Brevicoryne Brassicae Research Articles

Entomopathogenic fungi (EPF) are promising eco-friendly alternatives to chemical insecticides against various insect pests. We assessed the efficacy of different Beauveria bassiana (Bals.-Criv.) Vuill. strains against Brevicoryne brassicae (L.), a major pest of Brassica crops under both laboratory and field conditions. The virulence and endophytic properties of three strains of B. bassiana, the commercial strain GHA, the endemic one KA14 from the Democratic Republic of Congo (DRC), and GxABT-1 from Belgium, were assessed under laboratory conditions. Under field conditions in eastern DRC, the efficacy of the B. bassiana GHA was evaluated compared to that of a conventional insecticide. In vitro, the lethal time 50 (LT50) values ranged from 3days for B. bassiana GxABT-1 to 4days for both GHA and KA14 following direct application of fungi. Aphid exposure to fungus-treated leaves at 7 and 14days after inoculation (DAI) resulted in LT50 values of 4 to 5days for GHA and KA14. Regarding the endophytic potential, B. bassiana KA14 showed higher performance in colonizing plant tissues and improving plant height growth. In the dual-choice tests, aphids were initially attracted to fungus-treated plants at 7 DAI but showed no preference between treated and control plants at 14 DAI. Field trials indicated no significant difference between fungal and chemical insecticide treatments, but both significantly reduced B. brassicae infestation compared to the Control. Our results support the interest of exploring the potential of the endemic B. bassiana KA14 strain against various insect pests and applying different methods.

This research reported a novel and sustainable green synthesis of Ag/Fe2O3 and Ag/Co NCs using Anabasis articulata extract. To the best of our knowledge, this is the first report of using A. articulata extract as a reducing and stabilizing dual agent in nanocomposite synthesis, which provides an eco-friendly alternative to traditional chemical or physical methods. This approach prevents the use of toxic reagents, minimizes energy requirements, and leverages the phytochemical-dense matrix of the extract to maximize the bioactivity of the resulting nanocomposites. The volatile components of A. articulata extract were analyzed by GC-MS analysis. The nanocomposites were characterized by FTIR, UV-Vis, XRD, SEM, EDX, HR-TEM, and zeta potential analysis. The phytochemical analysis of A. articulata extract revealed high amounts of phytocomponents (phenolic: 134.13 ± 1.73 mg GAE per g DS), which significantly decreased during the synthesis of nanocomposites. The antioxidant activity tests revealed potent results with IC50 at 0.057 ± 1.23 mg mL−1 for plant extract, Ag/Fe2O3 NC (IC50 = 0.09 ± 1.02 mg mL−1), and Ag/Co NC (IC50 = 0.2 ± 1.02 mg mL−1) using DPPH assay, which are in agreement with the results of the FRAP assay. Remarkably, Ag/Fe2O3 NC showed the most noteworthy antibacterial activity, against all types of pathogenic bacteria (19–24 mm). Both Aphis craccivora and Brevicoryne brassicae exhibited dose-dependent mortality under all treatments, with Ag/Co NC achieving the lowest LC50 and highest toxicity index values, slightly surpassing azadirachtin and markedly outperforming Ag/Fe2O3 NC and the A. articulata extract. These findings demonstrate the promise of A. articulate-mediated Ag/Fe2O3 and Ag/Co NCs as potent and promising green nanomaterials, representing the first use of their dual antimicrobial–insecticidal application and underscoring their potential in sustainable pest management strategies.

Background and aim Datura metel L. is a medicinal plant with the documented bioactivities, but its phytochemical profile and dual antioxidant/insecticidal potential remain underexplored, particularly regarding solvent extraction efficacy. This study aimed to (1) characterize the chemical composition of D. metel leaf extracts using ethanol, ethyl acetate, dichloromethane, hexane, and distilled water; (2) evaluate their antioxidant activity via DPPH assay; and (3) assess insecticidal effects against cabbage aphid (Brevicoryne brassicae). Experimental approach Phytochemical screening was conducted to identify alkaloids, flavonoids, phenolic, and glycosides. Gas chromatography-mass spectrometry (GC-MS) analysis characterized the major compounds, while antioxidant activity was measured via DPPH free radical-scavenging assay. Insecticidal efficacy was tested using residual and contact bioassays at varying concentrations (12.5, 25, 50, and 200μgmL−1). Key findings GC-MS identified four major compounds, including 11H-pyrido[3′,2’:4,5]imidazo[2,1-b][1,3]benzothiazin-11-one (29.76%), a heterocyclic compound with the reported insecticidal/anti-inflammatory properties, and didodecanol phthalate (11.73%), a plasticizer with antimicrobial activity. The aqueous extract showed the highest phenolic content (75.03 mg GAE g−1), while ethyl acetate yielded the highest flavonoids (99.33 mg QE g−1). All extracts exhibited potent antioxidant activity (DPPH inhibition: 63.91–81.59%). Insecticidal assays revealed dose-dependent aphid mortality, peaking at 81.66% (contact) and 73.33% (residual) at 200 mg mL−1 after 48h. Conclusions The study highlights D. metel’s dual bioactivity, bridging a critical gap in solvent-specific efficacy for agrochemical and pharmaceutical applications.

Cabbage (Brassica oleracea L.) the second most important vegetable crop in Ethiopia, but cabbage aphid (Brevicoryne brassicae L.) is the main challenge for its production. The aim of the present study was, therefore, to investigate insecticidal potential aqueous extracts of Melia azedarach, Allium sativum and their combination at 5% and 10%, and 15%w/v against cabbage aphid under laboratory and field condition. Dimethoate 40% EC and distilled water were used as a positive and negative control respectively. Mortality was observed, counted after 24, 48 and 72 h exposure both in the laboratory and field conditions. The result showed that the extracts of almost all botanicals at 15% w/v concentration caused the highest (100%) toxicity effect at the end of three days. In conclusion, based on this study M. azedarach, A. sativum aqueous extracts could serve as cabbage crop protectant from can cabbage aphid (Brevicoryne brassicae for smallholder farmers.

Nineteen Brassica accessions from four C-genome species were screened under controlled environment conditions for their effects on the development of the cabbage aphid, Brevicoryne brassicae. The aim was to identify accessions with antibiosis host plant resistance (HPR) to B. brassicae. Candidate Brassica accessions were selected because of (i) upregulation of transcription factors gene orthologues acting within the jasmonic acid signalling pathway, or (ii) having been reported in the 1990s as partially resistant to cabbage aphid. Accessions were evaluated by placing three 1-day-old aphid nymphs on six true leaf stage plants and recording the aphid population size 14 days later. There was significant (GLM, P < 0.05) variation in aphid population size between the accessions. Aphid populations on the most resistant accessions were approximately one third the size of those on the most susceptible accessions. Two accessions (B. cretica, B. villosa) supported significantly fewer aphids compared to a commercial variety of B. oleracea used as a reference control. Eight accessions (including B. cretica, B. oleracea and B. villosa) were selected for further study of varietal effects upon aphid biology. Significant differences in B. brassicae reproduction, intrinsic rate of increase, and population doubling time were identified between these eight accessions. Linear regression analysis identified that HPR was explained mainly by varietal effects on aphid pre-reproductive period, which accounted for 53.9% of the variation in population development resistance screening results. Partial HPR could be a valuable trait for plant breeding to limit aphid population development as part of an integrated pest management strategy. © 2025 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Relevance. Northern territories are most suitable for virus-free potato seed production due to the low infection background and weak activity of aphids that carry viral infections. The aphidofauna of the Arkhangelsk region has been poorly studied. This work is a generalization of the results of many years of catching winged aphids on potato plantings.Methods. Winged aphids were caught with yellow water traps and identified using insect identifiers.Results. During the research period, 43 species of aphids were identified, with populations in different years ranging from 61 to 1778 individuals. Of these, 12 species have the ability to spread potato viruses: Aсyrthosiphon pisum Harr., Aphis fabae Scop., Aphis nasturtii Kalt., Aulocorthum solani Kalt., Brevicoryne brassicae L., Cavariella aegopodii Scop., Hyperomyzus lactucae L., Lipaphis erysimi Kalt., Macrosiphum euphorbiae Thomas, Metopolophium dirhodum Walk., Rhopalosiphum padi L. и Sitobion avenae F. Aphid vectors of potato viruses in the annual population structure accounted for 62–98%, the most numerous representatives of the species A. fabae Scop., A. nasturtii Kalt., H. lactucae L., Rh. padi L. The obtained data allowed us to assess the region by the prevalence of aphid vectors of viral infection, which ranged from low (14–22 individuals per year per 1 trap) to medium (415 insects per 1 trap per year). Winged aphids visited plantings throughout the entire period of potato vegetation, therefore, in the conditions of the northern part of the Arkhangelsk region, it is necessary to carry out measures to protect potato plants.

Cabbage aphid Brevicoryne brassicae (Linnaeus) and mustard aphid Lipaphis erysimi (Kaltenbach) are economic pests of major crucifer crops in Nepal, and insecticides are used commonly against these. To evaluate alternative safe management practices, a field experiment was conducted with cabbage at Dailekh, Nepal, with eight treatments. The results revealed that azadirachtin 1500 ppm led to minimum leaf damage and higher yield. Spinosad 45% SC treated plot and control plot showed the maximum incidence.

ABSTRACT The cabbage aphid (Brevicoryne brassicae L.) is a cosmopolitan pest of brassica crops. The interactions among insect infestation, seed production, and seed physiology remain poorly understood. In this two-year field study, we evaluated silicon (Si) and ammonium sulfate (AS) as nutrient-based deterrents (applied at 25 and 50 kg ha− 1) compared to a conventional insecticide (Carbosulfan) for their effects on aphid resistance, seed yield, and seed nutrient physiology. Relative to the insecticide, Si at 25 kg ha− 1 reduced aphid populations by 16.2% and 13.5%, while Si at 50 kg ha− 1 reduced them by 24.3% and 21.9% in the first and second year, respectively. Similarly, AS at 25 kg suppressed aphids by up to 22.5% and 12.1% across the two years, whereas AS at 50 kg unexpectedly increased aphid numbers. Seed yield improved significantly with Si at 50 kg (28.7% and 30.9%), AS at 25 kg (14.6% and 15.9%), and AS at 50 kg ha− 1 (37.0% and 38.6%) in the first and second years, respectively. Nutrient analysis revealed that AS at 50 kg enhanced seed nitrogen, phosphorus, copper, and sulfur content, whereas Si at 50 kg increased potassium, magnesium, iron, calcium, zinc, and Si concentrations. These findings highlight the potential of Si and AS amendments in plant breeding programs to enhance pest resistance. Moreover, this study demonstrates the feasibility of optimizing fertilizer use for integrated pest management, reducing reliance on synthetic pesticides. Further research is needed to elucidate the underlying mechanisms by which these nutrients modulate plant defense against aphids.

Type-dependent biostimulant-mediated induction of nutrients and phenolic compounds improved plant tolerance against canola aphids (Brevicoryne brassicae L.).

Design, synthesis and Aphicidal activity of novel Pentafluorophenyl erivatives against Brevicoryne brassicae

Plants release signals to communicate with their environment. When damaged by insect herbivores, plants emit herbivore-induced plant volatiles (HIPVs) that can affect pest performance, recruit biocontrol agents, and trigger defence responses in neighboring plants. While plant communication via HIPVs has been well-documented over the past few decades, key aspects, such as the specific biochemical mechanisms underlying defence activation in neighboring plants and the variation in response to herbivory, remain poorly understood. This study aims to investigate the effect of HIPVs released by aphid (Brevicoryne brassicae)-infested Brassica napus (emitter) plants on neighboring uninfested conspecific plants (receiver). After 48 h exposure, emitter plants were discarded, and receiver plants were utilized in bioassays to assess aphid performance (fecundity and survival), preference, as well as amino acid and glucosinolates contents of receiver host plants. Results revealed that receiver plants showed a significant reduction in aphid fecundity, although no significant effect on aphid survival was observed. Aphid feeding preference was significantly altered, with B. brassicae showing a reduced preference for receiver plants. Additionally, significant increases in some key amino acids and total concentrations were recorded in receiver plants. Glucosinolates (GSs) analysis showed a significant change in total GS content, with a significant increase in the individual GSs, glucoiberin (GIB), and glucoraphanin (GRA). Current findings provide evidence that receiver plants activate their defence mechanism against B. brassicae by altering amino acid and glucosinolate levels. This study highlights the potential of HIPVs to enhance sustainable pest management strategies.

A field survey was conducted at vegetable ecosystems of Horticultural Experimental Farm, AAU, Jorhat to study the diversity of naturally occurring biological control agents during November 2022 to February 2023. Crops such as cabbage, radish, okra, pea, brinjal, chilli, mustard, spinach, brocolli, and roselle were grown in the field. Seven species of coccinellid predators (Cheilomenes sexmaculata, Propylea dissecta, Harmonia dimidiate, Coccinella transversalis, Micraspis univittata, C. septempunctata, H. octomaculata) were recorded from the above mentioned crops which are effective against several aphid species (Aphis gossypii Glover, A. craccivora Koch, Rhopalosiphum maidis (Fitch), Acyrthosiphon pisum (Harris), Brevicoryne brassicae Linnaeus, etc.). Among them, feeding potential of coccinellids species viz., C. sexmaculata and C. transversalis against mustard aphid (Lipaphis erysimi) was studied under laboratory conditions. Results indicated that the voracity of C. transversalis was higher (61.00 ± 1.32/adult/24hr) compared to C.sexmaculata (50.70 ± 1.04/adult/24hr) on L. erysimi.

In recent years, in the North-West region and the Leningrad region in particular, there has been a deterioration in the phytosanitary condition of spring rapeseed sowings, caused by an increase in the areas under this crop and an increase in the duration of its cultivation in farms. The aim of the research was to study the species diversity, abundance, structure of the aphid complex and population dynamics in spring rapeseed sowings at the agroecological station of the Menkovo branch of the Agrophysical Research Institute (Leningrad region). The research was carried out in 2021–2022 with the use of yellow water traps and route survey of the sowings. Aphids were counted in intervals of 7–8 days in July and the first twenty days of August. The aphid complex was characterized by high species diversity (15 species) and low abundance (2.0–2.1 specimens/trap). The numerical majority were species whose food specialization was associat-ed with rapeseed plants (43.5%), the remaining part was determined by the presence of weed vegetation (17.2%) and nearby agricultural crops (39.3%). Cabbage and mustard aphids were the dominant species, accounting for 23.5% and 19.8% of specimens, re-spectively. Mustard aphids were mainly caught in yellow traps in June, while cabbage aphids were caught in August. The maximum species diversity and abundance of aphids in spring rapeseed sowings were observed in the mid-July at the optimal sowing date (May 11, 2022) and in early August at the late sowing date (May 25, 2021). The higher number of cabbage aphid specimens was observed at the late sowing date of rapeseed compared to the optimal one.

Silicon-mediated Nutrient Deterrence Could Reduce Aphid (Brevicoryne brassicae L.) Abundance and Improve Crop Yield by Eliciting Phenolic Defense in Canola (Brassica napus L.)

This experiment was conducted in the fields of the College of Agriculture / University of Karbala on a cabbage crop during the growing season of 2024/2025. The aim was to evaluate the efficacy of certain integrated pest management components, specifically three botanical pesticides (Palizin, Tondexir, and Oxymatrine) and a bacterial biopesticide (Amyloland), as well as the synergistic effect of combining these components for the control of the cabbage aphid, Brevicoryne brassicae, under open field conditions. At the recommended concentration of 3 mL/L, Palizin demonstrated the highest mortality rate among the plant-derived insecticides, achieving a relative efficacy of 91.28% by day 21 post-application. In comparison, Tondexir and Oxymatrine yielded mortality rates of 72.48% and 73.3%, respectively. For Amyloland, the concentration of 3 g/L proved most effective, with a mortality rate of 67.2%, outperforming the 2 g/L and 2.5 g/L treatments, which resulted in 51.2% and 56.9% mortality, respectively. Regarding synergistic interactions, the combination of Palizin + Amyloland was superior, achieving complete aphid control (100% mortality) at 7, 14, and 21 days after application. This treatment outperformed both Tondexir + Amyloland and Oxymatrine + Amyloland, which recorded mortality rates of 76.7%, 94.7%, and 100%, and 80.3%, 99.4%, and 100%, respectively, over the same time intervals.

Rising concerns over chemical insecticides are demanding alternative pest management methods. The present study planned to explore the potential of elemental-ES, bio-sulphur-BS, compost-Cp, and compared to insecticide (Carbosulfan) and untreated controls for sustainable management of canola aphids (Brevicoryne brassicae). Split doses were more useful (screen house experiments) over single dose (in field). BS mixed with Cp provided best results to suppress aphid reproduction. Insecticide effects were instant but not long-lasting. Sulphur mixture also improved crop yield and oil contents; however, plant height was maximum in insecticide. RP-HPLC revealed activation of different phenolic defense chemicals (three flavonoids, five phenolic acids, and six hydroxycinnamic acid) in canola. Phenolic compounds were higher in sulphur-treated plants but their distribution patterns were altered in leaves, shoots, and seeds. The results could be extended to insect pests on other crops. However, further genetic studies are needed to discover the intricate pattern of sulphur resistance.

The study was conducted to evaluate the effectiveness of chemical and botanical insecticides against major cabbage insects at farmer field, District Dadu, Sindh during September-December 2023. Green Light” variety was sown in the first week of September, and the nursery was transplanted into the field during the first week of October. Seven treatments were applied including control viz., 10% Tobacco leaves extract, 10% Chinaberry fruit extract, 10% Neem leaves extract, Lambda cyhalothrin 2.5 EC, Imidacloprid 25% WP and Spinosad 240 SC, replicated 3 times. An untreated control plot was left blank as a check plot. Randomize Complete Block Design (RCBD) and Statistix 8.1 was used for analysis. Results show that all the tested treatments were found to be better than the control in reducing the cabbage larvae population. Spinosad 240 SC was found to be the most effective treatment with the lowest larvae population (0.89 and 0.77 larvae leaf-1) and maximum reduction of larvae population (73.04 and 84.01 %) after 1st and 2nd treatment application, respectively. Among botanical extracts, Neem leaf extract was the most effective, with the lowest larval population (1.18 and 1.04 larvae leaf-1) and the highest reduction (57.30% and 75.36%) after the 1st and 2nd treatment applications. The tested treatments were also found to be better than the control in reducing cabbage aphids’ population. Spinosad 240 SC was found the most effective treatment with the lowest aphids population (4.69 and 2.47 aphids leaf-1) and maximum reduction of aphid population (60.97 and 80.31 %) after 1st and 2nd treatment applications, respectively. Compared to other botanical extracts, Neem leaf extract showed the lowest aphid population (6.14 and 3.90 per leaf) with percent mortality of 48.74% and 68.00% after the 1st and 2nd applications. Resulting in the highest yield of cabbage (1005.33 kg ha-1) with cost-benefit ratio (of 5.45) was recorded in Spinosad 240SC, followed by imidacloprid 25%WP (992.33) with cost-benefit ratio (5.79). Among the botanical extracts, Neem leaf extract resulted in a yield of (685 kg/ha) with a cost-benefit ratio of (1.59) was recorded. It was concluded that all the tested insecticides were reducing the population of cabbage larvae and aphids. All the botanical extracts reduce the population of aphids and cabbage larvae, but as compared to other extracts neem extracts showed the best results among them. Hence using Imidacloprid 25% WP, Spinosad 240 SC at District Dadu is recommended for sustainable management of cabbage larvae and aphids.

The result revealed that the peak of population density of cabbage aphid Brevicoryne brassicae was 523.20 individuals/plant on 21 March in edges of rapeseed field and was 1141.67 individuals/plant in center of the field. Results revealed that population density of cabbage aphid in rapeseed fields surrounded by cover crops significantly were low compared with that of monoculture rapeseed. The location of rapeseed plants (in edges or in center) significantly affected (p

Cauliflower, a widely consumed vegetable valued for its nutrition in cooking, encounters significant agricultural difficulties because of the presence of various diseases that have an adverse impact on its quality and production. Early detection of these diseases is essential for timely plant treatment and increased production. This study presents a novel approach for detecting cauliflower leaf disease using deep learning techniques, taking use of the advances in deep learning for image classification. The study utilizes a dataset consisting of images of healthy leaves and affected leaves by widespread diseases such as Alternaria Leaf Spot, Black Rot, Cabbage Aphid, and Cabbage Looper. A pre-trained convolutional neural network (CNN) architecture is optimized and customized for this particular study in order to achieve disease classification. The proposed study has concentrated on fine-tuning the hyperparameters for the commonly used models such as NASNet Mobile, ResNet50, and Inception V3. The dataset used in this research contains 729 images of cauliflower leaves collected manually with the aid of a mobile phone camera from different farm fields in the Bhaktapur district of Nepal. Several performance metrics, such as accuracy, precision, and recall were used to evaluate the model’s performance. The experimental result shows that the ResNet50 has better performance with an accuracy of 93.47% compared to other models NASNet Mobile and Inception V3.

The cabbage aphid, Brevicoryne brassicae, is a major pest on Brassicaceae plants, causing significant yield losses annually. However, the lack of genomic resources has hindered progress in understanding this pest at the molecular level. Here, we present a high-quality, chromosomal-level genome assembly for B. brassicae, based on PacBio HiFi long-read sequencing and Hi-C data. The final assembled genome size was 429.99 Mb, with a scaffold N50 of 93.31 Mb. Notably, 96.19% of the assembled sequences were anchored to eight chromosomes. The genome covered 99.24% of BUSCO genes and 95.16% of CEGMA genes, indicating a high level of completeness. By integrating high-coverage transcriptome data, we annotated 22,671 protein-coding genes and 3,594 lncRNA genes. Preliminary comparative genomic analyses focused on genes related to host colonization, such as chemosensory- and detoxification-related genes, as well as cross-kingdom lncRNA Ya. In summary, this study presents a contiguous and complete genome for B. brassicae, which will advance our understanding of the molecular mechanisms underlying its host adaptation, pest behavior, and interaction with Brassicaceae plants.