Colorimetric sensor array based on a bimetallic PtPd nanozyme for simultaneous discrimination of multiple antioxidants.

Piracetam reverses scopolamine-induced memory disorder in mice: an animal model using behavioral, oxidative, and cholinesterase biomarkers.

HighlightsWe present a pioneering approach to developing a LIG-based wearable electrochemical sensor.LIG was integrated into adhesive bandages, enabling skin-compatibility, flexibility, and easy-to-use sensing.Simultaneous detection of uric acid and ascorbic acid was achieved in artificial sweat.The sensor displayed high sensitivity, reproducibility, and low limits of detection.The scalable sensing platform enabled non-invasive and real-time monitoring of key metabolic biomarkers.

Real-time electrochemical detection of Ascorbic acid in mouse brain homogenate using SCN-wrapped Co@NiO Nanosensor.

Imidacloprid, a neonicotinoid pesticide widely used for controlling agricultural pests, is known to exert toxic effects on non-target aquatic organisms. This study aimed to investigate the toxicological impact of imidacloprid and the potential protective effect of an antioxidant, ascorbic acid, in the freshwater snail Melanopsis praemorsa. Eight experimental groups were established: two controls; three groups exposed to imidacloprid at concentrations of 4.016, 40.16, and 80.32 μg/L; and three groups co-exposed to imidacloprid and ascorbic acid. Test organisms were maintained under defined conditions for 21 days. Following exposure, tissues from the digestive gland, mantle, and foot were dissected and examined at the ultrastructural level using transmission electron microscopy (TEM). Imidacloprid concentrations in the test medium were quantified via LC-MS/MS analysis. Ultrastructural examination revealed pronounced cytopathological alterations in imidacloprid-exposed groups, including cytoplasmic vacuolization, disrupted mitochondrial cristae, degeneration of the rough endoplasmic reticulum and Golgi apparatus, nuclear abnormalities, necrosis, and cellular lysis. In contrast, co-treatment with ascorbic acid led to a noticeable reduction in the severity of these lesions. These findings demonstrate that imidacloprid induces significant tissue damage in Melanopsis praemorsa, while the protective effect of ascorbic acid highlights the potential of this species as a promising bioindicator for monitoring aquatic pesticide contamination.

Seasonal, compositional, and meteorological drivers of PM2.5 oxidative potential: Evidence from a year-long multi-assay study in Melbourne.

Graphitic nanozyme for visual ascorbic acid sensing in commercial beverages.

Bimetallic nanoenzymes Co 3 O 4 /Fe 2 O 3 cooperatively catalyze the generation of ˙OH from H 2 O 2 and are applied for the detection of H 2 O 2 and ascorbic acid.

Enhancing the circular economy of lithium-ion batteries: a data-driven optimisation of leaching efficiency using kernel ridge regression.

Dual targeting of human and bacterial hyaluronidases by skincare bioactives: Mechanistic basis and functional evidence.

A sweat sensor for simultaneous detection of multiple thyroid health biomarkers.

Bioengineered selenium nanoparticles synthesized using Ficus hispida ethyl acetate extract exhibit antibacterial and antioxidant activities.

Water-soluble vitamins including thiamine (vitamin B1), riboflavin (vitamin B2), niacin (vitamin B3), pantothenic acid (vitamin B5), pyridoxine (vitamin B6), biotin (vitamin B7), 5-methyltetrahydrofolic acid (vitamin B9), and ascorbic acid (vitamin C) are essential micronutrients functionally important for human health. Reliable and accurate quantification of the water-soluble vitamin profiles is of vital importance for evaluating their deficiency and related diseases. However, simultaneous determination of these water-soluble vitamins in complex biological fluids such as plasma is a challenge. Herein, an automated liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantifying water-soluble vitamins was developed using a robotic workstation. The optimized sample preparation was conducted through a two-step procedure, protein precipitation using trichloroacetic acid (TCA) and liquid-liquid extraction using methyl tert-butyl ether (MTBE). The entire sample preparation workflow was fully automated with a Hamilton robotic workstation. Eight water-soluble vitamins including B1, B2, B3, B5, B6, B7, B9, and C were simultaneously measured in 8min by LC-MS/MS. The automated LC-MS/MS method was validated for measuring human plasma samples in clinical application. The water-soluble vitamins showed good linearity in their calibration ranges. The limits of detection (LOD) and quantification (LOQ) were 0.01-1.73ng/mL and 0.13-7.30ng/mL, respectively, for B-complex vitamins. For vitamin C, the LOD and LOQ were 36.2ng/mL and 72.3ng/mL, respectively. Satisfactory coefficients of variation (CVs) were obtained, with the intra-batch and inter-batch CVs of 0.9-5.3% and 2.5-14.8%, respectively. Recoveries were considered adequate in the range of 83.2-111.9%, 85.1-118.0%, and 76.5-112.2% for high, medium, and low concentration levels of the eight vitamins, respectively. Matrix effect factors were 80.3-116.0%, 73.2-101.7%, and 76.6-110.5% for high, medium, and low concentration levels, respectively. Robustness of the method in routine clinical practice was further demonstrated by quantifying clinical plasma samples in vitamin screening. The automated LC-MS/MS method for the simultaneous determination of water-soluble vitamins in plasma showed great promise for clinical application.

Although Anacardium humile and Anacardium occidentale fruits are widely consumed in Brazil, quantitative data on their bioactive compound content remain scarce. This study aimed to develop and validate a high-performance liquid chromatography mass spectrometry (HPLC‑MS/MS) method for the targeted quantification of 12 metabolites and to assess their antioxidant potential. Twenty-four mature fruit samples were collected from individual trees and purchased from open-air markets in Goiás and the Federal District over three different weeks (during September and October) and were analyzed using a validated method (r > 0.99; relative standard deviation (RSD) < 12.84%; recovery 88-111%). Ascorbic acid was the predominant compound in both species, although notable interspecific differences were observed for catechin, isoquercetin, and gallic acid. Principal component analysis (PCA) confirmed distinct chemical profiles between species. Antioxidant activity varied significantly, with the effective concentration (EC50) values ranging from 65.67 to 381.94 g fruit per g 2,2-diphenyl-1-picrylhydrazyl (DPPH) for A. occidentale and from 85.37 to 227.62 for A. humile. These findings underscore the importance of robust analytical techniques for characterizing the complex phytochemistry of native Brazilian fruits.

Green emitting nitrogen-doped carbon dots as a dual recognition platform for sensitive and selective detection of 2,4,6-trinitrotoluene and glucose in wastewater.

The preparation of ratio-fluorescent/T1 weight dual mode imaging material PAA/manganese dioxide/graphene quantum dots composites and its application on quantitative analysis of H3PO4.

Green-emissive carbon dots for multitarget staining of cells, fungi, and bacteria: Insights into mechanisms and clinical applications.

Botryosphaeran is an exocellular (1 → 3)(1 → 6)-β-d-glucan that promotes significant metabolic effects in male rats, as antiobesogenic and hypoglycemic effects. This study aimed to investigate its metabolic effects in female Wistar rats subjected to a high-fat high-sucrose diet. Obesity induction resulted in increased body weight, accumulation of adipose tissue, glucose intolerance, insulin resistance, hepatomegaly and high levels of TBARS (oxidative stress marker) in the liver, compared with the controls; all differences were statistically significant (p ˂0.05). Treatment with botryosphaeran (12 mg/kg/day; 15 days) significantly reduced the weight gain (p ˂0.01), the retroperitoneal adipose tissue (-29.7%, p ˂0.05), and corrected glucose intolerance with a 8.32% reduction in the area under the curve (AUC, p ˂0.05), relative to untreated obese rats. Furthermore, botryosphaeran reduced the levels of TBARS (-45.4%, p ˂0.05) in liver, reducing oxidative stress. Additionally, no differences were observed in the liver for protein carbonyls, superoxide dismutase, catalase, glutathione peroxidase, and ascorbic acid. In conclusion, botryosphaeran was observed to promote a significant antiobesogenic effect, promoting an expressive loss in body-weight, reduction of adipose tissue, correction of glucose intolerance and promoting an antioxidant effect in the female rats.

Light-driven hydrogen evolution with carbon quantum dots and an oligoether-bearing [FeFe]‑hydrogenase mimic in aqueous solution.

Simultaneous total antioxidant capacity assessment and ascorbic acid accurate quantification utilizing a H2O2-independent colorimetric-fluorescent sensor array.