Betel Research Articles

Bioactive Aqueous Fraction of Edible Trigonella foenum‐graecum, Piper betel, and Lagenaria siceraria Homogenate Downregulate the Inflammatory Mediators: In‐silico and In‐vitro Appraisal

AbstractTraditional nutraceutical therapy is a promising approach in the management of inflammatory disorders. Fenugreek, bottle gourd, and betel leaf are rich sources of polyphenolic secondary metabolites. They are abundantly found in the Indian subcontinent for the preparation of healthy traditional functional food. Traditional evidence recommended that these herbs could control inflammation. The study was conducted with those herbs to validate their protective role against inflammatory mediators of arthritis and asthma through in‐silico and in‐vitro models. Phytochemical tests revealed the presence of flavonoids, amino acids, polyphenols, and saponins. Molecular docking was performed against six potential inflammatory bio‐marker proteins, like, LOX‐5, 15‐LOX, PLA2, IL‐6, TNFR1 & TNF‐α with the phyto‐marker compounds of test herbs and found satisfactory binding scores against inflammatory bio‐markers. Extended studies with Fenugreek and betel leaf aqueous fractions showcased 87 % and 91 % inhibition of protein denaturation at 200 μg/ml. Bottle‐gourd and betel‐leaf demonstrated 96 % and 98 % inhibition against 15‐Lipoxygenase at 100 μg/ml. TNF‐α and IL‐6 were dropped significantly by 49 % and 36 % upon treatment of betel leaf at 200 μg/ml from the LPS‐stimulated PBMC cultured cells. Findings may recommend the development of commercial anti‐inflammatory polyherbal phytopharmaceuticals for controlling polyarthritis and inflammatory complications subject to preclinical in‐vivo and clinical investigation.

The paradox of paan: ultural significance, carcinogenic risks, and betel leaf’s anti-cancer properties

The paradox of paan: ultural significance, carcinogenic risks, and betel leaf’s anti-cancer properties

Larvicidal Activity of Red Betel Leaves (Piper ornatum) Ethanolic Extract Against Mosquito Larvae

Larvae are the initial carriers of dengue hemorrhagic fever (DHF) and culex, making them significant in disease transmission. Excessive chemical larvicides pose serious risks to human health, thus driving the development of biological alternatives like red betel leaf extract. This study aimed to assess the efficacy of red betel leaf extract in larval control. Using 300 larvae, concentrations of 5%, 6%, and 7% were tested with three replicates over 24 h. The bioassay involved placing the larvae into a plastic container filled with the extract. It was found that ethanol extract from Piper ornatum leaves contained alkaloids, tannins, saponins, flavonoids, and terpenoids. After 24 h, results revealed 83% mortality at 5%, 98% at 6%, and 100% at 7% of extract concentration. Consequently, the 7% concentration showed the fastest efficacy in killing mosquito larvae (100% in 50 min). The LC50 value, representing the concentration at which 50% of larvae are killed, was determined to be 0.04%. Additionally, the LT50 value, indicating the time required to kill 50% of larvae (based on the LC50 value), was found to be 3.34 hours. In conclusion, red betel leaf extract demonstrated promising larvicidal activity against mosquito larvae. Future research is anticipated to prove its safety via in vitro and in vivo test.

Quantification of Total Phenol Concentration in Ethanol Extracts of Red and Black Betel Leaves Using UV-Vis Spectrophotometric Method

Red betel (Piper crocatum Ruiz & Pav) and black betel leaves (Piper betle var. nigra) are plant varieties known for their medicinal properties. These plants have various contents including flavonoids, essential oils, tannins and polyphenols. The objective of this research is to ascertain the quantity of phenolic compounds present in ethanol extracts of red and black betel leaves. Determination of the levels of ethanol extracts of red and black betel leaves is done quantitatively by the folin-ciocalteu method. This method relies on the formation of a blue complex compound of of phosphomolybdat-phosphotungstat, generated by the reduction of phenolic compounds in an alkaline environment. The measurement is performed using uv-vis spectrophotometry. Gallic acid, derived from hydroxybenzoic acid and identified as a simple phenolic acid, is employed. This stable and pure polyphenolic compound is commonly found in almost all plants. Drawing conclusions from the study results, it can be affirmed that the average total phenol concentration of red betel was 31.87 GAE per gram extract and the average total phenol concentration of black betel leaves was 39.09 mg GAE per gram extract.

Artificial neural networks (ANNs) and response surface methodology (RSM) for optimizing wetting and anti-bacterial properties of woven fabric

This study aims to enhance woven fabric’s antibacterial properties by coating with betel leaf extract using immersion and plasma pre-treatment techniques. It explores nano surface modification and develops mathematical models for inhibition zone values using response surface methodology (RSM) and artificial neural networks (ANNs). We divided the RSM into two groups based on the inputs, model A and model H, and then used artificial neural networks to compare the models. We analyzed fabric treatments using SEM, wetting tests, FT-IR spectrometry, and disc diffusion (Kirby-Bauer) with Staphylococcus aureus to assess nano surface modification and antibacterial properties of fabric-treated with discharged plasma. We compared the optimal inhibition zone values for polyester fabrics with plasma treatment, anti-bacterial coatings, and anti-bacterial and plasma treatment. Fabrics treated with antibacterial coating, plasma treatment, or both exhibited inhibition zones of 2.30 ± 0.50 mm, 3.00 ± 0.50 mm, and 6.40 ± 0.50 mm, respectively. Using RSM model A, wetting properties were predicted with SSE, R-squared, and RMSE values of 0.2402, 0.9999, and 0.2451, respectively. Model A also forecasted inhibition zones for different fabric treatments with R-squared values of 0.899 and 0.9579, and SSE values of 0.8038 and 0.0782, respectively. Additionally, inhibition zones were modeled using RSM model H and ANNs, yielding R-squared values of 0.9128 and 0.97724, respectively. We found that the inhibition zone value using the ANNs model had a better predictive ability than RSM. The new technique can, therefore, be used to enhance the anti-bacterial properties and absorbency of 100% polyester fabrics.

A study on synthesis of amylase, cellulase L-asparaginase enzymes and potassium solubulizing efficiency of rhizobacteria from the Piper betle fields of Guntur District, Andhra Pradesh, India

A study on synthesis of amylase, cellulase L-asparaginase enzymes and potassium solubulizing efficiency of rhizobacteria from the Piper betle fields of Guntur District, Andhra Pradesh, India

Synthesis of zero valent copper/iron nanoparticles using Piper betle leaves for the removal of pharmaceutical contaminant atorvastatin

In this study, bimetallic Cu–Fe nanoparticles were synthesized using the green approach with Piper betle leaves, and the removal efficiency of one of the pharmaceutical compounds, Atorvastatin, was investigated. UV, SEM, FTIR, EDAX, particle size, and zeta potential measurements were used to confirm nanoparticle fabrication. The removal efficiency of Atorvastatin (10 mg/L) by bimetallic Cu–Fe nanoparticles was 67% with a contact time of 30 min at pH 4, the adsorbent dosage of 0.2 g/L, and stirring at 100 rpm. Piper betle bimetallic Cu–Fe nanoparticles have demonstrated excellent stability, reusability, and durability, even after being reused five times. Furthermore, the synthesized bimetallic Cu–Fe nanoparticles demonstrated remarkable antimicrobial properties against gram-negative strains such as Escherichia coli and Klebsiella pneumoniae, gram-positive strains such as Staphylococcus aureus and Bacillus subtilis, and fungi such as Aspergillus niger. In addition, the antioxidant properties of the synthesized bimetallic Cu–Fe nanoparticles were assessed using the DPPH radical scavenging assay. The results indicated that the nanoparticles had good antioxidant activity. Thus, using Piper betle extract to make Cu–Fe nanoparticles made the procedure less expensive, chemical-free, and environmentally friendly, and the synthesized bimetallic Cu–Fe nanoparticles helped remove the pharmaceutical compound Atorvastatin from wastewater.

Antimicrobial efficacy of select medicinal plant extracts from Bangladesh against food‐borne bacterial pathogens

AbstractWe assessed the antimicrobial activity of extracts prepared with four solvents (hexane, acetone, ethanol, water) from 45 medicinal plants used in Bangladesh. Food pathogenic bacteria (Gram‐positive: Bacillus cereus, Listeria innocua, Streptococcus faecalis, and Gram‐negative: Escherichia coli, Pseudomonas aeruginosa, Salmonella enterica, and Shigella sonnei) were tested using a broth microdilution method. The extraction yield was highest (26%) for the water extract of Carica papaya and lowest (0.4%) for the acetone extract of Cynodon dactylon. In general, acetone extracts exhibited much more antibacterial activity than those obtained with the other three solvents (hexane, ethanol, and water). Gram‐positive bacteria were more sensitive than Gram‐negatives. Streptococcus faecalis was the most susceptible to inhibition by examined extracts, whereas E. coli and P. aeruginosa were the most resistant. Most inhibitory concentration‐50 (IC50) values ranged between 101 and 500 μg/mL (64 extracts, 35.5%), followed by the range of 501–1000 μg/mL (40 extracts, 22.2%). Based on IC50, the most effective plants were three species of Piper (Piper nigrum, Piper betle, and Piper chaba), followed by Nigella sativa, Psidium guajava, Syzygium cumini, C. dactylon, and Phyllanthus emblica. In addition, the toxicity of chosen extracts against normal and malignant cell lines was tested; the most effective extracts were toxic against the human lung carcinoma cell line A549, but less toxic against the human Caucasian foetal lung cell line WI26VA4. These findings suggest that some plant extracts could be employed to treat food‐borne bacterial infections, or as herbal preservatives in the food sector.

Synergistic Effect of Henna and Betel Leaf Extract on Dyeing and Antimicrobial Performance of Silk Fabric

Synergistic Effect of Henna and Betel Leaf Extract on Dyeing and Antimicrobial Performance of Silk Fabric

Anti-Infection of Oral Microorganisms from Herbal Medicine of Piper crocatum Ruiz & Pav.

The WHO Global Status Report on Oral Health 2022 reveals that oral diseases caused by infection with oral pathogenic microorganisms affect nearly 3.5 billion people worldwide. Oral health problems are caused by the presence of S. mutans, S. sanguinis, E. faecalis and C. albicans in the oral cavity. Synthetic anti-infective drugs have been widely used to treat oral infections, but have been reported to cause side effects and resistance. Various strategies have been implemented to overcome this problem. Synthetic anti-infective drugs have been widely used to treat oral infections, but they have been reported to cause side effects and resistance. Therefore, it is important to look for safe anti-infective alternatives. Ethnobotanical and ethnopharmacological studies suggest that Red Betel leaf (Piper crocatum Ruiz & Pav) could be a potential source of oral anti-infectives. This review aims to discuss the pathogenesis mechanism of several microorganisms that play an important role in causing health problems, the mechanism of action of synthetic oral anti-infective drugs in inhibiting microbial growth in the oral cavity, and the potential of red betel leaf (Piper crocatum Ruiz & Pav) as an herbal oral anti-infective drug. This study emphasises the importance of researching natural components as an alternative treatment for oral infections that is more effective and can meet global needs.

Formulation and evaluation of antifungal herbal gel using Aloe Vera and Betel leaves extract for the treatment of candidiasis

The primary objective of the current study was to develop and evaluate an antifungal herbal gel for the treatment of candidiasis using an extract from aloe vera and betel leaves. Herbal remedies are now widely used for both medicinal and commercial reasons. The antibacterial gel made from herbal plants works better than synthetic medications and has fewer side effects. Aloe vera belongs to the Asphodelaceae family, which also includes Aloe barbadensis. One species in the Piper betle L. family of Piperaceae is the betel leaf. Aloe vera and betel leaf products, such as plant branches, roots, and leaves, have also been shown to be abundant in phytochemicals. The most popular herbs for treating fungus infections are aloe vera and betel leaves, which also contain potent anti-inflammatory, antibacterial, and antifungal properties. Methods: Hydroalcoholic extract was prepared by the maceration method. Carbopol 940 was used as a gelling agent to create the gel formulation. Candida albicans has been used to test its antifungal properties. Evaluation:The pH, viscosity, homogeneity, grittiness, extrudability, spreadability, antifungal activity and in vitro drug release research were assessed for the optimally designed herbal gel. Results:In comparison to marketed 1% clotrimazole gel 25 mm and marketed herbal gel 20 mm, the aloe vera and betelleaf extract herbal gel (S4 Batch) was found to be brown in color, homogenous, had good extrudability, no grittiness, pH 6.8, viscosity of 3424±0.45, spreadability of 4.3±0.3, and zone of inhibition of 23 mm. After being stored for 60 days, the herbal gel mixture, including extracts of aloe vera, tulsi, and peppermint leaves, did not alter in appearance. The pH has not changed in two months.

Bioassay-guided isolation and identification of antibacterial compounds from Piper betle leaf with inhibitory activity against the Vibrio species in shrimp

ABSTRACT The present study examined the antimicrobial effects of betel leaf (Piper betle) extracts against Vibrio spp. along with the isolation of the most prevalent bioactive components using a bioassay-guided purification. Following a preliminary screening, the ethanolic extract proved the most effective and performed to silica gel chromatography, followed by reversed phase HPLC to isolate the active components. By means of subsequent UHPLC-orbitrap-ion trap mass spectrometry, two compounds were identified as (E)-3-phenylprop-2-enoic acid (cinnamic acid) and [4-(1-hydroxyprop-2-enyl)phenyl] acetate (1’−hydroxychavicol acetate). The compounds showed anti-microbial activity against Vibrio species including V. parahaemolyticus, V. harveyi, V. alginolyticus, V. vulnificus and V. anguillarum. Their antibacterial action was quantified by a microdilution assay showing that cinnamic acid displays more potent activity (IC50, 21–121 µg/mL) compared to 1’-hydroxychavicol acetate (IC50, 24–365 µg/mL). These findings propose the potential use of the betel leaf as a substitute for antibiotics to combat Vibrio infections in shrimp aquaculture.

An Review: On Nutritive Activity and Ayurvedic Property of Piper Betel(Paan)

Piper betel L.comes in family Piperaceae and called Paan leaves in india . Piper betel or Betel vine deep heart shaped .This betel leaf widely shown in Sri Lanka, India, Thailand, Taiwan and other Southeast Asian countries. The oil of paan may be used as an industrial raw material for manufacturing medicines, perfumes, mouth fresheners, tonics, food additives etc. The betel leaves are shows nutritive property as well as carcinogenic property which is very usefull for manufacturing of blood cancer drug which is greatest advantage of betel leaf. Their leaves have been used as a traditional medicine to treat various health conditions. This paan is highly abundant and inexpenxsive, therefore it promotes the further research and industrialization development, including in the food and pharmacuitical industries. P. betle leaves displayed high efficiency on Gram-negative bacteria such as Escherichia coli and Pseudomonas aeruginosa, Gram-positive bacteria such as Staphylococcus aureus, and Candida albicans. Piper betel is mostly use to chew with sliced areca nut, slaked lime, coriander, aniseed, clove, cardamom, sweetener, coconut scrapings etc, but less used remedy. This paan can cure many diseases and reduce the oral cancer which actually happens due to sliced areca nut, slaked lime not because of betel leaves.It contains high amount of nutrient like water,protein , energy , fats , fibres ,calcium etc. y. It is cultivated in hotter and damper part incountry following the traditional methods in India on about 55,000 hectare with an annual production worth about Rs 9000 million. It shows antimicrobial activity, antidiabetic ,Gastroprotective , platelet inhibition activity ,Antifertility like other onens properties ,also they shows the antioxidant , antiallergic , anti coagulant , anti inflammatory , radioprotective , anticancer potential like activities .

SYNTHESIS OF BIODEGRADABLE PLASTIC FROM (Artocarpus heterophyllus Lam), (Zea mays) AND (Piper betle) USING GLYCEROL AS PLASTICIZER

This study aims to produce starch-based bioplastic that is environmentally safe and reduces pollution. This research aims to investigate the effect of betel leaf extract as an antimicrobial agent on the characteristic of bioplastic based on jack fruit seed starch. Bioplastics are the only solution to replace plastics. As a result, these nonbiodegradable plastics remain in the environment for a long time and cause an increase in solid waste production. Starch is one of the major sources in the development of bioplastics. The bioplastic was produced from the starch of jackfruit seed along with glycerol as a plasticizer and analyzed for its physical and mechanical properties. The bioplastic produced was analysed for its antimicrobial activity. The produced bioplastic showed better physio mechanical properties and can be used widely in industries. Keywords: Bioplastics, Glycerol, Jackfruit, Cornleaf, Betel leaf.

The association between betel quid use and oral potentially malignant and malignant disorders in Southeast Asian and Pacific regions: a systematic review and meta-analysis with GRADE evidence profile

BackgroundBetel quid (BQ) chewing is a prevalent habit in the Asian and Pacific regions. It is deeply intertwined within cultural customs, and has been reported to result in oral potentially malignant disorders (OPMDs) and malignant disorders (MDs).ObjectiveWe aim to present a summative and broad overview of the burden that BQ chewing has imposed on the residents of the Southeast Asian, Pacific, and Australasian regions, allowing us to quantify the level of impact it is currently causing on the risk of people developing oral cancer.MethodsThis scoping review and meta-analysis screened databases such as PubMed, MEDLINE, and Google Scholar for publications that investigated the association between BQ and OPMDs and MDs. The search strategy involved MeSH headings relating to BQ, OPMDs, and MDs, and a search for results during the period between January 2010 and June 2023 within the set geographical boundaries of the Southeast Asian and Pacific regions. This systematic review was reported in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Statement for Scoping Reviews (PRISMA-ScR). R software was used to screen outliers. The included studies were further analysed using the Grading of Recommendations Assessment, Development and Evaluation (GRADE) system.ResultsNine articles (n = 19,312 participants) presented odds ratio outcomes from 11 regionally different study groups. We indicated a strong correlation between BQ chewing and the increased risk of OMPDs and MDs. The risk was quantified through meta-analyses with an odds ratio (OR) of 8.18 (5.27–12.72) and an increased OR of 9.93 (7.36–13.39) when the outlier was removed. BQ chewing was further identified within various Australian communities and discovered to be produced locally in North Queensland.DiscussionA meta-analysis of two outcomes revealed substantial heterogeneity and minor evidence of publication bias, thus the association effect was included with and without these articles. The overall GRADE quality of evidence ranged from moderate to very high and highlighted five studies with a high level of imprecision.ConclusionThe lingering high prevalence of BQ in the Southeast Asia and Pacific regions, as well as its rising acceptance among non-ethnic Australians, is alarming and requires prompt and rigorous intervention to prevent the risk of oral cancer.Systematic Review RegistrationPROSPERO (CRD42023429694).

Efficient recovery of allylpyrocatechol from Piper betle using alcohol/salt aqueous two-phase system (ATPS)

The emergence of antibiotic-resistant bacteria presents significant public health threat, triggering a growing demand for natural compounds possessing antibacterial properties within the realm of Complementary and Alternative Medicine (CAM). Our previous research identified allylpyrocatechol from Piper betle as having strong antibacterial and QS inhibition properties. However, isolating phenolic compounds is time-consuming and relies on significant organic solvent use through methods like chromatography. The aqueous two-phase system (ATPS) emerges as an innovative, efficient, and eco-friendly approach for biomolecule recovery. This study assesses ATPS efficiency in allylpyrocatechol recovery from P. betle, optimizing parameters such as phase component, composition, crude load, and pH. Optimal conditions include 22% 1-propanol, 24% ammonium sulfate at pH 7, and a 10% sample load, resulting in a higher yield (5.82±0.04 g/kg plant material) and purity (14.09±0.10%) compared to hydrodistillation (yield: 0.29±0.01 g/kg plant material, purity: 7.17±0.17%). The ATPS extract demonstrates greater antibacterial potency against Staphylococcus aureus (MIC: 500 µg/mL) and Micrococcus luteus (MIC: 500 µg/mL) than hydrodistillation (1000 µg/mL respectively). Additionally, it possesses stronger inhibition effect on violacein (48.00±0.83%) and biofilm production (43.70±1.22%) while hydrodistillation only inhibits by 35.44±0.60% and 33.15±0.66%, respectively. This study underscores ATPS as rapid and effective method for phenolic compound recovery, exemplified by allylpyrocatechol from P. betle.

Powerful Antibacterial for Wound Healing using Betel Leaf Extract

This study investigates the antibacterial properties of betel leaf (Piper Betle L) extract on suture wound infections caused by Staphylococcus aureus. Using 25 Webster mice divided into five groups, the research compared the healing effects of 5%, 10%, and 15% betel leaf extract against a control group and a gentamicin antibiotic group over 8 days. Results, measured with the REEDA scale, indicated that the 15% extract was most effective, achieving optimal healing by the eighth day. These findings suggest that betel leaf extract, particularly at higher concentrations, could serve as a potent alternative to traditional antibiotics for treating bacterial wound infections. Highlights: 15% Extract Most Effective: Achieved complete healing by eighth day. Comparative Study: Betel leaf extract vs. control and gentamicin. Antibacterial Alternative: Potential for treating bacterial wound infections. Keywords: Betel leaf extract, Piper Betle L, wound infection, Staphylococcus aureus, antibacterial

Ethnomedicine of Mukundaras (S. E. Rajasthan)-Plant Remedies Used in Guinea Worm (Naaru) Disease

The communication deals with enumeration of plant species used by tribals and rural people residing in the vicinity of Mukundara ranges, Jhalawar district (S.E. Rajasthan), as hebral medicine for Guinea worm (Naaru) disease. This disease is very common in tribal and rural areas of South East Rajasthan, and is communicated through contaminated water. The 12 plant species, along with their plant parts used are - Arisaema tortuosum (Tuber), Aristolochia bracteolata (Root), Blumea lacera (leaf), Calotropis procera (Root), Cleome viscosa (leaf), Ferula asafoetida (Oleoresin Root), Hemiadelphis polyspermus (Root), Piper betel (leaf), Psoralea corylifolia (Seed), Pueraria tuberosa (Root), and Ricunus communis(Root). Enumeration contains, Plant's name, Family, Local name, Voucher specimen and Phenology along with brief notes on preparation of plant drug, the plant part used, amount and periodicity of dosage given.Brief notes on causal organism Dracunculus medinensis (Guinea worm) also given along with etiology, symptoms and mode of infection.

Optimized encoder-decoder cascaded deep convolutional network for leaf disease image segmentation.

Nowadays, Deep Learning (DL) techniques are being used to automate the identification and diagnosis of plant diseases, thereby enhancing global food security and enabling non-experts to detect these diseases. Among many DL techniques, a Deep Encoder-Decoder Cascaded Network (DEDCNet) model can precisely segment diseased areas from the leaf images to differentiate and classify multiple diseases. On the other hand, the model training depends on the appropriate selection of hyperparameters. Also, this network structure has weak robustness with different parameters. Hence, in this manuscript, an Optimized DEDCNet (ODEDCNet) model is proposed for improved leaf disease image segmentation. To choose the best DEDCNet hyperparameters, a brand-new Dingo Optimization Algorithm (DOA) is included in this model. The DOA depends on the foraging nature of dingoes, which comprises exploration and exploitation phases. In exploration, it attains many predictable decisions in the search area, whereas exploitation enables exploring the best decisions in a provided area. The segmentation accuracy is used as the fitness value of each dingo for hyperparameter selection. By configuring the chosen hyperparameters, the DEDCNet is trained to segment the leaf disease regions. The segmented images are further given to the pre-trained Convolutional Neural Networks (CNNs) followed by the Support Vector Machine (SVM) for classifying leaf diseases. ODEDCNet performs exceptionally well on the PlantVillage and Betel Leaf Image datasets, attaining an astounding 97.33% accuracy on the former and 97.42% accuracy on the latter. Both datasets achieve noteworthy recall, F-score, Dice coefficient, and precision values: the Betel Leaf Image dataset shows values of 97.4%, 97.29%, 97.35%, and 0.9897; the PlantVillage dataset shows values of 97.5%, 97.42%, 97.46%, and 0.9901, all completed in remarkably short processing times of 0.07 and 0.06 seconds, respectively. The achieved outcomes are evaluated with the contemporary optimization algorithms using the considered datasets to comprehend the efficiency of DOA.

Effects of montmorillonite on the loading and release of Piper betle L. extract applied for wound dressing based on chitosan/polyvinyl alcohol

In this study, we investigate the influence of montmorillonite (MMT) on the loading and release of Piper betle L. extract (PLE)–a medicinal herb containing active secondary metabolites with antibacterial, antioxidant, and anti–inflammatory effects. MMT (1 %, 3 %, 5 %) was blended into the chitosan/polyvinyl alcohol (CS/PVA) biocomposite film by the solution evaporation method, and then PLE was loaded onto this biocomposite using the immersion method. The tensile strength and the ability to absorb exudates of the CS/PVA film improved with the increase in MMT content. The MMT 3 % film was considered to have the best properties: good mechanical properties with a tensile strength of 27.44 ± 0.27 MPa and elongation at break of 14.57 ± 0.30 %, potential for wound dressing due to its ability to absorb wound exudate (swelling degree 61.70 ± 0.30 %) and a suitable water vapor transmission rate (1999 ± 47 g/m2·d). The presence of MMT (1 %, 3 %, 5 %) in the CS/PVA film led to an increase in the PLE loading efficiency of the films compared to the film without MMT, up to 1.65, 1.73, and 1.87 times, respectively. The MMT 3 % and 5 % films also exhibited a sustained PLE release effect for up to 24 h. MMT increased PLE bioavailability through bioactivity tests: antibacterial activity against both E. coli and S. aureus, antioxidant activity, effective healing of 2nd–degree burn wounds, and biocompatibility with the L929 fibroblasts cell line. The combination of physicochemical properties and biological activities proved that the MMT/PLE drug delivery system based on the CS/PVA biocomposite is promising for wound dressing.