Camera Image Acquisition Research Articles

TBC1D20 Is a Rab1 GTPase-activating Protein That Mediates Hepatitis C Virus Replication

Like other viruses, productive hepatitis C virus (HCV) infection depends on certain critical host factors. We have recently shown that an interaction between HCV nonstructural protein NS5A and a host protein, TBC1D20, is necessary for efficient HCV replication. TBC1D20 contains a TBC (Tre-2, Bub2, and Cdc16) domain present in most known Rab GTPase-activating proteins (GAPs). The latter are master regulators of vesicular membrane transport, as they control the activity of membrane-associated Rab proteins. To better understand the role of the NS5A-TBC1D20 interaction in the HCV life cycle, we used a biochemical screen to identify the TBC1D20 Rab substrate. TBC1D20 was found to be the first known GAP for Rab1, which is implicated in the regulation of anterograde traffic between the endoplasmic reticulum and the Golgi complex. Mutation of amino acids implicated in Rab GTPase activation by other TBC domain-containing GAPs abrogated the ability of TBC1D20 to activate Rab1 GTPase. Overexpression of TBC1D20 blocked the transport of exogenous vesicular stomatitis virus G protein from the endoplasmic reticulum, validating the involvement of TBC1D20 in this pathway. Rab1 depletion significantly decreased HCV RNA levels, suggesting a role for Rab1 in HCV replication. These results highlight a novel mechanism by which viruses can hijack host cell machinery and suggest an attractive model whereby the NS5A-TBC1D20 interaction may promote viral membrane-associated RNA replication.

Student Aerial Photography

Professional aerial photography missions are generally outside the reach of most students and faculty involved with teaching and research. Oblique aerial photography using handheld cameras for image acquisition from a light high‐wing aircraft offers an excellent learning experience for students in a first course in remote sensing and offers a useful research tool for graduate students and faculty engaged in environmental investigations. This paper is essentially a guide, covering all aspects of hand‐held camera aerial imaging and the subsequent processing needed to produce low‐obliques, stereograms, anaglyphs, and flight line mosaics. Scales, ground coverage distances, and stereogram and mosaic timing intervals, are included along with a section on the calculations used to produce these numbers. A list of additional resources concludes the paper.

A CCD Camera‐based Hyperspectral Imaging System for Stationary and Airborne Applications

This paper describes a CCD (charge coupled device) camera‐based hyperspectral imaging system designed for both stationary and airborne remote sensing applications. The system consists of a high performance digital CCD camera, an imaging spectrograph, an optional focal plane scanner, and a PC computer equipped with a frame grabbing board and camera utility software. The CCD camera provides 1280(h) × 1024(v) pixel resolution and true 12‐bit dynamic range. The imaging spectrograph is attached to the camera via an adapter to disperse radiation into a range of spectral bands. The effective spectral range resulting from this integration is from 457.2 nm to 921.7 nm. The optional focal plane scanner can be attached to the front of the spectrograph via another adapter for stationary image acquisition. The camera and the frame grabbing board are connected via a double coaxial cable, and the utility software allows for complete camera control and image acquisition. The imaging system captures one line image for all the bands at a time and an aircraft or the focal plane scanner serves as a mobile platform to carry out pushbroom scanning in the along‐track direction. The horizontal and vertical binning capability of the camera makes it possible to obtain images with various spatial (160, 320, 640 and 1280 pixels in image width) and spectral (32, 64, 128, 256, 512 and 1024 bands) resolutions. Formulas are presented to show the relationships among binning factors, spatial resolutions, and flight height and speed. Images with all 24 possible combinations of binning factors were collected in a laboratory setting. Airborne images with 128 bands and a width of 640 pixels were also obtained from agricultural fields, rangelands and waterways. Procedures were developed to correct geometric distortions of the airborne hyperspectral imagery. Preliminary image acquisition testing trials indicate that this CCD camera‐based hyperspectral imaging system has potential for agricultural and natural resources applications.

Measurement of in-plane motions and rotations using a simple electronic speckle pattern interferometer

We describe the construction and operation of a simple electronic speckle pattern interferometer which is sensitive to in-plane motions and rotations. The interferometer is extremely simple and easy to use. It uses a commercial digital still camera for image acquisition, and a personal computer for image storage and analysis. The interferometer was used to measure very small in-plane rotations of a rough surface, and the results were found to be in good agreement with the expected values. We propose to use this system as an instrument for the measurement of small-angle rotations.

Construction and operation of a simple electronic speckle pattern interferometer and its use in measuring microscopic deformations

The construction and operation of a simple electronic speckle pattern interferometer (ESPI) is described. The underlying theory behind the operation of the interferometer, which is sensitive to out-of-plane displacements, is given. The interferometer uses a commercial digital still camera for image acquisition and a personal computer for image storage and analysis. The interferometer was used to measure microscopic deformations of a steel plate caused by small loads. The results were found to be in good agreement with the predictions of the elastic theory. Various possible applications of the interferometer are mentioned.

Digital Fluorescence Microscopy

Fluorescence microscopy is a valuable tool for biomedical research, providing good sensitivity and high multiplicity. Specialized techniques (anisotropy measurement, resonance energy transfer) can provide information on cell spatial arrangement or label microenvironment. The unit discusses camera selection strategy, microscope hardware, and image acquisition and processing.

Gamma camera image acquisition, display, and processing with the personal microcomputer.

The authors evaluated the potential of a microcomputer for direct acquisition, display, and processing of gamma camera images. Boards for analog-to-digital conversion and image zooming were designed, constructed, and interfaced to the Macintosh II (Apple Computer, Cupertino, Calif). Software was written for processing of single, gated, and time series images. The system was connected to gamma cameras, and its performance was compared with that of dedicated nuclear medicine computers. Data could be acquired from gamma cameras at rates exceeding 200,000 counts per second, with spatial resolution exceeding intrinsic camera resolution. Clinical analysis could be rapidly performed. This system performed better than most dedicated nuclear medicine computers with respect to speed of data acquisition and spatial resolution of images while maintaining full compatibility with the standard image display, hard-copy, and networking formats. It could replace such dedicated systems in the near future as software is refined.