Chilli fruit are good antioxidant sources and hold promise as natural ingredients in functional foods. Many other types of carotenoids accumulate in the fruit during the ripening process, for example, β-carotene, violaxanthin, lutein, and zeaxanthin. The present experiment was carried out to Effect of bio control agents and botanical extracts on antioxidant activity in chilli fruits. Chilli seedlings were treated with individually and in combination with effective bio control agents and botanical extracts in field condition applied as seedling treatment and foliar spray. Efficacy of botanical extract and bio control agents on antioxidant activity of fruits of chilli plant applied seedling treatment and foliar spray with T. Viride + P. Fluorescens + Neem + Garlic showed higher β-carotene and capsaicin content in chilli fruits in comparison to control. Maximum β-carotene (3.56 mg/mL) and capsaicin (2831.67µg/g) content were recorded in chilli fruits respectively. These antioxidants sources are responses to disease resistant against pathogen and benefiting the digestive tracts, promote healthy heart, relieve joint pains, promote weight loss, mitigate migraine, reduce cancer risk, prevent allergies etc.