Recovery Of Biodiversity Research Articles

Ina

Indonesian government has enacted regulations requiring reclamation and rehabilitation activities on post coal mining areas. However, the evaluation related to the ecological restoration concepts is yet unavailable. This study aims to evaluate the regulations on rehabilitation and reclamation of post coal mining areas in Indonesia conforming on ecological restoration concepts. Employing desk study and content anaysis, this research focussed on three technical regulations concerning reclamation guidance and post-mining evaluation guidance, namely ESDM Decree No 7 Year 2014, MoF Decree No P.60/Menhut-II/2009, and MoF Decree No P.4/Menhut-II/2011. Each regulation was comprehensively evaluated whether the regulation already regulates matters relating to ecological restoration criterias, such as biodiversity recovery, soil remediation, planting of local plants, bans on invasive plant species and monitoring and evaluation. The result showed that there was no regulation complying with all ecological restoration criterias. Meanwhile, all the regulations have mandated monitoring and evaluation after rehabilitation and reclamation activities. However, there was no regulation mandating prohibition on planting invasive plant species. In conclusion, regulations related to post coal mining rehabilitation and reclamation have not fully complied with the ecological restoration concepts, therefore improvement to that regulations are necessary.

Biodiversity recovery following delta-wide measures for flood risk reduction.

Biodiversity declined markedly over the past 150 years, with the biodiversity loss in fluvial ecosystems exceeding the global average. River restoration now aims at flood safety while enhancing biodiversity and has had success locally. However, at the scale of large river distributaries, the recovery remained elusive. We quantify changes in biodiversity of protected and endangered species over 15 years of river restoration in the embanked floodplains of an entire river delta. We distinguish seven taxonomic groups and four functional groups in more than 2 million field observations of species presence. Of all 179 fluvial floodplain sections examined, 137 showed an increase in biodiversity, particularly for fast-spreading species. Birds and mammals showed the largest increase, that is, +13 and +3 percentage point saturation of their potential based on habitat. This shows that flood risk interventions were successfully combined with enhancement of biodiversity, whereas flood stage decreased (-24 cm).

Plant conservation in Australia: Current directions and future challenges

Australia is a large, old and flat island continent that became isolated following the breakup of the Gondwanan super continent. After more than 40–50 M years of independent evolution, approx. 600,000–700,000 species now call Australia home. More than 21,000 of these species are plants, with at least 84% of these being endemic. Plant taxa are protected, conserved and managed under a range of legislation at the State- and Territory-level as well as Federally for matters of national significance. This can create issues of misalignment among threatened species lists but generally there is co-operation among conservation agencies to reduce misalignments and to manage species irrespective of jurisdictional borders. Despite significant investment in programs designed to assist the recovery of Australian biodiversity, threatened plants in particular appear to be continuing to decline. This can be attributed to a range of factors including major threatening processes associated with habitat loss and invasive species, lack of public awareness of the cultural and socio-economic value of plant conservation, and our relatively poor understanding of basic species taxonomy and biology, especially for those species that have specific interactions with pollinators, symbionts and herbivores. A recent shift in Federally-based conservation programs has been to identify 30 key plant species for recovery through the setting of measurable targets, improving the support provided to recovery teams and encouraging industry, business and philanthropy to support conservation actions.

Can Ecological Restoration Meet the Twin Challenges of Global Change and Scaling Up, Without Losing Its Unique Promise and Core Values?

The words we use to describe phenomena in science shape our understanding of those phenomena, much more so than we often realize. This is especially true in fields driven by strong policy agendas, like restoration ecology and the practice of ecological restoration. The twin challenges of accelerating global change and upscaling global restoration practice make it more imperative than ever to define the terms and the scope of ecological restoration clearly, and differentiate it from other ameliorative land management practices like rehabilitation. Poor definitions and loose use of language will otherwise lead to muddled conception and planning of projects, confused and disappointed stakeholders, and failure to exploit the enormous potential of this radical conservation strategy for both human well-being and the recovery of biodiversity and ecosystem functions. It is also important to be aware of the rhetorical devices that have given some momentum to the so-called “novel” ecosystems concept within the restoration community. The advocates of this concept initially used it to alert restorationists to the gravity of the global change challenge. But it has been unfortunately formulated through increasingly polemical language to effect a major and dangerous policy shift: abandoning the pursuit of the ambitious but still valid promise inherent in the phrase “ecological restoration,” in favor of the management of degraded landscapes for diminished ecosystem goods and services. While we are always “restoring the future,” there is no good reason to abandon the goal of restoring ecosystems to their historical trajectories, and the historical reference system remains an essential tool for the identification of the specifics of this goal. The author considers the contributions to the symposium from these perspectives. He concludes that if we clarify the language we use about restoration, and are appropriately mindful of the dynamics of global change and the complex social and ecological dynamics of large-scale restoration, this discipline and practice can indeed mature to become the gold standard and cutting edge for conservation in this century.

Drivers of community assembly in tropical forest restoration sites: role of local environment, landscape, and space.

There is increasing recognition that community assembly theory can offer valuable insights for ecological restoration. We studied community assembly processes following tropical forest restoration efforts, using dung beetles (Scarabaeinae) as a focal taxon to investigate taxonomic and functional patterns of biodiversity recovery. We evaluated the relative importance of the local environment (i.e., canopy cover, understory cover, tree basal area, and soil texture), landscape context (i.e., habitat patch proximity and availability and percentage of surrounding area classified as natural forest or Eucalyptus spp. plantation), and space (i.e., spatial proximity of the study areas to estimate dispersal limitation or unmeasured spatially structured processes) on dung beetle species and functional trait composition across a gradient of 15 restoration sites in Brazilian Atlantic Forest. We also assessed which factors were the primary determinants in the establishment of individual dung beetle functional groups, classified according to size, food relocation habit, diet, and period of flight activity. Both species and functional trait composition were most strongly influenced by the local environment, indicating that assembly was predominantly driven by niche-based processes. Most of the variation explained by space was co-explained by local environment and landscape context, ruling out a strong influence of dispersal limitation and random colonization on assembly following restoration. In addition, nearly all of the variance explained by landscape context was co-explained by local environment, suggesting that arrival and establishment at a site depends on both local and landscape-scale environmental factors. Despite strong evidence for niche-based assembly, a large amount of variation remained unexplained in all models, suggesting that stochastic processes and/or unmeasured environmental variables also play an important role. The relative importance of local environment, landscape context, and space changed considerably when analyzing the assembly mechanisms of each functional group separately. Therefore, to recover distinct functional traits in restoration sites, it may be necessary to manipulate different components of the local environment and surrounding landscape. Overall, this study shows that assembly rules can help to better understand recovery processes, enabling improvement of future restoration efforts.

Reconciliation ecology in practice: Legal and policy considerations when implementing temporary nature on undeveloped lands in the European Union

Strict regulations, such as the EU Nature Directives, remain pivotal for halting the downward spiral for some protected species. In recent years, though, it has become clear that nature protection rules, are also generating perverse incentives, especially when rigidly applied to areas that have already been transformed by human use, such as agricultural land, quarries and port sites. With the arrival of novel incentive concepts, such as temporary nature in several EU Member States, an unprecedented window of opportunity exists to reframe current nature protection rules. Temporary nature fosters private landowners, ranchers and project developers to actively participate in the recovery of endangered species, also in urban and industrial environments. In return for allowing nature to develop on their undeveloped and vacant lands, the project developers are provided with the legal guarantee that they can still subsequently develop their lands at a later stage. These newly founded conservation policies, which are increasingly endorsed by stand out as striking illustrations of the recently emerged branch of reconciliation ecology, since they aim at increasing biodiversity by opting for win-win scenarios in human-dominated landscapes. It is concluded that a more reconciliatory approach towards nature conservation, which goes beyond the ambit of protected areas, can serve as a catalyst for biodiversity recovery across the wider landscape. Further research will need to underpin whether the ambitious presumptions with regard to these well-intentioned and innovative approaches to nature conservation are justified.

Biodiversity and Ecosystem Functional Enhancement by Forest Restoration: A Meta‐analysis in China

AbstractLarge‐scale forest loss and degradation have dire consequences for biodiversity maintenance and provision of vital ecosystem services. Despite recent increasing efforts for forest restoration and sustainable management, there have been no comparative studies of biological taxonomy and multiple ecosystem functions to assess the effectiveness of forest restoration programmes, and how they vary through space and time. Here, we provided a quantitative assessment of the recovery of biodiversity and ecosystem functions by forest restoration in China using a meta‐analysis of 172 studies. We found that biodiversity and ecosystem functions were substantially increased in restored forests comparing with the degraded states. However, these restoration effects varied considerably by degradation origin, restoration approach, restoration age, ecological domains, taxonomic group and ecosystem function that is measured. Results also revealed that forest restoration from degraded states could not lead to full recovery of biodiversity and ecosystem functions, highlighting the irreplaceability of primary forests. We advocate allowing for natural or passive recovery, especially where biophysical conditions are favourable for spontaneous succession, or too harsh for human‐aided restoration, and choosing a combination of passive and active restoration measures based on adaptive management strategies. Our meta‐analysis provided fundamental insights into bridging the gap between small‐scale experiments and broad‐scale management needs towards highly effective and sustainable forest ecosystem restoration. Copyright © 2017 John Wiley & Sons, Ltd.

Revegetation rewilds the soil bacterial microbiome of an old field.

Ecological restoration is a globally important and well-financed management intervention used to combat biodiversity declines and land degradation. Most restoration aims to increase biodiversity towards a reference state, but there are concerns that intended outcomes are not reached due to unsuccessful interventions and land-use legacy issues. Monitoring biodiversity recovery is essential to measure success; however, most projects remain insufficiently monitored. Current field-based methods are hard to standardize and are limited in their ability to assess important components of ecosystems, such as bacteria. High-throughput amplicon sequencing of environmental DNA (metabarcoding of eDNA) has been proposed as a cost-effective, scalable and uniform ecological monitoring solution, but its application in restoration remains largely untested. Here we show that metabarcoding of soil eDNA is effective at demonstrating the return of the native bacterial community in an old field following native plant revegetation. Bacterial composition shifted significantly after 8years of revegetation, where younger sites were more similar to cleared sites and older sites were more similar to remnant stands. Revegetation of the native plant community strongly impacted on the belowground bacterial community, despite the revegetated sites having a long and dramatically altered land-use history (i.e. >100years grazing). We demonstrate that metabarcoding of eDNA provides an effective way of monitoring changes in bacterial communities that would otherwise go unchecked with conventional monitoring of restoration projects. With further development, awareness of microbial diversity in restoration has significant scope for improving the efficacy of restoration interventions more broadly.

La réparation de la nature et quelques-uns de ses enjeux du point de vue de l'évaluation des atteintes écologiques

Laws n° 2008-757 on environmental liability and n° 2016-1087 on the recovery of biodiversity, nature and landscape affirm a principle of compensation for nature damages in order to provide an equivalent ecological status to the damage inflicted on nature. This approach excludes the compensation by monetary equivalent. It is based on specific methods developed in recent years. Despite these valuable tools for the effectiveness of the French legal framework for environmental protection, strict ecological equivalence remains a difficult objective to be achieved in practice. It is important to be careful in the implementation of the ecological compensation.

Forest ecosystem-service transitions: the ecological dimensions of the forest transition

Wilson, S. J., J. Schelhas, R. Grau, A. S. Nanni, and S. Sloan. 2017. Forest ecosystem-service transitions: the ecological dimensions of the forest transition. Ecology and Society 22(4):38. https://doi.org/10.5751/ES-09615-220438

Above-Ground Carbon Sequestration during Restoration of Upland Evergreen Forest in Northern Thailand

Tropical deforestation reduces the global terrestrial carbon sink and substantially contributes towards global climate change. Conversely, tropical forest restoration could help to mitigate the problem, but few measurements of how much carbon can be absorbed by forest restoration have been published. Therefore, this study used a partial harvesting method to compare carbon sequestration among 11 framework tree species (selected to accelerate forest regeneration by suppressing weeds and attracting seed dispersers), in a restoration trial in northern Thailand. The goal was to enable restoration practitioners to factor in carbon sequestration, when selecting tree species to plant. Above-ground carbon sequestration was derived from wood density, tree volume and above-ground biomass of 3 trees of each of 12 tree species, in 5, 10 and 14-year old restoration plots (RF5, RF10 and RF14, respectively). Wood density did not vary significantly with tree age (p ≤ 0.05), but it did differ significantly among tree species (p ≤ 0.05). Gmelina arborea wood was the densest (0.57 ± 0.10 g/cm3). Carbon concentration of stem wood did not vary significantly among tree species or age (p ≤ 0.05), averaging 44.67% (±0.54). Tree volume varied among the species in the youngest plot, but such variation declined with tree age. In the oldest plot (RF14), Erythrina subumbrans and Spondias axillaris grew significantly larger than the other species and sequestered the most above-ground carbon: 135.23 and 115.87 kgC/tree respectively. Bischofia javanica sequestered the least, only 9.80 kgC/tree. An even framework species mix would sequester 13.2, 44.3 and 105.8 tC/ha, 5, 10 and 14 years respectively after planting and would achieve carbon storage levels similar to those of nearby natural forest in 16 - 17 years. The framework species method is therefore capable of rapidly accumulating carbon, a property which, along with its acceleration of biodiversity recovery and provision of a wide range of forest products and ecological services to local people, meets both the requirements and safeguards of REDD+ projects.

Recovery of floristic diversity and basal area in natural forest regeneration and planted plots in a Costa Rican wet forest

AbstractWhen compared to planted reforestation, natural unassisted regeneration is often reported to result in slow recovery of biomass and biodiversity, especially early in succession. In some cases, naturally regenerating forests are not comparable to the community structure of primary forests after many decades. However, direct comparison of the outcomes of tropical forest restoration and natural regeneration is hindered by differences in metrics of forest recovery, inconsistency in land use histories, and dissimilarities in experimental design. We present the results of a replicated reforestation experiment comparing natural regeneration and polyculture tree planting at multiple diversity levels (3, 6, 9, or 12 native tree species), with uniform land use history and initial edaphic conditions. We compare the recovery of basal area and floristic diversity in these treatments after 5 yr of succession. Total basal area was higher in planted plots than in naturally regenerating plots, but it but did not vary among the different planted diversity levels. The basal area of woody recruits did not differ among treatments. The diversity of woody recruits increased substantially over time but did not vary among planting treatments. Species composition trajectories showed directional turnover over time, with no consistent differences among treatments. The convergence of restoration trajectories and similarity of floristic community diversity and composition across all treatments, after only 5 yr, provides evidence of the viability of natural regeneration for rapid restoration of forest biodiversity.

Natural regeneration and biodiversity: a global meta‐analysis and implications for spatial planning

AbstractNatural regeneration offers a cheaper alternative to active reforestation and has the potential to become the predominant way of restoring degraded tropical landscapes at large‐scale. We conducted a meta‐analysis for tropical regions and quantified the relationships between both ecological and socioeconomic factors and biodiversity responses in naturally regenerating areas. Biogeographic realms, past disturbance, and the human development index (HDI) were used as explanatory variables for biodiversity responses. In addition, we present a case study of large‐scale natural regeneration in the Brazilian Atlantic Forest and identify areas where different forms of restoration would be most suitable. Using our dataset for tropical regions, we showed that natural regeneration was predominantly reported within: the Neotropical realm; areas that were intensively disturbed; and countries with medium HDI. We also found that biodiversity in regenerating forests was more similar to the values found in old growth forests in: countries with either low, high, or very high HDI; less biodiverse realms; and areas of less intensive past disturbance. Our case study from Brazil showed that the level of forest gain resulting from environmental legislation, in particular the Brazilian Forest Code, has been reduced, but remains substantial. Complementary market incentives and financial mechanisms to promote large‐scale natural regeneration in human‐modified agricultural landscapes are also needed. Our analysis provides insights into the factors that promote or limit the recovery of biodiversity in naturally regenerating areas, and aids to identify areas with higher potential for natural regeneration.

Does regional diversity recover after disturbance? A field experiment in constructed ponds.

The effects of disturbance on local species diversity have been well documented, but less recognized is the possibility that disturbances can alter diversity at regional spatial scales. Since regional diversity can dictate which species are available for recolonization of degraded sites, the loss of diversity at regional scales may impede the recovery of biodiversity following a disturbance. To examine this we used a chemical disturbance of rotenone, a piscicide commonly used for fish removal in aquatic habitats, on small fishless freshwater ponds. We focused on the non-target effects of rotenone on aquatic invertebrates with the goal of assessing biodiversity loss and recovery at both local (within-pond) and regional (across ponds) spatial scales. We found that rotenone caused significant, large, but short-term losses of species at both local and regional spatial scales. Using a null model of random extinction, we determined that species were selectively removed from communities relative to what would be expected if species loss occurred randomly. Despite this selective loss of biodiversity, species diversity at both local and regional spatial scales recovered to reference levels one year after the addition of rotenone. The rapid recovery of local and regional diversity in this study was surprising considering the large loss of regional species diversity, however many aquatic invertebrates disperse readily or have resting stages that may persist through disturbances. We emphasize the importance of considering spatial scale when quantifying the impacts of a disturbance on an ecosystem, as well as considering how regional species loss can influence recovery from disturbance.

Forest regeneration affects dung beetle assemblages (Coleoptera: Scarabaeinae) in the southern Brazilian Atlantic Forest

The Brazilian Atlantic Forest is one of the most diverse environments, but it is also one of the most threatened areas in terms of loss of biodiversity and ecosystem services. Assessment of changes in the community structure during the recovery of forests can be performed using indicator organisms. Dung beetles perform several ecological functions and show high sensitivity to natural and anthropogenic environmental changes. This study aimed to investigate the effect of regeneration time of Atlantic Forest sites on structure of Scarabaeinae assemblages. We sampled dung beetles using ten baited pitfall traps per site, in six sites grouped into three classes of forest regeneration time (~30, ~60 and >80 years) in the southern Brazilian Atlantic Forest, during January 2015. A total of 520 individuals belonging to 16 species and nine genera of dung beetles were sampled. Rarefied species richness did not differ between sites with different regeneration times. Average species richness and abundance of Scarabaeinae was smaller in areas of shorter recovery time. True alpha diversity was higher in areas with intermediate recovery whereas Shannon diversity showed higher values in areas of shorter recovery. Approximately 29 % of the variation in abundance data of Scarabaeinae was explained by environmental variables, with one-third of this variation explained also by spatial predictors. External factors such as landscape management and farming practices in the surroundings must be taken into consideration in management plans and the management of natural areas for the recovery of biodiversity in the Atlantic Forest. These external factors can considerably affect the structure of communities and lead to scenarios of greater diversity in intermediate regeneration sites due to the heterogeneity of the landscape.

Ten-year responses of ground-dwelling spiders to retention harvest in the boreal forest.

The Ecosystem Management Emulating Natural Disturbances (EMEND) project tests the hypothesis that varying levels of green tree retention maintain and retain forest biodiversity better than conventional clear-cutting. We studied epigaeic spiders to assess biodiversity changes 2, 5, and 10yr following a range of partial retention harvests (clear-cut, 10-75% retention) and unharvested controls in four boreal mixedwood cover types. A total of 56 371 adult spiders representing 220 species was collected using pitfall traps. Lasting effects on forest structure were proportional to harvest intensity. These changes strongly influenced spider richness, abundance, and species composition, as well as assemblage recovery. Distinctive assemblages were associated with disturbance level, especially with partial harvests (≤50% retention), and these were dominated by open-habitat species even 10yr after harvest. Assemblages were more similar to those of controls in the highest (75%) retention treatment, but significant recovery toward the structure of pre-disturbance assemblages was not detected for any prescription in any cover type. Although early responses to retention harvest suggested positive effects on spider assemblages, these are better explained as lag effects after harvest because assemblages were less similar to those of unharvested controls 5yr post-harvest, and only minor recovery was observed 10yr following harvest. Retention of forest biodiversity decreased over time, especially in conifer stands and the lower (10-50%) retention treatments. Overall, retention harvests retained biodiversity and promoted landscape heterogeneity somewhat better than clear-cutting; however, there was a clear gradient of response and no retention "threshold" for conservation can be recommended on the basis of our data. Furthermore, results suggest that retention harvest prescriptions should be adjusted for cover type. We show that low retention ameliorated impacts in broadleaved forests characteristic of earlier stages in mixedwood succession, but only higher retention was associated with less impact in successionally older conifer forests. Although these short-term responses (10yr) of spider assemblages support use of retention harvests, understanding the true conservation merit of these practices, relative to conventional approaches, requires evaluation over longer time scales, with work more focused on recovery of biodiversity than on its preservation after harvest.

Eradicating the grey squirrel Sciurus carolinensis from urban areas: an innovative decision-making approach based on lessons learnt in Italy.

Eradication of invasive alien species supports the recovery of native biodiversity. A new European Union Regulation introduces obligations to eradicate the most harmful invasive species. However, eradications of charismatic mammals may encounter strong opposition. Considering the case study of the eastern grey squirrel (Sciurus carolinensis Gmelin, 1788) in central Italy, we developed a structured decision-making technique based on a Bayesian decision network model and explicitly considering the plurality of environmental values of invasive species management to reduce potential social conflicts. The model identified priority areas for management activities. These areas corresponded to the core of the grey squirrel range, but they also included peripheral zones, where rapid eradication is fundamental to prevent the spread of squirrels. However, when the model was expanded to integrate the attitude of citizens towards the project, the intervention strategy slightly changed. In some areas, the citizens' support was limited, and this resulted in a reduced overall utility of intervention. The suggested approach extends the scientific basis for management decisions, evaluated in terms of technical efficiency, feasibility and social impact. Here, the Bayesian decision network model analysed the potential technical and social consequences of management actions, and it responded to the need for transparency in the decision-making process, but it can easily be extended to consider further issues that are common in many mammal eradication programmes. Owing to its flexibility and comprehensiveness, it provides an innovative example of how to plan rapid eradication or control activities, as required by the new EU Regulation. © 2016 Society of Chemical Industry.

Expanding marine protected areas to include degraded coral reefs.

Marine protected areas (MPAs) are a commonly applied solution to coral reef degradation, yet coral reefs continue to decline worldwide. We argue that expanding the range of MPAs to include degraded reefs (DR-MPA) could help reverse this trend. This approach requires new ecological criteria for MPA design, siting, and management. Rather than focusing solely on preserving healthy reefs, our approach focuses on the potential for biodiversity recovery and renewal of ecosystem services. The new criteria would help identify sites with the highest potential for recovery and the greatest resistance to future threats (e.g., increased temperature and acidification) and sites that contribute to MPA connectivity. The DR-MPA approach is a compliment rather than a substitute for traditional MPA design approaches. We believe that the DR-MPA approach can enhance the natural, or restoration-assisted, recovery of DRs and their ecosystem services; increase total reef area available for protection; promote more resilient and better-connected MPA networks; and improve conditions for human communities dependent on MPA ecosystem services.

Quantifying the impacts of grassland restoration on biodiversity and ecosystem services in China: A meta-analysis

Given the extent of global ecosystem degradation resulting from environmental changes and human activity, restoration efforts have increasingly focused on biodiversity and ecosystem services. Grassland, the largest terrestrial ecosystem globally and in China, has high ecological and economic value. We performed a meta-analysis to assess the impacts of grassland restoration on biodiversity and ecosystem services in China. The results showed that grassland restoration improved biodiversity by 32.44% and ecosystem services by 30.43%, although the restored grassland from degraded conditions failed to reach the level of non-degraded reference conditions for biodiversity and ecosystem services. The analysis of biodiversity and ecosystem services in the four ecological domains showed significant differences in restored grassland compared to the degraded and reference grassland. Restoration outcomes of biodiversity and ecosystem services were affected by different restoration approaches, but restoration age was not detected as significantly correlated with biodiversity and ecosystem services recovery. Biodiversity recovery, however, was positively correlated with ecosystem services recovery so far in our dataset. Despite this, these patterns require further elucidation and synthetic analyses must be conducted to assess and inform future restoration actions.

Soil Mesofauna Response to Cover Crops and Mycorrhizal Inoculated Plantation on Limestone Quarry Revegetation

Cover crops establishment followed by mycorrhizal inoculated tree plantation accelerate limestone quaryy restoration. Soil mesofauna is a useful indicator for assessing biodiversity recovery, as they are sensitive to human disturbance and involved in ecological processes. In this research, soil mesofauna presence used to evaluate limestone quarry restoration by cover crops and mycorrhizal inoculated tree esatblisment. Soil mesofauna are measured using individual density, species diversity, richness and important value. The study was conducted in four types of areas on limestone postmining lands are open areas/natural conditions without planting, cover crop area, non mycorrhizalinoculated plant area and mycorrhizal-inoculated plant area. The result showed The higher individual density, diversity and species richness of soil mesofauna on cover crops and mycorrhizal inoculated plant area than other areas are indicators of limestone quarry restoration rate. Furthermore the presence of Formicidae family that dominated on cover crops and mycorrhizal inoculated plant area, and emergence of Acari group only on mycorrhizal inoculated area also an indicator of limestone quarry restoration.