Among the shoulder musculoskeletal disorders (MSDs), rotator cuff disorders are prevalent and known to substantially limit an individual's strength and ability to work. Despite the frequency and cost of rotator cuff injuries, very little research has been done on understanding the load response relationship of rotator cuff muscles. Therefore, this study was aimed at evaluating the strength, endurance and fatigue response of rotator cuff muscles. As a first step, strengths of Supraspinatus, Infraspinatus, and Teres Minor were measured for ten healthy male participants using muscle specific Maximum Voluntary Contractions (MVC). Then, endurance time and surface Electromyography (EMG) data were recorded during 15%, 30%, 45%, and 60% MVC exertions. Infraspinatus exhibited the highest strength followed by Teres Minor and Supraspinatus. Despite the strength differences, small variations were observed in the endurance time between the muscles. The effect of %MVC exertions on endurance time, average muscle activity and muscle fatigue were statistically significant. Median frequency decreased and muscle activation increased with an increase in force exertion levels; however, the changes observed for an increase in the exertion level from 15% to 30% MVC were much higher than the changes observed for an increase in the exertion level from 45% to 60% MVC. Relevance to industryResults of this study indicate that the rotator cuff muscles have different strengths but exhibit very similar endurance and fatigue behavior. These findings can assist ergonomic practitioners with the design and/or evaluation of workplace upper extremity exertions to reduce/manage stress on the rotator cuff muscles and shoulder.