C-glycosidic Bond Research Articles

In vitro biosynthesis of the C-glycosidic bond in aloin

Biosynthetic studies with cell-free extracts from Aloe arborescens Mill. demonstrate the transfer of the glucose moiety from UDP-glucose to aloe emodin anthrone, forming the C-glycosidic linkage in the anthracene derivative aloin. The pH-dependence and the specificity of UDP-glucose and aloe emodin anthrone for the biosynthesis of the C-glycosidic bond in aloin are shown.

Degradation and intracellular phosphorylation of showdomycin

The anti-tumor agent showdomycin is degraded in the presence of 0.05 M NH4HCO3 (pH 8.0) to a compound which carries a negatively charged group. Together with previous data (Darnall et al., 1967) it is suggested that ammonia causes a basic hydrolysis of the maleimide moiety under formation of a maleic acid-like compound. During this modification the C-glycosidic bond is not split. Intracellularly, in mouse lymphoma cells (L5178y), showdomycin is phosphorylated to showdomycintriphosphate, via the mono- and diphosphate stage.

Molecular structure and conformation of the nucleoside antibiotic derivative 2-methylformycin with a C-glycosidic bond.

Molecular structure and conformation of the nucleoside antibiotic derivative 2-methylformycin with a C-glycosidic bond.