Body Weight Status Research Articles

The Effect of Premedication with Metoprolol on the Surgical Field during Endoscopic Stapedectomy.

Otosclerosis is one of the most frequent causes of conductive hearing loss in adults. Surgical treatment is considered to be the gold standard for this disease. Endoscopic stapedectomy is a new approach for the treatment of otosclerosis that requires a bloodless field. To determine whether the routine use of β blockers as a premedication can improve the operative field in endoscopic stapedectomy or not. Sixty patients aged from 18 to 50 years, undergoing endoscopic stapedectomy, were included in this prospective, randomized, double blinded, placebo-controlled study. Patients were randomly assigned to receive either metoprolol 100mg ( group 1) or a placebo, vitamin tablet, (group 2) 60min before surgery. The age, gender, body weight and ASA physical status were comparable between the two groups. The average blood loss in the placebo group was significantly greater than in the metoprolol group, but the time of operation in the metoprolol group was not significantly shorter. The surgical field was assessed using the Fromme-Boezaart scale, and those who received metoprolol had considerably cleaner (p0.001) surgical fields. The mean arterial pressure (MAP) didn't differ statistically significant at basal measurement up to 30min after anesthesia induction while it was significantly lower in the metoprolol group after 30min of induction up to end of surgery (p < 0.001). The heart rate was also significantly lower (p < 0.001) in patients who received metoprolol from prior to anesthesia induction up to end of surgery. metoprolol significantly improves visual clarity and hemodynamics during endoscopic stapedectomy.

Psychosocial Correlates of Emotional Eating Among Healthcare Professionals in a Tertiary Care Centre in Mysuru

Abstract Introduction: People’s food preferences and eating habits are heavily influenced by their psychological health. Emotional eating (EE) is not a distinct eating disorder, but rather a subtype within a range of different eating behaviors that are affected by habits, stress, emotions, and personal eating attitudes. Stress and eating are frequently unhealthy companions for those in the medical field. This study proposes to determine the prevalence and psychosocial correlates with EE among health-care professionals. Materials and Methods: A cross-sectional study was done among 320 health-care professionals at JSS Hospital, Mysore, over 6 months using a convenient sampling technique. A pretested, semistructured self-administered questionnaire was used to collect information regarding sociodemographic characteristics, perceived stress (Perceived Stress Scale), EE (Emotional Eaters Questionnaire), and eating and lifestyle habits of participants. Statistical tests such as Chi-square tests and Spearman’s correlation test were used. Results: Among 320 health-care professionals, 82.8% were emotional eaters. Among emotional eaters, 28.8%, 42.5%, and 11.5% were low, emotional, and very emotional eaters, respectively. Among the study subjects, 77.19% had moderate stress and 13.12% had severe stress. There was a statistically significant association between age, perceived stress, and fried food intake with EE. Conclusion: The findings raise concerns over the high prevalence of EE among health-care professionals, calling for close attention from policymakers to address this new health challenge. Enhancing dietary habits, body weight status, exercise, and perceived stress levels could be potential targets for interventions.

Interplay of Body Weight Status and Ethnicity With Sensory Perceptions of Food and Eating Behaviours

Interplay of Body Weight Status and Ethnicity With Sensory Perceptions of Food and Eating Behaviours

Modeling approaches for assessing device-based measures of energy expenditure in school-based studies of body weight status

BackgroundObesity has become an important threat to children’s health, with physical and psychological impacts that extend into adulthood. Limited physical activity and sedentary behavior are associated with increased obesity risk. Because children spend approximately 6 h each day in school, researchers increasingly study how obesity is influenced by school-day physical activity and energy expenditure (EE) patterns among school-aged children by using wearable devices that collect data at frequent intervals and generate complex, high-dimensional data. Although clinicians typically define obesity in children as having an age-and sex-adjusted body mass index (BMI) value in the high percentiles, the relationships between school-based physical activity interventions and BMI are analyzed using traditional linear regression models, which are designed to assess the effects of interventions among children with average BMI, limiting insight regarding the effects of interventions among children categorized as overweight or obese.MethodsWe investigate the association between wearable device–based EE measures and age-and sex-adjusted BMI values in data from a cluster-randomized, school-based study. We express and analyze EE levels as both a scalar-valued variable and as a continuous, high-dimensional, functional predictor variable. We investigate the relationship between school-day EE (SDEE) and BMI using four models: a linear mixed-effects model (LMEM), a quantile mixed-effects model (QMEM), a functional mixed-effects model (FMEM), and a functional quantile mixed-effects model (FQMEM). The LMEM and QMEM include SDEE as a summary measure, whereas the FMEM and FQMEM allow for the modeling of SDEE as a high-dimensional covariate. The FMEM and FQMEM allow the influence of the time of day at which physical activity is performed to be assessed, which is not possible using the LMEM or the QMEM. The FMEM assesses how frequently collected SDEE data influences mean BMI, whereas the FQMEM assesses the effects on quantile levels of BMI.ResultsThe LMEM and QMEM detected a statistically significant effect of overall mean SDEE on log (BMI) (the natural logarithm of BMI) after adjusting for intervention, age, race, and sex. The FMEM and FQMEM provided evidence for statistically significant associations between SDEE and log (BMI) for only a short time interval. Being a boy or being assigned a stand-biased desk is associated with a lower log (BMI) than being a girl or being assigned a traditional desk. Across our models, age was not a statistically significant covariate, and white students had significantly lower log (BMI) than non-white students in quantile models, but this significant effect was observed for only the 10th and 50th quantile levels of BMI. The functional regression models allow for additional interpretations of the influence of EE patterns on age-and sex-adjusted BMI, whereas the quantile regression models enable the influence of EE patterns to be assessed across the entire BMI distribution.ConclusionThe FQMEM is recommended when interest lies in assessing how device-monitored SDEE patterns affect children of all body types, as this model is robust and able to assess intervention effects across the full BMI distribution. However, the sample size must be sufficiently large to adequately power determinations of covariate effects across the entire BMI distribution, including the tails.

BMI, weight change, appetite reduction and cognitive impairment of elderly patients with diabetes

Body weight is related to both diabetes and cognitive impairment; however, the associations between body mass index (BMI) and cognitive impairment have been reported less frequently among diabetes patients. A total of 1355 patients with type 2 diabetes aged ≥ 60 years were included in this study. The Montreal Cognitive Assessment (MoCA) was administered to assess participants’ cognitive status. We collected self-reported body weight, weight loss and appetite loss data using questionnaires. Associations between body weight status (in childhood, midlife age, and late life), weight loss, appetite changes and cognitive impairment were explored using logistic regression. Among the participants, 41.7% exhibited cognitive impairment. Overweight in childhood and late life was associated with cognitive impairment among diabetes patients (OR 2.63, 95% CI 1.52–4.55; OR 1.32, 95% CI 1.03–1.69). Diabetes patients with cognitive impairment were more likely to report a body weight decline and appetite reduction in the past three months (OR 4.18, 95% CI 2.61–6.71; OR 4.41, 95% CI 2.67–7.29). Higher BMI, weight loss, and appetite reduction were positively correlated with cognitive impairment. Given the risk of cognitive impairment, we suggest that body weight and BMI decline should be monitored in patients with diabetes.

Population pharmacokinetics of mycophenolate in patients treated for interstitial lung disease (EVER-ILD study).

Mycophenolate mofetil (MMF) has been used to treat interstitial lung disease (ILD), but mycophenolate (MPA) pharmacokinetics was not reported for this use. This ancillary study of the EVER-ILD protocol aimed at describing the pharmacokinetic variability of MPA using population modelling in ILD. Concentrations of MPA were measured during an 8-h course for 27 ILD patients treated with 1000 mg MMF b.i.d. Absorption, distribution and elimination of MPA were described using population compartment models with first-order transfer and elimination rate constants, while accounting for both absorption peaks using gamma absorption models. The pharmacokinetics of MPA was best described using a two-compartment model and two gamma absorption models, model performances of this model were still similar to those of a one gamma absorption model. This pharmacokinetics seemed to be notably influenced by body weight, renal function and inflammatory status. The distribubtion value area under the concentration curve between two administrations of MMF was AUC12 = 52.5mg.h/L in median (interquartile range: 42.2-58.0mg.h/L). This is the first study reporting MPA pharmacokinetics in ILD. This pharmacokinetics appears to be similar to other indications and should be further investigated in future studies.

Neuroprotective effect of marrubiin against MPTP-induced experimental Parkinson’s disease in male wistar rats

In this work, we have analyzed the neuroprotective activity of marrubiin against MPTP-induced Parkinson’s disease (PD) in rat brains. MPTP (1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine) a neurotoxin was administered intraperitoneally (i.p.,) to rats and then treated using marrubiin. After marrubiin treatment, rats were trained, and tested for behavioral analyses like cognitive performance, open field test, rotarod test, grip strength test, beam walking test, the status of body weight, and striatal levels of neurotransmitters like dopamine, norepinephrine, serotonin, DOPAC, homovanillic acid, 5-hydroxy indole acetic acid, the status of oxidative stress markers like LPO, protein carbonyl content (PCC), Xanthine oxidase (XO), and status of antioxidant enzyme levels like SOD, CAT, GPX in the striatum and hippocampal tissues, status of neuroinflammatory markers like TNF-α, IL1β, IL-6, and status of histological architecture in brain striatum were also analyzed. All these parameters were significantly (p < 0.05) abnormal in MPTP-induced rats. Marrubiin (MB) treated shows significant (p < 0.05) near normal behavioral restoration in cognitive performance, open field, rotarod, grip strength, and beam walking tests. Furthermore, the status of body weight, and levels of neurotransmitters, were also significantly (p < 0.05) reversed to near normalcy in marrubiin-treated rats. Similarly, oxidative stress, antioxidant enzyme levels in the striatum and hippocampal tissues, TNF-α, IL1β, IL-6 levels, and histological architecture were noted to be restored to near normalcy in marrubiin-treated rats. Collectively, our preliminary results highlight the neuroprotective ability of marrubiin. However, the cellular and biochemical mechanisms of marrubiin’s neuroprotective ability have to be studied in detail.

Criterion-Related Validity of the Sugar-Sweetened Beverage (SSB) Questionnaire Among Children and Adolescents in Malaysia.

Sugar-sweetened beverages (SSBs) are a major source of dietary sugar, and their consumption is on the rise among children and adolescents. Excessive sugar intake is a significant contributor to overweight, obesity, and non-communicable diseases (NCDs). The consumption of SSBs, particularly that of children and adolescents, has been of interest as of late, as they are implicated in affecting body weight status. Thus, the goal of this study was to determine the predictive criterion-related validity of the SSB questionnaire that was administered to children and adolescents to assess their SSB and non-SSB intake. A nationwide cross-sectional study involving 5211 respondents aged 7-17 years old and their parents was conducted. The self-administered Malay questionnaire was distributed to collect information on socioeconomic background, the frequency of eating out at restaurants or other food premises, the availability of SSBs at home, and SSB consumption patterns of children and adolescents within a week. The predictive criterion-related validity was determined by using six hypotheses that can differentiate between two independent sample means of SSB consumption based on age, gender, locality, monthly household income, frequency of eating out at restaurants or other food premises, and availability of SSBs at home. The independent samples t-test and one-way ANOVA were used to conduct the validation process. Five out of six hypotheses were accepted. Significant mean differences were observed between sociodemographic factors, such as age (t=-10.56, p<0.001), localities (t=-5.37, p<0.001), monthly household income (F=26.83, p<0.001), and SSB consumption. Behavioural factors, including eating out at restaurants or other food premises (t=9.93, p<0.001) and environmental factors such as the availability of SSBs at home (F=136.24, p<0.001) also showed a significant difference with SSB consumption. The SSB questionnaire demonstrated the ability to differentiate between groups. Thus, this SSB questionnaire appears to be valid to measure the SSB consumption of children and adolescents.

RELATIONSHIP BETWEEN HOUSEHOLD ACTIVITIES PERFORMANCE AND BODY MASS INDEX OF HOUSEWIVES

Objective: The objective of this study was to find out the relationship between household activities performance and body mass index (BMI) of housewives. Methods: This cross-sectional study was undertaken among housewives residing in Shivalli village in Udupi district. The ages of the participants were between 30 and 45 years. The total number of the participants was 61. Household activities performance questionnaire was developed for data collection. The content of the questionnaire was validated by five registered occupational therapists with more than 15 years’ experience. The BMI of the participants was taken to ascertain their body weight status. Semi-structured interview was conducted to inquire about their household tasks using the developed household activities performance questionnaire. A diet chart with a total number of 7 days of meal was collected to calculate the participants’ calorie intake. The corresponding metabolic equivalent of task values for each mentioned task was assigned from compendium of physical activities (PA) to determine the relationship between household activities (manual and mechanical) performance and BMI of the participants. Results: While there was no significant negative correlation between housewives’ household manual activities performance and their BMI, housewives’ household mechanical activities performance and BMI showed a significant positive correlation. Sociodemographic characteristics showed no significant weak negative relationship with BMI score except socio-economic status which showed a significant weak positive relationship. Conclusion: Household activities performed by housewives might not have an impact on their BMI. However, combining household activities with moderate PA might tends to have an impact on the BMI of housewives. Further study with more sample size and a standardized questionnaire is recommended for future study.

Internet of things-based digital scale to detect stunting symptoms in babies under two years of age

Given the ongoing global challenge of stunting, characterized primarily by chronic underweight in infants under two years of age, a new approach leveraging digital scale and the internet of things (IoT) has been developed. This innovative system was designed to facilitate the early detection and continual monitoring of stunting symptoms caused by malnourishment. Key features include an IoT-enabled digital scale for precise weight measurement, a robust cloud platform for reliable data storage and comprehensive analysis, and an easy-to-use mobile app for user engagement. This system demonstrates its potential to simplify tracking fluctuations in baby weight and development progress related to stunting over time. Early trials demonstrated an impressive accuracy rate of 99.4% in body weight measurements and provided excellent conclusions in determining the body weight status of the infants. Overall, this IoT-based solution catalyzes the improvement of stunting detection methodologies and early intervention strategies, thus promising a better solution and a significant positive impact on global child health.

The Association between the Dietary Antioxidant Index and Weight Status in Primary School Students: An Epidemiological Study.

Obesity is an emerging threat and a current challenge for children and adolescents worldwide. The aim of the present work was to evaluate the relationship between the Dietary Antioxidant Index (DAI) and the weight status of students in early adolescence. A sample of 1580 students aged 10-12 years from 47 primary schools in Greece were enrolled. Anthropometric characteristics were assessed, and calculation of the Body Mass Index (BMI) was used to categorize students into two weight-status groups. Dietary habits and physical activity were evaluated using a self-completed questionnaire, and the DAI was calculated through derived micronutrients' content, along with energy, macro-, and micro-nutrient intake. Crude and adjusted regression analysis showed a significant inverse association of the DAI and body weight status (Odds Ratio (OR): 0.719, 95% Confidence Interval (CI): 0.576; 0.897, and adjusted Odds Ration (aOR): 0.667, 95% CI: 0.489; 0.907). An antioxidant diet seems to play a protective role against increased body weight among students in early adolescence. Thus, dietary patterns rich in antioxidants should be promoted to facilitate healthy habits early in life, and to fight the obesity threat.

Population Pharmacokinetics of Bepirovirsen in Healthy Participants and Participants with Chronic Hepatitis B Virus Infection: Results from Phase 1, 2a, and 2b Studies.

Bepirovirsen is a novel antisense oligonucleotide in development for chronic hepatitis B virus (HBV) infection therapy. Understanding the impact that clinical characteristics may have on bepirovirsen exposure is important for determining efficacious and well-tolerated dosing regimens. This analysis evaluated demographics and clinical characteristics associated with bepirovirsen exposure using a population pharmacokinetic (PK) analysis. Population PK analyses were conducted using pooled data from three phase 1/2 clinical studies (NCT03020745/NCT02981602/NCT04449029) to construct a structural PK model for bepirovirsen that adequately described plasma concentration-time profiles and identify covariates that affect systemic exposure. The final population PK model was used to simulate bepirovirsen exposure measures to inform exposures at different dose levels and within different subpopulations. Bepirovirsen PK data were well-described by a linear, three-compartment model with first-order absorption and absorption delay. Chronic HBV infection status, body weight, and Asian versus non-Asian race were key covariates included in the final model. Visual inspection of correlation scatter plots confirmed general agreement between observed and predicted data from the studies. In simulations, bepirovirsen systemic exposure was dosed proportionally and predicted to be almost completely washed out by 12weeks following the final 300-mg dose. Differences in body weight, Asian race, or disease status did not result in clinically relevant differences in exposure. This analysis demonstrated that the linear three-compartmental model accurately described bepirovirsen PK data. The lack of clinically relevant differences seen in exposure indicate that dose adjustments are not recommended for bepirovirsen based on demographics or clinical characteristics.

Perception of Body Weight and Nutritional Status in Adolescents in Northeastern Mexico

Objetivo: Evaluar la percepción del peso corporal en adolescentes e identificar predictores del estado nutricional. Método: Diseño descriptivo, correlacional. La población estuvo conformada por estudiantes de una institución educativa pública seleccionada de forma aleatoria, ubicada en el noreste de México. La muestra fue de 851 adolescentes que cursaban entre el 7º y 9º grado de educación básica inscritos en la institución educativa seleccionada. Se evalúo la satisfacción con el peso corporal mediante las figuras de Stunkard y Sørensen. Se realizaron mediciones antropométricas (peso y talla) y se calculó el IMC. Resultados: El 18.8 % (n= 160) y 19.1% (n= 163) de los participantes presentaron sobrepeso y obesidad, respectivamente; el 21.6 % (n= 185) pertenecen al sexo masculino y 16.3 % (n= 138) al femenino. Por otro lado, el 67.5 % (n= 574) de los participantes reportó insatisfacción corporal, es decir el 45.2 % (n= 384) desea estar más delegado(a), lo cual es más frecuente en participantes del sexo femenino. La insatisfacción con el peso corporal (?= -2.62, p= .001), edad (?= .29, p= .020) y sexo (?= -.642, p= .005) influyen en el IMC de los adolescentes Conclusiones: La edad, sexo y la percepción del peso corporal son predictores del índice de masa corporal en los adolescentes del estudio.

Impact of a digital employer-based weight loss program on individuals age 65 or older.

Older adults are not protected from obesity, which has been linked to frailty, cognitive impairment, and other aging-related factors. Intensive lifestyle interventions have been shown to be effective for weight loss in older adults; however, these have typically been highly intensive and less feasible for dissemination. This analysis describes weight loss in a large-scale, commercially available, digital intervention in a subset of older adults. Older adults (N = 20,443, males = 6,238; females = 14,205) between 65 and 85years of age with overweight (43.3%) or obesity (46.7%) participated in an online, self-directed weight loss program. Behavioral-based content was delivered through weekly video lessons within an online platform that included weight and physical activity tracking, an online community, a reference library, and access to coaching support. Self-reported measures taken at the time of entry into the program were used for this analysis (demographics, height, body weight, and health status). Weight was reported across weeks of engagement in the curriculum. The average weight loss was -3.15kg (95% CI: [-3.20, -3.11]) at 15.5weeks. Weight loss was significantly greater in male individuals (-3.79kg [95% CI: -3.89, -3.71]) versus female individuals (-2.87kg [95% CI: -2.94, -2.82]) (p < 0.001), with a similar engagement in curriculum weeks. Percent weight loss was statistically significant for all age categories (p < 0.05) and self-reported health conditions (p < 0.05). Short-term weight loss was observed in older adults exposed to a low-touch, self-guided, and digital behavioral-based weight loss program. Weight loss was also observed even in the presence of various chronic health conditions.

A systematic review on the applicability of cell-free DNA level as an obesity biomarker.

Obesity has become a global health concern in recent decades. Utilizing biomarkers presents a promising approach to comprehensively monitor the progress of obesity and its associated health conditions. This review aims to synthesize the available evidence on the correlation between cfDNA level and obesity and to provide insights into the applicability of using cfDNA level as a tool for monitoring progression of obesity. Searches were performed in PubMed and Embase on April 1, 2022. Data and other relevant information were extracted and compiled into a structured table for further analysis. Among 1170 articles screened, 11 articles were included in this review and assessed qualitatively. The results demonstrated that existing evidence mainly focused on three populations, including healthy individuals, cancer patients and pregnant women. Majority of the studies on healthy individuals identified a significant association between cfDNA level and body weight status but not among cancer patients. Varying results were observed among pregnant women at different gestational trimesters. Our review summarized some preliminary evidence on the association between cfDNA level and obesity. More cohort studies in larger scale with comprehensive assessment have to be conducted to examine the applicability of cfDNA as a biomarker for severity and disease progression of obesity.

Effects of Breaking Up Sitting on Gut Hormone Responses and Subsequent Compensatory Behaviors in Physically Inactive Adults.

The effects of breaking up sitting on gut hormone responses and free-living energy compensatory behaviors are still unclear in people of Asian ethnicity. Twenty-six Asians including 13 lean individuals (Lean) and 13 individuals with centrally overweight/obesity (OW), aged between 20 and 45 yr, completed a randomized crossover study with either 5.5-h uninterrupted sitting (SIT) or 5.5-h sitting with 2-min walking at 6.4 km·h -1 every 20 min (ACTIVE) in the laboratory. Blood samples were collected at regular time points to examine postprandial glucagon-like peptide 1 (GLP-1), peptide YY (PYY), and glucose-dependent insulinotropic polypeptide (GIP) concentrations. Free-living physical activity and energy intake were recorded using wearable devices and weighed food diaries outside the laboratory until midnight. Paired t -tests were conducted to compare responses between trials. Postprandial GLP-1 and PYY incremental area under the curve values were higher in the ACTIVE trial versus SIT in both Lean and OW groups (all, P < 0.05), but there was no difference in GIP in either group (both, P > 0.05). There were no differences in free-living physical activity (volume and intensity) or energy intake (total and macronutrients) between trials in either group (all, P > 0.05), resulting in greater total physical activity over the 24-h monitoring period in ACTIVE trial versus SIT trial (both, P < 0.05). Breaking up sitting increases postprandial GLP-1 and PYY concentrations in Asians, but does not induce subsequent behavioral compensation, resulting in greater 24-h physical activity levels and lower relative energy intake, in inactive individuals irrespective of bodyweight status.

Effects of dietary seaweed on obesity-related metabolic status: a systematic review and meta-analysis of randomized controlled trials.

Seaweed is a promising source of anti-obesity agents, including polysaccharides, proteins, polyphenols, carotenoids, and n-3 long-chain polyunsaturated fatty acids. The anti-obesity effects of such compounds may be due to several mechanisms, including inhibition of lipid absorption and metabolism, effect on satiety, and inhibition of adipocyte differentiation. The aim of this study was to assess the evidence from human randomized controlled trials for the effects of seaweed on body-weight status as well as lipid and nonlipid parameters in adults with overweight and obesity. Four databases-Medline, Scopus, Web of Science, and Cochrane Library-were searched from December 2022 to June 2023 using the following key words: Seaweed OR fucoxanthin OR alginates OR fucoidans OR phlorotannin's OR macroalgae OR marine algae AND obesity OR overweight OR BMI OR body mass index. Eleven interventional studies (10 parallel and 1 crossover) were extracted. Meta-analysis showed a significant effect, favoring the intervention group for BMI (body mass index) (standardized mean difference [SMD]: -0.40; 95% CI: -0.65 to -0.16 kg/m2; P = 0.0013) and percentage of fat mass (SMD: -1.48; 95% CI: -2.66% to -0.30%, P = 0.0138). The results were seen when refined or extracted brown seaweed (BMI) or only refined brown seaweed (% fat mass) were administered to participants for at least 8 weeks. Moreover, a significant overall effect of seaweed supplementation on total cholesterol (SMD: -7.72; 95% CI: -12.49 to -2.95 mg/dL; P = 0.0015) and low-density-lipoprotein cholesterol (SMD: -7.33; 95% CI: -11.64 to -3.02 mg/dL; P < 0.001) was noted. Any significant effects of seaweed on glucose metabolism were not shown. Edible seaweed supplementation shows potential for managing obesity and disorders of the blood lipid profile when administered to participants for at least 8 weeks. PROSPERO registration no. CRD42022378484 (www.crd.york.ac.uk/PROSPERO).

Is problematic use of the Internet and smartphone predictor of unhealthy eating behaviors and abnormal body weight in Turkish young adults?

With technological progress, the use of the internet and smartphones has become an inseparable part of our lives and their use is increasing. The uncontrolled use of both the internet and smartphones is defined as problematic use. This study examined the effects of problematic internet and smartphone use on dietary behaviors and abnormal body weight status in young adults. Participants were 560 university students. Participants completed measures of demographics, dietary behaviors, internet and smartphone usage habits, Young's Internet Addiction Test and Smartphone Addiction Scale. Negative associations were found between dietary behaviors and problematic internet and smartphone use. Significant inverse associations were also found between these uses and recommended consumption levels of several food groups. For a healthy future generation, it is recommended that policies be developed to prevent or manage these problematic uses, especially in young adults with negative dietary behaviors and abnormal body weight.

PSIII-4 Evaluating the variability of the dual sugar absorption test as a measure of intestinal permeability in pigs

Abstract The intestinal barrier of pigs can be compromised by many factors including health challenges, off-feed events, and environmental stressors. If dysregulated barrier function is caught early, proper treatment can be initiated to potentially prevent more severe complications to overall health. However, there are few procedures to measure intestinal function in pigs that are both validated, sensitive, and minimally invasive. In this research, the dual sugar test using lactulose and mannitol was conducted on a total of 534 pigs of varying age, sex, body weight (BW), and health status. Each pig received the same oral dose of 500 mg/kg body weight lactulose and 50 mg/kg BW mannitol, and blood was collected either 60 or 240 min following the dose. Plasma concentrations of lactulose and mannitol were quantified by high performance liquid chromatography using validated methods. The ratio of lactulose to mannitol absorption (L:M) served as the indicator of barrier function such that a larger L:M ratio was indicative of greater intestinal permeability. In these pigs, L:M ratios ranged from 0 to 6.96. The median L:M ratio was 0.41 while the average was 0.70, indicating that the distribution of the data was right skewed, influenced by 21.0% of pigs exhibiting a L:M of 0. Collecting blood sooner after oral gavage yielded better detectability as only 2.71% of L:M in pigs from the 60 min group was 0 compared with 43.5% from the 240 min group. Ostensibly, an L:M ratio of 0 is biologically relevant, but interpretation of such values is challenging both from statistical and biological perspectives. Intestinal barrier permeability, absorption kinetics between pigs, and delivery efficiency of the L:M solution may all affect lactulose absorption yielding a ratio of 0. In general, the data werewere highly variable, with a standard deviation of 0.95, so a regression analysis was performed to determine which factors may be influencing this variation. Neither a forward nor a backward elimination model determined any of the five explanatory variables (i.e., BW, timing of blood collection, health status, age, or sex) to be significant. Additional correlational analyses further confirmed that there were no significant interactions. Overall, it appears that these factors do not help to explain the variability in the L:M ratio. Further investigation is warranted to validate this process and understand the source and impact of L:M ratios equaling 0.

Allostatic load, adverse childhood experiences, executive functions, and BMI status in adolescents and young adults.

Chronic stress induces preclinical changes in the metabolic, cardiovascular, and immune systems. This phenomenon, known as allostatic load (AL), can impair executive functions (EF), which may be even more affected in individuals with excess weight due to their characteristic inflammatory state and cardiometabolic changes. Adverse childhood experiences (ACEs) contribute to AL and may influence executive functioning presumably via alterations within the hypothalamic-pituitary axis, including epigenetic modifications. We assess the relationship between AL and EF in youth with and without excess weight, and the effect ACEs on executive functioning. One hundred eighty-two adolescents and young adults (85 with normal weight and 97 with overweight/obesity; 10-21 years) were recruited. The estimated AL index included the following: systolic and diastolic blood pressure, glycated hemoglobin, high- and low-density lipoprotein cholesterol, triglycerides, high-sensitivity C-reactive protein, fibrinogen, and cortisol. ACEs were measured using the Juvenile Victimization Questionnaire. The neuropsychological evaluation included the assessment of inhibition, working memory, and cognitive flexibility processes. AL was not significantly associated with executive functioning, and this relationship did not depend on body-weight status. ACEs, available for 57 of 182 participants, were significantly associated with poorer executive functioning. Our study shows that AL is not associated with executive functioning in adolescents and young adults. Since the current sample was young, we hypothesize that a longer exposure to AL might be required for its negative effects to surface. Nevertheless, exposure to early adversity seems to be associated with poorer executive functioning in youth.