Capsella Bursa-pastoris Research Articles

ЭМПИРИЧЕСКИЕ КРИТЕРИИ ФИТОМОНИТОРИНГА ТЕХНОГЕННОЙ НАГРУЗКИ В ДОНБАССЕ

From the data on the structural heterogeneity of indicator plants (Berteroa incana (L.) DC., Plantago major L., Reseda lutea L., Echium vulgare L., and Capsella bursa-pastoris (L.) Medik.) under unfavorable environmental conditions of Donbass selected their most informative characteristics for a general assessment of the level of anthropogenic load on local ecotopes. Empirical criteria were calculated as a result of correlation analysis between data sets on the content of heavy metals in soil samples and the values of phytoindication indices.

Біологічна ефективність гербіцидів у посівах пшениці озимої за осіннього внесення

The autumn application of herbicides in is an effective measure to control the most harmful species of winter weeds, such as Apera spica-venti, Galium aparine, Veronica persica, Consolida regalis, Capsella bursa-pastoris, Descurainia Sophia, Vicia villosa etc. These measures are especially effective in the early stages of wheat growth, starting from the seed germination stage, when it is most sensitive to the presence of weeds. The article presents the results of two-year studies on the biological effectiveness of herbicide Pledge 50, WP in different periods of development of winter wheat (BBCH 00, 11, 12, 14 and 16) alone and in a mixture with herbicides partners: Zenkor Liquid 600, SC, Marathon, SC, Granstar Gold 75, WG, Grodil Maxi 375, OD and adjuvant Skaba, EC. As a result, it was found that the biological effectiveness of the studied herbicides against Apera spica-venti and other winter and winter weeds differed significantly depending on the phase of development of winter wheat at the time of their application. Pre-ceed application of herbicides does not provide proper control of Apera spica-venti and other winter and winter weeds. For the period of spring vegetation of wheat, the control of Apera spica-venti with the use of 0.1 kg / ha of Pledge 50, WP was 69.0 %, with an overall efficiency of 68.0 %. Application of 0.4 l / ha Zenkor Liquid 600, SC provided indicators, respectively, 66.1 and 71.4 %. The application of 0.1 kg / ha of Pledge 50, WP to the BBCH 11 phase of wheat provided high control efficiency of Apera spica-venti and all weeds in general with rates of 91.2 and 87.6 %, respectively. The use of a reduced rate of 0.08 kg / ha Pledge 50, WP in the BBCH 12 phase did not reduce both the overall effectiveness against weeds – 87.4 % and Apera spica-venti, in particular – 93.1 %. The use of Pledge 50, WP (0.1 kg / ha) in the BBCH 16 phase was slightly worse, but the acceptable effect was 84.2 % against Apera spica-venti and 83.6 % overall. The best control of Apera spica-venti and all weeds in general was obtained using a mixture of Pledge 50, WP + Granstar Gold 75, WG + Skaba adjuvant, EC (0.06 kg / ha + 0.025 l / ha + 0.05 l / ha) in the phase of BBCH 14 – 97.5 and 99.2 %, which is at the level of Zenkor Liquid 600, SC + Grodil Maxi 375, OD (0.35 l / ha + 0.11 kg / ha). Significantly the highest yield of winter wheat in the experiment was obtained by application of a mixture of Pledge 50, WP + Granstar Gold 75, WG + adjuvant Skaba, EC (0.06 kg / ha + 0.025 l / ha + 0.05 l / ha) in the phase BBCH 14 – 7.56 t / ha, which is 59.8 % better than control.

ВИДОВОЙ СОСТАВ СОРНЫХ РАСТЕНИЙ В ПОСЕВАХ РАПСА В ГЕОГРАФИЧЕСКИ УДАЛЕННЫХ ДРУГ ОТ ДРУГА РЕГИОНАХ НА ПРИМЕРЕ ЛИПЕЦКОЙ И СВЕРДЛОВСКОЙ ОБЛАСТЕЙ

The aim of the study is to identify differences in the species composition of weeds in spring rapeseed crops in two geographically remote regions of the Russian Federation – Lipetsk (Central black earth region) and Sverdlovsk (Ural region). The analysis was carried out using methods of comparative Floristics and distribution of species by classes of constancy of occurrence. Differences between segetal weed flora elements in the agrophytocenoses of canola compare regions, as in floral structure, consisting of the first two "triads" of the floristic spectra and confirmed the low rate coefficient of species similarity. Species differences are represented by species that pollute rapeseed crops only in one of the compared regions. Among the species common to the agrophytocenoses of rapeseed in the two regions are such species as, Chenopodium album L., Echinochloa crusgalli (L.) Beauv., Silene praténsis (Rafn) Godr., Euphorbia helioscopia L, Fallopia convolvulus (L.) A. Loeve, Viola arvensis Murr. they are characterized by higher rates of occurrence in the Lipetsk region, and Capsella bursa-pastoris (L.) Medik., Cirsium setosum (Willd.) Bess, Erodium cicutarium (L.) L. Her., Thlaspi arvense L. – in the Sverdlovsk region. The dominant species in the Lipetsk region are Raphanus raphanistrum L., Chenopodium album L., Echinochloa crusgalli (L.) Beauv., Silene praténsis (Rafn) Godr., and in the Sverdlovsk region - Galium vaillantii DC., Stellaria media (L.) Vill., Capsella bursa-pastoris (L.) Medik., Thlaspi arvense L., Chenopodium album L., Cirsium setosum (Willd.) Bess. To protect rapeseed crops from weeds in different regions that differ in terms of heat and moisture availability, it is necessary to develop regional protection systems, preceded by mandatory monitoring studies.

Аналіз стану газонних покриттів поліфункціонального призначення на території міста Бровари

Today, there is such a significant anthropogenic impact on nature all over the world that natural compensatory processes can not to deal with its harmful effects, especially in urban areas. Therefore, it is necessary to solve the current issues of urboecology and urban greening. Lawn is a multifunctional plant formation, an integral elements of urban landscapes, the basis of compositional solutions for urban greening and the main buffer element in the city. The lawn is the most important element of landscaping in any type of greenery, which plays a sanitary role. The analysis of the state of multifunctional lawns in cities and the development its ecological and biological standards for creating stable sward in urban areas is relevant. The authors present the results of the assessment of decorative effect and quality of the structure of lawn coverings for different functional purpose in the city Brovary. We chose on the territory of Brovary different lawns for multifunctional purpose: park “Peremoha”, park named after T.G. Shevchenko, park “Sosnovyi”, cemetery “Stare”, school territory № 9, boulevard “Nezalezhnosti”, stadium “Spartak”, stadium near school № 7, stadium of Brovary Higher School of Physical Culture. Determination of turf grass and weed vegetation we carried out in accordance with the “Determinant of higher plants of Ukraine” (1987). The actual weed-infested determined by the survey method and evaluated in points. For the Latin names of plants, we use The Plant List. The assessment of lawn decorative effect (general decorativeness) carried out with the 5-point scale (Laptev, 1983). We analyzed the quality of the structure of the lawn culture phytocenosis with 6-point scale of Laptev, which consists from the number of units of shoots per area of 100 cm2. Thus, we can group the experimental lawn coverings by qualitative characteristics of the state, where 5 objects from 9 experimental (park “Sosnovyi”, cemetery “Stare”, school territory № 9, boulevard “Nezalezhnosti”, stadium of Brovary Higher School of Physical Culture) evaluated in the lowest indicators and need a complete renovation. On the territory of the cemetery “Stare” lawn we found the largest number of weed species and we noted such species as: Taraxacum officinale (L.) Weber ex FH Wigg., Plantago major L., Galinsoga parviflora Cav., Capsella bursa-pastoris L. Medik., Elytrigia repens L. (Nevski), Poa annua L., Chenopodium album L., Amaranthus retroflexus L., Fallopia convolvulus (L.) Á.Löve. Indicators of the general decorativeness of lawns in the park “Peremoha” and the stadium near the school № 7 were the same, but the results of quality analysis of the structure of lawn indicate low shoot-forming ability of turf grasses, which is essential indicator for sport lawns for formation of a resistant turf to various types of loading. The lawns on the territory of park “Peremoha”, stadium “Spartak” and stadium near school № 7 characterized by good and mediocre quality of sward and require such agronomic operations as aeration, grounding and fertilization. Sport lawns in the city Brovary characterized by high indicators of weed-infested (3 points) that in turn influences both on decorative effect and complex estimation indicators of a lawn covering. It should also be noted that the frequency of fertilization for sports lawns is higher than for other types. The research results show that the lawn on the territory boulevard “Nezalezhnosti” estimated in 3 points by general decorativeness, because the projective cover was mosaic-group (Laptev, 1983) and 2 points – the quality of the structure of the lawn. In our opinion, such low rates are probably caused by anthropogenic load, as the research object located right next to the highway, but studies of this nature need to be continued. According to the results, it is established that the condition of the lawns in Brovary for different functional purpose differs greatly in quality indicators (Laptev, 1983): sports – mediocre condition, decorative – satisfactory (except for the park “Sosnovyi”), special purpose – unsatisfactory condition. The authors propose agro-technical operations to improve the quality and decorative effect of lawns in the city. However, areas of lawn coverings with "bad" grass quality indicators require restoration.

Water extract of shepherd's purse prevents high-fructose induced-liver injury by regulating glucolipid metabolism and gut microbiota

Shepherd’s purse as a wild vegetable is getting more and more attention on health benefits. Water extract of shepherd’s purse (WESP) was prepared and analyzed for the chemical constituents. The mice were fed high-fructose (HF) diet and treated with WESP at 50, 100 and 200 mg/kg·bw for 8 weeks. The HF-fed mice receiving WESP exhibited the inhibitions against abnormal weight gain, hepatic fat accumulation and lipid metabolic by down-regulating FAS and ACC expressions. WESP also significantly alleviated hyperglycemia, oxidative stress, and inflammatory response by regulating of NF-κB pathway. Moreover, WESP dose-dependently increased the acetic, propionic, and butyric acids levels in HF-fed mice. Furthermore, WESP significantly alleviated the HF-induced gut dysbiosis by reducing the ratio of Firmicutes to Bacteroidetes and increasing the abundance of potential beneficial bacteria. Our findings indicate that WESP may be an effective dietary supplement for preventing the liver damage.

Protection of grain crops with herbicide Secator Plus, MD

The natural and climatic conditions of Belarusare favorable for development of over 300 species of weeds. Currently, perennial, wintering, winter and spring weeds dominate among grain crops. When grain crops are infested with both annual and perennial dicotyledonous weeds, herbicides containing several active substances are efficient. One of such promising herbicides is the new herbicide Secator Plus, MD(2.4 acid, 433 g/l + iodosulfuron, 62.5 g/l + amidosulfuron, 25 g/l + mefenpyr /antidote/, 62.5 g/l). This herbicide used at the rate of 0.3-0.5 l/ha for winter grain crops provided high biological efficiency against the dominant annual and some perennial dicotyledonous weeds (80–100% loss): Chenopodium album L., Capsella bursa-pastoris (L.) Medik., Galeopsis tetrahit L., Tripleurospermum inodorum Sch.-Bip., Galium aparine L., Sonchus arvensis L., etc. In the studied crops, reliable increases in preserved grain yield were obtained. Economic benefit: the net income in winter wheat crops amounted to 60.0-90.0 dollars/ha, winter triticale 35.5-43.7 dollars/ha, and spring wheat 159.9-212.3 dollars/ha. Based on the research results, the herbicide Secator Plus, MD is registered in the State Register of Plant Protection Products (pesticides) and fertilizers permitted for use on the territory of theRepublic ofBelarus. The research results are of interest in the field of herbology, since they reveal the mechanisms of interaction of various active substances in herbicide, show possibilities of its widespread use in agriculture.

DETERMINATION OF THE EFFICIENCY OF THE PROTECTION SYSTEM FOR CORE SEEDS FROM WEEDS AT VARIOUS METHODS OF TREATING SOIL UNDER CONDITIONS OF FOREST RIVER STEPPE

The article confirms the feasibility and effectiveness of modern herbicides in corn crops for various methods of tillage. According to the research results, a mixed type of weediness is formed in corn crops, among which the largest share is occupied by late spring late spring species, which accounted for 71,8 % of the total number of weeds that appeared during the growing season of the crop. These included: millet of chickens (Echinochloa crus-galli (L.) Roem.) – 16,1 %, blue bristles (Setaria glauca L.) – 30,4 %, among dicotyledons: small-flowered Galinsoga (Galinsoga rarviflora Cav. ) – 18,4 %, common thyroid (Amaranthus retroflexus L.) – 5,4 %. Early spring was 7,6 %, including white quinoa (Chenopodium album L.) – 7,5 %. Among wintering species, the shepherd's purse ordinary (Capsela bursa pastoris L. Medic) prevailed. – 3,9 %, odorless chamomile (Matricaria perforata Merat.) – 3,2 %, Field yarut (Thlaspi arvense L.) – 2,5 %, field violet (Viola arvensis Murr.) – 2,0 %, with ephemera - medium asterisk (Stellaria media L.) – 5,3 %. Perennial rootstock and weeds of sow thistle pink (Cirsium arvense (L.) Scop.) 0,9%, yellow sow thistle (Sonchus arvensis L.) – 0,1 %, field bindweed (Convolvulus arvensis L.) – 0,9 %, of perennial rhizome - creeping wheatgrass (Elytrigia repens L.) – 0.8%. An insignificant proportion of the species composition of weeds was occupied by perennial strzynekoreni and weeds. Also, when conducting research, it was found that the density of corn standing in the phase of full germination on arable land was in the range of 78,3–78,6 pcs./m2. For small disk processing, this indicator decreased to 78,1–78,2 pcs./m2. For the period of maize harvesting, differences in the density of standing of corn during various methods of tillage were also noted. So, on plowing under the conditions of herbicide protection, the density was high and was in the range of 72,9–7,2 thousand units. / Ha, while for small disk – 72,3–72,6 thousand units./ha. Herbicidal protection options ensured the death of weeds by 89–100 % within 30 days after their application. At the time of harvesting the crop, the decrease in the number of weeds compared to the untreated areas amounted to 71–98 %. The most effective was the double application of roundup max 2,4 l/ha in phases 3 and 8 of the leaves in corn. The highest yield of corn grain was obtained due to the double use of the herbicide roundup max at a rate of 2,4 l/ha. In the case of police processing, the collection was 9,4 t/ha, and for small-scale processing, it was 9,2 t/ha. Key words: corn, soil cultivation, weeds, herbicides, efficiency, productivity.

The suitability of native flowers as pollen sources for Chrysoperla lucasina (Neuroptera: Chrysopidae).

Green lacewings (Neuroptera: Chrysopidae) are key biological control agents found in a broad range of crops. Given the importance of enhancing their presence and conservation, in this study, we aim to identify and to determine the relative importance of the pollen consumed by Chrysoperla lucasina (Lacroix, 1936) from 29 pollen types offered by 51 native plant species sown in an experimental farm in Villarrubia in the south of Spain. For the purposes of this study, C. lucasina specimens were captured in the late spring of 2016 and 2017. The pollen types and other components in the alimentary canal of C. lucasina were microscopically identified using the transparency method, which is a novel technique applied to green lacewings captured in the field. The results show that (i) C. lucasina feeds on over half of the pollen types offered by the sown plant species, with no differences in behaviour by sex or year; (ii) Capsella bursa-pastoris was the most frequently identified pollen type in the alimentary canal; (iii) the majority of pollen types identified correspond to sown native plant species and not to surrounding plant species; and that (iv) most of the adults studied also consumed honeydew. Our feeding study has important implications for the selection of plant mixtures for ground cover restoration and flower vegetation strips in Mediterranean agroecosystems, which complements our previous findings on how C. lucasina use native plant species as host and reproduction sites. The plant species Capsella bursa-pastoris and Biscutella auriculata, which are best suited to provide pollen, host and reproduction sites for C. lucasina in late spring, should consequently be included in the proposed plant mixtures for Mediterranean agroecosystems.

Efficacy and safety of three plant extracts based formulations of vagitories in the treatment of vaginitis: a randomized controlled trial.

Aim There are more and more herbal preparations that are used for the purpose of treatment and improvement of the clinical manifestation of vaginitis not only by patients themselves, but also by healthcare professionals. Plant species, St. John's wort, chamomile, calendula, yarrow, shepherd's purse and tea tree oil are all well known for their anti-inflammatory, antimicrobial and wound healing activity. This paper presents the results of a clinical study in which three herbal formulations/vagitories, based on extracts of St. John's wort, chamomile, calendula, yarrow, shepherd's purse and tea tree oil, were investigated for their effectiveness on vaginitis. Methods This was a randomized controlled clinical study that included 210 women with diagnosed vaginitis. Patients were divided into two basic groups, women in reproductive period and postmenopausal period. Three subgroups including 30 patients each received one of the three vagitorie formulations for 5 days, after which the effects on subjective and objective symptoms were monitored. Results Three types of vagitories based on plant extracts had a positive effect in the treatment of vaginitis. Vagitories based on tea tree oil showed better efficiency compared to vagitories with St. John's wort and vagitories based on extracts of five plants. Women in postmenopausal group reported better tolerability of St. John's wort-based and five herbs-based vagitories compared to tea tree oil based vagitories. Conclusion Investigated vagitories showed a positive effect on both objective and subjective symptoms of vagitnis. No serious side effects were reported.

Physiological and anatomical differentiation of two sympatric weed populations.

In the vineyards of Rhineland-Palatinate (Germany), two different types of Shepherd’s Purse (Capsella bursa-pastoris) coexist: (1) the common type called ‘wild type’, and (2) the decandric type called Capsella apetala or ‘Spe’ with four stamens in place of the four petals. In this study, we compare the anatomical and physiological characters of rosette leaves of the respective types. Progeny of individual plants was cultivated in growth chambers under low- and high-light conditions. Under low-light conditions, the stomata densities of the adaxial and abaxial epidermis did not differ between the two types. When grown under high-light conditions, wild type and Spe, both exhibited increased stomata densities compared to low-light conditions, but Spe to a lesser extent than the wild type. The maximal photosynthetic capacity of Spe was lower in both, low-light and high-light conditions compared to wild-type plants. Under all CO2 concentrations, Spe seemed to be less productive. The less effective CO2 assimilation of the Spe mutant C. apetala was accompanied by later flowering. This fact prolonged the vegetative phase of Spe by about two weeks and was sufficient for the maintenance of both populations stably over years.

Shift in ecological strategy helps marginal populations of shepherd's purse (Capsella bursa-pastoris) to overcome a high genetic load.

The outcome of species range expansion depends on the interplay of demographic, environmental and genetic factors. Self-fertilizing species usually show a higher invasive ability than outcrossers but selfing and bottlenecks during colonization also lead to an increased genetic load. The relationship between genomic and phenotypic characteristics of expanding populations has, hitherto, rarely been tested experimentally. We analysed how accessions of the shepherd's purse, Capsella bursa-pastoris, from the colonization front or from the core of the natural range performed under increasing density of competitors. First, accessions from the front showed a lower fitness than those from the core. Second, for all accessions, competitor density impacted negatively both vegetative growth and fruit production. However, despite their higher genetic load and lower absolute performances, accessions from the front were less affected by competition than accessions from the core. This seems to be due to phenotypic trade-offs and a shift in phenology that allow accessions from the front to avoid competition.

Seed and soil transmission of downy mildew (Perofascia lepidii) in seed producing garden cress

Downy mildew on garden cress caused by Perofascia lepidii has become prevalent in organic seed producing areas of Germany resulting in limited seed production and high yield losses. Downy mildew occurrence in young plants in fields that have never been cultivated with garden cress raised questions about infested seeds and soils as sources of the primary inoculum and the role of other members of the plant family Brassicaceae as hosts of P. lepidii. To address these questions 31 seed lots from nine garden cress fields were examined for contamination using P. lepidii species-specific PCR assays. DNA of P. lepidii was detected in 17 seed lots out of 31. In bioassays no disease was observed on plants grown from infested seed lots but in a few plants grown from seeds. The significance of soilborne inoculum was investigated by assessing disease incidence in plants grown in soil samples. Garden cress with downy mildew symptoms were recorded when plants were grown in pathogen infested soil samples. The results show that P. lepidii is able to survive in the soil and can be a primary inoculum source for downy mildew disease. Oospores formed in infected plants underpin the role of these survival structures that get incorporated into soil after harvesting. In a host specificity-test none of the tested species Brassica juncea; Brassica napus; Brassica rapa subsp. pekinensis; Brassica rapa silvestris; Raphanus sativus var. oleiformis; Raphanus sativus var. sativus; Sinapis alba; Sinapis nigra; Arabidopsis thaliana; Capsella bursa-pastoris; Cardamine pratense; Cardaria draba; Nasturtium officinale were infected by P. lepidii.

PLANT BIODIVERSITY OF MONITORING POINTS V.AMANGELDY ALMATY REGION

The article provides a systematic analysis of the species composition of plants of the flora of v. Amangeldy, Almaty region. The comprehensive study of regional floras is becoming increasingly important in connection with the implementation of the solution to the problem of studying and preserving biological diversity. Complete information about the composition of the flora of a territory is of great theoretical importance, it allows establishing the structure and Genesis of its components, to identify individual characteristics, to restore the history of formation and trends. This ultimately is the basis of rational use of plant resources and protection of rare and endangered plants, as well as to solve many important economic problems - identifying new sources and resources of medicinal, food, fodder, ornamental and other plants. One of the main characteristics of any flora is its systematic structure, namely the ratio of families, genera and species, on the one hand, and the quantitative indicators of these taxa that determine its wealth, on the other. These indicators are components of a systematic analysis of flora in general and coenoflora in particular, the data obtained using such analysis are important material for comparative floristry. From this point of view, the systematic structure of the flora acquires the significance of one of the essential indicators that characterize the flora in the regional plan. The study of the species composition of the flora is of great importance both for understanding the history of flora and landscapes of the region as a whole, and for finding ways to conserve and use biodiversity under conditions of increasing anthropogenic pressure. Research was carried out by route-reconnaissance method in combination with a detailed study of experimental sites. In the study area – V. Amangeldy was first identified: 112 species from 88 genera and 29 families, with the dominance of the families Asteraceae (24 species or 21.4 %, 17 genera), Rosaceae (15 species or 13.39%, 11 genera), Brassicaceae (11 species, or 9.82 %, 9 genera) from Dicotyledons, and Poaceae (11 genera, 12 species, or 10.71 %) from Monocotyledonous plants. The dominant families account for 62 species, which is 55.36 % of the total number of plant species growing in this territory. Leading genera Artemisia, Potentilla and Rumex. On the territory of the study, 33 forage plant species were identified: Bromus inermis (Leyss.) Holub., Rumex confertus Willd., Trifolium pratense L., Poa bulbosa L., etc. In connection with the degradation of the vegetation cover number of weed plants was 75 species, among which are: Rumex crispus L., Capsella bursa-pastoris (L.) Medik., Cannabis ruderalis Janisch., Lathyrus tuberosus L. and other. Endemic and rare species were not found. The results of the research will serve as a basis for the rational use of the flora of Amangeldy village in Almaty region, as well as for the conservation of biodiversity. Obtained as a result of a systematic analysis of the flora of Amangeldy, it will help to identify the centers of endemism and relict, as well as to solve the issues of the place and role of this flora in a number of other adjacent floras.

Seeding and Overseeding Native Hayseed Support Plant and Soil Arthropod Communities in Agriculture Areas.

Using native seed mixtures to create or recover grassland habitats in rotation to crops or in strips surrounding fields is considered a cost-effective practice to enhance ecosystem resilience and agro-biodiversity. The aim of this research was to assess the effects of native hayseed mixtures on plant and microarthropod communities in an agricultural area of Northern Italy. Three different experimental treatments were set up. The first was a control (C) (i.e., non-seeded plots left to spontaneous vegetation succession after ploughing no deeper than 15 cm). The second, hayseed seeded (Hs) after ploughing no deeper than 15 cm. The third experimental treatment was hayseed overseeded (Ov) on the resident plant community after only a superficial harrowing. Ov plots exhibited the preeminent positive effects on the total productivity and quality of the grassland in terms of total vegetation cover, cover and richness of typical grassland species (i.e., Molinio-Arrhenatheretea species), and cover of legumes, grasses and perennial species. Moreover, Ov sites exhibited the highest abundance of microarthropod taxa and soil biological quality (QBS-ar) but only in spring, when the disturbance of ploughing negatively affected Hs and C plots. On the other hand, Hs sites showed a great reduction of invasive alien (i.e., Ambrosia artemisiifolia and Artemisia verlotiorum) and segetal weed species (i.e., Capsella bursa-pastoris and Spergula arvensis) in terms of cover. This study provides valuable indication on using hayseed mixtures to create grassland habitats as reservoir of native flora and soil biodiversity in agriculture areas.

Assembly and Analysis of the Complete Mitochondrial Genome of Capsella bursa-pastoris.

Shepherd’s purse (Capsella bursa-pastoris) is a cosmopolitan annual weed and a promising model plant for studying allopolyploidization in the evolution of angiosperms. Though plant mitochondrial genomes are a valuable source of genetic information, they are hard to assemble. At present, only the complete mitogenome of C. rubella is available out of all species of the genus Capsella. In this work, we have assembled the complete mitogenome of C. bursa-pastoris using high-precision PacBio SMRT third-generation sequencing technology. It is 287,799 bp long and contains 32 protein-coding genes, 3 rRNAs, 25 tRNAs corresponding to 15 amino acids, and 8 open reading frames (ORFs) supported by RNAseq data. Though many repeat regions have been found, none of them is longer than 1 kbp, and the most frequent structural variant originated from these repeats is present in only 4% of the mitogenome copies. The mitochondrial DNA sequence of C. bursa-pastoris differs from C. rubella, but not from C. orientalis, by two long inversions, suggesting that C. orientalis could be its maternal progenitor species. In total, 377 C to U RNA editing sites have been detected. All genes except cox1 and atp8 contain RNA editing sites, and most of them lead to non-synonymous changes of amino acids. Most of the identified RNA editing sites are identical to corresponding RNA editing sites in A. thaliana.

Bipyrazone: a new HPPD-inhibiting herbicide in wheat

Bipyrazone, 1,3-dimethyl-4-(2-(methylsulfonyl)-4-(trifluoromethyl) benzoyl)-1H-pyrazol-5-yl 1,3-dimethyl-1H-pyrazole- 4-carboxylate, is a 4-hydroxyphenylpyaunate dioxygenase (HPPD)-inhibiting herbicide. Greenhouse and field experiments were conducted to explore the potential of post-emergence (POST) application of bipyrazone in wheat fields in China. In the greenhouse study, bipyrazone at 10 and 20 g active ingredient (a.i.) ha−1 effectively controlled Descurainia sophia L., Capsella bursa-pastoris (L.) Medic., Lithospermum arvense L. and Myosoton aquaticum L. Whereas, all tested 16 wheat cultivars showed high degree of tolerance to bipyrazone at 375 and 750 g a.i. ha−1. In a dose-response experiment carried on the Shannong 6 wheat cultivar and five weed biotypes, bipyrazone was safe to the wheat cultivar, and C. bursa-pastoris, M. aquaticum and D. sophia were sensitive to this herbicide. The selectivity index (SI) between the Shannong 6 and weeds ranged from 34 to 39. The field experiments confirmed that a mixture of bipyrazone and fluroxypyr-mepthyl is practical for controlling broadleaf weeds, and bipyrazone applied alone at 30 to 40 g a.i. ha−1 can also provide satisfactory control of sensitive broadleaf weeds. These findings suggest that bipyrazone POST application has good potential for broadleaf weed management in wheat fields.

Ecological controlling weed abundance in winter wheat crops

Goal. To study the effectiveness of fertilizers and crop rotation factor in controlling weed abundance in winter wheat crops.
 Methods. Long-term field and analytical.
 Results. Research data on the effectiveness of fertilizers and legumes predecessors in controlling weed abundance in winter wheat crops are presented. It has been established that the basis of weeding of winter wheat crops in the spring tillage phase was one- and dicotyledonous species, at the time of harvest — mostly by mass dicotyledonous. In the composition of the weeds, the largest competition for winter wheat crops for light and nutrients was created by Chenopodium album — 3.4—4.3 g/m2, Capsella bursa-pastoris L. — 0.6—4.0, Spergula vulgaris — 0.3—2.9, Fallopia convolvulus — 1.0—1.1 g/ m2. The use of mineral fertilizers significantly reduced the number of weeds in winter wheat crops at the beginning of the growing season and their mass at the time of harvest. Legume predecessors had not significant effect on the dynamics of weed development in winter wheat agrocenosis.
 Conclusions. The application of mineral fertilizers has been identified as an effective measure todecreaseweeds abundance and their mass in winter wheat crops. At the fertilizer doses N60Р60К60 the number of weeds in the spring tillage phase decreased by 6.3—6.5 times as compared to the control without fertilizers, and the mass of weeds at the time of harvest — by 2.6—3.0 times. Under the clover predecessor, the mass of dicotyledonous weeds at the time of harvest compared to the spring vetch was slightly higher with the predominance of Capsella bursa-pastoris L. — 2.5 g/m2 and Chenopodium album — 2.3 g/m2. Increasing the nitrogen dose for winter wheat from 60 to 90 kg/ha only in the clover rotation chain slightly increased the weed mass at the time of harvest. The total weeds mass in comparison with the nitrogen dose of 60 kg/ha increased by 2.7 g/m2.

The phenology of an urban street flora: a transect study

Vascular plants in flower along a fixed 3.8 km route in eight streets in a primarily residential area of urban Cambridge, U.K., were recorded at monthly intervals between January 2016 and December 2019. There was a consistent annual pattern over the four years; the number of flowering species was greatest in June or July but there were still appreciable numbers of species flowering when totals were at their lowest in February or March. Five annuals (Capsella bursa-pastoris, Euphorbia peplus, Poa annua, Senecio vulgaris, Stellaria media) and one perennial (Parietaria judaica) were very frequent and flowered from January to December. Perennial species showed greater variation through the year than annual species. In most months the number of flowering British native species exceeded the combined number of archaeophytes and neophytes, but the native total peaked earlier in the summer and then declined more rapidly than that of the introductions. The transect method appeared to be effective in identifying the main annual phenological trends and also revealed the effects of extreme weather on the patterns in some seasons.

NUTRIENT AND BIOACTIVE SUBSTANCE CONTENTS OF EDIBLE PLANTS GROWN NATURALLY IN SALIPAZARI (SAMSUN)

This study was carried out for the determination of nutrient and bioactive substance contents of edible wild plants consumed as vegetables in Samsun's Salıpazarı district during September 2014 and August 2016 period. In the selected villages from identified locations, 11 species that naturally grow and are consumed as vegetables such as Alcea apterocarpa Boiss., Rumex crispus L., Urtica dioica L., Trachystemon orientalis L., Oenanthe pimpinelloides L., Smilax excelsa L., Capsella bursa-pastoris L., Aegopodium podagraria L., Arum italicum Miller, Ornithogalum sigmoideum Freyn et. Sint. and Amaranthus retroflexus L. have been identified. Protein, proline, free amino acid, superoxide dismutase (SOD) activity, lipid peroxidation level (MDA), glucose, sucrose, total soluble carbohydrate, chlorophyll, total carotenoids, β-carotene, lycopene, flavonoids, and anthocyanins contents of these plants were determined as 32.79–106.40 mg/g dry weight (DW), 5.71–47.66 µmol/g DW, 29.62–61.75 µg/g DW, 82.75–240.06 IU/mg protein, 106.36–531.05 µmol/g DW, 31.96–87.24 mg/100 g DW, 10.97–25.49 mg/100 g DW, 174.3–422.2 mg/100 g DW, 7.79–25.96 mg/100 g DW, 102.01–436.93 µg/100 g DW, 115.86–459.64 µg/100 g DW, 6.38–30.28 mg/100 g DW and 10.17–21.52 mg/100 g DW, respectively. As a result of the analyses, it was determined that there were significant differences (P < 0.01) in terms of all parameters examined among species.

Hybridization between Camelina sativa (L.) Crantz and common Brassica weeds

The movement of genes between crops and closely-related weeds has the potential to create economic and environmental problems. Camelina sativa (L.) Crantz is a promising oilseed crop and minor weed in North America, but ecological risk assessment is needed to predict crop-weed gene flow. In this study, reciprocal crosses between C. sativa cultivars and 17 Brassicaceae species in the genera Arabidopsis, Arabis, Barbarea, Camelina, Cardamine, Erysimum, and Lepidium were performed. F1 hybrids were formed between C. sativa cultivars and three species: Camelina alyssum (Mill.) Thell., Camelina microcarpa Andrz. Ex DC., and Capsella bursa-pastoris (L.) Medik. None of the other Brassica species formed hybrid offspring. F1 hybrids from C. sativa × C. alyssum showed the same pollen viability and fecundity as the parents, while hybrids from C. sativa × C. microcarpa had reduced pollen viability, silicle (pod) number and seed production. Intergeneric F1 hybrids from Camelina sativa × Capsella bursa-pastoris were sterile and this correlated with large decreases in pollen viability and number of ovules/flower. In addition, the SSR markers could be applied to identify progenies in C. sativa breeding programs as well as auxiliarily assess possibility of gene flow between C. sativa and weedy relatives. Principle coordinate analysis (PCoA) made from SSR markers clearly divided into 12 species into five groups of species. Especially, C. sativa was classified into one group with those two interfertile C. microcarpa, and C. alyssum but distinctly separated from Camelina hispida var. grandiflora (Boiss.) Hedge, Camelina laxa C. A. Mey and Camelina rumelica Velen. This division among the Camelina species was consistent with their capacity to produce fertile F1 hybrids with C. sativa. The hybridization results suggest that gene flow is likely between cultivated C. sativa and the North American weeds C. alyssum and C. microcarpa.