The aim of the study was to compare the functional and sexual outcomes of bipolar transurethral vaporization of the prostate (TUVP) and bipolar transurethral resection of the prostate (TURP) in patients with infravesical obstruction due to BPH. This is a randomized controlled study conducted in Urology Department of Beni-Suef University hospital on 72 patients aged above 50 years with infravesical obstruction and indicated for intervention, patients were divided into 2 Groups, Group A 36 patients operated on by bipolar TUVP and Group B 36 patients operated on by bipolar TURP. The TUVP group exhibited superior intraoperative and early postoperative outcomes, including shorter operative time (70.28 vs. 87.08 min, p < 0.001), less blood loss (Hb drop 0.37 vs. 1.51 g/dl, p < 0.001), and reduced catheterization/hospital stay (1.19 vs. 2.28 days, p < 0.001) compared to TURP while comparable results in modified Clavien classification of complications. At 1-month follow-up, both procedures showed similar improvements in international prostatic symptom score (IPSS), flow rates (Qmax), and residual urine volume, though retrograde ejaculation was more frequent with TURP (72.2% vs. 41.7%, p < 0.001). By 3-12 months, IPSS and Qmax remained comparable, with no significant differences in erectile function (IIEF). However, TURP showed clinically relevant (though non-significant) advantages in long-term symptom control (IPSS 4.03 vs. 4.92). and urinary flow, suggesting potentially greater durability than TUVP. Bipolar TUVP demonstrates notable advantages in early postoperative recovery, including shorter operative times, reduced blood loss, and faster convalescence, positioning it as a minimally invasive alternative for eligible patient. However, TURP may offer superior long-term symptom relief and urinary flow rates despite carrying a higher risk of retrograde ejaculation.

Background/Objectives: Non-variceal upper gastrointestinal bleeding (NVUGIB) remains a major clinical emergency, particularly among patients receiving antiplatelet or anticoagulant therapy, whose use has increased substantially in recent years. This study aimed to evaluate the clinical characteristics, endoscopic findings, risk stratification, and outcomes of NVUGIB in patients receiving antithrombotic therapy, and to compare the predictive performance of commonly used prognostic scores. Methods: This prospective cohort study included 89 patients receiving antithrombotic therapy who presented with NVUGIB at Beni-Suef University Hospitals between March 2023 and March 2025. Clinical presentation, laboratory findings, and endoscopic characteristics were recorded. Risk stratification was assessed using Glasgow-Blatchford (GBS), Rockall, Baylor, AIMS65, ABC, and PNED scores. The optimal cut-off values for prediction of rebleeding and mortality were determined using receiver operating characteristic (ROC) analysis and the Youden index. Area under the curve (AUC) values were reported with 95% confidence intervals. Results: Endoscopy revealed that peptic ulcers were the most common lesion (41/89, 46%), followed by erosive disease (27/89, 30%), with the stomach being the most frequently involved site (76.5%). Rebleeding occurred in 16 patients (18.0%), while mortality was observed in 2 patients (2.2%). The Glasgow-Blatchford score demonstrated the most consistent performance for predicting rebleeding, with an optimal cutoff value of 5.5 (derived using the Youden index), yielding 92.9% sensitivity and 78.8% specificity. For mortality prediction, AIMS65, ABC, and PNED scores showed very high AUC values, although these findings should be interpreted cautiously due to the small number of mortality events (n = 2). No statistically significant difference in rebleeding or mortality was observed between single and dual antithrombotic therapy, although patients receiving dual therapy required longer hospitalization and more transfusion units. Conclusions: In patients with antithrombotic-related GI bleeding, ulcers and erosions predominate, with minimal differences between single and dual therapy outcomes. Concomitant NSAID use trends toward higher mortality. Glasgow-Blatchford score offers optimal performance for both rebleeding and mortality prediction, with a cutoff of 5.5 providing excellent sensitivity (92.9%) and specificity (78.8%) for rebleeding risk assessment.

Background: Poor ovarian reserve (POR) affects IVF/ICSI outcomes, according to the Bologna criteria, factors like older age, a low number of oocytes retrieved, decreased antral follicle count (AFC) and Low levels of anti-Müllerian hormone (AMH) suggest a poor ovarian response (POR). Growth hormone (GH) might enhance follicle development through IGF-1, potentially boosting the ovarian response in individuals with POR. Aim: The objective is to examine how growth hormone adjuvant therapy affects outcomes of ICSI procedures for women with diminished ovarian reserve. Patients and methods: Prospective study focused on patients diagnosed as POR and underwent ICSI, and it was conducted at BeniSuef University from February 2022 through January 2024. Participants were allocated into 2 groups: (A) a control group without adjuvant therapy and (B) a group that received GH adjuvant therapy. Results: There were no initial differences in age, BMI, hormones, or AFC between the groups. The GH group experienced fewer days of stimulation (11.67±1.5 compared to 12.87±1.2, P&lt;0.001), required a lower dose of gonadotropins (4828±1005 versus 5207±1018 IU, P=0.008), and had more number of metaphase II oocytes (2.99 ± 1.8 compared to 2.2±1.1, P=0.002). No variations in peak E2 levels, endometrial thickness, embryo transfer rates, or rates of chemical/clinical pregnancy (19.4% versus 14.8%, P=0.437), ongoing pregnancy, or miscarriage. Conclusion: Growth hormone (GH) adjuvant therapy is effective in reducing the duration and dosage of stimulation while increasing mature oocytes count in patients of poor ovarian response (POR). However, it does not improve pregnancy rates. To determine the optimal dosing and timing, large-scale randomized controlled trials (RCTs) are required.

Purpose: To evaluate macular changes by optical coherence tomography (OCT) following pars plana vitrectomy (PPV) with silicone oil (SO) tamponade in primary rhegmatogenous retinal detachment (RRD). Methods: This prospective interventional study included 50 eyes with recent RRD treated at Beni Suef University Hospital. All patients underwent complete ophthalmological examination, PPV with SO tamponade, and OCT (Optovue RTVue 100 XR Avanti) within two weeks and again at three months postoperatively to assess central macular thickness (CMT) and structural changes. Results: Mean CMT decreased significantly from 261.5 ± 22.1 µm at two weeks to 245.1 ± 40.2 µm at three months (P=0.001). OCT findings included focal photoreceptor loss (12%), intraretinal cysts (10%), atrophic maculopathy (12%), attenuated IS/OS junction (4%), epiretinal membrane (6%), cystoid macular edema (4%), and macular hole (4%). Conclusion: PPV with SO tamponade is a safe and effective option for primary RRD, while OCT provides valuable detection of postoperative complications. Larger multicenter studies are needed to confirm these findings.

Background: Stress urinary incontinence (SUI) is a prevalent condition among women that significantly affects quality of life. Aim: To evaluate the efficacy and feasibility of transvaginal ultrasound-guided periurethral platelet-rich plasma (PRP) injection in the treatment of female stress urinary incontinence. Methods: This prospective clinical study included 54 women diagnosed with SUI who were treated at Beni-Suef University Hospital between February 2023 and March 2024. All patients underwent transvaginal ultrasound-guided periurethral PRP injection, with a second injection administered after three months when indicated. Treatment outcomes were assessed using validated Arabic versions of the International Consultation on Incontinence Questionnaire–Short Form (ICIQ-UI-SF), ICIQ-LUTSqol, stress test, and pad weight test over a follow-up period of nine months. Results: After completion of treatment, 18.5% of patients were cured and 22.2% showed improvement, while 59.3% showed no response. Treatment efficacy was highest in patients with mild SUI (100% after the second injection), moderate in moderate SUI (36%), and lowest in severe SUI (31.3%). Significant improvement in quality of life scores was observed, particularly in mild and moderate cases. Younger age, lower parity, and cesarean delivery were associated with better outcomes. Conclusion: Transvaginal ultrasound-guided PRP injection is a safe and feasible minimally invasive treatment option for female stress urinary incontinence, with the greatest benefit observed in mild cases. Further randomized controlled trials are recommended to validate these findings and optimize patient selection

Background: Anemia during gestation is a major public health problem with 40.1% of pregnancies worldwide suffering from the condition. Anemia can have serious adverse health consequences for the mother and child. Objectives: To evaluate the occurrence of anemia in the 2nd and the 3rd trimesters of gestation in Beni-Suef governorate. Methods: This cross-sectional observational research has been carried out on a total of 500 pregnancies who presented during the 2nd, or 3rd trimester of their pregnant women at hematology clinics or outpatient clinic in Beni-suef University hospital. Results: There were high occurrence of anemia (87.6%) among pregnancies in their second and third trimesters, with iron deficiency anemia (IDA) being the most common type (65.2%). The mean age of the study participants was 28.91±6.96 years. 49.0% of the study participants were in 2nd trimester while 51.0% were in the 3rd trimester. The mean HB, MCV, MCH, Iron level, ferritin, TIBC and T SAT showed high statistically significant variance among both groups (p value below 0.001). There were statistically insignificant variances among anemic pregnant females and non-anemic regarding Age, HR, TLC, PLT, liver and renal function, BMI or gestational trimester (p value above 0.05). Conclusion: High prevalence of anemia among pregnancies in their 2nd and 3rd trimesters. Iron deficiency is responsible for the majority of cases of anemia that occur through gestation

Sickle cell disease (SCD) is a hereditary hemoglobin disorder marked by the polymerization of deoxygenated sickle hemoglobin (HbS), causing vaso-occlusion, hemolysis, and persistent inflammation, leading to substantial morbidity and mortality. Recent studies have found hypoxia-inducible factors (HIFs) to be crucial regulators in cellular reactions to low oxygen conditions, commonly seen in different tissues impacted by SCD. The activation of HIFs is essential in regulating erythropoiesis, vascular stability, and inflammation, which are all key to the pathophysiology of SCD. This review examines the molecular interactions between HIFs and essential disease processes, emphasizing their role in the intricate clinical presentations of SCD. HIFs, especially HIF-1, control various genes that play a role in erythropoiesis and the vascular reaction to low oxygen levels. In SCD, the rise in tissue hypoxia worsens erythropoietic dysregulation and leads to the atypical generation of sickled red blood cells, which are susceptible to early hemolysis. Moreover, HIFs stimulate inflammatory pathways by increasing the production of cytokines and adhesion molecules, resulting in endothelial dysfunction and microvascular blockages. The resulting inflammatory reaction drives a harmful cycle of blood vessel harm and organ injury, which is characteristic of complications in SCD. Peer Review History: Received 22 December 2025; Reviewed 9 January 2026; Accepted 17 February; Available online 15 March 2026 Academic Editor: Dr. Essam Mohamed Eissa, Beni-Suef University, Egypt, dressamceutics@yahoo.com Reviewers: Dr. Essam Mohamed Eissa, Beni-Suef – 32 Tahrir St, Egypt, dressamceutics@yahoo.com Dr. Julie Ann S. Ng, Blk 18 Lot 6 Grandville 3 Subdivision Mansilingan, Bacolod City, Philippines. julieann_ng@yahoo.com

The immune systems of children that are still developing are particularly susceptible to being affected by long-lasting infections like HIV. Children infected perinatally show indications of immune aging or immune senescence significantly sooner than their uninfected counterparts, even with the prompt start of combination antiretroviral therapy (cART). This rapid aging process is marked by persistent immune activation, thymic impairment, disrupted hematopoiesis, and the premature buildup of senescent and weary immune cells. These changes jeopardize immune function during a vital period of growth and development. At the heart of HIV-related senescence in children is the ongoing condition of systemic inflammation and immune imbalance, despite the presence of viral suppression. Thymic involution diminishes the production of naïve T-cells, whereas telomere shortening and increased levels of senescence markers like p16^INK4a and PD-1 indicate cellular tiredness. These immunological changes have significant clinical consequences, including heightened vulnerability to infections, insufficient vaccine efficacy, and premature development of non-AIDS-related comorbidities like cardiovascular and neurocognitive issues. Peer Review History: Received 6 December 2025; Reviewed 11 January 2026; Accepted 13 February; Available online 15 March 2026 Academic Editor: Dr. Sally A. El-Zahaby, Pharos University in Alexandria, Egypt, sally.elzahaby@yahoo.com Reviewers: Dr. Sabah Hussien El-Ghaiesh, Tanta University, Egypt, s.ghaiesh@gmail.com Dr. Sameh Abdelmoneem Mohammed Ali, Faculty of Pharmacy, Beni-Suef University, Egypt, same7_pharma18@yahoo.com

Background: Breast tissue density is higher in younger women with lower body mass indexes, especially during pregnancy, nursing, or hormone therapy. Increased obesity raises breast cancer risk, linked to more aggressive tumor characteristics. Physical exercise can reduce this risk, particularly for women with a family history of the disease, by lowering exposure to endogenous sex hormones and modifying immune responses, even in those without such a history. Aim: Evaluate the effect of an educational program on female workers’ general knowledge regarding breast cancer. Subjects and Methods: Design: A quasi-experimental design was utilized in conducting the study. Sample & Settings: A purposeful sample of 323 working women from Beni-Suef University aged 18-60 years. Tools: A structured interviewing questionnaire sheet, women's general knowledge about breast cancer, and supportive material (Arabic booklet). Results: 45.8% have a university education, 75.9% are married, and 69.0% are urban residents. A statistically significant improvement in female workers’ knowledge regarding total general information about breast cancer during the posttest as p≤0.05 was found. The 1.5% of females who had university education had good knowledge before the program, which increased to 30.4% after the program. Conclusion: Based on the findings of the present study, it can be concluded that there was a statistically significant improvement in female workers’ knowledge regarding general information about breast cancer during the posttest, as noticed in the present effect of the program. Recommendations: During significant events like Breast Cancer Awareness Month, workplace awareness programs are expanded to promote breast cancer prevention.

Background: Infertility appeared to be influenced by several PCOS comorbidities. Obesity and insulin resistance in particular were independently linked to lower pregnancy and live birth rates, as well as a higher risk of abortion. Aim: investigate the effect of physical activity habit modifications on gynecological features among studied infertile overweight and polycystic ovary syndrome. Subjects and Methods: A quasi-experimental design at Beni-Suef University Hospital's gynecological and infertility clinics; a purposive sample of 116 women with polycystic ovary syndrome, overweight, and obesity, was selected. Tools: I: The Arabic-structured interview questionnaire contains personal data; II: Block Adult Physical Activity (PA) Screener was the predictor for physical activity. This tool assessed the frequency and duration of job-related, daily life, and leisure activities. Results: reveals that 87.5% of Mild Physical Activity of the study group women has duration of menstrual cycle of less than 3 days (hypomenorrhea before intervention. Compared to 68% of moderate physical activity had a normal duration of the menstrual cycle of 3-5 days after the intervention. About 93.6%, of Mild Physical Activity (sedentary lifestyle) of the study group women have an irregular cycle before intervention. Compared to 80% of moderate physical activity had a regular cycle after the intervention. Conclusion: Physical activity habit modifications affect gynecological features among studied infertile overweight and polycystic ovary syndrome Recommendations: A study can be conducted to assess the PCOS on pregnancy outcomes and childbirth.

Background: Abdominal examination is crucial in the initial assessment of labor, helping to confirm its onset and assess fetal condition, including lie, presentation, and position. The Leopold maneuvers comprise four steps: identifying fetal lie and presentation, locating the fetal spine, assessing engagement of the presenting part, and confirming fetal position by locating the forehead and occiput in the lower abdomen. Aim: evaluate how an instructional package influenced nursing practices in general procedures, abdominal examination, palpation, and auscultation of fetal heart rate during the first stage of normal labor. Subjects and Methods: Design: A quasi-experimental design with a one group pre-posttest. Setting: labor unit at Beni-Suef University and Beni-Suef Specialist Hospitals. Sample: A convenience sample consisting of 50 maternity nurses. Tools: Observational checklist: Part I: checklist used to assess the general practices. Part II: checklist of performing abdominal examination. Part III: The checklist of performing Leopold's maneuvers. Part IV: The checklist of performing auscultation fetal heart rate. Results: 30% of nurses filled out admission card information pre-instruction compared to 80 post-one. Also, 40% of the nurses had position women in a dorso-recumbent position pre-instruction while 80% were done post-one, p value ≤0.001 for all sub item of abdominal examination. Moreover, 68.0% of nurses did not auscultate fetal heart rate pre-instruction compared to 12.96% post-one. Moreover, it shows that there were highly statistically significant differences for all items of the studied maternity nurses’ reported practices regarding performing Leopold's maneuver items at p-value ≤0.001 between pre- and post-instructional package implementation. Conclusion: There was a significant improvement in maternity nurses' practices concerning nursing care in the first stage of normal labor, covering general practices, fetal heart rate auscultation, abdominal palpation, and post-instruction examination. Recommendations: Implementing a periodic in-service training program aims to enhance maternity nurses' practices for abdominal and vaginal examination, and auscultation of fetal heart rate.

This study aimed to evaluate the effectiveness and safety of retrograde intrarenal surgery (RIRS) compared with mini-percutaneous nephrolithotomy (mini-PCNL) in children with renal pelvic stones < 2 cm and density > 1000 HU. A prospective randomized clinical trial was conducted at Beni-Suef University Hospitals including 70 children aged 3–14 years. Eligible participants had a single renal pelvic stone < 2 cm with density > 1000 HU on low-dose non-contrast CT and were randomized equally to undergo RIRS or mini-PCNL (n = 35 each). The stone-free rates at one month were 91.4% for RIRS and 94.3% for mini-PCNL (p = 0.643). RIRS was associated with shorter operative time (72.7 vs. 80.5 min), reduced hospital stay (1.1 vs. 1.3 days), shorter catheterization (1.2 vs. 2.1 days), and lower radiation exposure (34.6 vs. 115.1 s) (all p < 0.05). Hemoglobin drop was minimal and not significant in RIRS (0.01 g/dL; p = 0.097) and greater in mini-PCNL (0.74 g/dL; p < 0.001). Complication rates were similar (11.4% vs. 17.1%; p = 0.550). Both RIRS and mini-PCNL are effective and safe for managing pediatric renal stones < 2 cm with high density. RIRS offers comparable efficacy with less invasiveness, shorter recovery, and lower radiation exposure. Mini-PCNL remains a valuable option in anatomically complex or lower-calyceal cases.

Background: Pregnancy loss and abortion are psychologically impactful events leading to pain, hospitalization, social limitations, and lifestyle changes. Societal responses to abortion are influenced by religious, cultural, and psychosocial factors, and a woman’s comprehension of abortion affects her access to safe reproductive health services. Post-abortion care counseling, utilizing frameworks like PLISSIT, assists women in making informed decisions by addressing psychological, emotional, and social complications, thereby enhancing their well-being and adaptation. Aim: The current study was conducted to evaluate women’s general knowledge regarding abortion after implementation counseling based on PLISSIT model. Subjects and Methods: Design: A quasi-experimental (pre- and post-test) research design was used. Subjects &amp; Settings: A convenient sample of 92 women who had abortions affiliated obstetrics &amp; gynecology unit at Beni-Suef University Hospital. Tools: (1) structured interview questionnaire. (2) women’s knowledge regarding abortion and management. (3) post-abortion counseling based on the PLISSIT model. Results: the mean age 28.13±5.824 years, 87% were married and 42.4% had intermediate education. Results proved satisfactory answers were among women’s aged 20-30 years. It revealed that 7.6% of the studied women pre-counseling improved to 54.3% satisfactory answer post implementation of counseling sessions. Moreover, 7.6% of studied women who married had satisfactory answer knowledge before the counseling which improved into 69.5% satisfactory answer post implementation of counseling sessions. Conclusion: Based on the results of the current study, it was observed that there was no There was no statistically significant relation between the studied women’ total knowledge level and their age, marital status, and educational level. However, post counselling; satisfactory answers were more proven among young, married women who had intermediate education. Recommendations: Implement an educational counseling program based on PLISSIT and provide model for nurses to improve their knowledge to improve women’s knowledge, so, they will be able to counseling for women after that.

Use of ChatGPT in physical therapy education: Comparative study of usefulness to students and ease of use perceptions, and descriptions of usage patterns.

Background: Drug-induced cardiovascular adverse drug reactions, including QT/QTc prolongation, conduction abnormalities, and arrhythmias, remain one of the major global safety challenges. Despite its simplicity and diagnostic value, the electrocardiogram is underutilized in real-world pharmacovigilance, particularly in outpatient and resource-limited settings. This review assesses the contribution of ECG monitoring to the detection of cardiac ADRs across major therapeutic classes. Methods: This systematic review was conducted within the PECO framework. Detailed searches across MEDLINE/PubMed, EMBASE, Web of Science, Scopus, and grey literature sources were conducted. Eligible studies were trials involving human subjects receiving medications, with cardiac outcomes documented by ECG. Data extractions included ECG parameters related to the study drugs, demographic data, and clinical outcomes. Results: In studies representing more than 1.7 million patients, ECG-detected cardiac ADRs occurred in about 1.06% of the exposed, with higher frequencies among psychotropic (1.8%) and chemotherapeutic agents (1.6%). The most frequent abnormality was QT/QTc prolongation, followed by conduction delays and arrhythmias. Automated EHR-based systems (NLP+RDI) showed high performance: sensitivity 93.8%, specificity 91.8%, and a reduction in manual review workload of approximately 75%. Demographic and clinical risk factors consistently identified as associated with higher ADR risk included older age, male sex, polypharmacy, and pre-existing cardiovascular disease. Conclusion: ECG-based pharmacovigilance represents a robust and scalable approach toward cardiac ADR detection across diverse drug classes. Routine ECG monitoring, integrated with automated EHR-driven detection, offers a more sensitive, timely, and efficient approach to identifying ADRs, particularly in real-world, polypharmacy settings. Peer Review History: Received 26 September 2025; Reviewed 10 November 2025; Accepted 22 December; Available online 15 January 2026 Academic Editor: Dr. Gehan Fawzy Abdel Raoof Kandeel, Pharmacognosy Department, National Research Centre, Dokki, 12622, Giza, Egypt, gehankandeel9@yahoo.com Reviewers: Dr. Tamer Elhabibi, Suez Canal University, Egypt, tamer_hassan@pharm.suez.edu.eg Dr. Sameh Abdelmoneem Mohammed Ali, Faculty of Pharmacy, Beni-Suef University, Egypt, same7_pharma18@yahoo.com

Pre-eclampsia and its related consequences last to be significant factors in maternal morbidity and mortality, particularly in high-risk pregnant women. Aspirin has revealed promise in reducing these risks, particularly when initiated early in gestation. This study evaluated the effectiveness of 75 mg aspirin, started before 12 weeks gestation, in reducing adverse pregnancy outcomes among high-risk women. We achieved a randomized controlled experiment with high-risk pregnant women. Pregnant women were randomly allocated to receive either 75 mg of Aspirin or a placebo. Daily from the point of enrolment. (< 12 weeks gestation) until 36 weeks of gestation or until delivery. Established clinical criteria defined high-risk status. The primary outcome was the pre-eclampsia occurrence. The secondary outcomes encompassed neonatal intensive care unit (NICU) admission, preterm birth, fetal growth restriction (FGR), perinatal mortality, neonatal morbidity, gestational hypertension, and postpartum hemorrhage. Outcomes were analyzed utilizing Chi-square, two-sample z-tests, and Fisher’s exact test. This study was approved by the Ethical Committee of the Faculty of Medicine, Beni-Suef University, with approval number FWA00015574. The trial was first registered on ClinicalTrials.gov (Registration number: NCT07087067) on 25/07/2025. The occurrence of pre-eclampsia was markedly reduced in the aspirin group (8.9%) relative to the control group (28.6%) (p = 0.026). Aspirin also significantly reduced rates of FGR (4.4% vs. 19.0%, p = 0.045), NICU admission (8.9% vs. 28.6%, p = 0.026), and composite neonatal morbidity (8.9% vs. 31.0%, p = 0.014). Differences in preterm birth (2.2% vs. 9.5%, p = 0.19) and perinatal death (0% vs. 2.4%, p = 0.48) did not reach statistical significance. No increase in postpartum hemorrhage or maternal complications was noted with aspirin use. Early beginning of 75 mg low-dose aspirin in high-risk pregnant women significantly reduced pre-eclampsia and several adverse neonatal outcomes without increasing maternal risk. These findings support that early initiation of low-dose Aspirin is a secure and efficacious approach for the prevention of pre-eclampsia in pregnant women at elevated risk.

Background: Autoimmune diseases like systemic lupus erythematosus and multiple sclerosis increase the risk of infertility and premature ovarian failure in females. Oocyte cryopreservation (OC) is now offered in fertility clinics to women who want to preserve their reproductive potential by postponing childbearing. OC enhances reproductive autonomy, promotes social equity, and allows women additional time to achieve reproductive goals while mitigating risks of aneuploidies and birth defects associated with advanced maternal age. Aim: The current study was conducted to evaluate women's attitude regarding oocyte cryopreservation after an educational program implication. Subjects and Methods: Design: A quasi-experimental design was used. Sample and Settings: A study from Beni-Suef University on a purposive sample consisted of 334 working females at Beni-Suef University. Tools: The current investigation was conducted using two data gathering instruments: (I): A structured interviewing questionnaire; (II): Perceptions of oocyte cryopreservation among women. Results: The study reveals that 60.2% of working females aged 20-30 are single, 60.5% are single, and 59.3% are rural residents. 48.8% have insufficient income. Post-program implementation, attitudes towards oocyte preservation presented a significant difference, with a mean score of 48.52±4.66, compared to 36.83±4.87 pre-program. The study also found a significant relationship between age, marital status, and educational level, but not post-program. Conclusion: Based on the findings of the present study, it can be concluded that after program implementation, the studied female workers at Beni-Suef University attained a positive attitude regarding oocyte cryopreservation. Recommendations: Establishing a health educational program for oocyte cryopreservation technology for nursing students.

Perception of corneal extraction from cadavers among Beni-Suef University Hospital Physicians, Egypt

Background: The arteriovenous fistula is recognized as the ideal and most commonly utilized technique of vascular access for hemodialysis, typically regarded as the optimum standard in clinical practice. Instructional programs are imperative for equipping nurses with the required skills to enhance patient outcomes and decrease overall health care expenses. Objectives: The aim of the study was to evaluate the effect of instructional guidelines on nurses' performance regarding care of arteriovenous fistula puncture for hemodialysis children. Methods: The study design was quasi-experimental. At Beni-Suef University Hospital's pediatric hemodialysis unit, the study was carried out. A purposive sample composed of 50 nurses. Data were gathered utilizing two tools: The 1st tool is the interviewing questionnaire sheet. The 2nd tool is observational checklists. Results: More than three-quarters of nurses had an unsatisfactory total level of knowledge, and the majority of them had an incompetent total level of practice regarding arteriovenous fistula puncture care in the pre-instructional guidelines implementation. While most of the nurses had a satisfactory total level of knowledge, four-fifths of them had a competent total level of practice in the post-instructional guidelines' implementation with a highly statistically significant difference (p-value &lt; 0.000). Also, there was a highly statistically significant positive correlation between nurses' total knowledge and total practice level pre- and post-implementation. Conclusion: The study findings confirmed the effectiveness of the instructional guidelines in improving nurses' knowledge and practices concerning arteriovenous fistula puncture care for hemodialysis children. Recommendation: Implementing periodical instructional programs for nurses concerning arteriovenous fistula puncture care is necessary to update and improve their performance.

Assessment of performance of disc diffusion, Minimum inhibitory concentration gradient tests and Vitek 2 device for ceftazidime/avibactam susceptibility testing of pseudomonas Species in Beni-Suef University Hospital