian cancer is the second most common gynecologic cancer with an estimated 13,940 mortalities across the United States in 2020. Natural polyphenols have been shown to double the survival time of some cancer patients due to their anticancer properties. Therefore, the effect of polyphenols extracted from Chinese hickory seed skin Carya cathayensis (CHSP) on ovarian cancer was investigated in the present study. Cell viability results showed that CHSP is more effective in inhibiting ovarian cancer cells than normal ovarian cells, with the IC50 value for inhibition of cell proliferation of Ovarian cancer cells (OVCAR-3) being 10.33 ± 0.166 μg/mL for a 24 h treatment. Flow cytometry results showed that the apoptosis rate was significantly increased to 44.21% after 24 h treatment with 20 μg/mL of CHSP. Western blot analysis showed that CHSP induced apoptosis of ovarian cancer cells through a p53-dependent intrinsic pathway. Compared with control values, levels of VEGF excreted by OVCAR-3 cancer cells were reduced to 7.87% with a 40 μg/mL CHSP treatment. Consistent with our previous reports, CHSP inhibits vascular endothelial growth factor (VEGF) secretion by regulating the HIF-1α-VEGF pathway. In addition, we also found that the inhibitory effect of CHSP on ovarian cancer is related to the up-regulation of Phosphatase and tension homolog (PTEN) and down-regulation of nuclear factor kappa-B (NF-kappa B). These findings provide some evidence of the anti-ovarian cancer properties of CHSP and support the polyphenols as potential candidates for ovarian cancer adjuvant therapy.

An appropriate ratio of unsaturated fatty acids is the constituent of hickory nut extract for neurite outgrowth in human SH-SY5Y cells.

Hickory nuts (Carya cathayensis Sarg, CCS), a well‐known Chinese medicinal nut, is thought to improve memory in Chinese folks. However, functional constituents have not been scientifically identified. In this study, human SH‐SY5Y cells, combined with Q‐TOF mass spectrometry (Q‐TOF‐MS) and standard substances, were used to evaluate the function in neuronal development and to identify constituents of CCS hydrophobic extracts (CCS‐HE). Data showed that CCS‐HE but not the control induced neurite outgrowth of SH‐SY5Y cells in a dose‐dependent manner, supported by which CCS‐HE induced the expression of nerve growth factor (NGF), neurofilament 160 (NF160), and neuronal peptide Y (NPY) mRNA. Q‐TOF‐MS analysis with standard substances indicated that linolenic acid (LNA), linoleic acid (LA), and oleic acid (OA) were the main constituents in CCS‐HE. Furthermore, mixtures of these unsaturated fatty acids (UFAs) at the natural ratio (1:8:16) significantly induced neurite outgrowth and gene expression of NGF, NF160, and NPY in a dose‐dependent manner. However, the individual and alternative ratios were not effective to induce the neurite outgrowth and gene expression of NGF, NF160, and NPY. These data implicate that an appropriate ratio of UFAs is the main constituent for the neurite outgrowth.

Antifungal activity of rapamycin on Botryosphaeria dothidea and its effect against Chinese hickory canker.

The trunk canker caused by Botryosphaeria dothidea is a devastating disease for Chinese hickory (Carya cathayensis) in China. Chemical fungicides are commonly and repeatedly applied to control the disease; however, fungicide application raises major environmental and food safety issues. Ecofriendly biocontrol alternatives were urgently needed. Herein, the antifungal activity of a natural secondary metabolite, rapamycin, against B. dothidea and the effect of a rapamycin-producing Streptomyces hygroscopicus on C. cathayensis canker were investigated. The 50% effective concentrations (EC50 ) of rapamycin against mycelial growth and spore germination on the potato dextrose agar were 5.1 × 10-3 and 5.5× 10-2 μg mL-1 , respectively, which were much lower than the EC50 values of thiophanate-methyl. The hyphae of B. dothidea exhibited premature aging and wrinkling after treatment with rapamycin at 5.0 × 10-3 μg mL-1 . A rapamycin-producing bacterium S. hygroscopicus LYJ637 was batch produced and formulated in a carboxymethylcellulose/poly (vinyl alcohol) (CMC/PVA) blend and used for testing the efficiency of the bacterium in controlling Botryosphaeria canker in C. cathayensis. S. hygroscopicus exhibited high stability in the CMC/PVA blend. Results of a 3-year field experiment suggested that rapamycin formation reduced the occurrence of both developed cankers and new cankers, with an efficacy comparable to the treatment with thiophanate-methyl. The rapamycin-producing S. hygroscopicus LYJ637 carried in a CMC/PVA blend prevented effectively Botryosphaeria canker on Chinese hickory, which provides an alternative approach to chemical control strategies.

Cca-miR398 increases copper sulfate stress sensitivity via the regulation of CSD mRNA transcription levels in transgenic Arabidopsis thaliana.

MicroRNAs play crucial roles during the process of plant development under stress conditions. Copper is an essential micronutrient for most organisms and serves as an important redox-active cofactor for various functional proteins. In the present study, we investigated the effects of copper sulfate stress on hickory (Carya cathayensis) root development. We identified that hickory cca-miR398 was related to copper sulfate stress response, targeting Copper/Zinc superoxide dismutases (cytosolic (CSD1) and chloroplastic (CSD2)) and a 5b subunit of mitochondrial cytochrome C oxidase (COX5b.1) that are linked directly to stress regulatory networks. The sequence of hickory cca-miR398 is highly similar to that of Arabidopsis miR398b and miR398c, regardless of one nucleotide variation. Therefore, target genes of cca-miR398 were investigated by using 5′-Rapid-amplification of cDNA ends. An overexpression of cca-miR398 in Arabidopsis caused a reduction not only in root length and cotyledon greening, but also in the CSD1, CSD2, and CSD3 transcription levels. These reductions had greater significance in transgenic Arabidopsis than in wild-type Arabidopsis under copper sulfate stress. The level of physiological indicators also changed in transgenic Arabidopsis. In addition, the expressions of copper-responsive microRNAs, such as miR397 and miR408, were affected by the copper sulfate stress. These results showed that CSD possesses the ability to enhance copper sulfate stress response in both transgenic Arabidopsis and hickory roots by increasing the production of superoxide dismutase. Our results also demonstrated that cca-miR398 weakens hickory tolerance to copper sulfate by regulating CSD targets.

Identification and expression analysis of auxin-responsive GH3 family genes in Chinese hickory (Carya cathayensis) during grafting.

The GH3 genes play vital roles in auxin homeostasis by conjugating excess auxin to amino acids. However, how GH3 genes function during grafting in Chinese hickory (Carya cathayensis) is largely unknown. Here, based on the transcriptome database, a comprehensive identification and expression profiling analysis of 12 GH3 genes in Chinese hickory were performed. Phylogenetic analysis indicated that CcGH3-x exists in a specific subfamily. To understand the roles of CcGH3 genes, tissue-specific expression and the response to different phytohormones were determined. Expression profiles of GH3 genes of Chinese hickory during grafting were analysed. The data suggested that 10 CcGH3 genes were down-regulated at an early stage of grafting, indicating that auxin homeostasis regulated by the CcGH3 family might be inhibited at initial stages. At the completion of grafting, expression levels of members of the CcGH3 family were restored to normal levels. Endogenous auxin levels were also measured, and the data showed that free auxin decreased to the lowest level at an early stage of grafting, and then increased during grafting. Auxin amino acid conjugation increased at an early stage of grafting in rootstock, and then decreased with progression of the graft union. Our results demonstrate that the reduced expression of CcGH3 family genes during grafting might contribute to the release of free auxin, making an important contribution to the recovery of auxin levels after grafting.

Professor YANG Jun's experience of infantile spastic cerebral palsy treated with the combination of acupuncture and herbal medicine

Professor YANG Jun believes that infantile cerebral palsy is located in the brain and closely related to the kidney. The clinical treatment should focus on the brain theory and root at the kidney. In pathogenesis, infantile spastic cerebral palsy refers to flaccidity of yang and spasticity of yin. The principle of treatment should be balancing yin and yang, promoting the circulation of the governor vessel and regulating the spirit/mind. In clinical treatment, the comprehensive therapy of acupuncture and herbal medicine is adopted. In acupuncture, the acupoints on the head and the face are dominant and the body acupoints are selected rigorously and precisely. The herbal formula with Carya cathayensis Sarg and Scorpio is used and taken orally with warm water. In acupoint application treatment, Borneolum Syntheticum and Radix et Rhizoma Asari are the main herbal medicines for the external application at Shenque (CV 8) and Baihui (GV 20). All of the above therapies are used in combination to co-achieve the effect of regaining consciousness, opening orifices and benefiting the intelligence. The clinical therapeutic effect of this comprehensive therapy is significant.

Genome-wide identification of lncRNAs during hickory (Carya cathayensis) flowering.

Non-coding RNAs with lengths greater than 200bp are known as long non-coding RNAs (lncRNAs), and these RNAs play important role in gene regulation and plant development. However, to date, little is known regarding the role played by lncRNAs during flowering in hickory (Carya cathayensis). Here, we performed whole transcriptome RNA-sequencing of samples from hickory female and male floral buds, in which three samples (H0311PF, H0318PF, and H0402PF) represent pre-flowering, flowering, and post-flowering, respectively, while eight male samples collected from May 8th to June 13th as this time course are the key stage for male floral bud differentiation. We identified 2163 lncRNAs in hickory during flowering, containing 213 intronic, 1488 intergenic, and 462 antisense lncRNAs. We noticed that 510 and 648 lncRNAs were differentially expressed corresponding to female and male floral buds, respectively. And some of the lncRNAs were in a tightly tissue-specific or stage-specific manner. To further understand the roles of the lncRNAs, we predicted the function of the lncRNAs in cis- and trans-acting modes. The results showed that 924 lncRNAs were cis-correlated with 1536 protein-coding genes, while 1207 lncRNAs co-expressed (trans-acting) with 7432 protein-coding genes (R > 0.95 or R < - 0.95). These lncRNAs were all enriched in flower development-associated biological processes, i.e., circadian rhythm, vernalization response, response to gibberellin, inflorescence development, floral organ development, etc. To further understand the relationships between lncRNAs and floral-core genes, we build a co-expressing lncRNA-mRNA flowering network. We classified these floral genes into different pathway (photoperiod, vernalization, gibberellin, autonomous, and sucrose pathway) according to their particular functions. We found a set of lncRNAs that preferentially expressed in these pathways. The network showed that some lncRNAs (i.e., XLOC_038669, XLOC_017938) functioned in a particular flowering time pathway, while others (i.e., XLOC_011251, XLOC_04110) were involved in multiple pathway. Furthermore, some lncRNAs (i.e., XLOC_038669, XLOC_009597, and XLOC_049539) played roles in single or multiple pathways via interaction with each other. This study provides a genome-wide survey of hickory flower-related lncRNAs and will contribute to further understanding of the molecular mechanism underpinning flowering in hickory.

Photosynthetic Mechanisms of Metaxenia Responsible for Enlargement of Carya cathayensis Fruits at Late Growth Stages.

Fruits of hickory (Carya cathayensis) are larger and their peel is greener after interspecific pollination by pecan (Carya illinoinensis; later pp fruits) than after intraspecific pollination by hickory (later ph fruits). Previous studies have found little genetic differences between offspring and their maternal parent, indicating that the observed trait differences between pp and ph fruits are due to metaxenia. Fruit development depends on the amount of photosynthetic assimilate available. Since there is no difference in photosynthesis of the associated leaves between pp and ph fruits, the larger size of the pp fruits might be attributed to changes in fruit photosynthesis caused by the different pollen sources. To elucidate to the photosynthetic mechanisms behind the metaxenia effect on fruit development in hickory, the effects of intraspecific and interspecific pollination regimes were examined in the present study. We observed the photosynthetic capacity in the peel of fruits and the related ecophysiological and morphological traits of both ph and pp fruits over a period of 120 days after pollination. Significant differences in the appearance and dry weight between ph and pp fruits were observed at 50 days after pollination (DAP). More than 70% of dry matter accumulation of the fruits was completed during 60–120 DAP, while the true photosynthetic rate of the associated leaves significantly decreased by about 50% during the same period. In several cell layers of the peel, the number of chloroplasts per cell was significantly higher in pp than in ph fruits. Similarly, the ribulose 1, 5-bisphosphate carboxylase (Rubisco) activity, the total chlorophyll content, and the nitrogen content were all significantly higher in pp than in ph fruits during all growth stages; and all of these physiological quantities were positively correlated with the gross photosynthetic rate of the fruits. We conclude that the enhanced photosynthetic capacity of pp fruits contributes to their fast dry matter accumulation and oil formation. This result will provide a theoretical basis for improving hickory fruit yields in practical cultivation.

Phytosterols extraction from hickory (Carya cathayensis Sarg.) husk with a green direct citric acid hydrolysis extraction method

Phytosterols extraction from hickory (Carya cathayensis Sarg.) husk with a green direct citric acid hydrolysis extraction method

Melatonin regulates the functional components of photosynthesis, antioxidant system, gene expression, and metabolic pathways to induce drought resistance in grafted Carya cathayensis plants

Melatonin regulates the functional components of photosynthesis, antioxidant system, gene expression, and metabolic pathways to induce drought resistance in grafted Carya cathayensis plants

Risk assessment, spatial patterns and source apportionment of soil heavy metals in a typical Chinese hickory plantation region of southeastern China

Risk assessment, spatial patterns and source apportionment of soil heavy metals in a typical Chinese hickory plantation region of southeastern China

Quantitative succinyl-proteome profiling of Chinese hickory (Carya cathayensis) during the grafting process

BackgroundChinese hickory (Carya cathayensis) is a popular nut plant having high economic value. Grafting is applied to accelerate the transition from vegetative phase to reproductive phase. Lysine succinylation occurs frequently in the proteins associated with metabolic pathways, which may participate in the regulation of the grafting process. However, the exact regulatory mechanism underlying grafting process in Chinese hickory has not been studied at post-translational modification level.ResultsA comprehensive proteome-wide lysine succinylation profiling of Chinese hickory was explored by a newly developed method combining affinity enrichment and high-resolution LC-MS/MS. In total, 259 succinylation sites in 202 proteins were identified, representing the first comprehensive lysine succinylome in Chinese hickory. The succinylation was biased to occur in the cytosolic proteins of Chinese hickory. Moreover, four conserved succinylation motifs were identified in the succinylated peptides. Comparison of two grafting stages of Chinese hickory revealed that the differential expressed succinylated proteins were mainly involved in sugar metabolism, carbon fixation, amino acid metabolism and plant-pathogen interaction. Besides, seven heat shock proteins (HSPs) with 11 succinylation sites were also identified, all of which were observed to be up-regulated during the grafting process.ConclusionsSuccinylation of the proteins involved in amino acid biosynthesis might be required for a successful grafting. Succinylated HSPs might play a role in stress tolerance of the grafted Chinese hickory plants. Our results can be a good resource for functional validation of the succinylated proteins and a starting point for the investigation of molecular mechanisms during lysine succinylation occurring at grafting site.

Melatonin Stimulates Activities and Expression Level of Antioxidant Enzymes and Preserves Functionality of Photosynthetic Apparatus in Hickory Plants (Carya cathayensis Sarg.) under PEG-Promoted Drought

Nowadays, drought is one of the major abiotic factors which negatively affects growth and development of several fruit tree species, including Chinese hickory plants (Carya cathayensis Sarg.). The present investigation was conducted to study the possible positive effects of melatonin in drought resistance of C. cathayensis plants along with associated mechanisms. It was observed that melatonin pre-treatment applied before limited water availability significantly contrasted drought-promoted negative effects in terms of plant growth and physiological responses. Significant improvement was observed in key biological parameters like relative water content, net photosynthetic rate, stomatal conductance, transpiration rate, maximum photosynthetic efficiency of photosystem II (PSII), and PSII electron transport rate. Antioxidant apparatus was also stimulated by melatonin and enhanced activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) were noticed along with higher accumulation of proline. Gene expression studies herein revealed that melatonin promoted the up-regulation of the expression of SOD (70.7%), CAT (32.7%), and APX (66.5%) genes. As a consequence, accumulation of malondialdehyde by-products and leaf symptoms were reduced in melatonin-treated plants. All these observations offer the clear evidence that pre-treatment with melatonin ameliorate the performance of Chinese hickory plants against drought stress.

Examining hickory plantation expansion and evaluating suitability for it using multitemporal satellite imagery and ancillary data

Examining hickory plantation expansion and evaluating suitability for it using multitemporal satellite imagery and ancillary data

Mechanism of Juglone-Induced Cell Cycle Arrest and Apoptosis in Ishikawa Human Endometrial Cancer Cells.

The molecular mechanism of Juglone-induced cell cycle arrest and apoptosis in human endometrial cancer cells was investigated. Juglone was purified from the green husk of Carya cathayensis Sarg and identified by HPLC, LC-MS/MS, and NMR. At an IC50 of 20.81 μM, juglone significantly inhibited Ishikawa cell proliferation, as shown by S phase arrest mediated by inactivation of cyclin A protein ( p < 0.05). The ROS levels increased significantly after exposure to juglone, which paralleled increases in the mRNA and protein expression of p21 and decreases in the levels of CDK2, cdc25A, CHK1, and cyclin A. The expression of Bcl-2 and Bcl-xL was significantly down-regulated, whereas the expression of Bax, Bad and cyto c was up-regulated, and we later confirmed the involvement of the mitochondrial pathway in juglone-induced apoptosis. Our in vitro results stated that juglone can be studied further as an effective natural anticancer agent.