Antioxidant Activity Of Berries Research Articles

Influence of freeze-drying parameters on the content of polyphenols and anthocyanins in strawberries

Strawberry berries are a valuable product rich in vitamins, anthocyanins, and polyphenolic compounds that have antioxidant effects on human health. However, due to the deterioration and seasonal availability, the issue of their long-term storage is highly relevant. Freeze-drying, a modern preservation method, preserves their properties during storage. Since the drying process can affect the appearance, nutrient content, and antioxidant activity of berries, the purpose of this study was to investigate the effects of freeze-drying parameters on the content of anthocyanins and polyphenols in Albion variety strawberries. The heating temperatures of the freeze-drying were 35°C, 40°C, 45°C, and 50°C, and the sublimation process times were 18, 20, and 22 hours. Optimal conditions were found: a shelf temperature of 45°C and 18 hours of drying. These conditions preserved both the strawberries' initial qualities and their high anthocyanin content, with only an 8.4% decrease. Similarly, polyphenol content decreased by 3.9%, while the strawberries maintained strong antioxidant activity.

Fruit maturity stage in relation to content of polyphenols, flavonoids and antioxidant activity of selected clones of Lonicera kamtschatica (Sevast.) Pojark

The article is dealing with evaluation of four fruit maturity stages of 6 selected clones (LKL-3, LKL-14, LKL-19, LKL-33, LKL-96 and LKL-103) of Lonicera kamtschatica (Sevast.) Pojark in relation to total polyphenols, flavonoids content and antioxidant activity of berries. The experiment in 5 replications of each clones has been established in the conditions of Botanical Garden in Nitra (Slovak republic)in 2016. The results of analyses proved that in respect of the total polyphenols content (TPC), the content of flavonoids (FC) and the antioxidant activity the most valuable clones at the stage of full ripened fruit were following: LKL-14 (8.77; 2.57 g/kg FW; 9.07 g/kg), LKL-33 (6.77; 2.84 g/kg FW; 9.16 g/kg) and LKL -3 (8.19; 2.61 g/kg FW; 8.94 g/kg). The results of statistical analyses by one-way analysis of variance proved significant differences among assayed clones in total flavonoids and antioxidant activity of fruit but no differences among content of total polyphenols and assayed stages of fruit maturity.

BUNCH NUMBER AND ANTIOXIDANT ACTIVITY IN ‘SAHEBI’ AND ‘HALAGHO’ GRAPES AFFECTED BY PRUNING INTENSITY AND CANE LENGTH

Pruning methods can primarily affect grape production at various levels. Of the commonly affected features by pruning, the number of bunches has known to have a significant effect on the relation between the functions of assimilate sources and sinks. Further, fruitfulness can significantly be negatively influenced by shading. In this study, we aim to study the impact of 4, 6 and 8 buds per cane with pruning intensity as light, moderate and severely pruned and their interaction in the course two consecutive years, 2017 and 2018 in ‘Sahebi’ and ‘Halagho’ (both seeded red table grape cultivars). In 2018, lightly pruned ‘Halagho’ and having 8 buds per cane increased number of bunches by 54.00, which had the highest value. Results showed that there is apical buds bear more fruit by comparison to the buds with vegetative nature, 1 to 4 first buds. Based upon the outcomes of this study, it could be concluded that due to the stresses imposed by the pruning of the vines, therefore, the chemical compounds increases in order alleviate the negative aftermaths . In 2018, whole berry antioxidant activity in both cultivars was significantly lessened, and this decline was higher in ‘Saahebi’. The number of bunches was increased by increasing cane length or bud load/cane. Overall, the pruning program has an impactful role in bunch number as well as antioxidant activity and in the long-run on accumulated reserves, which makes choosing and applying a specific pruning method undeniably important. Thus, emphasize the importance of further research in this field.

Bunch number and antioxidant activity in ‘Sahebi’ and ‘Halagho’ grapes affected by pruning intensity and cane length

Pruning methods can primarily affect grape production at various levels. Of the commonly affected features by pruning, the number of bunches has known to have a significant effect on the relation between the functions of assimilate sources and sinks. Further, fruitfulness can significantly be negatively influenced by shading. In this study, we aim to study the impact of 4, 6 and 8 buds per cane with pruning intensity as light, moderate and severely pruned and their interaction in the course two consecutive years, 2017 and 2018 in ‘Sahebi’ and ‘Halagho’ (both seeded red table grape cultivars). In 2018, lightly pruned ‘Halagho’ and having 8 buds per cane increased number of bunches by 54.00, which had the highest value. Results showed that there is apical buds bear more fruit by comparison to the buds with vegetative nature, 1 to 4 first buds. Based upon the outcomes of this study, it could be concluded that due to the stresses imposed by the pruning of the vines, therefore, the chemical compounds increases in order alleviate the negative aftermaths . In 2018, whole berry antioxidant activity in both cultivars was significantly lessened, and this decline was higher in ‘Saahebi’. The number of bunches was increased by increasing cane length or bud load/cane. Overall, the pruning program has an impactful role in bunch number as well as antioxidant activity and in the long-run on accumulated reserves, which makes choosing and applying a specific pruning method undeniably important. Thus, emphasize the importance of further research in this field.

Polyphenolic profile and antioxidant activities of Madeiran elderberry (Sambucus lanceolata) as affected by simulated in vitro digestion

The aims of this study were twofold: a) to provide a detailed report on the phenolic composition and antioxidant activity of fresh berries and leaves of Sambucus lanceolata (Madeiran elderberry); b) to study the effects caused by a simulated in vitro digestion on the composition and antioxidant activity of the berries and leaves.Seventy-seven phytochemicals, mainly polyphenols, were identified in the methanol extracts of fresh berries and leaves, with the content of polyphenols higher in berries (27.2mg·g−1 dry extract, DE) than in leaves (25.9mg·g−1 DE). Anthocyanins were dominant in berries, while hydroxycinnamic acids (HCAs) and flavonols were abundant in leaves. Higher antioxidant activities were found in leaves than in berries, using several in vitro assays. After the simulated in vitro digestion, the levels of polyphenols were significantly reduced, in particular those of berries (81.8% decrease). Anthocyanins were the most affected compounds during the simulated digestion. However, despite the significant loss of phenolic compounds during digestion, methanol extracts of digested berries and leaves were still able to scavenge free-radicals. Hence, the consumption of leaves and/or berries of S. lanceolata may help prevent oxidative stress.

Anthocyanin Profile and Antioxidant Activity of Various Berries Cultivated in Korea

This study examined the anthocyanin composition and antioxidant activity of various berries cultivated in Korea: blueberry, crowberry, Korean black raspberry, mulberry, and strawberry. The anthocyanins in berries were identified by high-performance liquid chromatography (HPLC) analysis, and each component was quantitatively analyzed. Furthermore, the antioxidant activity of berries was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical-scavenging, 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation decolorization, oxygen radical absorbance capacity (ORAC), and ferric ion reducing antioxidant (FRAP) assays. The results revealed that the total content of anthocyanins in crowberry was 35.1 mg/g of extract, which was higher than that in the other four major berry species (1.9-27.7 mg/g of extract). Nineteen anthocyanins were identified in the various berries. The major anthocyanins of crowberry were cyanidin-3-galactoside and delphinidin-3-galactoside, and those from Korean black raspberry were cyanidin-3-rutinoside and cyanidin-3-sambubioside-5-rhamnoside. These two berries also had relatively strong antioxidant activity accompanied by high total polyphenol contents. Thus, consumption of crowberry and Korean black raspberry may be beneficial in reducing the risk of developing lifestyle-related chronic diseases because of their strong antioxidant activity.

The effects of bud load and regulated deficit irrigation on sugar, organic acid, phenolic compounds and antioxidant activity of Razakı table grape berries

This study aims at assessing the effects of increased bud load and irrigation applications on berry quality of the Razaki table grape. Two Regulated Deficit Irrigation (RDI) having different irrigation levels (RDI-I and RDI-II) based on the growth stages, in addition to a non-irrigated control treatment together with two different bud load practices (K-normal and 2K-two-fold buds of the normal) were examined for their effects on quality attributes such as sugar and organic acids contents, phenolic compounds as well as antioxidant capacity of the berries. The non-irrigated vines had highest sugar level (198.86 g/kg) in the first year (2013) of the experiment whilst the sugar content of the berries was increased with irrigation (RDI-II) in 2014. However the highest organic acid (7.10 g/kg) was recorded from the RDI-II treatment in 2013 whereas those of from non-irrigated vines were highest (7.81 g/kg) in 2014. Considering the sugar and organic acid content of the berries, bud load effects were not significant. The total phenolic acids were higher under non-irrigated and 2K bud load conditions. Antioxidant activity of berries was increased with RDI-I irrigation and 2K practices in the first year (2013) although no significant effect was recorded in the second year of the experiment. In all applications, glucose among the sugars, tartaric acid among the organic acids, catechin and epicatechin among the phenolic compounds were detected to be higher compared to other components in berries.

Effect of Modified Atmosphere Packaging (MAP) on the Quality of Sea Buckthorn Berry Fruits during Postharvest Storage

AbstractThe effect of modified atmosphere packaging (MAP) on the quality of the berry fruits of sea buckthorn (SBT) during refrigerated storage was investigated. SBT berries were packaged in 160 and 525 oxygen transmission rate (OTR) films or in vented clamshell containers (air control) and stored at 10C for 10 days. Changes in the quality of the berries were determined by the evaluation of their headspace composition, moisture content, dry weight, pH level, color, total soluble solids, texture and antioxidant activity. The results indicated that the 160 OTR film presented good competency to maintain a proper atmosphere for storage. Both the 160 and 525 OTR films showed better capability for retaining the firm and springy texture of the berries, as well as their weight. The total soluble solids of 160 OTR packed fruits remained a stable value. The pH of SBT berries packed in different films and control samples increased in general during storage compared with the initial pH of fruits. The antioxidant activity of the berries was higher in MAP samples, especially those in the 160 OTR packages.Practical ApplicationsThis is the first study on the storage characteristics of berry fruit of sea buckthorn (SBT) under modified atmosphere packaging conditions. Film of 160 oxygen transmission rate (OTR) effectively maintained the initial quality parameters and antioxidant activity of berries by slowing down the respiration intensity. It may be because 160 OTR provided excellent CO2/O2 exchange performance to supply a desired gaseous environment of berry fruit. Thus, 160 OTR could be used as a potential packaging film for SBT berry on maintaining fruit quality and antioxidant activity. Furthermore, 160 OTR to different food systems of vegetables, mushrooms and juicy fruits can greatly maintain the nutritional value, product quality and fresh supply. The results of the present study can also serve as an assistive technology combined with other preservation techniques such as ice temperature, controlled atmosphere storage and so on to achieve desirable storage performance.

In Vitro Studies on the Relationship Between the Antioxidant Activities of Some Berry Extracts and Their Binding Properties to Serum Albumin

The aim of this study was to investigate the possibility to use the bioactive components from cape gooseberry (Physalis peruviana), blueberry (Vaccinium corymbosum), and cranberry (Vaccinium macrocarpon) extracts as a novel source against oxidation in food supplementation. The quantitative analysis of bioactive compounds (polyphenols, flavonoids, flavanols, carotenoids, and chlorophyll) was based on radical scavenging spectrophometric assays and mass spectrometry. The total phenolic content was the highest (P < 0.05) in water extract of blueberries (46.6 ± 4.2 mg GAE/g DW). The highest antioxidant activities by 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay and Cupric reducing antioxidant capacity were in water extracts of blueberries, showing 108.1 ± 7.2 and 131.1 ± 9.6 μMTE/g DW with correlation coefficients of 0.9918 and 0.9925, and by β-carotene linoleate assay at 80.1 ± 6.6 % with correlation coefficient of 0.9909, respectively. The water extracts of berries exhibited high binding properties with human serum albumin in comparison with quercetin. In conclusion, the bioactive compounds from a relatively new source of gooseberries in comparison with blueberries and cranberries have the potential as food supplementation for human health. The antioxidant and binding activities of berries depend on their bioactive compounds.

Effect of drying techniques on the retention of antioxidant activities of Saskatoon berries

The main objective of this research was to compare the retention of antioxidant activity and total anthocyanin content of Saskatoon berries dried by freeze drying, microwave-vacuum drying, thin layer hot air drying and vacuum drying. Antioxidant activity of berry samples was determined by DPPH radical scavenging and ABTS radical scavenging, and the pH differential method was used to determine total anthocyanin content of the berry samples. The results showed that the freeze dried Saskatoon berries exhibited the highest retention of anthocyanin and antioxidant activity among the dried samples, followed by microwave-vacuum dried berries, thin layer hot air dried berries and vacuum dried berries. There were significant differences between the berry samples at P&lt;0.05. DPPH radical scavenging and ABTS radical scavenging were correlated linearly with an R2 value of 0.99 at P&lt;0.05 showing their effectiveness for the determination of the antioxidant activity of the Saskatoon berries. However, the DPPH radical scavenging assay was more effective than the ABTS radical scavenging assay. The results also showed that antioxidant activity of the berries was highly correlated with the total anthocyanin content of the fruit. The reduction of anthocyanin in dried berry samples was linearly correlated with the reduction of DPPH radical scavenging with an R2 value of 0.97 at P&lt;0.05 and, also, linearly correlated with the reduction of ABTS radical scavenging with an R2 value of 0.88 at P&lt;0.05.

Effect of Drying Methods with the Application of Vacuum Microwaves on the Bioactive Compounds, Color, and Antioxidant Activity of Strawberry Fruits

The objective of this study was to evaluate the application of vacuum-microwave drying (240, 360, and 480 W) in the production process of dehydrated strawberry and to compare and contrast the quality of these dehydrated strawberries in terms of their polyphenol compounds, concentration of some heat liable components, and color to that of freeze-dried, convective, and vacuum-dried strawberry. Thus, the effect of vacuum-microwave drying and other drying methods on the antioxidant activity of berries was evaluated. Whole fresh and dried fruits were assessed for phenolics (anthocyanins, flavanols, hydroxycinnamic acids, and flavonols), ascorbic acid, and antioxidant activity (all parameters were calculated on a dry matter basis). Analysis of data shows that ellagic acid and flavanol changes were affected by drying techniques and cultivar. Drying destroyed anthocyanins, flavanols, and ascorbic acid, and there was a significant decrease in antioxidant activity. The most striking result was that conventional and vacuum drying decreased antioxidant activity in both cultivars, whereas contradictory results were found for vacuum-microwave processed strawberry. This study has demonstrated that vacuum-microwave drying, especially at 240 W, can produce high-quality products, with the additional advantage of reduced processing times, compared to other processes such as freeze-drying.

Influence of in vitro and ex vitro propagation on anthocyanin content and anti-oxidant activity of lingonberries

SummaryThe fruit yields, anthocyanin contents and anti-oxidant activities of lingonberry (Vaccinium vitis-idaea L.) cultivars ‘Splendor’ and ‘Erntedank’ were studied after being propagated either by conventional softwood cuttings (SC) or by in vitro adventitious shoot regeneration from excised leaves of micropropagated shoots (TC). A significant interaction between propagation method and cultivar was observed for berry anti-oxidant activity. After 4 years of growth, the TC plants produced berries with more anti-oxidant activity, although berry diameter, number and yield per plant were higher in SC plants. The three dominant lingonberry anthocyanins (cyanidin-3-galactoside, cyanidin-3-glucoside and cyanidin-3-arabinoside) were not affected by propagation method. ‘Erntedank’ out-yielded ‘Splendor’ for berry production, and produced more, but smaller berries with a higher anti-oxidant activity than those of ‘Splendor’.