Hamburgers Research Articles

Antioxidant and Preservative Effects of Epilobium angustifolium Extract in Beef Burgers Products: Physicochemical Properties, Color Stability, Lipid Oxidation, and Molecular Docking Analyses.

Consumers are increasingly seeking natural alternatives to synthetic preservatives in meat products. This study evaluated the effects of willow E. angustifolium extract on beef burgers' physicochemical properties, color stability, and lipid oxidation during refrigerated storage. The extract demonstrated significant antioxidant activity, with a total phenolic content of 1263.48 mg GAE/L and a total flavonoid content of 278.43 mg ce/L. The pH values of the beef burgers decreased over time, with significant effects on Days 4 and 8 in the treated groups, except T1. The color properties, including lightness (L*), redness (a*), and yellowness (b*), were affected by the plant extract, with 1 g and 3 g concentrations leading to darker and redder hues. Moreover, adding 9 g extract led to discoloration due to increased b* values. Thiobarbituric acid-reactive substances analysis showed increased lipid oxidation, with the T3 group (9 g extract) exhibiting higher values, suggesting a prooxidant effect at higher concentrations. Gas chromatography-mass spectrometry analysis identified a range of phytochemicals, including α-pinene and β-thujone, which contributed to the complex, volatile profile of the extract. High-performance liquid chromatography analysis revealed the presence of significant phenolic compounds, such as ascorbic acid and gallic acid, with high antioxidant potential. Molecular docking studies indicated that gallic acid exhibited a moderate binding affinity with the target protein 9R-lipoxygenase (5EK8), followed by β-thujone and α-pinene, suggesting their potential as bioactive compounds in food preservation. The results provide insights into the functional potential of E. angustifolium extract as a natural antioxidant and preservative in meat products.

Bioactive chitosan based coating incorporated with essential oil to inactivate foodborne pathogen microorganisms and improve quality parameters of beef burger.

The aim of this study is to assess the impacts of chitosan (CH) coating with oregano essential oil (OEO) and thyme essential oil (TEO) (0.5%-1.0%; v/w) on the foodborne pathogens and physicochemical parameters of beef burger during refrigerated storage. Preliminary experiment (in vitro) demonstrated that 0.5% OEO and TEO were inhibited all or some of S. aureus, S. Typhimurium, and E. coli O157:H7. On day 30, the E. coli O157:H7 of burger coated with CH + OEO and TEO (1%; w/v) declined by 4 and 5 log10 CFU g-1, respectively, S. Typhimurium and S. aureus decreases (4,5-6 log10 CFU g-1) when compared to the control sample. The quality parameters of beef burger were also enhanced after the coating treatment of CH and essential oils (EOs), including pH value, TBARS, and TVB-N in burger during storage (4 °C/30 d). Besides, CH + EOs coating also reduced the deterioration of the sensory attributes of beef burger, including color, odor, and overall acceptability. The chitosan coatings with EOs have superior mechanical qualities than the control sample, also, the structure of the films was evaluated by SEM. In conclusion, CH coating with EOs (OEO, ETO; 1%) regarded as a successful strategy to improve the quality and prolong the shelf life of beef burger.

Use of propidium monoazide coupled with real-time PCR methodology to evaluate Escherichia coli O157 lethality in beef burgers after organic acid addition.

In order to achieve bacterial inactivation in beef burgers, one of the alternatives proposed is to add organic acids as part of their formulation. The aim of this work was to evaluate the effectiveness of propidium monoazide coupled to qPCR (PMA-qPCR) as counting methodology to assess the addition of lactic acid (LA), caprylic acid, and fumaric acid (FA) alone and combined as inactivation agents on Escherichia coli O157 (both non-toxigenic and Shiga toxin-producing E. coli-[STEC]-strains). The effects on physicochemical properties were also evaluated. PMA-qPCR targeting stx2 and uidA genes allows quantifying STEC and non-toxigenic E. coli O157. The most effective acid combination was LA+FA for non-toxigenic E. coli O157 with a reduction of 1.06logCFU/g measured by PMA-qPCR. However, this treatment increased hardness and chewiness once the beef burgers were cooked. Moreover, cooking weight loss was negatively affected by the addition of all organic acids tested. PMA-qPCR methodology permits to detect and quantify viable but nonculturable E. coli O157 in the beef burgers, a common limitation encountered in the traditional plate count method. The presence of bacteria in this metabolic state could explain differences among the values obtained by both methodologies. Addition of organic acids to beef burger formulations was not as effective as expected to inactivate E. coli.

Changes in Quality Features of Pork Burgers Prepared with Chokeberry Pomace During Storage

This study aimed to evaluate the effect of adding shredded black chokeberry (Aronia melanocarpa) pomace on the quality of heat-treated and vacuum-packed pork burgers stored for 14 days at +4 °C. Four burger treatments—Control (BC) and products with 2%, 3.5%, and 5% chokeberry pomace (B2, B3.5, and B5, respectively)—were analyzed for physicochemical properties (thermal loss, shrinkage, content of selected chemical components, pH, color parameters, and shear force) and microbial quality (aerobic mesophilic microorganisms, psychrotrophic bacteria, lactic acid bacteria, Enterobacteriaceae, Pseudomonas spp., Brochothrix thermosphacta, and yeasts and molds). The addition of chokeberry pomace increased (p < 0.05) the thermal loss of pork burgers from 23.5% (BC) to 30.8% (B5) and decreased (p < 0.05) the pH from 6.93 (BC, day 1) to 6.74 (B5, day 14). The introduction of pomace into the pork burgers also significantly (p < 0.05) affected the content of chemical components. However, the nutritional value of pork burgers remained high, with a protein content not lower than 26.68% (BC) and a fat content not exceeding 13.96% (B5). The most affected quality feature of the pork burgers was color. Products B2, B3.5, and B5 exhibited lower L* and b* parameters (p < 0.05) while showing higher a* values. The b* parameter had negative values for products B3.5 and B5 on days 7 and 14. The use of chokeberry pomace did not deteriorate the microbial quality of pork burgers, as indicated by the maximum total count of aerobic mesophilic microorganisms, which reached 4.4 × 103 cfu/g (B3.5). Moreover, on the final day of storage, moderate antimicrobial properties of chokeberry pomace were observed, with a lower (p < 0.05) number of lactic acid bacteria and Pseudomonas spp. in products B2–B5 compared to BC. The results indicate that incorporating shredded chokeberry pomace into burger-type ground pork products does not present major technological difficulties. However, raw shredded fruit pomace is a perishable microbiological material and requires rapid processing. Further research on the use of chokeberry pomace in burger-type meat products is recommended due to its nutritional value and health-promoting properties. However, this research should include a comprehensive sensory evaluation of the finished product.

Effect of Extrusion Conditions on the Characteristics of Texturized Vegetable Protein from a Faba Bean Protein Mix and Its Application in Vegan and Hybrid Burgers.

The aim of this study was to produce texturized vegetable proteins (TVPs) from faba bean protein via low-moisture extrusion. The effect of extrusion variables including temperature (110, 125, and 140 °C at the die), feed moisture content (30, 35, and 40%), and screw speed (200, 300, and 400 rpm) on the TVP properties were investigated. An increase in feed moisture content or extruder temperature reduced the specific mechanical energy and torque by 40-45% during extrusion. An increase in feed moisture created TVPs with lower bulk densities and rehydration ratios while an increase in extruder temperature or screw speed increased the bulk density of the TVPs. An increase in screw speed also caused a decrease in the water holding capacity of the milled TVP flours. The TVP flours had a 33-70% higher oil holding capacity than the raw material. The texture profile showed that an increase in feed moisture influenced TVP hardness, gumminess, and chewiness with higher values compared to the treatments with lower moisture contents. Springiness, cohesiveness, and resilience were more affected by a change in screw speed with higher values at 200 rpm. The best parameters were selected (125 °C, 40% MC, 300 rpm) to produce TVP to use as a partial (hybrid burger) and complete (vegan burger) replacement of beef in a burger patty. The replacement of 25% beef with TVPs in a hybrid burger increased the cooking yield and moisture retention and decreased the thickness and diameter change compared to the beef burger without TVPs. In a vegan formulation, the faba bean TVP burger had lower cooking yield and moisture retention than commercial products.

Reduction of polycyclic aromatic hydrocarbons in pork burgers using high-pressure processed white grape pomace

Reduction of polycyclic aromatic hydrocarbons in pork burgers using high-pressure processed white grape pomace

Hazelnut skin polyphenolic green extract as a promising natural antioxidant in pork burgers: Assessment of quality parameters and consumer acceptance.

Hazelnut skin polyphenolic green extract as a promising natural antioxidant in pork burgers: Assessment of quality parameters and consumer acceptance.

Effect of Khistawi Date Kernel Powder on Some Sensory, Chemical and Biological Characteristics of Beef Burger

Abstract This study was completed with the assistance of the Food Sciences Department Laboratory at the Faculty of Agriculture in Anbar. The kernels of Khistawi were chosen because there are few studies on it, Khistawi dates are widely consumed in Iraq, and a fair proportion of the kernels are not exploited The good quality Khistawi dates were selected and the poor ones were excluded to avoid differences between the same type and to ensure a safe crop with valuable nutritional components, then the kernel were Isolated, cleaned, dried, and finely crushed, The basic components of the seed powder were tested and the percentages were high, The amount of phenols in the alcoholic and aqueous extracts was tested and was 2759 mg/100 and 1817 mg/100 respectively. The powder was added to the burger in three proportions: 2%, 4% and 6%, With the addition of the kernel powder replaced by wheat flour, and some chemical, biological and sensory tests were worked through four periods (0,3, 7, 10 days) of cold storage at degree 4°C. The cooking yield in the control sample after 10 days of storage was 69.10% compared to the last treatment sample in which the cooking yield was 76.45%. As for the sensory evaluation, the evaluators’ impressions gave a clear picture, in the scores given to the attributes at the level (P≤0.05*) between samples. Flavor, juiciness and overall acceptability qualities maintained a high level of acceptability and evaluation throughout storage periods and under a high level of morale followed by texture and color. We conclude that there is a clear preference for samples with 4%.

Nutritional properties, lipid oxidation, microbial growth, and sensory evaluation of beef burgers enriched with garden cress seeds powder

Nutritional properties, lipid oxidation, microbial growth, and sensory evaluation of beef burgers enriched with garden cress seeds powder

Faba bean protein reduces lipid oxidation and changes physicochemical quality traits of hybrid beef burgers stored in high oxygen, and high nitrogen, modified atmosphere packaging

Faba bean protein reduces lipid oxidation and changes physicochemical quality traits of hybrid beef burgers stored in high oxygen, and high nitrogen, modified atmosphere packaging

Development of anti-bacterial bio-transfer double sheet layer of polyvinyl alcohol/carboxymethyl cellulose films infused with Astragalus tribuloides leaf extract for beef burgers preservation

Development of anti-bacterial bio-transfer double sheet layer of polyvinyl alcohol/carboxymethyl cellulose films infused with Astragalus tribuloides leaf extract for beef burgers preservation

Effects of Chitosan-based Coating Enriched with Peanut (Arachis hypogaea) Skin Extract on Physicochemical, Microbiological and Sensory Characteristics of Beef Burger During Cold Storage

The current study aimed to create a chitosan-based coating enriched with peanut skin extract (PSE) at a concentration of 0.5, 1 and 1.5%, and evaluate its impact on the physicochemical, microbiological and sensory characteristics of fresh beef burger stored at 4±1°C for 15 days. All coated burgers had significantly higher L*, a* and b* values than the uncoated control during storage. Also, the results showed that application of chitosan coating enriched with 0.5, 1 and 1.5% PSE significantly increased total phenolic content (TPC) and antioxidant activity (AA), inhibited lipid oxidation as evaluated by thiobarbituric acid reactive substances (TBARS) and peroxide value (PV), retarded microbial growth and enhanced sensory characteristics of burger samples. Moreover, the positive effects of PSE on all investigated quality characteristics were proportional with the extract concentration. Therefore, using chitosan-based coating enriched with PSE enhances the stability of beef burger during cold storage and can be utilized in the meat industry.

Challenges in the use of natural flavorings and labeling compliance in meat preparations in the Umbria region (central Italy).

There is an increasing trend among food business operators to use natural flavorings in meat preparation to reduce microbial spoilage and extend the shelf life. The objective of this study was to evaluate the prevalence of the use of natural flavoring mixtures in meat preparation, the possible presence of additives in natural flavorings, and the assessment of any transfer of additives contained in natural flavorings into experimentally prepared beef burgers. Based on a preliminary survey conducted as part of the study, approximately 87% of establishments used natural flavoring mixtures in meat preparation. The same natural mixtures used by local industries were added to minced meat in order to produce experimental hamburgers. Citric acid was found in both natural flavoring mixtures ranging from 1760 mg/kg to 92,731 mg/kg and experimental burgers ranging from 57 mg/kg to 2248 mg/kg. Ascorbic acid was present in all natural flavoring mixtures from 260 mg/kg to 98,858 mg/kg but was recovered in three burgers up to 1075 mg/kg. Acetic acid was found in three flavoring mixtures (range 23,539-77,421 mg/kg) and transferred to three treated burgers (range 3063-3202 mg/kg). Nitrite was never found in hamburgers and only once in a natural flavoring mixture at a dose of 26 mg/kg, whereas nitrate was found in some natural flavoring mixtures up to 788 mg/kg but never in hamburgers. The study has raised some critical issues about the appropriate and conscious use of natural flavorings and the possible inadequate meat preparation labeling in the Umbria region.

Antioxidant and Nutritional Potential of Artichoke (Cynara scolymus L.) By-Product Extracts in Fat-Replaced Beef Burgers with Hydrogel Emulsions from Olive Oil

Beef burger consumption has been questioned due to the burgers’ high levels of saturated fatty acids and the use of synthetic additives for preservation. In order to improve the acceptability and health benefits of this product, two functional ingredients, artichoke and extra-virgin olive oil (EVOO), have been used to formulate four different beef burgers: a control burger (CB), a fat-replaced burger with an EVOO emulsion (FRB), and two FRB formulations with the incorporation of enzymatically treated (FRB-TAE) or untreated (FRB-AE) artichoke extract. Fat replacement significantly affected the lipid profile of the burgers, increasing oleic acid levels and n-3 PUFA such as α-linolenic and eicosapentaenoic acids. Artichoke incorporation exerted beneficial effects on the antioxidant activity, as well as on the total phenolic content of the reformulated burgers, leading to a decrease in color changes, lipid and protein oxidation after 3 days of storage at 4 °C, as well as reducing the formation of volatile compounds such as hexanal, 2,3-Octanedione, or 1-Octen-3-ol. No differences were found between formulations for the sensory parameters studied. These results demonstrate a possible revalorization of artichoke by-products by improving the nutritional properties of beef burgers, revealing a potential application as a higher value-added ingredient in the meat industry.

Plant-Based Ingredients Utilized as Fat Replacers and Natural Antimicrobial Agents in Beef Burgers.

The present study aimed to find solutions based on the use of plant-based ingredients that would improve the nutritional quality of meat products as well as ensure sensory and microbiological quality. Two fat replacers, lemon albedo (Citrus lemon) and carob seed gum (Ceratonia siliqua), were investigated by chemical analysis and panel testing to evaluate their effect on the nutritional and sensory quality of beef burgers. The antimicrobial activity of two plant extracts, from nettle (Urtica dioica) leaves and medlar (Eriobotrya japonica) seeds, was studied, evaluating the intensity of inhibitory action and the minimum inhibitory concentration against Pseudomonas spp. and Listeria innocua strains by plate test. In addition, the antioxidant activity of both extracts was evaluated. Based on the results, lemon albedo and medlar seed extracts were validated in a food model (beef burger) by a storage test and a challenge test. The storage test results highlight that medlar seed extract prevents the formation of thiobarbituric acid reactive substances (TBARSs) and ensures microbiological quality, inhibiting Enterobacteriaceae and Pseudomonas spp. Anti-Listeria efficacy was confirmed in situ by challenge test results. In conclusion, although fat replacers ensure nutritional and sensory quality, they do not satisfy microbiological quality. This study clearly demonstrates that the safety of low-fat burgers can only be achieved through the combination of appropriate fat replacers with well-selected natural antimicrobial extracts.

Synergistic effects evaluation of jabuticaba and strawberry extracts on oxidative stability of pork burgers

Synergistic effects evaluation of jabuticaba and strawberry extracts on oxidative stability of pork burgers

Physicochemical, Antioxidant, Organoleptic, and Anti-Diabetic Properties of Innovative Beef Burgers Enriched with Juices of Açaí (Euterpe oleracea Mart.) and Sea Buckthorn (Hippophae rhamnoides L.) Berries

Background: The aim of this study was to evaluate the selected quality parameters of innovative beef burgers produced with the addition of açaí and/or sea buckthorn berry juices. Methods: Five variants of innovative burgers were obtained, differing in the proportion of juices in the recipe. The pH of meat stuffing, thermal losses, production yield, color (CIE L*a*b*), content of polyphenolic compounds, degree of oxidation of the lipid fraction (TBARS), and antioxidant activity against ABTS radicals were determined. Anti-diabetic activity was measured as the ability to inhibit α-glucosidase and dipeptidyl peptidase−4 activity. A sensory evaluation was also performed. Results: Beef burgers formulated with açaí and sea buckthorn juices had up to five times higher total polyphenol content than burgers without added juices. The addition of the juices increased antioxidant activity against ABTS radicals (from 42 to 440 µmol/L/100 g) and effectively inhibited oxidation of the lipid fraction of the beef burgers. Recipe modifications resulted in changes in the color parameters of the beef burgers and had a positive effect on the sensory quality attributes evaluated. Beef burgers containing 0.5 g of açaí juice and 1.0 g of sea buckthorn juice were rated the best in terms of acceptability of appearance, aroma, color, juiciness, and tenderness. The addition of açaí and sea buckthorn juice did not increase the inhibitory activity against α-glucosidase and dipeptidyl peptidase-IV of the innovative beef burgers. Conclusions: The proposed recipe modification may be an effective way to fortify beef burgers with phytochemicals with antioxidant properties while maintaining their sensory properties.

Effect of Incorporating Grape Seeds as a Natural Preservative on the Properties of Beef Burgers During Freeze Preservation

Effect of Incorporating Grape Seeds as a Natural Preservative on the Properties of Beef Burgers During Freeze Preservation

Antimicrobial Properties of Fennel By-Product Extracts and Their Potential Applications in Meat Products.

Background: Beef burgers are perishable meat products, and to extend their shelf life, EU Regulation 1129/11 permits the use of certain additives. Objectives: However, given the concerns of health-conscious consumers and the potential toxicity of synthetic substances, this study aimed to explore the use of fennel waste extracts as natural preservatives. Methods: This study characterized the bioactive compounds (phenolic content), the antioxidant activity (ABTS+ and DPPH assay), and the antimicrobial properties (against Salmonella enterica serotype Enteritidis, Escherichia coli, Staphylococcus aureus, Bacillus cereusi, and Pseudomonas aeruginosa) of different fennel waste extracts (LF, liquid fraction; SF, solid fraction and PF, pellet fraction). Additionally, the potential use of the best fennel extract was evaluated for its impact on beef burger shelf life (up to 18 days at 4 ± 1 °C) in terms of microbiological profile, pH, and activity water (aw). Results: The PF extract, which was rich in flavones, hydroxybenzoic, and hydroxycinnamic acids, demonstrated the highest antioxidant and antimicrobial activities. Microbiological analyses on beef burgers with PF identified this extract as a potential antimicrobial substance. The aw and pH values did not appear to be affected. Conclusions: In conclusion, fennel extracts could be proposed as natural compounds exploitable in beef burgers to preserve their quality and extend their shelf-life.

Monoammonium Glutamate: What Is Its Potential in the Reduction of Sodium Content in Different Food Matrices?

ABSTRACTThe objective of the study was to assess the efficacy of monoammonium glutamate (MAG) in reducing the sodium chloride (NaCl) content (CCRD) of food matrices, including shoestring potatoes, requeijão cheese, and beef burgers. To this end, a time intensity (TI) analysis, temporal dominance of sensations (TDSs), and acceptance tests were employed to evaluate the NaCl reduction in various food items. The TI analysis showed that all experimental units had a mean salty taste intensity that equaled or exceeded the control. The TDS profiles of shoestring potatoes and requeijão cheese and the control were similar. However, the requeijão cheese exhibited significantly lower acceptance than the controls. The food matrices influenced the potential use of MAG and the CCRD. The addition of 2% MAG to the formulation reduced the NaCl content by up to 50% in shoestring potatoes and 65% in beef burgers without compromising the temporal perception of a salty taste and sensory acceptance.