Canine Parvovirus Research Articles

NMR-based-Metabolomics Evaluation in Dogs Infected with Canine Parvovirus: A New Approach for Biomarker/s.

Despite aggressive treatment, canine parvovirus (CPV) enteritis remains a major cause of morbidity and mortality in puppies. Identifying reliable biomarkers of CPV enteritis is important for determining severity, length of hospital stay, and predicting clinical outcomes. This the first study that aims to emphasize the relevance of the manuscript. Forty-three (43) CPV-infected dogs were diagnosed by a rapid antigen test kit and subsequent PCR, and 10 healthy dogs were enrolled. In this prospective study, metabolomics and cardiac troponin were measured by NMR and ELISA, respectively. The diseased dogs showed statistically significant lower levels of fructose, glucose, citrate, glycerate, glutamate, carnitine, glycine, formate, and higher levels of isoleucine, isovalerate, glycolate, and creatine compared with healthy dogs. The same analysis performed on lipid parameters showed statistically significant higher levels of cholesterol variants, fatty acyl variants, free cholesterol, glycerol backbone, and sphingomyelin and lower levels of phosphoglycerates and esterified cholesterol in the diseased groups. The changes in metabolomics could be attributed to energy deficit, fat mobilization, gluconeogenesis, tricarboxylic acid cycle deficiency, and multiple organ failure. Decreased citrate, and increased fatty acyl chain-CH2CO and sphingomyelin levels will serve as the most useful biomarkers in the prognosis of dogs suffering from CPV infection.

Molecular ecology of novel amdoparvoviruses and old protoparvoviruses in Spanish wild carnivorans.

Molecular ecology of novel amdoparvoviruses and old protoparvoviruses in Spanish wild carnivorans.

Clinical Lesions in Parvo Virus Infected Puppies

Canine parvo virus (CPV) causes acute gastroenteritis in young puppies leading to profuse diarrhoea and vomiting, severe dehydration/ hypovolemia, metabolic acidosis (or alkalosis), bacterial translocation with septicaemia and endotoxemia, systemic inflammatory response syndrome (SIRS), hypercoagulability, multiorgan dysfunction, and death. CPV invades many of the internal organs such as intestinal epithelia, bone marrow, tongue, oral cavity, heart, lung, spleen, liver, kidneys and skin. In PCR positive puppies, petechial haemorrhages were observed along the tip of the eyelids, along the tip line of the lips, around the inguinal region in male puppies, around perineal region in both male and females. These lesions were focal as a single and cluster like appearance.

Urbanisation and Host Relatedness Shape Virome Composition in a Widespread, Generalist Carnivore.

Urban wildlife species have the potential to serve as links in disease transmission between wildlife, humans and domestic animals at the wildland-urban interface (WUI), contributing to both sustained cross-species transmission of pathogens and the emergence of diseases in susceptible populations. However, the relative roles of host and environmental factors in shaping the composition of pathogen communities in urban wildlife is understudied. In this study, we integrated DNA and RNA virome data with host genomic and GPS datasets to investigate factors shaping virome composition in bobcats (Lynx rufus ) at the WUI in the Tucson Mountains, Arizona, USA. Using a hybrid-capture approach for 31 scats and 17 buccal swabs, we identified multiple viruses that could affect carnivore health at the WUI, including canine parvovirus, feline astrovirus, Felis catus papillomaviruses 2 and 3 and Lyon-IARC polyomavirus. Models of virome composition and distribution of viral taxa indicated contributions of host genetic relatedness and factors relating to urbanisation (such as percentages of urban land cover, road and building densities and distances to roads). Genetic associations with virome compositions were particularly influenced by females. While females exhibit significant isolation by distance, partial Mantel tests revealed a significant correlation between beta diversity and host genetic distance in females only. To our knowledge, this study represents the first assessment of factors shaping virome composition in a wild felid. Our finding of known feline and canine pathogens in bobcats underscores the potential of the WUI to facilitate cross-species transmission between wild and domestic animals.

In vitro Evaluation of Antiviral Efficacy of Himachali Pahari Cattle Urine against Canine Parvovirus

In vitro Evaluation of Antiviral Efficacy of Himachali Pahari Cattle Urine against Canine Parvovirus

Structures and functions of the limited natural polyclonal antibody response to parvovirus infection

Host antibody responses are key components in the protection of animals against pathogens, yet the defining properties of viral antigens and induction of B cell responses that result in varied protection are still poorly understood. Parvoviruses are simple molecular structures that display 60 repeated motifs on their capsid surface, and rapidly induce strong antibody responses that protect animals from infection. We recently showed that following canine parvovirus infection of its natural host, the polyclonal response in the sera contained only two or three dominant antibodies that bound two epitopes on the capsid. Here, we characterize key antibodies present in that immune response, identifying their sequences, defining their binding properties on the capsid by cryoelectron microscopic (cryoEM) analysis, and testing their effects on viral infectivity. Two antibodies sharing the same heavy chain bound to the side of the capsid threefold spike (B-site), while another distinct antibody bound close to the threefold axis (A-site). The epitopes of these antibodies overlapped the binding site of the host receptor, the transferrin receptor type-1, but to varying degrees. The antibodies varied widely in their neutralization efficiencies as either immunoglobulins (IgGs) or monomeric antigen-binding fragments (Fabs), which was consistent with their ability to compete for the receptor. The monoclonal antibodies characterized here matched the structures from the cryoEM analysis of polyclonal sera, including those present in a different dog than the monoclonal source. This shows that after infection, a focused response to the viral antigen is produced that protects against infection.

Molecular survey of canine parvovirus type 2: the emergence of subtype 2c in New Zealand

ABSTRACT Aims To determine the genetic makeup of carnivore parvoviruses currently circulating in New Zealand; to investigate their evolutionary patterns; and to compare these viruses with those detected during the previous New Zealand-based survey (2009–2010). Methods Faecal samples from dogs (n = 40) with a clinical diagnosis of parvovirus enteritis were voluntarily submitted by veterinarians from throughout New Zealand. In addition, one sample was collected from a cat with comparable clinical presentation. The samples were used for DNA extraction and PCR amplification of viral protein 2 (VP2) of canine parvovirus type 2 (CPV-2). All samples produced amplicons of the expected sizes, which were then sequenced. The viruses were subtyped based on the presence of specific amino acids at defined locations. In addition, VP2 sequences were analysed using phylogeny and molecular network analysis. Results The majority (30/40; 75%) of CPV-2-infected dogs were younger than 6 months and 8/40 (20%) were aged between 9 months and 1 year. Most (39/41; 95%) parvoviruses were subtyped as CPV-2c, and one as the original CPV-2. The faecal sample from a cat was positive for feline panleukopenia virus. The majority (37/39; 95%) of New Zealand CPV-2c viruses were monophyletic. The remaining two New Zealand CPV-2c viruses clustered with Chinese and Sri Lankan CPV-2c viruses, separately from the main New Zealand clade. Conclusions There has been an apparent replacement of the CPV-2a viruses with CPV-2c viruses in New Zealand between 2011 and 2019. The source of the current CPV-2c viruses remains undetermined. The monophyletic nature of the majority of viruses detected most likely reflects a country-wide spread of the most successful genotype. However, an occasional introduction of CPV-2 from overseas cannot be excluded. Clinical relevance Current vaccines appear to be protective against disease caused by the CPV-2c viruses currently circulating in New Zealand. Vaccination and protection from environmental sources of CPV-2 until the development of vaccine-induced immunity remains the cornerstone of protection in young dogs against parvovirus enteritis. Ongoing monitoring of the genetic changes in CPV-2 is important, as it would allow early detection of variants that may be more likely to escape vaccine-induced immunity.

Effects of Deferred Puppy Intake on Incidence of Canine Parvovirus Infection and Survival: A Community Case Study

Canine parvovirus (CPV) threatens many canine populations, particularly those in underfunded, rural shelters, as well as the supporting organizations that transfer/transport puppies from these shelters. This study investigates the impact of a deferred puppy intake protocol on CPV incidence and outcomes among puppies transferred from a rural shelter to a supporting organization in central Ohio (Gigi’s). Rather than housing surrendered puppies within their building, the rural shelter requested that community members defer the surrender of unwanted or stray puppies. Community members then maintained possession until the next scheduled Gigi’s transport, allowing puppies to be directly placed into a clean crate and loaded onto the transport vehicle. After implementation of this protocol, CPV incidence decreased from 47% (18/38) to 9% (9/103) (P < .001). Survival rates increased but this change was not statistically significant (13/18; 72% vs. 8/9; 89%, P = .17). In general, puppies developing CPV after transport were more likely to survive than those diagnosed prior to transport (3/9; 33% vs. 18/18; 100%, P < .001). More stray puppies (20/64; 31%) than owner surrendered puppies (7/77; 9%) were diagnosed with clinical CPV (P < .001). These findings support the effectiveness of deferred intake strategies on mitigating CPV transmission and early illness identification on CPV survival.

Molecular characterization and lineage analysis of canine astrovirus strains from dogs with gastrointestinal disease in Ecuador based on ORF-2 gene analysis.

Canine Astrovirus (CaAstV) part of the Astroviridae family and genus Mamastrovirus, is a linear RNA virus with a genome of approximately 6.6 kb with three open reading frames (ORF): ORF1a and ORF1b, which code for the most conserved non-structural proteins, and ORF2, which code for the capsid protein, the most variable region of the genome. This pathogen has been linked to gastrointestinal infections, primarily causing symptoms such as vomiting, diarrhea, lethargy and severe dehydration, mainly in co-infection with other enteric viruses. In the present study, the presence of CaAstV was identified in dogs with gastrointestinal disease in Ecuador using RT-qPCR with hydrolysis probes, these samples have also tested positive for canine parvovirus type 2 (CPV-2) and canine coronavirus (CCoV). Positive samples were used for end-point RT-PCR amplification and sequencing of ORF-2 using Sanger technology. The sequences were subjected to phylogenetic analysis to determine lineages and possible recombination events. Of the 502 samples tested, 336 were found to be positive for CaAstV, 49.4% in co-infection with CPV-2, 1% in co-infection with CCoV, and 4% in simultaneous infection with all three viruses. The presence of 4 of the 5 previously reported CaAstV lineages were identified, and three possible recombinant strains were identified. Given the high frequency of CaAstV infections in dogs with gastroenteritis and its high genetic variability, it emphasizes the need to implement routine diagnostic measures that include this pathogen as one of the main causes of the disease and a risk agent in case of multiple infections.

The Detection of Mixed Infection with Canine Parvovirus, Canine Distemper Virus, and Rotavirus in Giant Pandas by Multiplex PCR.

The well-being and subsistence of giant pandas, an endangered species with a limited distribution, are currently threatened by a number of viruses, including canine parvovirus (CPV-2), canine distemper virus (CDV), and giant panda rotavirus (GPRV). To allow for timely intervention upon viral infection, it is necessary to execute rapid and accurate diagnosis of potential mixed viral infections. In the present study, we developed and validated a multiplex PCR (mPCR) approach for the detection of CPV-2, CDV, and GPRV infections. The results indicate that the method could selectively amplify the three viruses with high sensitivity and specificity, which are necessary attributes in clinical settings. Utilizing the established method, (sub)clinical giant panda samples were examined, and CPV-2, CDV, and GPRV were found in 19.72% (43 out of 218), 7.34% (16 out of 218), and 6.42% (14 out of 218) of the samples, respectively. Noticeably, mixed infections of two or three viruses were common, and this was generally observed in CDV- or GPRV-positive samples. Meanwhile, mPCR results were further validated with sequencing and the phylogenetic analysis of full-length sequences of viral genes. Taken together, our study provides an approachable assay which enables the quick detection of the three viruses mentioned above, which will benefit clinical diagnosis and laboratory epidemiological-based investigations of the giant panda population.

Introduction of a Divergent Canine Parvovirus Type 2b Strain with a Dog in Sicily, Southern Italy, Through the Mediterranean Sea Route to Europe.

Despite over four decades since its emergence, canine parvovirus type 2 (CPV-2) remains a relevant disease for dogs. Few studies, primarily only recent ones based on phylodynamic and phylogeography approaches, have highlighted the impact of rapid and long-distance transport of dogs on the CPV-2 spreading dynamics. The present study reports the genomic characterization of a CPV-2 strain detected in a dog introduced into Italy from the coasts of North Africa through the Mediterranean Sea route to Europe. The nearly complete CPV-2 sequence was obtained and analyzed. The viral isolate was characterized as a CPV-2b variant, showing genetic signatures distinct from those of CPV-2 strains detected to date in Europe. Phylodynamic and phylogeographic approaches revealed a close correlation with CPV-2 strains recently reported in the Middle East (Turkey and Egypt), which likely originated or co-evolved from Asian ones. It is at least suggestive that the inferred spreading pattern overlaps with the routes often followed by migrants travelling from Asia and Middle East to Europe, passing through Africa. This evidence for the introduction of CPV-2 via the Mediterranean Sea route to Europe highlights the relevant role of the dog movements in the global spread of emerging or re-emerging viral pathogens.

Characterization, Quantification, and Molecular Identification of Co-Infection of Canine Parvovirus (CPV-2) Variants in Dogs Affected by Gastroenteritis in Ecuador During 2022-2023.

Canine parvovirus (CPV-2) is a highly contagious virus in canines, and it is mostly spread by touching infected feces. Dogs of all ages can catch it, but puppies are more likely to suffer from it. Severe signs include vomiting, diarrhea with blood, feeling tired, and not drinking enough water. There are three different types of the original CPV-2 that have been found so far, which are CPV-2a, 2b, and 2c. The genome of CPV-2 is about 5.2 kb long and has two open reading frames (ORFs), namely the VP region and the NS region. Based on changes in amino acids at position 426, the VP2 protein distinguishes the gene apart in the VP region. Using a molecular method, this study contemplated the presence of CPV-2 and its variants in dogs that had gastroenteritis, as well as other infections. There were 511 samples tested, and 401 (78.47%) of them were positive for CPV-2. Of these, 144 (25.91%) were positive for the original genotype, 258 (64.34%) for genotype 2a, 343 (85.54%) for genotype 2b, and 167 (41.65%) for genotype 2c. Using the multiplex qPCR for genotyping, CPV-2a and CPV-2b were determined as the most frequent co-infections (16.45%). The three genotypes (2a, 2b, and 2c) were found in the samples examined based on the amino acids at position 426 of the VP2 protein, as demonstrated by the VP2 gene sequencing. Furthermore, it was discovered that in certain samples, a genetic modification at position 297 was connected to the virus's pathogenicity.

Comparative Efficacy of Conventional PCR and SYBR Green-based qPCR Assay for Detection of Canine parvovirus-2 in Diarrheic Pups from Nagpur

Comparative Efficacy of Conventional PCR and SYBR Green-based qPCR Assay for Detection of Canine parvovirus-2 in Diarrheic Pups from Nagpur

Hematological and Serum Chemistry of Canine Parvoviral Enteritis in Diverse Breeds of Dogs

Canine Parvovirus enteritis (CPV-2) is a highly infectious viral disease occurring in puppies resulting in high mortality with a myriad of clinical signs, hematological and biochemical changes during the progression of the disease. This study investigated hematological and biochemical changes in 30 CPV-positive dogs in Ibadan, Nigeria. Severe non-regenerative anemia (35.71%) and leukopenia (22 cases) were prevalent. Thrombocytopenia was severe in 73.33% of cases. Further analysis revealed normocytic hypochromic anemia in 42.86%, microcytic hypochromic anemia in 28.57%, and leukopenia categorized as mild (5), moderate (12), or severe (5). Biochemical changes included hyperproteinemia (26.7%), hyperalbuminemia, hyperglobulinemia, and elevated liver enzymes in some cases. Renal dysfunction was evident in 16.7% of dogs with elevated creatinine. Significant differences (p<0.05) were observed between infected and healthy dogs. These findings underscore the critical impact of CPV on hematological and biochemical profiles, necessitating supportive care and emphasizing the crucial role of vaccination in disease prevention.

Identification of Host-Protein Interaction Network of Canine Parvovirus Capsid Protein VP2 in F81 Cells.

Canine Parvovirus (CPV) is a highly contagious virus that causes severe hemorrhagic enteritis and myocarditis, posing a major threat to the life and health of dogs. The molecular mechanism by which VP2, the major capsid protein of CPV, infects host cells and utilizes host cell proteins for self-replication remains poorly understood. In this study, 140 host proteins specifically binding to CPV VP2 protein were identified by immunoprecipitation combined with liquid chromatography-mass spectrometry (LC-MS/MS). Subsequently, the protein Interaction Network (PPI), the annotation of gene ontology (GO) and the database of Kyoto Encyclopedia of Genes and Genomes (KEGG) were constructed for in-depth analysis. The results showed that CPV VP2 protein participated mainly in cell metabolism, cell biosynthesis, protein folding and various signal transduction processes. According to the results of proteomics analysis, we randomly selected seven proteins for co-immunoprecipitation verification, and the experimental results were consistent with the LC-MS/MS data. In addition, our study found that the expression level of the VP2-interacting protein FHL2 mediated CPV replication. Preliminary studies have shown that knockdown of FHL2 promotes CPV replication by decreasing the expression of interferon β (IFN-β) and interferon-stimulated genes (ISGs), while overexpression of FHL2 can inhibit the replication of CPV by up-regulating the expression of IFN-β and related ISGs. This study lays the foundation for elucidating the potential function of CPV VP2 protein in the process of viral infection and proliferation which provides a theoretical basis for the design of antiviral agents and vaccines.

Comparison of the sequences of the viral capsid protein 1 and viral capsid protein 2 encoded genes in symptomatic and asymptomatic cases of canine parvovirus 2 in dogs.

Canine parvovirus 2 (CPV-2) is a highly contagious virus that infects wild and domestic canines. Despite the use of a routine vaccination protocol, it is endemic in Iraq. The genetic drift of CPV-2 is a major issue worldwide because it abrogates virus control. In Iraq, there is a knowledge gap regarding the genetic sequences of asymptomatic and symptomatic CPV-2 cases. Therefore, this study aimed to perform a genetic analysis of viral capsid protein 1 (VP1) and viral capsid protein 2 (VP2), two major capsid-encoding genes, to demonstrate the possible role of certain mutations in triggering infection. Symptomatic and asymptomatic cases (n = 100/each) were tested by a polymerase chain reaction targeting VP1 and VP2 genes. The analysis revealed numerous synonymous and nonsynonymous mutations in VP1 and VP2 and in the intergenic sequence. The study identified significant genetic mutations in VP1, VP2, and the intergenic regions of CPV-2 in symptomatic and asymptomatic cases in Iraq. These mutations may contribute to the virus's ability to evade control measures such as vaccination. These findings indicate that CPV-2 polymorphisms can influence the clinical state of the disease and/or trigger infection. Understanding these genetic variations provides critical insights into CPV-2 pathogenesis and could inform improved vaccination strategies to mitigate the virus's impact in endemic regions.

Infection rate of canine parvovirus in dogs presented at private veterinary clinics in Baghdad city.

Canine parvovirus is the primary etiology of hemorrhagic diarrhea and mortality in puppies worldwide. This study was designed to investigate canine parvovirus in dogs in Baghdad by rapid testing. Rectal swabs were collected from 864 dogs presenting at sixteen private veterinary clinics with clinical signs including vomiting, anorexia, nausea, and regurgitation. All dogs were subjected to detailed clinical, physical, and laboratory investigations from early October 2021 until April 2023. A total of 127 dogs were positive for parvovirus using the rapid test. The total infection rate of CPV was 14.69%, with a higher infection rate recorded in dogs less than 6 months and 3 months of age at 44.09% and 41.73%, respectively. A significant infection rate was reported in male dogs compared with female dogs, and the Terrier breed showed a higher infection rate than the other breeds included in this study. Fever was present in 78.33% of infected dogs as well as another clinical signs related to CPV infection. The infection rate was 71.65% in vaccinated dogs and 28.35% in unvaccinated dogs. After the treatment steps, 87.4% of the infected dogs recovered, and 12.6% of the infected dogs died. CPV is circulating in dogs in Baghdad city, and unvaccinated dogs younger than 6 months were most susceptible to the virus.

<b>Infection rate of canine parvovirus in dogs presented at private veterinary clinics in Baghdad city</b>

Background: Canine parvovirus is the primary etiology of hemorrhagic diarrhea and mortality in puppies worldwide. Aim: This study was designed to investigate canine parvovirus in dogs in Baghdad by rapid testing. Methods: Rectal swabs were collected from 864 dogs presenting at sixteen private veterinary clinics with clinical signs including vomiting, anorexia, nausea, and regurgitation. All dogs were subjected to detailed clinical, physical, and laboratory investigations from early October 2021 until April 2023. Results: A total of 127 dogs were positive for parvovirus using the rapid test. The total infection rate of CPV was 14.69%, with a higher infection rate recorded in dogs less than six months and three months of age at 44.09% and 41.73%, respectively. A significant infection rate was reported in male dogs compared with female dogs, and the Terrier breed showed a higher infection rate than the other breeds included in this study. Fever was present in 78.33% of infected dogs as well as another clinical signs related to CPV infection. The infection rate was 71.65% in vaccinated dogs and 28.35% in unvaccinated dogs. After the treatment steps, 87.4% of the infected dogs recovered, and 12.6% of the infected dogs die. Conclusion: CPV is circulating in dogs in Baghdad City, and unvaccinated dogs young than 6 months were most susceptible to the virus.

Molecular epidemiology of canine parvovirus infection in dogs in Jabalpur (M.P.)

Molecular epidemiology of canine parvovirus infection in dogs in Jabalpur (M.P.)

Overview of Recent Advances in Canine Parvovirus Research: Current Status and Future Perspectives.

Canine parvovirus (CPV-2) was first identified in the late 1970s and has since become one of the most significant infectious agents affecting dogs. CPV-2 causes severe diseases such as hemorrhagic gastroenteritis and myocarditis, posing a major threat to canine health, particularly with a high mortality rate in puppies. It is globally recognized as a highly contagious and lethal pathogen. CPV is prone to rapid mutation, leading to the emergence of new variants. Despite widespread vaccination efforts, CPV remains one of the primary causes of acute gastroenteritis and death in young and juvenile dogs. Furthermore, the detection of CPV in swine populations has introduced additional challenges to its control. This review summarizes the current epidemiological status of CPV, highlighting recent advancements in diagnostic techniques and vaccine development. Additionally, it discusses the latest research on the pathogenesis of the virus and the development of antiviral agent research and proposes prevention and control suggestions for CPV under the One Health concept. In particular, there is a need to enhance surveillance of viral dynamics, accelerate the development of novel vaccines, and deepen the exploration of the underlying pathogenic mechanisms. This review aims to provide a scientific foundation for effective control of CPV and to guide future research directions.