phytes.9, 10 Investigations of hydrocarbons,ll-13 porphyrins,l2 fatty acids,14 and carbon isotopic ratios4' 15 in ancient sediments suggest basic biochemical similarities between Precambrian and extant organisms. To extend these studies, we have analyzed three fossiliferous Precambrian black cherts, ranging from about I to more than 3 billion years in age, for the presence of fossil amino acids. Our choice of amino acids for this preliminary survey is based on the following considerations: (1) Polypeptides, composed of amino acids, perform the indispensable role of mediating biochemical reactions in all known organisms; (2) analyses of Phanerozoic sediments and fossils16 17 and laboratory studies of reaction kinetics'6-18 have established that many amino acids are geochemically stable; (3) sensitive methods of amino acid analysis by cation-exchange chromatography19 and by gas chromatography20 have been developed. Materials and Mlethods.-Samples: Black cherts from fossiliferous localities of the Fig Tree Series (Early Precambrian, South Africa), the Gunflint Iron Formation (Middle Precambrian, Ontario, Canada), anld the Bitter Springs Formation (Late Precambrian, Northern Territory, Australia) were analyzed for free and combined amino acids. All three cherts are dense, black, waxy, and somewhat lustrous on freshly broken conchoidal faces, and finely and rather irregularly laminated. They are composed of about 98% cryptocrystalline quartz, and contain 0.3-0.8% organic matter; they appear to be chemical sediments of primary, rather than secondary, origin. Each of the deposits has had a mild thermal history. Bacteriumlike microfossils1 and algalike organic spheroids2 in the Fig Tree cherts, having a minimum radiogenic age of 3.1 billion years,21 constitute the oldest structurally preserved evidence of life now known from the geologic record. Pristane, phytane, and n-alkanes have been reported from the Fig Tree sediments,2 22 and the C13:C12 ratio of the indigenous organic matter, as well as the geology and mineralogy of the sedimentary sequence,', 10 suggests the presence of photosynthetic organisms. The Fig Tree chert analyzed for amino acids was collected at the same locality and horizon from which microfossils have been reported.23 The Gunflint Iron Formation has a radiogenic age of approximately 1.9 billion years.4 The fossiliferous chert analyzed in the present study was collected near Schreiber, Ontario,24 at the type-locality for many of the twelve species of microscopic plants recognized in the well-documented Gunflint microbiota.3-7 Pristane, phytane, and normal paraffins have been reported from these cherts,l3 as have C::C12 ratios for both organic and inorganic carbon.4 Black cherts of the Bitter Springs Formation, from the Amadeus Basin of central Australia, contain at least 30 species of organically preserved microorganisms;-10 chert from this fossiliferous locality25 was investigated for amino acids. Radiogenic age determinations indicate that these sediments are approximately 1 billion years old.' Reagents and analytical controls: The commercially prepared chemicals used were of reagent grade; prior to use, all reagents were tested for amino acid content. Reagent 639
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