Botanical Ingredients Research Articles

Exhaustive Analytical Profiling of Phytocompounds in Botanical Active Ingredients: Fighting the Global Prevalence of Adulterated Botanical Ingredients for Cosmetics

Traditional herbal medicine, ethnopharmacology, and evidence-based phytotherapy inspire the development of botanical active ingredients for cosmetics. Ensuring their authenticity and quality is essential in guaranteeing the safety and efficacy of cosmetic formulations. However, the industry faces challenges related to adulteration and inconsistent verification practices. Adulteration can occur at both the crude raw material stage and during processing, involving misidentification, contamination, or the addition of unauthorized substances. This review emphasizes the need for robust authentication methods, including botanical identification, genetic testing, and phytochemical/metabolomic profiling. Analytical tools such as UV/VIS spectroscopy, HPTLC, GC-MS, HPLC/UHPLC, and isotope analysis provide complementary data for detecting and addressing adulteration. Adulteration jeopardizes product safety, efficacy, regulatory compliance, and consumer trust, while dilutions or substitutions erode the intended health benefits. A standardized, comprehensive approach across the supply chain—from raw material sourcing to extract manufacturing—is critical for maintaining the integrity of botanical ingredients. Cosmetovigilance and nutrivigilance are crucial aspects of ensuring product safety and compliance. This review presents a novel perspective by highlighting that, while the pharmaceutical and nutraceutical industries have long recognized the risks of botanical adulteration, awareness in the cosmetics industry remains limited. It further integrates recent advancements in metabolomic profiling, global regulatory challenges, and the economic implications of botanical adulteration in cosmetics. Future developments in AI-driven authentication technologies may represent a promising solution for addressing evolving challenges in product safety and traceability.

Development of reference materials for dietary supplements-analytical challenges, use, limitations, and future needs.

For two decades, the National Institute of Standards and Technology (NIST) and the National Institutes of Health Office of Dietary Supplements have collaborated to develop dietary supplement-matrix reference materials. During the first decade, NIST developed over 20 botanical and non-botanical dietary supplement Standard Reference Materials (SRMs®) using multiple analytical techniques to assign values for selected marker compounds and toxic elements. In the past decade, NIST has expanded the scope of materials available, and other producers of certified reference materials (CRMs) have joined to provide a limited number of additional materials. This review describes briefly the first decade in the development of CRMs for dietary supplements, primarily botanical dietary supplement ingredients (e.g., ginkgo, green tea, saw palmetto, St. Johns' wort, botanical oils, berries, and soy) and a popular multivitamin/multimineral (MVM) SRM. We discuss the analytical challenges in producing these materials and how these materials established a model for the next generation of CRMs. The second generation of dietary supplement CRMs/RMs, consisting primarily of botanical matrices, calibration solutions, and new and replacement MVM CRMs, is discussed in greater detail including improvements based on experiences from the first decade and potential future needs and developments in this emerging reference material research sector.

Exploring the In Vitro Photoprotective Effect of a Combination of Aspalathus linearis Natural Extracts: First Steps in Developing New Technologies for Photoprotection Strategies

There is a need for new technologies in photoprotection. The negative effects of solar exposure on the skin have been amply demonstrated and there is an urgency for the development of alternative photoprotective approaches. In this respect, natural extracts represent the most interesting and promising source. Among them, Aspalathus linearis extracts appear to be an excellent candidate due to supporting evidence, their multiple beneficial biological effects, and their robust toxicological profile. Here, we first explored the photoprotective properties of two different Aspalathus linearis extracts (fermented and unfermented) individually, and then in combination, in a simplified model assessing Normal Human Dermal Fibroblast survival after UVB radiation. Surprisingly, we found the fermented extract to be more photoprotective than the unfermented one. In addition, a specific combination of the two extracts showed a synergistic effect. By HPLC and FRAP analyses, we observed that the photoprotective effect did not correlate with the amount of Aspalathus linearis main characteristic metabolites nor with the antioxidant capacity of the ingredients. Finally, an additional photoprotective effect was observed when Aspalathus linearis extracts were combined with a Polypodium leucotomos extract (Fernblock®), a well-known botanical ingredient with demonstrated photoprotection activities. Thus, this work provides a solid scientific basis for the inclusion of this technology in future topical and systemic photoprotective strategies.

Bridging regulation and practice: CJEU and Dutch case law on botanical health claims.

Even though botanicals are increasingly popular ingredients for food supplements, health claims related to their putative bene ts remain unclearly regulated. Through an analysis of EU and national case law from the Netherlands, including self-regulatory decision-making, we have studied the implications of case law on botanical health claims. Our analysis reveals that there are multiple issues related to claims on botanical-containing products: whether it should be classi ed as food or medicine; what statements should be understood as health claims; what type of evidence should underlie health claims and, more specically, botanical health claims; and how to deal with online commercial communication. The case law analysis highlights rst that a gray area will continue to exist when classifying products as foods or medicinal products, particularly when it comes to products that contain botanical ingredients. Most importantly, our study also reveals that claims-even when they are on hold, like botanical claims-need a certain scienti c foundation before they can be used on products. Finally, the courts believe that even though on-hold claims will continue to give a certain level of uncertainty for food business operators, this is not a legal but rather a regulatory issue. The findings from our case law analysis highlight that even though case law is useful in further interpretation of legislation, it does not provide any policy advancement. In the case of botanicals, a political decision regarding their substantiation is highly desired.

Nature Nano-Worriers: Phytoconstituents Loaded Lipid Nano Particles against Cancer

This review explores the potential of nanotechnology and phytomedicine in the fight against cancer. It explores the challenges posed by cancer treatment and the potential of using lipid nanoparticles for drug delivery. This article discusses the advantages of using phytochemicals as anti-inflammatory drugs and highlights the role of nanocarriers in improving their bioavailability and therapeutic effects. Different types of lipid-based nanoparticles, such as liposomes, solid lipid nanoparticles, and nanostructured lipid carriers, have been studied for their ability to trigger a response in cancer cells. This review also discusses the challenges and future directions in the field and highlights the need for further research and development to exploit the full potential of lipid nanoparticles loaded with botanical ingredients in cancer therapy.

Antioxidant content following fermentation of lemongrass for herbal beverage development.

Consumers have increasingly favoured fermented drinks due to their high content of probiotic secondary metabolites. These beverages are believed to possess the capacity to safeguard against non-communicable ailments such as coronary heart disease, cancer, diabetes, antimicrobial infections, and other dietary-related disorders. Lemongrass (Cymbopogon citratus) is a commonly used botanical ingredient in therapeutic tea production. It is renowned for its highly valuable essential oil, which has significant commercial demand. This study examines the functional content and antioxidant effects of fermented beverages derived from lemongrass. We employed the yeast Saccharomyces cerevisiae to carry out the fermentation process on the lemongrass compositions, extending the duration from t = 24 to t = 96h. We used non-fermented samples as control. This investigation identified numerous active biomolecules and polyphenols in the fermented samples, including flavonoids, tannins, cardiac glycosides, coumarins, terpenoids, steroids, saponins, and reducing sugars. After t = 24h fermentation, the radical-scavenging activity reached its maximum level of 89.1%, and the antioxidant content reached 13.06µg/ml, which is equivalent to the amount of ascorbic acid. After t = 36h fermentation, the total phenolic content reached a concentration of 237.19µg/ml, while the flavonoid content reached its peak at 55.21µg/ml after t = 72h fermentation. Lemongrass fermentation exhibits a wide range of phytochemicals and bioactive components that effectively eliminate free radicals, despite the antioxidant content fluctuation throughout the fermentation period of t = 24 to t = 96h.

Natural Modulators of Key Signaling Pathways in Skin Inflammageing.

Low-grade chronic inflammation without obvious infection is defined as "inflammageing" and a key driver of skin ageing. Although the importance of modulating inflammageing for treating skin diseases and restoring cutaneous homeostasis is increasingly being recognized. However, the mechanisms underlying skin inflammageing, particularly those associated with natural treatments, have not been systematically elucidated. This review explores the signaling pathways associated with skin inflammageing, as well as the natural plants and compounds that directly or indirectly target these pathways. Nine signaling pathways and 60 plants/constituents related to skin anti-inflammageing are discussed, exploring plant mechanisms to mitigate skin inflammageing. Common natural plants with anti-inflammageing activity are detailed by active ingredients, mechanisms, therapeutic potential, and quantitative effects on skin inflammageing modulation. This review strengthens our understanding of these botanical ingredients as natural interventions against skin inflammageing and provides directions for future research.

Safety Assessment of Wheat-Derived Ingredients as Used in Cosmetics.

The Expert Panel for Cosmetic Ingredient Safety (Panel) assessed the safety of 27 wheat-derived ingredients. Most of these ingredients are reported to function as skin conditioning agents in cosmetic products. The Panel reviewed the available data to determine the safety of these ingredients. Industry should continue to use good manufacturing practices to limit impurities that could be present in botanical ingredients. The Panel concluded that 21 wheat-derived ingredients are safe in cosmetics in the practices of use and concentration described in this safety assessment. However, the Panel also concluded that the available data are insufficient to make a determination of safety that the remaining six wheat-derived ingredients are safe under the intended conditions of use in cosmetic formulations.

Quercetin: A Promising Candidate for the Management of Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD).

Metabolic dysfunction-associated steatotic liver disease (MASLD) represents a chronic liver disease. The development of MASLD is influenced by a multitude of diseases associated with modern lifestyles, including but not limited to diabetes mellitus, hypertension, hyperlipidaemia and obesity. These conditions are often consequences of the adoption of unhealthy habits, namely a sedentary lifestyle, a lack of physical activity, poor dietary choices and excessive alcohol consumption. The treatment of MASLD is primarily based on modifying the patient's lifestyle and pharmacological intervention. Despite the absence of FDA-approved pharmacological agents for the treatment of MASLD, several potential therapeutic modalities have demonstrated efficacy in reversing the histopathological features of the disease. Among the botanical ingredients belonging to the flavonoid group is quercetin (QE). QE has been demonstrated to possess a number of beneficial physiological effects, including anti-inflammatory, anticancer and antifungal properties. Additionally, it functions as a natural antioxidant. Preclinical evidence indicates that QE may play a beneficial role in reducing liver damage and improving metabolic health. Early human studies also suggest that QE may be an effective treatment for MASLD due to its antioxidant, anti-inflammatory, and lipid-regulating properties. This review aims to summarize the available information on the therapeutic effects of QE in MASLD.

Formulation and evaluation of herbal makeup primer

The burgeoning interest in natural and sustainable beauty products has driven the development of herbal makeup primers, which combine cosmetic functionality with the benefits of botanical ingredients. This abstract explores the formulation, efficacy, and advantages of herbal makeup primers. Unlike traditional primers that often rely on synthetic chemicals, herbal primers leverage plant-based extracts known for their skin-enhancing properties, such as aloe Vera& Orange oil. These ingredients offer soothing, anti-inflammatory, and antioxidant benefits that can improve skin texture and promote a healthier complexion. The formulation of herbal makeup primers typically includes a blend of herbal extracts, essential oils, and natural emulsifiers. These components work synergistically to create a smooth base for makeup application while also addressing skin concerns such as redness, dryness, and uneven tone. Clinical and consumer studies suggest that herbal primers can enhance makeup longevity and performance, while simultaneously contributing to overall skin health. In summary, herbal makeup primers represent a significant advancement in cosmetics science by integrating the therapeutic benefits of herbal ingredients into everyday beauty routines. Their growing popularity underscores a shift towards more eco-friendly and skin-conscious beauty solutions, aligning with consumer demand for products that offer both cosmetic and therapeutic benefits. Future research and development in this field are likely to expand the range of available formulations and further validate the efficacy of these natural alternatives.

Commercialized "Smudge Sticks" Used as Incense in the Netherlands: An Inventory of Plants and Trends Behind a New Age Fashion.

Incense is essential in religious ceremonies, even in relatively new religious and spiritual movements such as New Age and Neopaganism. These garner little attention from ethnobotanists, although they trigger an international trade in wild-harvested plants. In this paper, we studied the botanical ingredients of smudge sticks (dried plant bundles burned for purification) in the Netherlands, and people's motivations to use them posing the following questions: what plant species are included in smudge sticks? what are they used for? and are exotic plants preferred over native Dutch plant species? We visited online and physical shops in Dutch cities, acquiring a total of 29 different smudge sticks containing at least 15 species. We held semi-structured interviews with 11 users, vendors, and herbal experts, and collected data from 33 questionnaires. Salvia apiana L. was most frequently found, along with North American species of the genus Artemisia. The rise of the New Age movement resulted in North American ritual plant species being easily available in (online) shops in the Netherlands and smudge sticks being used for personal protection and cleansing. Despite the smudge sticks' commercial demand, there is no data regarding the pressure on wild populations of species used in these bundles. For the preservation of these species it is crucial that scientific monitoring of their harvest is undertaken in the future.

A Narrative Review: Immunometabolic Interactions of Host-Gut Microbiota and Botanical Active Ingredients in Gastrointestinal Cancers.

The gastrointestinal tract is where the majority of gut microbiota settles; therefore, the composition of the gut microbiota and the changes in metabolites, as well as their modulatory effects on the immune system, have a very important impact on the development of gastrointestinal diseases. The purpose of this article was to review the role of the gut microbiota in the host environment and immunometabolic system and to summarize the beneficial effects of botanical active ingredients on gastrointestinal cancer, so as to provide prospective insights for the prevention and treatment of gastrointestinal diseases. A literature search was performed on the PubMed database with the keywords "gastrointestinal cancer", "gut microbiota", "immunometabolism", "SCFAs", "bile acids", "polyamines", "tryptophan", "bacteriocins", "immune cells", "energy metabolism", "polyphenols", "polysaccharides", "alkaloids", and "triterpenes". The changes in the composition of the gut microbiota influenced gastrointestinal disorders, whereas their metabolites, such as SCFAs, bacteriocins, and botanical metabolites, could impede gastrointestinal cancers and polyamine-, tryptophan-, and bile acid-induced carcinogenic mechanisms. GPRCs, HDACs, FXRs, and AHRs were important receptor signals for the gut microbial metabolites in influencing the development of gastrointestinal cancer. Botanical active ingredients exerted positive effects on gastrointestinal cancer by influencing the composition of gut microbes and modulating immune metabolism. Gastrointestinal cancer could be ameliorated by altering the gut microbial environment, administering botanical active ingredients for treatment, and stimulating or blocking the immune metabolism signaling molecules. Despite extensive and growing research on the microbiota, it appeared to represent more of an indicator of the gut health status associated with adequate fiber intake than an autonomous causative factor in the prevention of gastrointestinal diseases. This study detailed the pathogenesis of gastrointestinal cancers and the botanical active ingredients used for their treatment in the hope of providing inspiration for research into simpler, safer, and more effective treatment pathways or therapeutic agents in the field.

The benefits and challenges of treating skin with natural oils.

The term natural oil refers to a fixed (non-volatile) oil of animal or plant origin. These types of oils - in contrast to essential (volatile) oils, which are obtained by steam distillation methods of plant matter - are typically obtained from plant seeds and nuts by a mechanical pressing technique or solvent extraction. The natural movement in cosmetics of the 21st century has led to renewed interest in formulating skin care products with botanical ingredients. In this article, we discuss the benefits and caveats of natural oil treatments as moisturizing agents (as occlusives and emollients) as well as their utility in wound healing and treatment of skin diseases. We also address the paradoxical behaviour of natural oils in relation to barrier function and highlight the current state of our knowledge with respect to the use of natural oils in neonatal skin care. Finally, we provide a comparison of natural oils to conventional petroleum-based oils.

Edible Flowers in Herbal Beverage Development

Herbal beverages with nutraceutical benefits are becoming more widely acknowledged as healthy dietary choices among mindful consumers. There are numerous types of herbal beverages prepared from different morphological plant elements. Edible flowers, with their unique flavors, distinct aromas, vivid colors, and nutraceutical properties, have gained predominance among all the other botanical ingredients utilized in the formulation of herbal beverages. This review elaborates on applications of edible flowers used in the preparation of herbal teas, infusions, and a few other novel developments in herbal beverages. Although several commercialized herbal beverages have been developed with edible flowers, many other edible flowers with medicinal properties remain unexplored. The review further elaborates on the pharmacotherapeutic properties of these flowers, emphasizing their potential to be used in herbal beverage formulations. Additionally, ensuring the food safety aspects of innovative herbal beverages derived from edible flowers by addressing their potential toxicity and microbiological quality is vitally important. Insights presented here pave the way for incorporating edible flowers in herbal beverages, encouraging nutritious and health-promoting beverage development and consumption.

Formulation and evaluation of herbal scrub soap

Scrubbing Soap are the agents which are use to remove the dead cell from skin, pigmentation and blackheads, white heads and make skin glowing, smooth, soft and healthy. Scrubbing soap can be directly applied onto the skin by gently massage is recommended on application of the scrubbing soap which helps to improve blood circulation and increase oxygen supply to all surface of the skin. Due to some environmental factors many people face some problem like pigmentation and uneven skin tone. Solution for this problem is use of scrubbing soap which consist ingredients which increases cleansing, softening, moisturizing, fairness of skin. In this preparation neem, tulsi and poppy seeds are use as main active ingredients. Other ingredients like glycerin base, essential oil and rose water, ritha powder, alovera, turmeric vitamin E are use in the formulation. Thus, the developed formulation can be used as an effective scrubbing soap for using it to bear a healthy and glowing skin. Ayurvedic cosmetics are also known as the herbal cosmetics the natural content in the herbs does not have any side effect on the human body most herbal supplement are based on several botanical ingredients with long histories of traditional or folk medicine usage. Among the numerous botonical ingredients available in the market today. Numerous chemical toxins microorganism present in the atmosphere may cause chemical infection and damage to skin cosmetics alone are not sufficient to take care of skin and body parts. Neem (Azadirachta indica) tree has attracted worldwide prominance owing to its wide range of medicinal properties, neem leaves and its constituents have been demonstrated to exhibit anti–inflammatory, antihyperglycemic, antiulcer, antimalarial, antifungal, antibacterial, antimutagenic and anticarcinogenic properties. This study was conducted to evaluate the effect of aqueous, ethanolic and ethyl acetate extract from neem leaves.

Use of Botanical Ingredients: Nice Opportunities to Avoid Premature Oxidation of NABLABs by Increasing Their ORAC Values Strongly Impacted by Dealcoholization or Pasteurization

Even when fresh, non-alcoholic, and low-alcoholic beers (NABLABs) exhibit significant staling defects due to premature oxidation. In this study, the antioxidant power of eleven fresh commercial NABLABs was assessed by means of three different assays: the oxygen radical absorbance capacity (ORAC), the linoleic acid-induced oxidation (TINH), and the indicator time test (ITT). Only the first two assays, both involving radicalar degradations initiated by AAPH, were found to correlate with each other. NABLABs displayed lower ORAC values than conventional beers (on average, 6127 μmol eq. Trolox/L), except for three samples made with special-colored malts or dry-hopped. Dealcoholization was the step with the greatest impact on the ORAC value (up to a 95% loss) and on flavan-3-ols, sotolon, and polyfunctional thiols, while pasteurization strongly affected color, TBA, and Strecker aldehydes. ORAC assays applied to hop, alternative cereals, and various botanical ingredients indicated that mashing with red sorghum, dry hopping/spicing, and wood maturation could bring the antioxidant power of a NABLAB close to those of conventional beers. With an ORAC value not reached by any other tested botanical ingredient (5234 µmol eq. Trolox/g), African Vernonia amygdalina leaves (traditionally used for Rwandan Ikigage beers) emerged here as the best candidate.

The undisclosed presence of excipients and diluents in botanical extracts

This article discusses the composition and labeling of botanical extracts, with particular focus on the sale of botanical dietary ingredients and dietary and food supplement products. Published literature and reports from analysts suggest that some commercial extracts in the dietary and food supplement supply chain may contain little or none of the declared plant or plant extract. Experts in dietary supplement analysis have determined that this occurs mainly because of two schemes used by deceptive suppliers. First, certain suppliers excessively dilute native plant extracts with undeclared amounts of excipients and are ambiguous in disclosing the plant-to-extract ratio. Second, whole herbs are extracted to obtain specific fractions or compounds that are considered to be therapeutically beneficial and are provided to select markets. The marc (leftover or spent biomass) may then be re-sold without disclosure that it is pre-extracted material.

Development of Certified Reference Material Solutions for Phytochemicals in Ginger and Kava.

Dietary supplements derived from botanicals are commonly consumed and investigated in biomedical studies for their potential health benefits. Accurate identification and quantification of key chemical constituents from botanical ingredients is necessary for consistent product preparations and reproducible research results. Manufacturers need quantitative reference materials of the chemical constituents of interest to verify the content of ingredients and products. The rigor and reproducibility of biomedical research is enhanced through thorough characterization of the interventions used in mechanistic, clinical, and safety investigations. Quantitative reference materials enable reliable product quality assessments and reproducible research results. Solution-based certified reference material (CRM) mixes were developed as calibrants for phytochemicals in ginger and kava. The kava CRM contained yangonin, desmethoxyyangonin, dihydrokavain, DL-kavain, methysticin, dihydromethysticin, flavokawain A, flavokawain B, and flavokawain C. The ginger CRM contained 6-gingerol, 8-gingerol, 10-gingerol, 6-shogaol, 8-shogaol, and 10-shogaol. Each phytochemical was sourced as an isolated compound and assigned a purity factor by a mass balance approach accounting for residual impurities. The solution standard mixes were formulated by gravimetric addition of each phytochemical incorporating the purity factor and diluting with acetonitrile to the target concentrations of 500 µg/mL for the gingerols and shogaols, 250 µg/mL for the kavalactones, and 25 µg/mL for the flavokawains. The concentration accuracy of each component in the solution mixes was analytically verified by ultra high performance liquid chromatography with ultraviolet detection (UHPLC-UV) assay comparison to an independently prepared calibration solution. Each component in the ginger and kava CRMs were within 5 and 7% of the target concentrations, respectively. Homogeneous kava and ginger phytochemical solution mixes were produced with accurate constituent concentrations and demonstrated good stability over 2 years. These solution mixes were launched as commercially available CRMs. These mixes can be used as accurate concentration stock solutions to prepare calibrators and controls for botanical dietary supplement product testing and standardization.

Compound collagen peptide powder improves skin photoaging by reducing oxidative stress and activating TGF-β1/Smad pathway.

Fish collagen peptide (FCP) has been extensively investigated as a natural product that can combat photoaging; however, its efficacy is limited by its singular composition. Compound collagen peptide powder (CCPP) is a novel functional food formulation that exhibits photoprotective properties and comprises FCP and a blend of natural botanical ingredients. The objective of this study was to investigate the efficacy of CCPP and its molecular mechanism. CCPP had a low molecular weight, facilitating its efficient absorption, and was abundant in amino acids, total polyphenols, and total flavonoids. The results of invivo studies demonstrated that CCPP exhibited significant efficacy in reducing skin wrinkles, enhancing the contents of water and oil in the skin, and ameliorating histopathological alterations in mice. The results of invitro studies demonstrated that CCPP effectively mitigated photoaging in human skin fibroblasts by attenuating oxidative stress and promoting extracellular matrix (ECM) synthesis. Moreover, we clearly demonstrated that the TGF β1/Smad pathway was involved in the promotion of ECM synthesis and cell proliferation by CCPP in human skin fibroblasts. These findings suggest that, compared with single collagen, CCPP has a more comprehensive range of antiphotoaging properties.

Nutraceuticals known to promote hair growth do not interfere with the inhibitory action of tamoxifen in MCF7, T47D and BT483 breast cancer cell lines.

Hair loss/thinning is a common side effect of tamoxifen in estrogen receptor (ER) positive breast cancer therapy. Some nutraceuticals known to promote hair growth are avoided during breast cancer therapy for fear of phytoestrogenic activity. However, not all botanical ingredients have similarities to estrogens, and in fact, no information exists as to the true interaction of these ingredients with tamoxifen. Therefore, this study sought to ascertain the effect of nutraceuticals (+/- estrogen/tamoxifen), on proliferation of breast cancer cells and the relative expression of ERα/β. Kelp, Astaxanthin, Saw Palmetto, Tocotrienols, Maca, Horsetail, Resveratrol, Curcumin and Ashwagandha were assessed on proliferation of MCF7, T47D and BT483 breast cancer cell lines +/- 17β-estradiol and tamoxifen. Each extract was analysed by high performance liquid chromatography (HPLC) prior to use. Cellular ERα and ERβ expression was assessed by qRT-PCR and western blot. Changes in the cellular localisation of ERα:ERβ and their ratio following incubation with the nutraceuticals was confirmed by immunocytochemistry. Estradiol stimulated DNA synthesis in three different breast cancer cell lines: MCF7, T47D and BT483, which was inhibited by tamoxifen; this was mirrored by a specific ERa agonist in T47D and BT483 cells. Overall, nutraceuticals did not interfere with tamoxifen inhibition of estrogen; some even induced further inhibition when combined with tamoxifen. The ERα:ERβ ratio was higher at mRNA and protein level in all cell lines. However, incubation with nutraceuticals induced a shift to higher ERβ expression and a localization of ERs around the nuclear periphery. As ERα is the key driver of estrogen-dependent breast cancer, if nutraceuticals have a higher affinity for ERβ they may offer a protective effect, particularly if they synergize and augment the actions of tamoxifen. Since ERβ is the predominant ER in the hair follicle, further studies confirming whether nutraceuticals can shift the ratio towards ERβ in hair follicle cells would support a role for them in hair growth. Although more research is needed to assess safety and efficacy, this promising data suggests the potential of nutraceuticals as adjuvant therapy for hair loss in breast cancer patients receiving endocrine therapy.