The use of very high-energy gamma and electron radiation for the treatment of cancer in man poses the question of the eventual biological effects on the tissues involved. Does radiation of such type cause a different response qualitatively and/or quantitatively in comparison to standard x-ray exposure? Investigation of this problem is a prerequisite to the application of high-energy radiation therapy. Though many studies have been conducted (1–6), research is continued in the interest of improving treatment technic and enhancing the response of the malignant tissues to radiation. Biological effectiveness has been observed to vary with the energy of the irradiating agent (7–9, and others). Quastler (5), Haas (1), and others have demonstrated such variation in biological objects irradiated with betatron x-rays of 22 MEV and electrons of similar energy. The difference of the effects as compared with those of 400- and 200-kv x-ray exposures seems to be of an entirely quantitative nature; no qualitative changes could be detected.2 The purpose of the experiments to be recorded here was to establish the degree to which fractionated x-ray and electron dosages influence the survival and other biological activities of rats in comparison to equal amounts of ionizing radiation of lower energy. Methods For these experiments, male albino rats of the Holtzman strain were used. A two-week period was allowed for conditioning the animals to their new environment before the first irradiation took place. The rats were sorted at random into several groups, each consisting of 22 animals. One group was used for exposure to 400-kv x-rays generated by a General Electric 400-kv unit (h.v.l., 2.1 mm. Cu). A second group was irradiated by a 22-MEV betatron x-ray source (h.v.l., 14 mm. Pb), while a third received electron radiation of 20 MEV energy.3 A final group was kept as a control but, except for exposure to radiation, was handled exactly as were the other animals. From each group 11 rats were treated simultaneously in a rotating Lucite cage, described elsewhere (11). This allowed more uniform irradiation and reduced errors due to individual dosage variations to a minimum. It also resulted in a saving of time since the wheel was placed at such a distance from the radiation source that only 6 r/min. were delivered to the animals. In this location the entire rotating cage (1 rpm) was uniformly covered by the 400-kv and 22-MEV x-ray beams. For electron beam irradiation, only sectional areas of the wheel could be irradiated. The electron beam was defined by a Lucite and brass shield having an opening identical in configuration with each of the twelve individual compartments in the Lucite wheel.
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