Ocean warming (OW) and freshening influence metabolism and cellular damage in Loxechinus albus at the extremes of their Patagonian distribution.

A needle-like MOFzyme with high aspect ratio and extended pH/temperature working ranges was developed for quantification of total antioxidant capacity (TAC) and oxidant-/light-free dye degradation. The MOFzyme showed a length, width, and high aspect ratio of 17 ± 8 µm, 1.5 ± 0.6 µm, and 11.33, respectively. A superb oxidase-like activity, an enhanced Vmax of 0.14 nM s-1, an improved Km of 0.12 mM, a wide pH range of 3.0-8.0, and an extended thermal range of 25-70°C were obtained, revealing their practical applicability for developing pH- and temperature-independent protocols. The MOFzyme was then utilized for the determination of TAC using trolox as a model antioxidant, providing a linear range of 0.0-200.0 μM, and a very low detection limit of 1.0 μM was obtained for TAC quantification. The selectivity studies showed that other coexisting materials cannot interfere with sensor response. The intra-day and inter-day reproducibility assays showed a %RSD as low as 1.7% and 2.36% for the sensor. The dye degradation was performed using rhodamine B as a model molecule, revealing 99.7% degradation within 40.0 min without any oxidant/light irradiation and 10 cycles of reusability. The applicability of the MOFzyme for real food and sustainable environmental applications was proved.

Cornus mas L. (cornelian cherry) is one of the fruit species that has attracted attention in recent years and is a widely used species from traditional consumption to modern industrial applications due to its high nutritional content, richness in bioactive compounds, and functional food potential. Türkiye is very rich in terms of dogwood genetic resources that can be grown from seed and used for different breeding purposes. This study aimed to characterize the variation in fruit/plant quality attributes and to assess the underlying genetic diversity among 30 C. mas L. genotypes using interprimer binding site (iPBS) markers. A comprehensive set of pomological traits (e.g., fruit size, weight, length) and biochemical parameters (soluble solids, titratable acidity, total phenolic content, and antioxidant capacity) was recorded at harvest. Overall, substantial variability was observed among the genotypes. Some accessions combined larger fruit size with higher soluble solids content and antioxidant capacity, whereas others produced smaller fruits but exhibited higher acidity or phenolic content, suggesting distinct potential uses for fresh consumption and processing. Genetic diversity was evaluated using 12 iPBS primers, which generated a total of 89 bands, of which 75.3% were polymorphic. The mean polymorphism information content value was 0.89, with values ranging from 0.77 to 0.99, revealing moderate-to-high genetic differentiation among genotypes. Cluster and multivariate analyses grouped the accessions into four major clusters, which were largely consistent with their pomological and biochemical profiles. Taken together, these results provide an integrated view of pomological, biochemical, and molecular variation in C. mas L. genotypes and identify promising genotypes that can be exploited in future breeding and conservation programs.

Metabolomics analysis revealed the chemical mechanism of powerful antioxidant activity of Coffea arabica at high-altitude regions

Phenolic compound determination through electrochemical methodologies and biosensors: A review supported by bibliometric analysis.

Supplementation of Baicalin (BC) in extender improves structural and functional characteristics, total antioxidant capacity, and in vivo fertility of ram semen.

Microvascular dysfunction (MVD) after ST-elevation myocardial infarction (STEMI) is common and contributes to adverse left ventricular remodeling and reduced survival. Endothelial glycocalyx (eGC) shedding during STEMI has been proposed as a potential mechanism for MVD. This study evaluated eGC shedding during STEMI, its recovery over time, and its correlations with oxidative stress, inflammation, and endothelial injury. In this before-after clinical study, STEMI patients were assessed in the acute phase and again six months later. Circulating biomarkers of eGC shedding (syndecan-1 [SDC-1], hyaluronan [HYAL], sulfated glycosaminoglycans [GAGs]); endothelial injury (thrombomodulin [TBML], tissue plasminogen activator [tPA], vascular endothelial growth factor [VEGF], nitrite); oxidative stress (malondialdehyde [MDA], total hydroperoxides [H₂O₂], superoxide dismutase [SOD], reduced glutathione [GSH], total antioxidant capacity [TAC]); and inflammatory mediators (IL-6, TNF-α) were quantified. Sublingual microcirculation was evaluated using GlycoCheck to estimate the perfused boundary region (PBR), an inverse parameter of eGC thickness. Twenty patients were included, with a mean interval of 188±31days between assessments. PBR and flow-adjusted PBR decreased significantly from the acute phase to follow-up, indicating eGC restoration. Acute elevations in SDC-1, HYAL, GAGs, MDA, H₂O₂, SOD, tPA, and nitrite were attenuated at six months. Biomarkers of eGC shedding and PBR values correlated positively with indices of oxidative stress, endothelial injury and inflammation. eGC shedding occurs during STEMI and shows evidence of recovery in the subsequent months. Correlations between eGC shedding and oxidative stress, endothelial injury and inflammation support a mechanistic interplay contributing to MVD development after STEMI.

This study investigated the effects of supplementation of dietary sesame seed (SS) on the performance, egg quality, serum biochemical profile, intestinal morphology, and microbial load of Rhode Island Red laying hens. A total of 240 Rhode Island Red hens (30 weeks old) were assigned to four dietary treatments in a completely randomized design for fifteen weeks, where sesame seed was incorporated at 0, 10, 15, or 20 g/kg in isonitrogenous diets. Performance parameters were recorded daily, egg quality was assessed biweekly, and blood, fecal, and intestinal samples were collected at the end of the trial for biochemical, microbiological, and histomorphological analyses. Partial substitution of soybean meal with sesame seed significantly (P < 0.05) improved daily and weekly egg production, hen-day egg production, and feed conversion ratio, with the highest replacement level (20 g/kg) yielding the greatest enhancement. Egg quality characteristics were not influenced by dietary treatment. The serum lipid profile improved significantly (P < 0.05), as sesame-based replacement reduced total and LDL cholesterol while increasing HDL cholesterol. Antioxidant status was also enhanced (P < 0.05), as indicated by increased SOD, GPx, and total antioxidant capacity, although MDA levels remained unaffected. Furthermore, soybean meal replacement with sesame seed reduced (P < 0.05) fecal Salmonella and Escherichia coli counts and improved (P < 0.05) duodenal morphology, reflected by shallower crypt depths and higher villus-to-crypt ratios. Overall, sesame seed up to 20 g/kg enhanced productivity, antioxidant defense, lipid metabolism and pathogen reduction without compromising egg quality, highlighting sesame seed as a viable alternative protein source in layer diets.

Green optimisation of microwave-assisted extraction of phenolic compounds from rice straw lignocellulosic residues and LC-HRMS-based cultivar profiling.

Fixation technology plays a crucial role in the processing of mulberry leaf green tea (MLGT); however, the effect of different fixation treatments on tea quality remains unexplored. The present study comprehensively investigates the antioxidant capacity, key non-volatile compounds (free amino acids, tea polyphenols, soluble sugars, total flavonoids, and alkaloids), and volatile profile of MLGT produced with four fixation techniques, namely hot-air roller, microwave, blanching, and steam. The key non-volatile compounds and antioxidant capacity (DPPH and FRAP evaluation systems) were highest in the microwave-processed MLGT sample. A total of 1025 volatile compounds were identified using headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry, and aldehydes were the most predominant aromatic compounds in MLGT. The total content of aromatic compounds (peak area) was highest in the hot-air roller processed MLGT sample. Notably, 4-(2,6,6-trimethyl-1-cyclohexen-1-yl)-3-buten-2-one, (E)-1-(2,6,6-trimethyl-1,3-cyclohexadien-1-yl)-2-buten-1-one, 5-(Z)-4-heptenal, (E,Z)-2,6-nonadienal, and 1-hepten-3-one were identified as key odor-active compounds (rOAV ≥ 100), which contributed floral, soybean, fatty, and green notes. These compounds were least abundant in the blanched MLGT sample. In addition, 481 differential volatiles were identified as contributing to the aromatic profile based on partial least squares discriminant analysis with variable importance in projection > 1 and P < 0.05. Flavor annotation from these differential compounds revealed that the microwave-processed MLGT sample contained the highest levels of desirable aromas (e.g., sweet, fruity, nutty, woody, and herbal), while the blanched tea sample had the lowest levels of unpleasant notes (e.g., green, spicy, and fatty). Microwave and blanching are recommended fixation methods for MLGT processing. This study provides a theoretical basis for optimizing fixation technologies to enhance the flavor quality and health-related properties of MLGT. © 2026 Society of Chemical Industry.

Evaluating poultry fat and crude palm oil blends on growth performance, fatty acid profile, lipid metabolism and antioxidant capacity in juvenile Asian seabass (Lates calcarifer)

Severe early-onset preeclampsia is significantly associated with metabolic dysregulation and sustained elevation of placental HIF-1α.

Comparative transcriptomics reveals distinct molecular responses to acid stress between conventional and hybrid rice

Deciphering the effects of plant oil diets in Channa striata (Bloch, 1793) using an integrated transcriptomic and metagenomic approach

Differential UV-B stress reconfigures antioxidant pathways and growth dynamics through coordinated transcriptional and enzymatic regulation in Raphanus sativus L

pH-responsive and self-adaptive hydrogel dressing based on a TA/PVA network co-loaded with cannabidiol and mupirocin for synergistic infected wound healing.

Bioactive molecules from Agave salmiana bagasse: Study of their antioxidant, prebiotic potential, and cytotoxicity.

A glucose-triggered self-reinforcing hydrogel based on carboxymethyl chitosan/oxidized hyaluronic acid/borax for delivering nano-selenium and dynamic diabetic wound management.

Development and characterization of functional cookies enriched with spray dried strawberry particles: Sensorial, textural, physicochemical properties, antioxidant capacity and bioaccesibility assay of phenolic compounds

Resin-mediated green synthesis of CuO nanoparticles from Boswellia papyrifera: Comprehensive characterization and potent antibacterial and antioxidant properties