Papaya Seeds Research Articles

Green synthesis of biomass derived carbon dots via microwave-assisted method for selective detection of Fe3+ ions in an aqueous medium

Heavy metal ion represents the most serious issues to the environment and human beings. Detection and removal of heavy metal ions in the water bodies are essential. Generally, carbon dots are used as a good fluorescence sensor to sense heavy metal ions. In the present study, Papaya seeds are successfully modified into water-soluble carbon dots by one-step microwave-assisted method. Synthesised carbon dots average size was about 1.45 nm with spherical shape analysed by HRTEM and the presence of decorated functional groups was analysed by FTIR. Papaya seeds carbon dots (PPYCDs) emit bright blue colour under UV radiation. PPYCDs emits a fluorescence emission peak at 405 nm with an excitation wavelength of 335 nm. PPYCDs have the greatest quenching efficiency with Fe3+ ions and display a rapid and good sensitivity due to the decorated functional groups present on them. The Fe3+ ions detection limit was about 2.35 μMol and these ecofriendly carbon dots being synthesised by a simple procedure without any complicated instrumentation and toxic reagents, paves a way for the sensor applications effectively.

Potential Plants With Antidiabetic Activity In Glut-4 Translocation: A Narrative Review

Diabetes Mellitus (DM) is a chronic disease caused by decreased activity of the pancreas in producing insulin. Insulin resistance is characterized by decreased synthesis and translocation of GLUT-4 to cell membranes, GLUT-4 can be found in several tissues, but mainly in muscle and adipose tissue. The purpose of this study was to identify plants that have antidiabetic activity in Glut-4 translocation and further can be used as reference material in research. This research method is Article Review, by searching for articles published in 2013-2023 in the Pubmed, Google Scholar, Proquest, Springer Link, and Science Direct databases. The sample in this study was in the form of research articles that met the inclusion criteria and obtained a total of 15 articles. The results of this study showed that there was significant antidiabetic activity from the administration of Ethanol Extract on 125 mg/kg BW of Salam Leaf, 200 mg/kg BW of Areca Nut, 200 mg/kg of Papaya Seed, 96 mg/kg of Black Cumin, Combination of Sambiloto Leaf and Gotu kola leaves 300 mg/kg BW, 400 mg/kg BW of Adem Ati Bark, 100 mg/kg BW of Artemisia Leaf, 250 mg/kg BW of Coffee Beans, 200 mg/kg BW of Tapak Dara Leaves, 300 mg/kg BW of Puguntano Leaves, 50 mg/kg BW of Ginger, and 200 mg/kg BW of Cinnamon. These plant extracts have antidiabetic activity as GLUT4 translocates to the plasma membrane of muscle cells. The bioactive compounds contained in them which act as antidiabetic activity are flavonoids, saponins and tannins.

Spermicide of Papaya Seed Oil and Compounds

This study aimed to determine the spermicidal activity of papaya seed oil (Carica papaya L) from local Bali varieties against Wistar rat spermatozoa by evaluating sperm quality and the correlation between the percentage of motility and viability spermatozoa to identify its active compounds. Papaya seed oil was obtained by extracting papaya seeds using n-hexane solvent. The study was designed as follows; group of spermatozoa Wistar rat were treated with NaCl 0.9% (control, P0), with Sodium carboxy methyl cellulose (NaCMC) 0.5% (P1), with n-hexane extract 0.1%(b/v) (P2), with n-hexane extract 0.3%(b/v) (P3), and with n-hexane extract 0.5%(b/v) (P4). Column chromatography was used to separate the n-hexane extract into two fractions, A and B. The spermicide test for fractions A and B followed the same design as the test for n-hexane extract but until the P7 group. The result showed that fraction B exhibited higher spermicidal activity, with 23.3% motility and 33.8% of viability sperm at 100 ppm. The identification of fraction B by LCMS showed at least 12 mixture compounds were venoterpine, vitamin K5, 5-phenyl-2-pyridinamine, harmalan, styrylquinoline, 1,2,3,4-tetrahydro-1-benzoyl-2-methyl-4-phenylamino-quinoline, calcitroic acid, siderol, N-benzyl-linoleamide, (3β)-3-(diisopropylamino)androst-5-en-17-one, pipereicosalidine, and 25-azacholesterol. Spermicidal activity may be a synergistic effect of several compounds that have a cytotoxic activity such as styrylquinoline, 1,2,3,4-tetrahydro-1-benzoyl-2-methyl-4-phenylamino-quinoline, siderol, and pipereicosalidine.

Bio-oil production from ripe pawpaw seeds and its optimal output: Box-Behnken Design and Machine Learning approach

The purpose of this study is to use ripe pawpaw seeds for a rapid Soxhlet extractor process with n-hexane to increase the yield of the bio-oil and to make the bio-oil produced a beneficial addition to the field of bio-oil production parameter optimization and utilization for industrial applications. Thus, the produced bio-oil from kwale ripe pawpaw seeds was modeled and optimized (Ripe Carica papaya seeds) via Soxhlet extractor using Box-Behnken Design from Response Surface Methodology and Machine Learning (Python code) techniques. The result from Box-Behnken Design shows that the highest yield obtained was 23.93 wt.% at 55 g of Sample weight, 50 min of Extraction time, and 250 ml of Solvent volume while the highest value of the bio-oil yield from the Machine Learning approach is 23.97 wt.%, which is closely related to the value (23.93 wt.%) obtained from Box-Behnken Design. The R2 of the model from Box-Behnken Design was 0.9786 while the R2 from Machine Learning was 1.0. Also, the visualization in Machine Learning was more appealing than in Box-Behnken Design. Thus, Machine Learning via python coding was more reliable and effective than Box-Behnken Design in terms of prediction and accuracy of bio-oil production. Both models, however, delivered a reliable reaction under the operational conditions considered. The physicochemical characteristics of the bio-oil from ripe pawpaw seeds also meet the requirements for bio-oil. Thus, this study concluded that green biowaste oil obtained from Kwale ripe pawpaw seeds waste might be used to produce biodiesel as well as to cool spinning equipment parts.

Anticancer potential of Carica papaya Linn black seed extract against human colon cancer cell line: in vitro study

BackgroundSince cancer is one of the most prevalent diseases in the world, further studies are needed to identify the effective therapeutic modalities. The second deadliest and third most common cancer is colorectal cancer (CRC). Papaya (Carica papaya Linn) seeds offer anti-cancer properties that can cure various types of cancer, such as liver and prostate cancer.MethodsThe study aimed to evaluate the anti-cancer activity of Carica papaya seed extract on colorectal cancer cell lines (Caco-2) and used techniques to assess the anti-cancer potential. The effectiveness of SE on cell proliferation and the viability of HTB-37 Caco-2 and C-166 cells were assessed using the MTT test. Real-time RT-PCR was used to measure gene expression levels and evaluate the activity of genes involved in apoptosis, including caspase-3, p53, Cycs, and Bcl-2. Finally, flow cytometry was used to analyze apoptosis induction by detecting changes in cell morphology and DNA content.ResultsThe study showed that the MTT reduction assay was dependent on cancer cell type and concentration of SE compared to the control cells and C-166, with a mean IC50 value of 9.734 ug/ml. The cytotoxicity was accompanied by some morphological alterations in the colorectal cancer cell line (Caco-2). The expression of the genes for p53, Cycs, and caspase-3 was substantially up-regulated, while Bcl-2 was dramatically down-regulated compared to control cells. The cell cycle arrested at the G2-M phase and the presence of early and late apoptotic characteristics post-treatment increased the apoptotic profile.ConclusionIt concluded that papaya seeds aqueous extract could act as a novel therapeutic option for colorectal cancer (CRC).

EFEKTIVITAS EKSTRAK BIJI PEPAYA (Carica papaya) TERHADAP KESEMBUHAN DAN KELANGSUNGAN HIDUP IKAN JELAWAT (Leptobarbus hoevenii) YANG DIINFEKSI BAKTERI Aeromonas hydrophila

Jelawat fish (Leptobarbus hoevenii) are found in several rivers in Kalimantan and Sumatra. Often encountered obstacles in fish farming is the bacterial disease Aeromonas hydrophila, known as Motile Aeromonas Septicemia (MAS). Treatment of disease can use natural ingredients that function as antimicrobials. Papaya seeds contain antimicrobials; besides that, they also contain secondary metabolites such as phenols, terpenoids, alkaloids, and saponins. The terpenoid group is the main component of papaya seeds and has antibacterial activity. This study aims to determine the effectiveness of the best papaya seed extract on the survival of jelawat fish infected with Aeromonas hydrophila bacteria. The treatment in this study used a completely randomized design (CRD) with 4 treatments and 3 replications. The treatments were treatment A (soaking with papaya seed extract at 0 ppm), treatment B (soaking with papaya seed extract at 40 ppm), treatment C (soaking with papaya seed extract at 80 ppm), and treatment D (soaking with papaya seed extract at 120 ppm). The results showed that the administration of papaya seed extract by immersion had a significant effect on the response to high feeding, with an average absolute weight growth value of 1.33 grams, a 100% survival rate, and wound healing in sharks infected with A. hydrophila bacteria starting to normalize or heal. Meanwhile, the water quality observed during the study was quite supportive for the survival of the jelawat, with temperatures ranging from 27–28, dissolved oxygen (DO) ranging from 7-8.7 mg/L, pH ranging from 6.5 to 7.3, and ammonia values from 0.1–0.3 mg/L. Giving 80 ppm through soaking papaya seed extract with jelawat fish is the best.

Optimization of Silver Nanoparticle Biosynthesis with Papaya (Carica Papaya L) Seed Ethanol Extract and its Evaluation on Antibacterial Effects

The utilization of papaya plants (Carica papaya L) heretofore is focusing on their aril/mesocarp, leaves, and flowers, while the seeds are still neglected. Thus, this recent study attempts to make use of papaya seeds as a green-synthesis bioreductor of silver nanoparticles. It aims at finding out the characteristics of silver nanoparticles of ethanol extract of papaya seeds (AgNPs-EEPS), investigating the optimization as well as the characteristics of the AgNPs-EEPS anti-acne cream formula, and examining the antibacterial activity of Propionibacterium acnes on the AgNPs-EEPS anti-acne cream. The software design expert with factorial design method was applied to optimize the AgNPs-EEPS biosynthesis and cream formula. The analysis of the formation of AgNPs-EEPS characteristics, employing various levels of temperature and extract concentration, and applying UV- VIS spectrophotometry, resulted in a wavelength of 415 nm. Meanwhile, the implementation of particle size distribution testing, using PSD on the optimum concentration by 6% with temperature 65°C, generated a wavelength of 54 nm, pI 0.820, and zeta potential -7,7 mV. The optimization of the AgNPs-EEPS cream formula obtained optimum results at a stearic acid level 7 and cetyl alcohol 6. The characteristics of the cream produced a cream that was physically stable and met the requirements of good topical preparation. The anti-acne cream from silver nanoparticles ethanol extract of papaya seeds has the antibacterial activity of Propionibacterium acnes which belongs to the moderate category.

Thermal desorption direct analysis in real-time high-resolution mass spectrometry and machine learning allow the rapid authentication of ground black pepper and dried oregano: A proof-of-concept study.

Thermal desorption direct analysis in real-time high-resolution mass spectrometry (TD-DART-HRMS) approaches have gained popularity for fast screening of a variety of samples. With rapid volatilization of the sample at increasing temperatures outside the mass spectrometer, this technique can provide a direct readout of the sample content with no sample preparation. In this study, TD-DART-HRMS's utility for establishing spice authenticity was examined. To this aim, we directly analyzed authentic (typical) and adulterated (atypical) samples of ground black pepper and dried oregano in positive and negative ion modes. We analyzed a set of authentic ground black pepper samples (n = 14) originating from Brazil, Sri Lanka, Madagascar, Ecuador, Vietnam, Costa Rica, Indonesia, Cambodia, and adulterated samples (n = 25) consisting of mixtures of ground black pepper with this spice's nonfunctional by-products (pinheads or spent) or with different exogenous materials (olive kernel, green lentils, black mustard seeds, red beans, gypsum plaster, garlic, papaya seeds, chili, green aniseed, or coriander seeds). TD-DART-HRMS facilitated the capture of informative fingerprinting of authentic dried oregano (n = 12) originating from Albania, Turkey, and Italy and those spiked (n = 12) with increasing percentages of olive leaves, sumac, strawberry tree leaves, myrtle, and rock rose. A predictive LASSO classifier was built, after merging by low-level data fusion, the positive and negative datasets for ground black pepper. Fusing multimodal data allowed retrieval of more comprehensive information from both datasets. The resultant classifier achieved on the withheld test set accuracy, sensitivity, and specificity of 100%, 75%, and 90%, respectively. On the contrary, the sole TD-(+)DART-HRMS spectra of the oregano samples allowed construction of a LASSO classifier that predicted the adulteration of the oregano with excellent statistical indicators. This classifier achieved, on the withheld test set, 100% each for accuracy, sensitivity, and specificity.

Antihelmintic Effect of Squash Seeds (Cucurbita moschata) and Papaya Seeds (Carica papaya) in Gastrointestinal Parasites in Native Chicken (Gallus gallus domesticus).

Papaya (Carica papaya) seeds together with squash (Cucurbita moschata) seeds was pelleted to be used as dewormer, to determine its antihelmintic effects on growing chicken. They were randomly divided into four treatments and four replication with five birds per treatment was assigned using Randomized Complete Block Design (RCBD), with a total of eighty native chickens. The treatments were: Treatment A - Control (commercial dewormer) based on manufacturers recommendation; Treatment B - 75% Squash seeds and 25% Papaya seeds; Treatment C - 50% Squash seeds and 50% Papaya seeds; Treatment D - 25% Squash seeds and 75% Papaya seeds. The birds were treated orally at 2.5g of the pelleted dewormer based on the ratio of the study. In this study Treatment C, shows were highly influential in all parasites present in the chicken but of no significant result, also found to be at a lower cost.

Oil extraction from seeds of Carica papaya L.: Obtaining the lipid profile and thermal evaluation

Carica papaya L. is frequently found in northern and northeastern Brazil, the Caribbean and Central America. The papaya seed oil was extracted using Soxhlet extraction. Its oil has low acidity, iodine index, saponification and peroxide values adequate and comparable to other commercially available vegetable oils. The lipid profile of this oil was also obtained by gas chromatography; thermal characterization was performed by thermogravimetry (TG) and differential scanning calorimetry (DSC) under various heating rates. The composition of the in-nature oil indicates that oleic and palmitic fatty acids are predominant. The DSC analysis under cooling shows that the crystallization phase of this oil is similar to olive oil, and the thermogravimetric results show the thermal decomposition under nitrogen purge gas occurs in only one stage. The activation energy was obtained applying the isoconversional methods proposed by Capela and Ribeiro, Ozawa and Friedman. The obtained kinetics data leads to a dependence on the sample mass and purge gases, which results in several kinetic patterns.

Determination of mutagenic sensitivity and its manifestations on papaya (Carica papaya L.) cv. Arka Prabhath

Papaya is an important fruit crop of the family Caricaceae which needs the improvement in terms of virus resistance and shelf life with dwarf stature. Mutation breeding technique has been considered as an efficient tool adopted by plant breeders to create variability in papaya. The mutation frequency and population structure of the mutants directly depend upon the type of mutagen and the time of exposure. Irrespective of the used mutagens, the ultimate induced mutations are random and therefore determination of mutagenic sensitivity is important pre-requisite. Based on this, investigation on the induction of mutation in papaya cv. Arka Prabhath was carried out with the objective of creating genetic variability through physical mutagen. In this study, papaya seeds were irradiated with five different dose of gamma rays ranging from 50 Gy to 500 Gy. The results revealed that gradual reduction in germination, survival of seedlings and delayed germination with increase in dosage of gamma rays. Based on probit analysis, LD50 (Lethal dose) was fixed at 186.24 Gy. Total seven types of chlorophyll mutants were observed as a result of mutation. Mutagenic efficiency and effectiveness were higher in a lower dose of gamma treatment (50 Gy).

Experimental study of the drying of papaya seeds in a spouted bed and simulation of the fluid dynamics behaviour of the material

Experimental study of the drying of papaya seeds in a spouted bed and simulation of the fluid dynamics behaviour of the material

The Effect of Cocopeat Addition in Plant Media on The Quality and Quantity of Papaya Seeds (Carica papaya L.var.calina)

Papaya (Carica papaya L.) is one of the agricultural commodities that is widely cultivated by farmers in almost all parts of Indonesia. This plant is widely cultivated because varied consumption of papaya by the community makes the demand for fresh papaya fruit in the market always increase. Therefore, the cultivation of this plant is very important to be applied properly. Cocopeat is a powder which is a waste of coconut coir processing. Our previous research has shown that cocopeat is an organic material that has a positive role in the growth of patchouli seedlings. This study aimed to examine how the effect of the addition of cocopeat on the quality and quantity of growth of papaya seedlings (Carica papaya L.var.calina) on experimental land within the Universitas Negeri Manado (Unima) campus in Tondano. Experimental research was carried out with one factor, the concentration of cocopeat, with 5 levels 10, 20, 30, 40 and 50%. The control used was an organic media compounded by the analysis without using cocopeat. Each treatment and control was repeated 30 times so that the number of experimental units was 180 experimental units. Randomization was carried out by placing 10 rows and 18 columns at the Unima Patchouli Nursery Installation. The quantity parameter is the number of days the seeds germinated and the number of seeds that were successfully germinated in each treatment and control. Quality parameters include seedling height at the age of 2 months after germination (first germination); number of leaves, leaf size, wet weight and dry weight at 2 months of age. Based on the results, it can be concluded that the use of organic media with the addition of cocopeat at a concentration of 10-50% had a significant effect on leaf number, leaf size, plant height, stem diameter, wet weight, and dry weight of papaya plants in the early growth period. The addition of cocopeat concentration in the growing media will have an increasing effect on the observed parameters. Especially for the parameter of the number of days of germination after planting did not show a significant difference between all treatments.

EFEKTIVITAS SALEP EKSTRAK DAUN BAMBU, MENIRAN, BIJI PEPAYA SEBAGAI PENJINAK TUMOR DAN KANKER PAYUDARA

Bamboo plants are a source of flavonoids which are beneficial for the health of the body. To treat tumors and breast cancer, this study aims to make a synergistic ointment from the extracts of papaya seeds, meniran and bamboo leaves and a white vaseline base. Methods of data collection using experiments with accidental sampling method in the form of respondents who have had tumors and breast cancer. Data analysis used descriptive comparative analysis. Vaseline, papaya seed extract, meniran extract, and bamboo leaf extract form the composition of the bamboo leaf extract ointment. The bamboo leaf extraction process was carried out using a maceration strategy using 95% ethanol solvent. This cream-like ointment has a brown color, a pH of 6.5 and smells like bamboo leaf extract. The ointment has a spread of 3.5 cm and an adhesion time of 3.66 seconds. The content of flavonoids in the ointment was 0.1633% w/w QE and phenolics 0.371% w/w GAE. The content of cancer-preventing substances in salmon with 10 ppm is 10.09%. The ointment has an inhibitory effect on Gram-positive microorganisms of 9 mm, 10 mm and 11 mm. The results showed that there was a decrease in the size of breast tumors with a diameter of (3-5 cm) to (0.5-1 cm) with the treatment given 3 times a day for 124 days. Some respondents with stage IIA breast cancer experienced a reduction in pain and tender lumps with the treatment of giving ointment 3x a day for 17-37. The study concluded that an ointment containing meniran, papaya seeds, and bamboo leaf extract was effective against breast cancer and tumor tamer.

Optimization of Papaya Seed Oil Production Process (Carica papaya L.) with Soxhlation Extraction Method using Factorial Design

This study aims to optimize the extraction of papaya seed oil (Carica papaya L.) using a factorial design level 2. Papaya seeds are a significant component of the fruit, comprising approximately 14.3% of the total fruit weight. These seeds contain about 25% vegetable oil, predominantly composed of unsaturated fatty acids, which have the potential to be processed into consumable oil. The extraction method employed is Soxhlet extraction using a non-polar solvent, n-hexane. A factorial design level 23 was utilized to determine the most influential process variables. The variables were considered to the ratio of papaya seed mass to solvent volume (1:7 and 1:11), particle size (10 and 30 mesh), and extraction time (170 and 190 min). Optimization was conducted using the quicker method calculation, where the determination of the largest effect and the largest main effect played a crucial role. The analysis revealed that the ratio of papaya seed mass to solvent volume had the most significant main effect, with an effect value of 0.1217. From the analysis, it was found that the 1:7 ratio produced the lowest level of Free Fatty Acids (FFA) in the oil, at 0.5076%, which aligns with the Indonesian National Standard (SNI) requirement of 0.36-0.82% FFA content. Furthermore, an analysis of density, viscosity, and moisture content was conducted on the 1:7 ratio. The test results showed that the 1:7 ratio resulted in an oil density of 0.924 g/mL, moisture content of 0.07127%, and FFA content of 0.5076%. However, based on theoretical knowledge, an increase in FFA content leads to a decrease in oil quality. Additionally, higher ratios corresponded to increased moisture content and density. In conclusion, the papaya seed oil produced in this study complies with the Indonesian National Standard (SNI, 01-3555-1998) regarding FFA content (0.36-0.82%), moisture content (maximum 0.15%), and density (0.924-0.929 g/mL).

Optimization of Soxhlet Extraction Papaya Seed Oil (Carica papaya L.) with Petroleum Ether

Papaya seed oil is a high source of fatty acids, especially oleic acid, and palmitic acid. It has 71.60% oleic acid, 15.13% palmitic acid, and has a low cholesterol content so it can be useful as a food oil. This study aims to determine the effect of the ratio of ingredients, time, and particle size on the maximum extraction of papaya seed oil. Extraction of papaya seed oil was carried out by the soxhletation extraction method using petroleum ether solvent. The factorial experimental design of 23 was used to determine the significant parameters for the resulting papaya seed oil: yield, density, fatty acid content, viscosity, and water content. The most influential process variable is particle size. The most optimal papaya seed oil extraction results were obtained at a particle size of 20 mesh, an extraction time of 180 minutes, and a ratio of ingredients to the dissolution of 1:9 (35 gram500 mL). That value obtains a yield of 57.029%.

Study the Indicators of germination and growth of two varieties of papaya

The study was conducted in the Seyhan Agricultural Reserve in the city of Siba in Basra Governorate during the period (2019-2021), for the purpose of knowing the extent of the success of the cultivation of Carica papaya L. and identifying the indicators of germination and growth of two varieties of local and Red lady papaya. Papaya seeds were grown under standard germination conditions.
 The results of the study showed the superiority of the Red Lady cultivar in the percentage of germination, seed germination speed and stem diameter, while the local cultivar was superior in seedling height.

Papaya fruit by-products as novel food ingredients in cupcakes

Large quantities of waste generated from papaya fruit processing, especially seeds, can be recovered and combined to obtain added-value products. This investigation aimed to evaluate the physicochemical properties and antimicrobial activity of papaya seed powder (PSP) and papaya seed oil (PSO), and their applications as novel nontraditional food ingredients in cupcakes to promote them as high-value products. The GC–MS analysis for PSP revealed 39 compounds possess various biological activities, indicating the safety of its consumption. Papaya seeds are an excellent source of protein (27.95 %), oil (31.83 %), ash (7.86 %) and fibers (18.53 %). They have the potential to produce high-quality oleic oil with highly similar physicochemical properties and fatty acids profile as olive oil. The broad-spectrum inhibition of PSP and PSO against the tested pathogens proved their potential as natural antimicrobial agents. PSP can successfully be used (as nutritive ingredients and antimicrobial agents) in combination with wheat flour to obtain delicious and healthy nutritious chocolate cupcakes. The supplemented cupcakes (up to 15 % level) had improved quality attributes and enhanced nutritional value with respect to protein (16.89 %), ash (4.17 %) and fibers (3.28 %). PSP and PSO are safety, edible, nutritious, antimicrobial and can be processed into value-added products. They can be used as novel functional food ingredients or natural additives, providing added value to the food processing.

Study the Indicators of germination and growth of two varieties of papaya

The study was conducted in the Seyhan Agricultural Reserve in the city of Siba in Basra Governorate during the period (2019-2021), for the purpose of knowing the extent of the success of the cultivation of Carica papaya L. and identifying the indicators of germination and growth of two varieties of local and Red lady papaya. Papaya seeds were grown under standard germination conditions.The results of the study showed the superiority of the Red Lady cultivar in the percentage of germination, seed germination speed and stem diameter, while the local cultivar was superior in seedling height.

Utilization of Papaya Seeds as an Ingredient for Making Environmentally Friendly Insecticides Against Sangit Weed

Papaya (Carica Papaya) is a plant that is often found in tropical areas, one of which is in Indonesia. Inside the papaya seeds there is a karpain content, the karpain content is able to eradicate the stink bug pest that usually attacks papaya plants. This study used an experimental method in which this method tested the karpain content in papaya seeds. Making a bioinsecticide formula with a ratio of 25 ml and 75 ml; 50 ml and 50 ml and 75 ml and 25 ml. based on research and analysis of field data from the trial results showed that papaya seed extract (Carica papaya) at doses of 25%, 50%, 75%, and 100% had an effect on the death of walang sangit so that the higher the carpain content in papaya seeds, the more effective at eradicating insecticides.