Asian Elephants Elephas Maximus Research Articles

On the elephant trails: habitat suitability and connectivity for Asian elephants in eastern Indian landscape.

Identifying suitable habitats and conserving corridors are crucial to the long-term conservation of large and conflict-prone animals. Being a flagship species, survival of Asian elephants is threatened by human-induced mortality and habitat modification. We aimed to assess the habitat suitability and connectivity of the Asian elephant Elephas maximus Linnaeus, 1758 habitat in the state of Odisha in eastern India. We followed the ensemble of spatial prediction models using species presence data and five environmental variables. We used least-cost path and circuit theory approaches to identify the spatial connectivity between core habitats for Asian elephants. The results revealed that normalized difference vegetation index (NDVI; variable importance 42%) and terrain ruggedness (19%) are the most influential variables for predicting habitat suitability of species within the study area. Our habitat suitability map estimated 14.6% of Odisha's geographical area (c. 22,442 km2) as highly suitable and 13.3% (c. 20,464 km2) as moderate highly suitable. We identified 58 potential linkages to maintain the habitat connectivity across study area. Furthermore, we identified pinch points, bottlenecks, and high centrality links between core habitats. Our study offers management implications for long-term landscape conservation for Asian elephants in Odisha and highlights priority zones that can help maintain spatial links between elephant habitats.

Mitochondrial genome sequence comparisons indicate that the elephant louse Haematomyzus elephantis (Piaget, 1869) contains cryptic species.

The parvorder Rhynchopthirina contains three currently recognised species of lice that parasitize elephants (both African savanna elephant Loxodonta africana and Asian elephant Elephas maximus), desert warthogs (Phacochoerus aethiopicus) and Red River hogs (Potamochoerus porcus), respectively. The Asian elephant lice and the African savanna elephant lice are currently treated as the same species, Haematomyzus elephantis (Piaget, 1869), based on morphology despite the fact that their hosts diverged 8.4 million years ago. In the current study, we sequenced 23 mitochondrial (mt) genes of African savanna elephant lice collected in South Africa and analysed the sequence divergence between African savanna elephant lice and previously sequenced Asian elephant lice. Sequence comparisons revealed >23% divergence for the 23 mt genes as a whole and ~17% divergence for cox1 gene between African savanna and Asian elephant lice, which were far higher than the divergence expected within a species. Furthermore, the mt gene sequence divergences between these lice are 3.76-4.6 times higher than that between their hosts, the African savanna and Asian elephants, which are expected for the co-divergence and co-evolution between lice and their elephant hosts. We conclude that (1) H. elephantis (Piaget, 1869) contains cryptic species and (2) African savanna and Asian elephant lice are different species genetically that may have co-diverged and co-evolved with their hosts.

Combining Camera Trap Data and Environmental Data to Estimate the Effects of Environmental Gradients on Abundance of the Asian Elephant Elephas maximus in Cambodia

Asian elephant (Elephas maximus) populations in Cambodia are currently declining, and the effect of environmental degradation on the abundance and health of elephants is poorly understood. We used camera trap data from 42 locations between 2016 to 2020 in the southern Cardamom Mountains to investigate the impact of environmental degradation on the abundance and condition of Asian elephants. Camera trap data were organized using CameraSweet software to retrieve both number of individuals and their condition. For a number of individuals, we defined independent captures spatially and temporally. To assess condition, we created a visual scoring system based on past research (Wemmer et al. 2006, Fernando et al. 2009, Morfeld et al. 2014, Wijeyamohan et al. 2014, Morfeld et al. 2016, Schiffmann et al. 2020). This scoring system relies on visual assessment of the muscle and fat in relation to the pelvis, ribs, and back bone. To validate this subjective scoring system, two scorers reviewed elephant captures by using 10 reference photos and then reviewing each other’s assessment in the first five images showing the elephant's body condition. This method minimizes subjective assessment from two scorers. Environmental variables (Suppl. material 1) such as distance to forest edge, forest integrity index, elevation, global human settlements, distance to road, distance to river, night light and forest cover were obtained, then reclassified in ArcGIS to a common 1 km grid. We implemented hierarchical N-mixture models to investigate the impacts of environmental variables on abundance and used cumulative link models to investigate the impact of the same environmental variables on condition. We found that Asian elephant abundance exhibited a significant positive relationship with distance to forest edges, where abundance was greater further away from a forest edge. We found that body condition score exhibited the relationship with forest cover and Forest Landscape Integrity Index, which suggested that grassland and less dense forest support better condition. Moreover, males exhibited significantly higher scores for body condition than females, while babies, juveniles, and subadults all exhibited lower body condition scores compared to adults. The significantly lower body condition of young elephants is concerning and suggests that conservation managers in the region should prioritize environmental conditions that support young elephant health. Our results identify key environmental variables that appear to promote Asian elephant abundance and health in the Cardamom Mountains, thus informing relevant conservation actions to support this endangered species in Cambodia and beyond.

Camera trap surveys reveal a wildlife haven: mammal communities in a tropical forest adjacent to a coal mining landscape in India

Having knowledge of the status and distribution of species in a specific geographic area is crucial for creating efficient conservation strategies. In this study, we evaluated the abundance and diversity of medium to large sized mammals in a tropical forest in India that has been greatly impacted by coal mining. Using camera traps between June 2018 and December 2018, we recorded 27 mammal species over 3432 trap-nights in 81 camera trap stations within the study area. The photo-captured species included both common and high conservation value threatened species, such as tiger Panthera tigris, leopard P. pardus, sloth bear Melursus ursinus, Asian elephant Elephas maximus, Gaur Bos gaurus, Indian pangolin Manis crassicaudata, and four-horned antelope Tetracerus quadricornis. Wild boar Sus scrofa was found to be the most frequently photo-captured and widespread species. Our study provides valuable data on the species inventory and the relative abundance of species in the area, highlighting its significance for mammal conservation. It also emphasizes the need for effective conservation management strategies to protect the remaining forest fragments containing high diversity of mammals, including several threatened species in India.

Anthropogenic driven range expansion of Asian elephant Elephas maximus in an agricultural landscape and its consequences in South West Bengal, India

Alterations of the geographical ranges of animals have become a reason for interactions with humans, leading to various consequences. We describe the pattern of range expansion of Asian elephants (Elephas maximus) and implications for human-elephant interactions in the agricultural landscape of South West Bengal, India. We enquired about past and current sightings of Asian elephants from local people to gather information on range expansion from the 1950 s to 2018. We also collected the records of human deaths and injuries by elephants from 2010 to 2018 from the records of the Forest Department. We employed an occupancy framework to understand the probability of the occurrence of elephants in the landscape from 2010 to 2018. The range of elephants in the landscape increased from ∼ 1200 km2 in the 1950–60 s to ∼ 13200 km2 by 2010–18. The calculated probability of use of grid cells was 36 % and the forest edge had a high influence on the space usage by the elephants. Elephants occurred in an average of 26 grid cells per month, and the number of grid cells with elephants did not differ between the months. A total of 640 attacks on humans occurred, which significantly varied between the years. The probability of elephant occurrence in a grid cell was the major determinant factor for the elephant attacks on humans. Although people respect elephants, there is a sense of intolerance towards them if they enter the crop fields, owing to the small land holdings and the incapacity to endure the monetary losses of the farmers.

Mapping the connectivity–conflict interface to inform conservation

Balancing the competing, and often conflicting, needs of people and wildlife in shared landscapes is a major challenge for conservation science and policy worldwide. Connectivity is critical for wildlife persistence, but dispersing animals may come into conflict with people, leading to severe costs for humans and animals and impeding connectivity. Thus, conflict mitigation and connectivity present an apparent dilemma for conservation. We present a framework to address this dilemma and disentangle the effects of barriers to animal movement and conflict-induced mortality of dispersers on connectivity. We extend random-walk theory to map the connectivity-conflict interface, or areas where frequent animal movement may lead to conflict and conflict in turn impedes connectivity. We illustrate this framework with the endangered Asian elephant Elephas maximus, a species that frequently disperses out of protected areas and comes into conflict with humans. We mapped expected movement across a human-dominated landscape over the short- and long-term, accounting for conflict mortality. Natural and conflict-induced mortality together reduced expected movement and connectivity among populations. Based on model validation, our conflict predictions that explicitly captured animal movement better explained observed conflict than a model that considered distribution alone. Our work highlights the interaction between connectivity and conflict and enables identification of location-specific conflict mitigation strategies that minimize losses to people, while ensuring critical wildlife movement between habitats. By predicting where animal movement and humans collide, we provide a basis to plan for broad-scale conservation and the mutual well-being of wildlife and people in shared landscapes.

Mobile animals and immobile protected areas: improving the coverage of nature reserves for Asian elephant conservation in China

AbstractMany protected areas worldwide have been established to protect the last natural refuges of flagship animal species. However, long-established protected areas do not always match the current distributions of target species under changing environmental conditions. Here we present a case study of the Asian elephant Elephas maximus in Xishuangbanna, south-west China, to evaluate whether the established protected areas match the species’ current distribution and to identify key habitat patches for Asian elephant conservation. Our results show that currently only 24.5% of the predicted Asian elephant distribution in Xishuangbanna is located within Xishuangbanna National Nature Reserve, which was established for elephant conservation. Based on the predicted Asian elephant distribution, we identified the most important habitat patches for elephant conservation in Xishuangbanna. The three most important patches were outside Xishuangbanna National Nature Reserve and together they contained 43.3% of the estimated food resources for Asian elephants in all patches in Xishuangbanna. Thus, we identified a spatial mismatch between immobile protected areas and mobile animals. We recommend the inclusion of the three identified key habitat patches in a new national park currently being planned by the Chinese authorities for the conservation of the Asian elephant.

Using population monitoring programs to detect changes in mammalian communities

The global biodiversity crisis is escalating rapidly, with nearly a million species facing extinction risk in the near future. Monitoring of Essential Biodiversity Variables (EBV; key variables measured to detect changes in biodiversity) over time is important for biodiversity conservation, which in turn necessitates baseline estimates of EBVs. Using camera-trap data for six years (2013–2018) and a Bayesian multispecies occupancy model, we estimated species richness and species-specific occupancy of a mammalian community in Pakke Tiger Reserve, India. We detected 28 species of mammals in our camera traps. The model-based estimate of species richness during the study period was 41 (95 % CRI: 39–42) species, with no evidence of any substantial temporal variation. We found that carnivore and herbivore species richness was higher in greener habitats and that omnivore species richness was higher at lower-elevation sites. Annual average species occupancy probability (at the scale of 4-km2 sampling units) did not vary substantially between years but was lowest in 2014 (0.54, 95 % CRI: 0.20–0.84) and highest in 2013 (0.59, 95 % CRI: 0.27–0.87). Mean species-specific occupancy averaged across the years varied substantially among species, ranging from a low of 0.03 (95 % CRI: 0.00–0.08) for the masked palm civet Paguma larvata to a high of 0.94 (95 % CRI: 0.87–0.98) for the Asian elephant Elephas maximus. Mean occupancy probability was highest for herbivores followed by omnivores and carnivores. Species-specific detection probability (for a 30–60-day survey period) was not influenced by survey effort but increased with body mass for carnivores and decreased with body mass for herbivores and omnivores. We propose a framework for efficient monitoring of species and community-level EBV, utilizing data collected during the monitoring of high-profile species of conservation concern, such as tigers Panthera tigris in India.

Beyond consensus genotyping: a case study on the Asian elephant Elephas maximus

Beyond consensus genotyping: a case study on the Asian elephant Elephas maximus

Bone histology in a fossil elephant (Elephas maximus) from Pulau Bangka, Indonesia

ABSTRACT Analyses of Pleistocene fossil proboscideans have long been used as indirect evidence for climactic and environmental shifts in the Sunda shelf of Southeast Asia. Reconstructing the biological effects of rainforest expansion at the Last Glacial Maximum on elephants can be enhanced by a better understanding of fossil proboscidean palaeobiology. We studied fragmented post-cranial fossil remains of an Asian elephant (Elephas maximus) from Pulau Bangka, an island to the east of Sumatra, hypothesised to be within the Late Pleistocene Sundaland savannah corridor. Bone histology of the humerus, rib, and vertebrae from the Bangka fossil were examined and compared with modern conspecifics to reconstruct remodelling. We measured secondary osteon population density, osteon area, diameter, and infill ratios in Haversian bone. Intra-skeletally, we found that the histology of the largely weight-bearing humerus indicated slower remodelling than that of the ribs and vertebrae, which are less biomechanically constrained. Inter-skeletally, the fossil rib histology showed relatively smaller osteons and Haversian canals when compared to the modern samples. Differences in lifestyles, including range-expansion, may have influenced micro-morphometric differences in elephant rib histology. Our results contribute indirect evidence of the effects of climactic variability in the Sunda palaeoenvironment on Pleistocene fauna.

Serum Insulin-like Growth Factor-1 Is a Biomarker of Testosterone Production and Intact Acrosome in Asian Elephants (Elephas maximus).

Simple SummaryIn Thailand, the low fertility rate of Asian elephants has been identified. Factors contributing to poor semen quality in the elephants are not fully understood. Insulin-like growth factor-1 (IGF-1) is related to male infertility. It plays an essential role in testicular development by stimulating cell proliferation, differentiation, and steroidogenesis. In addition, there is increasing evidence that IGF-1 plays a critical role in spermatogenesis. This may be conducive to finding the causes of poor sperm quality in Asian elephants (Elephas maximus). In the present study, we investigated the relationships among serum IGF-1, serum testosterone level, and semen parameters in seven elephant bulls. The findings suggest that serum IGF-1 concentration is likely to predict sperm quality like acrosome integrity. The further mechanism by which IGF-1 affects sperm quality requires further investigation.The objective of this study was to find relationships among serum IGF-1, serum testosterone, seminal plasma IGF-1 concentrations and semen parameters in Asian elephants (Elephas maximus). A total of 17 ejaculates (one to three ejaculates/bull) were collected from seven captive elephant bulls by performing rectal massage. Before each ejaculation, blood samples were obtained for serum IGF-1 and testosterone assays. Subsequently, the semen characteristics of each ejaculate were evaluated. Mean serum IGF-1 concentration of elephant bulls was estimated as 326.3 ± 114.6 ng/mL (median, 286.2 ng/mL; range, 167.4–542.7 ng/mL). An increase in serum IGF-1 concentration was found to correlate with the percentage of spermatozoa with intact acrosomes. In addition, IGF-1 concentration was positively correlated with testosterone level. However, seminal IGF-1 concentrations could not be detected. In conclusion, our findings suggest that serum IGF-1 concentration is likely a biomarker of normal testicular functions, particularly spermatogenesis in elephants. Moreover, this commercial IGF-1 ELISA is eligible for analyzing serum IGF-1 concentration in Asian elephants.

Drivers of human-megaherbivore interactions in the Eastern and Western Ghats of southern India

The global effort to protect megaherbivore populations is largely dependent on how human-wildlife conflict is identified, prioritized, and remedied. We examined the socio-ecological and landscape-scale factors determining spatial patterns of human-megaherbivore (Asian elephant Elephas maximus and gaur Bos gaurus) interactions across sixteen Forest Divisions in Tamil Nadu, India. Using a systematic grid-based design, we conducted questionnaire-based surveys of 1460 households at the human-wildlife interface adjacent to Protected Areas, Reserve Forest and Fringe Areas. We specifically collected information on elephant and gaur conflict incidents (e.g., human death/injuries, property damage, and crop-raiding), cropland type, extent of crop area and area lost to crop-raiding, from each household. We found that human-elephant conflict increased with percentage of crop cover, diversity of major and minor crops grown, proximity to water source, flat terrain, and lower rates of precipitation. Human-gaur conflict was greatest with a high diversity of major crops, proximity to water source, moderate precipitation, and more undulating terrain. We identified ca. 7900 km2 hotspot area of contiguous high-intensity elephant conflict. For gaur, we identified high-frequency conflict hotspot areas covering ca. 625 km2, which were patchily distributed, highly localised, and attributed mostly to the recent changing land-use patterns. Our findings will help policymakers and park managers in developing landscape-scale human-wildlife conflict mitigation plans in the identified conflict hotspots.

Case report of hook worm Grammocephalus hybridatus and stomach bot Cobboldia elephantis infections in a free-ranging Asian Elephant Elephas maximus in Tamil Nadu, India

Elephants in the wild are susceptible to many gastrointestinal parasites. In the present study, necropsy was conducted on a free-ranging Asian Elephant Elephas maximus female aged about 15 years which died at Coimbatore forest range, Tamil Nadu state, India. The necropsy revealed that the liver was infected with round worms and the stomach was heavily infested with dipteran larvae. These round worms and larvae were collected and processed by dehydrating in ascending grades of alcohol and then cleared in carbolic acid. The cleared samples were mounted and examined under light microscopy for species identification. Faecal samples collected from the rectum were analysed by sedimentation for the presence of helminth eggs. On microscopic examination, the head end of the round worms showed a buccal capsule which possessed a pair of semilunar ventral cutting plates. Male worms showed well-developed bursa at the posterior end. The anterior end of the dipteran larvae showed two powerful oral hooks with cephalopharyngeal skeleton. Anterior spiracle appeared as a short club-shaped tube with 12 lobes. The abdominal segments of the larvae had a row of belt-like triangular spines. The posterior spiracles of the larvae had three longitudinal parallel slits in each spiracle with closed peritreme. Based on the above morphological characters, the round worms and larvae were identified as Grammocephalus hybridatus and Cobboldia elephantis, respectively. Strongyle eggs were identified in the faecal sample based on the morphology of thin shell and segmented yolk. This appears to be the first report of G. hybridatus infection in a free-ranging elephant in Tamil Nadu state, India.

Gastrointestinal parasites in captive wild animals from two Brazilian Zoological Gardens

Keeping animals in zoos is important for the preservation of endangered species. However, captive animals can also be affected by different species of parasites. Herein, we aimed to evaluate the occurrence of gastrointestinal parasites in wild and exotic animals from two zoos in the state of Sergipe, Northeastern Brazil. Fecal samples were obtained by spontaneous defecation of 287 specimens, grouped into mammals (n = 101), birds (n = 99), and reptiles (n = 87). The samples were assessed using two techniques, Mini-FLOTAC and Ziehl-Neelsen, to identify helminths and protozoa, respectively. In total, 60.2% (173/287) of the animals evaluated were positive for some type of gastrointestinal parasite. Among the classes evaluated, mammals (81.1%; 82/101; p-value <0.0001) were mostly affected, followed by birds (56.6%; 56/99) and reptiles (40.2%; 35/87). Furthermore, our findings showed that the parasites Ancylostomatidae and coccidian oocysts were the most abundant among the species. It is important to highlight the first record of some parasites in species in the South America, such as: Ancylostomatidae in Asian Elephant (Elephas maximus) and Brown Bear (Ursus arctos); Toxascaris leonina in Leo (Panthera leo); and Trichostrongyloidea and Ascarididae in Equus quagga burchellii and Lama glama. Taken together, our data showed a high occurrence of gastrointestinal parasites in captive animals, including zoonotic species, which may pose a risk to animal and human public health.

Characteristics of social relationships in a group of captive Asian elephants (Elephas maximus) in the elephant village in Thailand

Wild Asian elephants (Elephas maximus) form complex fission–fusion societies centered on matrilineal groups. In captivity, unrelated females remain in the same group and create social relationships. To better understand well-being in captivity, it is important to investigate the social relationships between females. However, to date, little information is available on this topic. The goal of this study was to clarify the social relationships between captive female Asian elephants using social network analysis. Our subjects were 13 captive Asian elephants at the Elephant Study Center in Surin Province, Thailand. We investigated variations in the frequencies of affiliative behaviors between dyads. Individuals that stayed in the group longer tended to play a more central role in terms of group member connections. We found that two individuals played an important role in strengthening connections, and that their removal influenced group cohesion. Our results revealed that individuals that stayed in the group long-term and that had existing relationships with others in the group tended to build better social connections, regardless of their age. In addition, the existence of some young individuals that show frequent affiliative behaviors may be important for bond strength and therefore, overall group cohesion.

A Review of Human-Elephant Ecological Relations in the Malay Peninsula: Adaptations for Coexistence

Understanding the relationship between humans and elephants is of particular interest for reducing conflict and encouraging coexistence. This paper reviews the ecological relationship between humans and Asian elephants (Elephas maximus) in the rainforests of the Malay Peninsula, examining the extent of differentiation of spatio-temporal and trophic niches. We highlight the strategies that people and elephants use to partition an overlapping fundamental niche. When elephants are present, forest-dwelling people often build above-the-ground shelters; and when people are present, elephants avoid open areas during the day. People are able to access several foods that are out of reach of elephants or inedible; for example, people use water to leach poisons from tubers of wild yams, use blowpipes to kill arboreal game, and climb trees to access honey. We discuss how the transition to agriculture affected the human–elephant relationship by increasing the potential for competition. We conclude that the traditional foraging cultures of the Malay Peninsula are compatible with wildlife conservation.

Assessment of the domestic energy use impacts of unplanned refugee settlements on the forest ecology of Cox’s Bazar, Bangladesh

This study monitors the forest ecology in Himchari National Park, Teknaf Wildlife Sanctuary, and between the areas in Cox's Bazar district, Bangladesh. The area has a rich biodiversity, including globally endangered species such as Asian Elephants (Elephas maximus) and Boilam Trees (Anisoptera scaphula), which are threatened by anthropogenic development, newly refugees’ unplanned settlements and their use of domestic energy. Geographic Information System (GIS) and Landsat satellite images are used to monitor forest coverage for 1995–2018. The Normalized Difference Vegetation Index (NDVI) is applied to quantify forest area. Focus group discussions and questionnaire surveys were conducted to reveal stakeholder perceptions about their dependency on forest resources as ecosystem services. Close to the refugee camp areas, the forest coverage changes to grassland due to the unsustainable forest resource extraction. Despite the free of charge supply of Liquefied Petroleum Gas, the refugees burn 2,380 metric tons of firewood every month to satisfy energy for cooking. Besides, 200,000 households frequently use bamboo, small trees, and shrubs to maintain their dwellings' structure. Thus, deforestation caused by immigration between 2017–2018 is similar to that caused by the effect of climate change, including severe tropical cyclones in 1994–1995. This research identifies domestic energy supply deficiency and impacts, and the need for comparatively durable housing materials to reduce stress on forest resources and health hazards.

Wildlife managers ignore previous knowledge at great risk: the case of Rivaldo, the iconic wild Asian Elephant Elephas maximus L. of the Sigur Region, Nilgiri Biosphere Reserve, India

Management of wildlife depends mostly on scientific data; ignoring this can lead to unintended consequences. We take the case study of the wild male Asian Elephant Rivaldo of the Sigur Region, who was translocated out of his range. Rivaldo returned to his home range within a few days, which could have been expected if scientific publications had been consulted. We suggest that a simple checklist of relevant publications can help park managers to decide on a proper management procedure. We also used a simple Bayesian framework to visually show how the probability of predicting a management outcome is increased by prior knowledge. The expensive and risky effort to relocate the elephant could have been avoided altogether if prior knowledge had been taken into consideration.

Exposure to Toxoplasma gondii in Asian Elephants (Elephas maximus indicus) in Thailand.

Toxoplasma gondii is the causative agent of toxoplasmosis in humans and various animal species worldwide. In Thailand, seroprevalence studies on T. gondii have focused on domestic animals, and information on infections in Asian elephants (Elephas maximus indicus) is scarce. This study was conducted to determine the seroprevalence of T. gondii infection in archival sera collected from 268 elephants living in Thailand. The serum samples were analyzed for anti-T. gondii immunoglobulin G antibodies using the latex agglutination test (LAT) and indirect enzyme-linked immunosorbent assay (iELISA) based on T. gondii lysate antigen (TLA-iELISA) and recombinant T. gondii dense granular antigen 8 protein (TgGRA8-iELISA). The prevalence of antibodies against T. gondii was 45.1% (121/268), 40.7% (109/268), and 44.4% (119/268) using LAT, TLA-iELISA, and TgGRA8-iELISA, respectively. Young elephants had a higher seropositivity rate than elephants aged >40 years (odds ratio = 6.6; p < 0.001; 95% confidence interval: 2.9–15.4). When LAT was used as the reference, TLA-iELISA and TgGRA8-iELISA showed a substantial (κ = 0.69) and moderate (κ = 0.42) agreement, respectively. Although our findings suggest the widespread exposure of Asian elephants to T. gondii in Thailand, the source of infection was not investigated. Therefore, investigation of the predisposing factors associated with toxoplasmosis is necessary to identify the potential risk factors for infection.

Haemato-biochemical changes in tuberculosis infected and healthy Asian elephants (Elephas maximus) from South India

Tuberculosis is known to be a disease of elephants for the past 2000 years. The main causative agent isolated from reported tuberculosis (TB) cases were Mycobacterium tuberculosis. The study focuses on the haematological and serum biochemical changes in the blood of TB infected Asian elephants (Elephas maximus). Twelve apparently healthy elephants and twelve TB infected elephants (confirmed by trunk wash smear positive for acid fast bacilli) were selected for the study. Neonates, pregnant elephants and elephants in musth were not included in the study. The study animals were subjected to haematological and serum biochemical evaluation. The data were analysed statistically. The results showed a significant increase in total leukocyte count, lymphocyte count, monocyte count, thrombocyte count and ESR in TB affected animals compared with apparently healthy animals. Serum creatinine, total bilirubin, direct bilirubin, globulin was significantly high in TB affected animals compared with healthy controls. Assessment of haematological and serum biochemical parameters in TB affected elephants aid in diagnosis and tracking of the infection