Brazilian Group Research Articles

Comparison between flow cytometry and standard PCR in the evaluation of MRD in children with acute lymphoblastic leukemia treated with the GBTLI LLA – 2009 protocol

Minimal residual disease (MRD) monitoring is of prognostic importance in childhood acute lymphoblastic leukemia (ALL). The detection of immunoglobulin and T-cell receptor gene rearrangements by real-time quantitative PCR (RT-PCR) is considered the gold standard for this evaluation. However, more accessible methods also show satisfactory performance. This study aimed to compare MRD analysis by four-color flow cytometry (FC) and qualitative standard PCR on days 35 and 78 of chemotherapy and to correlate these data with patients’ clinical characteristics. Forty-two children with a recent diagnosis of ALL, admitted to a public hospital in Brazil for treatment in accordance with the Brazilian Childhood Cooperative Group for ALL Treatment (GBTLI LLA-2009), were included. Bone marrow samples collected at diagnosis and on days 35 and 78 of treatment were analyzed for the immunophenotypic characterization of blasts by FC and for the detection of clonal rearrangements by standard PCR. Paired analyses were performed in 61/68 (89.7%) follow-up samples, with a general agreement of 88.5%. Disagreements were resolved by RT-PCR, which evidenced one false-negative and four false-positive results in FC, as well as two false-negative results in PCR. Among the prognostic factors, a significant association was found only between T-cell lineage and MRD by standard PCR. These results show that FC and standard PCR produce similar results in MRD detection of childhood ALL and that both methodologies may be useful in the monitoring of disease treatment, especially in regions with limited financial resources.

Oral health complications in Brazilian and French diabetic older people: A comparative study

IntroductionDiabetes mellitus (DM) is a risk factor for periodontitis for over 40 years and novel evidence suggests that periodontitis has an impact on glycemic control in patients with diabetes. This study aimed to compare oral health complications in diabetic older patients from Brazil and France. MethodsThis cross-sectional study included 120 patients aged 65 and over diagnosed with type 2 diabetes. Sixty patients were admitted to a center for diabetes and hypertension care in Brazil and 60 patients were admitted to the Rouen University hospital. Dental conditions were assessed through the decayed, missing and filled teeth index and periodontal condition was assessed using the Community Periodontal Index. The significance threshold was p < 0.05. ResultsDecayed teeth differed statistically between the groups (p = 0.001). The French group presented more tooth mobility, gingival recession and furcation involvement (p < 0.001). Tooth brushing frequency differed significantly between the groups (p < 0.030). The main cause of missing teeth was periodontitis in the French group and caries and periodontitis in the Brazilian group (p < 0.001). Statistical significance was found for use of fixed upper (p = 0.013) and lower (p = 0.013) dentures in the French group. The French group needed upper denture rehabilitation (p = 0.010) while the Brazilian group needed lower denture rehabilitation (p = 0.003). ConclusionEdentulism was prevalent in diabetic older people in both countries. However, the French participants presented with better oral health.

Anthropometric and metabolic profile of a Brazilian Amerindian group: The Xikrin (Mebengôkre).

The purpose of this study was to describe the metabolic and anthropometric profile of the Xikrin (Mebengôkre), an indigenous group. A total of 363 subjects (55.1% women) aged 18 years or older were evaluated. The variables analyzed were age, body weight and height, waist circumference, blood pressure, fasting blood glucose, triglyceride level, and total cholesterol level. A high prevalence of obesity (36.5%) and central obesity (88.1%), mainly among women (46.9% and 96.2%, respectively), was found among the Xikrin (Mebengôkre). Impaired fasting glycemia and diabetes were found in 4.5% and 3.8% of adults, respectively. Twenty-one percent of adults had dyslipidemia and 9.3% had hypertension. The high prevalence of excess weight (overweight and obesity) and central obesity, especially in women, was the most significant finding of this study among the Xikrin (Mebengôkre).

Development and validation of a prediction model for long-term unsuccess of early treatment of Class III malocclusion.

To develop and validate a prediction model to forecast long-term stability of early treatment with rapid maxillary expansion (RME) and facemask (FM) in a large sample of Class III growing patients. The Brazilian Group (BG) consisted of 73 consecutively treated Caucasian Class III patients (41 females and 32 males). Mean age at T0 (before treatment) was 7.1 ± 1.6 years, while mean age at T1 (long-term follow-up) was 21.8 ± 3.2 years. The Italian Group (IG, validation cohort) comprised 28 consecutively treated Caucasian Class III patients (14 females and 14 males, mean age at T0 9.0 ± 1.3 years and mean age at T1 18.2 ± 1.4 years). Cephalometric analysis was performed on lateral cephalograms at T0. Gender and cephalometric variables, chronologic age, and dentition phase at T0 were used as predictors for long-term unsuccessful treatment at T1. All predictors for unsuccessful treatment in the BG were subjected to bivariate logistic regression. Only those statistically significant predictors in the bivariate logistic regression entered mixed stepwise logistic regression with P = 0.05 to enter and to leave. The validity of the prediction model derived from the BG was then tested on the IG. The prediction model consisted of only one cephalometric variable: the angle between the Condylar Axis and the Mandibular Plane (CondAx-MP) (odds ratio: 1.52, 95% confidence interval: 1.25-1.85, P < 0.0001). Unsuccessful treatment at T1 was predicted for values of CondAx-MP at T0 greater than the cut-off value of 147.8 degrees. BG patients predicted incorrectly were 3 out of 22 for the unsuccessful cases and 1 out of 51 for the successful cases. Therefore, accuracy was 0.95, sensitivity 0.86, specificity 0.98, and positive and negative predictive values were 0.95 and 0.94. When the predictive model was applied on IG, all five unsuccessful cases were predicted correctly, while only 1 out of 23 successful patients was predicted incorrectly. CondAx-ML was identified as a reliable predictor for long-term stability of early Class III treatment with RME and FM.

Brazilian recommendations on the safety and effectiveness of the yellow fever vaccination in patients with chronic immune-mediated inflammatory diseases

BackgroundIn Brazil, we are facing an alarming epidemic scenario of Yellow fever (YF), which is reaching the most populous areas of the country in unvaccinated people. Vaccination is the only effective tool to prevent YF. In special situations, such as patients with chronic immune-mediated inflammatory diseases (CIMID), undergoing immunosuppressive therapy, as a higher risk of severe adverse events may occur, assessment of the risk-benefit ratio of the yellow fever vaccine (YFV) should be performed on an individual level.Main body of the abstractFaced with the scarcity of specific orientation on YFV for this special group of patients, the Brazilian Rheumatology Society (BRS) endorsed a project aiming the development of individualized YFV recommendations for patients with CIMID, guided by questions addressed by both medical professionals and patients, followed an internationally validated methodology (GIN-McMaster Guideline Development). Firstly, a systematic review was carried out and an expert panel formed to take part of the decision process, comprising BRS clinical practitioners, as well as individuals from the Brazilian Dermatology Society (BDS), Brazilian Inflammatory Bowel Diseases Study Group (GEDIIB), and specialists on infectious diseases and vaccination (from Tropical Medicine, Infectious Diseases and Immunizations National Societies); in addition, two representatives of patient groups were included as members of the panel. When the quality of the evidence was low or there was a lack of evidence to determine the recommendations, the decisions were based on the expert opinion panel and a Delphi approach was performed. A recommendation was accepted upon achieving ≥80% agreement among the panel, including the patient representatives. As a result, eight recommendations were developed regarding the safety of YFV in patients with CIMID, considering the immunosuppression degree conferred by the treatment used. It was not possible to establish recommendations on the effectiveness of YFV in these patients as there is no consistent evidence to support these recommendations.ConclusionThis paper approaches a real need, assessed by clinicians and patient care groups, to address specific questions on the management of YFV in patients with CIMID living or traveling to YF endemic areas, involving specialists from many areas together with patients, and might have global applicability, contributing to and supporting vaccination practices. We recommended a shared decision-making approach on taking or not the YFV.

Cenários de pesquisa com e sobre crianças, mídia, imagens e corporeidade

Ao pensar a relação entre mídia, imagens e corporeidades na pesquisa com e sobre crianças, o texto discute alguns cenários e práticas culturais das infâncias contemporâneas a partir do olhar da educação. Apresenta-se os fundamentos teórico-metodológicos que têm orientado investigações desenvolvidas num grupo de pesquisa brasileiro nos últimos quatro anos, em sintonia com abordagens presentes em outros contextos nacionais e internacionais. Numa compreensão de pesquisa como espaço de reflexão crítica e instância pedagógica, as investigações escolhidas para compor o recorte do artigo entrelaçam os temas mídia, imagem e corporeidade com a atenção aos direitos das crianças, à ludicidade, às autorias infantis e aos desafios das práticas culturais na cultura digital. Entre tais desafios, destaca-se a necessidade de considerar a escuta às vozes, aos silêncios e às performances das crianças como forma privilegiada para construir metodologias participativas. Sinaliza-se também a importância de desconstruir certas retóricas redutoras presentes no debate social e dar visibilidade à riqueza do já considerável corpo de pesquisas sobre imagem, mídia e corporeidade na infância. Por fim, o texto enfatiza o papel da articulação interdisciplinar entre pesquisadores que atuam com estes temas, de modo a fortalecer a pesquisa voltada a qualificar a mediação educativa diante das mudanças culturais contemporâneas.

Características da colaboração científica entre grupos de pesquisa de áreas de exatas, vida e humanas

Resumo Este estudo caracteriza a colaboração científica (CC) entre grupos de pesquisa (GP), atuantes nas áreas de exatas, vidas e humanas, abordando motivos da colaboração; dinâmica de interação; facilitadores, desafios, problemas e dificuldades enfrentados; principais resultados alcançados; e conteúdo aprendido. A pesquisa, de natureza teórico-empírica, descritiva e qualitativa, pautou-se em revisão bibliográfica para situar o panorama de estudos acerca dessa temática. Os dados coletados com base em roteiro de entrevista semiestruturado foram organizados em categorias definidas a priori e submetidos a análise de conteúdo. Os resultados indicam a necessidade de parcerias para atender a editais de fomento ou visando principalmente: 1) à ampliação de estudos e pesquisas, com diversificação de perspectivas teóricas e metodológicas, com o objetivo de obter maior conhecimento a respeito do tema, além do aproveitamento e troca de conhecimentos e experiência de outros integrantes da CC; e 2) ao compartilhamento de recursos financeiros e equipamentos. Contribuem para a formação de parcerias a proximidade entre as linhas de pesquisa e o conhecimento prévio dos pesquisadores, decorrente de situações de formação ou interação em eventos. Há sobreposição entre desafios, problemas, aspectos dificultadores e facilitadores, sendo a presença ou ausência de algumas condições determinante de como aquele aspecto é percebido. Entre os principais resultados estão o melhor aproveitamento de recursos, a melhoria da qualidade e a ampliação da produção e da disseminação do conhecimento. Além de focar o tema em contexto de GP brasileiros de diferentes áreas do conhecimento, o estudo contribui com a identificação de conteúdos aprendidos na atuação em CC.

WEEKLY PROFILE OF TRAINING LOAD AND RECOVERY IN ELITE RHYTHMIC GYMNASTS

The aim of this study is to analyze the weekly profile of internal training load (ITL) and recovery of elite rhythmic gymnasts during a season. Eight professional rhythmic gymnasts of the Brazilian senior group participated. The session rating of perceived exertion (session-RPE) and Total Quality Recovery (TQR) score were collected daily across a 37-week season. The session-RPE was collected after each session and the TQR before the first session of the day. The sum of ITL of each session of the day (dITL) and week (wITL), as well as average TQR scores, were retained for the analysis. Training monotony and strain were also recorded. For the analysis, the season was divided into preparatory period, competitive period and a period comprising the competition weeks, within the competitive period. The ITL and recovery profile were different between the days of the periods and the competition weeks. The competitive period as a whole showed higher mean wITL, dITL, and strain, and lower monotony than the others. However, during the competition weeks gymnasts presented the worst recovery and highest monotony scores, despite the lowest mean wITL and dITL. Negative correlation was found between dITL and TQR of the following day (r= -0.333; p&lt;0.001). The ITL and recovery profile changed between the season periods and competition weeks. The training load profile of the competitive period and competition weeks did not guarantee good recovery, especially on the weekend. More variability in load magnitude is suggested, possibly including a day off, during competitive periods and competition weeks.

A influência de Atividades Extracurriculares no Desenvolvimento de Competências Gerenciais em Grupos de Pesquisa

Este estudo visou identificar relações entre atividades extracurriculares (AE) e domínio de competências gerenciais (CG) em grupos de pesquisa (GP) brasileiros com uso da escala de domínio de CG em GP desenvolvida por Freitas e Odelius (2017), juntamente com questões relativas à contribuição de AE para o desenvolvimento de CG em GP. O questionário eletrônico foi aplicado a 528 participantes e submetido a estatísticas descritivas, análise de correlação Kendall-tau b e teste Kruskal-Wallis. A análise dos dados permitiu inferir que: 1) atuação em grupos de pesquisa, iniciação científica, extensão universitária, estágio, atuação profissional em geral, trabalho voluntário e projeto de consultoria se correlacionam com domínio de CG em todas as suas dimensões; 2) atuação em empresa júnior não se revelou capaz de influenciar significativamente o domínio de CG em GP; e 3) há diferenças significativas no domínio de CG em função do papel exercido no GP, do tipo de instituição, da área do conhecimento, da escolaridade, e do tempo de experiência com pesquisa acadêmica. Ao final, foram apresentadas conclusões, limitações e agenda para pesquisas futuras.

3-Dimensional Echocardiography and 2-D Strain Analysis of Left Ventricular, Left Atrial and Right Ventricular Function in Healthy Brazilian Volunteers.

BackgroundNew echocardiographic techniques are used in the diagnosis and prognosis of many heart diseases. However, reference values in different populations are still needed for several of these new indexes. We studied these new echocardiographic parameters in a group of Brazilians with no known cardiovascular disease.ObjectiveTo study values for new echocardiographic indexes in Brazilians without known cardiovascular disease and their correlation with age.MethodsCross-sectional study that included healthy individuals who underwent three-dimensional echocardiography (3DE) and two-dimensional speckle tracking echocardiography (STE) strain (e) analysis. Left atrial (LA) and left ventricular (LV) function were analyzed by 3DE and STE, and right ventricular (RV) function by STE. P values < 0.05 were considered significant.ResultsSeventy-seven subjects (46.7% men; 40.4 ± 10.4 years) were included. Maximum, minimum and pre-atrial contraction (pre-A) LA volumes (ml/m2) were 21.2 ± 5.5, 7.8 ± 2.5, and 11.0 ± 3.1, respectively. Peak positive global LA e (LAScd), peak negative global LA e and total global LA e (LASr) were 17.4 ± 5.2%, -13.2 ± 2.0% and 30.5 ± 5.9%, respectively. LV end-diastolic and end-systolic volumes (ml/m2) measured 57 ± 12 and 24 ± 6, and 3D LV ejection fraction measured 58 ± 6%. Global LV longitudinal, circumferential and radial e were -19 ± 2%, -19 ± 3%, and 46 ± 12%, respectively. LV torsion measured 1.6 ± 0.70 /cm. Global longitudinal RV e (RV-GLS) and RV free wall strain were -22 ± 3% and -24 ± 5%. Minimum LA and pre-A volumes, LV apical rotation, torsion and RV-GLS increased with age, while total and passive LA emptying fractions, LAScd, LASr, LV end-diastolic and end-systolic volumes decreased with age.ConclusionValues for new echocardiographic indexes in Brazilians without known cardiovascular disease and their correlation with age are presented.

Machine perfusion versus cold storage in renal preservation of deceased donors with brain death: systematic review and meta-analysis.

With the increasing use of machine perfusion in kidney transplantation, it has been observed that dynamic ischemia correlates with the improvement of organ preservation. In this context, we performed a systematic review that aimed to evaluate the efficacy of the portable machine perfusion (LifePort Kidney Transporter Machine®), used in Brazil, compared to cold storage, regarding the delayed graft function of deceased donors with brain death. Literature search was carried out in LILACS, MEDLINE via PubMed, Scopus, Clarivate Analytics, Cochrane Library, Embase, and SciELO, as well as in Google Scholar manually. The systematic review consisted only of randomized clinical trials. For meta-analysis, relative risk and odds ratio were evaluated. Eighty-six documents were identified and two papers from European and Brazilian groups were selected at the end, with eligibility criteria for meta-analysis. In these, 374 kidneys were assigned to machine perfusion and 374 kidneys were assigned to cold storage. Delayed graft function was observed in 84 and 110 patients, respectively. In meta-analysis, a risk ratio of 0.7568 (p=0.0151) and an odds ratio of 0.6665 (p=0.0225) were obtained, both with a 95% confidence interval. Machine perfusion reduced the incidence of delayed graft function of deceased donors with brain death.

Mapping of the Brazilian Groups Studying Nanocellulose

The nanocellulose is a material that has gained much attention in the recent years. So, the relevance of Brazil in this field was evaluated concerning the scientific publications in Web of Science. Next, the Brazilian groups ... | Find, read and cite all the research you need on Tech Science Press

Metrópoles brasileiras: A ação dos coletivos de comunicação contra a barbárie na retomada do espaço público

This paper aims to discuss situations such as the impossibility of free circulation in the cities, the confining and segregation of the population to peripheral areas, the lack of access to public spaces, and finally the confrontation with police violence as a way to immobilize peripheral neighborhoods, through the basic premise of the need for the real use of the city by its people, and communication as a tool of inclusion and mobilization in public space. Beginning with a brief discussion about racism and conservatism as a structural scenario of the reality of life in Brazil, we present an analysis of the communication model adopted by two Brazilian groups in the current context of public spaces’ precariousness: the Papo Reto group (Rio de Janeiro, Southest) and the Tela Firme group (Para, North). These groups share their discourse production from the peripheral areas of great urban centers, as well as the questioning of how cities have been occupied from the violence, dwelling and culture points of view. The purpose of this paper is to draw a spirit of citizenship inspired by the collectivist notion of polis and commonwealth, as a substitute to a particularistic and conservative vision of the city. We believe that these groups promote ways of urban occupation based on the will to build a more humanized and equal city which includes all kinds of affections and struggles of everyday life.

Professor José Luís Costa Lima, with an extensive and prestigious academic career, collaborated and continues to collaborate with many Brazilian research groups

Professor José Luís Costa Lima, with an extensive and prestigious academic career, collaborated and continues to collaborate with many Brazilian research groups

Year in Review 2018 by the Brazilian Gastrointestinal Tumors Group: Neuroendocrine Tumors

Year in Review 2018 by the Brazilian Gastrointestinal Tumors Group: Neuroendocrine Tumors

Opções de tratamento no câncer colorretal metastático segundo a lateralidade e o status RAS/BRAF: uma pesquisa nacional do Grupo Brasileiro de Tumores Gastrointestinais (GTG)

Opções de tratamento no câncer colorretal metastático segundo a lateralidade e o status RAS/BRAF: uma pesquisa nacional do Grupo Brasileiro de Tumores Gastrointestinais (GTG)

Year in Review 2018 by the Brazilian Gastrointestinal Tumors Group - GTG: Gastrointestinal Tumors

ABSTRACTIn 2018, there were some important trials presented over multiple international meetings. The objective of this article is to summarize the most important and practice changing studies in gastrointestinal oncology this year. Some studies in gastroesophageal, pancreatic, colorectal and anal canal have been selected, including adjuvant therapy in pancreatic cancer, immunotherapy in metastatic gastroesophageal cancer, as well as strategies in regorafenib dose escalation and the role of hyperthermic intraperitoneal chemotherapy in metastatic colorectal.

Hypertension is the underlying cause of death assessed at the autopsy of individuals.

To analyze hypertension and its relationship with the causes of death identified by the autopsy. Cross-sectional study analyzed 356 participants belonging to the Brazilian Aging Brain Study Group, over 50 years of age, autopsied at the Sao Paulo Autopsy Service between 2004 to 2014. A clinical interview was conducted with the informant of the deceased. Hypertension was defined by reporting the disease and/or use of antihypertensive medication, by the informant of the deceased. Descriptive analyzes and bivariate and multivariable associations were performed. The prevalence of hypertension was 66.2% and it was the second leading cause of death (25.6%) identified by autopsy, preceded by atherosclerosis (37.8%). The variables associated with hypertension were: female gender (OR=2.30 (1.34-3.90)); living with partner [OR=0.55 (0.32-0.92)]; Body Mass Index [OR=1.14 (1.08-1.22)] and history of diabetes [OR=2.39 (1.34-4.27)]. The prevalence of hypertension was high, and it was the second most common underlying cause of death. The gold standard for the definition of cause of death, the autopsy, shows important results, which confirmed the relevance of hypertension as a public health problem.

Revisão Ano 2018 pelo Grupo Brasileiro de Tumores Gastrointestinais - GTG: Tumores Gastrointestinais

Revisão Ano 2018 pelo Grupo Brasileiro de Tumores Gastrointestinais - GTG: Tumores Gastrointestinais

Year in Review 2018 by the Brazilian Gastrointestinal Tumors Group: Neuroendocrine Tumors

Year in Review 2018 by the Brazilian Gastrointestinal Tumors Group: Neuroendocrine Tumors