Breeding Methods Research Articles

Innovative Cucumber Phenotyping: A Smartphone-Based and Data-Labeling-Free Model

Sustaining global food security amid a growing world population demands advanced breeding methods. Phenotyping, which observes and measures physical traits, is a vital component of agricultural research. However, its labor-intensive nature has long hindered progress. In response, we present an efficient phenotyping platform tailored specifically for cucumbers, harnessing smartphone cameras for both cost-effectiveness and accessibility. We employ state-of-the-art computer vision models for zero-shot cucumber phenotyping and introduce a B-spline curve as a medial axis to enhance measurement accuracy. Our proposed method excels in predicting sample lengths, achieving an impressive mean absolute percentage error (MAPE) of 2.20%, without the need for extensive data labeling or model training.

Features of the reproductive biology of representatives of the genus Haslea Simonsen (Bacillariophyta), allowing the use of classical breeding methods

The application of the classical selection of diatoms on the example of the genus Haslea is considered. The ability of some representatives of this genus to synthesize marennine and marennine-like pigments is interesting to researchers and owners of oyster farms because these unique pigments can colorise the tissues of mollusks in a greenish color and improve their organoleptic properties. It has been shown that marennine has antibacterial, antiviral, antioxidant and other biological activities. It is assumed that the efficiency of marennine biosynthesis can be increased by obtaining highly productive strains by selection, which was not previously carried out in diatoms. The assessment of the possibility and prospects of applying the methods of classical breeding in relation to representatives of the genus Haslea is given. It is shown that significant success has been achieved to date in the study of the life cycle, crossing systems and other features of the reproductive biology of representatives of the genus Haslea, which allows them to carry out classical selection by selection, hybridization and obtaining descendants of the next generation.

Assessment on dairy goat management practice in the Korahey Zone, Somali, Ethiopia

ABSTRACT The characterization of dairy goat management practices in the Korahay Zone, Somali Regional State, was the aim of this study. The woredas and respondents were selected purposefully. Parameters, such as feeding management, housing, watering, healthcare, breeding and selection methods, were collected. A semi-structured questionnaire was used to collect data. The collected data were analysed using the statistical package for social science (SPSS) version 20. According to the result, natural pasture (22.5%, 45% and 42.5%) is the main feed source and free-ranging (92.5%, 100% and 100%) is the commonly practised feeding system (p 0.05) for goats in Shegosh, Kabridahar and Shilabo, respectively. Ponds and boreholes were the main water sources for goats. The majority of respondents in study areas practised uncontrolled breeding systems (p 0.05), due to goats’ grazing together, insufficient breeding buck and lack of awareness (p 0.05). The confirmation of the buck was ranked as the first important criterion for the selection of breeding bucks in selected districts. However, the size and appearance of the doe in the Shegosh and Kebridahar districts and better milk yield in the Shilabo district were the first-ranked criteria for the selection of breeding does. Therefore, it is recommended that government attention be provided to pastoralists in goat management.

Streamlining Different Plant Breeding Methods to Enhance Water Use Efficiency in Agricultural Systems

The relevance of Water in human life and agro-ecosystems has been extensively illustrated for decades around the globe. Despite the reality that the earth is mostly surrounded by water, its utility has been limited to domestic consumption and agricultural purposes. Thus, tweaking water usage in agricultural systems has been a tricky problem in order to meet the requirements of the growing population and to obtain more crop yield per drop of water in agriculture. In this review, an attempt has been made to revisit the methods available to improve water use efficiency in agriculture.

Development of Game Integrated Learning Module for Animal Breeding Subtopic for Year Two Science

Game is one of the elements that can be applied in teaching and learning sessions to enhance students’ motivation in learning the topics being taught. Therefore, this study was conducted to develop a game-integrated learning module for the subtopic of Animal Breeding for Science Year Two. The module was developed based on the five phases in the ADDIE instructional design model. However, this study only focuses on the development and validity analysis of the module. The module applies a cross-curricular element as students are asked to construct masks of animals which lay eggs and give birth. The module also integrates the game element that requires students to wear the constructed animal masks and gather around according to the selected animal categories. The developed learning module was evaluated from face and content validity aspects by three experts. The validity analysis indicated that the module has high expert agreement percentages in terms of face validity (93%) and content validity (92%). The developed game-integrated learning module is expected to attract students' interest in learning Science and increase their understanding on the animal breeding method.

Determination of Lethal Dose 50 for Induced Mutagenesis in Soybean [Glycine max (L.) Merril] cv. Gepak Kuning Through Ethyl Methane Sulfonate Mutagen

Background: The improvement of the genetic and agronomic properties of cultivated plants can be carried out by various plant breeding methods, including mutation breeding. Mutations in nature are very few and rare. Ethyl Methane Sulfonate (EMS) is a chemical mutagen that can cause random mutations resulting in changes in genetic material. This study aims to determine the effect of EMS on the germination of soybean (cv. Gepak Kuning) seeds. Methods: The research was conducted at the Faculty of Agriculture Laboratory, Universitas Malikussaleh, Indonesia, in January 2023. The concentrations of EMS used were 0%, 0.025%, 0.05%, 0.1%, 0.25%, 0.5% and 1% with a soaking time of 4 hours. EMS concentration of 0% was used as a control treatment. The observed variables were made as percentage of germination (%) and seedling height at 7th day and 14th day after planting. Result: The results showed that an increase in EMS concentration affected the percentage of germination of soybean (cv. Gepak Kuning) sprouts. A decrease in the percentage of germination was found at an EMS concentration of 0.5%, while a decrease in seedling height had begun to be seen at an EMS concentration of 0.25%. The results of the analysis with CurveExpert 1.4 software showed that the LD50 value of the seeds of soybean (cv. Gepak Kuning) treated with EMS mutagen was at a concentration of 0.63%, the LD20 dan LD30 value was at a concentration of 0.25% and 0.41%, respectively.

From Petri Dish to Field: Plant Tissue Culture and Genetic Engineering of Oats for Improved Agricultural Outcomes

Oats (Avena sativa) hold immense economic and nutritional value as a versatile crop. They have long been recognized as an exceptional choice for human consumption and animal feed. Oats’ unique components, including proteins, starches, and β-glucans, have led to its widespread use in various food products such as bread, noodles, flakes, and milk. The popularity of oat milk as a vegan alternative to dairy milk has soared due to the increasing number of vegetarians/vegans and growing environmental awareness. Oat milk offers a sustainable option with reduced greenhouse gas emissions during its production, rendering it an appropriate choice for individuals who are lactose-intolerant or have dairy allergies. To ensure improved adaptability and enhanced nutrition, the development of new oat varieties is crucial, considering factors like cultivation, climate, and growing conditions. Plant cell culture plays a crucial role in both traditional and contemporary breeding methods. In classical breeding, plant cell culture facilitates the rapid production of double haploid plants, which can be employed to accelerate the breeding process. In modern breeding methods, it enables genetic manipulation and precise genome editing at the cellular level. This review delves into the importance of oats and their diverse applications, highlighting the advantages of plant cell culture in both classical and modern breeding methods. Specifically, it provides an overview of plant tissue culture, encompassing genetic transformation, haploid technology, protoplast technology, and genome editing.

Drought stress tolerance in rice: a critical insight

Drought is currently a serious threat for farming especially in rice cultivation, due to its substantial water requirements throughout its lifecycle. Drought is one of the major environmental constraints disrupting the growth and yield of rice plants, affecting them at physiological, morphological, biochemical and molecular levels. Global climate change exacerbates this issue, leading to substantial economic losses. As rice is a major food crop worldwide, the demand for rice production is increasing in tandem with the expanding human population. Consequently, it has become imperative to utilize drought-prone areas for agriculture and develop drought-tolerant rice genotypes. In addition to conventional breeding methods, the application of multi-omics approaches proves most effective in meeting the need to enhance drought tolerance in rice plants. Protective mechanisms, such as morphological adaptation, physiological acclimatization, cellular adjustments and antioxidant defense, play pivotal roles in helping plants overcome drought stress. Plant-microbial interactions are important for plants to overcome drought-induced adversities. Furthermore, applications of conventional approaches, omics approaches and nanotechnology are very promising for generating climate smart agriculture. Our aim in this review is to focus on drought stress tolerance in rice including drought-tolerant rice genotypes, their adaptation mechanisms, the unveiling the genes, transcription factors, microRNAs (miRNA) involved, microbial assistance and exploring approaches to mitigate drought stress in rice plants. The present review might throw some light on understanding the mechanism of drought stress tolerance in rice, including its molecular crosstalk and biochemical dynamics, for future researchers.

Speed Breeding for Crop Improvement and Food Security

Amid a rapidly growing global population and increasing threats to crop yields, this review focuses on Speed Breeding (SB) in crop genetics. It traces SB’s development from carbon arc lamp experiments 150 years ago to its modern use with LED technology which significantly accelerates breeding cycles. SB has applications in genetic mapping, genetic modification, and trait stacking, enhancing crop resilience by leveraging allelic diversity. It aligns well with breeding methods like single plant selection and single seed descent. The integration of SB with gene editing, genotyping, and genomic selection holds great promise. However, SB faces challenges related to infrastructure, genotypic variations, and potential stress responses. In summary, SB is a powerful and promising approach to address food security concerns and advancing crop genetics.

Replenishment of the species composition of the bioresource collection of Entomoacariphages

Aim. Formation of the species composition of the bioresource collection "State Collection of Entomoacariphages and Microorganisms" of the Lazarev Experimental Plant Protection Station – a branch of Federal Scientific Centre for Biological Plant Protection. The collection is based on species identified in local biocenoses as well as those introduced from regions with similar climatic conditions.Material and Methods. Collection and study of the effectiveness of native, invasive, introduced entomophages, the dynamics of their acclimatization and the possibility of their introduction into laboratory culture on the territory of the Black Sea coast of Sochi. Methods of their application in plant protection systems were tested.Results. The ability of Cryptolaemus montrouzieri Muls. to control the number of coccids, including Icerya purchase Maskell, has been experimentally established. Entomophages which are effective and adaptable to reproduction in laboratory conditions were selected: Dicyphus errans Wolff. and Macrolophus nubilis H.S., the aphidophage Harmonia axyridis Pall. and others.Conclusion. As a result of the study, wide polyphages of Dicyphus errans Wolff were found to be presented in the collection of the Lazarev Experimental Plant Protection Station, as well as and Macrolophus nubilis H.S., Cryptolaemus montrouzieri Muls., Leis dimidiata Fabr., Harmonia axyridis Pall., Encarsia partenopea Masi and Lysiphlebus fabarum Marsh. Acariphages are represented by Phytoseiulus persimilis Ath.‐Henr. and Amblyseius cucumeris Ond. The biological efficacy of Encarsia partenopea Masi has been evaluated in relation to Trialeurodes vaporariorum Westw. in the conditions of the protected ground of Sochi at the parasite ratio:host of 1:30; 68.6%, the best result being obtained with releases of 1:10; 79.19%. Research has begun on the development of methods for the laboratory breeding of Chilocorus renipustulatus.

Generation Mean Analysis for quantitative traits in the population of AKDRMS 21-54 x YH3 cross of rice (Oryza sativa L.)

Aim: To study the genetic components of inheritance for 12 quantitative traits which leads into genetic improvement by identification of gene actions for yield and yield related traits. Methodology: Twelve quantitative traits for yield and yield attributing traits were studied at Agricultural College, Bapatla during Rabi 2021-22 deploying Hayman's five parameter model generation mean analysis in estimating the gene effects in the population (P1, P2, F1, F2 and F3) generated from the cross AKDRMS 21-54 x YH3. Estimated mean effects affirm the existence of significant variation in the traits studied. Significance of either one or both, scales C or D inferred the existence of epistatic interaction for the traits studied. Digenic non-allelic interaction model explained the gene action of the traits studied in the present investigation. Results: The generation mean for all the traits showed the importance of both additive and dominance type of gene effects. Among the epistatic gene effects, the additive x additive gene interaction was reported predominant for shoot length, plant height, flag leaf length, spikelet fertility and flag test weight. The gene interaction is associated with homozygosity, therefore, pedigree method of breeding may be adopted for isolation of desirable lines, while for all other traits, along with grain yield per plant, dominance x dominance gene interaction was found to be pre-dominant. The gene interaction is not fixable, hence, population improvement approaches, would be effective. Interpretation: Dominance x dominance gene interaction was predominant for grain yield per plant and majority of yield component traits studied coupled with duplicate epistasis, infers to takeup population improvement approaches, such as bi-parental mating and recurrent selection, followed by isolation of purelines in later generations for competent improvement. Key words: Epistasis, Five parameter model, Generation Mean Analysis, Quantitative traits, Rice

Development, results and prospects of the spring durum wheat breeding in Russia (post-Soviet states).

The article outlines a brief historical background on the introduction to cultivation, distribution and breeding of spring durum wheat in the steppe and forest-steppe regions of Eurasia (the countries of the former USSR: Russia, Ukraine, and Kazakhstan). The approaches and methodology for improving durum wheat during certain scientific selection periods are given. The features of the selection program implementation and the breeding scale expansion during the creation of breeding stations at the beginning of the XX century, after the end of the Great Patriotic War, in the second half of the XX century, and at present are considered. A characteristic according to the main features and properties of varieties created in different periods is given. The achievements of the classical breeding method by comparing old and new varieties are analyzed. The efficiency and rate of wheat selection by periods in different regions of Russia is estimated. The results and methods of breeding for yield, resistance to drought, leaf diseases (Stagonospora nodorum Berk., Septoria tritici (Roeb. et Desm.), Bipolaris sorokiniana (Sacc.) Shoemaker, Pyrenophora tritici repentis (Died.) Drechs., Fusarium sp., Puccinia titicina Eriks., Puccinia graminis Pers. f. sp. tritici Eriks., Blumeria graminis (DC.) f. sp. tritici Em. Marchal), grain pathogens Ustilago tritici (Pers.) Rostr.) and pathogens causing darkening of the corcule and endosperm (Bipolaris sorokiniana (Sacc.) Shoemaker, Alternaria tenuis (Nees et Fr.), Аlternaria triticina (Prasada & Prabhu)), pests (Cephus pygmeus Lens, Osinosoma frit L., Mayetiola destructor (Say)), grain quality (protein content, amount of yellow pigments, dough rheology, sprouting resistance) and end products are presented. The prospects for the molecular marker application for a number of traits in breeding in the near future are given.

QTL mapping in sesame (Sesamum indicum L.): A review.

Sesame (Sesamum indicum L.) is an important oilseed crop used for food, feed, medicinal, and industrial applications. Inherently low genetic yield potential and susceptibility to biotic and abiotic stresses contribute to low productivity in sesame. Development of stress resistant varieties coupled with high yield is a viable option to raise the genetic potential of sesame. Conventional phenotype-based breeding methods have made an important role in the last couple of decades by developing several sesame varieties with improved quality, yield, and tolerance to biotic and abiotic stresses. However, due to adverse environmental effects, time consuming to develop new variety, and low genetic gain, conventional phenotype-based approach is not adequate to satisfy the rising population growth. In this context, advanced method of genotype selection via modern techniques of biotechnology plays essential roles in reducing the constraints and boosting sesame production to satisfy the huge demand. In line to this, quantitative trait loci (QTL) mapping is considered as a promising method to address the problems of sesame breeding. Previously, huge data have been generated in the practical use of QTL for sesame improvement. Therefore, this paper aims to review recent advances in the area of QTL mapping for yield and yield related traits in sesame for enhancing and sustaining sesame production. In this section, we present an intensive review on the identification and mapping of the most desirable potential candidate genes/QTLs associated with desirable traits. Moreover, this review focuses on the major QTL regions and/or potential candidate genes and associated molecular markers that could provide potential genetic resources for molecular marker-assisted selection and further cloning of functional genes for yield and yield-related traits as well as various biotic and abiotic stress tolerances. Finally, the summarized QTL mapping data shed light on future directions for enhanced sesame breeding programs.

Challenges for crop improvement.

The genetic improvement of crops faces the significant challenge of feeding an ever-increasing population amidst a changing climate, and when governments are adopting a 'more with less' approach to reduce input use. Plant breeding has the potential to contribute to the United Nations Agenda 2030 by addressing various sustainable development goals (SDGs), with its most profound impact expected on SDG2 Zero Hunger. To expedite the time-consuming crossbreeding process, a genomic-led approach for predicting breeding values, targeted mutagenesis through gene editing, high-throughput phenomics for trait evaluation, enviromics for including characterization of the testing environments, machine learning for effective management of large datasets, and speed breeding techniques promoting early flowering and seed production are being incorporated into the plant breeding toolbox. These advancements are poised to enhance genetic gains through selection in the cultigen pools of various crops. Consequently, these knowledge-based breeding methods are pursued for trait introgression, population improvement, and cultivar development. This article uses the potato crop as an example to showcase the progress being made in both genomic-led approaches and gene editing for accelerating the delivery of genetic gains through the utilization of genetically enhanced elite germplasm. It also further underscores that access to technological advances in plant breeding may be influenced by regulations and intellectual property rights.

Effect of X-rays on Seedling Pigment, Biochemical Profile, and Molecular Variability in Astrophytum spp.

Cacti are important in agricultural economies and one of the most popular horticultural plant groups. The genus Astrophytum is one of the most valuable and desirable cacti for growers and collectors around the world. By selecting the appropriate breeding methods to induce variations in combination with modern biotechnology tools for rapid change detection, it is possible to meet the challenges of the modern world in creating new variability in plants. However, there exists a lack of research concerning the impact of ionizing radiation on cacti. The aim of the study was to assess the effects of X-rays at different doses (0 Gy—control, 15, 20, 25, and 50 Gy) on the dynamics of seed germination in vitro, changes in the color of seedlings, biochemical changes in the content of metabolites and changes at the molecular level in Astrophytum spp. ‘Purple’. A significant effect of X-rays on the induction of genetic variation was observed. Remarkably high polymorphism rates were observed, ranging from 59.09% for primer S12 to a full 100.0% for S3 and S8, as determined by the SCoT (Start-Codon-Targeted) marker. In addition, a large variation in the content of plant pigments (anthocyanins, carotenoids, chlorophyll a, and chlorophyll b) was noted. Additionally, discernible alterations in the color of the tested cactus seedlings, assessed by the RHSCC catalog, were attributed to the impact of ionizing radiation. These findings hold promise for the application of radiomutation breeding in acquiring new cactus cultivars.

A critical review on integrating bio fortification in crops for sustainable agricultural development and nutritional security

Numerous developing nations face epidemics that are characterized by nutritional deficiencies in both humans and animals. A further threat to nutritional quality is posed by the lack of dietary diversity, which consists predominantly of cereal-based crops lacking in essential mineral nutrients. Cereals and pulses are major food crops in backward countries; however, they often lack essential compounds, macronutrients, and micronutrients, resulting in imbalanced nutrition. Widespread malnutrition, characterized by ailments such as anemia, rickets, and scurvy, is a direct consequence of this nutritional imbalance. It is crucial to bio-fortify cereals and pulses to provide the population with balanced diets and reduce malnutrition. New breeding techniques (NBTs) such as gene editing, gene overexpression, and gene transfer from wild relatives offer alternative avenues to obtain crops with optimal nutritional profiles. This review delves into the significance of bio fortification in enhancing food crops and explores the utilization of advanced breeding methods for the development of novel bio fortified crop varieties, thereby tackling nutritional security within the realm of agriculture. It conveys a message to researchers regarding the considerable potential of bio fortification to enhance crop productivity while enriching crops with additional nutrients.

Investigating the response mechanisms of bread wheat mutants to salt stress

Mutation breeding is among the most critical approaches to promoting genetic diversity when genetic diversity is narrowed for a long time using traditional breeding methods. In the current study, 15 wheat mutants created by gamma radiation and three salt-tolerant wheat cultivars were studied under no salinity stress (Karaj) and salinity stress (Yazd) during three consecutive growing seasons from 2017 to 2020 (M05 to M07 generations mutants). Results showed that salinity induced lipid peroxidation and enhanced ion leakage in all genotypes however, M6 and M15 showed the least ion leakage increment. It was also observed that the activity of antioxidant enzymes including SOD, CAT, POX, APX and GR increased with salinity; the maximum increase in antioxidant activity was belonged to M15, M09, M06 and M05. All genotypes had higher protein content in salinity stress conditions; M07 and M12 showed the lowest (1.8%) and the highest (17.3%) protein increase, respectively. Zeleny sedimentation volume increased under salinity stress conditions in all genotypes except M06, C2, C3, and M07. The result indicated that salinity stress increased wet gluten in all genotypes. M10 and M08 showed the highest (47.8%) and the lowest (4%) wet gluten increment, respectively. M06 and M11 mutants showed the lowest (6.1%) and the highest (60.7%) decrement of grain yield due to salinity stress, respectively. Finally, M04, M05, M07, M13, and M14 were known as genotypes with high grain yield in both no salinity and salinity stress conditions. In other word, these genotypes have higher yield stability. The results of the current study revealed that gamma irradiation could effectively be used to induce salinity tolerance in wheat.

Estimation of Genetic Parameters and Identification of Leaf Blast-Resistant Rice RILs Using Cluster Analysis and MGIDI

Rice blast disease, caused by the fungus Magnaporthe oryzae, poses a significant threat to rice cultivation. One effective way to deal with this disease is to identify and introduce resistant varieties using different breeding methods. This study utilized a population of 153 recombinant inbred lines (RILs) derived from the crossing of the Shahpasand (SH) and IR28 varieties, characterized by susceptibility and resistance to leaf blast, respectively. In combination with 12 control varieties, these genotypes were subjected to an extensive evaluation of disease severity (5 stages), the area under the disease progress curve (AUDPC), type, and the infection rate in 2021 and 2022. Analysis of variance revealed significant genetic variation, highlighting the potential of the RIL population for identifying and selecting resistant lines. Employing cluster analysis and the multi-trait genotype-ideotype distance index (MGIDI), 17 lines were identified as the most resistant over a two-year evaluation period. The average AUDPC for these resistant lines was estimated at 2.435 ± 0.114, and lines 17 and 111 had the lowest AUDPC (1.526 and 1.630, respectively) and showed the least infection in two years. Conversely, lines 42 and 43 showed the highest AUDPC values (255.312 and 248.209) along with heightened sensitivity. The use of MGIDI yielded a substantial selection differential (SD) of −59.12% for traits related to leaf blast disease resistance, demonstrating the effectiveness of this method. Furthermore, new recombinant populations are expected to be developed in future plant breeding projects by crossing the most susceptible and resistant lines, which will be new sources of resistance to this disease.

IMPROVING THE QUALITY OF VEGETABLES AND FRUITS USING BIOFORTIFICATION STRATEGIES

An analysis of the state of nutrition of people in Ukraine shows that the population does not eat well, which significantly worsens the state of their health and working capacity. The solution to the problem is possible through changes in the structure of nutrition, and above all, through an increase in the diet of vegetables and fruits of high quality, the varieties of which have been created using biofortification strategies. Biofortification is an innovative and promising approach in solving global problems related to nutrition. The goal of biofortification is to create plants with increased levels of the necessary functional ingredients absorbed from the soil, as well as those synthesized in the plant. Biofortification strategies consist in the development of optimal ways of mineral nutrition of plants, the creation of new species using molecular genetic approaches, and the use of traditional breeding methods. The purpose of the research is to analyze the modern experience of creating varieties of various fruit and vegetable crops with the help of biofortification strategies, which have an increased content of functional ingredients. Based on the literature review, it was established that for many years, an unconventional direction of selection has been developing – the creation of varieties and hybrids of fruits and vegetables with the recommended chemical composition. Created varieties of vegetables and fruits with increased protein content (nuts and legumes); starch (potato); carotenoids, dry matter, disaccharides (carrot, pumpkin); vitamin C, betanin, sugars (table beet); selenium (tomatoes); bioflavonoids, vitamin C (cabbage vegetables); macro- and microelements (bulb vegetables), etc. Such raw materials can be used to create special purpose products, which will significantly improve the quality of the population's diet and health. It is shown that the development of the non-traditional direction in breeding remains promising, and the study of samples of the domestic collection of agricultural crops and the selection of the best varieties from them for various directions of use, and especially for the production of special purpose products, is an important national economic and scientific task of an innovative nature that must be developed, therefore that the problem of improving the nation should come first.

Genome editing for healthy crops: traits, tools and impacts.

Crop cultivars in commercial use have often been selected because they show high levels of resistance to pathogens. However, widespread cultivation of these crops for many years in the environments favorable to a pathogen requires durable forms of resistance to maintain "healthy crops". Breeding of new varieties tolerant/resistant to biotic stresses by incorporating genetic components related to durable resistance, developing new breeding methods and new active molecules, and improving the Integrated Pest Management strategies have been of great value, but their effectiveness is being challenged by the newly emerging diseases and the rapid change of pathogens due to climatic changes. Genome editing has provided new tools and methods to characterize defense-related genes in crops and improve crop resilience to disease pathogens providing improved food security and future sustainable agricultural systems. In this review, we discuss the principal traits, tools and impacts of utilizing genome editing techniques for achieving of durable resilience and a "healthy plants" concept.