Blood Glucose Levels In Fish Research Articles

THE EFFECT OF TRANSPORTATION ON BLOOD GLYCOSE LEVELS AND ECTOPARASITE INVESTMENT IN PUNTEN CARP (Cyprinus carpio)

Carp is one of the most popular sources of animal protein. Aquaculture products must be distributed through a transportation system to meet the demand for carp. Improper transportation techniques will cause the condition of the fish to deteriorate, thereby increasing stress, the parasites susceptible enter the fish's body. The aim of this study was to determine the effect of transportation on blood glucose levels and ectoparasite infestation in Punten carp. The method used in this study is an experimental method with descriptive approach by comparing the values of blood glucose levels and parasite infestations in fish before the transportation process and after the transportation process. The results of the analysis of water quality before the transportation and after the transportation process still within normal limits. In the transportation treatment, Mas Punten fish can increase blood glucose levels significantly with a value before transportation of 31.54 mg/dL and after transportation of 51.41 mg/dL. The increase in ectoparasite survival was not significant with an average number of 10.64 ind/head (medium) before transportation and 12.08 ind/head (moderate) after the transportation process. There is a correlation between blood glucose levels and ectoparasite infestation with the degree of correlation. Based on the chi test, it was found that the treatment of the Punten Mas fish transportation system can affect the blood glucose level of fish very significantly (sig> 0.01), but it is not associated with an increase in ectoparasite infestations that occur (.338>0.01).

RESPONS GLUKOSA DARAH BENIH IKAN JELAWAT (Leptobarbus hoeveni Blkr) DALAM MEDIA YANG DIBERI EKSTRAK DAUN UBI JALAR (Ipomoea batatas)

Use of sweet potato leaf extract to determine the blood glucose response of jelawat fish (L. hoeveni, Blkr) seeds maintained in the media.This study aims to determine the optimal concentration of sweet potato leaf extract to reduce the stress level of Jelawat fish (L. hoeveni, Blkr) seeds by measuring blood glucose. In this study, four treatments were used with three replications using a concentration of 2ml / L sweet potato leaf extract, 4ml / L extract concentration, 6ml / L extract concentration, and control. The results showed that the 2ml / L extract concentration was the best because it was proven that the fish blood glucose response at every 1st, 6th, 12th hour, 24th hour, 168th hour fish (L. hoeveni, Blkr) seed blood glucose levels nettle nearly close to normal blood glucose levels in fish.

Wireless enzyme sensor system for real-time monitoring of blood glucose levels in fish

Periodic checks of fish health and the rapid detection of abnormalities are thus necessary at fish farms. Several studies indicate that blood glucose levels closely correlate to stress levels in fish and represent the state of respiratory or nutritional disturbance. We prepared a wireless enzyme sensor system to determine blood glucose levels in fish. It can be rapidly and conveniently monitored using the newly developed needle-type enzyme sensor, consisting of a Pt–Ir wire, Ag/AgCl paste, and glucose oxidase. To prevent the effects of interfering anionic species, such as uric acid and ascorbic acid, on the sensor response, the Pt–Ir electrode was coated with Nafion, and then glucose oxidase was immobilized on the coated electrode. The calibration curve of the glucose concentration was linear, from 0.18 to 144 mg/dl, and the detection limit was 0.18 mg/dl. The sensor was used to wirelessly monitor fish glucose levels. The sensor-calibrated glucose levels and actual blood glucose levels were in excellent agreement. The fluid of the inner sclera of the fish eyeball (EISF) was a suitable site for sensor implantation to obtain glucose sample. There was a close correlation between glucose concentrations in the EISF and those in the blood. Glucose concentrations in fish blood could be monitored in free-swimming fish in an aquarium for 3 days.

Effects of Graded Levels of Carbohydrate on Growth and Survival of Largemouth Bass, Micropterus salmoides

Abstract Excessive carbohydrates (CHO) in diets for largemouth bass (LMB), Micropterus salmoides, are suspected of accumulating glycogen in hepatocytes, which may result in liver dysfunction. This study evaluated the effect of graded levels of dietary CHO on growth, survival, and liver histology of LMB. One hundred feed‐trained advanced fingerling LMB (128.5 ± 21.5 g) were stocked into each of nine 3400‐L polyethylene tanks. Tanks were randomly assigned one of three experimental diets containing different CHO levels (13, 19, or 25% of diet). The extruded diets were approximately isonitrogenous (42% crude protein) and isocaloric (3 kcal/g energy). There were three replicate tanks per dietary treatment. Bass were fed to apparent satiation twice daily for 148 d. Survival was significantly higher (P ≤ 0.05) for fish fed the 13 and 19% CHO diets (89 and 90%, respectively) compared to those fed the 25% CHO diets (82%). Average harvest weight of fish fed the 13% CHO diet (380 g) was significantly greater (P ≤ 0.05) than for fish fed other diets. Average harvest weight of fish fed the 19% CHO diet (347 g) was significantly greater (P ≤ 0.05) than for fish fed the 25% CHO diet (310 g). Specific growth rates (%/d) were significantly higher (P ≤ 0.05) in fish fed the 13 and 19% CHO diets than in fish fed 25% CHO diet. Feed conversion ratios for fish fed the 13 and 19% CHO diets (2.3 and 2.4, respectively) were both significantly lower (P ≤ 0.05) than in fish fed the 25% CHO diet (3.6). There were no significant differences (P > 0.05) in condition factor, protein efficiency ratio, hepatosomatic index, or liver glycogen concentration among fish fed the different experimental diets. Overall, mean blood glucose levels in fish fed the 13 and 19% CHO diets (61.0 and 71.2 mg/dL, respectively) were significantly lower (P ≤ 0.05) than in fish fed the 25% CHO diet (87 mg/dL). Histopathological examination of livers from fish fed the three diets was used to score the degree of vacuolization of hepatic tissues (0 = normal, 1 = slight, 2 = mild, 3 = moderate, and 4 = severe). Regression of vacuolization scores on dietary CHO levels was statistically significant (P ≤ 0.05) and indicated a direct positive relationship between liver vacuolization and dietary CHO level (R2 = 0.57). These data indicate that LMB grow faster and use feeds more efficiently when CHO are maintained at <20% of diet. CHO levels >20% negatively impacted liver histology, but a liver tissue analyses did not document glycogen accumulation.

Different capabilities in utilizing dietary carbohydrate by fingerling and subadult giant gouramy Osphronemus gouramy

: Two experiments were conducted to determine the capability of the giant gouramy Osphronemus gouramy to assimilate dietary carbohydrate. In experiment 1, fingerlings with an initial body weight of 29–32 g were fed diets containing 20.8, 35.6, 49.8 and 57.0% carbohydrate three times daily, to satiation, for 40 days. In experiment 2, subadults with an initial body weight of 78.7–79.5 g were fed diets containing 21.2, 30.1, 38.6 and 47.5% carbohydrate three times daily, to satiation, for 60 days. The diets had the same amount of protein and the same energy content. The results of experiment 1 showed that the blood glucose levels of fish fed high carbohydrate diets (49.8 and 57.0%) in the 18 h postprandial were lower than those of fish fed low carbohydrate diets (20.8 and 35.6%). The peak of the blood glucose levels in all treatments was found 5 h postprandial. Experiment 2 showed that the blood glucose level of fish was the same among treatments; and the peak of the blood glucose levels was found 9 h postprandially. It was also found that the protein retention, daily growth rate and feed efficiency of the dietary 20.8% carbohydrate levels in experiment 1 were all significantly higher than in other treatments; whereas those in experiment 2 showed no significant difference among the treatments (P > 0.05). In both experiments 1 and 2, lipid retention increased significantly as the dietary carbohydrate level was elevated (P < 0.05). It was concluded that fingerlings of giant gouramy have a lower capability for utilizing dietary carbohydrate, and of the treatments, the 20.8% diet was optimal, whereas subadults could utilize diets with higher carbohydrate levels, as high as 47.5%.

Influence of inorganic lead on the biochemical blood composition in the rainbow trout, Salmo gairdneri

Rainbow trout ( Salmo gairdneri), kept in slightly hypotonic brackish water, were exposed to inorganic lead in three different concentrations (10, 75, and 300 μg Pb/liter) for 30 days and then allowed to recover in lead-free brackish water for 49 days. Analyses of residue levels after exposure revealed a rapid dose-dependent accumulation of lead in the liver, while this process was slower in muscle tissue. After recovery, the lead levels dropped markedly in the liver, but were only changed to a minor degree in the muscle. Analyses of the biochemical blood composition showed that lead caused disturbances in the ion regulation, indicated by dose-dependent increases in blood plasma potassium concentrations after both exposure and recovery, and by a dose-dependent reduction in blood plasma chloride concentrations after recovery. Furthermore, an impaired glucose homeostasis was suggested by a significant reduction in blood glucose levels in fish after exposure to inorganic lead.

Effects of endosulfan on fish carbohydrate metabolism

Exposure of the Indian catfish ( Heteropneustes fossilis) to a high sublethal concentration of 0.0015 ppm (0.75 of the 96-hr LC 50) of endosulfan for 3, 6, 12, 48, and 96 hr affected carbohydrate metabolism. Muscle glycogen levels decreased significantly at 3, 6, 48, and 96 hr; liver glycogen content declined at 6 hr but there was a resynthesis of liver glycogen stores at 12 hr. Blood glucose levels in fish were elevated at 3, 6, 48, and 96 hr. Mean values of blood pyruvate were elevated significantly at 6 and 48 hr. Blood lactate levels were elevated at 3 and 6 hr but hypolacticemia resulted at 12 and 96 hr in pesticide-treated fish. The observed effects of endosulfan on carbohydrate metabolism in fish are discussed in relation to acute stress syndrome. Measurement of carbohydrate metabolites in fish for 3 hr or longer could prove useful as a rapid method for evaluating toxicity of pesticides and other toxicants.

Effect of a paired mixture of aldrin and formothion on carbohydrate metabolism in a fish, Heteropneustes fossilis

Exposure of the Indian catfish ( Heteropneustes fossilis) to a high sublethal concentration, 0.114 ppm (0.8 of the 96-hr LC 50) of a mixture of aldrin and formothion, added in a one-to-one ratio, for 3, 6, 12, 48, and 96 hr affected carbohydrate metabolism. Muscle glycogen decreased significantly at 6 and 48 hr; hepatic glycogen content declined at 3, 6, 12, and 48 hr in exposed fish. Blood glucose levels in fish were significantly decreased at 3, 12, 48, and 96 hr. Mean value of blood pyruvate was significantly depressed at 3 hr. Blood lactate levels were elevated at 3, 6, 12, and 96 hr in pesticide-treated fish. The observed effects of the mixture of aldrin and formothion on carbohydrate metabolism in fish could prove useful as a rapid method for evaluating toxicity of pesticides and other toxicants.

Effects of acute exposure to methyl parathion on carbohydrate metabolism of Indian catfish (Heteropneustes fossilis).

Exposure of the Indian catfish (Heteropneustes fossilis) to a high sublethal concentration of 5.6 p.p.m. (0.8 of the 96 hr LC50) of methyl parathion for 3, 6, 12, 48, and 96 hrs affected carbohydrate metabolism. Muscle glycogen levels decreased significantly at 3, 6, 12 and 96 hrs; liver glycogen content declined at 6 hrs but there was a resynthesis of hepatic glycogen stores at 12 hrs. Blood glucose levels in fish were elevated at 3 and 6 hrs. Mean values for blood pyruvate were elevated significantly at 6, 12, and 96 hrs. Blood lactate level was elevated at 3 hrs but hypolactaemia resulted at 48 and 96 hrs in pesticide-treated fish. The observed effects of methyl parathion on carbohydrate metabolism in fish are discussed in relation to acute stress syndrome. Measurement of carbohydrate metabolites in fish for 3 hrs or longer could prove useful as a rapid method for evaluating toxicity of pesticides and other toxicants.