Bacterial Input Research Articles

Molecular compositional variation of organic matter deposited on the East Tasman Plateau during the Paleocene–Eocene Thermal Maximum

Molecular compositions in sediments collected by Ocean Drilling Program Leg 189 at Site 1172 in the Tasmanian Gateway, Australia have been characterised by gas chromatography–mass spectrometry to assess the impact of the Paleocene–Eocene Thermal Maximum (PETM) on organic matter origins and evolution. Total organic carbon (TOC) and sulfur contents reach the lowest values immediately before the initiation of the negative carbon isotope excursion (CIE) in the shallow marine environment on the East Tasman Plateau. The TOC content increases slightly while the sulfur content increases more substantially during deposition of the CIE, suggesting the occurrence of low-oxygen environments during the PETM event. The studied section is thermally immature, as shown by the dominance of biological configuration biomarkers such as 17β(H),21β(H)-hopanes (ββ) and hopenes. Changes in relative abundance of compound classes and isomer ratios are largely caused by the variation of source inputs and depositional conditions. Very low abundance of marine steroids during the CIE, but very enriched hopanoids, indicate low marine productivity, in accord with substantial remineralisation of organic matter in the bottom waters. The ββ-hopanes are the most abundant hopanoid series, followed by the neohop-13(18)-ene and hop-17(21)-ene series, further verifying the immaturity of the organic constituents in the samples. Hopane ratios (ββ/(βα + αβ) and βα/(βα + αβ) with different carbon numbers are systematically higher in sediments deposited during the CIE than those formed pre- and post-CIE, suggest increased bacterial inputs. Higher C30/C29 and C30/C31 hopanoid ratios in the CIE sediments are consistent with a greater contribution from in situ bacterial organic matter, rather than continental soil erosion by flooding. This is supported by the higher C27–32 hopanoids/(C27 + C29 + C31n-alkanes) ratio and sulfur content during the CIE. A relatively lower TOC content in the sediments formed during the PETM is inferred to have been caused by dilution by both enhanced detrital input and intensified bacterial consumption. This study demonstrates that the biomarker approach can play an important role in the identification of organic matter origin and characterisation of depositional environment for PETM sequences.

Mosquitoes reared in distinct insectaries within an institution in close spatial proximity possess significantly divergent microbiomes.

The microbiome affects important aspects of mosquito biology and differences in microbial composition can affect the outcomes of laboratory studies. To determine how the biotic and abiotic conditions in an insectary affect the composition of the bacterial microbiome of mosquitoes we reared mosquitoes from a single cohort of eggs from one genetically homogeneous inbred Aedes aegypti colony, which were split into three batches, and transferred to each of three different insectaries located within the Liverpool School of Tropical Medicine. Using three replicate trays per insectary, we assessed and compared the bacterial microbiome composition as mosquitoes developed from these eggs. We also characterised the microbiome of the mosquitoes' food sources, measured environmental conditions over time in each climate-controlled insectary, and recorded development and survival of mosquitoes. While mosquito development was overall similar between all three insectaries, we saw differences in microbiome composition between mosquitoes from each insectary. Furthermore, bacterial input via food sources, potentially followed by selective pressure of temperature stability and range, did affect the microbiome composition. At both adult and larval stages, specific members of the mosquito microbiome were associated with particular insectaries; and the insectary with less stable and cooler conditions resulted in slower pupation rate and higher diversity of the larval microbiome. Tray and cage effects were also seen in all insectaries, with different bacterial taxa implicated between insectaries. These results highlight the necessity of considering the variability and effects of different microbiome composition even in experiments carried out in a laboratory environment starting with eggs from one batch; and highlights the impact of even minor inconsistencies in rearing conditions due to variation of temperature and humidity.

Mycobacterium tuberculosis SecA2-dependent activation of host Rig-I/MAVs signaling is not conserved in Mycobacterium marinum.

Retinoic acid inducible gene I (Rig-I) is a cytosolic pattern recognition receptor canonically described for its important role in sensing viral RNAs. Increasingly, bacterially-derived RNA from intracellular bacteria such as Mycobacterium tuberculosis, have been shown to activate the same host Rig-I/Mitochondrial antiviral sensing protein (MAVS) signaling pathway to drive a type-I interferon response that contributes to bacterial pathogenesis in vivo. In M. tuberculosis, this response is mediated by the protein secretion system SecA2, but little is known about whether this process is conserved in other pathogenic mycobacteria or the mechanism by which these nucleic acids gain access to the host cytoplasm. Because the M. tuberculosis and M. marinum SecA2 protein secretion systems share a high degree of genetic and functional conservation, we hypothesized that Rig-I/MAVS activation and subsequent induction of IFN-β secretion by host macrophages will also be conserved between these two mycobacterial species. To test this, we generated a ΔsecA2 M. marinum strain along with complementation strains expressing either the M. marinum or M. tuberculosis secA2 genes. Our results suggest that the ΔsecA2 strain has a growth defect in vitro but not in host macrophages. These intracellular growth curves also suggested that the calculation applied to estimate the number of bacteria added to macrophage monolayers in infection assays underestimates bacterial inputs for the ΔsecA2 strain. Therefore, to better examine secreted IFN-β levels when bacterial infection levels are equal across strains we plated bacterial CFUs at 2hpi alongside our ELISA based infections. This enabled us to normalize secreted levels of IFN-β to a standard number of bacteria. Applying this approach to both WT and MAVS-/- bone marrow derived macrophages we observed equal or higher levels of secreted IFN-β from macrophages infected with the ΔsecA2 M. marinum strain as compared to WT. Together our findings suggest that activation of host Rig-I/MAVS cytosolic sensors and subsequent induction of IFN-β response in a SecA2-dependent manner is not conserved in M. marinum under the conditions tested.

Organic Geochemical Characteristics of Ngrayong Formation Polaman Sediment Rock, Northeast Java Basin-Indonesia

A study of the sedimentary rocks of the Ngrayong formation has been carried out on five samples from the Polaman outcrop point to determine the potential of coal as a source rock for producing oil and gas through GC-MS analysis. Biomarker analysis shows the presence of n-alkanes (C16-C36) with a bimodal distribution, indicating that the source of organic material in sedimentary rocks comes from bacteria, algae, and vascular plants, which is supported by several parameters such as CPI, OEP, LHCPI, wax index, ACL and AlkTerr values. This dominant source of terrigenous organic matter is also proven by the TAR value, C31/C19, C29/C17 ratio, and several aromatic compounds and their derivatives. Bacterial input as an organic source of allouchtonic sedimentary rocks is also proven by the presence of hopanoid, de-A-lupane biomarkers, and C17/C31 ratio. The oxic deposition environment is indicated by the Pr/Ph ratio. CPI and OEP parameters, C29 bb/ab ratio > 0.15 and C31 22S/(22S+22R) < 1 indicate low maturity of the sediment sample. Several parameters and the presence of biomarkers stated above conclude that Ngrayong coal as a source rock has the potential to produce oil and gas.

Aliphatic Biomarker Signatures of Crude Oil from Tarakan Subbasin, Tarakan Basin, North Kalimantan, Indonesia

Organic geochemical studies of crude oil from Pamusian Field, Tarakan Subbasin, Tarakan Basin, North Kalimantan, have been done. Biomarker aliphatic hydrocarbon fractions were identified using a combined gas chromatography-mass spectrometer (GC - MS). Identified biomarkers consist of n-alkane groups, isoprenoids, bicyclic sesquiterpenoids, and pentacyclic triterpenoids. The most abundant aliphatic hydrocarbon biomarker is pristane followed by n-C19. The existence of n-alkanes shows a homologous (n-C16- n-C30) with a unimodal distribution type. The abundance of n-C19 is higher than other n-alkanes, supported by LHCPI value of 2.03, as an indicator of organic matter derived from microbial organisms. The amount of long chain n-alkanes (n-C25- n-C30) is almost the same as medium chain n-alkanes (n-C19 - n-C24) indicating the source of organic compounds is not only from microbial organisms, but also from terrestrial higher plants. The presence of 8β (H)-drimane compounds together with homodrimane shows the presence of bacterial input on the formation of oil from organic compounds. Ratio of Pr/Ph is 3.76, ratio of drimane/homodrimane is 1.058, ratio of Pr/n-C17 is 34.41, and ratio of Ph/n-C18 is 16.02 indicating the source of organic compounds came from terrestrial higher plants deposited in the oxic environment, and disposed to increase biodegradation. The CPI value is 0.95, and the highest amount of 17α (H), 21β (H)-hopane compounds suggest that Tarakan Subbasin oil was mature, and the source of organic compounds was derived from bacteria Keywords: Tarakan Basin, organic geochemistry, aliphatic hydrocarbon fraction, CPI

Mitochondrial GTP metabolism controls reproductive aging in C. elegans

Healthy mitochondria are critical for reproduction. During aging, both reproductive fitness and mitochondrial homeostasis decline. Mitochondrial metabolism and dynamics are key factors in supporting mitochondrial homeostasis. However, how they are coupled to control reproductive health remains unclear. We report that mitochondrial GTP (mtGTP) metabolism acts through mitochondrial dynamics factors to regulate reproductive aging. We discovered that germline-only inactivation of GTP- but not ATP-specific succinyl-CoA synthetase (SCS) promotes reproductive longevity in Caenorhabditis elegans. We further identified an age-associated increase in mitochondrial clustering surrounding oocyte nuclei, which is attenuated by GTP-specific SCS inactivation. Germline-only induction of mitochondrial fission factors sufficiently promotes mitochondrial dispersion and reproductive longevity. Moreover, we discovered that bacterial inputs affect mtGTP levels and dynamics factors to modulate reproductive aging. These results demonstrate the significance of mtGTP metabolism in regulating oocyte mitochondrial homeostasis and reproductive longevity and identify mitochondrial fission induction as an effective strategy to improve reproductive health.

Optimizing Crop Production with Bacterial Inputs: Insights into Chemical Dialogue between Sphingomonas sediminicola and Pisum sativum.

The use of biological inputs is an interesting approach to optimize crop production and reduce the use of chemical inputs. Understanding the chemical communication between bacteria and plants is critical to optimizing this approach. Recently, we have shown that Sphingomonas (S.) sediminicola can improve both nitrogen supply and yield in pea. Here, we used biochemical methods and untargeted metabolomics to investigate the chemical dialog between S. sediminicola and pea. We also evaluated the metabolic capacities of S. sediminicola by metabolic profiling. Our results showed that peas release a wide range of hexoses, organic acids, and amino acids during their development, which can generally recruit and select fast-growing organisms. In the presence of S. sediminicola, a more specific pattern of these molecules took place, gradually adapting to the metabolic capabilities of the bacterium, especially for pentoses and flavonoids. In turn, S. sediminicola is able to produce several compounds involved in cell differentiation, biofilm formation, and quorum sensing to shape its environment, as well as several molecules that stimulate pea growth and plant defense mechanisms.

Environmental risk assessment for fecal contamination sources in urban and peri-urban estuaries, in Escambia and Santa Rosa counties, FL, USA.

Fecal pollution of estuaries and adjacent creeks and streams is of significant concern along the Gulf of Mexico. The prospective threat to human life and water quality impairment via fecal pollution is a substantial danger to the strength and resistance of coastline areas. Pensacola, FL, has a prosperous coastal tourism industry that is utilized for numerous other uses, such as recreational watersports and boating, seafood, and shellfish harvesting. However, the frequency and severity of fecal contamination present possible socio-economic issues, specifically financial hardships. Therefore, understanding the source, abundance, and fate of fecal microbial pollutants in aquatic systems signifies an imperative initial stage for detecting the host sources and techniques to lessen their transport from the landscape. This research aimed to quantify the fecal indicator bacteria (FIB), Escherichia coli, and perform microbiological fecal source tracking to verify if the fecal inputs are of either animal or human host origin. Surface water samples were taken from urban and peri-urban creeks for two sampling periods (February 2021 and January 2022), and IDEXX Colilert-18 (USEPA Standard Method 9223) was used for E. coli enumeration. DNA extractions were obtained from each sample, and quantitative PCR was utilized for fecal microbial source tracking (MST) to detect human, dog, ruminant, and bird host-specific Bacteroides DNA. The result indicates elevated quantities of FIB, E. coli, that surpass the threshold considered safe regarding human health. E. coli at six sites over the two sampling periods exceeded the impairment threshold, reaching as high as 866.4 MPN/100ml. Fecal source tracking identified human host fecal contamination at four of nine sites, dogs at three of nine, and birds at one site. However, those sites with sources identified via MST all had E. coli levels below impairment thresholds. No sites were determined to be positive for ruminant as a source or for the pathogen Helicobacter pylori. No canine host fecal inputs were found in January 2022, and only one site with human sewage. Our results highlight the utility of MST in assessing bacterial inputs to water bodies and the challenges.

Labelling of eicosapentaenoic acid with stable isotope 13C in the marine bacterium Shewanella marinintestina

Eicosapentaenoic acid (EPA) is an essential omega-3 polyunsaturated fatty acid that plays a critical role in marine life. It is present in several marine animals, including fish, but the primary producers of EPA are phytoplankton and specific marine bacteria. Although most of the EPA present in marine animals come from phytoplankton, the bacterial input into the marine EPA food web is still unknown. The labelling of EPA within a bacterial strain could be a viable strategy to help revealing this contribution. In this work, Shewanella marinintestina IRL 567, a marine bacterium isolated from fish guts and known to produce EPA, was labelled with the stable isotope 13C at small (250-mL shake flask), bench (2.5-L shake flask), and pilot scale (50-L stirred tank bioreactor). Growing the bacterium with 13C-acetate in the culture medium demonstrated that EPA was de-novo synthesized utilizing acetate as precursor. 13C incorporation into the EPA molecule resulted in values as high as 95.5% of the synthesized EPA being labelled in small scale, 95.9% in bench scale and 91.5% in pilot scale. This simple method to label EPA proved to be effective and therefore it could be a valuable tool to follow the fate of bacterial EPA into higher trophic levels.

Lower Jurassic in the central part of the Polish Basin - Geochemical and petrological approach

Lower Jurassic grey/black horizons composed of mudstones and claystones can be found in the Polish Basin area. These horizons are interbedded in thick sandstone packets, which to date have been mostly recognised as good geothermal reservoirs. In this paper, we examined the hydrocarbon potential of the Lower Jurassic claystones and mudstones from the central part of the Polish Basin. To properly evaluate these, we described in detail the organic matter's origin and maturity, as well as the palaeoenvironmental conditions. The collected core samples were examined by geochemical (Rock-Eval pyrolysis and GC-MS) and petrological (macerals content, vitrinite, and fusinite reflectance values) analyses. The obtained results revealed that the Lower Jurassic strata have poor hydrocarbon potential and contain mostly early-mature, terrigenous organic matter. Deposition took place under dysoxic conditions of the epicontinental sea, with the serious influence of deltaic/estuarine systems. Some samples contained mature, marine organic matter dominated mostly by bacterial input. One Sinemurian/Hettangian sample contained a high contribution of algal organic matter, which can be linked with the diatoms or diatom-related organisms as C25HBI, 24-norcholestane and 4-desmethyl-23,24-dimethyl steranes were detected. This might represent some of the oldest geochemical evidence of diatoms or diatom-related organisms. A wide spectrum of plant-derived biomarkers was marked in most of the samples, which allowed us to find traces of conifers, bryophytes, and fungi. Evidence of palaeo-wildfires was also noted. Newly introduced Pyrolytic Input Ratio (PIR) and Fire Maceral Index (FMI) were also examined. The use of PIR in samples with higher marine organic matter input indicated that PIR is not suitable for these samples, as the obtained results were overestimated. The PIR and FMI results obtained from the Lower Jurassic samples dominated by terrigenous organic matter suggested that both indicators gave proper results, as well as those samples with lesser paleo-wildfire product input.

The Effects of a Typhoon on the Dynamic of Microbial Community Structure and Water Quality of the Marine Bathing Beach

Dalian Jinshitan beach was chosen to evaluate the impact of a typhoon on the bacterial community structure and water quality of a marine bathing beach. The concentration of enterococci was determined by the cultivation method. The bacterial community structure and abundance were analyzed using the 16S rDNA next-generation sequencing and qPCR methods. Results showed that the abundance of cultivable enterococci both in alongshore and offshore seawater increased, while it decreased in dry, wet and submerged sand. The water quality deteriorated immediately after the typhoon, and nearly recovered one month after the typhoon. The typhoon event also decreased the bacterial abundance and changed the bacterial community of the beach. Sphingomonadaceae and Rhodobacteraceae significantly increased in seawater and decreased in dry sand immediately after the typhoon. Human and other fecal taxa increased in water and sand. One month after the typhoon, the diversity and many dominant bacterial taxa nearly recovered in seawater and wet sand. Our work shows that the typhoon changed the bacterial dynamics, deteriorated the water quality and proved the transportation of bacterial taxa and input of fecal pollution between water and beach sand or land. Apart from the impact of the typhoon, the geographical location was another important factor in the changed bacterial community.

Local differences in paleohydrology have stronger influence on plant biomarkers than regional climate change across two Paleogene Laramide Basins, Wyoming, USA

Plant biomarkers in the geologic record are commonly used to reconstruct paleovegetation and paleoclimate. Yet the representativeness of these biomarker-based reconstructions is not entirely straightforward because both local and regional climatic influences are convolved in the recovered biomarker composition. Furthermore, geomorphic processes including sedimentation, deposition, burial rates, and surface and groundwater levels can also influence biomarker preservation and taphonomy. The combination of leaf compression fossils with the biomarker record provides a robust framework in which ecological reconstructions can be made. Herein we present a case study examining the biomarker responses to a major regional climatic signal, the Paleocene Eocene Thermal Maximum (PETM), from local effects in two basins with different paleohydrology: the Hanna Basin (HB) and Bighorn Basin (BHB), in Wyoming (USA). Data collected include (1) leaf wax n-alkanes and plant-derived non-steroidal di- and triterpenoids, (2) pristane/phytane vs. terrestrial to aquatic ratio (Pr/Ph:TAR), plant material source proxy (Paq), hopanes, and bacteria which provide constraints on organic matter sourcing, redox conditions, and bacterial inputs, and (3) carbon (δ13Cn-alk) and hydrogen (δ2Hn-alk) isotopic compositions of plant wax n-alkanes. We find the two basins differed significantly in weight % total organic carbon (TOC), alkane and terpenoid concentrations, and δ13Cn-alk and δ2Hn-alk values. We examined the effects of δ13Catm on δ13Cn-alk values by assuming comparable regional climate in the two basins. Differences in paleohydrology and paleovegetation were assessed using the above proxies and previously published paleobotanical data. Local paleohydrologic conditions, an abiotic factor, likely drove variability in plant biomarker preservation and paleovegetation structure. Notably, an abundance of water in the HB during the PETM created a conifer refugium while drying within the BHB caused a local extirpation. This study highlights the importance of considering the influence of local environment, specifically paleohydrology, on the local fossil record and the need to compare multiple sites to disentangle regional climate effects on terrestrial plant communities. Distinguishing local from regional differences is imperative if we are to understand how ecosystems respond to future climate change.

Analysis of a continuously stirred two tank reactor cascade with Haldane kinetics

Biological reactors are employed in industrial applications to break down organic waste. We view the cascade of two open loop continuously stirred tank reactors with Haldane growth function as chemostats with bacterial inputs. A function of some of the reactor parameters is derived, the sign of which determines the maximum number of critical points a reactor can have. This allows us to determine the parameter combinations which ensure a reactor has only a single critical point for all bacterial removal rates (dilution rate plus death rate). Where a simple condition on the above function is confirmed to hold, if the first reactor in a cascade only has a single critical point for all bacterial removal rates then, the next reactor will also only have a single critical point for all bacterial removal rates. A global stability result is also given for some of these cases. A simple proof is given for the local stability of critical points of a reactor with a general class of bacterial growth functions, bacteria and substrate input, and a death rate. For the special case where the first reactor has zero bacteria input, we compare a two reactor cascade with a single reactor under various conditions, long and short residence times, and different death rates. This follows the pattern of similar papers that considered cascades using the Monod and Contois growth functions.

Characterization of organic matter from the Cretaceous sedimentary and volcano-sedimentary strata from Livingston Island, Antarctic Peninsula: Insights from organic petrology, molecular proxies and carbon and hydrogen isotopes

Rock samples from the Early to Late Cretaceous marine and non-marine strata of Livingston Island, South Shetland Islands, Antarctica, were studied. Organic matter (OM) originates predominantly from terrestrially-derived organic particles. Mostly highly fragmented plant remains, and locally vitrinite/semifusinite as part of plant compressions/imprints or thin vitrain/fusain lenses, are present. Bulk geochemical results indicate Type III kerogen with poor hydrocarbon generation potential. Tmax and molecular proxies (i.e hopane and sterane isomerization ratios, MPI-1, etc.), together with the local presence of oily droplets, argue for an early oil window maturity, probably caused by regional volcanic activity. In contrast to the results of petrography and Rock-Eval pyrolysis, molecular composition of extractable OM is dominated by short-chain n-alkanes, saturated fatty acids (FAs) and n-alkanols implying a major contribution of autochthonous OM. This contradiction is most likely caused by decomposition of OM from plant tissues, the predominance of wood remains (i.e., xylites) and the low input of FAs from cuticular waxes. The presence of terrestrial OM is indicated by plant wax-derived lipids and resinous compounds. Prominent even carbon number predominance in the short-chain n-alkane range argue for deposition/decomposition under reducing environmental settings, most probably caused by high sedimentation rates and quick burial. Terpenoid biomarker compositions indicate contribution from conifers, whereas input from phytoplankton/algal organic matter is evidenced by C27 steroids, the presence of 4-methyl steranes and dinosterol. Low concentrations of hopanoids and C15 and C17 branched n-FAs argue for limited bacterial input. Only in one sample from Hannah Point, a difference between the δ13C values of the short-chain versus long-chain n-alkanes was obtained, arguing for mixed OM sources (i.e., autochthonous input, land plants). The presence of charred organic matter in most of the studied samples, together with the considerable concentrations of combustion-derived PAHs, argue for the common occurrence of wildfires within the terrestrial environments. Despite the active volcanic arc settings, the compound specific carbon and hydrogen isotopes do not record significant changes in climatic/hydrological conditions, although some short-term events cannot be ruled out. Instead, the δ13C and δ2H isotopic fractionation is most likely caused by the specific biosynthetic mechanisms of the plants and the global carbon and hydrogen cycles.

GEOCHEMICAL COMPOSITION OF ORDOVICIAN OILS FROM THE TUOPUTAI REGION, NORTHERN TARIM BASIN, NW CHINA: SOURCE ROCK CORRELATION

Molecular and stable carbon isotope compositions of 46 Ordovician crude oil samples from wells in the Tuoputai region of the northern Tarim Basin were investigated using GC–MS, MRM GC–MS and IRMS to determine their genetic relationships and to identify possible source rocks. Thirty‐three source rock samples from outcrops and cores were also investigated. The oil samples varied from light to heavy crudes and showed very narrow δ13C value ranges for the whole oil, saturated and aromatic fractions. The majority of the oils displayed very similar molecular compositions with relatively high concentrations of n‐alkanes and isoprenoids and low concentrations of terpenoids and steroids. Comparison of the compositions of these crude oils strongly suggested their genetic affinity, while maturity parameters indicated maturity variations from the peak to the late oil generation stages. The samples also showed the characteristics of mixtures of biodegraded and fresh oil charges.Bitumen extracts from Cambrian and Ordovician source rocks were studied in detail. The oil compositions suggested a marine marl source deposited in anoxic, hypersaline conditions with significant bacterial and algal organic matter inputs. The distributions of C26–C28 triaromatic steroids, tricyclic terpanes and regular steranes appear to have been greatly influenced by thermal maturation, making them unreliable for correlating the oils and the source rocks. In contrast, dinosteranes and triaromatic dinosteroids seem not to have been affected by maturation and were more useful for correlation studies. They indicated that there was no or little genetic relationship between the Cambrian – Lower Ordovician source rocks and the oils, but in general suggested a possible Middle – Upper Ordovician source for the oil accumulations in the Tuoputai field. However, the occurrence of triaromatic dinosteroids in oil from well TP28XCX may also suggest a minor contribution from Cambrian – Lower Ordovician source rocks.

Effect of formaldehyde exposure on bacterial communities in simulating indoor environments

Indoor formaldehyde (CH2O) exceeding the recommended level is a severe threat to human health. Few studies have investigated its effect on indoor surface bacterial communities, affecting habitants' health. This study used 20-L glass containers to mimic the indoor environment with bacterial inputs from human oral respiration. The behavior of bacterial communities responding to CH2O varied among the different CH2O levels. The bacterial community structure significantly changed over time in the 0.054 mg·m−3 CH2O group, which varied from the 0.1 mg·m−3 and 0.25 mg·m−3 CH2O groups. The Chao1 and Shannon index significantly increased in the 0.054 mg·m−3 CH2O group at 6 week, while they remained unchanged in the 0.25 mg·m−3 CH2O group. At 12 week, the Chao1 significantly increased in the 0.25 mg·m−3 CH2O group, while it remained unchanged in the 0.054 mg·m−3 CH2O group. Only a few Operational Taxonomic Units (OTUs) significantly correlated with the CH2O concentration. CH2O-induced OTUs mainly belong to the Proteobacteria and Firmicutes. Furthermore, bacterial communities formed at 6 or 12 weeks differed significantly among different CH2O levels. Functional analysis of bacterial communities showed that inferred genes related to chemical degradation and diseases were the highest in the 0.25 mg·m−3 CH2O group at 12 weeks. The development of nematodes fed with bacteria collected at 12 weeks was applied to evaluate the bacterial community's hazards. This showed significantly impaired growth in the 0.1 mg·m−3 and 0.25 mg·m−3 CH2O groups. These findings confirmed that CH2O concentration and exposure time could affect the indoor bacterial community and formed bacterial communities with a possibly more significant hazard to human health after long-term exposure to high CH2O levels.

Normal alkanes in sediments from the Persian Gulf: spatial pattern and implications for autochthonous, allochthonous, and petroleum-originated contaminants.

Surface sediments (n = 124) were gathered to evaluate spatial pattern and source apportionment of ∑24n-alkanes in the Persian Gulf. Moran's I indices revealed that ∑24n-alkanes were randomly distributed in sampling space in general. One noticeable exception was a significant spatial high-high cluster (Z-score = 8.6). Profile of n-alkanes observed for this cluster maximized at n-C20, n-C18, n-C16, and n-C22. Detection of very low carbon preference indices and strong even carbon-numbered predominance suggested that bacterial inputs were the process making this cluster.Based on diagnostic ratios and GIS techniques, in situ production of organic materials was widespread in the studied space. Allochthonous and petroleum-based inputs were not traceable for 73.8% and 24.7% of the area, respectively. Petrogenic source apportionment map proposed that petroleum-originated contaminants were probably limited to 6.32 km2 of the sampling matrix. The biogenic inputs (autochthonous and allochthonous inputs) and not the petroleum contaminants were the main source of n-alkanes deposited.

Biased preservation of Pleistocene climate variability proxies at Olduvai Gorge, Tanzania

Olduvai Gorge in northern Tanzania contains a fossiliferous, well-characterized Pleistocene sedimentary record and provides the opportunity to study the relationships between a changing climate, ecology, and hominin evolution. The Olduvai Gorge Coring Project drilled four cores (1A, 2A, 3A, and 3B) into the depocenter of Paleolake Olduvai in 2014 to achieve increased temporal resolution of local climate and ecological data, and investigate the influence and timing of regional climate and tectonics on local signals. We present high-resolution records of bulk organic carbon isotopes (δ13Corg, ‰) from Cores 2A and 3A, total organic carbon (wt%) from Cores 2A and 3A, and organic carbon‑nitrogen ratios (C:N) from Core 2A. Previous work at Olduvai linked % TOC and δ13Corg to orbitally paced variations in lake depth and ecosystem dynamics from Upper Bed I and Lower Bed II (1.9–1.7 Ma), associated with eccentricity maxima and the presence of a perennial saline-alkaline lake in the basin. Bulk organic geochemical properties in both cores exhibit marked shifts in variance and magnitude at 1.9 and 1.7 Ma. Low % TOC values prior to and following 1.9–1.7 Ma implicate low productivity and/or increased degradation of organic matter, while C:N ratios from Core 2A reflect increased aquatic or bacterial input. Within the 1.9 to 1.7 Ma interval, high % TOC is dominated by terrestrial inputs as evidenced by high C:N ratios, and bulk δ13Corg captures high variability C3–C4 ecosystem dynamics. Climate variability is highest from 1.9–1.7 Ma, but the δ13Corg records are not consistent between Core 2A and Core 3A. From 1.9–1.7 Ma, Core 3A has increased indicators of erosion relative to Core 2A, suggesting a sedimentary aliasing of the δ13Corg record in Core 3A. Outside of the 1.9–1.7 Ma interval, both changes in organic carbon source and preferential preservation of proxies for wet conditions may obscure true climate variability, inviting further investigation into the ability of the Olduvai Gorge sedimentary record to test climate variability hypotheses for hominin evolutionary events.

Comparison of Rosin and Propolis Antimicrobials in Cellulose Acetate Fibers Against Staphylococcus aureus

The quantitative difference in the antibacterial response was measured for pine rosin and propolis against Staphylococcus aureus ATCC 12598. The activity was studied for fibrous networks that form entirely bio-based cellulose-acetate (CA) materials. The analysis considers the effects of bacterial input, additive dosage, solvent type, variation in preparation, as well as the effect of storage time. Based on the results, the electrospun network structure is dependent on the solvent and the concentration of rosin and propolis. Both rosin and propolis improved the cellulose acetate solution processability, yet they formed beads at high concentrations. Rosin and propolis created strong antibacterial properties when these material systems were immersed in the liquid for 24 h at room temperature. The response remained visible for a minimum of two months. The electrospun networks of water and DMAc solvent systems with 1 to 5 wt% rosin content were clearly more efficient (i.e., decrease of 4 to 6 logs in colony forming units per mL) than the propolis networks, even after two months. This efficiency is likely due to the high content of abietic acids present in the rosin, which is based on the Fourier transform infrared spectra. The results of the additional analysis and cell cultivation with dermal fibroblast cells indicated an impairing effect on skin tissue by the rosin at a 1 wt% concentration compared to the pure CA fibers.

Profiling native aquifer bacteria in a uranium roll-front deposit and their role in biogeochemical cycle dynamics: Insights regarding in situ recovery mining

A uranium-mineralized sandy aquifer, planned for mining by means of uranium in situ recovery (U ISR), harbors a reservoir of bacterial life that may influence the biogeochemical cycles surrounding uranium roll-front deposits. Since microorganisms play an important role at all stages of U ISR, a better knowledge of the resident bacteria before any ISR actuations is essential to face environmental quality assessment. The focus here was on the characterization of bacteria residing in an aquifer surrounding a uranium roll-front deposit that forms part of an ISR facility project at Zoovch Ovoo (Mongolia). Water samples were collected following the natural redox zonation inherited in the native aquifer, including the mineralized orebody, as well as compartments located both upstream (oxidized waters) and downstream (reduced waters) of this area. An imposed chemical zonation for all sensitive redox elements through the roll-front system was observed. In addition, high-throughput sequencing data showed that the bacterial community structure was shaped by the redox gradient and oxygen availability. Several interesting bacteria were identified, including sulphate-reducing (e.g. Desulfovibrio, Nitrospira), iron-reducing (e.g. Gallionella, Sideroxydans), iron-oxidizing (e.g. Rhodobacter, Albidiferax, Ferribacterium), and nitrate-reducing bacteria (e.g. Pseudomonas, Aquabacterium), which may also be involved in metal reduction (e.g. Desulfovibrio, Ferribacterium, Pseudomonas, Albidiferax, Caulobacter, Zooglea). Canonical correspondence analysis (CCA) and co-occurrence patterns confirmed strong correlations among the bacterial genera, suggesting either shared/preferred environmental conditions or the performance of similar/complementary functions. As a whole, the bacterial community residing in each aquifer compartment would appear to define an ecologically functional ecosystem, containing suitable microorganisms (e.g. acidophilic bacteria) prone to promote the remediation of the acidified aquifer by natural attenuation. Assessing the composition and structure of the aquifer's native bacteria is a prerequisite for understanding natural attenuation and predicting the role of bacterial input in improving ISR efficiency.