C-phycocyanin Production Research Articles

Potential of a novel brine-struvite-based growth medium for sustainable biomass and phycocyanin production by Arthrospira platensis.

Nutrient recovery is crucial for sustainability as it helps to recycle valuable resources, reduce environmental pollution, and promote the efficient use of natural materials in various agricultural and industrial processes. The present study investigated the impact of using brine and struvite as sustainable nutrient sources on the growth and c-phycocyanin (C-PC) production by the cyanobacterium Arthrospira platensis. Three modified growth media were compared to the standard SAG-spirul medium under yellow-white light [YLT], and blue-white light [BLT]. In the modified medium BSI, a struvite solution was utilized to replace dipotassium phosphate, while diluted brine was used to replace NaCl and de-ionized H2O. For BSII, struvite and brine were used as in BSI, with elimination of the micronutrient from the solution. In BSIII, no other nutrient sources than bicarbonate-buffer were used in addition to struvite and brine. For each medium, A. platensis was cultivated and incubated under YLT or BLT till the stationary phase. The results showed that the combinations of brine and struvite did not have any significant negative impact on the growth rates in BSIII. However, adding struvite as a phosphorus source boosted C-PC production just as effectively as YLT, with boosting biomass yield, unlike when only BLT was used. In conclusion, the brine/struvite-based media resulted in high biomass productivity with higher C-PC yields, making it an ideal growth medium for commercial sustainable C-PC production.

Application of empirical modelling to predict the growth of a marine cyanobacterium Leptolyngbyavalderiana BDU 41001 for production of bioethanol and C-phycocyanin under semi-outdoor cultivation

Marine cyanobacteria have emerged as promising feedstocks for biofuels and valuable products; however, determining optimum biomass production under dynamic natural condition is crucial for commercialisation. Implementing predictive growth models enables to comprehend the interrelation between multidimensional variables and cyanobacterial biomass production. Thus, this investigation aimed to develop a predictive growth model for marine cyanobacterium Leptolyngbyavalderiana BDU 41001 cultured for two years under semi-outdoor settings with a translucent rooftop cover to limit high sunlight and prevent rainfall, where equivalent biomass yield with the laboratory condition was recorded during summer and winter seasons, indicating thermotolerant nature of the test cyanobacterium. The Principal Component Analysis of cultivation variables showed that solar intensity, temperature, relative humidity, dissolved oxygen, and nitrate concentration impacted 97% of data variations. Subsequently, Multiple Regression Analysis with these predictors showed 0.97 predictive R2 and <0.0001 P-value, confirming the significance of the model. Notably, achieving 92–94% prediction efficiencies for biomass production of three other marine cyanobacterial species and 90% for a consortium of microalgae cultivated at the sub-arctic climate of Umeå, Sweden, highlighted the potential of the developed model. Moreover, under semi-outdoor cultivation, a 10% rise in C-phycocyanin yield and an equivalent bioethanol production as compared to the laboratory cultures unravel the potential of L.valderiana BDU 41001 as a model cyanobacterium for multifaceted pilot-scale investigations under a bio-refinery approach.

Optimization of phycobiliprotein biosynthesis in thermotolerant cyanobacteria through light parameter adjustment

This study addressed the optimization of biomass and C-phycocyanin (C-PC) concentration and its purity in the thermotolerant cyanobacterium Potamosiphon sp by adjusting key light quality parameters. Custom design and Central Composite Design experimental designs were employed, using LEDs light intensity, wavelength, and photoperiod. The results demonstrated that these lighting parameters significantly influence biomass and C-phycocyanin production, achieving up to a 100 % increase in phycocyanin accumulation and biomass production and a 50 % improvement in purity index compared to conventional white light under optimal conditions of Red:Blue light (3:1), Intensity (283 μmol m−2 s−1), and photoperiod (17.8 H light). The findings support the use of specific LEDs wavelengths that optimize the performance of production processes and the quality of products derived from cyanobacteria, such as high-value phycobiliproteins.

Digitalised prediction of blue pigment content from Spirulina platensis: Next-generation microalgae bio-molecule detection

The motive of this study is to predict the concentration of C-phycocyanin (CPC) from Spirulina platensis by adapting several colour models along with machine learning (ML) and deep learning (DL) techniques. Initially, three different culture mediums such as Zarrouk, BG-11, and AF6 were compared, and the BG-11 medium was chosen due to its overall best biomass growth, least amount of chemical usage, and CPC production. The performance of the convolutional neural network (CNN) without the input parameters of ‘Abs’ and ‘Day’ results in a higher R2 of 0.7269 as compared to both support vector machine (SVM) and artificial neural network (ANN) with R2 of 0.2725 and 0.2552, respectively. The absence of regularisation techniques has caused the scenario of model overfitting, showing results of R2Train = 0.9891 and R2Val = 0.5170 (without image augmentation) and R2Train = 0.9710 and R2Val = 0.5521 (including 20 % dropout but without image augmentation). Meanwhile, both SVM and ANN models were observed to show significantly high accuracy when including extra parameters of ‘Abs’ and ‘Day’ as compared to the CNN model with R2 of 0.9903 and 0.9827, respectively. We aim to establish a high precision and real-time assessment of microalgae biomolecule intelligent system that requires low cost, less time consumption, and is widely applicable, addressing the challenges associated with conventional microalgae quantification and identification.

Thermosynechococcus sp. CL-1 (TCL-1) as an efficient cyanobacterium in CO2 fixation, C-phycocyanin production, and removal of Cd and Pb

Photosynthetic microorganisms achieve a tremendous CO2 fixation rate and heavy metal removal. This study assessed the performance of Thermosynechococcus sp. CL-1 for CO2 fixation, heavy metals (Cd and Pb) removal, and C-phycocyanin production. The tests were conducted under various light intensities (500–2,000 µE/m2/s) and the concentration of additional Cd and Pb. Cultivation was carried out in a flat-panel photobioreactor at a temperature of 50 oC for 12 h for examination of CO2 fixation rate, heavy metal removal, and phycocyanin production. Increasing light intensity promoted TCL-1 growth performance and only a slight decline was caused by the heavy metals. Supply of 2,000 µE/m2/s light intensity with 5 MF, 2.5 mg-Cd/L, and 0.5 mg-Pb/L achieved CO2 fixation rates of 118.1±12.5, 106.9±30.1, and 114.1±26.9 mg/L/h, respectively. Total Cd removal was presented under 2,000 µE/m2/s light intensity, but only 36–67% Pb removal was achieved. Removal of heavy metals was dominated by the bio-adsorption process by the functional groups on the surface of the cells. Besides, heavy metals also reduced C-phycocyanin production by TCL-1. Thus, TCL-1 can be a potential candidate for simultaneous CO2 fixation and heavy metal removal.

Comparative Analysis of Effect of Culture Conditions on Growth and C-Phycocyanin Production in Helical and Linear Spirulina.

Spirulina (Arthrospira and Spirulina spp.) has always been characterized by the helical trichomes, despite the existence of linear forms. A great debate is now open on the morphological flexibility of Spirulina, but it seems that both trichome morphology and C-phycocyanin (C-PC) concentrations are influenced by the culture conditions.This work compared the effect of some key growth factors (medium pH as well as its carbon, potassium, and salt contents) on the growth and C-PC concentration of helical and linear Spirulina strains. Further, two-phase strategies, including light and nitrogen variation, were applied to increase the in vivo C-PC accumulation into the trichomes. Results showed that high pH induced trichomes elongation and improved growth but decreased C-PC content (+ 65 and + 43% vs. -83 and -49%, for helical and linear strains, respectively). Variations in carbon and salt concentrations negatively impacted growth and C-PC content, even if the linear strain was more robust against these fluctuations. It was also interesting to see that potassium increasing improved growth and C-PC content for both strains.The variation of light wavelength during the enrichment phase (in the two-phase strategy) improved by 50% C-PC accumulation in trichomes, especially after blue lighting for 96 h. Similar result was obtained after 48 h of nitrogen reduction, while its removal from the medium caused trichomes disintegration. The current work highlights the robustness of linear Spirulina strain and presents an efficient and scalable way to increase C-PC in vivo without affecting growth.

Importance of lighting color and period for CO2 fixation and C-phycocyanin production during Thermosynechococcus sp. CL-1 growth

Lighting arrangement is a critical factor in cultivation of photosynthetic microorganisms such as a cyanobacterium, Thermosynechococcus sp. CL-1 (TCL-1). This study observed the impact of various light intensities, light colors, and lighting periods on TCL-1 CO2 fixation rate and C-phycocyanin production. TCL-1 growth and CO2 fixation rate were not affected by the light color during continuous illumination. The best CO2 fixation rate of 2.29 ± 0.54 and 2.26 ± 0.14 g-CO2/L/d were achieved under white and red lighting, respectively. However, application of 12/12 h light-dark illumination diminished TCL-1 performance especially under red light and it might be caused by the reduction of accumulated chlorophyll-a. Furthermore, extended studies were also conducted for the efficient C-phycocyanin extraction and purification strategies, along with accumulated cyanotoxins analysis. Purification using ion-exchange chromatography elevated C-phycocyanin purity index to 3.34. Besides, treatment using distilled water and freeze dryer could remove the accumulated cylindrospermopsin and saxitoxin from TCL-1.

Cultivation of Cyanobacteria on Sustainable Dried Luffa cylindrica

Cyanobacteria are promising organisms for the sustainable production of various biotechnological interesting products. Due to their energy production via photosynthesis, the cultivation of cyanobacteria expands the CO2 cycle. Most cyanobacteria form biofilms on surfaces in their natural environment by surrounding the cells with a self-produced matrix of extracellular polymeric substances (EPS) that hold the cells together. These special growth properties need special reactors for cultivation. By immobilizing cyanobacteria on carriers, systems currently established in industry could also be used for biofilm formers. Various artificial carriers for immobilized growth of cyanobacteria and microalgae have already been described in the literature. However, the use of waste materials or natural biodegradable carriers would be more sustainable and is, therefore, the focus of this study. Dried Luffa cylindrica, zeolite, and corn stalks were investigated for their use as carriers for cyanobacteria. L. cylindrica was shown to be an excellent natural carrier for (i) Anabaena cylindrica, (ii) Nostoc muscorum 1453-12a, and (iii) Nostoc muscorum 1453-12b. Higher or at least similar growth rates were achieved when cyanobacteria were cultivated with L. cylindrica compared to submerged cultivation. Additionally, the production of EPS and C-phycocyanin was increased at least 1.4 fold in all strains by culturing on L. cylindrica. The improved growth could be explained on the one hand by the high surface area of L. cylindrica and its properties, and, on the other hand, by the release of growth-promoting nutrients from L. cylindrica to the medium.

Multifunctional Thermosynechococcus sp. CL-1 cultivation in swine wastewater for nutrients utilization, CO2 fixation, and C-phycocyanin production

BackgroundCombination of the CO2 fixation and wastewater treatment process can be simultaneously and efficiently conducted using cultivation of photosynthetic microorganisms along with generation of byproducts as the alternative benefit. MethodIn this study, a cyanobacterium, Thermosynechococcus sp. CL-1 (TCL-1), has been cultivated in unsterilized swine wastewater for assessment on nutrients (N and P) removal, CO2 fixation rate, and accumulation of C-phycocyanin. Experiment was conducted using a flat panel photobioreactor and under supply of various light intensities of 500-2,000 µE/m2/s. Significant findingsThe presence of ammonium and phosphate in the swine wastewater were favorable to support the growth of TCL-1. TCL-1 eliminated almost all the ammonium during 8 h of experimental test under illumination of higher light intensity (1,000 or 2,000 µE/m2/s). Similarly, compared to other tested light intensities, 2,000 µE/m2/s resulted in greater phosphate utilization. However, 500 µE/m2/s light intensity indicated a higher total phosphorus utilization. Besides, addition of dissolved inorganic carbon (DIC) in the swine wastewater resulted the highest CO2 fixation rate of 145.1±12.1 mg/L/h. C-phycocyanin as a valuable pigment compound was accumulated by TCL-1 with concentration of 4.9 %dwc, productivity of 6.46±0.7 mg/L/h, and in the purity index range of 0.79-0.86.

Upstream bioprocessing of Spirulina platensis microalgae using rainwater and recycle medium from post-cultivation for C-phycocyanin production

BackgroundThe cultivation of microalgae represents a water-intensive process, prompting the need to reduce its water footprint and explore alternative water sources. Therefore, this research work aims to investigate the growth of Spirulina by recycling the culture medium obtained from the post-cultivation process and substituting rainwater resources for the preparation of the culture medium. MethodsThe effect of pre-treatment methods, specifically microfiltration and UV-light was investigated. These methods were applied to treat the recycled medium and rainwater resources used for Spirulina cultivation. Biomass concentration, biomass productivity and phycocyanin accumulation of Spirulina were examined to assess the Spirulina growth under different pre-treatment conditions. Significant FindingsAfter three cultivation cycles in the recycled medium, biomass productivity decreased from 0.2246 to 0.0802 g/L/d which might be due to high salinity and biological pollutants that inhibit Spirulina growth. However, different pre-treatment methods using microfiltration and UV-light on the recycling medium were found to be effective in increasing biomass productivity from 0.1395 to 0.1861 g/L/d. Additionally, the use of rainwater for Spirulina cultivation was explored. Applying pre-treatment methods to the rainwater, biomass productivity was further improved from 0.3211 to 0.3837 g/L/d. These findings suggest that the use of pre-treatment methods and alternative rainwater resources can improve the biomass productivity of Spirulina and potentially reduce the environmental impact throughout the microalgae cultivation process.

Thermal cultivation of halophilic Cyanobacterium aponinum for C-phycocyanin production and simultaneously reducing carbon emission using wastewater

A newly halophilic Cyanobacteria aponinum PCC10605 is first explored as a robust blue alga compared to Chlorella sorokiniana (CS), Chlorella vulgaris (CV) and Chlamydomonas reinhardtii (CC400) that can be cultured at high salinity and mesophilic condition. The Logistic and Gompertz models were applied to evaluate cell growth of four algal strains under mixotrophic and autotrophic cultivation, thus the optimal condition for PCC10605 was indicated in autotrophic and 20 g/L seawater at 39°C. When nitrogen and phosphorus concentration was at the ratio of 3:1, PCC10605 grew fast and had the better C-phycocyanin (C-PC) productivity, achieving to 1.53 g/L of biomass and 0.492 g/L of C-PC under 25% light intensity and 12:12 light period. Finally, a feasible and negative carbon emission via PCC10605 which cultured in the mixed medium and wastewater at 1:2 vol ratio (i.e., M1W2), reaching to 1.227 g/L of biomass concentration, 0.503 g/L C-PC production, reducing 40.5% of chemical oxygen demand (COD), and capturing 1.71 g-CO2 simultaneously in a Photo System Instruments (PSI).

Co-cultivation of Chaetoceros calcitrans and Arthrospira platensis growing on palm oil mill effluent under outdoor condition to produce fucoxanthin and c-phycocyanin

Fucoxanthin from microalgae is promising since the productivity is higher compared to macroalgae. However, dynamic environmental and nutritional factors in large scale and outdoor condition still limiting the production. This research investigated co-cultivation of Chaetoceros calcitrans and Arthrospira platensis growing under outdoor condition and evaluated the pigment production when using palm oil mill effluent (POME) as medium growth. Results showed that co-cultivation strategy produced abundant biomass when growing up to 30 PSU salinity. The addition of POME to cultivation medium reduced fucoxanthin production, while C-phycocyanin (C-PC) production could be maintained. Mixed culture produced 27 mg/L C-PC and 5.4 mg/L fucoxanthin by using synthetic medium. The optimal condition for co-cultivation was 33% POME, 100 mg/L urea, and 17 PSU salinity to produce 1.7 mg/L fucoxanthin and 25 mg/L C-PC.

Unraveling C-phycocyanin extraction by dark incubation from marine cyanobacterium Leptolyngbya valderiana

The sustainable production of C-phycocyanin (C-PC) has accelerated the evolution from a chemical to a green paradigm. Thus, the current study investigated green C-PC extraction using a renewable source such as water as solvent from a marine cyanobacterium Leptolyngbya valderiana BDU 41001 without any chemical treatment by employing the dark incubation at room temperature (25 °C). The effects of different drying methods and standardization of two vital cultural parameters, such as variation in light intensity and salt concentration, were studied for an effective C-PC yield. Wet biomass was found to be the best for C-PC extraction (76 mg g−1) with a food-grade purity (∼0.8), which was further purified up to a reagent grade (∼2.8) using ammonium sulfate precipitation followed by dialysis. C-PC yield from L. valderiana was enhanced by ∼1.2 and ∼1.3-fold under 60 μmol photon m −2 s −1 light intensity and 8.33 g L −1 NaCl treatment, respectively, indicating the necessity for adjusting the culture condition for augmentation of C-PC accumulation. Studies on the stability of C-PC at various temperatures, pH levels, and preservatives showed that a concentration of 97% could be maintained at 5 °C for 30 days with 2% sodium chloride addition. As a final point, the cost comparison of the extraction process to the present market value of C-PC predicted that implementing such a simple green extraction procedure could generate >1500-fold higher earnings. Thus, the findings of the current study contribute towards low-cost green extraction of C-PC for establishing a feasible cyanobacterial biorefinery.

C-phycocyanin production with high antioxidant activity of a new thermotolerant freshwater Desertifilum tharense UAM-C/S02 strain

A native cyanobacterial strain, Desertifilum tharense UAM-C/S02, was studied as a possible C-phycocyanin (C-PC) producer. Photosynthetic activity (PA) assays through oxygen production determined the proper temperature and range of irradiances to be tested in a stirred tank photobioreactor. The highest C-PC productivity (97 mg L−1 d−1), with a yield of 86.46 mgC-PC gB−1 was obtained at 730 µmol photons m−2 s−1 with a biomass productivity of 608 mg L−1 d−1 and the CO2 fixation rate was 1,194 mg L−1 d−1. The 1.81 crude extract purity value is the highest reported for this genus, which was improved to biomarker-grade purity after a two-step purification strategy comprising precipitation with ammonium sulfate, followed by dialysis. The purified C-PC was almost entirely radical-free using 1 mg mL−1, which validates its potential use in therapeutic formulations.

Biorefinery Approach Applied to the Production of Food Colourants and Biostimulants from Oscillatoria sp.

In this study, a biorefinery based on Oscillatoria sp. is developed to produce high-value compounds such as C-phycocyanin, used in food colourant applications, and biostimulants, used in agriculture-related applications. First, the Oscillatoria biomass production was optimized at a pilot scale in an open raceway reactor, with biomass productivities equivalent to 52 t/ha·year being achieved using regular fertilizers as the nutrient source. The biomass produced contained 0.5% C-phycocyanins, 95% of which were obtained after freeze-thawing and extraction at pH 6.5 and ionic strength (FI) 100 mM, with a purity ratio of 0.71 achieved in the final extract. This purity ratio allows for use of the extract directly as a food colourant. Then, the extract's colourant capacity on different beverages was evaluated. The results confirm that C-phycocyanin concentrations ranging from 22 to 106 mg/L produce colours similar to commercial products, thus avoiding the need for synthetic colourants. The colour remained stable for up to 12 days. Moreover, the safety of the extracted C-phycocyanin was confirmed through toxicity tests. The waste biomass was evaluated for use as a biostimulant, with the results confirming a relevant auxin-like positive effect. Finally, an economic analysis was conducted to evaluate different scenarios. The results confirm that the production of both C-phycocyanin and biostimulants is the best scenario from an economic standpoint. Therefore, the developed biomass processing scheme provides an opportunity to expand the range of commercial applications for microalgae-related processes.

Large-scale production of Spirulina-based proteins and c-phycocyanin: A biorefinery approach

Naturally synthesized compounds have an evolving market demand as bioactive metabolites with numerous health benefits. In this context, microalgae-based bioactive compounds such as pigments and nutraceuticals are commercially available, and it is foreseen that the market demand would continue to grow due to the recent requirement for natural products in the food industry. In particular, the global market for microalgae-based proteins is foreseen to reach $ 0.84 billion by 2023, whereas the natural blue pigment (c-phycocyanin) is expected to reach a market value of $ 409.8 million by 2030. Being a protein-rich cyanobacterium (60–70% (w/w)) and a promising source for c-phycocyanin (47% of the total proteins), Spirulina has gained attention as a promising feedstock for the large-scale production of high-value proteins and c-phycocyanin. Even though Spirulina is commercially cultivated in many countries, the exclusive production of proteins and c-phycocyanin is still emerging. Besides, Spirulina-based biorefinery is a promising strategy to enhance the economic viability of the large-scale production of proteins and c-phycocyanin. In addition, utilization of waste resources in Spirulina-based biorefineries is a beneficial strategy in terms of increasing economic feasibility and environmental sustainability. The current review focuses on promoting a circular bioeconomy via the biorefining of Spirulina to produce protein and c-phycocyanin while analyzing the challenges and future perspectives associated with the overall process.

Bioprospecting of Spirulina biomass using novel extraction method for the production of C-Phycocyanin as effective food colourant

Bioprospecting of Spirulina biomass using novel extraction method for the production of C-Phycocyanin as effective food colourant

Acclimation and Characterization of Marine Cyanobacterial Strains Euryhalinema and Desertifilum for C-Phycocyanin Production.

This study involves evaluation of two native cyanobacterial strains Euryhalinema and Desertifilum isolated from a mangrove pond in Haikou (China) for their possible phycocyanin (C-PC) production. Maximal growth rate with highest chlorophyll and C-PC accumulation were observed at 28°C and 60 μmol photons m−2 s−1 photon flux density for Euryhalinema sp., while for Desertifilum sp. at 32°C and 80 μmol photons m−2 s−1. Nitrogen and iron concentration trails revealed that double strength concentration of sodium nitrate and ferric ammonium citrate in original BG11 media increased growth rate and accumulation of C-PC for both strains. Three different C-PC extraction methods were tested. The combined extraction protocol of freeze–thaw and ultrasonication markedly increased the C-PC extraction efficiency and attained the food grade purity (A 620/A 280 ratio >0.7), whereas a higher C-PC yield was found with Na-phosphate buffer. Furthermore, the clarified crude extract was used to purify C-PC by fractional ammonium sulfate [(NH₄)₂SO₄] precipitation, Sephadex G-25 gel filtration chromatography, and DEAE-sephadex ion exchange chromatography and attained analytical grade purity (A 620/A 280 ratio >3.9). Taken together, both strains showed their potential to be domesticated for valuable phycocyanin production.

Seawater-cultured Spirulina subsalsa as a more promising host for phycocyanin production than Arthrospira platensis

C-phycocyanin produced from Arthrospira platensis is of great commercial interest in healthcare industries but adoption is challenged by low quality and productivity. To find a high-quality alternative to Arthrospira platensis, this study compares C-phycocyanin production by Spirulina subsalsa and Arthrospira platensis in chemically defined freshwater medium and seawater with added monosodium glutamate residue. In the two media, Spirulina subsalsa showed higher content and first-extraction efficiency of C-phycocyanin than Arthrospira platensis did. Absorption maxima in red and blue spectral regions were observed for the first time to shift toward longer wavelengths for methanolic extracts of the cyanobacteria grown in seawater. Tightly coiled Spirulina subsalsa remained unaffected and exhibited stronger adaptation in a seawater environment than loosely spiralled Arthrospira platensis. These results preliminarily demonstrate the feasibility of cultivating Spirulina subsalsa in seawater with low-cost nutrient supplements as a promising candidate to produce C-phycocyanin as well as to reduce nutritional and freshwater investments. Overall, more research can be performed redarding culture conditions and extraction process to optimize C-phycocyanin production and extraction.

High-level production and extraction of C-phycocyanin from cyanobacteria Synechococcus sp. PCC7002 for antioxidation, antibacterial and lead adsorption

Global warming and climate change because carbon dioxide (CO2) release to atmosphere is the forecasting challenges to human being. We are facing how to overcome the dilemma on the balance between economic and environment, thus taking more efforts on green processes to meet agreement of sustainable society are urgent and crucial. The absorption of CO2 by microalgae reduces the impact of CO2 on the environment. In this study, the CO2 removal efficiency was the highest in the culture of Cyanobacterium Synechococcus sp. PCC7002 (also called blue-green algae), at 2% CO2 to reach a value of 0.86 g-CO2/g-DCW. The main product of PCC7002 is C-phycocyanin (C-PC) which regarding to phycobilisome complex in all cyanobacterial species. A 160% increasing C-PC was achieved in the cultivation under 100 μmol/m2/s light intensity, 12:12 light-period with 2% CO2 at 30 °C. The mix-culture of nitric and ammonia ions had positive effect on the cell growth and C-PC accumulation, thus realized the highest yield of 0.439 g-CPC/g-DCW. Additionally, the partial purified C-PC displayed 89% antioxidant activity of 2,2-diphenyl-1-picryhydrazyl (DPPH) and 11% of superoxide free radical scavenging activity, respectively. The production of C-PC from PCC7002 reduced the CO2 emission and exhibited antibacterial activity against Escherichia coli and lead ion adsorption at room temperature, which has the great potential for eco-friendly application.