Antiulcer Activity Of Ethanolic Extract Research Articles

Antiulcer activity of ethanolic extract of Arctocarpus hirsutus Lam. leaves in albino Wistar rats

Peptic ulcers, a prevalent gastrointestinal disorder, remain a significant health concern. Artocarpus hirsutus Lam., a plant deeply rooted in traditional medicine, has been historically employed to address various health issues. Each component of this plant showcases diverse therapeutic activities, offering a holistic approach to health concerns. This study aimed to explore the gastroprotective and antiulcerogenic potential of the ethanol-based extract derived from Artocarpus hirsutus Lam leaves. Administered orally in 100, 200, and 400mg/kg doses, the extract's effects were compared with established medications - omeprazole (30mg/kg) and sucralfate (100mg/kg). Parameters such as gastric acid volume, gastric pH, ulcer index, total acidity, and free acidity were evaluated. The ethanol-based extract derived from Artocarpus hirsutus Lam. leaves demonstrated activity at doses of 200mg/kg and 400mg/kg, resulting in a marked decrease in free acidity, total acidity, gastric volume, ulcer index, and an increase in gastric pH compared to the ulcer control group. Notably, the ethanol-based extract of leaves of Artocarpus hirsutus Lam. exhibited significant gastroprotective and ant-ulcerogenic effects in both ethanol induced ulcer and pylorus ligated ulcer models. These findings underscore the potential therapeutic value of Artocarpus hirsutus Lam. as an effective agent against peptic ulcers, supporting its traditional use in holistic health practices.

Evaluation of mechanism(s) of action underlying the antioxidant and antiulcer activity of Sesamum indicum leaves extract in experimental rats.

The current study aimed to estimate phytochemical screening, in vitro antioxidant activity, and gastroprotective activity of Sesamum indicum Linn ethanolic extract. The current study was held out by ulceration induced by pylorus ligation and indomethacin-induced ulcer screening models in Wister albino rats. The screening of antiulcer activity of ethanolic extract of S. indicum leaves (EESIL) at the different amounts (100, 200, and 400 mg/kg; per orally for 7 days) was compared with omeprazole as a usual antiulcer drug. Additional parameters such as gastric content, pH, total acidity, pepsin activity ulcer score, free acidity, ulcer index (UI), % inhibition of ulcers, mean mucin, pepsin content, and total protein content were observed. In the pylorus ligation model, the pepsin activity free acidity, pepsin content, UI, total acidity, ulcer score, total protein content, and percentage ulcer inhibition were considerably decreased (P < 0.05 and P < 0.01), and mean mucin and gastric content pH extensively elevated (P < 0.05 and P < 0.01) in EESIL tested groups in the comparison of the control group. Doses (100, 200, and 400 mg/kg p.o.) of EESIL showed dose-reliant gastro protective outcomes, a considerable (P < 0.05 and P < 0.01) decrease in gastric parameters as UI and ulcer score and induction in gastric pH and percentage inhibition of ulcer compared with the control group. Antioxidant, anti-Ulcer, EESIL, and EESIL show antioxidant activity at different concentration. The fallout of the study indicated that the EESIL had improved antiulcer potential due to the decrease in offensive factors and increase in defensive factors.

Investigation on Anti-Ulcer Activity of Momordica dioica Fruits in Wistar Rat

To present study was carried out to investigate antiulcer activity of Ethanolic extract of Momordica dioica fruits in pylorus ligatated and Cold stress induced ulceration in the wistar rats. Pylorus ligation induced ulcer is one of the most widely used methods for studying the effect of drug on gastric secretion. Effect of distilled water in pylorus ligation has caused the accumulation of gastric sectretion and decreased the pH. Ranitidine and Ethanolic extracts of Momordica dioica significantly decreased the gastric volume, total acidity, free acidity, ulcer score, number of ulcer and ulcer index and raise the pH (shown in graph. 1, 2, 3, 4, 5, 6,).Similar studies support our results. Ethanolic extract of Momordica dioica at a dose of (400mg/kg. p.o) showed significant inhibition of ulcerative lesion by 46.33% and 54.66%, respectively, as compared to the control value The Ethanol extract of Momordica dioica fruits possess significant antiulcer properties in a dose dependent manner.

Anti-ulcer Activity of Ethanolic Extract of Alstonia scholaris Bark: An in vivo and in silico Approach

Aims: The present research is focused on screening in vivo anti-ulcer activity using pylorus ligation and ethanol induced ulcer model. &#x0D; Study Design: Fourty eight rats, randomly divided into eight groups, were used in this study. Bark of Alstonia scholaris were air-dried, ground into fine powder and used in the preparation of an ethanolic extract.&#x0D; Place and Duration of Study: Department of Pharmacology, Osmania University, Hyderabad between December 2020 and September 2021.&#x0D; Methodology: Ethanol related ulcer was induced using 1 mL/kg b.w, p.o. Treated rats received ethanolic extract of Alstonia scholaris at various doses of 200 and 400 mg/kg b.w. Ulcer index, % ulcer protection were calculated and histological studies were conducted at 6 hr after pylorous ligation respectively.&#x0D; Results: Pharmacological estimations were done by means of 200 mg/kg, b.w. and 400 mg/kg, b.w. The total acidity and free acidity were decreased, pH was increased and ulcer index was decreased by Ethanolic extract of Alstonia scholaris (EEAS 200 and 400 mg/kg b.w., p.o.) in pylorus ligation model. Treatment with EEAS (200 and 400 mg/kg b.w. p.o.) has significantly decreased the ulcer index by ethanol induced ulcer model. To appreciate the ligand-binding attraction of the dynamic ingredients of the excerpt, docking trainings were accomplished for natural compounds against protein data bank (PDB) ID: 5A5N, PDB ID: 6Q2T, PDB ID: 7MBX, PDB ID: 2FV5. The results revealed that vanillic acid, venoterpine, loganetin, dibutyl phthalate, guaia-3,9- diene, 3,6-Bis[2-methylphenyl]-2,5-dihydropyrrolo[3,4-c] pyrrole-1,4- dione, 2H-1-Benzopyran- 2-one,7-acetyl-8-[acetyloxy]-4, n-hexadecanoic acid, stigmasterol, diospyrolide, D- Friedoolean-14-en-3-one, betulin, lupeol acetate, pentanoic acid and standard drug omeprazole had shown highest glide scores with all the selected proteins which indicate a stronger receptor-ligand binding affinity.&#x0D; Conclusion: From in vivo and in silico results it is evident that ethanolic bark extract of Alstonia scholaris possessed significant anti-ulcer activity.

Evaluation of Antiulcer Activity of Ethanol Extract of Leaves of Lactuca sativa

In this modern era, gastrointestinal disorders are the universal problem. Peptic ulcer is one of the major diseases affecting the human population. It develops due to the imbalance between aggressive factors like acid, pepsin, H. pylori and bile salts and defensive factors like mucous, bicarbonate, blood flow, epithelial cell restoration and prostaglandins. The anti-ulcer activity of Ethanol extract of leaves of Lactuca sativa (EELS) was estimated using the experimental models of acute gastric lesions induced by ethanol, pylorus ligation and cold restraint stress in Wistar albino rats. Animals pre-treated with doses of 250 mg/kg, 500 mg/kg of EELS were statistically analyzed and compared to the standard and control group with the parameters like volume of gastric secretion, total acidity and ulcer index. The results suggested that EELS significantly decreased volume of gastric acid secretion, total acidity and ulcer index in comparison with standard drug Omeprazole. EELS shown significant reduction in lesion index, total affected area and percentage of lesion in comparison with control group in Ethanol induced ulcer in experimental models. The gastric mucosal protective effect of EELS is brought by inhibiting the gastric secretion, which shows it may act like a proton pump inhibitor. Thus the present study indicates that EELS has anti-ulcerogenic potency in Ethanol induced, pylorus ligation and cold restraint stress induced ulcers in rats.&#x0D; Keywords: Antiulcer, ulcer index, cold stress, peptic ulcer, pylorus ligation

Assessment of Antiulcer Activity of Ethanol Extract of Ficus religiosa Fruit in Pylorus Ligated and Aspirin induced Ulceration in Experimental Animals

Ficus religiosa is a medicinal plant and scientifically documented for the pharmacological activities like antidiabetic, anti-inflammatory, antipyretic, etc. The present study was planned to investigate the antiulcer activity of ethanol extract of Ficus religiosa fruit in pylorus ligated and aspirin induced ulceration in experimental animals. The ethanol extract of fruits of Ficus religiosa were prepared by maceration. The antiulcer activity of ethanol extract were evaluated by using pylorus ligated and aspirin induced ulceration models. The administration of ethanol extract of ethanol extract of Ficus religiosa at the dose of 200 mg/kg and 400 mg/kg significantly decreased the ulcer index, pH, gastric volume, free acidity and total acidity compared to control group. The findings proposed the antiulcer activity of ethanol extract of Ficus religiosa fruit.

Evaluation of Anti-ulcer activity of Ethanolic Extract of Nymphaea Alba Linn Flower in experimental rats.

Evaluation of Anti-ulcer activity of Ethanolic Extract of Nymphaea Alba Linn Flower in experimental rats.

Antioxidant and antiulcer activity of Ziziphus rugosa Lam. bark

Ziziphus rugosa Lam. belongs to the family Rhamnaceae, commonly it is called as Suran in Hindi, Chunu koli in Urdu and Badara in Sanskrit and in Kannada is called as Belamarluhannu. The bark is rich in phytoconstituents like alkaloids, carbohydrates, saponins, terpenoids, phytosterols, flavanoids and tannins. Ethonolic extract of Ziziphus rugosa Lam. stem bark was evaluated for DPPH and ABTS radical scavenging activity and antiulcer activity using two models, ethanol induced gastric ulcer model and indomethacin induced gastric ulcer model in rats, treated with doses of 100mg/kg of body weight and 200mg/kg of body weight. Evaluation was done on both models comparing with reference standard ranitidine (50mg/Kg/ p. o.). The extract has shown radical scavenging effect of maximum 55.84% and 81.69% in DPPH and ABTS assays respectively. The present study provides a strong evidence of antiulcer activity of ethanolic extract against gastric lesions. The antiulcer activity is recognized by a reduction in acid secretory parameters (i.e. total and free acid), gastric volume, gastric ulcer index and percentage protection.

Peptic Ulcer Healing Activity of Fruit Pulp Extract and Seed Oil of Adansonia digitata Linn

Adansonia digitata (AD) Linn has been used to cure PU in Ayurveda but its efficacy has not been validated. The current study was so carried out to evaluate the antiulcer activity of ethanolic extract of Adansonia digitata fruit pulp (ADFP), n hexane extract of Adansonia digitata seed oil (ADSO) and their combination (ADFP+ADSO) in rats. The effect of AD on gastric ulcer in pylorus ligation induced and ethanol induced models was studied using doses [ADFO (500 mg/kg), ADSO (300 mg/kg) and combination of ADFP and ADSO] for 10 days. Omeprazole (10 mg/kg) were used as the standard drug. Depending on the model, outcomes measures were gastric volume, pH, free acidity, total acidity, ulcer index, percentage inhibition of ulcer index, protein, pepsin, mucus, antioxidant marker enzyme level (Superoxide dismutase, Catalase, Lipid peroxidation), morphological and Histopathological study. The result obtained with combination was set up near to the standard drug and consequence showed that the combination of ADFP and ADSO was found to be more effective than the individual extract of AD. The outcomes were statistically evaluated with the one-way ANOVA followed by the test of Dennett’s‘t’. The secondary-metabolites such as flavonoids, proteins, saponins, tannins, phenols, terpenoids, alkaloids and Fatty acids (palmitic acid, oleic acid, linoleic acid) are potent as antioxidant, antiulcer and anti-inflammatory. The finding of this reading confirmed that AD has antiulcer activity due to 1 or more of the secondary-metabolites present in it. Therefore, this study validates its antiulcer use in Ayurveda. Future investigation on separation of specific phytochemicals and elucidate MOA are needed.

Antiulcer activity of ethanolic extract of Sesamum Indicum on experimental animal models

Antiulcer activity of ethanolic extract of Sesamum Indicum on experimental animal models

Gastro Protective effect of Standardized Ethanolic leaf extract of Indigofera tinctoriao on experimental Gastric Ulcers in Rats

Objective:The anti-ulcer activity of ethanolic extract of Indigofera tinctoria (Fabaceae) leaf (EEIT) was investigated in Indomethacin and ethanol induced ulcer models in wistar rats. Methods: The preliminary Phytochemical evaluation was carried out by standard methods. Indomethacin and ethanol induced ulcer models the common parameter determined was ulcer index. Animals are divided in to four groups. First groups serves as control, second group treated with standard drug third and fourth group treated with EEIT at doses of 200 and 400 mg/kg p.o produced significant inhibition of the gastric lesions induced by Indomethocin induced ulcer and Ethanol induced gastric ulcer. Results: Phytochemical evaluation shows that alkaloids and flavonoids are present in extract.The extract (200 mg/kg and 400 mg/kg) showed significant (P<0.01) reduction in gastric volume, free acidity and ulcer index as compared to control. This present study indicates that Indigofera tinctoria extract have potential anti ulcer activity in the both models.Cellular level changes was reveled with histopathological studies. Conclusion:These results may further suggest that ethanolic extract was found to possess antiulcerogenic as well as ulcer healing properties, which might be due to its antisecretory activity.

EXPERIMENTAL EVALUATION OF THE ANTI-ULCER ACTIVITY OF GRAPE (VITIS VINIFERA) SEED EXTRACT IN WISTAR ALBINO RATS AGAINST HYDROCHLORIC ACID – ETHANOL INDUCED ULCER MODEL

Background:Herbal products are finding increasing demand in the treatment of Peptic ulcer, on account of their better safety and efficacy.Objectives:To evaluate the anti-ulcer activity of ethanolic extract of seeds of Vitis Vinifera in Wistar albino rats.Methods:The ethanolic extract of Vitis Vinifera was investigated for its anti-ulcer activity in rats against HCl – Ethanol induced ulcer model. The antiulcer activity was assessed by determining and comparing gastric volume, pH, free and total acidity; ulcer number and its inhibition, ulcer severity and ulcer index.Results:A significant antiulcer activity was observed. The grape seed 100mg/kg group showed significant [p &lt; 0.05] reduction in ulcer number 30.00 ± 3.23 as compared to the control. The grape seed 200mg/kg showed significant [p &lt; 0.05] reduction in ulcer number 27.33 ± 2.97, free acidity 20.00 ± 2.26, and gastric lesion18.00 ± 2.96 as compared to the control.Conclusion:This present study indicates that Vitis vinifera seed extract has potential anti ulcer activity in the model tested. Keywords: Vitis vinifera, Anti-ulcer, Ethanol, Free acidity, Total acidity

Evaluation of Antiulcer and Antioxidant Activity of Barleria gibsoniDalz. Leaves.

Background:Peptic ulcer is a digestive disorder most commonly found in clinical practice. Given the many side effects of modern medicine, the initial acquisition of fewer side effects, and medication of indigenous drugs, it should be considered as a better alternative for the treatment of peptic ulcer.Objective:To assess antiulcer and antioxidant activity of ethanol extract of Barleria gibsoni (EBG) Dalz. leaves in ulcer-induced rats and in vitro antioxidants method, respectively.Materials and Methods:Ethanol EBG was screened for antiulcer activity in pylorus ligation-induced ulcer models in Wistar rats. In vitro antioxidant activity of the extracts was tested using 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO) radical scavenging activity. Total phenol and flavonoid content in the extracts were determined spectrophotometrically.Results:Oral administration of ethanol extract of leaves at doses of 250, 500 mg/kg p.o. reduced significant gastric lesions induced by pylorus ligation-induced ulcer as compared to standard omeprazole (20 mg/kg p.o.). The IC50 values were found to be 150 μg/mL in leaves extract. The ethanol extracts showed good antioxidant capacity in DPPH radical scavenging assay and NO radical scavenging activity when compared to standard. The total phenolic content using Folin–Ciocalteu reagent estimated in 1 mg of leaves extracts was 368 μg and 481 μg with gallic acid equivalent and also the total flavonoid content found to be 240 and 410 μg, respectively, with quercetin equivalence.Conclusion:These findings suggest that the leaves of B. gibsoni possessed antiulcer potential and antioxidant compared to standard. This is the first ever report of antiulcer and antioxidant activities in B. gibsoni (Acanthaceae).SUMMARY In vivo antiulcer and in vitro antioxidant activity of Barleria gibsoni was evaluated.Soxhelt extraction was carried out and extracts were subjected to qualitative phytochemical analysis. Extract obtained by Soxhlation showed higher total phenolic and flavonoid contents.EBG showed DPPH and Nitric oxide scavenging activity indicating its strong antioxidant potential.On pylorus ligation-accumulated secretions and the related ulcers confirm gastric acid output to be the basic cause of gastric ulcers. Ethanol extract of leaves attenuated the gastric volume, free acidity, total acidity and ulcer index thus showing the anti-secretory mechanism.The results of the histopathological investigation of Barleria gibsoni leaves for antiulcer effects using pylorus ligation induced ulcer model in rats laid credence to traditional use of the plant leaves in ulcer treatment. The ethanol extract of leaves demonstrated increase in percentage preventive index compared to omeprazole respectively. From the present study results reveals the antiulcer activity of ethanol extract leaves which is comparable to that of Omeprazole. Abbreviations Used: EBG: Ethanol extract prepared from the leaves of B. gibsoni, ROS: Reactive Oxygen Species, DPPH: 2, 2-diphenyl-1-picrylhydrazyl, NO: Nitric Oxide, IC50: The half maximal inhibitory concentration, m: mg, DNA: deoxyribonucleic acid, GAE: Gallic acid equivalence, AlCl3: Aluminium chloride

Experimental evaluation of the anti-ulcer activity of the ethanolic extract of grape (Vitis vinifera) seed in wistar albino rats against aspirin plus pylorus ligation induced gastric ulcer model

Background: There is an increased demand for newer safer drugs for the treatment of peptic ulcer disease as its incidence is increasing gradually in view of changing lifestyle and stress. The objective of this study was to evaluate the anti-ulcer activity of ethanol extract of seeds of Vitis vinifera. Methods: The ethanol extract of Vitis vinifera was investigated for its anti-ulcer activity in rats against Aspirin plus Pylorus ligation induced gastric ulcer. The antiulcer activity was assessed by determining and comparing gastric volume, pH, free and total acidity; ulcer number and its inhibition, ulcer severity and ulcer index. Results: A significant antiulcer activity was observed. Pylorus ligation model showed significant (p<0.01) reduction in gastric volume, free acidity and ulcer index as compared to control. Conclusions: This present study indicates that Vitis vinifera seed extract have potential anti-ulcer activity in the model tested.

Antiulcer Activity of Ethanolic Extract of Salvadora indica (W.) Leaves on Albino Rats.

Ulcer can be developed inside the inner lining of the stomach (gastric ulcer) or the small intestine (duodenal ulcer). Both the ulcers are also cumulatively referred as peptic ulcers. It affects nearly 10% of world population. To investigate the antiulcer activity of ethanolic extract of Salvadora indica W. leaves (ESIL) on albino rats. The present study was carried by pylorus ligation, ethanol and cysteamine induced ulcer models in albino rats. The antiulcer activity of ESIL (150, 300 and 600 mg/kg p.o. for 7 days) was compared with standard drugs (Ranitidine). In pyloric ligation induced ulcer model, the studied parameters were gastric volume, pH, total acidity, free acidity, and ulcer index whereas in ethanol and cysteamine induced ulcer model, the ulcer index was determined for severity of ulcers. The parameters studied were ulcer index, gastric juice volume, pH, free acidity and total acidity. In pyloric ligation model; the volume of gastric content, total/free acidity and pepsin activity was significantly decreased at p<0.05 and p<0.01 and pH of the gastric juice was significantly increased at p<0.05 and p<0.01 in ESIL treated groups as compared to control group. All the doses of ESIL showed dose dependent antiulcer effect as well as significant (p<0.05 and p<0.01) reduction in the ulcer index as compared to control group in all the experimental models. The results of the study indicate that the ESIL have better potential against ulcer which supports the traditional claims in folklore medicine.

PHARMACOLOGICAL EVALUATION OF ANTI-ULCER ACTIVITY OF Cymbopogon flexuosus

Background: Peptic ulcer disease is a worldwide problem. Currently, there is no cost-effective treatment to relieve pain, heal the ulcer and prevent ulcer recurrence. Hence, there is a dire need to search and find a suitable treatment from natural sources. The present study was designed to investigate the anti-ulcer activity of ethanol extract of the leaves of Cymbopogon flexuosus. Methods: The ethanol extract of the leaves of Cymbopogon flexuosus was prepared by hot extraction method. Anti-ulcer activity was evaluated in rats and method employed was pylorus ligation. Animals were divided into four groups of six animals each. The animals of Group I served as normal control (vehicle) which received normal saline (5 ml/kg b.wt., p.o). Group II and III received 200 and 400 mg/kg b.wt. of ethanol extract, respectively. The animals of Group IV served as standard control which received ranitidine (15 mg/kg bd.wt.). At the end of study parameters like ulcer index, free acidity, total acidity, acid volume, and pH were determined. Results: The ethanol extract showed a significant reduction in the total acidity, free acidity, and acid volume. The efficacy of plant extract at high dose was comparable with the standard drug - ranitidine. Conclusion: Our study results support the ethnomedical use of leaves of C. flexuosus .

Antiulcer activity of the ethanolic extract and ethyl acetate fraction of the leaves of Markhamia tomentosa in rats

Ethnopharmacological relevanceThe leaves of Markhamia tomentosa (Benth.) K. Schum. Ex Engl. (Bignoniaceae) are used traditionally in the treatment of skin afflictions, sores, ulcers and inflammation. The aim of the study was to investigate the antiulcer activity of the crude ethanolic extract from the leaves of Markhamia tomentosa, determine the active fraction(s) of the extract and identify the chemical constituents in the active fraction by LC–MS. Materials and methodsThe antiulcer activity of the crude extract (50, 100 and 150mg/kg, p.o.) was evaluated in ethanol and indomethacin-induced models while the solvent fractions (150mg/kg) were screened using ethanol-induced gastric lesions in rats. Furthermore, anti-ulcer activity of the active fraction (50, 100 and 150mg/kg, p.o.) was performed using indomethacin and pylorus ligation models. Parameters such as gastric volume, pH and acidity were determined in the pylorus ligation model. LC–ESI–MS analysis was used to identify the components in the active fraction. ResultsThe extract at the dose of 50, 100 and 150mg/kg caused a significant (p<0.05) dose-dependent inhibition of ulcer in the ethanol and indomethacin-induced ulcer models, respectively. The ethyl acetate (EtOAc) fraction showed the most potent antiulcer activity from all the fractions tested. This fraction produced 72% and 92% inhibition of indomethacin and pylorus-induced ulcer at a dose of 150mg/kg respectively. Acteoside, luteolin, luteolin-7-rutinoside, Luteolin-3′,7-di-O-glucoside, carnosol, dilapachone, tormentic acid, oxo-pomolic acid and ajugol were detected in the EtOAc fraction. ConclusionOur data provide a rational base for the folkloric use of Markhamia tormentosa in the treatment of ulcers.

Antiulcer activity of ethanolic extract of Markhamia tomentosa leaves in rats

Antiulcer activity of ethanolic extract of Markhamia tomentosa leaves in rats

Retraction Note : Evaluation of Antiulcer Activity of Ethanolic Extract of Momordica Dioca on Pylorus Ligature Induced and Aspirin Induced Ulcer in Albino Rats

Asian Journal of Biomedical and Pharmaceutical Sciences Retraction Note Retraction Note: Evaluation of Antiulcer Activity of Ethanolic Extract of Momordica dioca on Pylorus Ligature Induced and Aspirin Induced Ulcer in Albino Rats Anjaly Mary Verghes 1* Naveen kumar .Z 2 1 Department of Pharmacology. Santhiram Medical College. Nandyal .A.P. 2 Department of Physiology, Santhiram Medical College. Nandyal. A.P. Anjaly Mary Verghes Email: anjalianjali82@rediffmail.com Published online: 1 5 th September 2013 Without Abstract The online version of the original article can be found at http://www.jbiopharm.com/index.php/ajbps/article/view/364 Retraction to: Asian Journal of Biomedical and Pharmaceutical Sciences (2013) 3(23):49-52. The Editors and publisher regret to report that the paper published by Anjaly Mary Verghes and Naveen kumar Z, “ Evaluation of Antiulcer Activity of Ethanolic Extract of Momordica dioca on Pylorus Ligature Induced and Aspirin Induced Ulcer in Albino Rats ” in Asian Journal of Biomedical and Pharmaceutical Sciences (2013) 3(23):49-52, is retracted due to some unavoidable circumstances”. Pradeep T Deshmukh Executive Editor

Evaluation of antiulcer activity of ethanolic extract ofMomordica dioicaon pylorus ligature induced and ibuprofen induced ulcer in albino rats

Objective: The aim of the present study is to evaluate the anti -ulcer activity of Ethanolic extract of Momordica Dioica in pylorus ligation and ibuprofen induced gastric ulcers in rats. Methods: Gastric ulcer was induced by giving ibuprofen (200mg/Kg) and by pylorus ligation method. The animals used for the experiment were divided into 4 groups for each model, 6 rats in each group. In pylorus ligation model, all groups of rats were pre -treated with test drugs, Group -I (control) received 2%gum acacia- 2ml/100g, Group-II (standard) received Ranitidine (60mg/kg.) and group -III, IV were treated with Ethanolic extracts 150mg/kg, 300mg/kg. respectively orally 30mins prior to pylorus ligation. The Antiulcer activity of Momordica Dioica was assessed by determining and comparing gastric volume, free acidity, total acidity, pH , percentage of ulcer protection, ulcer index. In ibuprofen induced ulcer model, all groups of rats were treated with test drugs for 7 days prior to ibuprofen induced ulcer. Animals were divided into 4 groups and treated with drugs as in above model. After 7 days of treatment, animals were fasted for 24 hrs. Ulcers were produced by giving ibuprofen (200mg/Kg) on the day of sacrifice. The animals were sacrificed 4 hours later and stomachs were open along the greater curvature and ulcers were graded. Percentage of ulcer protection, ulcer index were obser ved and calculated. Results: The extract of Momordica Dioica in pylorus ligation model, it decreased the ulcer index (1.66) and there was a decrease in total gastric acid and free acid (p<0.0001), and increases the pH value (p<0.0001) and also reduces the total gastric volume (p<0.0003), increases the percentage of ulcer protection (61.66%). In ibuprofen induced ulcer model, it decreases the ulcer index (10.66) and increases the ulcer protection (72.09%). Conclusion: The Ethanolic extract of Momordica Dioica was clearly shows a protective effect against total acid, free acid, gastric volume and ulcer index and also increases pH and percentage of protection against ulcers in both models.