Aquatic Herb Research Articles

Reintroduction by transplanting seedlings from natural regeneration: an alternative for ecological restoration?

Collecting and transplanting wild seedlings and mature plants have been used in restoration projects to reduce costs and increase conservation of local genetic material. We review the published literature on restoration projects that used transplanted seedlings and mature plants for ecological restoration. Searching the Web of Science platform using five keywords for articles published in 1945–2023 resulted in 621 articles, with only 51 (9%) of them relevant to the topic. We categorized these 51 publications by ecosystem type, the number and growth forms of species, plant survival rate, as well as the time and cost of transplantation. Forests and wetlands were the most researched ecosystems, and most articles studied tropical forests and the southeastern region of Brazil. Nearly half of the studies (44%) used trees for transplantation, and 73% focused on only one species. In wetlands, mainly aquatic herbs were transplanted (38%). Survival was highest in wetlands (lakes) and lowest in temperate and tropical forests, regardless of the number of species or growth forms. Only three articles (7%) included costs or the time frame of the process of transplantation or monitoring. Despite having few published studies, this practice has high potential for the ecological restoration of tropical and temperate forests. Seedlings and adult plants from natural regeneration can be transplanted to complement or even replace conventional methods of planting nursery seedlings and direct sowing.

Qualitative and GC-MS analysis of medicinally potent aquatic herb Pistia stratiotes L. Assam, India

Pistia stratiotes L. belongs to Araceae family, is a free-floating aquatic plant found in rivers, lakes, and ponds It is commonly known as water cabbage or water lettuce. P. stratiotes stands out as a prominent Ayurvedic remedy, extensively utilized over time for treating a multitude of ailments, The present study was aimed to carry out the detailed preliminary phytochemical and GC-MS analysis of the leaves and root of P. stratiotes. The preliminary phytochemical analysis of P. stratiotes indicated the presence of alkaloids, carbohydrades, reducing sugars, glycosides, flavonoids, phenolic, tannins, triterpinoids, saponins, proteins and amino acids. GC-MS analysis was also carried out to detect the phytoconstituents present in the methanolic, hexane and ethylacetate extract of leaves and root of P. stratiotes.

Observations on the Pollination in <I>Nechamandra alternifolia</I> (Roxb.) Thw.

While studying the ecology of low lying lands in and around Calcutta, some observations were made on the mechanism of pollination in Nechamandra alternifolia, a common aquatic herb of Hydrocharitaceae. The mode of pollination in this plant indicates that the phenomenon is little influenced by the surface depression formed by tepals of the pistillate flowers. It takes place by the direct contact of the anthers of the floating male flowers on a calm surface of water with die stigmatic lobes of the female flower.

A freshwater ecosystem once existed in the Rajasthan desert: evidence from a fossil of the aquatic herb Nelumbo

Nelumbonaceae encompasses aquatic plant lineages that represent major components in the modern tropical wetland ecosystems of India. The group is characterized by its distinctive reproductive receptacle incorporating in situ fruitlets and vegetative organs that are well-represented in the fossil record. Unusually, though, no Nelumbo reproductive fossils have yet been identified from the Cenozoic of India. Here, we report the first such fossil reproductive organ from the aquatic perennial herb Nelumbo, which was recovered in the lower Eocene Palana Formation at the Gurha lignite mine in Rajasthan, western India. The fossil preserves an aggregate fruit with 28 indehiscent, apocarpous and globose to oblong fruitlets embedded within an accrescent receptacle, and a single small central cavity or apical remains of a stigma in each fruitlet. Comparisons with extant and extinct Nelumbo species support attribution to Nelumbo sp. Our unexpected discovery of a fully aquatic plant in the Rajasthan Basin during the early Eocene indicates that a freshwater ecosystem existed there ca 55 ± 10 million years ago; this contrasts starkly with the arid climatic conditions predominant in Rajasthan today. Raman Patel† [ ramanpatel2142@gmail.com ], Department of Geology, Hemvati Nandan Bahuguna Garhwal University, Srinagar, 246174 Uttarakhand, India Ashif Ali† [ aashifli0011@gmail.com ], Palaeobotany, Palynology, and Plant Evolution Laboratory, Department of Botany, Sidho-Kanho-Birsha University, Ranchi Road, Purulia-723104, India Rajendra Singh Rana [ rajendra.rana1@gmail.com ], Department of Geology, Hemvati Nandan Bahuguna Garhwal University, Srinagar, 246174 Uttarakhand, India Mahasin Ali Khan* [ khan.mahasinali@gmail.com ], Palaeobotany, Palynology, and Plant Evolution Laboratory, Department of Botany, Sidho-Kanho-Birsha University, Ranchi Road, Purulia-723104, India.

Confirmed record of Bacopa sessiliflora (Benth.) Edwall (Plantaginaceae) in the Brazilian Cerrado

Bacopa sessiliflora (Benth.) Edwall (Plantaginaceae) is an aquatic herb that inhabits the banks of watercourses in the Neotropics. In this study, we confirmed the occurrence of the species in the Cerrado following a field collection on the banks of the Preto River, in eastern Maranhão state, Brazil, and the study of herbarium materials. A morphological description, illustrations, phenological data, and a distribution map of B. sessiliflora are provided. The new data highlight the importance of permanent protected areas, especially riverbanks, as crucial habitats for increasing our understanding of plant biodiversity in Maranhão.

Potential role of damselflies in the pollination of Callitriche rimosa Fasset

AbstractWhile most flowering plant species rely on animal pollinators, approximately 10% of angiosperms utilize wind or water for pollination. Moreover, certain species can have mixed pollination system—ambophily—wherein both biotic and abiotic agents contribute to pollen transport. Upon frequent observation of damselflies (Coenagrionidae) perching on Callitriche rimosa Fassetv (Plantaginaceae), an aquatic herb with small flowers protruding above the water's surface, we hypothesized their potential contribution to the pollination of this primarily abiotically pollinated plant. Observations were conducted along the shores of a pond in southern Brazil. Damselflies were seen carrying pollen grains on their legs from C. rimosa leaves, where pollen falls from the anthers and remains exposed. Given damselflies' activity levels during summer, incidental contact with C. rimosa reproductive structures facilitates cross‐pollination. This interaction may facilitate both plants and insects, considering damselflies' dependence on freshwater bodies and use of C. rimosa leaves for perching. Accessory pollen presentation on leaves may convert occasional visitors into pollinators, expanding the pollination niche of C. rimosa. Recognizing lesser‐known pollinators like damselflies is crucial for understanding plant reproduction in diverse ecosystems.

A 1 Ma sedimentary ancient DNA (sedaDNA) record of catchment vegetation changes and the developmental history of tropical Lake Towuti (Sulawesi, Indonesia).

Studying past ecosystems from ancient environmental DNA preserved in lake sediments (sedaDNA) is a rapidly expanding field. This research has mainly involved Holocene sediments from lakes in cool climates, with little known about the suitability of sedaDNA to reconstruct substantially older ecosystems in the warm tropics. Here, we report the successful recovery of chloroplast trnL (UAA) sequences (trnL-P6 loop) from the sedimentary record of Lake Towuti (Sulawesi, Indonesia) to elucidate changes in regional tropical vegetation assemblages during the lake's Late Quaternary paleodepositional history. After the stringent removal of contaminants and sequence artifacts, taxonomic assignment of the remaining genuine trnL-P6 reads showed that native nitrogen-fixing legumes, C3 grasses, and shallow wetland vegetation (Alocasia) were most strongly associated with >1-million-year-old (>1 Ma) peats and silts (114-98.8 m composite depth; mcd), which were deposited in a landscape of active river channels, shallow lakes, and peat-swamps. A statistically significant shift toward partly submerged shoreline vegetation that was likely rooted in anoxic muddy soils (i.e., peatland forest trees and wetland C3 grasses (Oryzaceae) and nutrient-demanding aquatic herbs (presumably Oenanthe javanica)) occurred at 76 mcd (~0.8 Ma), ~0.2 Ma after the transition into a permanent lake. This wetland vegetation was most strongly associated with diatom ooze (46-37 mcd), thought to be deposited during maximum nutrient availability and primary productivity. Herbs (Brassicaceae), trees/shrubs (Fabaceae and Theaceae), and C3 grasses correlated with inorganic parameters, indicating increased drainage of ultramafic sediments and laterite soils from the lakes' catchment, particularly at times of inferred drying. Downcore variability in trnL-P6 from tropical forest trees (Toona), shady ground cover herbs (Zingiberaceae), and tree orchids (Luisia) most strongly correlated with sediments of a predominantly felsic signature considered to be originating from the catchment of the Loeha River draining into Lake Towuti during wetter climate conditions. However, the co-correlation with dry climate-adapted trees (i.e., Castanopsis or Lithocarpus) plus C4 grasses suggests that increased precipitation seasonality also contributed to the increased drainage of felsic Loeha River sediments. This multiproxy approach shows that despite elevated insitu temperatures, tropical lake sediments potentially comprise long-term archives of ancient environmental DNA for reconstructing ecosystems, which warrants further exploration.

Phytohormonal regulation determines the organization pattern of shoot aerenchyma in greater duckweed (Spirodela polyrhiza).

Airspace or aerenchyma is crucial for plant development and acclimation to stresses such as hypoxia, drought, and nutritional deficiency. Although ethylene-mediated signaling cascades are known to regulate aerenchyma formation in stems and roots under hypoxic conditions, the precise mechanisms remain unclear. Moreover, the cellular dynamics underlying airspace formation in shoots are poorly understood. We investigated the stage-dependent structural dynamics of shoot aerenchyma in greater duckweed (Spirodela polyrhiza), a fast-growing aquatic herb with well-developed aerenchyma in its floating fronds. Using X-ray micro-computed tomography and histological analysis, we showed that the spatial framework of aerenchyma is established before frond volume increases, driven by cell division and expansion. The substomatal cavity connecting aerenchyma to stomata formed via programmed cell death (PCD) and was closely associated with guard cell development. Additionally, transcriptome analysis and pharmacological studies revealed that the organization of aerenchyma in greater duckweed is determined by the interplay between PCD and proliferation. This balance is governed by spatiotemporal regulation of phytohormone signaling involving ethylene, abscisic acid, and salicylic acid. Overall, our study reveals the structural dynamics and phytohormonal regulation underlying aerenchyma development in duckweed, improving our understanding of how plants establish distinct architectural arrangements. These insights hold the potential for wide-ranging application, not only in comprehending aerenchyma formation across various plant species but also in understanding how airspaces are formed within the leaves of terrestrial plants.

COBERTURA VEGETAL, AÇÃO ANTROPOGÊNICA E PALEOCLIMAS NA CAATINGA

The Caatinga is dominated by a semiarid climate and a high plant biodiversity dealing with anthropogenic disturbance and climate change. Previous studies suggest that Caatinga became a completely soil‐water pulse-dominated forest in response to reduced water availability at the end of the last ice age, around 10 cal kyr BP. This work aimed to identify vegetative and climatic evolution, and the human influence in the Cariri region, one of the driest parts of Brazil. We sampled soil in a temporal pond to identify pollen. The testimony is 170 cm in lenght and achieves 6,216 cal yr BP at the base. Pollen analysis indicates the presence of taxa representative of humid climates, between 6,216 and ca. 5000 cal yr BP, like Azolla, followed by implantation of Caatinga's modern configuration from ca. 4,900 cal yrs BP. After 2,700 cal yr BP, humid episodes occurred, possibly related to the El Niño - La Niña variation across the region, marked by increased algae and aquatic herbs concentrations. The occurrence of human activity species like Caryocar, Dioscorea, Spondias, and Licania suggest possibly landscape manipulation by pre – Columbian populations, and exotic Pinus and Prosopis reveal changes in the tree cover caused by European colonization in the Caatinga. Keywords: Anthropocene; Dry Forest; Paleogeography; Semiarid Region; Sedimentology, Lakes, Lagoons & Swamps.

Folklore use of Common Hydrophytes Found in and around ‘The Jagannath Temple’, Puri, Odisha

Background: Wetlands are rich in aquatic resource which maintains biodiversity and offers support to various aquatic lives. This atmosphere is generally supports hydrophytic plants or hydrophytes.[1] In India wetlands occupy 59.2 million hectres.[2] Puri is a coastal district of Eastern Odisha famous for “The Jagannath Temple” and associated ponds, wells and host of other ancient monuments along with long sea beaches and many rivers. Much survey has been done previously for hydrophytes in India and other districts of Odisha, but not much for Puri district. This work is important as many folklore practicing peoples are using various aquatic herbs for treatment purpose. Methodology: The study was conducted during 2022-2023, involving field visit, meeting with folklore practicing people and taking the photographs of plants for identification. The plants were identified based on The Flora of Odisha[3] and Botany of Bihar & Odisha[4] and contemporary monographs and reviews.[5,6] Results & Discussion: A total number of 36 species belonging to 22 families were documented and their supposed folklore medicinal uses have been recorded.

Temperature is a cryptic factor shaping the geographical pattern of genetic variation in Ceratophyllum demersum across a subtropical freshwater lake

Macrophyte habitats exhibit remarkable heterogeneity, encompassing the spatial variation of abiotic and biotic components such as changes in water conditions and weather as well as anthropogenic stressors. Environmental factors are thought to be important drivers shaping the genetic and epigenetic variation of aquatic plants. However, the links among genetic diversity, epigenetic variation, and environmental variables remain largely unclear, especially for clonal aquatic plants. Here, we performed population genetic and epigenetic analyses in conjunction with habitat discrimination to elucidate the environmental factors driving intraspecies genetic and epigenetic variation in hornwort (Ceratophyllum demersum) in a subtropical lake. Environmental factors were highly correlated with the genetic and epigenetic variation of C. demersum, with temperature being a key driver of the genetic variation. Lower temperature was detected to be correlated with greater genetic and epigenetic variation. Genetic and epigenetic variation were positively driven by water temperature, but were negatively affected by ambient air temperature. These findings indicate that the genetic and epigenetic variation of this clonal aquatic herb is not related to the geographic feature but is instead driven by environmental conditions, and demonstrate the effects of temperature on local genetic and epigenetic variation in aquatic systems.

Phylogenetic relationships of new taxa support repeated pelvic fin loss in mountain catfishes from southern Brazil (Siluriformes: Trichomycteridae)

The presence of paired appendages supported by endoskeleton elements is primarily shared by all gnathostomes, but secondarily lost in different lineages. Absence of pelvic fin and girdle is a condition relatively rare among trichomycterine catfishes, but a recent morphological study suggest that pelvic loss have occurred independently within the genus Cambeva. Herein we perform a molecular phylogenetic analysis encompassing all the five pelvic-less species of Cambeva, including two that are new: Cambeva podostemophila Costa, Feltrin & Katz sp. nov., which is supported by molecular and morphological data as sister to Cambeva poikilos, a species with well-developed pelvic fin and girdle; and Cambeva tourensis Costa, Feltrin & Katz sp. nov., that is sister to Cambeva flavopicta, another pelvic-less species. The ancestral character state reconstruction indicates that pelvic-fin loss occurred four times independently in Cambeva. Field observations indicated that C. podostemophila is closely associated to the podostemacean aquatic herb Podostemum rutifolium, consisting of the first record of trichomycterid–podostemacean association.

Reactive eco-friendly dyeing of natural fabrics using a novel herbal composite containing extracts of Hemigraphis colorata and Bacopa monnieri

Terrestial herbs and aquatic herbs are of natural origin with significant medicinal properties. These properties were selected and aimed to provide antimicrobial and anti-odour activities after finishing with nine different fabric samples in the present study. Hemigraphis colorata and Bacopa monnieri are the two herbs selected and a novel herbal composite (HC1) was developed. Composite was finished onto fabric samples using Pad-dry cure process. Finished fabrics were subjected to antibacterial activity using standard EN ISO 20,645 test method against Escherichia coli and Staphylococcus aureus. To prevent odour from physical activities, aromatic oil was finished onto fabric samples; followed by testing anti-odour activity using Swiss standard SNV 195,651. Test results of antibacterial activity of herbal composite (HC1) finished fabrics revealed good inhibitory action against both test bacteria. Aromatic efficiency of herbal composite finished fabrics showed significant aromatic efficiency. Statistical evaluation determined that there was no significant difference (p–1) between finished and control fabrics in terms of antibacterial activity and aroma. Results for the biocompatibility of the herbal composite finished fabrics revealed that the composite concentrations did not inhibit the growth of fibroblast cells; thus indicating the biocompatibility of the herbal composite finished fabric samples. The present study would be considered highly significant by providing an eco-friendly and sustainable products for developing medical textile products and smart home textile products.

Water lily research: Past, present, and future

The water lily order Nymphaeales includes ~100 species and all grow into aquatic herbs. Most of them are not only economic crops but have, for thousands of years, been regarded as cultural symbols, especially in Buddhism and Hinduism. Water lily order forms an early angiosperm branch, occupying vital roles in evolutionary biology. With the advent and rapid development of omic techniques and bioinformatic software, the research on water lily biology has achieved unprecedented success. In this review, we summarized a series of recent and important findings in water lily research. Genomic analyses of water lilies provide insights into details of their evolutionary history and ecological adaptation. The genomes also provide rich resources for genetic studies and molecular breeding. The opening and closing rhythm of flowering is controlled by auxin and the candidate genes are proposed. Part of the genes responsible for floral scents and floral colors in <italic>Nymphaea colorata</italic> have been studied in-depth for their functions. Metabolomic profiling reveals the anthocyanins responsible for the floral color formation and the volatile organic compounds as floral scent molecules. Phenotypic studies surveyed the diverse traits from flowers to leaves and bulb roots, and some of these studies reveal the link between genes and phenotypes. However, genes responsible for some critical traits such as the growth of water lily plants, such as the stem, bulb formation, and the initiation of vivipary are still unknown. Finally, we propose potential future research of water lilies, including genetics, breeding, and industrialization.

Fossil Fruits of Ceratophyllum from the Upper Eocene and Miocene of South China.

Ceratophyllum L. is a cosmopolitan genus of perennial aquatic herbs that occur in quiet freshwaters. Fossils of this genus have been widely reported from the Northern Hemisphere, most of them occurring in the temperate zone. Here, we describe two species of fossil fruits discovered from subtropical areas of China. The fossil fruit discovered from the upper Eocene Huangniuling Formation of the Maoming Basin is designated as C. cf. muricatum Chamisso, and fruits discovered from the Miocene Erzitang Formation of the Guiping Basin are assigned to the extant species C. demersum L. The discovery of these two fossil species indicates that Ceratophyllum had spread to South China by the late Eocene and their distribution expanded in subtropical China during the Miocene.

Identification of the NAC Transcription Factors and Their Function in ABA and Salinity Response in Nelumbo nucifera.

Nelumbo nucifera Gaertn. is an important perennial aquatic herb that has high ornamental, edible, medicinal, and economic value, being widely distributed and used in China. The NAC superfamily (NAM, ATAF1/2, CUC2) plays critical roles in plant growth, development, and response to abiotic and biotic stresses. Though there have been a few reports about NAC genes in lotus, systematic analysis is still relatively lacking. The present study aimed to characterize all the NAC genes in the lotus and obtain better insights on the NnNACs in response to salt stress by depending on ABA signaling. Here, 97 NAC genes were identified by searching the whole lotus genome based on the raw HMM models of the conserved NAM domain and NAC domain. They were characterized by bioinformatics analysis and divided into 18 subgroups based on the phylogenetic tree. Cis-element analysis demonstrated that NAC genes are responsive to biotic and abiotic stresses, light, low temperature, and plant hormones. Meanwhile, NAC genes had tissue expression specificity. qRT-PCR analysis indicated that NAC genes could be upregulated or downregulated by NaCl treatment, ABA, and fluoridone. In addition, NAC016, NAC025, and NAC070, whose encoding genes were significantly induced by NaCl and ABA, were located in the nucleus. Further analysis showed the three NAC proteins had transcriptional activation capabilities. The co-expression network analysis reflected that NAC proteins may form complexes with other proteins to play a role together. Our study provides a theoretical basis for further research to be conducted on the regulatory mechanisms of salinity resistance in the lotus.

Holocene landscape evolution and its interaction with human activities in the southern piedmont of Taihang Mountain, Central China.

Piedmont zones have been witnessing intensive human activities since ancient times. However, it remains unclear when it comes to the environmental mechanism for early humans exploiting piedmont zones. Here we present a case study about the interactions between early human activities and landscape evolution in the piedmont of Taihang Mountain, an area with prominent ecological and cultural significance in Chinese history. Based on chronological and pollen analyses, we reconstruct the regional landscape evolution in the Fengtougang (FTG) site of southern Taihang Mountain during the Holocene. The results show that the area has been dominated by terrestrial plants since the late Longshan culture (4000 BP), including a large number of Pinus, Artemisia, Spiraea, and Gramineae, a few Cattails, and some other aquatic herbs. During the early history (4000-2000 BP), there is a combination of Pinus, Artemisia, Spiraea, Compositae, and Selaginella Chinensis, with a few aquatic plants. Since the late history (500 BP), the Chinese selaginella, Pinus, Selaginella, and Sedge families dominate, with no aquatic plant pollen found. Combining the detailed geoarchaeological survey, grain size analysis, and magnetic susceptibility analysis, we demonstrate that there should be a landscape of extensive floodplain during the early-middle Holocene (10000-4000 BP). During the late Longshan culture (about 4000 BP), the study area should be dominated by a landscape of sparse forest grassland with interlacing rivers and lakes. With river downcutting and watercourse fixation since the late Longshan culture, the flooded area massively shrinks, providing suitable habitat for human settlement. From then on, human activities begin to move to the study area on a large scale, followed by continuous cultural development and thriving early civilization.

TiO2 nanoparticle synthesis, characterization and application to shoot regeneration of water hyssop (Bacopa monnieri L. Pennel) in vitro

ABSTRACT Water hyssop (Bacopa monnieri L. Pennel) is a medicinal aquatic herb used to treat diseases in South Asia. Various regeneration protocols have been developed or modified in vitro to ensure the availability of biomass and secondary metabolites of Bacopa. We applied hydrothermally treated titanium dioxide (TiO2) nanoparticles (NPs) (TiO2-NPs) at different concentrations. Three explants, distal portion of half leaf (DPHL), proximal portion of half leaf (PPHL) and full leaf (FL), were used to evaluate response to TiO2. Regeneration from the three explants in vitro was similar except for shoot length. Application of TiO2-NPs exerted significant, but variable, effects on all parameters except percentage of shoot formation, which was 100%. Interactive effects of explant and TiO2-NPs exhibited significant, but variable, effects on fresh weight and percentage of callus formation. All explants produced more shoots using TiO2-NPs compared to control treatments. DPHL explants with application of 8 mg/l TiO2 produced more shoots than controls. Similarly, FL explant treated with 2 mg/l TiO2-NPs produced more shoots/explant than controls. All concentrations of TiO2-NPs produced significantly longer shoots compared to controls. Increased elongation of shoots justifies use of TiO2-NPs for propagation of plants in vitro during acclimatization. Use of TiO2-NPs for rapid elongation of shoots ultimately fosters survival of plants.

The complete chloroplast genome sequence of Ranunculus pekinensis (L. Liou) Luferov (Ranunculaceae), a species endemic to China

Ranunculus pekinensis (L. Liou) Luferov 1997, a perennial aquatic herb, is endemic to Beijing, China and has high water quality requirements. Because its habitat is under great threat and its population is declining, it is now listed as a national protected plant in China. To provide genomic resources for future research of this endangered species, the complete chloroplast genome sequence of R. pekinensis was assembled and annotated for the first time. The complete chloroplast genome sequence was 156,139 bp in length, containing a large single copy region (LSC) of 85,430 bp and a small single copy region (SSC) of 19,970 bp, which were separated by a pair of 25,367 bp inverted repeat regions (IRs). The complete chloroplast sequence contained 112 unique genes, including 30 tRNA, 4 rRNA, and 78 protein-coding genes. The overall guanine-cytosine (GC) content of the chloroplast genome was 37.8%, and the GC contents of the LSC, SSC, and IR regions were 36.0%, 31.3%, and 43.5%, respectively. Phylogenetic analysis with the reported chloroplast sequences showed that R. pekinensis was closely related to R. bungei Steud. 1841, both of which belonged to Ranunculus Sect. Batrachium DC. 1817. These data will provide essential resources regarding the evolution and conservation of R. pekinensis.

SMRT and Illumina RNA-Seq Identifies Potential Candidate Genes Related to the Double Flower Phenotype and Unveils SsAP2 as a Key Regulator of the Double-Flower Trait in Sagittaria sagittifolia.

Double flowers are one of the important objectives of ornamental plant breeding. Sagittaria sagittifolia is an aquatic herb in the Alismataceae family that is widely used as an ornamental plant in gardens. However, the reference genome has not been published, and the molecular regulatory mechanism of flower formation remains unclear. In this study, single molecule real-time (SMRT) sequencing technology combined with Illumina RNA-Seq was used to perform a more comprehensive analysis of S. sagittifolia for the first time. We obtained high-quality full-length transcripts, including 53,422 complete open reading frames, and identified 5980 transcription factors that belonged to 67 families, with many MADS-box genes involved in flower formation being obtained. The transcription factors regulated by plant hormone signals played an important role in the development of double flowers. We also identified an AP2 orthologous gene, SsAP2, with a deletion of the binding site for miR172, that overexpressed SsAP2 in S. sagittifolia and exhibited a delayed flowering time and an increased number of petals. This study is the first report of a full-length transcriptome of S. sagittifolia. These reference transcripts will be valuable resources for the analysis of gene structures and sequences, which provide a theoretical basis for the molecular regulatory mechanism governing the formation of double flowers.