Black-eared Opossum Didelphis Research Articles

A putative new Besnoitia species in the southern black-eared opossum Didelphis aurita

Besnoitia spp. are cyst-forming coccidian parasites with a broad host range, infecting various wild and domestic animal species. Northamerican opossums (Didelphis virginiana) are severely affected by the infection with B. darlingi. This study presents a case of infection with Besnoitia in a road-killed female southern black-eared opossum (Didelphis aurita) in Misiones, Argentina. Many 0.5 to 1 mm cysts were observed in several muscles and visceral organs and were microscopically identified in skeletal muscles, tongue, and heart. Histological analysis disclosed multiple spherical cysts with a myriad of bradyzoites like-cells and a well-defined cyst wall. A small number of degenerate and ruptured cysts, surrounded by mild to moderate inflammation were observed. Genomic DNA from an individual cyst and muscle was extracted and ITS1 marker and 18S rRNA gene fragments from sarcocystid protozoa were successfully amplified by PCR and sequenced. The 18S sequence exhibited 100% identity with sequences of B. darlingi and B. oryctofelisi. Comparison of the complete ITS1 sequence (259 bp) revealed an identity of 99.2% with B. oryctofelisi and 97.7% with B. darlingi. This result together with the phylogeny positioning, suggest that the Besnoitia sp. in the present case differ from B. darlingi, being closely related with B. oryctofelisi.

A longitudinal survey of gastrointestinal parasites of the black-eared opossum Didelphis aurita at an urban-sylvatic interface area in Southeast Brazil: a morphological and ecological characterization based on helminth eggs.

Parasites are important organisms in ecosystem dynamics and take part in the regulation and structure of host populations. The prevalence, abundance, and distribution of parasites in their host species may be affected by biotic and abiotic factors. Most studies of helminths of Brazilian marsupials are taxonomic descriptions or re-descriptions and records of occurrence. The use of noninvasive techniques for studying parasitic worms of vertebrate hosts is more common in large or threatened species. The aims of this study were to describe and identify the helminth morphotypes and to analyse the parasitological parameters of gastrointestinal helminth eggs obtained in faecal samples of marsupial Didelphis aurita at the Fiocruz Atlantic Forest Biological Station and surroundings, municipality of Rio de Janeiro, southeast Brazil. The common opossums were sampled during a capture-mark-recapture study. Faecal samples collected from each animal were analysed for helminth egg diagnosis using the methods of flotation in sugar solution and sedimentation. Eggs were compared with samples obtained from the uterus of adult worms obtained from a previous study carried out in the same area. Eleven helminth egg morphotypes were found. Among them, seven were identified at the species level. The highest values of parasitic load and prevalence were observed for the families Viannaiidae and Trichuridae. A significant relationship was found between the number of morphotypes and host body size in females. The influence of parasite load on host condition factor or body size was not observed.

A successful multiple litter adoption in a nursing southern black-eared opossum, Didelphis aurita (Didelphimorphia, Didelphidae)

Abstract Adoption by an alloparent of an offspring or a litter occurs in at least eight mammal orders, but is rare in marsupials, and was never confirmed in opossums. Here we report the first unequivocal and documented successful event of alloparental adoption of two distinct litters by a single captive female Southern Black-eared Opossum Didelphis aurita (Didelphimorphia, Didelphidae). The female, still nursing her biological litter, accepted and nursed the two offered litters. All three litters were raised simultaneously, and the female cared for and displayed protective behaviour indistinctively of the biological or adopted pups. She was kept captive until released along with her biological litter, which was weaned and fled independently, and the two adoptive litters clinging on her back. This event sheds additional light on the little known social and maternal behaviour of opossums, suggesting it is possibly more complex than originally thought.

Metacommunity structure of the helminths of the black-eared opossum Didelphis aurita in peri-urban, sylvatic and rural environments in south-eastern Brazil.

Among the Brazilian marsupials, the species of the genus Didelphis are the most parasitized by helminths. This study aimed to describe the species composition and to analyse the helminth communities of the Atlantic Forest common opossum Didelphis aurita at infracommunity and component community levels using the Elements of Metacommunity Structure Analysis, considering peri-urban, sylvatic and rural environments in the state of Rio de Janeiro, Brazil. Seventy-three specimens of D. aurita were captured during the study. Fourteen species of helminths were collected: nine of the phylum Nematoda (Trichuris minuta, Trichuris didelphis, Globocephalus marsupialis, Heterostrongylus heterostrongylus, Travassostrongylus orloffi, Viannaia hamata, Aspidodera raillieti, Cruzia tentaculata and Turgida turgida); four of the phylum Platyhelminthes (three species of Trematoda, Duboisiella proloba, Brachylaima advena and Rhopalias coronatus, and one species of the class Cestoda); and one species of the phylum Acanthocephala (Oligacanthorhynchus microcephalus). More than 95% of the animals were infected with parasites. The most abundant parasite species were V. hamata, T. turgida, C. tentaculata and T. orloffi. The nematodes T. turgida, C. tentaculata and A. raillieti were the most dominant species in the three environments. The analysis of the metacommunity structure indicated a structured pattern as a function of the environmental gradient, but only when all localities were considered together. At the infracommunity and component community levels, quasi-nested and nested structures with stochastic species loss were observed, respectively. The results indicate that the settlement of helminth species in the opossums can be attributed to the heterogeneity among individual hosts in relation to their exposure to parasites and to extrinsic factors, which vary among habitats, considering a regional scale. On a local scale, the characteristics of this host species may promote homogenization among individual hosts in terms of their exposure to the helminths. This study constitutes a new host record for the species G. marsupialis and T. didelphis and represents a pioneering investigation of the helminth metacommunity of a Neotropical marsupial.

Resource-defense behaviour: first report of an agonistic interaction between the opossumDidelphis auritaand the batArtibeus lituratus

Species coexistence depends mainly on the spatiotemporal distribution of resources, and, in extreme cases, results in direct competition (interference). In the present study, we report for the first time an agonistic interaction between marsupials and bats, and food defense behaviour in the black-eared opossum Didelphis aurita. We describe the agonistic interaction between the opossum and a bat of the species Artibeus lituratus while they foraged on the same infructescences of Cecropia glaziovii and C. hololeuca in an Atlantic Forest area. While competing for food, the bats foraged in group (of approximately six individuals) and apparently showed a synchronized and coordinated behaviour to pick the infructescences. Then, a male D. aurita showed an aggressive behaviour toward the bats, moving on branches and leaf petioles and vocalizing to the bats, apparently trying to scare them away. We conclude that D. aurita defends food in a way consistent with the concept of economic defendability. We know little about how Neotropical mammals share resources in time and space, so future studies on the foraging behaviour of the black-eared opossum should focus on agonistic interaction with competitive species.

Prey Preferences of the Jaguar Panthera onca Reflect the Post-Pleistocene Demise of Large Prey

Documenting the impacts of the Pleistocene megafaunal extinctions on predator-prey interactions is a challenge because of the incomplete fossil record and depauperate extant community structure. We used a comparative ecological approach to investigate whether the existing prey preference patterns of jaguars Panthera onca were potentially affected by the Pleistocene extinctions in the Americas compared with large felids in Africa and Asia. We reviewed the literature and found 25 studies reporting 3214 jaguar kills recorded throughout the species’ distribution. We found that jaguars significantly preferred capybara Hydrochaeris hydrochaeris and giant anteater Myrmecophaga tridactyla, and avoided agoutis, carnivorans, primates, black-eared opossum Didelphis marsupialis and tapirs. Generalised linear models showed that jaguars select prey primarily based on socio-ecological and behavioural traits (abundance and herd size), rather than morphological characteristics (body size). Nonetheless, their accessible prey weight range was 6-60 kg, preferred prey weight range was 45-85 kg, and mean mass of significantly preferred prey was 32 ± 13 kg leading to a predator to prey body mass ratio of 1:0.53, which is much less than that of other solitary felids. Compared with other large, solitary felids, jaguars have an unusual predator to prey body mass ratio, show limited effect of prey morphology as a driver of prey selection, lack evidence of optimal foraging beyond their preferred prey, and an absence of preferentially hunting on Cetartiodactyla herbivores. These features, coupled with the reduction in jaguar body mass since the Pleistocene, suggest that the loss of larger potential prey items within the preferred and accessible weight ranges at the end-Pleistocene still affects jaguar predatory behaviour. It may be that jaguars survived this mass extinction event by preferentially preying on relatively small species.

Edge effects and geometric constraints: a landscape-level empirical test.

Edge effects are pervasive in landscapes yet their causal mechanisms are still poorly understood. Traditionally, edge effects have been attributed to differences in habitat quality along the edge-interior gradient of habitat patches, under the assumption that no edge effects would occur if habitat quality was uniform. This assumption was questioned recently after the recognition that geometric constraints tend to reduce population abundances near the edges of habitat patches, the so-called geometric edge effect (GEE). Here, we present the first empirical, landscape-level evaluation of the importance of the GEE in shaping abundance patterns in fragmented landscapes. Using a data set on the distribution of small mammals across 18 forest fragments, we assessed whether the incorporation of the GEE into the analysis changes the interpretation of edge effects and the degree to which predictions based on the GEE match observed responses. Quantitative predictions were generated for each fragment using simulations that took into account home range, density and matrix use for each species. The incorporation of the GEE into the analysis changed substantially the interpretation of overall observed edge responses at the landscape scale. Observed abundances alone would lead to the conclusion that the small mammals as a group have no consistent preference for forest edges or interiors and that the black-eared opossum Didelphis aurita (a numerically dominant species in the community) has on average a preference for forest interiors. In contrast, incorporation of the GEE suggested that the small mammal community as a whole has a preference for forest edges, whereas D.aurita has no preference for forest edges or interiors. Unexplained variance in edge responses was reduced by the incorporation of GEE, but remained large, varying greatly on a fragment-by-fragment basis. This study demonstrates how to model and incorporate the GEE in analyses of edge effects and that this incorporation is necessary to properly interpret edge effects in landscapes. It also suggests that geometric constraints alone are unlikely to explain the variability in edge responses of a same species among different areas, highlighting the need to incorporate other ecological factors into explanatory models of edge effects.

Frugivory by the black-eared opossum Didelphis aurita in the Atlantic Forest of southern Brazil: Roles of sex, season and sympatric species

Our objective in this study was to examine the frugivory performed by the black-eared opossum, Didelphis aurita Wied-Neuwied, 1826, in an area of the coastal Atlantic Forest of southern Brazil, including differences between sexes, seasonal variation, and relationships to other sympatric marsupials. We collected 63 fecal samples from a trap grid over a six-month period and analyzed seed presence, seed number and richness, and diversity of fruit species in feces. Diversity of fruit items was estimated by the Shannon index. Results showed a high variability in fruit consumption along the seasons, but no sexual difference in consumption. Sympatric marsupial species, including D. aurita, showed substantial differences in frugivory which may be related to body size, space use and differences in the foraging behavior of such species.

Use of space by the black-eared opossum Didelphis aurita in a rural area in southeastern Brazil

Habitat fragmentation is a process that modifies the structure of an original community, favouring species that are matrixtolerant and harming species that are continuous habitatdependent (Laurance et al. 2002). The use of space by animals in fragmented environments can be affected by the arrangement of areas of suitable habitat and by the ability of the individuals to move through areas that are different from the original habitat (Gascon et al. 1999, Passamani and Ribeiro 2009). The movements of an animal in space are governed by the availability of resources in the environment and by interactions between individuals (Brown and Orians 1970). Home range is the term used to refer to the area used by an individual for its common activities (Burt 1943), and it plays a central role in studies of space use by mammals. The area used by an individual during its daily activities, or the daily home range, is a space use measurement often used in studies with neotropical small marsupials and rodents analysed with the spool-and-line technique (Delciellos et al. 2006, Almeida et al. 2008, Vieira and Cunha 2008). The black-eared opossum Didelphis aurita (Wied-Neuwied, 1826) is a generalist and opportunist marsupial that can become abundant in fragmented environments in the absence of natural predators (Fonseca and Robinson 1990). It is nocturnal, solitary and omnivorous, and plays an important role in pioneer plant seed dispersion (Caceres and Monteiro-Filho 2000). In the southern part of the state of Minas Gerais, Brazil, the development of agricultural practices and urban centres has modified the original landscape in such a way that native vegetation habitat is found in small and fragmented patches. Due to the generalist and opportunist characteristics of D. aurita, it has apparently become abundant in the remaining forest patches of the region (Silva and Pas-

Thaumasioscolex didelphidis n. gen., n. sp. (Eucestoda: Proteocephalidae) from the black-eared opossum Didelphis marsupialis from Mexico, the first proteocephalidean tapeworm from a mammal.

Thaumasioscolex didelphidis n. gen., n. sp. is described from the intestine of the black-eared opossum Didelphis marsupialis L. (Marsupialia: Didelphidae) from Los Tuxtlas, Veracruz, Mexico. The new genus differs from all proteocephalidean genera in the morphology of the scolex that is formed by 4 well separated lobes each containing 1 noncircular sucker opening laterally inside the exterolateral cavity, a large-sized body (length up to 1 m), a large number of testes, the shape of gravid proglottids that are inversely craspedote (the anterior border of a proglottid overlaps the posterior border of a preceding proglottid), eggs in groups mostly of 4-6 eggs each, and an embryophore bearing digitiform projections on its external surface. This is the first tapeworm of the Proteocephalidea, the members of which were previously reported exclusively from poikilotherm vertebrates (freshwater fishes, amphibians, and reptiles), found in a homoiotherm vertebrate.