Platelet Biochemistry Research Articles

Source of hydrogen peroxide and of chemiluminescence observed in activated human platelet preparations

Human platelet suspensions can be observed to produce small amounts of H2O2 (0.04 nmoles H2O2/min/2.5 X 10(5) cells/cu mm) and measurable chemiluminescence when exposed to target particles for phagocytosis, such as latex spherules. Both H2O2 production and chemiluminescence are characteristic of phagocytosing polymorphonuclear leukocytes (PMN) and analysis of the purified platelets indicates contamination by PMN at the level of 0.2%. The amount of H2O2 produced and the chemiluminescence observed can be duplicated by adding latex spheres to a preparation of PMN at a concentration equivalent to the contaminant in the platelet preparations. We conclude that the H2O2 produced and chemiluminescence observed from activated platelets is due to the presence of small amounts of contaminating PMN. These studies emphasize the importance of controlling for PMN contamination in studies of platelet biochemistry and physiology.

Source of Hydrogen Peroxide and of Chemiluminescence Observed in Activated Human Platelet Preparations

Human platelet suspensions can be observed to produce small amounts of H2O2 (0.04 nmoles H2O2/min/2.5 X 10(5) cells/cu mm) and measurable chemiluminescence when exposed to target particles for phagocytosis, such as latex spherules. Both H2O2 production and chemiluminescence are characteristic of phagocytosing polymorphonuclear leukocytes (PMN) and analysis of the purified platelets indicates contamination by PMN at the level of 0.2%. The amount of H2O2 produced and the chemiluminescence observed can be duplicated by adding latex spheres to a preparation of PMN at a concentration equivalent to the contaminant in the platelet preparations. We conclude that the H2O2 produced and chemiluminescence observed from activated platelets is due to the presence of small amounts of contaminating PMN. These studies emphasize the importance of controlling for PMN contamination in studies of platelet biochemistry and physiology.

Book Reviews

Book reviewed in this article Human Blood Coagulation, Haemostasis and Thrombosis, 2nd edition. Edited by R. BIGGS. Biochemistry and Pharmacology of Platelets. Ciba Foundation Symposium 35 (new series) Edited by KATHERINE ELLIOTT and JULIE KNIGHT. Congenital Disorders of Erythropoiesis. Ciba Foundation Symposium (New series). Haemostasis in Acute Neurological Disorders. By CH. J. VECHT. Van Gorcum, Amsterdam, 1975.

Platelet Biochemistry. A unique teaching/learning situation

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPlatelet Biochemistry. A unique teaching/learning situationBarth H. Ragatz Cite this: J. Chem. Educ. 1974, 51, 11, 724Publication Date (Print):November 1, 1974Publication History Received3 August 2009Published online1 November 1974Published inissue 1 November 1974https://doi.org/10.1021/ed051p724RIGHTS & PERMISSIONSArticle Views54Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (712 KB) Get e-Alerts Get e-Alerts

Regulation of Hematopoiesis, vol 1, Red Cell Production; vol 2, White Cell and Platelet Production

ABSTRACT From time to time a book will be published which is widely accepted as the bible in a particular field of endeavor. Wintrobe's Clinical Hematology has enjoyed such status among clinical hematologists for some years. I believe the present set deserves a parallel position with the research hematologist.This is a scholarly review and discussion of modern research techniques that have helped elucidate the development, fine structure, biochemistry, function, kinetics, and control of erythrocytes, neutrophils, eosinophils, monocytes, lymphocytes, and platelets. There are still many unanswered questions. The inquisitive mind can find in these volumes numerous ideas to pursue and the tools for such pursuit. Sixtythree leading investigators contribute 58 chapters under ten general subject headings. There is some redundancy, as one might expect with multiple contributors. A number of pertinent charts, tables, and photomicrographs illustrate these topics. Each chapter has an extensive, pertinent, and contemporary bibliography. In a final chapter

Biochemistry of blood platelets. Interaction of activated factor X with platelets

Biochemistry of blood platelets. Interaction of activated factor X with platelets

Biochemistry of Blood Platelets. Colloquim Held on Occasion of the Third Meeting of the Federation of European Biochemical Societies, Organized by the Polish Biochemical Society, Warszawa, April 4th-7th, 1966.E. Kowalski , S. Niewiarowski

<i>Biochemistry of Blood Platelets. Colloquim Held on Occasion of the Third Meeting of the Federation of European Biochemical Societies, Organized by the Polish Biochemical Society, Warszawa, April 4th-7th, 1966.</i>E. Kowalski , S. Niewiarowski

Biochemistry of Platelets

Biochemistry of Blood Platelets Edited by E. Kowalski and S. Niewiarowski. (Colloquium held on the Occasion of the Third Meeting of the Federation of European Biochemical Societies, Warsaw, April 4–7, 1966.) Pp. vii + 191. (London: Academic Press, Inc. (London), Ltd.; New York: Academic Press, Inc.; Warsaw: PWN–Polish Scientific Publishers, 1967.) 45s.; $8.