Bioactive Research Articles

Interaction of VVO2 − Hydrazonates with lysozyme

Vanadium compounds (VCs) exhibit a broad range of pharmacological properties, with their most significant medical applications being in the treatment of cancer and diabetes. The therapeutic effects and mode of action of VCs may be associated with their ability to bind proteins and, consequently, understanding the VC–protein interaction is of paramount importance. Among the promising VCs, the VVO2 complex with the aroylhydrazone furan-2-carboxylic acid (3-ethoxy-2-hydroxybenzylidene)hydrazide, hereafter denoted as VC1), deserves attention, since it exhibits cytotoxicity against various cancer cell lines, including HeLa. The interaction between VC1 and a new VC1 analogue, denoted as VC2 (the dioxidovanadium(V) complex with (E)-N′-(1-(2-hydroxy-5-methoxyphenyl)ethylidene)furan-2-carbohydrazide), and hen egg white lysozyme (HEWL) was examined by UV–vis spectroscopy, fluorescence, circular dichroism, and X-ray crystallography. The interaction of VC1 and VC2 with HEWL does not alter the protein secondary and tertiary structure. Crystallographic studies indicate that the two metal complexes or V-containing fragments originating from these molecules bind the protein via non-covalent interactions. Furthermore, when bound to HEWL, two VC1 molecules and two VC2 molecules form a supramolecular association stabilized by stacking interactions. This type of interaction could favour the binding of similar compounds to proteins and affect their biological activity.

Exploration of Agrocybe aegerita mushroom polysaccharide-polyphenolic complex: Functional properties, binding efficiency, and biological activities.

Exploration of Agrocybe aegerita mushroom polysaccharide-polyphenolic complex: Functional properties, binding efficiency, and biological activities.

Calliloboapins A-L, diterpenoids from the branches and leaves of Callicarpa loboapiculata and their biological activities.

Calliloboapins A-L, diterpenoids from the branches and leaves of Callicarpa loboapiculata and their biological activities.

Uncovering the in vitro antibiofilm potential of Dittrichia graveolens silver nanoparticle against infectious agents

Dittrichia graveolens (L.) Desf., an aromatic plant, is used in various treatments in popular medicine. Especially its methanolic and aqueous extracts have many biological activities. Nanotechnology, which has pioneered important research in recent years, has also begun to be preferred in the field of health. Based on this, we aim to investigate the effect of silver nanoparticle synthesized from Dittrichia graveolens on antimicrobial and antibiofilm. We tried to destroy the biofilm structures responsible for antibiotic resistance in bacteria with environmentally friendly nanoparticles. The antimicrobial activity of AgNPs obtained through green synthesis, was evaluated by the liquid medium microdilution method. MIC (minimum inhibitory concentration) values of AgNPs for Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae strains were determined by the microdilution method in 96-well ELISA plates. The effect of AgNPs on biofilm was performed using the crystal violet method in 96-well flat-bottom microplates. The MIC values of the four standard strains were determined to be 128 μg/mL. All standard strains showed antibiofilm effects for every concentration of AgNPs. The lowest concentration of AgNPs for inhibition the biofilm was detected as about 90% at 16 μg/mL. It was concluded that green synthesized AgNPs was effective on bacteria. In addition, this is the first study on this subject. This study may be an innovative approach to the scientific world from a herbal and bacterial perspective.

How wine blending influences their biological activity: A case study of Cabernet Sauvignon and Merlot coupages.

How wine blending influences their biological activity: A case study of Cabernet Sauvignon and Merlot coupages.

Synthesis, antiproliferative activity, and biological profiling of C-19 trityl and silyl ether andrographolide analogs in colon cancer and breast cancer cells

Synthesis, antiproliferative activity, and biological profiling of C-19 trityl and silyl ether andrographolide analogs in colon cancer and breast cancer cells

Carotenoids Improve Obesity and Fatty Liver Disease via Gut Microbiota: A Narrative Review.

Carotenoids are natural micronutrients found in plants and microorganisms, but not synthesized by animals. Carotenoids show various biological activities, including antioxidant properties, regulation of cell growth, and modulation of gene expression and immune responses. The rising global incidence of fatty liver disease (FLD) and obesity highlights the importance of carotenoids in chronic progressive conditions. Gut microbiota (GM) dysbiosis is associated with the development and progression of obesity and FLD due to the effects of metabolites such as lipopolysaccharide (LPS), bile acids (BAs), and short-chain fatty acids (SCFAs). Furthermore, GM may affect intestinal barrier integrity. This review evaluates the potential impact of carotenoids on GM and intestinal barrier function, and their subsequent effects on obesity and FLD. We searched through a wide range of databases, such as Web of Science, Scopus, EMBASE, and PubMed, to collect data for our non-systematic review of English literature. Carotenoids such as lycopene, zeaxanthin, fucoxanthin, capsanthin, astaxanthin, and lutein can regulate GM composition and improve obesity and FLD by affecting energy expenditure, food intake, lipid profile, liver fat deposition, liver enzymes, inflammatory markers, glucose homeostasis, and bile acids. These carotenoids improve obesity and FLD through GM metabolites such as SCFAs and LPS. Our findings show that dietary supplementation of lycopene, zeaxanthin, fucoxanthin, capsanthin, astaxanthin, and lutein can positively affect obesity and FLD by regulating GM and intestinal barrier integrity.

Curvilinear regression analysis and ranking of migraine treatment drugs using degree-based topological indices and the WASPAS method.

Curvilinear regression analysis and ranking of migraine treatment drugs using degree-based topological indices and the WASPAS method.

Reduced soil ecosystem multifunctionality is associated with altered complexity of the bacterial-fungal interkingdom network under salinization pressure.

Reduced soil ecosystem multifunctionality is associated with altered complexity of the bacterial-fungal interkingdom network under salinization pressure.

Research progress in the extraction, purification, structural features, biological activities, and structure-activity relationships from Prunella vulgaris polysaccharides

Research progress in the extraction, purification, structural features, biological activities, and structure-activity relationships from Prunella vulgaris polysaccharides

Biological Activities of Aurones: A Brief Summary

Aurones are structural isomers of flavones and flavonols with the basic C6–C3–C6 skeleton arranged as (Z)-2-benzylidenebenzofuran-3(2H)-one, which contain an exocyclic carbon-carbon double bond bridging the benzofuranone and phenyl rings. In aurone, a chalcone-like group is closed into a 5-membered ring instead of the 6-membered ring more typical of flavonoids, which forms the core for a family of derivatives that are known collectively as aurones. As a kind of flavonoids, aurones are widely distributed in many plants which provide yellow color to some popular ornamental flowers. For a long time aurones had not got enough attention, while in recent years, finally this chemical is coming into researchers' view. As the secondary metabolite in the family of flavonoids, aurones displayed various biological activities, including antioxidant, antiparasitic, antitumor, antiviral, antibacterial, anti-inflammatory, anti-SARS-CoV-2 and neuropharmacological activities. Therefore, aurones have attracted the attention of more and more chemists and pharmaceutical chemists, who realized that it is possible to get lead compounds with better activities <i>via</i> structural modifications of aurones. In some research works, aurone and its derivatives have exhibited good activity, e.g., Xie discovered the heterocyclic variant of the (Z)-2-benzylidene-6-hydroxybenzofuran-3(2H)- one scaffold that possessed low nanomolar <i>in vitro</i> potency in cell proliferation assays using various cancer cell lines, <i>in vivo</i> potency in prostate cancer PC-3 xenograft and zebrafish models, and absence of appreciable toxicity, which proved that aurones are valuable compounds worthy of further study. Herein, the biological activities of aurone derivatives are reviewed, which covers the literature since 2000, in which the strategies to develop bioactive aurone derivatives and the structureactivity relationship are highlighted.

Millepora “fire coral” toxins: An overview of their biological activities

Millepora “fire coral” toxins: An overview of their biological activities

Green and Sustainable Extraction of Bioactive Compounds from Centella asiatica leaves using Microwave Pretreatment and Ultrasonication: Kinetics, Process Optimization, and Biological Activity

Green and Sustainable Extraction of Bioactive Compounds from Centella asiatica leaves using Microwave Pretreatment and Ultrasonication: Kinetics, Process Optimization, and Biological Activity

Development of novel imidazole-2-one/-2-thione heterocyclic derivatives as antimicrobial and antiproliferative agents and evaluation through biological studies and molecular modeling simulations

Development of novel imidazole-2-one/-2-thione heterocyclic derivatives as antimicrobial and antiproliferative agents and evaluation through biological studies and molecular modeling simulations

Structural and Biological Evaluation of Manganese(II) and Copper(II) Complexes with 2,5-Pyridinedicarboxylate Ligands: Experimental and Theoretical Insights into Antibacterial Potential and Antibiofilm Property

Structural and Biological Evaluation of Manganese(II) and Copper(II) Complexes with 2,5-Pyridinedicarboxylate Ligands: Experimental and Theoretical Insights into Antibacterial Potential and Antibiofilm Property

Potential application of new antimicrobials biological macromolecule of CNT/ZnO-NPs based on hydroxypropyl methylcellulose/shellac as packaging films.

Potential application of new antimicrobials biological macromolecule of CNT/ZnO-NPs based on hydroxypropyl methylcellulose/shellac as packaging films.

Preparation of agar polysaccharides and biological activities and relationships of agar-derived oligosaccharides and monosaccharides: A review.

Preparation of agar polysaccharides and biological activities and relationships of agar-derived oligosaccharides and monosaccharides: A review.

Polyethyleneimine-modified Laminarin nanoparticles as a novel vaccine adjuvant for ovalbumin to enhance the immune responses.

Polyethyleneimine-modified Laminarin nanoparticles as a novel vaccine adjuvant for ovalbumin to enhance the immune responses.

Biochemical characterization of the catalytic domain from a novel hyperthermophilic β-glucanase and its application for KOS production.

Biochemical characterization of the catalytic domain from a novel hyperthermophilic β-glucanase and its application for KOS production.

Extraction methods, structural features and bioactivity diversity of polysaccharides from the genus Chrysanthemum: A review.

Extraction methods, structural features and bioactivity diversity of polysaccharides from the genus Chrysanthemum: A review.