Antioxidant Profile Research Articles

Cytotoxic and Antioxidant Properties and Profile of Active Compounds in Kale and Lupine Sprouts Supplemented with γ-Polyglutamic Acid During Sprouting

γ-Polyglutamic acid (γ-PGA) is a biodegradable and non-toxic biopolymer with numerous potential applications in agriculture, food, and health sciences due to its antioxidant and antimicrobial properties. This study evaluated the cytotoxic and antioxidant properties of kale (Brassica oleracea var. sabellica) and lupine (Lupinus luteus) sprouts supplemented with different concentrations of γ-PGA. The sprouts were cultivated for various durations (6–10 days), and their bioactive compound profiles were analyzed using HPLC with a PDA 100 UV-VIS detector. Antioxidant activity was assessed via DPPH and FRAP assays, while cytotoxicity was tested against cancer and normal colon cell lines. Results demonstrated that kale sprouts had significantly higher antioxidant activity compared to lupine, with the highest levels observed in kale sprouts supplemented with 0.01% γ-PGA on day 8 (DPPH: 63.6 ± 0.4 μM TEAC/g dw; FRAP: 181.8 ± 2.1 μM/Fe2+/g dw). In contrast, supplementation of lupine sprouts with γ-PGA showed mixed effects, with antioxidant activity depending on concentration and cultivation duration (DPPH in the range 6.5 ± 0.2 ÷ 12.4 ± 0.2 μM TEAC/g dw; FRAP in the range 14.3 ± 0.4 ÷ 25.2 ± 0.9 μM/Fe2+/g dw). Cytotoxicity assays revealed that neither kale nor lupine extracts were toxic to normal colon cells (approx. 100% of alive cells), suggesting selectivity in their action, but fortification with γ-PGA resulted in a weaker or even unfavorable effect on the cytotoxic activity of the examined sprouts. The findings highlight the potential of γ-PGA to enhance the bioactive properties of sprouts, although its effects are influenced by species and cultivation conditions. These results provide a foundation for developing functional foods and sustainable agricultural practices.

Exploring two bioreactor systems for micropropagation of Vaccinium membranaceum and the antioxidant enzyme profiling in tissue culture-raised plants

Abstract Vaccinium membranaceum (black huckleberry) is known for its high content of bioactive compounds. This study introduces a novel approach for bioreactor micropropagation using stationary (Growtek) and temporary immersion (RITA) bioreactor systems using a liquid nutrient medium to enhance the in vitro propagation of black huckleberry. Adventitious shoot regeneration from leaf explants reached 80% efficiency in a semi-solid nutrient medium with 10 μM thidiazuron. Shoot proliferation was optimized in liquid culture, where the Growtek system yielded the most robust shoot regeneration at 76% and second highest was observed on RITA at 40.33%. Morphological and histological analyses of different cultured explants revealed various stomatal density, trichome formation and mesophyll tissue organization. Biochemical profiling of antioxidant enzymes showed that greenhouse grown leaves had higher levels of bioactive compounds, such as flavonoids, proanthocyanins, and antioxidant activity. However, Growtek-cultured leaves exhibited elevated phenolic content and catalase activity. Additionally, antioxidant enzymes, including catalase (2.8 U g−1 min−1 flw) and superoxide dismutase (0.80 U g−1 min−1 flw), were higher in Growtek and leaf-cultured shoots, respectively. The greenhouse grown plants demonstrated the highest overall phytochemical activity, with the most abundant proanthocyanins (8.3 mg g−1 flw) and anthocyanins (26 mg g−1 flw). This work presents a highly efficient liquid micropropagation protocol for black huckleberry with insights into its bioactive compounds and antioxidant enzyme profiles, highlighting its potential in commercial production and medicinal applications.

Mustard lung with a unique oxidative stress profile as an independent pulmonary disease.

Mustard lung with a unique oxidative stress profile as an independent pulmonary disease.

Productive performance, rumen parameters, carcass quality, antioxidant profile and methane emission in lambs supplemented with triticale hay.

Productive performance, rumen parameters, carcass quality, antioxidant profile and methane emission in lambs supplemented with triticale hay.

Design, Synthesis and Characterization of New Amide-Linked Chromone-Isoxazole Hybrids: In Vitro Anti-Bacterial and Antioxidant Evaluation, DFT Calculations, ADMET Profiling, Docking and Molecular dynamics simulation

Design, Synthesis and Characterization of New Amide-Linked Chromone-Isoxazole Hybrids: In Vitro Anti-Bacterial and Antioxidant Evaluation, DFT Calculations, ADMET Profiling, Docking and Molecular dynamics simulation

Changes in antioxidant and flavor profiles of raspberry, blackberry, and mulberry juices fermented by urolithin A-producing Limosilactobacillus fermentum FUA033

Changes in antioxidant and flavor profiles of raspberry, blackberry, and mulberry juices fermented by urolithin A-producing Limosilactobacillus fermentum FUA033

A Proprietary Punica granatum pericarp Extract, Its Antioxidant Properties Using Multi-Radical Assays and Protection Against UVA-Induced Damages in a Reconstructed Human Skin Model

Background: Within the solar ultraviolet (UV) spectrum, ultraviolet A rays (UVA, 320–400 nm), although less energetic than ultraviolet B rays (UVB, 280–320 nm), constitute at least 95% of solar UV radiation that penetrates deep into the skin The UV rays are associated with both epidermal and dermal damage resulting from the generation of reactive oxygen species (ROS). Among them, the longest UVA wavelengths (UVA1, 340–400 nm) can represent up to 75% of the total UV energy. Therefore, UVA radiation is linked to various acute and chronic conditions, including increased skin pigmentation and photoaging. Despite many advances in the skin photoprotection category, there is still a growing demand for natural daily photoprotection active ingredients that offer broad protection against skin damage caused by UVA exposure. In our quest to discover new, disruptive, next generation of photoprotective ingredients, we were drawn to pomegranate, based on its diverse polyphenolic profile. We investigated the pericarp of the fruit, so far considered as byproducts of the pomegranate supply chain, to design a novel patented extract “POMAOX” with a desired spectrum of phenolic components comprising of αβ-punicalagins, αβ-punicalins and ellagic acid. Methods: Antioxidant properties of POMAOX were measured using in-tubo standard tests capable of revealing a battery of radical oxygen species (ROS): peroxyl radical (ORAC), singlet oxygen (SOAC), superoxide anion (SORAC), peroxynitrite (NORAC), and hydroxyl radical (HORAC). In vitro, confirmation of antioxidant properties was first performed by evaluating protection against UVA-induced lipid peroxidation in human dermal fibroblasts (HDF), via the release of 8 iso-prostanes. The protection offered by POMAOX was further validated in a 3D in vitro reconstructed T-SkinTM model, by analyzing tissue viability/morphology and measuring the release of Matrix Metallopeptidase 1 (MMP-1) & pro-inflammatory mediators (IL-1α, IL-1ra, IL-6, IL-8, GM-CSF, and TNF-α) after UVA1 exposure. Results: POMAOX displayed strong antioxidant activity against peroxynitrite (NORAC) at 1.0–3.0 ppm, comparable to the reference vitaminC, as well as singlet oxygen (SOAC) at 220 ppm, and superoxide radicals with a SORAC value of 500 ppm. Additionally, POMAOX demonstrated strong photoprotection benefit at 0.001% concentration, offering up to 74% protection against UVA-induced lipid peroxidation on HDF, in a similar range as the positive reference, Vitamin E at 0.002% (50 µM), and with higher efficacy than ellagic acid alone at 5 µM. Moreover, our pomegranate-derived extract delivered photoprotection at 0.001%, mitigating dermal damages induced by UVA1, through inhibition of MMP-1 and significant inhibition of pro-inflammatory mediators release (including IL-1α, IL-1ra, IL-6, IL-8, GM-CSF, and TNFα) on an in vitro reconstructed full-thickness human skin model with a similar level of protection to that of Vitamin C tested at 0.035% (200 µM). Conclusions: Overall, the novel pomegranate-derived extract “POMAOX” significantly reduced the impact of UVA on human skin, due to its broad-spectrum antioxidant profile. These findings suggest that POMAOX could offer enhanced protection against the detrimental effects of UV exposure, addressing the growing consumer demand for strong photoprotection with skincare benefits.

Screening for Antioxidant Effects and Chemical Profiles of Different Extracts of Rheum ribes Parts.

Rheum ribes L. (rhubarb) is a valuable plant that is consumed both raw and cooked and contains important plant secondary metabolites. The aim of this study was to investigate the chemical profile and biological activities of different extracts of aerial parts and roots of R. ribes. The total phenolics and flavonoids content was changed by the extraction solvents and the highest total phenolics contents of both parts were found in the methanol extract (root: 140.97 mg GAE/g; aerial parts: 74.56 mg GAE/g). In the chemical profile, it was found that hesperidin is the main component in the extracts of the aerial part (613.48-12194.83 µg/g of extract), while in the root extracts the dominant components are catechin (799.11-3230.44 µg/g extract) and gallic acid were (184.03-960.58 µg/g extract). The best radical scavenging and reducing ability was provided by the methanol extract of the roots, while the methanol extract of the aerial parts had the greatest metal chelating ability (62.05 mg EDTAE/g). The best AChE and BChE abilities were determined in the methanol extracts of both parts. Our results suggest that R. ribes can be considered as a valuable source of bioactive compounds for the development of health-promoting applications.

Changes in Oxidative Stress, Inflammatory Markers, and Lipid Profile After a 6-Week High-Antioxidant-Capacity Dietary Intervention in CVD Patients.

Background/Objectives: Increased dietary antioxidant capacity is a good means of lowering oxidative stress and cardiovascular risk. Established antioxidant capacity doses should be tested using dietary intervention. Methods: We analysed the influence of a high-antioxidant-capacity diet on oxidative stress (OS) and inflammatory and lipid profile in CVD (cardiovascular disease) subjects with initially low (LowA) and high (HighA) antioxidant capacity markers. It was an experimental study with a 6-week dietary intervention (DI). Forty-eight CVD patients completed the DI. Blood and urine samples were collected, and anthropometric measurements were taken. Dietary data were collected using a multi-day food record method. α-tocopherol, β-carotene, and retinol were chosen as antioxidant capacity markers; F2-isoprostanes (F2-IsoP), oxidised low-density lipoproteins (oxLDL), and uric acid (UA) were used as OS markers; and interleukin 6 (IL-6) and high-sensitivity C-reactive proteins (hs-CRP) were used as inflammatory markers. Total cholesterol, low- and high-density lipoproteins, and triglycerides (TCHOL, LDL, HDL, TRI) as lipid profiles were analysed. Two groups of subjects with LowA and HighA profiles were identified. Results: The total dietary antioxidant capacity intake during DI was increased by 56%. In the total sample, the DI increased β-carotene, retinol, and UA, and decreased IL-6 oxLDL. The LowA group exhibited increased β-carotene, α-tocopherol, retinol, and decreased IL-6. The HighA group exhibited increased β-carotene and decreased IL-6, F2-IsoP, oxLDL, and oxLDL/LDL ratio. In the HighA group, compared to the LowA group, greater decreases in α-tocopherol and F2-IsoP were found. In both groups, inflammatory markers (IL-6) decreased, and β-carotene increased. Conclusions: The DI results depended on the antioxidant capacity profile at baseline; nevertheless, the established DI including selected antioxidative snacks significantly decrease oxidative stress and improve antioxidant capacity. Further research on diet natural antioxidant supplementation needs to be continued.

Growth of Spirulina spp. at different temperatures and their impact on pigment production, oxidants and antioxidants profile.

The study investigated the effect of different temperatures on the growth, pigment production, oxidative stress markers, and antioxidant profile of Spirulina spp. The results showed that the maximum growth of Spirulina was observed at 20°C, while the chlorophyll a, chlorophyll b, and carotenoid contents decreased at higher temperatures. Additionally, the concentration of oxidants such as MDA and H2O2 increased with temperature, while the concentration of antioxidants such as SOD, POD, and APX also increased with temperature. However, the optimal concentration of antioxidants varied with temperature, with the highest concentration of SOD and POD observed at 30°C and the highest concentration of CAT and APX observed at 20°C. Overall, the study suggests that the growth of Spirulina is temperature-dependent, with optimal growth observed at 20°C. Additionally, the study highlights the complex relationship between temperature and oxidative stress in Spirulina, with both oxidants and antioxidants increasing with temperature. However, the optimal concentration of antioxidants varies with temperature, indicating the need for further research to understand the mechanisms underlying these observations. The findings of this study have implications for the cultivation of Spirulina, particularly in regions where temperatures fluctuate significantly, and may also have implications for the use of Spirulina as a dietary supplement.

Low Serum and Urine Fetuin-A Levels and High Composite Dietary Antioxidant Index as Risk Factors for Kidney Stone Formation.

Background: Fetuin-A prevents the precipitation of hydroxyapatite in supersaturated solutions of calcium and phosphate; however, its relationship with nephrolithiasis has yet to be clarified. The aim of this study was to investigate the protective and predictive roles of serum and urine fetuin-A levels in nephrolithiasis and their relationships with the composite dietary antioxidant index (CDAI). Methods: This study involved 75 adult patients with kidney stone disease and 71 healthy adults without kidney stone disease in the control group. Participants had specific anthropometric measurements taken, and three-day food records were kept. The CDAI was calculated by summing six standard antioxidants, including vitamins A, C, and E, manganese, selenium, and zinc, representing participants' antioxidant profile. In addition to some analyzed serum and urine parameters of the participants, fetuin-A levels were measured using the enzyme-linked immunosorbent assay (ELISA) method. Results: In patients with kidney stones, both serum and urine fetuin-A levels (676.3 ± 160.14 ng/mL; 166.6 ± 128.13 ng/mL, respectively) were lower than in the control group (1455.6 ± 420.52 ng/mL; 2267.5 ± 1536.78 ng/mL, respectively) (p < 0.00001). In contrast, the CDAI was higher in patients with kidney stones compared to those without kidney stones (p < 0.001). Besides, several dietary parameters had significant positive correlations with serum and/or urinary fetuin-A. Conclusions: The present study suggests that serum and urinary fetuin-A levels may serve as protective factors against kidney stones and could potentially be used as predictive markers for the development of nephrolithiasis. Furthermore, our results suggest that the CDAI above a certain level may increase the risk of stone formation and that some dietary parameters may affect the levels of this biomarker in serum and urine.

Dark septate endophytic fungus Alternaria sp. 17463 regulates various antioxidant enzymes and compounds to mitigate salt stress caused by different anion salts.

Dark septate endophytic fungus Alternaria sp. 17463 regulates various antioxidant enzymes and compounds to mitigate salt stress caused by different anion salts.

The Toxicological Impact of the Ultraviolet Filter Oxybenzone on Antioxidant Profiles in In Vitro Cultures of Lentinula edodes

A detailed understanding of the toxic effects of organic UV filters, such as oxybenzone, on living organisms is crucial for assessing the feasibility of bioremediation methods. Due to the widespread use of oxybenzone as an ultraviolet filter in sunscreens, it has become an emerging contaminant of concern in the environment. This concern extends to fungi, which have the potential to neutralize a wide variety of xenobiotics released into the environment. The primary objective of the study was to elucidate the alterations of antioxidant profiles of the white-rot fungus Lentinula edodes in response to oxybenzone exposure. Samples with oxybenzone at a final concentration of 0.1 mg mL−1 were cultured in vitro with the mycelium of L. edodes for 14 days. The contents of the following antioxidant compounds were assessed: indole derivatives (6-methyl-D,L-tryptophan, tryptophan), ergothioneine, and phenolic acid (p-hydroxybenzoic acid), as well as lovastatin and ergosterol. The addition of oxybenzone negatively affected biomass growth, reducing it from 3.205 ± 0.4022 g to 0.5803 ± 0.1019 g. A considerable reduction in oxybenzone amounts was found in the medium after incubation (from 25 mg to 0.2993 ± 0.1934 mg). After lyophilization, the mycelium contained 1.1591 ± 0.0323 mg of oxybenzone. Additionally, eleven biotransformation products were assessed in the mycelium and medium samples using UPLC-Q ToF. After incubation, the transformation products were identified based on monoisotopic molecular mass and fragmentation spectra. The observed increase in the content of some antioxidants, e.g., ergothioneine, while reducing the content of others, such as lovastatin, suggests that the impact of xenobiotics on the antioxidant profile of in vitro cultures of L. edodes is complex. Marked alterations in biomass growth suggest a potential toxicological risk associated with oxybenzone. This study contributes to the understanding of the environmental impact of UV filters and emphasizes the need for safer alternatives.

Physicochemical, microbiology, and sensory characteristics of kombucha prepared with Tommy mango peel flour.

Fermented foods are a good option due to the beneficial compounds generated in the fermentation process and the low pH that allows conservation without additives. The objective of the study was to produce and include Tommy mango peel flour in the production of kombucha and evaluate its effects on the physicochemical and sensory properties, antioxidant capacity, and microbiological profile. The kombucha was developed with green tea and the addition of Tommy mango peel flour (10% and 20%). The kombuchas were evaluated in the first fermentation (aerobic) and at the end of the second fermentation (anaerobic), the granulometry and colorimetry of the flour and the antioxidant profile were evaluated. Microbiome analysis was performed by 16S DNA extraction. For sensory analysis, an affective test was performed for global evaluation, flavor, texture, and oral perception. The results showed that the total phenolic content was 4.86 mg EAG/mL in F1, 8.79 mg EAG/mL in F2 with 10% mango peel flour, and 8.83 ± 0.54 mg EAG/mL in F2 with 20% mango peel flour, evidencing a significant increase in the second fermentation with the addition of the flour. In addition, the antioxidant activity was also higher in the second fermentation. The values obtained were F1 = 15.27 µmol TE/mL; F2 with 10% FCMT = 18.80 µmol TE/mL; and F2 with 20% FCMT = 26.76 µmol TE/mL. These findings indicate that the antioxidant capacity increases significantly during the second fermentation, directly correlating with the amount of mango peel flour added. The most abundant bacterial genera were Liquorilactobacillus nagelii (72%), Acetobacter (13%), and Komagataeibacter (12%) and for fungi (90%) Brettanomyces/Dekkera bruxellensis. The beverage obtained different levels of acceptance among consumers and non-consumers only in terms of flavor, proving to be a good alternative for the food industry for applying a mango byproduct to drinks.

Enhancing Commercial Gourmet Oil Quality: The Role of Dried Cayenne Pepper Red (Capsicum annuum L.) as a Natural Additive.

This study assessed the potential of dried Cayenne pepper (CP; Capsicum annuum L.) as a natural additive to rice bran oil (RBO), grape seed oil (GSO), and virgin olive oil (OO). Key analyses included peroxide and acid values, oxidative stability (Rancimat method), the composition of fatty acids (FAs) (GC-FID method), antioxidant activity (AA; DPPH method), and antimicrobial properties (disc diffusion method). Capsaicin and the dihydrocapsaicin contents in CP were quantified (HPLC-DAD method) as 1499.37 ± 3.64 and 1449.04 ± 5.14 mg/kg DW, respectively. Oleic acid (C18:1cis n9) dominated in OO (69.70%), OO-CP (69.73%), and RBO-CP (38.97%), while linoleic acid (C18:2cis n6) prevailed in RBO (41.34%), GSO (57.93%), and GSO-CP (58.03%). The addition of CP influenced the FA profile, particularly linoleic acid in OO and RBO, and all FAs in GSO. Peroxide and acid values increased significantly in RBO and GSO upon CP addition, but induction times remained unaffected. The strongest AA (77.00 ± 0.13%) was observed in OO-CP. Cayenne pepper significantly enhanced the antioxidant profiles of all oils compared to the counterparts. However, the antimicrobial activity was weak (≤5.0 mm inhibition zones) against tested microorganisms. These findings support CP as a functional additive for enhancing the nutritional and functional properties of gourmet oils, while highlighting the need for further optimization to improve stability and bioactivity.

Endogenous Ketone Bodies Are Associated with Metabolic Vulnerability and Disability in Multiple Sclerosis.

Purpose: Ketone bodies could be useful biomarkers in multiple sclerosis (MS) because the pathophysiological processes underlying MS disease progression induce metabolic stress. The purpose was to assess the relationships of ketone bodies with biomarkers of metabolic, inflammatory, and oxidative stress in MS. Methods: Blood samples and neurological assessments were obtained from 153 healthy controls (HC), 187 relapsing-remitting (RRMS), and 91 progressive MS (PMS) patients. AcAc, BHB, and acetone were measured using proton nuclear magnetic resonance spectroscopy. Indices of inflammatory vulnerability (IVX), metabolic malnutrition (MMX), and metabolic vulnerability (MVX) were computed from the NMR profiles. Cholesterol, apolipoprotein, lipid peroxidation, and antioxidant profiles were obtained. Regression analysis adjusted for age, sex, body mass index, and HC, RRMS, or PMS disease status. Results: AcAc and BHB levels were greater in MS compared to HC. BHB and ketone bodies were positively associated with disability on the MS Severity Scale and ambulation time. BHB was positively associated with IVX, MMX, and MVX. AcAc was positively associated with MMX and negatively associated with IVX and MVX. Total ketone body concentration was positively associated with MMX and MVX. BHB and AcAc levels were negatively associated with the amino acids alanine, valine, and leucine. Conclusions: Ketone bodies are associated with inflammatory vulnerability, metabolic vulnerability, and ambulatory disability measures in MS.

Bioactive Compounds and Antioxidant Efficacy of Djulis (Chenopodium formosanum) Leaves: Implications for Sustainable Cosmeceutical Development.

Chenopodium formosanum (djulis), well known for its vivid color variation during diverse senescence stages, has attracted attention for its perceived health benefits and antioxidant capacity. Djulis leaves, often discarded as biowaste after harvesting, were evaluated for their potential as a source of antioxidant compounds. The current study analyzes the physicochemical and antioxidant activities of red, green, and yellow djulis leaf extracts across various senescence stages to probe their prospective utility in cosmeceuticals. Various plant compounds including total carbohydrates, phenolics and flavonoids, chlorophyll a and b, and betaxanthins and betacyanins were measured using spectrophotometric techniques. Antioxidant potential was assessed using DPPH, FRAP, CUPRAC, TEAC, and DMPD assays. Green djulis leaves displayed elevated total carbohydrate and chlorophyll levels, whereas red djulis leaves exhibited heightened phenolic, flavonoid, betaxanthin, and betacyanin content, indicating its suitability for cosmeceutical applications. Antioxidant evaluations revealed substantial disparities among the extracts, with red djulis leaf extract demonstrating superior antioxidant activity in most assays. These findings revealed the distinct antioxidant profiles of djulis leaf extracts influenced by color and senescence stage. These findings advance our understanding of the bioactive attributes of djulis leaves and their potential for incorporation into functional products.

Plasma from hypertensive pregnancy patients induce endothelial dysfunction even under atheroprotective shear stress

Preeclampsia (PE) is a challenge in maternal healthcare due to its complex nature, characterized by high blood pressure, protein in the urine, and damage to various organs. There is evidence linking PE to endothelial dysfunction (ED), triggered by substances released from an oxygen-deprived placenta. Previous in vitro studies have not considered the impact of in vivo elements, such as the different patterns of blood flow, and laminar (LSS) vs. oscillatory (OSS) shear stress, on the development of ED. We investigated the impact of plasma from healthy pregnant women (HP), subjects with gestational hypertension (GH), and PE patients on global gene expression of human coronary endothelial cells (HCAECs) under LSS and OSS. Our findings revealed a unique transcriptional profile of endothelial cells induced by plasma incubation in LSS. Notably, OSS resulted in similar transcriptomes irrespective of plasma treatment. Under LSS, GH plasma resulted in a proliferative profile, whereas PE plasma was linked to pro-inflammatory and antioxidant profiles compared to HP plasma. Our findings demonstrate that shear stress levels influence the endothelial cell transcriptome in response to plasma from hypertensive pregnancy patients. Both PE and GH can induce endothelial dysfunction under atheroprotective LSS, with a more significant effect observed with PE-derived plasma.

Individual Genomic Loci, Transcript Level and Serum Profile of Immune, Antioxidant and Hormonal Markers Associated with Sheep Arthritis.

Arthritis is a leading cause of economic loss in livestock farming including sheep. This study examined the changes in gene expression, antioxidants, pro-inflammatory cytokines, acute-phase proteins (APPs), hormonal assays and iron profiles linked to sheep arthritis, as well as the diagnostic utility of these markers. Blood samples were obtained from 30 apparently healthy rams and 30 rams with arthritis for gene expression and biochemical analyses. Gene expression intensities were much higher in the arthritis-affected rams than in the healthy ones for the genes IL-1α, IL-1β, IL-6, IL-10, TNFα, NCF4, NFKB, TMED, FCAMR, iNOS and COX18. The SOD3, CAT, GPX and ATOX1 genes were expressed at substantially lower levels in arthritis-affected rams. Disparities in the nucleotide sequence variants for the amplified DNA bases linked to arthritis for the studied genes were found in the PCR-DNA sequence verdicts of the affected and healthy rams. Immunological, acute-phase protein (APP), antioxidant, hormonal and iron profiles were estimated in both groups and statistically analyzed. The arthritic group in relation to the healthy one showed a significant (p < 0.05) increase in pro-inflammatory cytokines, APPs, free radicals, immunoglobulins, cortisol, GH, TSH, ferritin, TIBC and UIBC and a significant (p ˂ 0.05) decrease in anti-inflammatory cytokines, antioxidants, complements, insulin, T3, T4, SI, and Tf and Tf sat.% serum levels. The estimated pro-inflammatory cytokines and APPs achieved high values of sensitivity and specificity, positive predictive values (PPVs), negative predictive values (NPVs), a high accuracy rate and a moderate likelihood ratio (LR). The study concluded that ovine arthritis stimulates innate and humeral immunity, resulting in prominent alterations in gene expression, pro-inflammatory cytokines, APP assays and antioxidant profiles, which could be valuable indicators of sheep arthritis.

Lactic acid fermentation of a novel Sea buckthorn-monk fruit beverage: Phenolic profile, volatile compounds, and antidiabetic and antihypertensive potential.

Lactic acid fermentation of a novel Sea buckthorn-monk fruit beverage: Phenolic profile, volatile compounds, and antidiabetic and antihypertensive potential.