Bone Assemblages Research Articles

The biomechanical implications of lacunar and perilacunar microarchitecture on microdamage accumulation in cortical Bone

Purpose This study aims to explore how the microarchitectural features of lacunae and perilacunar zones impact the biomechanics of microdamage accumulation in cortical bone, crucial for understanding bone disorders' pathogenesis and developing preventive measures. Methods Utilizing the phase-field finite element method, the study analyzed three bone unit models with varying microarchitecture: one without lacunae, one with lacunae, and one including perilacunar zones, to assess their effects on cortical bone's biomechanical properties. Results The presence of lacunae was found to increase microcrack initiation risk, acting as nucleation points and accelerating microcrack propagation. Proximity to Haversian canals exacerbated stress concentration, speeding microdamage progression. Conversely, perilacunar zones mitigated both initiation and propagation. An elevated critical energy release rate correlated with slower crack growth and reduced damage severity. Conclusions The research sheds light on the intricate mechanisms governing microcrack behavior in compact bone, highlighting the significant role of bone's microarchitectural features in its biomechanical response to microdamage. These insights are valuable for the development of strategies to prevent and treat bone-related disorders.

Bone tools, carnivore chewing and heavy percussion: assessing conflicting interpretations of Lower and Upper Palaeolithic bone assemblages.

The use of bone tools by early humans has provided valuable insights into their technology, behaviour and cognitive abilities. However, identifying minimally modified or unshaped Palaeolithic osseous tools can be challenging, particularly when they are mixed with bones altered by natural taphonomic processes. This has hampered the study of key technical innovations, such as the use of bones, antlers and teeth as hammers or pressure-flakers to work (knap) stone tools. Bones chewed by carnivores can resemble osseous knapping tools and have sometimes been mistaken for them. In this paper, we review recent advances in the study of osseous knapping tools with a focus on two Palaeolithic sites in the UK, the Acheulean Horse Butchery Site at Boxgrove and the Magdalenian site of Gough's Cave, where knapping tools were mis-attributed to carnivore chewing. These osseous knapping tools are investigated using microscopy, high-resolution imaging and comparisons with experimental knapping tools. This allows for new insights into human behaviour at these sites and opens fresh avenues for future research.

Русь и Орда: возможности реконструкции экономических процессов методами археозоологии

Our analysis of archaeozoological collections found on archaeological sites allowed to build a complex picture of the meat diet of medieval settlements in Eastern Europe and to reconstruct the structure of meat supply in Eastern European towns and villages in forest, forest-steppe and steppe areas, as well as to determine the similarities and differences in this structure across different landscapes and cultural zones. We found that the dominant meat in the diet of the medieval population in all studied landscapes, regardless of settlement form was beef. For Rus’ towns, which existed for several centuries, we identified the tendency to increase the share of beef in the meat diet from the early (12th century) to the latest (16th century and later) periods. Beef also dominated the meat consumption of townspeople in the Golden Horde in the 13th–14th centuries.
 The study shows that cattle were raised in rural settlements and regularly delivered to the towns for hides and meat. Nomadic cattle, such as sheep and horses were delivered to the towns and slaughtered episodically to produce hides. In the artisanal quarters of the towns, our archaeozoological research registered accumulations of domestic ungulate bones with a specific taxonomic and anatomical set – the remains of large-scale slaughter of nomadic and rural livestock. These quarters also did the primary processing of hides – our study found clear markers of processing industries in archaeozoological collections. The study of isotopes (δ13C and δ15N) in more than 150 bone samples of domestic ungulates of forest and steppe areas proved the feasibility of using 13C isotope tags to capture the status of domestic ungulates: local and outsider; 15N isotope signals describe the contribution of farmed fodder to the diet of specific animals. We managed to discover the movement of nomadic steppe cattle from the arid zone for slaughtering and hides to the forest-steppe Bolgar town, and to record the fact of cattle exchange between steppe and forest territories, as well as between different regions of medieval Rus’.

The use and treatment of freshwater mussels in the Northeastern European lowlands and the Eastern Baltics

Freshwater shells appear repeatedly in animal bone assemblages at Late Bronze Age settlements. This is not only the case in the inland, but also in regions fairly close to the Baltic Sea shore. Significant amounts of freshwater mussels are known especially from Polish and Northeastern German fortified settlements as well as in the Lithuanian lowlands. Several large shell finds from Late Bronze Age fortified settlements in these regions have been published recently, shedding new light on the use of aquatic resources. The majority of the freshwater mussels found belong to the Unio type, whose occurrence is particularly common in contexts of the Lusatian culture. Apparently, these mussels were intentionally selected for certain purposes. However, new excavations in Lithuania show that these selection processes can be observed over a much larger area than previously assumed. This article aims to present a brief overview of freshwater mussel gathering and consumption, analyzing such behavior in a wider context, with a focus on the consumption of mussels by Late Bronze Age communities in specific regions of Northeastern Europe. The significance of mussels as a resource is placed in a larger spatial context based on analogue finds.

Alternation between humans and carnivores in the occupations of the Mousterian site of Sopeña rock-shelter (Asturias, Spain)

The Sopeña rock-shelter is a Palaeolithic site located in the northern slopes of the Cantabrian mountain range of northern Spain, facing the Bay of Biscay, in the Principality of Asturias. The shelter overlooks the Güeña River, which is a tributary to the Sella River. Excavations there yielded a long stratigraphic and archaeological sequence of episodes of human occupation, ranging from the later millennia of the Middle Palaeolithic -until the local disappearance of Neanderthals c. 42,630 ± 600 years ago, to the initial stages of the Upper Palaeolithic, which marks the local arrival of modern Homo sapiens sapiens, and an important Gravettian sequence. All the Sopeña levels yielded important accumulations of both lithic and faunal remains. The Mousterian appears, from top to bottom, in Sopeña levels XII, XIII, XIV, XV and XVI. Our study of the animal remains shows that at Level XV Neanderthal activity is very intense and responsible for the accumulation and modification of most of the bone remains (Yravedra et al., under review), while, as described below, this is not the case for levels XII, XIII and XIV, where carnivores were more involved in the accumulation and modification of animal remains. In this paper, we analyze the alternation between human and carnivore activity in the formation of the bone assemblages in the later Mousterian levels of Sopeña, on the eve of the arrival of anatomically modern humans in the Cantabrian region c. 40.000 years ago.

Water, gravity, and trophic dynamics in the Misiam palimpsest, Olduvai Gorge

Misiam is a modern leopard-generated wildebeest bone accumulation located on the slope of a steep ravine within Olduvai Gorge (Tanzania). We present a taphonomic assessment of how leopard feeding behaviour shaped the bone assemblage, accumulated during the wet season, as well as contextualising the extent of secondary hyaenid scavenging. The spatial formation of this taphonomic palimpsest has resulted from the temporal sequence of interactions with the bone remains. The impact of additional agents and processes—such as fungi, insects, weathering, and, most importantly, gravitational displacement driven by water flows generated during the rainy season—adds to the complexity of this locality. This last factor may have played a decisive role in the density-mediated osteological attrition documented in the bone assemblage, perhaps to an even greater extent than hyaenid activity. Given the critical importance for analogical studies of documenting all relevant taphonomic factors at work in modern assemblages, our perspective allows for a comprehensive interpretive assessment of the site. The Misiam surface assemblage, a leopard-generated bone accumulation palimpsest with multiple taphonomic agents and processes at work within a short time span, has therefore significant implications for nuancing or complementing the interpretation of Early Pleistocene localities, such as FLK-North, FLK-Zinj or TK in Olduvai (Tanzania), Ubeidiya (Israel) or Barranco León in Orce (Spain), among others.

Remains of fill and floor? contextual taphonomy in a semi-subterranean structure in the hyperarid coast of the Atacama Desert, Chile

Architectural features and shell-matrix sites are one of the most challenging records for archaeological interpretation, due to their intricate stratification and complex depositional paths. In the hyperarid coast of the Atacama Desert, hunter-gatherer-fisher developed standardized stone-built architectural features mostly built on or directly related to shell-matrix deposits during the Late Archaic period (5700 – 4000 cal. years B.P.). These structures have been usually interpreted as sedentary villages or long-term campsites. These functional interpretations are based primarily upon intrasite variability, lacking systematic site formation analysis and chronostratigraphy. As a response to concerns arising from limited interpretive approaches, we propose a detailed taphonomic study of bone assemblages from a stone-built funerary structure of the Zapatero site (∼25°S, Taltal, Chile). We recorded macroscopic taphonomic modification features on mammal and avian bone remains (n = 1776) from five stratigraphic units within the structure. Our results attest that despite some similarities between strata, key differences in overall proportions suggest different taphonomic pathways. This variability within the sample is interpreted as a re-deposition of bone refuse from previous occupations as structural filling, intentionally deposited previous to the abandonment of the structure, therefore not directly related to the occupation of the architectonic feature. Finally, we highlight the role that relatively simple zooarchaeological and taphonomical comparisons intra-site, might shed light on exceptional site formation processes on short timescales, emphasizing why we must consider this sort of data even in low relative abundances in order to interpret taphonomic history pathways and depositional practices.

The dik-diks of Guli Waabayo: Late Pleistocene net-hunting and forager sociality in eastern Africa

Net-hunting is closely linked to organized labor and hunter-gatherer cooperation in many world regions. At the Rifle Range Site (RRS) in southern Somalia, scholars have argued that Later Stone Age (LSA) foragers developed specialized dwarf antelope hunting strategies—possibly using communal net-drives—to facilitate developing concepts of territoriality around resource-rich inselberg environments during a wet period in the early and middle Holocene. Unfortunately, a lack of radiocarbon dates and faunal data limited detailed zooarchaeological perspectives on changing hunting patterns at the site. The large and well-dated dwarf antelope bone assemblage (1263 specimens) from nearby Guli Waabayo (GW) rock shelter, on the other hand, provides an opportunity to explore proposed relationships between net-hunting and LSA social and economic reorganization in southern Somalia ~ 26–6 thousand years ago (ka). Consistently high dik-dik frequencies (55.2–71.9%) and mortality profiles comprised of individuals from all age groups throughout the sequence do not support previous arguments associating specialized dwarf antelope hunting with territoriality and Holocene climatic amelioration at RRS. Instead, they suggest that LSA foraging groups regularly hunted dik-dik (genus Madoqua) using nets over a ~ 20,000-year period beginning as far back as the arid Marine Isotope Stage 2, 29–14.5 ka. Findings from this study complement recent arguments for greater economic variability in Late Pleistocene eastern Africa and push discussions of forager social change further back in time than previously considered.

The bird remains from La Crouzade Cave (Gruissan, Aude): The mixed origin of a Middle Palaeolithic bone accumulation

The bird remains from La Crouzade Cave (Gruissan, Aude): The mixed origin of a Middle Palaeolithic bone accumulation

Multi-purpose pots: Reconstructing early farmer behaviour at Lydenburg Heads site, South Africa, using organic residue analysis

About 2000 years ago, Bantu-speaking people introduced an agro-pastoral lifeway into southern Africa and lived in northern and eastern lowland parts of the region by 700 CE. Also defined as the ‘southern African Iron Age’, pottery is the most common and diagnostic artefact category. The soil chemistry of several farming sites resulted in poor faunal preservation and especially within Mpumalanga Province, South Africa, the possibilities for dietary reconstructions based on bone assemblages is very limited. Furthermore, the majority of ceramic research on early farming communities (ca. 200–900 CE) has focused on typology and ethnoarchaeological interpretations. This study represents the first application of Organic Residue Analysis (ORA) applied to southern African early farming pottery to gain a deeper understanding of past human behaviour and subsistence patterns. We investigate the potential and evaluate future prospects of applying this method to southern African early farming pottery. A combined lipid biomarker (GC-MS) and compound specific isotope approach (GC-C-IRMS) was applied to pottery sherds (n = 40) from the Lydenburg Heads site (LHS), dating to the 7th century CE (Mzonjani facies) and 9th to 11th centuries CE (Doornkop facies), to test for lipid preservation, trace possible vessel use and dietary changes through time and embed the research data within the faunal and botanical record available. Our study provides first evidence for the processing of dairy products by early farmers in southern Africa. Both facies imply multi-purpose functionality of the ceramics tested, demonstrated by the mixing of animal fats and plant oils. The obtained ORA data from LHS confirms the processing of ruminant carcass fats within both occupation facies, likely representing domestic animal fats, as well as the processing of wild non-ruminant carcass fats within the earlier Mzonjani facies. The identification of ricinoleic acid in two vessels of the Doornkop facies, provides evidence for potential medicinal use. Preliminary data also indicate that dung could have been involved in different forms of pottery sealing and/or mending during both occupation facies. In this study, we begin to explore biomolecular evidence for dietary choices made by early farmers in southern Africa, shedding light on previously unexplored key products.

Taphonomic data from the transitional Aurignacian of El Castillo cave (Spain) reveals the role of carnivores at the Aurignacian Delta level

Palaeolithic sites are complex palimpsests due to the recurrent presence of human groups and carnivores through time, as documented in several European caves and rock shelters. Within these contexts, it can be challenging to identify the main contributors to bone accumulations and interpret human subsistence strategies and the role of carnivores. Consequently, taphonomic analyses are crucial for distinguishing the origins of these occupation episodes. One example of alternating occupations is found at El Castillo cave (Cantabria, Spain), specifically in the so-called Transitional Aurignacian level (∼46-42 ka uncal BP). According to a recent study conducted at this level (Luret et al., 2020), the human groups had a hunting specialisation focused on red deer, while carnivores had little influence on the assemblage. However, these interpretations were based only on the faunal assemblage from the recent excavations (1980–2011). A previous excavation phase was conducted on this site from 1910 to 1914, and after that, the faunal remains recovered were separated across several institutions, lacking a complete archaeozoological and taphonomic analysis of the whole level. In this work, a new taxonomic assessment and, for the first time, a taphonomic study of the macrofauna recovered in the Aurignacian Delta level during the initial excavations, stored at Museo Nacional y Centro de Investigación de Altamira (Cantabria, Spain), is carried out. This work provides new information about the human and carnivore groups that occupied El Castillo, constructing new hypotheses to interpret this relevant archaeological level.

A new vertebrate assemblage from the Matute Formation of the Cameros Basin (Ágreda, Spain): implications for the diversity during the Jurassic/Cretaceous boundary

The Ribota site (Ágreda, Soria, Spain) is a new locality in the Matute Formation (Tithonian–Berriasian) composed of several carbonate layers, outstandingly rich in macrovertebrate remains. Fossils show an unusual replacement of the original bioapatite by quartz, and are found as positive reliefs protruding from lacustrine limestone beds. This type of conservation has allowed the identification of around one hundred vertebrate bone accumulations in an outcrop of more than 10 hectares. Osteichthyans (articulated partial skeletons, cranial material, and isolated postcranial bones and scales), crocodylomorphs (disarticulated cranial material, isolated teeth, vertebrae and osteoderms), turtles (partial carapaces and plastra, but also isolated plates) and pterosaurs (cranial and appendicular elements) have been identified. Around 80 specimens have been collected and a preliminary study of part of the collection (35 specimens) has allowed the identification of at least 5 different taxa: Halecomorphi indet., Neoginglymodi indet., Goniopholididae indet., Testudinata indet., and Pterodactyloidea indet. This new site represents one of the few sites from this time interval preserved in a fully lacustrine environment, so these vertebrate assemblages are unique and composed of different animals that presumably lived around and within the lake. They are dominated by aquatic and amphibian vertebrates and was formed by attrition in this lacustrine environment, possibly far from the lake shoreline. These macrovertebrate assemblages provide new data about the diversity in the faunal ecosystems from the Jurassic/Cretaceous transition of the Iberian Basin Rift System.

Human-beaver Engagements Seen Through Multiperiod Settlement Sites at Rautalampi, Hämeenniemi and Kitee, Hiidenniemi, Finland

The aim of this article is to investigate the relationship between beavers and humans in the prehistoric times. This is studied through animal bone assemblages excavated from two multi-period settlement sites in Northern Savonia and North Karelia, Finland, and is supplemented with ethnographic and folklore material. The theoretical framework uses perspectives from social zooarchaeology, relational ontology and multispecies archaeology and the research questions are answered with zooarchaeological analysis, age estimates and beaver ethology. This study shows that the hunted beavers were adults who could have established their own colonies, modified the landscape to suit their needs and had their first litter. Beavers had different ways of being, engaging and being present in a world that sometimes led to direct and indirect encounters between humans and beavers. The hunters had knowledge that based on the behaviour of beavers, and they used it to find the animals to engage with them.

Distinct modes and intensity of bird exploitation at the dawn of agriculture in the Upper Euphrates and Tigris River basins

Early Neolithic avifaunas excavated at the sites of Göbekli Tepe and Gusir Höyük illustrate two entirely different modes of bird exploitation in south-eastern Anatolia during the 10th and 9th millennia BCE, which prompted us the re-evaluation of other substantial bird bone assemblages from contemporaneous sites in the upper basins of the Euphrates and Tigris rivers. We thus compared the range and diversity of species captured, seasonal aspects of bird hunting, and types of habitats exploited by Near Eastern communities of foragers at the dawn of agriculture. Fowling practices in the Upper Euphrates catchment aimed at multiple avifaunal resources in varied habitats explaining high taxonomic diversity in the respective assemblages. Overall, the proportions of birds, mammals and fish in the diet were similar in all sites. In contrast, meat diet in the Upper Tigris River basin proved less culturally uniform. The contribution of birds, fish and mammals to the diet varied significantly between sites. Our study also showed that avifaunal diversity was very low at some sites in the Tigris basin suggesting an overall limited interest and time commitment to this economic activity there. An intensification of bird hunting in autumn and winter was observed in both areas.

Immunometabolic capacities of nutritional fatty acids in regulation of inflammatory bone cell interaction and systemic impact of periodontal infection.

Novel preventive strategies in periodontal disease target the bacterial-induced inflammatory host response to reduce associated tissue destruction. Strategies focus on the modulation of tissue-destroying inflammatory host response, particularly the reduction of inflammation and promotion of resolution. Thereby, nutrition is a potent immunometabolic non-pharmacological intervention. Human studies have demonstrated the benefit of olive oil-containing Mediterranean-style diets (MDs), the main component of which being mono-unsaturated fatty acid (FA) oleic acid (OA (C18:1)). Hence, nutritional OA strengthened the microarchitecture of alveolar trabecular bone and increased circulating pro-resolving lipid mediators following bacterial inoculation with periodontal pathogen Porphyromonas gingivalis, contrary to saturated FA palmitic acid (PA (C16:0)), which is abundant in Western-style diets. Additionally, the generalized distribution of inflammatory pathway mediators can occur in response to bacterial infection and compromise systemic tissue metabolism and bone homeostasis distant from the side of infection. Whether specific FA-enriched nutrition and periodontal inoculation are factors in systemic pathology that can be immune-modulatory targeted through dietary substitution is unknown and of clinical relevance. Normal-weight C57BL/6-mice received OA-or PA-enriched diets (PA-ED, OA-ED, PA/OA-ED) or a normal-standard diet (n=12/group) for 16 weeks and were orally infected with P. gingivalis/placebo to induce periodontal disease. Using histomorphometry and LC-MS/MS, systemic bone morphology, incorporated immunometabolic FA-species, serological markers of bone metabolism, and stress response were determined in addition to bone cell inflammation and interaction in vitro. In contrast to OA-ED, PA-ED reduced systemic bone microarchitecture paralleled by increased lipotoxic PA-containing metabolite accumulation in bone. Substitution with OA reversed the bone-destructive impact of PA, which was accompanied by reduced diacylglycerols (DAG) and saturated ceramide levels. Further, PA-associated reduction in mineralization activity and concomitant pro-inflammatory activation of primary osteoblasts were diminished in cultures where PA was replaced with OA, which impacted cellular interaction with osteoclasts. Additionally, PA-ED increased osteoclast numbers in femurs in response to oral P. gingivalis infection, whereas OA-ED reduced osteoclast occurrence, which was paralleled by serologically increased levels of the stress-reducing lipokine PI(18:1/18:1). OA substitution reverses the bone-destructive and pro-inflammatory effects of PA and eliminates incorporated lipotoxic PA metabolites. This supports Mediterranean-style OA-based diets as a preventive intervention to target the accumulation of PA-associated lipotoxic metabolites and thereby supports systemic bone tissue resilience after oral bacterial P. gingivalis infection.

Liposome-encapsulated zoledronate increases inflammatory macrophage population in TNBC tumours

BackgroundTumour associated macrophages (TAMs) are important players in breast tumour progression and metastasis. Clinical and preclinical evidence suggests a role for zoledronate (ZOL) in breast cancer metastasis prevention. Further, zoledronate is able to induce inflammatory activation of monocytes and macrophages, which can be favourable in cancer treatments. The inherent bone tropism of zoledronate limits its availability in soft tissues and tumours. In this study we utilised an orthotopic murine breast cancer model to evaluate the possibility to use liposomes (EMP-LIP) to target zoledronate to tumours to modify TAM activation. MethodsTriple-negative breast cancer 4T1 cells were inoculated in the 4th mammary fat pad of female Balb/c mice. Animals were divided according to the treatment: vehicle, ZOL, EMP-LIP and liposome encapsulated zoledronate (ZOL-LIP). Treatment was done intravenously (with tumour resection) and intraperitoneally (without tumour resection). Tumour growth was followed by bioluminescence in vivo imaging (IVIS) and calliper measurements. Tumour-infiltrating macrophages were assessed by immunohistochemical and immunofluorescence staining. Protein and RNA expression levels of inflammatory transcription factors and cytokines were measured by Western Blotting and Taqman RT-qPCR. ResultsLiposome encapsulated zoledronate (ZOL-LIP) treatment suppressed migration of 4T1 cell in vitro. Tumour growth and expression of the angiogenic marker CD34 were reduced upon both ZOL and ZOL-LIP treatment in vivo. Long-term ZOL-LIP treatment resulted in shift towards M1-type macrophage polarization, increased CD4 T cell infiltration and activation of NF-κB indicating changes in intratumoural inflammation, whereas ZOL treatment showed similar but non-significant trends. Moreover, ZOL-LIP had a lower bisphosphonate accumulation in bone compared to free ZOL. ConclusionResults show that the decreased bisphosphonate accumulation in bone promotes the systemic anti-tumour effect of ZOL-LIP by increasing inflammatory response in TNBC tumours via M1-type macrophage activation.

Identifying the unidentified fauna enhances insights into hominin subsistence strategies during the Middle to Upper Palaeolithic transition

Understanding Palaeolithic hominin subsistence strategies requires the comprehensive taxonomic identification of faunal remains. The high fragmentation of Late Pleistocene faunal assemblages often prevents proper taxonomic identification based on bone morphology. It has been assumed that the morphologically unidentifiable component of the faunal assemblage would reflect the taxonomic abundances of the morphologically identified portion. In this study, we analyse three faunal datasets covering the Middle to Upper Palaeolithic transition (MUPT) at Bacho Kiro Cave (Bulgaria) and Les Cottés and La Ferrassie (France) with the application of collagen type I peptide mass fingerprinting (ZooMS). Our results emphasise that the fragmented component of Palaeolithic bone assemblages can differ significantly from the morphologically identifiable component. We obtain contrasting identification rates between taxa resulting in an overrepresentation of morphologically identified reindeer (Rangifer tarandus) and an underrepresentation of aurochs/bison (Bos/Bison) and horse/European ass (Equus) at Les Cottés and La Ferrassie. Together with an increase in the relative diversity of the faunal composition, these results have implications for the interpretation of subsistence strategies during a period of possible interaction between Neanderthals and Homo sapiens in Europe. Furthermore, shifts in faunal community composition and in carnivore activity suggest a change in the interaction between humans and carnivores across the MUPT and indicate a possible difference in site use between Neanderthals and Homo sapiens. The combined use of traditional and biomolecular methods allows (zoo)archaeologists to tackle some of the methodological limits commonly faced during the morphological assessment of Palaeolithic bone assemblages.

A deep learning-based taphonomical approach to distinguish the modifying agent in the Late Pleistocene site of Toll Cave (Barcelona, Spain)

ABSTRACT One of the most widely used methods to associate lithic tools and bone assemblage in archaeological sites is the identification of cut-marks. However, the identification of these marks is still problematic in some localities on account of the similarities with the modifications generated by non-human processes, including biostratinomic and post-depositional bone surface modifications. Toll Cave (Barcelona, Spain), with chronologies between 47.310 BP and 69.800 BP, is one of the case studies where the cut-marks could easily be confused with abundant grooves generated by the dragging of sedimentary particles (e.g. trampling), but also with the scores produced by carnivores. In this work, we present the results obtained from applying Deep Learning (DL) models to the taphonomic analysis of the site. This methodological approach has allowed us to distinguish the bone surface modifications with 97.5% reliability. We show the usefulness of this technique to help solve many taphonomic equifinality problems in the archaeological assemblages, as well as the need to implement new approaches to eliminate subjectivity in the descriptions of bone damage and make more accurate inferences about the past.

Subsistence strategies during the Gravettian in the rock shelter of La Viña (Asturias, N Spain)

The rock shelter of La Viña, located on the northern Atlantic coast of the Iberian Peninsula, contains a rich archaeological sequence dated from the Mousterian to the Magdalenian. This site preserves an extended Gravettian occupation where a diversified assemblage of fauna, lithic and bone tools has been attested. In this paper, we undertake the archaeozoological and taphonomic study of the macromammals found in the Gravettian levels X and IX of the Western Sector, recovered during the campaigns directed by Fortea between 1987 and 1992. The taphonomy indicates a high presence of fire-altered bone elements and butchery marks related to ungulate skinning and disarticulation activities, with a low presence of carnivore modifications, being Cervus elaphus, followed by Rupicapra rupicapra and Capra pyrenaica the most common taxa, therefore confirming the anthropogenic origin of the bone assemblage. Given the high fragmentation of the infantile remains identified, a biomolecular study by Zooarchaeology by Mass Spectrometry (ZooMS) was carried out to increase the taxonomic data and unravel the hunting strategies and site seasonality. Proteomic results revealed a high presence of newborn red deer and ibex, thus suggesting females herd captures during late spring/early summer.

Evaluation of bone mineral density and bone turnover in children on anticoagulation.

Childhood and adolescence are critical periods of bone mineral acquisition. Children on anticoagulation (AC) might have an increased risk for reduced bone mineral density (BMD). Risk factors for impaired bone accumulation include chronic diseases, immobility, and medication. Vitamin K (VK) deficiency reflected by undercarboxylated osteocalcin levels (ucOC) has been identified as a predictor of osteoporosis and fractures. Data on bone health in children under AC are sparse. To evaluate BMD in children on AC and characterize the risk factors of low BMD, including VK and Vitamin D (VD) status. Single-center cross-sectional study of clinical, biochemical, and densitometric parameters. Assessment of VK surrogate parameters included ucOC and matrix gla protein (MGP). A total of 39 children (4-18 years; 12 females) receiving AC were included, 31 (79%) on VK antagonists and 8 (21%) on direct oral anticoagulants. Overall, BMD was decreased for both the lumbar spine (LS; -0.7SDS) and total body less head (TBLH; -1.32SDS) compared with pediatric reference data. Significant associations were found between early pubertal development and TBLH-BMD, and between BMI and LS-BMD. VK surrogate parameters were highly related to patients' age and pubertal development. Neither serum parameters nor AC-related factors predicted BMD. VD was detected in 10/39 patients with lower values during puberty. Our data indicate BMD reduction in pediatric patients on AC. Although AC-related factors did not predict reduced BMD, low BMI and pubertal stages represented important risk factors. Awareness of risk factors for low BMD and high prevalence of VD deficiency during puberty could contribute to the improvement of bone health in this vulnerable patient group.