The accuracy of container slot dimensions directly affects the efficiency and safety of container loading and unloading. However, traditional methods of container loading tests need to be improved for their long cycles, high costs, and low production efficiency. With the application of three-dimensional laser scanning devices in ship manufacturing, the use of simulated container loading test methods based on point cloud becomes a breakthrough in key technology for container ship construction. This paper presents a multi-objective segmentation method of cargo hold feature point cloud for container ship simulated loading tests. Data preprocessing algorithms are applied to remove noise and pseudo-image, and then a Random Sample Consensus algorithm is used to remove bulkhead and cargo bottom point clouds. Based on the density-based spatial clustering of applications with noise algorithm and the region growing algorithm, an algorithm for multiple segmentation of guide rail and bottom cone point clouds is devised, and a four-plane segmentation of guide rail point clouds is realized. Finally, a method for three-dimensional point cloud data flatness assessment and a Hausdorff distance based method for calculating the guide rail lateral and longitudinal spacing are proposed. These methods are validated through the loading test of a 16,000 TEU container ship.