Antibiotic Moxifloxacin Research Articles

Determination of the extent of protein binding of antibiotics by means of an automated continuous ultrafiltration method

The extent of the protein binding of a drug is an important pharmacokinetic parameter. In this study, the determination of the protein binding of the antibiotics gatifloxacin, moxifloxacin, linezolid and telithromycin to bovine serum albumin (BSA) and human serum albumin (HSA) was performed by means of an automated continuous ultrafiltration method. The continuous ultrafiltration has several advantages compared to classical methods like equilibrium dialysis or discontinuous ultrafiltration. The method is appropriate for a fast determination of the extent of the protein binding of a drug. In one single experiment the calculation of the protein binding over a wide range of different drug/protein ratios is possible. Comparing the results obtained with the continuous ultrafiltration with binding data reported in literature shows good correlation and proves the reliability of the method.

Hopkins scientist tunes in to treat tuberculosis

Hopkins scientist tunes in to treat tuberculosis

Effect of moxifloxacin on secretion of cytokines by human monocytes stimulated with lipopolysaccharide

Objective To determine the effect of moxifloxacin on secretion of cytokines by human monocytes stimulated with lipopolysaccharide (LPS) or Pansorbin.Methods Monocytes obtained from 10 healthy volunteer donors were stimulated with LPS or Pansorbin and exposed or not to different concentrations of the fluoroquinolone antibiotic moxifloxacin. At 3, 6 and 24 h, the amounts of interleukin-1α (IL-1α), IL-1β, IL-6, IL-10, IL-12 (p70) and tumour necrosis factor-α (TNF-α) were measured in the supernatants of the monocyte cultures using enzyme-linked immunosorbent assay.Results Stimulation of human monocytes with either LPS or Pansorbin resulted in a significant increase in secretion of each of the cytokines examined. Treatment of LPS-stimulated monocytes with moxifloxacin significantly inhibited (P<0.01) secretion of IL-1α by monocytes of each of 10 human donors; the secretion of TNF-α was significantly inhibited (P<0.01) in monocytes from six of 10 donors. In general there was a trend towards inhibition of secretion of IL-6, IL-10 and IL-12 (p70), but the inhibitory effect was not statistically significant. Secretion of cytokines by Pansorbin-stimulated monocytes was not significantly inhibited by moxifloxacin.Conclusions Moxifloxacin has immunomodulatory activity through its capacity to alter the secretion of IL-1α and TNF-α by human monocytes.

Spectrofluorimetric determination of moxifloxacin in tablets, human urine and serum.

A spectrofluorimetric method to determine the antibiotic moxifloxacin is proposed and was applied to pharmaceuticals, human urine and serum. The fluorimetric method allows the determination of 30-300 ng mL-1 moxifloxacin in aqueous solution containing phosphoric acid-phosphate buffer (pH 8.3) with lambda exc = 287 nm and lambda em = 465 nm. Detection and quantification limits were 10 and 30 ng mL-1, respectively, with a relative standard deviation (n = 10) of 2%. This method was applied to the determination of moxifloxacin in three Spanish commercial pharmaceutical formulations. Another variant of the method in micellar medium allows the direct measurement of moxifloxacin in human serum and urine by standard additions. The enhanced fluorescence of moxifloxacin in 8 mM sodium dodecyl sulfate (SDS) solution at pH 4.0 (acetic acid-acetate buffer) for lambda exc = 294 nm and lambda em = 503 nm shows the same linear range as the aqueous method with a 25% lower slope (with detection and quantification limits of 15 and 60 ng mL-1, respectively, and a relative standard deviation of 1.3%), but permits the background fluorescence for urine and serum blanks to be minimized. Hence, sufficient sensitivity is reached to determine therapeutic concentrations of the drug in urine (average recovery 102 +/- 2%) and serum (average recovery 105 +/- 2%) samples.