Several lines of evidence suggest corpus callosum (CC) atrophy in mild cognitive impairment (MCI) and Alzheimer's Disease(AD). While MCI patients have anterior CC atrophy, AD patients have both anterior and posterior atrophy, suggesting progressive atrophy of CC in the course of AD. However, the relation between CC volume and cognitive measures in middle aged individuals without AD is not well-examined. Here, we therefore extend our earlier observations on the relation between CC regional volumes and executive functions in middle-aged individuals (50-65years). 61 healthy volunteers (female=31, age=64.09±8.06, years of education=16.90±4.00) participated in the Tata Longitudinal Study of Aging at Bangalore, India. Of these, a subset of 37 individuals were 50-65 years of age (female=21, age=58.56±4.21, years of education=17.02±4.52). All participants underwent neuropsychological testing and Magnetic Resonance Imaging (MRI) scans as per the Alzheimer's Disease Neuroimaging Initiative (ADNI) protocol. CC volumes were acquired for each individual using Freesurfer Software (http://surfer.nmr.mgh.harvard.edu/). Pearson's correlation analysis was conducted to examine the relation between CC volume and executive functions. Executive functions were tested using Controlled Oral Word Association Test (COWAT), digit span, Stroop test and Trail Making Test (TMT). At baseline (entire group), there was a significant positive correlation between COWAT-A score (higher the score, better the performance) and anterior CC-(r=0.561, p=0.001), central CC-(r=0.403,p=0.022) and posterior CC-(r=0.397,p=0.025) volumes. A significant negative correlation was seen between TMT-A Time (greater the time, poorer the performance) and mid-anterior CC-(r=-0.506,p=0.003), central CC-(r=-0.468,p=0.007), posterior CC-(r=-0.458,p=0.008), mid-posterior CC-(r=-0.424,p=0.016) and anterior CC-(r=-0.399,p=0.024) volumes; Stroop-B interference time and mid-posterior CC volume(r=-0.399,p=0.024); and Stroop-C interference time and mid-posterior CC-(r=-0.487,p=0.005), central CC-(r=-0.467,p=0.007), mid-anterior CC-(r=-0.409,p=0.020) and posterior CC-(r=-0.358,p=0.045) volumes. These findings are mirrored in the middle-aged subset, where there was a significant positive correlation between COWAT-A score and anterior CC-(r=0.558,p=0.007) and posterior CC-(r=0.479,p=0.024) volumes; and a significant negative correlation between TMT-A Time and mid-anterior CC-(r=-0.625,p=0.002), central CC-(r=-0.509,p=0.016), posterior CC-(r=-0.497,p=0.019) and anterior CC-(r=-0.469,p=0.028) volumes. Our findings indicate a significant association between CC volume and executive function. Findings suggest that those with lesser CC volume have lower executive function performance. These relationships observable in middle-age (50-65 years), indicate that CC atrophy is a potential early marker of cognitive decline. Longitudinal observations of these individuals will inform whether CC volumes are early markers of cognitive decline.