Wastewater Treatment Bioreactors Research Articles

Large-scale comparative analysis reveals different bacterial community structures in full- and lab-scale wastewater treatment bioreactors

The activated sludge process is widely used for biological wastewater treatment due to its low cost and high efficiency. Although numerous lab-scale bioreactor experiments have been conducted to investigate the microorganism performance and mechanisms in activated sludge, understanding the bacterial community differences between full- and lab-scale bioreactors has remained elusive. In this study, we investigated the bacterial communities in 966 activated sludge samples obtained from various bioreactors, including both full- and lab-scale ones, from 95 previous studies. Our findings reveal significant differences in the bacterial communities between full- and lab-scale bioreactors, with thousands of bacterial genera exclusive to each scale. We also identified 12 genera that are frequently abundant in full-scale bioreactors but rarely observed in lab-scale reactors. By using a machine-learning method, organic matter and temperature were determined as the primary factors affecting microbial communities in full- and lab-scale bioreactors. Additionally, transient bacterial species from other environments may also contribute to the observed bacterial community differences. Furthermore, the bacterial community differences between full- and lab-scale bioreactors were verified by comparing the results of lab-scale bioreactor experiments to full-scale bioreactor sampling. Overall, this study sheds light on the bacteria overlooked in lab-scale studies and deepens our understanding of the differences in bacterial communities between full- and lab-scale bioreactors.

Life cycle costing of AnMBR technology for urban wastewater treatment: A case study based on a demo-scale AnMBR system

This study aims at assessing the economic performance of a projected full-scale anaerobic membrane bioreactor (AnMBR) for urban wastewater (UWW) treatment at ambient temperature. To this aim, data from an AnMBR demonstration plant (industrial prototype, TRL 6) was used, which was operated for 3 years treating real UWW, allowing gathering a robust set of information for scaling-up to full scale. The obtained results revealed that reactor mixing (0.056–0.124 kWh·kgCODrem-1; 34–57%), and membrane scouring (0.048–0.120 kWh·kgCODrem-1; 22–48%) were the main contributors to the total energy demand; while net energy productions between 0.210 and 0.645 kWh·kgCODrem-1 were achieved. Capital expenditure was highly influenced by UF membranes (€0.029–0.073 kgCODrem-1; 31–49%), combined heat and power technology for energy recovery (€0.012–0.023 kgCODrem-1; 8–24%), and reactor construction (€0.07–0.014 kgCODrem-1; 8–13%); while the main contributors to operating expenditure were energy requirements (€0.042–0.069 kgCODrem-1; 41–46%), membrane replacement (€0.011–0.028 kgCODrem-1; 9–17%), and discharge fee (€0.010–0.020 kgCODrem-1; 9–12%). Total annualized costs showed high variability, between €− 0.003 and 0.188 kgCODrem-1. Results presents AnMBR as a competitive technology for UWW treatment compared to conventional aerobic technologies (e.g., CAS). Membrane fouling control; hydraulic retention time; biogas requirements for reactor mixing and membrane stirring; and energy recovery efficiency were identified as key parameters for improving economic sustainability of AnMBR technology.

Unraveling microbial community by next-generation sequencing in living membrane bioreactors for wastewater treatment

This study delves into the microbial community complexity and its role in self-forming dynamic membrane (SFDM) systems, designed to remove nutrients and pollutants from wastewater, by means of the analysis of Next-Generation Sequencing (NGS) data. In these systems, microorganisms are naturally incorporated into the SFDM layer, which acts as a biological and physical filter. The microorganisms present in an innovative and highly efficient aerobic, electrochemically enhanced, encapsulated SFDM bioreactor were studied to elucidate the nature of the dominant microbial communities present in sludge and in encapsulated SFDM, patented as living membrane® (LM) of the experimental setup. The results were compared to those obtained from the microbial communities found in similar experimental reactors without an applied electric field. The data gathered from the NGS microbiome profiling showed that the microbial consortia found in the experimental systems are comprised of archaeal, bacterial, and fungal communities. However, the distribution of the microbial communities found in e-LMBR and LMBR had significant differences. The results showed that the presence of an intermittently applied electric field in e-LMBR promotes the growth of some types of microorganisms (mainly electroactive microorganisms) responsible for the highly efficient treatment of the wastewater and for the mitigation of the membrane fouling found for those bioreactors.

A novel precoated anaerobic dynamic membrane bioreactor for real domestic wastewater treatment: In-situ formation, filtration performance and characterization of dynamic membrane

Precoated dynamic membranes (PCDM), commonly formed by retaining external particles on coarse supporting meshes, can accelerate dynamic membrane (DM) formation, change the DM structure, and enhance its filtration performance. An in-situ rapid PCDM formation method via short-time and high-flux initial filtration (at 380 L/m2·h flux for 0.25 min) is proposed using anaerobic sludge as a precoating material. A precoated anaerobic dynamic membrane bioreactor (PC-AnDMBR) was operated in parallel with a self-forming AnDMBR (SF-AnDMBR) to treat domestic wastewater. Extremely low rates of transmembrane pressure (TMP) increase of 0.05 and 0.07 kPa/d were obtained during the long-term operation of the SF-AnDMBR and PC-AnDMBR, respectively. Furthermore, the PC-AnDMBR and SF-AnDMBR showed stable effluent turbidity (27.1 ± 9.44 NTU and 31.35 ± 15.96 NTU, respectively) and satisfactory COD removal (83% and 78%, respectively), which were attributed to the small pore size (<2 μm) of the DM layers. The PCDM and self-forming dynamic membrane (SFDM) with a thickness of 0.7 mm were further characterized as cake and gel layers, whereas the gel layer thickness within the PCDM was higher than that of the SFDM (0.6 mm vs. 0.2 mm). In addition, the porosities of the gel layers (2.87% and 2.95%) were lower than those of the cake layers (3.58% and 3.48%) for the PCDM and SFDM, respectively. Therefore, the compact gel layer, with more accumulated organics, contributed to over 78% of the total filtration resistance. These findings provide new insights into the mechanisms of PCDM formation and the application of AnDMBRs in wastewater treatment.

Mechanistic insights into simultaneous reversible and irreversible membrane fouling control in a vibrating anaerobic membrane bioreactor for sustainable municipal wastewater treatment

Vibrating membrane (VM) technology has shown low energy demands for in-situ antifouling enhancement in aerobic membrane bioreactors, and is expected to have greater potential for sustainable wastewater treatment applied in anaerobic membrane bioreactors (AnMBRs). However, it remains unclear how VM improves antifouling performance in AnMBRs and how the membrane fouling orignates, develops and diminishes in comparison to the conventional in-situ biogas scouring. This study reported the successful adoption of VM as a effective antifouling strategy for the vibrating AnMBR (V-AnMBR) in long-term (514 days) municipal wastewater treatment. The V-AnMBR showed superior performance, achieving 61.8%–89.4% longer filtration time, 0.68%–1.04% more organics removal and 10.7%–30.6% higher methane yields compared to the conventional biogas-scouring AnMBR (C-AnMBR). Both AnMBRs showed that membrane foulant layer (FL) was dominated in the order of colloid(FL) > EPS(FL) > SMP(FL), which could be reduced by VM to lower 10.7%–11.5% of the reversible fouling and 21.4%–26.9% of the irreversible fouling compared to biogas scouring. Furthermore, VM minimized the breakdowns of biomass typically caused by biogas scouring and reduced the release of fouling contributors from the mixed liquor, including the half of reductions of biopolymers and aromatic proteins. This work offers in-depth explanations of how membrane fouling develops in AnMBRs treating municipal wastewater, and elucidates the mechanisms of how VM achieves dual benefits on preventing foulant self-development and reducing membrane fouling contributors. Such mechanistic insights into fouling development and membrane fouling contributors would advance the understandings of antifouling mechanisms and guide next-generation sustainable AnMBR technology for mainstream wastewater treatment.

New insight into the irreversible membrane fouling in different pore-sized ultrafiltration ceramic membrane bioreactors (UCMBRs) for high-strength textile wastewater treatment

Despite great achievements in ceramic membrane bioreactor applications, membrane fouling, which decreases the permeability and separation performance of bioreactors and is associated with increased operational costs and energy consumption, remains a problem. The aim of this study was to expand our understanding of the fouling behavior in the long-term performance of ultrafiltration ceramic membrane bioreactors (UCMBRs) for high-strength textile wastewater reclamation. Using real textile wastewater effluent, the effects of ultrafiltration (UF) membrane pore sizes, cleaning strategies, and foulant distribution were systematically evaluated over more than three months of continuous operation. The results showed that UCMBR system achieved chemical oxygen demand and total nitrogen removal efficiencies as high as 91–95% and 39–43%, respectively. The high PN concentration can easily increase the viscosity of mixed liquor samples, contributing to a fouling layer on the membrane surface. In addition, the fouling layer formed on the surface of small-pore-sized ceramic UF membranes was not completely reversible but was difficult to eliminate by simple physical cleaning. Soluble extracellular polymeric substances, especially proteins and low molecular weight neutrals, remained, resulting in irreversible fouling on the UF membrane. However, saturated CO2 backwash showed great potential for enhancing the system through efficient fouling control without using environmentally unfriendly cleaning chemicals. The cake-intermediate and complete-standard models were suitable for explaining the fouling mechanism in the large- and small-pore-sized UF membranes, respectively.

Anaerobic membrane bioreactor for real antibiotic pharmaceutical wastewater treatment: Positive effect of fouling layer on antibiotics and antibiotic resistance genes removals

Anaerobic membrane bioreactor (AnMBR) is a potential candidate for high-strength pharmaceutical wastewater treatment. However, its contribution to reduce the antibiotic and antibiotic resistance genes (ARGs) is still largely unclear. Herein, this study investigated the antibiotics and ARGs removal in AnMBR for real high-strength antibiotic (levofloxacin (LEV)) pharmaceutical wastewater treatment, focusing on the positive effect of membrane fouling layers (biofilms). Results indicated that membrane biofilm has a positive effect on AnMBR treatment with improved COD and LEV removal efficiency by 12% and 10%, respectively. This might be ascribed to the enhanced biodegradation ability of the organic matter by the biofilm due to the enrichment of fermentation bacteria (Anaerobium), hydrogen-producing bacteria (Clostridium_sensu_stricto_1) and hydrogenotrophic methanogenic archaea (Methanobacterium and Methanobrevibacter). Importantly, the membrane biofilm can also effectively mitigate the ARGs (qnrS, qnrA) and class 1 integrator (intI1) dissemination and the absolute abundance of ARGs in the medium fouling (MF) biofilm effluent was 0.27 orders of magnitude lower than that of low fouling (LF) biofilm effluent. This can be explained by the variation of membrane surface becoming more hydrophobic in MF than LF biofilm with increased contact angle and more negative surface zeta potential, suggesting higher adsorption and adhesion properties. The strong correlation between extracellular polymer substance (EPS) and soluble microbial product (SMP) contents and qnrA, qnrS and intI1 further indicated the important role of EPS and SMP in membrane biofilm on ARGs reduction. Furthermore, the entrapment of ARGs potential hosts bacteria of Comamonas and Enterococcus with high biofilm formation potential also attributed to the improved ARGs removal with the increase of membrane fouling degree. Our findings suggest that AnMBR is effective for antibiotic pharmaceutical wastewater treatment and membrane biofilm has a positive effect on antibiotic and ARGs removal, which promotes the AnMBR application in the pharmaceutical industry.

Phenol biodegradation in a bioreactor considering different geometries and process parameters

AbstractIn this study, a three‐dimensional coupled lattice Boltzmann model and cellular automata platform was developed to simulate biofilm growth and phenol biodegradation as an effective and sustainable way to remove phenolic contaminants in aquatic systems. Two three‐dimensional bioreactors with cubic or spherical obstacles at varying inlet phenol concentrations and flow velocities were examined. The results showed that the cubic‐bioreactor had higher phenol reduction than the spheric‐bioreactor due to the greater effects of cubic obstacles on flow patterns. The biofilm concentration in bioreactors decreased up to 36.4% as inlet velocity increased and in the spheric‐bioreactor at the same inlet phenol concentration. The cubic‐bioreactor had the highest normalized reduction rate of 1.194 at the lowest inlet phenol concentration, while the spheric‐bioreactor had the lowest one of 0.871 at the highest inlet phenol concentration. The results proved the accuracy of the model to assess the performance of wastewater treatment bioreactors under different conditions.

Antibiotic removal from swine farming wastewater by anaerobic membrane bioreactor: Role of hydraulic retention time

This study investigated the impact of hydraulic retention time (HRT) on the removal of veterinary antibiotics by an anaerobic membrane bioreactor (AnMBR) for swine wastewater treatment. Ten commonly used antibiotics in the group of tetracyclines, fluoroquinolones, and sulfonamides were evaluated. Results show that a wide variation from 21 to 99% was observed for antibiotic removal by AnMBR regardless of the applied HRT. The methane yield increased from 0.22 L/g COD to 0.28 L/g COD as the operational HRT was prolonged from 24 to 48 h for AnMBR. Doubling the HRT value enhanced removal of most antibiotics and methane yield by 2–30% and 22–47%, respectively. The enhanced antibiotic removal in response to HRT prolongation could be positively correlated to the enrichment of the genus unidentified_Synergistaceae. Moreover, prolonging HRT increased the relative abundance of the genera unidentified_Synergistaceae, Geobacter and Methanobrevibacter, which could strengthen hydrogenotrophic methanogenesis to enhance methane yield. In addition, HRT increase reduced the ratio of protein to polysaccharide in both extracellular polymeric substances and soluble microbial products, thereby, alleviating membrane fouling in AnMBR operation.

Recent Advances in Biotechnologies for the Treatment of Environmental Pollutants Based on Reactive Sulfur Species

The definition of reactive sulfur species (RSS) is inspired by the reactivity and variable chemical valence of sulfur. Sulfur is an essential element for life and is a part of global geochemical cycles. Wastewater treatment bioreactors can be divided into two major categories: sulfur reduction and sulfur oxidation. We review the origins of the definition of RSS and related biotechnological processes in environmental management. Sulfate reduction, sulfide oxidation, and sulfur-based redox reactions are key to driving the coupled global carbon, nitrogen, and sulfur co-cycles. This shows the coupling of the sulfur cycle with the carbon and nitrogen cycles and provides insights into the global material-chemical cycle. We also review the biological classification and RSS metabolic mechanisms of functional microorganisms involved in the biological processes, such as sulfate-reducing and sulfur-oxidizing bacteria. Developments in molecular biology and genomic technologies have allowed us to obtain detailed information on these bacteria. The importance of RSS in environmental technologies requires further consideration.

Anaerobic Membrane Bioreactors (AnMBRs) for Wastewater Treatment: Recovery of Nutrients and Energy, and Management of Fouling

Anaerobic membrane bioreactor (AnMBR) technology is emerging as an alternative to conventional anaerobic treatment due to its complete biomass retention, short start-up time, high effluent quality, and small footprint. This paper provides a general overview of the application of AnMBRs for industrial and municipal wastewater treatment. The potential benefits of AnMBRs are discussed, such as the degradation of organic matter for energy production, the concentration of nutrients for subsequent reclamation, or the effective removal of organic contaminants for water reuse. To explore the technology for energy-neutral wastewater treatment, the recovery of methane, hydrogen, and ethanol is summarized, highlighting the problems of dissolution of methane in permeate and competition between sulfate-reducing bacteria and methanogens for organic matter. Recovery of water and nutrients for reuse, e.g., for algae production, is reported. Since membrane fouling remains a challenge in membrane operation and leads to increased operation and maintenance costs, methods to reduce fouling are highlighted. Future research prospects related to the application of AnMBR in resource recovery plants and fouling management are emphasized.

A novel reduced graphene oxide/polypyrrole conductive ceramic membrane enhanced electric field membrane bioreactor: Mariculture wastewater treatment performance and membrane fouling mitigation

A novel electric field membrane bioreactor (EMBR) for mariculture wastewater treatment utilizing reduced graphene oxide/polypyrrole ceramic membrane (rGO/PPy CM) was constructed and compared with MBRs using CM support and rGO/PPy CM. EMBR (rGO/PPy) obtained the highest pollutant removal rates (84.99% for TOC, 85.98% for NH4+-N), the lowest average membrane fouling rate (2.42 kPa/d) and pollutant adhesion performance by characterization. Meanwhile, the specific fluxes of characteristic foulants in EMBR were enhanced, and the total resistances were reduced by 8.12% to 62.46%. The underlying mechanisms included reduced attraction energy and improved electrostatic repulsion between contaminants in EMBR and membrane by the extended Derjaguin–Landau–Verwey–Overbeek (XDLVO) theory, DLVO model and force analysis. Therefore, this study complemented the understanding of antifouling effect and mechanism in EMBR by interaction energy and force analysis of characteristic pollutants. These findings also provided new insights into the application of EMBR for mariculture wastewater treatment.

Temporal assembly patterns of microbial communities in three parallel bioreactors treating low-concentration coking wastewater with differing carbon source concentrations

Carbon source is an important factor of biological treatment systems, the effects of which on their temporal community assembly patterns are not sufficiently understood currently. In this study, the temporal dynamics and driving mechanisms of the communities in three parallel bioreactors for low-concentration coking wastewater (CWW) treatment with differing carbon source concentrations (S0 with no glucose addition, S1 with 200 mg/L glucose addition and S2 with 400 mg/L glucose addition) were comprehensively studied. High-throughput sequencing and bioinformatics analyses including network analysis and Infer Community Assembly Mechanisms by Phylogenetic bin-based null model (iCAMP) were used. The communities of three systems showed turnover rates of 0.0029∼0.0034 every 15 days. Network analysis results showed that the S0 network showed higher positive correlation proportion (71.43%) and clustering coefficient (0.33), suggesting that carbon source shortage in S0 promoted interactions and cooperation of microbes. The neutral community model analysis showed that the immigration rate increased from 0.5247 in S0 to 0.6478 in S2. The iCAMP analysis results showed that drift (45.89%) and homogeneous selection (31.68%) dominated in driving the assembly of all the investigated microbial communities. The contribution of homogeneous selection increased with the increase of carbon source concentrations, from 27.92% in S0 to 36.08% in S2. The OTUs participating in aerobic respiration and tricarboxylic acid (TCA) cycle were abundant among the bins mainly affected by deterministic processes, while those related to the metabolism of refractory organic pollutants in CWW such as alkanes, benzenes and phenols were abundant in the bins dominated by stochastic processes.

Fabrication and characterization of different braid‐reinforced PVC hollow fiber membranes to use in membrane bioreactor for wastewater treatment

AbstractIn this research, polyvinyl chloride (PVC) polymer was applied for the first time in the fabrication of braid‐reinforced hollow fiber membranes for ultrafiltration applications. Different concentrations of PVC solutions were cast on tubular braids and coagulated by the phase inversion method in a pure water bath to produce membranes with various porosities and pore sizes. The characterizations such as scanning electron microscopy, overall porosity, contact angle and mean pore size were done to understand membrane morphology. The antifouling property of membranes was explained using bovine serum albumin (BSA). The contact angles of membranes were increased from 64.9° ± 2.2 to 72.8° ± 2.1, while the overall porosity decreased from 43.8 ± 1.2% and 19.6 ± 3.3% for 11 wt% and 17 wt% PVC membranes, respectively. Permeate flow decreased due to decreased pore size of membranes as a result of increased PVC content. Besides, the flux recovery ratio, reversible and irreversible fouling studies showed that better antifouling characteristics observed with increasing of PVC content. A high removal efficiency of 87.2% for BSA was also demonstrated by the optimal membrane (15 wt% PVC), along with an increased flux recovery ratio of 96.6%. After investigating the rejection of COD in a membrane bioreactor setup, all membranes revealed that they were higher than 98%.

Macroalgal biochar synthesis and its implication on membrane fouling mitigation in fluidized bed membrane bioreactor for wastewater treatment

The intensification of biochar into fluidized bed membrane bioreactor was investigated to mitigate membrane fouling. Different biochars from algal biomass were produced and used as biomaterials for wastewater treatment. In this study, different macroalgal biochar was synthesized at different pyrolysis temperatures and characterized using Scanning Electron Microscopy (SEM), X-ray Diffraction (XRD), Brunauer Emmett-Teller (BET) and Fourier transform infrared spectroscopy (FTIR) techniques to implicate their effect on membrane fouling reduction in fluidized bed membrane bioreactor. The combined effect of macroalgal biochars and biocarriers with gas sparging was evaluated for fouling mitigation. Macroalgal biochar curtailed membrane fouling effectively at low gas sparging rate. Transmembrane pressure (TMP) was reduced to 0.053 bar; under the fluidization of biochar-650 and biocarriers with gas sparging; from 0.27 bar (gas sparging only). Combined effect of gas sparging, biocarriers and biochar-650 instigated 92.1% fouling reduction in comparative to gas sparging alone. Mechanical scouring driven by biocarriers could reduce fouling due to removing surface deposit of foulants from membrane surface effectively and biochar can efficiently adsorb foulants because of its active functional groups resulting in reduction of colloidal fouling. The addition of divalent ions (Ca2+) further enhanced the fouling reduction in fluidized bed membrane bioreactor.

Genome-centric metagenomic insights into the role of Chloroflexi in anammox, activated sludge and methanogenic reactors

BackgroundThe phylum Chloroflexi is highly abundant in a wide variety of wastewater treatment bioreactors. It has been suggested that they play relevant roles in these ecosystems, particularly in degrading carbon compounds and on structuring flocs or granules. Nevertheless, their function is not yet well understood as most species have not been isolated in axenic cultures. Here we used a metagenomic approach to investigate Chloroflexi diversity and their metabolic potential in three environmentally different bioreactors: a methanogenic full-scale reactor, a full-scale activated sludge reactor and a lab scale anammox reactor.ResultsDifferential coverage binning approach was used to assemble the genomes of 17 new Chloroflexi species, two of which are proposed as new Candidatus genus. In addition, we recovered the first representative genome belonging to the genus ‘Ca. Villigracilis’. Even though samples analyzed were collected from bioreactors operating under different environmental conditions, the assembled genomes share several metabolic features: anaerobic metabolism, fermentative pathways and several genes coding for hydrolytic enzymes. Interestingly, genome analysis from the anammox reactor indicated a putative role of Chloroflexi in nitrogen conversion. Genes related to adhesiveness and exopolysaccharides production were also detected. Complementing sequencing analysis, filamentous morphology was detected by Fluorescent in situ hybridization.ConclusionOur results suggest that Chloroflexi participate in organic matter degradation, nitrogen removal and biofilm aggregation, playing different roles according to the environmental conditions.

Hybrid bioreactor built-in with fixed bio-carriers for denitrification with low C/N ratio: Hydrodynamic optimization and microbial divergence

Hydrodynamics played an important role in the design and operation of bioreactors for wastewater treatment. In this work, an up-flow anaerobic hybrid bioreactor built-in with fixed bio-carriers was designed and optimized using computational fluid dynamics (CFD) simulation. The results indicated that the flow regime involving with vortex and dead zone was greatly affected by the positions of water inlet and bio-carrier modules. The ideal hydraulic features were obtained when the water inlet and bio-carrier modules located 9 cm and 60 cm above the bottom of reactor. Using the optimum hybrid system for nitrogen removal from wastewater with low carbon-to-nitrogen ratio (C/N = 3), the denitrification efficiency could reach 80.9 ± 0.4%. Illumina sequencing of 16S rRNA gene amplicons revealed that the microbial community divergence occurred among the biofilm on bio-carrier, the suspended sludge phase and the inoculum. Especially, the relative abundance of denitrifying genera Denitratisoma in the biofilm of bio-carrier reaches 5.73%, 6.2 times higher than that in the suspended sludge, implying the imbedded bio-carrier was conductive to enrich the specific denitrifiers to polish the denitrification performance with low carbon source. This work provided an effective method for the design optimization of bioreactor based on CFD simulation, and developed a hybrid reactor with fixed bio-carrier for nitrogen removal from wastewater with low C/N ratio.

Performance evaluation of submerged membrane bioreactor for municipal wastewater treatment: Experimental study and model validation with GPS‐X software simulator

AbstractIn this study, flat sheet submerged MBR (FS‐SMBR) was investigated under 6 and 4 h hydraulic retention times (HRT) and 40 days solid retention time with real municipal wastewater. The implementation of the treatment system was evaluated in terms of physico‐chemical and microbial analysis as well as sludge characterization. The permeate of FS‐SMBR complied with the standards for reuse and non‐detectable levels of faecal coliform was achieved at 6 and 4 h HRTs, with pore‐blocking resistances (Rt) of 28 × 1011 and 40 × 1011/m, respectively. Examining another dominant factor, that is, sludge viscosity, the best results were acquired using HRT 4 h for domestic wastewater. Furthermore, the results obtained were validated and calibrated using the GPS‐X software simulator, and the results of modelled data were completely matched with the experimental data under the same conditions.

Progress on the Development of Techniques to Remove Contaminants from Wastewater: A Review

The continuous depletion of fresh drinking water resources throughout the world has increased the necessity for the development of new techniques for wastewater treatment and recycling process. Domestic, industrial and agricultural wastes are major sources of aquatic contamination all over the world. Before discharging wastewater into natural water bodies, it must be treated. Apart from primary, secondary and tertiary treatment, membrane separation techniques and electrochemical membrane bioreactors for wastewater treatment have all been studied in this review paper. The electrochemical membrane bioreactor (EMBR) system has a lot of potential for treatment of wastewater and energy recovery. These novel EMBR techniques give feasible solutions for renewable treatment of wastewater and resource resumption.

The impact of sunlight on fouling behaviors and microbial communities in membrane bioreactors

Membrane fouling is a significant obstacle to applying membrane bioreactors (MBRs) for wastewater treatment. Here we report the impact of sunlight irradiation on membrane fouling, biopolymers, signal molecules, and microbial communities in MBRs. The degradation of signal molecules, which induce membrane biofouling, occurred through solar photolysis in batch tests. However, MBR sludge exposed to sunlight exhibited different biological behaviors creating more soluble microbial products (SMP) and signal molecules (particularly autoinducer-2). Cell lysis and deflocculation occurred when the MBR mixed liquor was exposed to sunlight. MBR fouling rates coincided with the temporal concentration profiles of SMP and signal molecules. Sunlight caused drastic changes in the MBR microbial community, stimulating the preferential growth of specific bacteria (e.g., Deinococcus Runella, Flavitalea, Glaiimonas, and Rurimicrobium). The nonmetric multidimensional scaling analysis of the MBR community structures with and without sunlight irradiation showed two distinct microbial community clusters and their reversibility. Network analysis based on Spearman's rank correlations revealed that with sunlight irradiation, fouling rates had significant positive connections with SMP proteins and the Flavitalea genus. The findings of this study demonstrate that sunlight is a considerable factor affecting membrane fouling and microbial ecology in MBRs, needing shade for fouling mitigation and sustainable operation.