The inclusion of deaf people in the Brazilian educational system is guaranteed by the use of Brazilian Sign Language (Libras). It is important that the learning process of these students, especially when dealing with abstract concepts such as those related to Chemistry, is offered based on teaching tools that cover this language. The objective of this work was to develop a guide with signs in Libras, already existing in the literature, of chemistry laboratory materials as a didactic device aiming to promote inclusion and facilitate access to experimental chemistry teaching for deaf students. The work began with meetings to plan the preparation of the booklet and choose the most common materials in laboratory classes (analytical balance, volumetric flask, beaker, burette, Erlenmeyer flask, funnel, suction bulb, volumetric and graduated pipettes, beaker and glass clock) accompanied by searching for the corresponding sign in Libras. The guide was created considering the five Libras parameters: point of articulation, movement, facial/body expression, configuration and orientation of the hands. Therefore, the material created constitutes a facilitator and important tool for interaction between teacher and student in laboratory classes, resulting in a more dynamic and efficient teaching and learning process for the target audience.