Granular hydrogels, composed of densely packed microgels, are an emerging class of injectable microporous scaffolds that provide interstitial porosity for endogenous cell recruitment and tissue repair. However, weak bonding interactions between constituent microgels compromise the mechanical integrity of these biomaterials, limiting their scope and effectiveness for in vivo applications where structural support is required. To address this challenge, we introduce a new bioinspired stabilization method and a novel class of regenerative biomaterial: coagulative granular hydrogels, assembled from thrombin-functionalized gelatin methacryloyl microgels. The surface-bound thrombin is enzymatically active and catalyzes the conversion of fibrinogen into a fibrin hydrogel that extends throughout the interstitial voids of the granular hydrogel. This secondary network acts as a biological glue to stabilize the granular hydrogel, yielding shear and compressive properties comparable to bulk hydrogel controls. Furthermore, the interstitial fibrin network provides a favorable microenvironment for the adhesion, proliferation, and invasion of endothelial cells, highlighting the potential of the biomaterial to support endogenous tissue repair. Subcutaneous injection in mice showed that the coagulative granular hydrogelspreserved structural integrity and supported fibrin deposition, cell infiltration, and collagen remodeling in vivo. Future work will adapt this technology to other biomaterials and validate its performance for different tissue repair applications.

Abstract Humans struggle to design effective underwater adhesives, yet they are essential for numerous technical and biomedical applications. In contrast, biological organisms—most notably mussels—have evolved glues that excel in aquatic environments. While researchers have drawn inspiration from mussels, the resulting materials remain limited by a poor understanding of the native mussel glue formation process. Here, the contents of glue secretory vesicles extracted from mussels are investigated, revealing fluid condensates comprised of the various protein components of the glue. Proteomic analysis confirms the presence of several previously unconfirmed glue proteins in the vesicles, as well as several enzymatic components that may play a role in regulating glue oxidation and cross‐linking. Mimicking vesicle conditions, a method is developed to maintain the vesicle proteins in a reconstituted bulk fluid condensate, enabling in vitro analysis and hypothesis testing. A combination of proteomics, vibrational spectroscopy, and nanomechanical adhesion testing reveals the crucial contributions of several physicochemical factors (e.g., pH, sulfate and vanadium ions, sulfhydryls) for the processing and performance of mussel glues as they transition from fluid condensates to microporous solid glues. These findings are crucial for understanding biological glues and the development of next generation bio‐inspired underwater adhesives.

Biological glue (bioglue) can noninvasively seal and reattach wound tissue outside the hospital, which has been extensively studied recently. Nevertheless, the commercial bioglue lacks constant oxygen supply, antioxidation, and anti-inflammation, three other key factors for bacteria-infected wound healing in plateau areas. Herein, a multifunctional bioglue (LA-DESP/ε-PLL@Chlorella) is simply fabricated by encapsulating Chlorella into a matrix composed of α-lipoic acid (LA), α-lipoic acid sodium (LANa), and ε-polylysine (ε-PLL). In vitro studies depicted that the LA-DESP/ε-PLL@Chlorella bioglue exhibits good adhesion, oxygen delivery, antioxidant, antibacterial, antibiofilm, and anti-inflammatory activities. Furthermore, in vivo experiments show that LA-DESP/ε-PLL@Chlorella bioglue could eradicate bacteria, reduce inflammation, and promote the healing of bacteria-infected wound. This multifunctional bioglue represents a promising concept and alternative therapeutic option for accelerating the healing of bacteria-infected wounds.

A novel flocculant based on "happy molecules" for the efficient removal of NSAIDs and NOM complexes: Role of parallel π-π stacking force.

BackgroundLead extraction is a complex and potentially hazardous procedure. Most lead extractions use an "over-the-lead" cutting tool (sheath), to separate the electrode from surrounding vessels and cardiac tissue. However, these tools pose significant risks, including collateral damage and major bleeding.ObjectiveWe introduce a novel, "minimally invasive" approach for lead extraction. Vibrations delivered through a locking stylet detach the electrode from the surrounding tissue without using cutting tools.Methods●Bench Experiments:A three-dimensional model of central veins was developed. Using a strong biological glue, the pacing lead was attached to a silicone sphere representing the heart, which was suspended in air by a metal spring. Vibration waves of varying frequencies and amplitudes were applied to the lead to determine the optimal detachment parameters.●Animal Studies:Five sheep with ICD or pacemaker leads implanted in the atrium and ventricle underwent lead extraction using the vibration-based device, 3 to 7 months post-implantation. Additionally, four beagle dogs with ventricular pacing leads implanted for 4 to 6 years underwent extraction with this device.●First-in-Human Study:Ten consecutive patients scheduled for lead extraction, per current guidelines, were enrolled in this study. Inclusion criteria were ages 18 to 80 and lead indwelling times of 1 to 10 years. Procedures were conducted in two countries at two tertiary academic hospitals. All patients were monitored one month post-procedure.ResultsAll leads in the animal and human studies were successfully extracted using vibration-based method, with physical parameters defined by bench experiments. Cardio-respiratory monitoring revealed no significant disturbances during the procedures.●Animal Studies:Of the 9 leads extracted (7 pacing leads, 2 ICD leads), no significant damage to the heart or vessels was observed through anatomical and histological examination.●First-in-Human Study:In ten patients, sixteen leads (9 pacing leads, 5 ICD leads, and 2 CS lead) were extracted, with an average indwelling time of 4.2±1.9 years (range: 2–7.5 years).Six patients had device and lead infections, and four required system upgrades or replacement of malfunctioning leads. Extraction times ranged from 4 to 176 seconds, with a median time of 15 seconds. In three patients, additional short vibrations were applied after distal detachment, to aid in pulling the lead through proximal narrowing. In-hospital and one-month follow-ups indicated no significant adverse effects from using the experimental device. No patients required additional procedures or unplanned hospitalizations.ConclusionThis new lead extraction device offers a fast and straightforward procedure using vibrations via a locking stylet, eliminating the need for an external cutting sheath. Initial experiments demonstrate high efficiency and safety with this innovative approach.Seven year ICD Lead and Vibration Handle

Dandruff is a common skin condition affecting up to half of the world's population. Symptoms include pruritus, inflammation, and flaking. It is thought to be in part due to microbe dysbiosis. This paper aims to discuss the relationship between dandruff and Cutibacterium acnes (C. acnes). It will also explore the potential role of C. acnes's biofilm in the formation of the biological glue that sticks dandruff flakes together. Citation: Scott E, Burkhart C. A review of the role of Cutibacterium acnes and its biofilm in dandruff pathogenesis. J Drugs Dermatol. 2025;24(6):566-569. doi:10.36849/JDD.8648R1.

The aim of this improvement collaborative is to explore the variation in care within and between Flemish hospitals in preoperative assessment, surgical indications, perioperative management and surgical technique for ventral mesh rectopexy (VMR). This observational, cross-sectional multicentre study was performed in 14 Flemish hospitals. Twenty consecutive patients per hospital undergoing primary VMR in 2022 were included. Quality of care was assessed via predefined perioperative disease-specific quality indicators (QIs) by means of structured questionnaires. Data were collected from electronic patient files. A total of 280 patients were included. All patients were female and their mean age was 62 ± 14 years. Significant intra- and interhospital variation was observed in preoperative work-up, indications, operative technique and postoperative management. Total rectal prolapse was the indication for VMR in only 17.5% of the patients. The surgical approach was minimally invasive in all cases, with 40% via a robotic and 60% a laparoscopic approach. Fifteen per cent of patients had mechanical bowel preparation. All centres used a synthetic polypropylene mesh to perform a VMR, and in 85.6% (n = 238) of all patients a lightweight mesh was used. Diverging practices were noted as to type of mesh fixation to the rectum. In one third of patients a nonresorbable suture was combined with biological glue (n = 89, 31.8%). The overall mean length of stay was 2.1 (± 2.7) days. Only 3% of the procedures were performed as same day discharge, 47% of the patients remained for 1 day and 50% for ≥2 days. Only four patients were readmitted within 30 days after surgery. This study shows a significant variation in the perioperative management and surgical technique for VMR between hospitals, ongoing controversies and a lack of standardization. This collaborative can serve as a structured feedback tool to define minimum QIs and minimum outcome reporting parameters. Consensus building and adherence to evidence-based guidelines should reduce variation in care processes and lead to improved patient outcomes.

Enterocutaneous (EC) fistula incidence has been increasing in China, along with increases in the volume and complexity of surgeries. The conservative treatment strategy has been analyzed to improve the treatment outcomes for patients with EC fistulas and reduce the need for reoperation. To analyze the clinical data of patients undergoing conservative treatment for EC fistulas and identify the factors that promote self-healing. These findings provide a reference for improving the clinical cure rate of EC fistulas with conservative treatment. The clinical data of 91 patients with EC fistulas who underwent conservative treatment were collected. The relationships between the cure rate and characteristics such as age, sex, body mass index, albumin level, primary disease, cause of the fistula, location of the fistula, number of fistulas, nature of the fistula, infection status, diagnostic methods, nutritional support methods, somatostatin therapy, growth hormone therapy, and fibrin glue therapy were analyzed. A comparison of the basic patient characteristics between the two groups revealed statistically significant differences in primary disease (P = 0.044), location of the fistula (P = 0.006), number of fistulas (P = 0.007), and use of adhesive sealing (χ 2 = 12.194, P < 0.001) between the uncured and cured groups. The use of fibrin glue was a significant factor associated with a cure for fistulas (odds ratio = 5.459, 95%CI: 1.958-15.219, P = 0.01). The cure rate of patients with a single EC fistula can be effectively improved via conservative treatment combined with the use of biological fibrin glue to seal the fistula.

The Role of Cutibacterium acnes Biofilm as a Biological Glue in Acne and Dandruff: Current Insights and Future Directions.

ObjectivesDespite the advances in medicine, aortic dissection remains a cardiac surgery emergency with high mortality and morbidity rates. This study examined the effects of the Glue + Felt technique, which uses biological glue and felt to repair the proximal anastomotic site, on the outcomes of patients with acute type A aortic dissection.MethodsA total of 108 patients who underwent surgery for acute type A aortic dissection at our clinic between 2007 and 2020 were included in the study. The patients were divided into two groups: the "Glue + Felt Technique" and the "Bentall-De Bono" groups, based on the surgical technique used for the aortic root. The effects of these two techniques on the development of intraoperative and postoperative complications and survival rates were statistically analyzed.ResultsThe Glue + Felt technique was used for 76 patients, while the Bentall-De Bono technique was used for 32 patients. The Kaplan–Meier analysis revealed significant differences in survival rates between the two groups over the entire follow-up period, both with and without propensity score matching (p < 0.001 and p = 0.02, respectively). However, no significant differences were observed in comparisons beyond the first 30 days of follow-up, either with or without propensity score matching (p = 0.573 and p = 0.561, respectively). The main factors contributing to this difference were the duration of cardiopulmonary bypass and aortic cross-clamp time (p < 0.05). During the average follow-up period of 46.2 ± 31.6 months, no re-intervention was required in patients from the Glue-Felt technique group.ConclusionsThe mortality rate in aortic dissection surgery is higher with more extensive surgical intervention as the duration of cardiopulmonary bypass and aortic cross-clamp time increases. Repairing the lumen and reducing operation time in suitable patients using the Glue-Felt technique for the proximal anastomotic site positively impacts postoperative complications and improves in-hospital and 30-day survival rates, without increasing long-term re-intervention rates.

We reviewed the available literature on patients undergoing aortic repair for acute type A aortic dissection (ATAAD) with either aortic root preservation (RP) or root replacement (RR). Original research studies that evaluated short- and mid-term hemostatic properties of RP versus RR groups were identified, from 2000 to 2024. Intraoperative transfusions of red blood cells (RBCs), reoperation for bleeding, strategy of hemostatic sealing of the anastomosis in root repair following the reapproximation of the dissected layers of the aortic wall (with/without biological glue), and operative mortality were the primary endpoints. Postoperative morbidity and overall and reoperation-free survival at one and five years were the secondary endpoints. A sensitivity analysis was performed using the leave-one-out method. Ten studies were included in the qualitative and quantitative synthesis, incorporating data from 6850 patients (RP: 4389 patients; RR: 2461 patients). Root preservation demonstrated a lower median transfusion of RBCs (WMD: -1.00; 95% CI: -1.41, -0.59; p < 0.01) and incidence of reoperation for bleeding compared to root replacement (OR: 0.67; 95% CI: 0.58, 0.77; p < 0.01). The majority of studies did not use biological glue in root repair to avoid the risk of an anastomotic pseudoaneurysm. No difference was found regarding postoperative morbidity, along with mid-term overall and reoperation-free survival. Root preservation without the use of biological glue during aortic repair is associated with enhanced hemostatic traits compared to the root replacement approach. A future well-designed Randomized Controlled Trial should further validate our outcomes.

Background/Objectives: The main purpose of this study is to determine, by anterior segment optical coherence tomography (AS-OCT), the anatomical characteristics, both preoperatively and postoperatively, that correlate with a higher rate of pterygium recurrence after surgery with exeresis and conjunctival autograft with biological glue. Methods: A total of 50 eyes which were listed for primary pterygium surgery at an ophthalmology tertiary centre were treated with standard pterygium excision and a conjunctival autograft with tissue glue. Ten variables were measured with AS-OCT (Casia 2; Tomey Corp., Nagoya, Japan) during six control visits with all patients. Finally, statistical analysis was performed using SPSS (SPSS stadistics®, IBM®, version 21.0.0.0) for descriptive variables and R-project (The R foundation©, version 3.0.2) for the rest of the analyses, including a descriptive analysis and an inferential analysis studying prognostic factors of recurrence and their predictive capacity. Results: Among the 50 patients who underwent surgery, recurrence was detected in 8 cases (rate 16%; 95% CI: 5.8-26.2%). Most cases (n = 6) were detected 3 months after surgery. The pattern of recurrences was atrophic in two thirds of the cases; none required reintervention. Preoperative total conjunctival thickness at 3 mm was significantly increased in patients who developed recurrence. One week after surgery, epithelial and stromal thickness at 1 mm and total thickness at 3 mm proved to be useful for predicting recurrence. Both models have significant discriminant capacity. Conclusions: By imaging the graft with AS-OCT preoperatively and 7 days after surgery, the risk of future recurrence can be predicted.

Abstract Aim The fixation of the mesh in transabdominal preperitoneal inguinal hernia repair (TAPP) and the closing of the peritoneal flap are key parts of the operation. This is accomplished with sutures or tackers. The use of biologic glue is an acceptable alternative with the possible advantages of being safer and less painful than the other options. Its use however poses its own set of challenges. Materials and Methods 24 patients underwent TAPP repair for inguinal hernia, using biologic glue to fix the mesh and close the peritoneal flap. The practical challenges of its use were studied. Results No problems were encountered in the fixation of the mesh. In the closing of the peritoneal flap, tackers were used as an adjunct in the first 10 cases. After that, the operation was completed only using glue. Conclusions Various conclusions were drawn from the use of glue. First, it works best in a dry environment and with a lightweight microporous mesh, so that the glue can seep through the pores. Second it will not “fix” large creases of the mesh, although this also means a suboptimal positioning of the mesh. Third, it requires gentle moves during withdrawal, as the tip can stick to the tissues, and appliance of a wet gauze after every use, as the clotted glue can block the tip. All in all, after a learning curve period, it can be a safe and effective alternative to sutures and tackers in TAPP.

INTRODUCTION: Brachial plexus injuries are very much devastating and lead to significant socioeconomic and mental burden to the affected person and his family. In the early days of 20th century many authors were in favour of conservative management even the amputation of affected limb. But in later decades due to development in technological advancement of operating microscope, intraoperative electrophysiological monitoring, biological tissue glue etc. the results of surgical repair are beyond the imagination that could be done previously. METHODS: Our study is a Retrospective observational analytical study in which we included patients of Pan brachial plexus injury operated under the Department of Neurosurgery from April 2011 till February 2023 (excluding covid period from March 2020 to September 2021) at apex trauma center AIIMS Delhi, India. Demographic details, mode of injury, surgery performed and outcome were analyzed. Data was extracted from patient’s casualty, ICU and OT records and analyzed using SPSS version 29.0.0.0. Analysis of outcome was taken in terms of improvement in motor power and quality of life. RESULTS: There were 113 patients diagnosed to have Pan brachial plexus injury by clinical examination and supported with brachial plexus MRI, NCV and EMG of the affected limb. 24 (21.24%) cases underwent Neurolysis only, 17 (15.04%) cases underwent Supraclavicular neurotization only, 24 (21.24%) cases were operated by infraclavicular neurotization and 48 (42.48%) patients were operated by supraclavicular and infraclavicular neurotization both. Respectively 25%, 23.52%, 25% and 35.42% cases had motor and sensory improvement and were able to perform few of their daily activities with lesser support. CONCLUSIONS: In our study at least 25% Patients of Pan brachial plexus injury after surgery got benefited in their routine activity and quality of life thus lesser affected in socioeconomic and psychological front.

Aims Identify risk factors associated with recurrent GV(gastric varices ) bleeding at 6 weeks following biologic glue injection and evaluate its impact on mortality.

Favorable effects of open surgery on patients with extensive skull base osteoradionecrosis through a personalized sequential approach: A case series

Unusual Use of Biological Glue in Complicated Acute Type A Aortic Dissection

Arteriovenous malformations (AVMs) are the rarest and most serious of vascular malformations. Radiologically, the lesion consists of a vascular "nidus", a cluster of abnormal vessels fed and drained by one or more arteries and veins dilated by increased flow. The treatment of peripheral AVMs is complex, and the choice of treatment is determined by multidisciplinary consultation, since several options are available. Embolization plays an important role in the therapeutic arsenal, and particularly the one combined with surgery.

This paper shows the combined effect of throughflow and gravity modulation on the stability of Oldroyd-B nanofluid filled in Hele-Shaw cell. Nanofluid compared to the base fluid has higher thermal conduction. The thermal conductivity of nanofluid increased and thus increases the amount of energy transferred. The Oldroyd-B fluid model is important because of its numerous applications such as production of plastic sheet and extrusion of polymers through a slit die in polymer industry, biological solution pant tars glues, etc. In linear stability analysis, we found the expression of the critical Hele-Shaw Rayleigh number by using the normal mode method. Two-term Fourier series method is used for non-linear stability analysis and is also considered the Brinkman model for flow of nanofluid in Hele-Shaw cell. In linear stability analysis, we observed that there is no effect of Oldroyd-B nanofluid, which means that Deborah number (λ1) and retardation parameter (λ2) do not affect the stability analysis. Oldroyd-B nanofluid is similar to ordinary nanofluid in linear analysis. In non-linear analysis, Deborah number, retardation parameter, throughflow, gravity modulation, and Hele-Shaw number play a major role in heat/mass transfer. Enhancement in both heat/mass transfer in the system while increasing throughflow and Deborah number. An increment in Hele-Shaw number (Hs), decreases heat/mass transfer in the system.

Chirurgie du ptérygion par greffe conjonctivale sans suture ni colle biologique : étude préliminaire au centre hospitalier universitaire de Cocody à Abidjan