Application Of EPTC Research Articles

Application Timing Influences Purple Nutsedge (Cyperus rotundus) and Yellow Nutsedge (Cyperus esculentus) Susceptibility to EPTC and Fomesafen

Purple and yellow nutsedge are problematic weeds in Florida small fruit and vegetable production. EPTC and fomesafen are PRE herbicides that suppress both nutsedge species, but field application in Florida has shown control to be erratic. Greenhouse experiments were conducted in Gainesville, FL, from May to August 2014 and in Wimauma, FL, from March to May 2015 to investigate susceptibility of purple and yellow nutsedge to EPTC and fomesafen applications. Treatments included EPTC at 2.91 kg ai ha−1and fomesafen at 0.42 kg ai ha−1at 0, 3, 6, 9, 12, and 15 d after planting (DAP) tubers, plus a nontreated control. EPTC and fomesafen applications averaged across timings decreased purple and yellow nutsedge emergence, shoot height, leaf number, and shoot mass compared to the nontreated control. Herbicide applications 0 DAP reduced purple nutsedge emergence greater than 65% compared to the nontreated control and caused at least 74% injury 4 wk after planting. Herbicide applications 0 DAP decreased yellow nutsedge emergence and shoot mass compared to the nontreated control by at least 86 and 93%, respectively. Applications of EPTC and fomesafen have the ability to suppress short-term purple and yellow nutsedge growth. Applications made at or prior to tuber sprouting maximize herbicide efficacy.

Tolerance of Otebo Bean (Phaseolus vulgaris) to New Herbicides in Ontario

Weed management is a major production issue facing otebo bean growers in Ontario. Field trials were conducted at six Ontario locations during a 2-yr period (2003 and 2004) to evaluate the tolerance of otebo bean to the preplant incorporated (PPI) application of EPTC at 4,400 and 8,800 g ai/ha, trifluralin at 1,155 and 2,310 g ai/ha, dimethenamid at 1,250 and 2,500 g ai/ha,S-metolachlor at 1,600 and 3,200 g ai/ha, and imazethapyr at 75 and 150 g ai/ha. EPTC, trifluralin, dimethenamid, andS-metolachlor applied PPI resulted in minimal (less than 5%) visual injury and with exception of the low rate of dimethenamid causing a 16% reduction in shoot dry weight and the high rate causing an 8% plant height reduction had no adverse effect on plant height, shoot dry weight, seed moisture content, and yield. Imazethapyr applied PPI caused up to 7% visual injury and reduced plant height, shoot dry weight, and yield 8, 18, and 12% at 75 g/ha and 19, 38, and 27% at 150 g/ ha, respectively. Seed moisture content was also reduced by 0.4% with both rates. Based on these results, otebo bean is not tolerant of imazethapyr applied PPI at rates as low as 75 g/ha, the proposed use rate. EPTC, trifluralin, dimethenamid, andS-metolachlor applied PPI have a 2× rate crop safety margin for use in otebo bean weed management.

Sensitivity of Adzuki Bean (Vigna angularis) to Preplant-Incorporated Herbicides1

There is a limited number of herbicides available for weed control in adzuki bean production in Ontario, Canada. A total of six field trials were conducted in Ontario over a 2-yr period (2003 and 2004) to evaluate tolerance of adzuki bean to preplant incorporated (PPI) application of EPTC (4,400 and 8,800 g ai/ha), trifluralin (1,155 and 2,310 g ai/ha), dimethenamid (1,250 and 2,500 g ai/ha), S-metolachlor (1,600 and 3,200 g ai/ha), and imazethapyr (75 and 150 g ai/ha). All treatments, including the nontreated control, were maintained weed free during the growing season. EPTC and dimethenamid caused as much as 39% visual crop injury and reduced plant height, shoot dry weight, and yield up to 34, 63, and 38%, respectively. Maturity was delayed with the application of EPTC and dimethenamid. Trifluralin caused as much as 9% visual crop injury and decreased plant height up to 11%. There was no effect of trifluralin on shoot dry weight, seed moisture content, and yield. S-metolachlor caused as much as 19% visual crop injury, decreased plant height up to 23%, and reduced shoot dry weight up to 29%. Yield was not affected at the low rate but was decreased 19% at the high rate. There was no effect of S-metolachlor on maturity. Imazethapyr caused up to 6% visual injury but had no adverse effects on plant height, shoot dry weight, seed moisture content, and yield except at the high rate, which caused a 15% reduction in plant height. Based on these results, trifluralin and imazethapyr applied PPI have an adequate margin of crop safety for weed management in adzuki bean production in Ontario.Nomenclature: Dimethenamid; EPTC; imazethapyr; S-metolachlor; trifluralin.Additional index words: Adzuki bean, Vigna angularis.(Willd.) Ohwi and Ohashi ‘Erimo’; crop injury; tolerance.Abbreviations: DAE, days after emergence; PPI, preplant incorporated.

Volatilization of EPTC: Simulation and Measurement

AbstractMany of the organic chemicals used in agricultural production are susceptible to loss from the soil surface to the atmosphere by volatilization. Adequate prediction of the impact of these chemicals on the environment thus requires consideration of both downward movement through the soil to groundwater and upward movement in the gas phase to the atmosphere. We developed a method to mechanistically simulate volatilization within the framework of a conventionally formulated solute transport model and used it to simulate the gas‐phase losses of EPTC, a commonly used volatile herbicide. The model considers efflux of a trace gas at the soil surface to be a process of unsteady diffusion, interrupted intermittently by dispersive events that can be thought of as eddies at the innermost scale. Model results were compared to measurements of volatilization during the first 7 d following application of EPTC, conducted with a Bowen ratio system in a 17‐ha field at Rosemount, MN. The measurements indicated a relatively large initial flux (ca. 150 g ha−1 h−1) that rapidly decreased to negligible levels within a day following application. The model agreed reasonably well on the first day, if a measured value for Henry's constant was used rather than a value estimated from the saturation vapor pressure and the solubility. However, on subsequent days the model considerably overestimated volatilization, regardless of the Henry's constant that was used. It is likely that hysteresis in sorption/desorption, particularly as surface soil dries following herbicide incorporation, may be the primary reason why volatile losses are lower than might be predicted on the basis of equilibrium partitioning theory.

Reducing Johnsongrass (Sorghum halepense) Interference in Corn (Zea mays) with Herbicides and Cultivation

Field studies were conducted in 1990 and 1991 to evaluate johnsongrass control and interference in corn using combinations of nicosulfuron, EPTC + dichlormid, and cultivation. Nicosulfuron applied to 30-cm-tall rhizome johnsongrass provided greater control and corn yield than a later application to 60-cm-tall johnsongrass. Johnsongrass control and corn yield within the later nicosulfuron treatment were improved by a preplant application of EPTC. EPTC and/or cultivation alone provided less johnsongrass control and lower corn yield than nicosulfuron or handweeding.

Effect of Herbicide Rotations on Enhanced Herbicide Biodegradation and Wild-Proso Millet (Panicum miliaceum) Control

A 6-yr field study was conducted to determine the effect of herbicide rotations on enhanced biodegradation of EPTC applied with and without dietholate, and on long-term control of wild-proso millet in sweet corn. Alachlor plus pendimethalin plus cyanazine or cycloate plus cyanazine for 1 yr following previous EPTC plus cyanazine applications reduced enhanced EPTC biodegradation in 3 of 4 and 2 of 3 yr, respectively. Enhanced biodegradation of EPTC applied with dietholate was reduced in 1 of 2 yr when EPTC plus dietholate plus cyanazine treatments were followed for 1 yr by alachlor plus cyanazine, pendimethalin plus cyanazine, or cycloate plus cyanazine, but was not reduced in 2 of 3 yr when EPTC plus dietholate plus cyanazine treatments were followed for 1 yr by alachlor plus pendimethalin plus cyanazine. Dietholate biodegradation was enhanced in soils treated 1, 2, or 3 yr previously with one application of EPTC plus dietholate plus cyanazine. After 3 yr of repeated use, cycloate biodegradation was also enhanced. Continuous use of EPTC plus cyanazine and EPTC plus dietholate plus cyanazine over 6 yr provided an average of only 46 and 64% wild-proso millet control, respectively. When applied once every second, third, or fourth year in rotation with alachlor plus pendimethalin plus cyanazine or cycloate plus cyanazine, EPTC plus cyanazine provided 83, 86, and 95% wild-proso millet control, respectively. EPTC plus dietholate plus cyanazine applied every second, third, or fourth year in rotation with cycloate plus cyanazine, alachlor plus cyanazine, pendimethalin plus cyanazine, or alachlor plus by pendimethalin plus cyanazine provided 88, 91, and 95% wild-proso millet control, respectively.

Biodegradation of Butylate, EPTC, and Extenders in Previously Treated Soils

Laboratory studies were conducted to determine the ability of the extenders dietholate and SC-0058 to prevent enhanced biodegradation of EPTC and butylate applied to Wisconsin soils with different histories of carbamothioate herbicide use. Enhanced EPTC and butylate biodegradation occurred in soils previously treated with those herbicides. Enhanced biodegradation of dietholate occurred on soils previously treated with that extender plus either EPTC or butylate. Enhanced dietholate biodegradation was observed when applied alone or in combination with butylate or EPTC. Application with dietholate prevented enhanced biodegradation of butylate but not EPTC even though enhanced biodegradation of dietholate was occurring. Enhanced biodegradation of SC-0058 did not occur. SC-0058 prevented enhanced EPTC and butylate biodegradation even in soils previously treated for three consecutive years with the respective herbicide plus SC-0058. Application of either 1.1 or 2.2 kg/ha SC-0058 plus 6.7 kg/ha EPTC provided equal or better wild proso millet control and sweet corn yields than applications of EPTC alone or EPTC plus dietholate.

Accelerated degradation of thiocarbamate herbicides in Israeli soils following repeated use of vernolate

AbstractAccelerated degradation of vernolate, EPTC and butylate but not of cycloate was detected in soils from three locations in Israel which were treated annually with vernolate. Repeated application of EPTC to soils with and without a history of vernolate application, under laboratory conditions, resulted in a progressive increase in its rate of dissipation with each application. Accelerated degradation of EPTC was also rapidly induced by mixing small amounts (5%) of soil with a history of vernolate treatment with soil that had never received vernolate. Liberation of 14CO2 from [14C]EPTC was more rapid in vernolate‐treated soils than in untreated soils, indicating a development of microbial populations in soil capable of rapidly degrading the EPTC. Degradation of [14C]EPTC was faster in soil previously cropped with maize than in non‐cropped soil, but slower in soils cropped with cotton or peanuts.

Duration of Enhanced Soil Degradation of EPTC as Influenced by Herbicide Rotation, Time, and Location

Soils did not remain enhanced for EPTC degradation when EPTC was applied in a biennial rotation with cyanazine, cycloate, or alachlor as indicated by laboratory analysis and early-season weed control in the year of EPTC use. Control of late-season weeds was less when EPTC was rotated with alachlor than with a first-time use of EPTC. EPTC degradation remained enhanced in an EPTC-butylate rotation. The reversion rate of soil from an enhanced condition to normality was gradual and varied with location, year, and number of prior EPTC applications. At Clay Center and Scottsbluff, soils reverted to a nonenhanced EPTC degradation rate 18 months after initial EPTC application. At Scottsbluff, soil was not enhanced for EPTC degradation 18 months after the second of two annual EPTC applications. Soil from Clay Center was partially enhanced for EPTC degradation 18 months after the second of two annual EPTC applications but was not enhanced after 30 months.

Populations of EPTC-Degrading Microorganisms in Soils by Accelerated Rates of EPTC Degradation

Reduced effectiveness of carbamothioate (thiocarbamate) herbicides in certain soils has been attributed to rapid herbicide degradation by soil microorganisms. Studies were conducted to determine if greater populations of EPTC (S-ethyl dipropyl carbamothioate)-degrading microorganisms were responsible for increased rates of degradation observed following repeated applications of EPTC to a Grenada silt loam soil. EPTC-degrading microorganism populations, measured with a14C-MPN (most-probable-number) technique, were not larger in soils with accelerated rates of EPTC degradation, and degrader populations did not increase after application of 6 mg EPTC/kg of soil. Degrader populations increased after application of 60 mg EPTC/kg of soil only in soil previously treated for 6 yr with EPTC. Increased rates of metabolism of EPTC were apparently responsible for the increased rates of degradation, rather than increased populations of degraders.

Herbicide Dissipation from Soils with Different Herbicide use Histories

Herbicide dissipation was monitored in soils differing in herbicide use histories. Repeated annual applications over 5 yr enhanced biodegradation of butylate [S-ethyl bis(2-methylpropyl)carbamothioate] and EPTC (S-ethyl dipropylcarbamothioate) but not alachlor {2-chloro-N-(2,6-diethylphenyl)-N-(methoxymethyl)acetamide}, atrazine [6-chloro-N-ethyl-N′-(1-methylethyl)-1,3,5-triazine-2,4-diamine], cyanazine {2-[[(4-chloro-6-(ethylamino)-1,3,5-triazin-2-yl]amino]-2-methylpropanenitrile}, or metolachlor [2-chloro-N-(2-ethyl-6-methylphenyl)-N-(2-methoxy-1-methylethyl)acetamide]. Prior application of butylate or EPTC enhanced biodegradation of the other thiocarbamate herbicide but not alachlor, atrazine, cyanazine, or metolachlor. Prior application of alachlor, atrazine, cyanazine, metolachlor, or trifluralin [2,6-dinitro-N,N-dipropyl-4-(trifluoromethyl)benzenamine] did not enhance biodegradation of butylate or EPTC. Dissipation of EPTC applied with dietholate (O,O-diethyl-O-phenolphosphorothioate) was not enhanced by prior application of alachlor, atrazine, or trifluralin. Prior use of EPTC, EPTC + dietholate, butylate, or cycloate, respectively, enhanced biodegradation of EPTC in 100, 100, 71, and 50% of the experiments, of EPTC applied with dietholate in 57, 100, 60, and 33% of the experiments, of butylate in 33, 40, 100, and 20% of the experiments, and of cycloate in 0, 0, 17, and 0% of the experiments. Prior thiocarbamate herbicide applications usually did not enhance cycloate biodegradation, but soils from two locations without prior pesticide use histories rapidly degraded the herbicide. Storage of soil samples at 25 C for 6 or 12 months before application of EPTC and EPTC + dietholate resulted in less herbicide degradation than storage at 15 C. Differences in prior environmental conditions such as temperature may explain why development of enhanced biodegradation varied between years even in the same field plots.

Applying Eptc and Seeding Alfalfa (Medicago sativa) Simultaneously

Alfalfa (Medicago sativaL. ‘Vernal′) was seeded broadcast or in drill rows spaced 18 cm apart in the same operation that EPTC (S-ethyl dipropylcarbamothioate) was applied. For broadcast seeding, EPTC was applied as herbicide-treated crop seed, as granules mixed with crop seed, or as granules applied with the crop seed, but with the seed and granules carried in separate boxes on the seeder. For drill-row seeding, EPTC was applied between the rows in subsurface lines as a liquid or granular formulation; and within the rows as herbicide-treated seed, as granules mixed with the seed, or as granules carried in a box separate from the seed. Weed control and crop tolerance with the more effective simultaneous applications of EPTC were similar to those with conventional preplant-incorporated applications. To seed alfalfa (either broadcast or in drill rows) and apply EPTC in one operation, the methods wherein untreated seed and a granular formulation of EPTC were applied together but from separate boxes on the seeder were the most practical, because the mechanical components, the seed, and the formulated herbicide are all commercially available.

Physiological interactions between the herbicide EPTC and selected analogues of the antidote naphthalic anhydride on two hybrids of maize

AbstractThe efficacies of nine structural analogues of the herbicide antidote naphthalene‐1,8‐dicarboxylic acid anhydride (naphthalic anhydride, NA) for the protection of maize (Zea mays L. cv. DeKalb XL72AA and DeKalb XL67) against injury by the herbicide S‐ethyl dipropyl(thiocarbamate) (EPTC) were elevated under greenhouse conditions. The chemical analogues of NA tested were: acenaphthenequinone (ACQ); 4‐aminonaphthalene‐1,8‐dicarboxylic acid anhydride (NH2NA); 1,8:4,5‐naphthalenetetracarboxylic acid dianhydride (NDiA); naphthalene‐ 1,8‐carboximide (NHNA); 4‐chloronaphthalene‐1,8‐dicarboxylic acid anhydride (C1NA); biphenyl‐2,2′‐dicarboxylic acid anhydride (diphenic anhydride; DA); 2‐phenylglutaric anhydride (PGA); phthalic anhydride (PHA); phenalen‐1‐one (PA). Pre‐plant incorporated applications of EPTC at 2.2, 4.5, 6.7, and 9.0 kg ha−1 were highly toxic to XL67 maize. Appreciable injury to XL72AA maize by EPTC was observed only with the high rates of EPTC (6.7 and 9.0 kg ha−1). Of the analogues tested PGA and PA were very toxic and inhibited germination of both maize hybrids. NA, ACQ, NH2NA, NDiA, NHNA, C1NA, DA, and PHA applied as seed dressings at 5.0 and 10 g per kg of seed offered satisfactory protection to XL72AA maize against EPTC rates higher than 6.7 kg ha−1. The same antidotes significantly antagonised the EPTC activity against XL67 maize but the overall protection obtained was partial and not agronomically important. The presence of the dicarboxylic anhydride group and of at least one aromatic ring attached directly to the anhydride appeared to be essential for the exhibition of protective activity by the structural analogues of NA. NA was slightly toxic to both hybrids of maize and chlorination of NA increased the phytotoxicity of this molecule. A genetic component that is present in the thiocarbamate‐tolerant XL72AA hybrid but absent from the thiocarbamate‐susceptible XL67 hybrid of maize appeared to be important for the phytotoxic activity of EPTC and may be involved in the protective activity of NA and its structural analogues.

Decomposition of the herbicide EPTC in soils with a history of previous EPTC applications

Abstract The degradation of the herbicide EPTC (S-ethyl N, N-dipropylthiocarbamate) in 3 soils, with and without, previous field exposure to this herbicide was investigated. The influence of storage of soil (12 months) on the subsequent breakdown of EPTC was investigated. Soils with added EPTC were incubated (22°C), and the fate of EPTC was followed using gas liquid chromatogaphy. The herbicide degraded more rapidly in soils with 3 and 4 years of previous' exposure to annual applications of EPTC (6kg/ha) than in those soils without any previous exposure. This more rapid decomposition was evident after storage of soils for I year at ambient temperatures. Microorganisms were responsible for EPTC degradation in these soils. Some of the implications of this phenomenon on soil ecology are discussed.

EPTC ( s-ethyl N, N-dipropylthiocarbamate)-degrading microorganisms isolated from a soil previously exposed to EPTC

EPTC ( s-ethyl N, N-dipropylthiocarbamate)-degrading microorganisms isolated from a soil previously exposed to EPTC

FALL AND SPRING APPLICATIONS OF EPTC AND TWO ANTIDOTES FOR WEED CONTROL IN CORN

EPTC and the antidotes, R25788 (N, N-diallyldichloroacetamide) and R29148 (2, 2, 5-trimethyl-3-dichloroacetyl oxazolidine) were applied to field plots of corn (Zea mays L.) in the fall or spring from the fall of 1975 to the spring of 1978. Soil samples were taken from selected treatments in the fall of 1977 and spring of 1978. Residues of EPTC, R25788, and R29148 were measured by gas liquid chromatography. Much better weed control and higher corn yields were obtained with spring applications than with fall applications. EPTC at the highest rate of 13.4 kg/ha, without the antidotes, did not injure corn. Over 95% of the EPTC present in the soil on 24 Oct. 1977 had disappeared by 30 May 1978. Levels of EPTC on 30 May 1978 were appreciably higher following spring application. Neither R25788 nor R29148 disappeared faster than EPTC from the surface layer of soil.Key words: EPTC, R25788, R29148, soil residues, corn

The Influence of Temperature, Moisture, and Prior EPTC Application on the Degradation of EPTC in Soils

Laboratory and field studies were conducted to compare the degradation rate of EPTC (S-ethyl dipropylthiocarbamate) in soils with a previous history of EPTC application vs. soils with no prior EPTC application. Laboratory experiments showed a rapid breakdown of14C-carbonyl EPTC to14CO2in Kennebec silt loam (sil) and Tripp very fine sandy loam (vfsl) with prior exposure to EPTC. A single prior application of EPTC to Tripp vfsl was sufficient to increase the rate of14CO2evolution. The rate of EPTC degradation in Tripp vfsl with a history of EPTC exposure was dependent on soil moisture from below 3% and independent of moisture above 3%. Patterns of degradation at 5, 15, and 25 C in the Kennebec sil with and without prior history were described by exponential decay. EPTC was degraded more rapidly at 15 and 25 C in the Kennebec sil with a prior EPTC history. Under field conditions the breakdown of EPTC in a Kennebec sil with and without prior exposure to EPTC was similar to laboratory results.

Responses of Several Corn (Zea mays) Cultivars and Weed Species to EPTC With and Without the Antidote R-25788

Field experiments were conducted to determine the effectiveness of the herbicide antidote R-25788 (N,N-diallyl-2,2-dichloroacetamide) in protecting corn (Zea maysL.) cultivars from EPTC (S-ethyl dipropylthiocarbamate) injury. Preplant-incorporated applications of EPTC, with and without R-25788, were made to a fine sandy loam in 1976 and a loamy sand soil in 1977. EPTC at 6.7 kg/ha was 20 to 30% more injurious to the susceptible cultivars grown in sandy, lower organic-matter soil than in fine sandy loam. R-25788 did not decrease the weed-control efficacy of EPTC. The cultivar ‘TXS114’ was highly tolerant to EPTC at 6.7 kg/ha with or without antidote protection. Most of the other EPTC-susceptible cultivars showed significant stand improvement when R-25788 was used in combination with EPTC. R-25788 did not alleviate EPTC phytotoxicity toward ‘XL55’ and ‘XL379’ cultivars. EPTC applied in combination with R-25788 showed no, or only, slight injury to all other cultivars.

Pesticide Effects on the Plant Cuticle: IV. The Effect of EPTC on the Permeability of Cabbage, Bean, and Sugar Beet Cuticle1

Abstract S-ethyl dipropylthiocarbamate (EPTC, 2.24 kg×ha) inhibited epicuticular wax production on developing leaves of cabbage (Brassica oleracea L. Capitata group cv. Market Prize), resulting in an increase in cuticular permeability. This increased penetration of 14C-I-naphthaleneacetic acid (14C-NAA) and increased cuticular transpiration. EPTC-enhanced penetration was a consequence of increased diffusion across the cuticle, and not of active uptake. Application of EPTC increased penetration of NAA 200% in bean (Phaseolus vulgaris L.)and 121% in sugar beet (Beta vulgaris L.). For cabbage, the percent increase in penetration due to EPTC inhibition of cuticle development 7 days after treatment (141 %) was similar to that at 42 days (112%). The effect of EPTC declined until full leaf expansion (28 days after application). Silver nitrate was preferentially taken up by the cuticular ledges of guard cells and the anticlinal walls of epidermal cells, and was greater in leaves from EPTC-treated plants than in those from non-treated plants.

American Germander (Teucrium canadense) Control in Corn (Zea mays)

Experiments were conducted to develop systems for control of American germander [Teucrium canadenseL. var.virginicum(L.) Eat.] in corn (Zea maysL. ‘Trojan TXS 105A’). Soil-incorporated, preemergence, and postemergence herbicide treatments were evaluated in greenhouse studies. Soil incorporated applications of EPTC (S-ethyl dipropylthiocarbamate) + R-25788 (N,N-diallyl-2,2-dichloroacetamide), atrazine [2-chloro-4-(ethylamino)-6-(isopropylamino)-s-triazine] and alachlor [2-chloro-2′,6′-diethyl-N-(methoxymethyl)acetanilide] effectively reduced its growth. Preemergence applications of alachlor and atrazine did not control American germander. Postemergence applications of the dimethylamine salt of 2,4-D [(2,4-dichlorophenoxy)acetic acid] the butoxyethanol ester of 2,4-D and dicamba (3,6-dichoro-o-anisic acid) gave 20 to 41% control. Atrazine + crop oil gave 76 to 84% control. Soil-incorporated treatments of atrazine or EPTC + R-25788 controlled American germander better than alchlor when the rhizomes were planted at depths of 2.5 and 10 cm in greenhouse studies. In field trials American germander was a severe competitor in corn, especially when soil moisture was limited, and had the capability of substantially reducing corn yields when not controlled. EPTC + R-25788 and atrazine applied alone or in combinations as preplant incorporated treatments increased yield significantly and controlled 85 to 99% of the American germander 72 weeks after planting. Atrazine + oil was the only effective postemergence treatment.