Gelatin Carboxymethyl Cellulose Research Articles

Co-immobilization of lipase and β-galactosidase in gelatin-carboxymethyl cellulose blend films used as an active packaging component

β-Galactosidase and lipase were co-immobilized in polymeric blends of gelatin (GEL) and carboxymethyl cellulose (CMC) synthesized with different ratios of GEL/CMC: A (0GEL:100CMC), B (25GEL:75CMC), C (50GEL:50CMC), D (75GEL:25CMC), E (100GEL:0CMC). The films obtained good electrostatic interaction and a regular and uniform structure. The addition of CMC increased the mechanical resistance of the films, the tensile strength being 35.04 ± 0.10 MPa for A (0GEL:100CMC) films and 19.17 ± 2.44 MPa for E (100GEL:0CMC) films. Thermogravimetry (TG) showed that the addition of the enzyme decreases the thermal stability of the films. Film B (25GEL:75CMC) showed greater affinity with the added enzymes and greater thermal stability, in addition to increasing the crystallinity by up to 5.8 % and forming more transparent films. CMC was the most hydrophilic polymer, with higher moisture content (17.35 ± 1.73 %) and PVA (0.398 ± 0.047 g.mm/h.m2.kPa), higher still after the addition of enzyme (22.91 ± 1.06 % and 1.136 ± 0.087 g.mm/h.m2.kPa). The enzymes showed greater interaction with the bulky groups of CMC and the film with the highest concentration of this polymer was the one that presented the best hydrolysis rate of lactose and triglycerides, enabling the production of films.

Novel Carboxymethyl Cellulose/Gelatin-Based Film Incorporated with Zein-Stabilized Lemon Essential Oil Pickering Emulsion for the Preservation of Cherries.

In this study, a zein-stabilized lemon essential oil Pickering emulsion (ZLPE) was incorporated into a carboxymethyl cellulose/gelatin (CMC/GL) composite film to develop a bio-based packaging material with bioactive properties. The average droplet size of the ZLPE was measured at 3.62 ± 0.08 μm, with a zeta potential of -31.33 ± 0.32 mV, highlighting its excellent stability. The image results of confocal laser microscopy and scanning electron microscopy validated the uniform distribution of ZLPE in the film. The incorporation of ZLPE reduced the water solubility of films by 45.90% and decreased its water vapor permeability by 22.61%, thereby enhancing its hydrophobicity. Additionally, the ZLPE-loaded film improved mechanical properties, enhanced UV-blocking capabilities, and increased thermal stability. The introduction of ZLPE led to the antioxidant activity of the CMC/GL film increasing by six times the original level and endowed it with outstanding antibacterial properties. As a result, cherries packaged with the ZLPE film demonstrated superior preservation performance and extended shelf life in the preservation experiment, exhibiting the film's potential as a food packaging material.

Gelatin‒carboxymethyl cellulose film incorporated porphyrin metal–organic frameworks with photodynamic/photothermal synergistic antibacterial activities for pork packaging application

Gelatin‒carboxymethyl cellulose film incorporated porphyrin metal–organic frameworks with photodynamic/photothermal synergistic antibacterial activities for pork packaging application

Incorporating the Antioxidant Fullerenol into Calcium Phosphate Bone Cements Increases Cellular Osteogenesis without Compromising Physical Cement Characteristics.

Herein, fullerenol (Ful), a highly water-soluble derivative of C60 fullerene with demonstrated antioxidant activity, is incorporated into calcium phosphate cements (CPCs) to enhance their osteogenic ability. CPCs with added carboxymethyl cellulose/gelatin (CMC/Gel) are doped with biocompatible Ful particles at concentrations of 0.02, 0.04, and 0.1 wt v%-1 and evaluated for Ful-mediated mechanical performance, antioxidant activity, and in vitro cellular osteogenesis. CMC/gel cements with the highest Ful concentration decrease setting times due to increased hydrogen bonding from Ful's hydroxyl groups. In vitro studies of reactive oxygen species (ROS) scavenging with CMC/gel cements demonstrate potent antioxidant activity with Ful incorporation and cement scavenging capacity is highest for 0.02 and 0.04 wt v%-1 Ful. In vitro cytotoxicity studies reveal that 0.02 and 0.04 wt v%-1 Ful cements also protect cellular viability. Finally, increase of alkaline phosphatase (ALP) activity and expression of runt-related transcription factor 2 (Runx2) in MC3T3-E1 pre-osteoblast cells treated with low-dose Ful cements demonstrate Ful-mediated osteogenic differentiation. These results strongly indicate that the osteogenic abilities of Ful-loaded cements are correlated with their antioxidant activity levels. Overall, this study demonstrates exciting potential of Fullerenol as an antioxidant and proosteogenic additive for improving the performance of calcium phosphate cements in bone reconstruction procedures.

Enhancing the applicability of gelatin-carboxymethyl cellulose films by cold plasma modification for the preservation of fruits

Cold plasma has been induced in polymer modification, but its influence on the properties of edible coatings in fruit preservation is unclear. In the present study, cold plasma was used to improve the practicability of Gelatin-carboxymethyl cellulose (GCMC). Cold plasma modification decreased the viscosity, cohesiveness, hardness, springiness and surface microroughness of GCMC to make it easy to implement during coating. The FTIR spectrum of cold-plasma-modified GCMC (CP-GCMC) film was like GCMC film with a shift towards lower wave numbers. However, CP-GCMC film exhibited higher water vapor permeability, but lower light transmittance and water solubility than GCMC film. Moreover, CP-GCMC film-coating showed good inhibition efficiency in decay rate, weight loss, respiration rate, fruit browning, and extended shelf lives of blueberry, apple and green walnut. These results indicated that cold plasma modification did not changed chemical structure, but improved the physical, microstructural and food preservation properties of GCMC films. Hence, cold plasma modification can be used as an effective technique to enhance the application and preservation potential of edible coatings.

Carboxymethyl Cellulose/Gelatin Hydrogel Films Loaded with Zinc Oxide Nanoparticles for Sustainable Food Packaging Applications

The current research work presented the synthesis of carboxymethyl cellulose-gelatin (CMC/GEL) blend and CMC/GEL/ZnO-Nps hydrogel films which were characterized by FT-IR and XRD, and applied to antibacterial and antioxidant activities for food preservation as well as for biomedical applications. ZnO-Nps were incorporated into the carboxymethyl cellulose (CMC) and gelatin (GEL) film-forming solution by solution casting followed by sonication. Homogenous mixing of ZnO-Nps with CMC/GEL blend improved thermal stability, mechanical properties, and moisture content of the neat CMC/GEL films. Further, a significant improvement was observed in the antibacterial activity and antioxidant properties of CMC/GEL/ZnO films against two food pathogens, Staphylococcus aureus and Escherichia coli. Overall, CMC/GEL/ZnO films are eco-friendly and can be applied in sustainable food packaging materials.

Novel Polyelectrolyte Complex Membranes Containing Carboxymethyl Cellulose–Gelatin for Pervaporation Dehydration of Azeotropic Bioethanol for Biofuel

Polyelectrolyte complex membranes (PECMs) were prepared by combining sodium carboxymethyl cellulose (NaCMC) and gelatin (Ge) with variations in the Ge content in the NaCMC matrix. Characterization methods, such as infrared spectroscopy (FTIR), wide-angle X-ray diffraction (WAXD), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), contact angle analysis (CA), and universal testing machines (UTM) were used to investigate the physicochemical studies of the prepared membranes. The pervaporation characteristics of membranes with Ge content were investigated using an azeotropic mixture of water and bioethanol. The obtained data revealed that the membrane with 15 mass% of Ge (M-3) showed a maximum flux of 7.8403 × 10-2 kg/m2·h with separation selectivity of 2917 at 30 °C. In particular, the total and water flux of PECMs are shown as very close to each other indicating that the fabricated membranes could be employed to successfully break the azeotropic point of water-bioethanol mixtures. Using temperature-dependent permeation and diffusion data, the Arrhenius activation parameters were calculated, and the obtained values of water permeation (Epw) were considerably smaller than bioethanol permeation (EpE). Developed membranes showed the positive heat of sorption (ΔHs), suggesting that Henry's sorption mode is predominant.

Harvestable tumour spheroids initiated in a gelatin-carboxymethyl cellulose hydrogel for cancer targeting and imaging with fluorescent gold nanoclusters

Cancer cell spheroids are the simplest 3D in vitro cancer models and have been extensively used for cancer research. More recently, models have been becoming complex, with the introduction of a matrix and non-cancer cell types to mimic specific tumour aspects. However, applying drugs or agents in matrix-embedded cancer spheroids can be problematic. Most matrices can impede and also bind drugs or visualizing agents non-specifically, in the vicinity of the embedded spheroids. This may interfere with imaging or further analysis without breaking apart the 3D model into its constituents. Here, we developed a combined gelatin-carboxymethyl cellulose (G-CMC) hydrogel for initiating cancer spheroids that enabled intact harvesting pre/post treatment for further investigation, such as targeting and imaging. We combined CMC (1.25%) and gelatin (2.5%) at 25 °C and initiated polymerisation after autoclaving (121 °C) to obtain a mechanical strength (sheer stress) of 38 Pas versus 1.28 Pas for CMC alone. These matrix conditions facilitated separation of the spheroids from the G-CMC, using low centrifugation (100 g). We described growth of colorectal and breast cancer spheroids within the G-CMC matrix (with average diameters of 220 mm and 180 μm for representative cell lines HT29 and MCF7 at 10 days, respectively). As the cancer cells express the surface biomarker calreticulin (CRT), we manufactured anti-calreticulin IgG (anti-CRT) conjugated to fluorescent gold nanoclusters (anti-CRT-AuNC) as a probe. We harvested cancer spheroids and incubated live with the nanoclusters. Imaging demonstrated strong binding of CRT-targeted AuNCs compared to control AuNCs. This novel model preserves cancer spheroid integrity upon isolation and is well suited for targeted imaging and drug delivery of cancer in 3D.

Effect of glutraldehyde and halloysite on gelatin-carboxymethyl cellulose gel formed via phase separation: Potential application in removal of methylene blue from waste water

Interaction between gelatin A (GA) and carboxymethyl cellulose sodium salt (CMC) at a particular stoichiometric ratio i.e. concentration of GA (CG): concentration of CMC, (CCMC) =2: 0.1 (% w/v) in aqueous medium led to the phase separation phenomenon. The phase separated complexes readily form gel (G2C0.1) within 3 h as compared to gel formed by pure gelatin in 3 days. Various other gel samples G2C0.1H1 and G2C0.1GT0.5 were also formed by adding halloysite (HNT) and glutraldehyde during the formation of G2C0.1, respectively. XRD data showed that CMC, HNT and glutraldehyde were responsible for the loss of triple helix structure of gelatin in gels. Rheological measurements revealed that the gel strength of physical gel (G2C0.1) was comparable to chemically crosslinked gel (G2C0.1GT0.5) using glutraldehyde. The% swelling ratio of physical gel (G2C0.1) and chemically crosslinked gel (G2C0.1GT0.5) was observed to be 672 and 188, respectively. The gel also seems to have potential to be used for the removal of methylene blue from waste water.

Development of gelatin/carboxymethylcellulose active films containing Hass avocado peel extract and their application as a packaging for the preservation of berries

This study aimed to evaluate the effect of incorporating different concentrations (0, 200, 300, and 400 mg L−1) of avocado peel extracts (EE-AP) on the physicochemical properties and antifungal activity of gelatin-carboxymethylcellulose (Gel-CMC) films and their applicability in berry preservation. The results showed that incorporating EE-AP was compatible with the Gel-CMC film and enhanced the mechanical properties without affecting the integrity and thermal properties. The 200 mg L−1 of EE-AP concentration on films offered the best barrier properties against water vapor (2.90 × 10−11 g m−1 s−1 Pa−1). FTIR identified the intramolecular and intermolecular interactions between the functional groups of biopolymers and the EE-AP. The results obtained revealed that EE-AP incorporation into gelatin-carboxymethylcellulose films significantly decreased the moisture content (from 12.48 to 11.02%) and solubility (from 40.13 to 35.39%) of the films. All films incorporated with EE-AP showed higher colorimetric parameters and opacity than the control film (p < 0.05). The DPPH radical scavenging ability of the Gel-CMC films was increased from 24.16 to 41.12, 57.21, and 63.47%, as the extract concentration increased. Active films presented excellent ultraviolet-visible light barrier properties. The antioxidant pigments (flavonoids and chlorophylls) were estimated spectrophotometrically through absorbance. In vitro tests indicated high effectiveness to inhibit the growth of Rhizopus stolonifer and Aspergillus niger. A preservation study indicated the absence of fungal development in berries over six days of storage. In conclusion, gelatin-carboxymethylcellulose films with EE-AP represent a potential option for active packaging and can preserve fresh fruit.

Physical properties and antibacterial activity of the composited films based on carboxymethyl cellulose and gelatin functionalized with ε-polylysine

Antibacterial composite films were produced from carboxymethyl cellulose-gelatin (CMC-Gel) blend with different concentration of ε-polylysine (ε-PL) and their physical and chemical properties were characterized. Compared with the control CMC-Gel film, the functionalized films had almost indistinguishable crystalline type, thickness, tensile strength, and elongation at break, however, poor water vapor barrier properties. The results showed that the ε-PL was well incorporated into CMC-Gel matrix by electrostatic interaction, as the changes of absorption peaks in the Fourier transform infrared spectrometer and the increase of glass transition temperature in differential scanning calorimeter. The films containing ε-PL showed excellent antibacterial activity against S. aureus, B. subtilis, E. coli and P. aeruginosa. In the composting experiment, the films become degraded on the seventh day, and further degraded with the growth of molds over time. The present results showed that the active films could be a potential material for food packaging.

Electrospun carboxymethyl cellulose-gelatin nanofibrous films encapsulated with Mentha longifolia L. essential oil for active packaging of peeled giant freshwater prawn

The aims of the current study were to encapsulate Mentha longifolia L. essential oil (MEO; 0.5, 1, and 2%) into carboxymethyl cellulose-gelatin (CMC-GE) nanofibers via electrospinning and to evaluate the effects of fabricated nanofibrous films on improving the shelf-life of peeled freshwater prawns during refrigerated storage for 14 days. CMC-GE-MEO 0.5%, CMC-GE-MEO 1%, and CMC-GE-MEO 2% nanofibrous films showed significantly lower force (21.74–25.33 N), tensile strength (6.52–8.56 MPa), water vapor permeability (7.25-9.34 × 10‾4 g mm/m2 h Pa), and swelling index (20.14–27.45%) compared to the neat CMC-GE (p < 0.05). The elongation at break significantly increased when MEO was incorporated into the CMC-GE (p < 0.05). The neat CMC-GE had the highest blue-yellow index (b = 1.02 ± 0.01) and the lowest green-red index (a = −0.12 ± 0.01) and lightness (L = 95.92 ± 0.07). CMC-GE-MEO 1% and CMC-GE-MEO 2% nanofibrous films successfully improved the microbial population and chemical property of peeled prawns for 12 and 14 days, respectively. Prawns packaged with CMC-GE-MEO 2% nanofibrous film had significantly the highest sensory scores in terms of odor, color, texture, taste, and total acceptance throughout the study period (p < 0.05).

Edible films based on gelatin, carboxymethyl cellulose, and their blends as carriers of potassium salts of iso-α-acids: Structural, physicochemical and antioxidant properties

Edible films based on gelatin/carboxymethyl cellulose (GEL/CMC) blends (100/0, 75/25, 50/50, 25/75, and 0/100) incorporated with increasing concentrations (0, 15, 30, 60 mg/100g) of potassium salts of iso-α-acids (KIAA) were prepared and characterized. Mixing of GEL with CMC at 50/50 and 25/75 ratios yielded films with phase-separated microparticles. Incorporation of KIAA into the CMC100 carrier resulted in rough and opaque films. The films became more amorphous with the increasing concentration of CMC. Consequently, the higher polysaccharide content was accompanied by lower tensile strength (TS) and elastic modulus (EM). All films with the high concentration of KIAA (60 mg/100g) displayed reduced TS and EM. The control GEL-rich films exhibited significantly lower water vapor permeability (WVP) than the films with the medium- and high-levels of CMC. The KIAA-protein interactions were most likely responsible for the slight decrease in the WVP value of the GEL100 film. In general, an increase in the KIAA concentration resulted in darker yellow-green films with reduced UV/Vis transparency. As expected, the KIAA content in the films decreased over time. Probably due to the high pH, the CMC-rich films exhibited the best ability to stabilize KIAA. The CMC100 films exhibited over 10-fold weaker antiradical potential than the GEL100 films. The highest concentration of KIAA (60 mg/100g) was not an effective dose to cause a significant improvement in the antioxidant potential of all films. The antioxidant activity of the films decreased during storage.

Hybrid Gelatin/Carboxymethyl Cellulose Hydrogel Loaded Copper (II) Ion for Medical Applications

Antibacterial wound dressing has an important key in an infection in traumatic and surgical wounds. However, the antibacterial wound dressing is high cost and few domestic medical productions. The aim of this study is to prepare a wound dressing hydrogel from hybrid gelatin/carboxymethyl cellulose (Gel/CMC) hydrogel crosslinked with citric acid at different Gel: CMC ratios of 1:1, 1:2, 1:3, and 1:4 by solvent casting. The gel fractions and swelling of 6.0%w/v CuSO4 loading hybrid Gel/CMC hydrogel (Cu-Gel/CMC hydrogel) were a maximum of about 44% to 53% and 85% to 245%, respectively. The results showed that the 1:1 Gel: CMC of hydrogel produce was the most suitable condition due to its good gel fractions and swelling behavior. The cumulative Cu2+ release was a maximum of about 45% in 7 days. The hybrid Cu-Gel/CMC hydrogel showed the zone of inhibition of Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) about 16 mm and 19 mm, sequentially. The research provided that the hybrid Cu-Gel/CMC hydrogel has the potential to use in medical applications.

Structural and physical properties of carboxymethyl cellulose/gelatin films functionalized with antioxidant of bamboo leaves

In this study functional film enrichment with antioxidant of bamboo leaves (AOB) was prepared based on carboxymethyl cellulose-gelatin (CMC-Gel) mixture using casting method. Microstructures of the films were performed on atomic force microscope, Fourier transform infrared spectrometer, and X-ray diffraction (XRD). The physical properties, including the viscosity of film forming solutions, the water vapor permeability, mechanical properties, thermodynamics and transparence of CMC-Gel films were determined. The addition of AOB had not induced notable change on the surface structure features. The good compatibility among AOB, CMC and gelatin was illustrated by XRD for that the crystallization disappeared of gelatin in CMC-Gel composite film and no diffraction peaks in functionalized films. Viscosity and glass transition temperature of the film with 0.2% AOB were 779.2 ± 26.2 mPa·s and 83.24 °C, respectively, which were higher than those of all other samples. The water vapor permeation resistance and elongation at break of the films significantly decreased when the concentration of AOB exceeded 0.2%. The light barrier properties and scavenging rate of DPPH radicals were enhanced with the increasing of AOB content. Consequently, the antioxidant films utilized for food packaging could protect food quality from the destruction of heat, mechanical force, light, and oxidative free radicals.

Recent Development in Sustained Release Beads

The sustained release system is formulated to release a drug slowly over a period of time in the body. The sustained release system is having more therapeutic activity of a drug when compared to immediate release. Beads is one of the most commonly used technique in a sustained release dosage form. These are the spherical particle with size ranging from 50nm to 2mm, which contains core substances. The molecular size of the drug particle must be lesser than 1000 Dalton to formulate as SRDF. This article also contains the method of preparation by the emulsion cross-linking method. The ionic gelation process used calcium chloride as a crosslinking agent to successfully produce alginate beads. It is concluded that the release of spores and metaloxyl from the beads was controllable and sustainable, which is essential to the environment and ecosystem in the biocontrol of aflatoxin and pesticide management. The drug-like acetazolamide is prepared by using calcium alginate, and glipizide beads is coating is explained. Information about iron cross-linked carboxymethyl cellulose gelatin coacervate beads is mentioned, a brief explanation about the oyster hydrolysate containing in liposome in alginate beads is given. Then ofloxacin interpenetrating polymer beads to taste masking for sustained release information is added. The present sustained-release beads in the market is included.

New volumetric CNT-doped gelatin-cellulose scaffolds for skeletal muscle tissue engineering.

Currently, the fabrication of scaffolds for engineered skeletal muscle tissues is unable to reach the millimeter size. The main drawbacks are the poor nutrient diffusion, lack of an internal structure to align the precursor cells, and poor mechanical and electric properties. Herein, we present a combination of gelatin-carboxymethyl cellulose materials polymerised by a cryogelation process that allowed us to reach scaffold fabrication up to millimeter size and solve the main problems related to the large size muscle tissue constructs. (1) By incorporating carbon nanotubes (CNT), we can improve the electrical properties of the scaffold, thereby enhancing tissue maturation when applying an electric pulse stimulus (EPS). (2) We have fabricated an anisotropic internal three-dimensional microarchitecture with good pore distribution and highly aligned morphology to enhance the cell alignment, cell fusion and myotube formation. With this set up, we were able to generate a fully functional skeletal muscle tissue using a combination of EPS and our doped-biocomposite scaffold and obtain a mature tissue on the millimeter scale. We also characterized the pore distribution, swelling, stiffness and conductivity of the scaffold. Moreover, we proved that the cells were viable and could fuse in three-dimensional (3D) functional myotubes throughout the scaffold. In conclusion, we fabricated a biocompatible and customizable scaffold for 3D cell culture suitable for a wide range of applications such as organ-on-a-chip, drug screening, transplantation and disease modelling.

The effects of gelatin-CMC films incorporated with chitin nanofiber and Trachyspermum ammi essential oil on the shelf life characteristics of refrigerated raw beef

The effects of gelatin-carboxymethyl cellulose (Gel-CMC) based films containing chitin nano fiber (CHNF) and Trachyspermum ammi essential oil (Ajowan), on the shelf life extension of the raw beef at refrigerated temperature (4 °C) over a 12-day period were evaluated. Ajowan essential oil (AJEO) and CHNF were added to the films at 0.24, 0.64 and 1% v/v; and 2 and 4 wt%, respectively. The microbiological (total viable count, psychrotrophic count, Pseudomonas spp., Staphylococcus aureus, lactic acid bacteria, molds and yeasts), the chemical (pH, thiobarbituric acid and total volatile basic nitrogen), color and sensory properties of the packaged samples were evaluated periodically. Bacteria grew the most quickly in the control samples, followed by those wrapped with the Gel-CMC films; The lowest microbial counts, the least change in the chemical properties and the highest sensory scores after 12 days of storage were obtained for the samples wrapped in the films incorporated with 1% AJEO and 4 wt% CHNF.

A hydrogel based on dialdehyde carboxymethyl cellulose–gelatin and its utilization as a bio adsorbent

In the present study, the dialdehyde carboxymethyl cellulose (DCMC) was cross-linked covalently to gelatin via the Schiff base reaction to form a three-dimensional hydrogel (DCMC-cl-G). The crosslinking degree of DCMC and gelatin was estimated to be 50.31 ± 2.65. The maximum swelling capacity of the hydrogel in aqueous medium was around 74 g/g at pH 10.0 and 37 °C with equilibrium swelling attained in three hours and the compressive strength of the hydrogel was found to be 55 ± 0.76 kPa at 60% strain. The biodegradation studies confirmed 82.67% degradation of the hydrogel sample within a period of twelve weeks. Further, the hydrogel was evaluated as a bio adsorbent for the removal of hazardous dyes, namely Rhodamine B (RhB) and Methyl Violet (MV) from water due to its decent swelling capacity and good mechanical strength. The maximum percentage of RhB and MV removed from the respective dye solutions using DCMC-cl-G hydrogel was 96.5% and 90% at pH 6.0, respectively. Both dyes followed Langmuir adsorption isotherm, which considers monolayer adsorption of adsorbate over adsorbent, with a pseudo-second-order kinetic model. An environment friendly hybrid hydrogel of dialdehyde carboxymethyl cellulose was fabricated by crosslinking with gelatin. The use of gelatin improved the compressive strength and thermal characteristics of the hydrogel. It was utilized for the efficient removal of hazardous dyes such as Rhodamine B and Methyl violet. The biodegradation of the hydrogel was achieved up to 82.67% for a period of twelve weeks.

Synthesis, characterization and application of gelatin-carboxymethyl cellulose blend films for preservation of cherry tomatoes and grapes.

In the present study, gelatin-carboxymethyl cellulose blend film was synthesized, characterized and applied for the first time to preserve cherry tomatoes (Solanum lycopersicum var. cerasiforme) and grapes (Vitis vinifera). Gelatin (Gel) film forming solution was incorporated with carboxymethyl cellulose (CMC) at three volume per volume (Gel:CMC) ratios, namely 75:25, 50:50 and 25:75. CMC treatment has improved the transparency, tensile strength (TS), elongation at break (EAB), water vapor permeability and oxygen permeability of gelatin films. A pronounced effect was obtained for 25Gel:75CMC film. The TS and EAB values were increased from 25.98MPa and 2.34% (100Gel:0CMC) to 37.54MPa and 4.41% (25Gel:75CMC), respectively. A significant improvement in antimicrobial property of gelatin films against two food pathogens, namely Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) was obtained in the presence of CMC. The effectiveness of gelatin-CMC blend films to extend the shelf life of agricultural products was evaluated in a 14-day preservation study. The gelatin-CMC films were successfully controlled the weight loss and browning index of the fruits up to 50.41% and 31.34%, respectively. Overall, gelatin-CMC film is an environmental friendly film for food preservation.