Biochemical Responses Of Plants Research Articles

Response of Antioxidant Enzymes of Winter Wheat (Triticum aestivum L.) to Shallow and Saline Groundwater Depths

ABSTRACTAntioxidant enzymes in plants are critical for protection against oxidative stress and for the overall health and resilience of plant systems. However, antioxidant responses of plants grown in shallow and saline groundwater are poorly understood. Therefore, understanding the biochemical responses of plants to shallow groundwater significantly affects food security and environmental conservation. With this aim, the present work was carried out for 2 years in drainable lysimeters to assess the effects of four different groundwater salinities (0.38, 2.0, 4.0 and 8.0 dS m−1) on the temporal changes in antioxidant enzymatic activity in wheat plants under three different groundwater depths (30, 55 and 80 cm). Superoxide dismutase (SOD), glutathione S‐transferase (GST) and catalase (CAT) activities varied significantly based on groundwater depth and salinity. CAT and GST enzyme levels increased curvilinearly with rising groundwater depth and salinity. Conversely, the GR enzyme activity showed no significant change with groundwater depths but increased linearly with higher salinity. SOD enzyme activity notably increased at a groundwater depth of 30 cm but decreased at a depth of 80 cm. Moreover, the peak activity of the GR enzyme was observed at a 6 dS m−1 groundwater salinity under groundwater depths. Additionally, the GST and CAT enzyme activities were inhibited more when the groundwater depth was <55 cm and the groundwater salinity was >4.20 dS m−1. Finally, identifying the peak levels of antioxidant enzymes could potentially serve as an indicator for determining the optimal timing for applying stress mitigation methods in areas with shallow and saline groundwater.

Recent omics progress in nanobiotechnology for plant abiotic stress tolerance improvement

Use of nanomaterials (NMs) to improve plant abiotic stress tolerance (AST) is a hot topic in NM-enabled agriculture. Previous studies mainly focused on the physiological and biochemical responses of plants treated with NMs under abiotic stress. To use NMs for improving plant AST, it is necessary to understand how they act on this tolerance at the omics and epigenetics levels. In this review, we summarized the knowledge of NM-improved abiotic stress tolerance in relation to omics (such as metabolic, transcriptomic, proteomic, and microRNA), DNA methylation, and histone modifications. Overall, NMs can improve plant abiotic stress tolerance through the modulation at omics and epigenetics levels.

Phytofabricated gold nanoparticles as modulators of salt stress responses in spinach: implications for redox homeostasis, biochemical and physiological adaptation.

The utilization of plant material for synthesizing nanoparticles effectively triggers physiological and biochemical responses in plants to combat abiotic stresses. Salt stress, particularly caused by NaCl, significantly affects plant morphology and physiology, leading to reduced crop yields. Understanding the mechanisms of salt tolerance is crucial for maintaining crop productivity. In this study, we examined the effects of 150 μM spinach-assisted gold nanoparticles (S-AuNPs) on various parameters related to seed germination, growth attributes, photosynthetic pigments, stomatal traits, ion concentrations, stress markers, antioxidants, metabolites, and nutritional contents of spinach plants irrigated with 50 mM NaCl. Results showed that S-AuNPs enhanced chlorophyll levels, leading to improved light absorption, increased photosynthates production, higher sugar content, and stimulated plant growth under NaCl stress. Stomatal traits were improved, and partially closed stomata were reopened with S-AuNPs treatment, possibly due to K+/Na+ modulation, resulting in enhanced relative water content and stomatal conductance. ABA content decreased under S-AuNPs application, possibly due to K+ ion accumulation. S-AuNPs supplementation increased proline and flavonoid contents while reducing ROS accumulation and lipid peroxidation via activation of both non-enzymatic and enzymatic antioxidants. S-AuNPs also regulated the ionic ratio of K+/Na+, leading to decreased Na+ accumulation and increased levels of essential ions in spinach plants under NaCl irrigation. Overall, these findings suggest that S-AuNPs significantly contributeto salt stress endurance in spinach plants by modulating various physiological attributes.

Silver nanoparticles as potential fungicide against rice brown spot: physiological and biochemical responses in plants

Silver nanoparticles as potential fungicide against rice brown spot: physiological and biochemical responses in plants

Potentially Toxic Metals: Their Effects on the Soil-Human Health Continuum

As the foundation of nutritious foods lies in the soil, the consumption of crops grown in contaminated soils may pose an elevated risk of health issues through the soil-plant-human pathway. The impact of heavy metals (HMs) and metalloids on the physiological and biochemical responses of plants can have adverse effects on both growth and yield. The excessive accumulation of these substances in plant tissues poses a significant challenge to public health. HMs possess the capability not only to function as carcinogens but also to act as co-carcinogens, thereby activating specific chemical compounds. According to the World Health Organization (WHO) reports, the target values, representing the desirable maximum concentrations of HMs in the soil, follow the order: Cr>Pb>Zn>Cu>Ni>Cd. This implies that Cd poses the highest potential risk, given its target value of 0.8 mg/kg, while Cr carries the lowest potential risk, with a target value of 100 mg/kg. Various agricultural management practices are recognized as significant pathways that induce the accumulation of metals in the soil and the surrounding environment. Hence, understanding the origin and status of HMs in the environment, along with assessing their potential risks and developing strategies to mitigate these risks, becomes crucial. This study aims to evaluate different facets of the soil-human health continuum concerning potentially toxic metals.

Biochemical responses of Echinochloa polystachya inoculated with a Trichoderma consortium during the removal of a pyrethroid-based pesticide

The biochemical response of plants exposed to pesticides and inoculated with microorganisms is of great importance to explore cleaning up strategies for contaminated sites with pyrethroid-based pesticides. We evaluated the effects of a Trichoderma consortium on the biochemical responses of Echinochloa polystachya plants during the removal of a pyrethroid-based pesticide. Plants were inoculated or not with the Trichoderma consortium and exposed to commercial pesticide H24®, based on pyrethroids. Pesticide application resulted in significant reduction in root protein content (58%), but enhanced content of malondialdehyde (MDA) in shoots, superoxide dismutase (SOD) activity in shoots and roots, and catalase (CAT) activity in roots. Inoculation of Trichoderma consortium in E. polystachya exposed to the pesticide resulted in increased protein content in roots and MDA content in shoots (2-fold). Trichoderma consortium improved protein content and SOD activity (140-fold) in plants. Fungal inoculation increased the removal (97.9%) of the pesticide in comparison to the sole effect of plants (33.9%). Results allow further understanding about the responses of the interaction between plants and root-associated fungi to improving the assisted-phytoremediation of solid matrices contaminated with organic pesticides.

Silicon application enhances wheat defence against Sitobion avenae F. by regulating plant physiological-biochemical responses

Sitobion avenae F. is a highly prevalent and devastating pest in wheat crops, leading to significant yield losses. Silicon (Si) has been widely recognized as an effective inducer of plant resistance against aphids. Nevertheless, the underlying mechanisms governing the physiological and biochemical responses of plants induced by Si defense against S. avenae F. remain incompletely understood. In this study, we conducted experiments by treating wheat leaves with varying concentrations of Tetraethyl orthosilicate (TEOS) spray under aphid infestation. We meticulously observed and recorded the life cycle of S. avenae F. and measured the content of plant hormones, secondary metabolites, and the activity of defense enzymes in wheat leaves. Furthermore, we utilized structural equation modeling to discern the causal correlation between aphid performance and the physiological-biochemical responses of wheat under TEOS sprays. Our findings revealed that a concentration of 3 mmol/L TEOS significantly shortened the net reproductive rate, intrinsic rate and finite rate of increase, and mean generation time of S. avenae F., while simultaneously prolonging the population doubling time. Additionally, the content of lignin, total phenolics, flavonoids, alkaloids, tannin, jasmonic acid (JA), and salicylic acid in wheat leaves exhibited a substantial increase. Furthermore, the activity of phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO), catalase, and lipoxygenase in wheat leaves was significantly enhanced. Our results suggest that TEOS spray reduced the survival and population growth of S. avenae F. while enhancing the defense response of wheat against aphids by activating the activity of PAL and PPO in wheat, and increasing the content of total phenolic and JA. This work provides valuable insights for the development of appropriate Si fertilizers for effective pest management and offers robust theoretical support for wheat aphid control through agricultural fertilization strategies.

Mancozeb associated with water deficit: Physiological and biochemical responses of soybean plants

Can oxidative stress from xenobiotics be intensified under water deficit conditions? Based on the hypothesis that the exposure to a xenobiotic aggravates the oxidative damages induced by water deficit, soybean plants were cultivated in pots eqquipped with soil water potential sensors to continuously monitor soil moisture levels. The experiment was conducted in 2020, in a protected environment, using a randomized block design with three replications. To comprehensively evaluate physiological and biochemical responses of plants, we conducted a range of analyses, including measurements of stomatal conductance, leaf temperature, chlorophyll, chlorophyll fluorescence, relative water content, membrane damage, electrical conductivity, lipid peroxidation, concentration of reactive oxygen species – hydrogen peroxide and superoxide, activity of antioxidative enzymes – ascorbate peroxidase, superoxide dismutase, catalase, non-enzymatic compounds – non protein thiols, nitric oxide and proline. Plants that received the application of the fungicide mancozeb showed 1 °C higher temperature compared to the control plants. Irrigated soybean plants had higher nitric oxide content when treated with mancozeb, but under water deficit the higher content was observed in control plants. No deleterious effects of mancozeb as a xenobiotic were observed when applied at the recommended dose for soybean cultivation, even under water deficit conditions. The plants exhibited deleterious effects caused by water deficit, particularly in relation to oxidative stress induced by water shortage. The combination of two stresses (water deficit and xenobiotics) did not result in the maximization of negative effects caused by water stress. By refuting the working hypothesis, it becomes evident that the application of mancozeb to plants under water deficit conditions did not exacerbate the damage induced by this stress. Responses to this combination are contingent upon various factors, including the stress duration, its severity, the plant species involved, and, in the case of xenobiotics, their inherent properties and mode of action within the plant.

Low water availability increases susceptibility to peanut smut (Thecaphora frezzii) in peanut crop

AbstractPeanut smut (causal agentThecaphora frezzii) and seasonal drought are the two main factors reducing peanut yields in Argentina. There are no previous studies about the effect of drought on peanut smut occurrence. We evaluated the effect of soil water limitation on smut symptoms in greenhouse and field assays. Additionally, we analysed the biochemical responses of plants to the combined stress caused by water limitation and smut infection in greenhouse experiments. We found that a moderate water deficit (30% of soil water‐holding capacity) increased smut symptoms and differentially increased proline and reduced chlorophylls in the host. Subsequently, we studied the correlation between field precipitation data and smut damage from 2015 to 2020 in naturally infected fields with a highT.frezziispore load in the soils. Strong correlations between precipitation and severely affected pods, severity disease index and incidence were found from January to March (susceptibility window forT.frezziiinfections). We suggest a strategy of irrigation in a specific time frame to mitigate smut damage when there is a water deficit in the growing season.

Effect of NPK fertilizer on the biochemical response of tomatoes (Solanum lycopersicum L.)

Increasing nutrient inputs affect plants and soil. Long-term, repeated mineral fertilizer applications may alter the agro-ecosystem. The application of the right amount of fertilizer is primordial to maintaining and improving sustainable plant productivity. The physiological response of the plant provides meaningful information on yield and makes it possible to better choose the doses of fertilization amendments. In order to determine the adequate dose of NPK fertilizer under economically profitable and more environmentally sustainable conditions, we investigated the impact of the chemical fertilizer amended at different NPK fertilizer doses (15, 20, and 25 kg ha-1) on the growth parameters, oxidative metabolism, and yield of Solanum lycopersicum. A complete random block experimental set-up with three doses and four replicates was performed for a greenhouse tomato crop. The results showed that NPK fertilizer influences morphological parameters, and phenolic compounds with the best data correlated to the medium treatment. Likewise, the content of flavonoids, lycopene, and β-carotene in tomato fruits displays a similar trend of variation with all treatment doses. The biochemical responses of plants to mineral fertilizer indicate that a medium dose is suitable, without overuse of fertilizers and pesticides, to reduce the negative impact on the agro-ecosystem.

Morphological and Biochemical Responses of Vigna unguiculata ssp. sesquipedalis in Different Zinc Concentrations

Zinc is an essential trace element required by plants. However, high zinc concentrations can lead to environmental pollution and plant toxicity. This research aimed to investigate how plants respond to different concentrations of zinc (0, 100, 200 and 300 ppm) in soil, using Vigna unguiculata ssp. sesquipedalis (Yard long bean) as a model plant. A total of 12 parameters were collected including plant morphological characteristics such as plant height, leaf number, yield, root length, pod length and fresh weight. Furthermore, key biochemical properties including chlorophyll content, total protein content, total phenolic and flavonoid content in plants were analyzed, in addition to soil pH and electrical conductivity. These parameters were used to determine the morphological and biochemical responses of plants under zinc-stress conditions. The results indicated that different concentrations of zinc significantly decreased the leaf number and pod length V. unguiculata. Soil electrical conductivity was significantly high at 200 ppm zinc. Significant changes in total protein were observed in stems and pods. Moreover, the total phenolic content in leaves showed a significant increase with higher zinc concentrations, while the opposite trend was observed for total phenolic content in V. unguiculata pods. In summary, varying concentrations of zinc had a significant impact on various morphological and biochemical properties of V. unguiculata, exhibiting a distinct pattern specific to each organ. This suggests that V. unguiculata is responsive, adaptive and capable of tolerating abiotic stress induced by a broad range of zinc concentrations.

Specific Streptomyces strain enhances the growth, defensive mechanism, and fruit quality of cucumber by minimizing its fertilizer consumption

BackgroundThe required amounts of chemical fertilizers (NPK) are determined by plant yield, and product quality is given less consideration. The use of PGPRs is an environmentally friendly approach that, in addition to increasing yield, also improves fruit quality. This study examined the role of specific Streptomyces strains in aiding cucumber plants to 1) use fewer NPK fertilizers in the same quantity 2) improve the quality of cucumber fruit, and 3) promote growth and defense system.ResultsIn this study, the effect of 17 Streptomyces strains on the vegetative traits of cucumber seedlings of the Sultan cultivar was evaluated as the first test. Four strains of Streptomyces with the highest root and shoot dry weight were selected from the strains. This experiment was performed to determine the interaction effect of selected strains and different amounts of NPK on cucumber yield, quality, physiological and biochemical responses of plants. The first experiment’s results revealed that strains IC6, Y7, SS12, and SS14 increased significantly in all traits compared to the control, while the other strains dramatically improved several characteristics. Analysis of variance (ANOVA) revealed significant differences between the effect of strains, NPK concentrations, and their interactions on plant traits. The treatments containing 75% NPK + SS12, yielded the most fruit (40% more than the inoculated control). Antioxidant enzymes assay showed that SS12 substantially increased the activity of POX, PPO, and the expression of the genes related to these two enzymes. Hormone assay utilizing HPLC analysis revealed that various strains employ a specific mechanism to improve the immune system of plants.ConclusionsTreatment with strain SS12 led to the production of cucumbers with the highest quality by reducing the amount of nitrate, and soluble sugars and increasing the amount of antioxidants and firmness compared to other treatments. A specific Streptomyces strain could reduce 25% of NPK fertilizer during the vegetative and reproductive growth period. Moreover, this strain protected plants against possible pathogens and adverse environmental factors through the ISR and SAR systems.

Overviewing Drought and Heat Stress Amelioration—From Plant Responses to Microbe-Mediated Mitigation

Microbes (e.g., plant-growth-promoting rhizobacteria, arbuscular mycorrhizal fungi and endophytes) are the natural inhabitants of the soil-plant-environment ecosystem having the potential to ameliorate the negative effects of environmental extremities. Plant-microbe interactions are integral events of agricultural ecosystems which must be studied in order to modulate the systemic mechanisms in field crops. Under changing climatic scenarios, drought and heat stresses tend to induce numerous physiological, morphological, metabolic and biochemical alterations in crop plants, while microbes hold the potential to mitigate these adverse impacts in a sustainable way. However, plant-microbe interaction mechanisms remain understudied owing to their complexities in the rhizosphere and within the cellular systems of plants. In this review, we have attempted to summarize microbes’ interactions with crop plants that tend to influence hormonal and nutrients balance, and the biosynthesis of metabolites and phytohormones, etc. In particular, focus has been kept on the underlying mechanisms related to plant-microbe interactions which confer abiotic stress tolerance. Moreover, various physiological, morphological, metabolic and biochemical responses of plants subjected to water scarcity and elevated temperatures have been synthesized objectively. Lastly, from the perspective of microbes’ application as biofertilizers, both challenges and future research needs to develop microbe-mediated tolerance as a biologically potent strategy have been strategically pointed out.

A meta-analysis on morphological, physiologicaland biochemical responses of plants with PGPR inoculation under drought stress.

Plant growth-promoting rhizobacteria (PGPR) can help plants to resist drought stress. However, the mechanisms of how PGPR inoculation affect plant status under drought remain incompletely understood. We performed a meta-analysis of plant response to PGPR inoculation by compiling data from 57 PGPR-inoculation studies, including 2, 387 paired observations on morphological, physiological and biochemical parameters under drought and well-watered conditions. We compare the PGPR effect on plants performances among different groups of controls and treatments. Our results reveal that PGPR enables plants to restore themselves from drought-stressed to near a well-watered state, and that C4 plants recover better from drought stress than C3 plants. Furthermore, PGPR is more effective underdrought than well-watered conditions in increasing plant biomass, enhancing photosynthesis and inhibiting oxidant damage, and the responses of C4 plants to the PGPR effect was stronger than that of C3 plants under drought conditions. Additionally, PGPR belonging to different taxa and PGPR with different functional traits have varying degrees of drought-resistance effects on plants. These results are important to improve our understanding of the PGPR beneficial effects on enhanced drought-resistance of plants.

Melatonin Pretreatment Alleviated Inhibitory Effects of Drought Stress by Enhancing Anti-Oxidant Activities and Accumulation of Higher Proline and Plant Pigments and Improving Maize Productivity

Drought stress has been shown to have harmful effects on crop productivity worldwide, including in Pakistan, due to rapid climate change scenarios. Extensive work has been reported on the influential role of melatonin (MEL) in either foliar or seed-primed applications; however, its role in root application is seldom reported. We investigated plant biochemical responses, including anti-oxidants, plant pigments, leaf water characteristics, and maize crop production, with MEL treatment under mild and severe drought stress. Maize Cvar. Jalal was subjected to drought stress (60% and 80% of full irrigation) at the four-leaf stage, and MEL was applied as pretreatment with irrigation water at different doses (0, 100, and 200µM). The findings of the study revealed that the Chl a, b, and a + b contents and the carotenoid content significantly increased with MEL application during severe and mild drought stress. After applying 200 µM MEL, leaf water attributes, comprising relative water content (RWC), leaf water content (LWC), and relative saturation deficit (RSD), increased by 1.9%, 100%, and 71.2%, respectively, during mild drought and 17%, 133%, and 32% under severe drought. The anti-oxidant activities of POD, CAT, and APX were remarkably enhanced with MEL during drought stress. Our results showed that root application of 200 µM melatonin boosted seed yield and water productivity by 31% and 38%, and plant biomass increased by 32% and 29% under mild and severe drought stressors compared to plants with no MEL, leading to increased drought tolerance.

Light Spectral Composition Modifies Polyamine Metabolism in Young Wheat Plants.

Although light-emitting diode (LED) technology has extended the research on targeted photomorphogenic, physiological, and biochemical responses in plants, there is not enough direct information about how light affects polyamine metabolism. In this study, the effect of three spectral compositions (referred to by their most typical characteristic: blue, red, and the combination of blue and red [pink] lights) on polyamine metabolism was compared to those obtained under white light conditions at the same light intensity. Although light quality induced pronounced differences in plant morphology, pigment contents, and the expression of polyamine metabolism-related genes, endogenous polyamine levels did not differ substantially. When exogenous polyamines were applied, their roborative effect were detected under all light conditions, but these beneficial changes were correlated with an increase in polyamine content and polyamine metabolism-related gene expression only under blue light. The effect of the polyamines on leaf gene expression under red light was the opposite, with a decreasing tendency. Results suggest that light quality may optimize plant growth through the adjustment of polyamine metabolism at the gene expression level. Polyamine treatments induced different strategies in fine-tuning of polyamine metabolism, which were induced for optimal plant growth and development under different spectral compositions.

Role of phosphorus in Vallisneria natans and biofilm exposure to Pb2+ and Cd2+ stress

Phosphorus (P) could improve the stress resistance and adaptability of submerged macrophytes. This study investigated the physiological and biochemical responses of plants exposed to different P and Pb, Cd concentrations. Alterations of protein synthesis, the DNA methylation (5-mC) level, and the microbial community of biofilm were also evaluated. Results indicated that lower P (0.5 mg·L−1) could promote plant growth and metal enrichment while mitigating the toxicity of metals. Higher P (5.0 mg·L−1) induced a degree of oxidative stress, as confirmed by increased activity of superoxide dismutase, peroxidase, and acid phosphatase, as well as increased malondialdehyde contents. While the variation of metallothionein synthesis and DNA methylation level of the plant was dependent on the level of P and metals in the water. These responses indicated potential mechanisms of P detoxification and intoxication. In addition, more abundant microbial communities were observed in biofilms exposed to P and metals. These findings provide theoretical support for the metal detoxification of P in submerged plants.

Postharvest UV-A radiation affects flavonoid content, composition, and bioactivity of Scutellaria baicalensis root

Ultraviolet A (UV-A) radiation is the main component of solar radiation and can elicit a wide range of biochemical responses in plants. In this study, the effects of UV-A radiation on the flavonoid content, composition, and bioactivity in postharvest Scutellaria baicalensis roots were investigated. The total flavonoid content in S. baicalensis roots under postharvest UV-A radiation increased in a time-dependent manner and reached its maximum value (183.64 g kg−1) on the fifth day. Compared with the control group, the contents of four main flavonoids—baicalin, baicalein, wogonoside, and wogonin increased by 1.68 times, 3.00 times, 2.07 times, and 1.57 times, respectively; and new chemicals including pectolinarigenin, puerarin, isokaempferide, and jaceosidin were detected after 5 d of UV-A radiation. In addition, extracts of S. baicalensis roots exposed to postharvest UV-A radiation showed stronger antioxidant and antibacterial activities than control root extracts. Further correlation analysis revealed that baicalin and baicalein contents in the roots of S. baicalensis after postharvest UV-A radiation were positively correlated with antioxidant activity, while the main flavonoids showed different correlations with various bacteria. These results suggest that the appropriate duration of postharvest UV-A radiation is an effective way to enhance bioactive compound contents and their bioactivities in S. baicalensis roots.

Physiological and Biochemical Responses of Plants and Photoautotrophs to Abiotic Stress: A Comprehensive Review

Abiotic stress factors such as drought, salinity, extreme temperatures, and heavy metals pose significant challenges to plant growth and productivity, impacting global agriculture and ecosystems. Photoautotrophs, including higher plants and algae, exhibit a variety of physiological and biochemical responses to mitigate the detrimental effects of these stressors. This review provides a comprehensive overview of the complex mechanisms underlying plant adaptation to abiotic stress, emphasizing the roles of osmotic adjustment, antioxidant defense, hormonal regulation, and stress-responsive gene expression. Key physiological responses, such as stomatal regulation, root architecture modifications, and chlorophyll stability, are discussed alongside biochemical adaptations, including the accumulation of osmolytes, synthesis of stress proteins, and activation of enzymatic and non-enzymatic antioxidants. Additionally, the review explores recent advancements in genetic and biotechnological approaches for enhancing plant resilience to abiotic stress, highlighting potential applications in agriculture. By examining the intricate interplay between physiological and biochemical pathways in response to stress, this review aims to deepen understanding of adaptive strategies in photoautotrophs, offering insights for the development of stress-tolerant crop varieties essential for food security in changing environments.

Effect of saccharides on secondary compounds production from stem derived callus of Datura inoxia

Datura inoxia is a subshrub plant known for its toxicity which results from the presence of the tropane alkaloid scopolamine and hyoscyamine. Saccharides are one of the most important elicitors that can alter physiological and biochemical responses in plants. This study targeted to increase the production of secondary metabolites in Datura inoxia avoiding genetic alteration and processes using different nontoxic and biodegradable compounds, utilizing calli induced from Datura inoxia stems to observe the effects of mannitol and sorbitol on the production of the two major secondary metabolites, scopolamine and hyoscyamine. Methanolic extract of the whole plant and callus was used to examine the production of two secondary metabolites in Datura inoxia using HPLC in a qualitative and quantitative manner which revealed the increased production of scopolamine and hyoscyamine in calli. The addition of mannitol and sorbitol in the media had a negative effect on both the fresh and dry weight of the calli but production of scopolamine and hyoscyamine increased significantly. In-vitro anti-microbial assay of hyoscyamine against Escherichia coli ATCC25922 and Candida albicans resulted in total inhibition of both the microbes in concentrations as low as 200 µg/ml.