Antipsoriatic Activity Research Articles

Formulation and Evaluation of Nanogel used for the Treatment of Psorasis

Context: Although several formulation strategies have been developed for the treatment of psoriasis, there is an unmet need for optimization of its therapy. Objective: The objective was to develop a nanogel composed of Acitretin (ACT) loaded nanostructured lipid carrier (ACT-NLC) and to evaluate its potential in imiquimod-induced psoriasis model to ameliorate symptoms of psoriasis. Materials and methods: The developed control (CNGs) nanogels, acitretin loaded nanogels (ActCNGs) were characterized by UV and FTIR. Results and discussion: The systems were found to be spherical in shape with a size range of 98±10, and 138±8 nm having zeta potential values of +28±3 and +27±3 mV for CNGs and ActCNGs respectively. The in vitro haemolysis assay revealed that all the nanogel systems are blood compatible. The systems exhibited higher swelling and release at acidic pH. The ex vivo skin permeation studies using porcine skin confirmed the higher deposition of the systems at epidermal and dermal layers, which was confirmed further by fluorescent imaging. The in vivo anti-psoriatic activity study using Perry's mouse tail model and skin safety studies confirmed the potential benefit of the system for topical delivery of acitretin in psoriasis.

Assessment of anti-psoriatic activity of bakuchiol-loaded solid lipid nanoparticles-based gel: design, characterization, and mechanistic insight via NF-kB signaling pathway.

The aim of the current study is to evaluate the anti-psoriatic potential of bakuchiol (Bak) loaded solid lipid nanoparticles (SLNs) via modulating inflammatory and oxidative pathways. Bak-loaded SLNs were prepared using hot homogenization method and characterized by various spectroscopic techniques. Bak-SLNs suspension was formulated into gel using Carbopol. Different in vivo assays were executed to explore the role of inflammatory markers and oxidative enzymes in psoriasis. DLS (dynamic light scattering) analysis showed suitable particle size, zeta potential, and polydispersity index (PDI) of developed formulation. TEM (transmission electron microscopy) reveal the spherical shape of Bak-SLNs particles. The release studies confirmed the sustained release of Bak-SLNs-based gel. UV-B-induced psoriatic Wistar rat model showed significant anti-psoriatic effect of Bak via regulating inflammatory markers (NF-kB, IL-6, IL-4, and IL-10) and levels of anti-oxidant enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and glutathione-S-transferase (GST). Furthermore, RT-qPCR analysis confirms that Bak downregulates the expression of inflammatory markers, while histology and immunohistology results also confirm the anti-psoriatic effect of Bak. The study indicates that Bak-loaded SLNs-based gel significantly downregulates the level of cytokines and interleukins involve in NF-kB signaling cascade; hence, it can prove to be a novel therapeutic approach to cure psoriasis.

Effect and mechanism of longkui yinxiao soup in treating psoriasis in mice.

Objective: Longkui Yinxiao Soup is a traditional Chinese medicine formula used to treat psoriasis for decades. Although Longkui Yinxiao Soup showed promising efficacy in clinical practice, the regulatory mechanisms of Longkui Yinxiao Soup remain elusive. This study aimed to explore the underlying mechanisms of Longkui Yinxiao Soup in a psoriasis-like mouse model. Methods: Longkui Yinxiao Soup was quality controlled by determining the contents of imperatorin and rhoifolin using high-performance liquid chromatography. The imiquimod-induced psoriasis-like mouse model was used to study the therapeutic effect and mechanism of Longkui Yinxiao Soup. The histopathological skin changes were observed by hematoxylin and eosin staining; the infiltration of proliferating proteins, proliferating cell nuclear antigen and Ki67, in skin tissues were observed by immunohistochemical analysis; and the inflammatory factors such as interleukin (IL)-6, tumor necrosis factor (TNF)-α, IL-23, and IL-17 in serum were detected using enzyme-linked immunosorbent assay. RNA sequencing and bioinformatic analysis were used to predict the mechanism of LYS against psoriasis. mRNA expressions of p38, extracellular regulated protein kinases (ERK), mitogen-activated protein kinase 3 (MEK3), mitogen-activated protein kinase 6 (MEK6), RAP1 GTPase activating protein (Rap1gap), and Rap1 were determined using real-time quantitative polymerase chain reaction. The expression levels of proteins related to Rap1-mitogen-activated protein kinase signaling pathways were measured by Western blotting. Results: A quality-control method for Longkui Yinxiao Soup was successfully established using imperatorin and rhoifolin as content determination indexes. Longkui Yinxiao Soup significantly ameliorated the psoriatic symptoms in mice. The serum levels of inflammatory cytokines such as IL-6, TNF-α, IL-23, and IL-17 were decreased, and the expression levels of antigen identified by monoclonal antibody Ki67 (Ki67) and PCNA in skin tissues were downregulated. Moreover, the inhibition of Rap1-MAPK signaling pathways by Longkui Yinxiao Soup was detected. Conclusion: This study confirmed the antipsoriatic activity of Longkui Yinxiao Soup in psoriasis-like mice. This might be due to the inhibition of inflammatory factor secretion, keratinocyte proliferation, and the Rap1-MAPK signal pathway.

In vivo evaluation of the antipsoriatic effect of hydrogel with lavandin essential oil and its main components after topical application

In our study, lavandin oil was hydrodistillated from Lavandula × intermedia (Lamiaceae), and new alginate-based hydrogel formulations containing lavandin oil, linalool, and linalyl acetate were prepared for the first time. Using the gas chromatography (GC) and gas chromatography - mass spectrometry (GC-MS) methods fifty eight components of obtained essential oil were identified. Subsequently, the antipsoriatic activity of the created formulations was investigated by applying an imiquimod-induced mouse model. Alginate-based hydrogels were used as carriers for lavandin oil, linalool, and linalyl acetate. The histopathological examination of imiquimod-induced psoriasis-like mice ear skin stained with H&E (haematoxylin and eosin) was conducted after applying the examined formulations. Additionally, the impact of lavandin oil, linalool, and linalyl acetate on the expression of CD3 (cluster of differentiation 3), CD68 (monoclonal mouse anti-human), and Ki67 (marker of proliferation Ki-67) were studied. Histopathological studies showed that analysed formulations decreased the mice ears’ thickness and the analysed psoriasis symptoms (parakeratosis epidermal thickening, hypertrophy of the spinous layer, inflammatory infiltrates, Munro's microabscesses, Kogoj's micro-pustules, and dermal papillae oedema). The prepared formulations inhibited proliferation of the cells (Ki67 staining method) and expression of CD3 and CD68. The most potent activity against the inflammation in psoriasis was the preparation containing 5% lavandin oil.

TOPICAL DELIVERY OF PAPAIN FOR MANAGEMENT OF PSORIASIS: FORMULATION DEVELOPMENT AND ASSESSMENT OF ANTI-PROLIFERATIVE ACTIVITY USING HACAT CELL LINE

The study was aimed at developing topical gel containing papain as the drug and assessing its antipsoriatic activity. Carbopol was used as the polymer and drug-excipient compatibility was ascertained by infrared spectroscopy (Fourier transform) and differential scanning calorimetry. All prepared formulations were evaluated for viscosity, pH, homogeneity, extrudability, spreadability, in vitro diffusion and release kinetics. Anti-proliferative activity was determined on HaCaT cell line. The optimized formulation, F2 exhibited pH 6.8, optimum viscosity, extrudability and spreadability for convenient use. The optimized formulation followed Higuchi kinetic model with release mechanism being super-case two transport. The anti-proliferative activity of optimized formulation on HaCaT cell line showed only 3.9 % cells to be viable with respect to control confirming its efficacy in treating psoriasis. The gel exhibited stability under accelerated stability conditions. It can be inferred from the study that papain gel can be effectively used for managing psoriasis and its single application would give prolonged action.

Metabolomics analysis reveals cytotoxic effects of ouabain towards psoriatic keratinocytes via impairment of glutathione metabolism

Ouabain is a cardiac glycoside long studied for treating heart diseases, but the attempts to evaluate its anti-psoriatic activity have not been reported. We aimed to explore the effects of ouabain on proliferation and metabolism towards psoriatic keratinocytes. In human HaCaT keratinocytes, ouabain potently decreased viability, promoted apoptosis and caused G2/M cycle arrest. Metabolomics analysis indicated that ouabain markedly impaired glutathione metabolism. The solute carrier family 7 member 11 (SLC7A11) is an amino acid transporter highly specific to cysteine, which is critical for glutathione synthesis. Ouabain downregulated SLC7A11, reduced cysteine uptake and subsequently inhibited glutathione synthesis, probably through inhibiting Akt/mTOR/beclin axis that regulate protein activity of SLC7A11. The impaired glutathione synthesis and oxidative stress caused by ouabain may contribute to its cytotoxicity towards psoriatic keratinocytes. Our results provide experimental evidence supporting further study of ouabain as a potential anti-psoriatic agent.

Optimization of the Transdermal Delivery System in Astilbin Microemulsion with Improved Stability and Anti-psoriatic Activity.

Astilbin is a promising candidate drug for psoriasis. However, the poor solubility and stability limited its clinical application. The present work aimed to develop a stable microemulsion of astilbin formulation and evaluate its effect in vitro and in vivo. Oil phase, surfactants, and cosurfactants were screened using solubility and stability of astilbin as the index. The central composite experiment design and response surface methodology analysis were adopted to optimize microemulsion parameters. The particle size, zeta potential, polydispersity index, viscosity, drug content, encapsulation, transmission electron microscopy (TEM), and stability of the optimized microemulsion were evaluated. Then, the drug release and anti-psoriasis effects were evaluated in a mouse model induced by imiquimod. The optimum formulation contained Labrafil M 1944 Cs (10.12%), Polyoxyethylene Castor Oil 35 (37.41%), propylene glycol (12.47%), water (40%), and gallic acid (2.9%), and the average particle size was 14.71 nm. The permeability of astilbin from the optimized astilbin-gallic acid microemulsion in 24 hr was 4.39 times higher compared with the astilbin's microemulsion. The content of astilbin in astilbin-gallic acid microemulsion remained unchanged after being stored at 25°C for 4 months compared with astilbin aqueous (3 h) and astilbin microemulsion (185 h). Compared with the model group, the optimized formulation decreased the PASI score and Baker score by 49% and 73%, respectively, which showed a favorable anti-psoriasis effect. Moreover, there was no difference in the anti-psoriasis effect between the optimized group and the positive control. These results indicated that the astilbin-gallic acid microemulsion might be a potential topical drug used for the treatment of psoriasis.

Synthesis, Characterization and Pharmacological Evaluation of Ethanolic Extract of Sarcostemma brevistigma for its Anti-inflammatory and Antipsoriatic Activity

The goal of this work was to test the anti-inflammatory and anti-psoriatic properties of an ethanolic extract of Sarcostemma brevistigma stem in the formation of a hydrogel utilizing several mouse models. The methanolic fraction was obtained by fractionating an ethanolic extract of Sarcostemma brevistigma stem with methanol. Viscosity, spreadability, drug release profile, pH, homogeneity, physical properties and irritant impact of the prepared hydrogel were assessed. Percentage inhibition, nitric oxide (NO), and myeloperoxidase (MPO) levels in mice ears following acute inflammation caused by croton oil were all measured. Physical examination revealed that the produced hydrogels were brownish in color and had a homogenous and smooth look when applied. All other test criteria, including as pH, viscosity, spreadability, and consistency, were determined to be acceptable in the F3 hydrogel formulation. The optimal F3 formulation component was 1.5 g of Carbopol 940 and 1% sodium CMC. In xylene-induced ear edema in mice, the percent suppression of edema was found to be equivalent to the standard group of therapy (69.58 %). The results of this investigation show that a produced hydrogel of SB extract has inhibitory activity on acute inflammation. The capacity of croton oil to promote neutrophil influx in mouse ear tissue was shown in this study. Treatment with extract hydrogels, as well as Voltaren Emulgel, substantially decreased MPO and NO levels in mice ears (P < 0.01). The presence of flavonoids in Sarcostemma brevistigma may explain why the methanol extracts have a substantial anti-inflammatory effect.

Discovery of orally bioavailable inhibitors of MALT1 with in vivo activity for psoriasis

We report the design, synthesis, and biological activity of a series of compounds that exhibit potent mucosa-associated lymphoid tissue lymphoma translocation 1 (MALT1) inhibition. Structural transformation of the substructures of a starting compound gave amidomethyl derivatives and sulfonylguanidine derivatives that exhibited potent inhibition of MALT1. Compound 37 had good oral bioavailability and showed anti-psoriatic activity in an imiquimod-induced psoriasis mouse model after oral administration.

Novel Synergistic Approach: Tazarotene-Calcipotriol-Loaded-PVA/PVP-Nanofibers Incorporated in Hydrogel Film for Management and Treatment of Psoriasis.

Psoriasis is an autoimmune skin disease that generally affects 1%-3% of the total population globally. Effective treatment of psoriasis is limited because of numerous factors, such as ineffective drug delivery and efficacy following conventional pharmaceutical treatments. Nanofibers are widely being used as nanocarriers for effective treatment because of their multifunctional and distinctive properties, including a greater surface area, higher volume ratio, increased elasticity and improved stiffness and resistance to traction, favorable biodegradability, high permeability, and sufficient oxygen supply, which help maintain the moisture content of the skin and improve the bioavailability of the drugs. Similar to the extracellular matrix, nanofibers have a regeneration capacity, promoting cell growth, adhesion, and proliferation, and also have a more controlled release pattern compared with that of other conventional therapies at the psoriatic site. To ensure improved drug targeting and better antipsoriatic efficacy, this study formulated and evaluated a tazarotene (TZT)-calcipotriol (CPT)-loaded nanofiber and carbopol-based hydrogel film. The nanofiber was prepared using electrospinning with a polyvinyl alcohol/polyvinylpyrrolidone (PVA/PVP) K-90 polymeric blend that was later incorporated into a carbopol base to form hydrogel films. The prepared nanofibers were biochemically evaluated and in vitro and in vivo characterized. The mean diameters of the optimized formulation, i.e., TZT-loaded polyvinyl alcohol/polyvinylpyrrolidone nanofiber (TZT-PVA/PVP-NF) and TZT-CPT-loaded polyvinyl alcohol/polyvinylpyrrolidone nanofiber (TZT-CPT-PVA/PVP-NF) were 244.67 ± 58.11 and 252.31 ± 35.50 nm, respectively, as determined by scanning electron microscopy, and their tensile strength ranged from 14.02 ± 0.54 to 22.50 ± 0.03 MPa. X-ray diffraction revealed an increase in the amorphous nature of the nanofibers. The biodegradability studies of prepared nanofiber formulations, irrespective of their composition, showed that these completely biodegraded within 2 weeks of their application. The TZT-CPT-PVA/PVP-NF nanofibers exhibited 95.68% ± 0.03% drug release at the end of 72 h, indicating a controlled release pattern and following Higuchi release kinetics as a best-fit model. MTT assay, antioxidant and lipid profile tests, splenomegaly assessment, and weight fluctuation were all performed in the in vitro as well as in vivo studies. We found that the TZT-CPT-PVA/PVP-NF-based hydrogel film has high potential for antipsoriatic activity in imiquimod-induced Wistar rats in comparison with that of TT-PVA/PVP-NF nanofibers.

ANTIPSORIATIC ACTIVITY OF HYDROGEL CONTAINING NANOSTRUCTURED LIPID CARRIER (NLC) ENTRAPPED WITH TRIAMCINOLONE ACETONIDE

Objective: The aim of present study was to prepare triamcinolone acetonide (TA) loaded NLCs hydrogel for antipsoriatic activity. Methods: A Nanostructured lipid carrier (NLCs) was prepared by using solvent diffusion and high pressure homogenization methods. NLCs dispersion was characterized by particle size, zeta potential, scanning electron microscopy (SEM), differential scanning calorimetry, and an in vitro release study. Optimized NLC incorporated into the hydrogel and characterized for rheological properties, drug content, in vitro drug release, stability study, skin irritation and antipsoriatic activity for optimized batch of hydrogel. Results: Optimized NLCs loaded with TA were exhibited spherical shape with particle size 286±0.07 nm, polydispersity index 0.317, zeta potential-21.91±0.05mV and entrapment efficiency 86.19±0.06% respectively. The drug release of optimized batch was 8.34 % and 88.84±0.08% at 1h and 8h respectively. The release kinetics of the optimized NLCs best fitted the peppas-korsmeyer model. The results of NLC hydrogel formulations were spreadability 27.4±0.06-11.76±0.07 g. cm2/sec, drug content 65.60±0.05%-74.50±0.02%, in vitro drug release 87.52±0.04%, primary irritation index was 0.0752, it indicates barely perceptible irritation. Histopathological studies showed that, in psoriasis-induced animal treated with TA loaded NLC hydrogel, marked reduction in thickness of epidermis, as compared to conventional gel formulation. It shows the increase % orthokeratosis 88.69% and % drug activity 54.23% than the marketed formulation. Conclusion: The present results demonstrated that hydrogel based NLC shows the better and effective drug delivery for the management of psoriasis.

Development and evaluation emulgel for effective management of the imiquimod-induced psoriasis.

Our main aim is in the present investigation, development and evaluation of seabuckthorn oil-based Emulgel formulation for psoriasis therapy. Anti-psoriatic activity of the SeaEmulgel was studied using Imiquimod-induced psoriasis-like inflammation model Balb/c mice and parameters such as PASI score, ear thickness, spleen to body weight index including histological staining studies, enzyme-linked immune sorbent assay (ELISA), skin compliance and safety evaluation of sea buckthorn oil was performed. The globule size and PDI of sea buckthorn oil emulsion were found to be 172.70 ± 1.73nm and 0.117 ± 0.018, respectively. In-vivo animal studies performed on male Balb/c mice and emulgel showed a reduction in redness, scaling, inflammation in psoriasis-induced mice, which was analysed by PASI scoring, body weight, spleen weight index and ear thickness. The current investigation clearly revealed the better anti-psoriatic activity of SeaEmulgel formulation against imiquimod-induced psoriasis-like skin inflammation Balb/c mice model.

Phytochemical evaluation and anti-psoriatic activity of the ethanolic extract of the leaves of Thespesia populnea

Psoriasis is a chronic, mild and common inflammatory skin condition. Still an ideal treatment for psoriasis, effective, safe, convenient, and economical is not available. In this scenario, the search for suitable alternative treatments with minimal side effects is necessary. Plants can be effective and alternative in this regard. Therefore, this article discusses the leaves of the plants Thespesia populnea (Malvaceae) that are traditionally used in the treatment of psoriasis. The present study aimed to assess anti-psoriatic activity. The dried leaves of the plants were subjected to soxhlation with 95% ethanol and phytochemical studies were performed. The anti-psoriatic activity was evaluated by the Mouse-Tail model. It is a relatively sensitive and reproducible morphometric method that allows quantitative evaluation of the effects of anti-psoriatics through epidermal differentiation. Extracts were applied topically at a dose of 500mg/kg over 14 days and at the end, the animals were sacrificed, longitudinal histological sections were made of the tail skin and the degree of orthokeratosis was determined. It was significantly (P <0.05) increased by the ethanolic extract of Thespesia populnea (52.86±2.86) compared to the control (17.30±4.09). In relative epidermal thickness, the ethanolic extract of Thespesia populnea (92.68±8.8) showed a significant difference (P <0.05) compared to the control (100±10.7). The data obtained suggest that the selected plant has anti-psoriatic activity and confirms its traditional use in the treatment of psoriasis.

Evaluation of the Anti-Psoriatic Activity of Dapagliflozin and Levofloxacin Ointment on the Tail Mice

Evaluation of the Anti-Psoriatic Activity of Dapagliflozin and Levofloxacin Ointment on the Tail Mice

Thymoquinone-Loaded Essential Oil–Based Emulgel as an Armament for Anti-psoriatic Activity

Essential oils consist of oxygenated structures of secondary metabolites of aromatic plants with anti-psoriatic activities. Tea tree oil (TTO) is an essential oil with good anti-microbial and anti-inflammatory properties, exhibiting reduced levels of IL-1, IL-8, and PGE 2. Thymoquinone (TMQ) is popular herb in traditional medicine with known therapeutic benefits in several diseases and ailments. The ternary phase diagram was prepared with the weight ratio of Smix (Tween® 80:Labrasol®): oil:water ratio for o/w emulsion preparation. The globule size was 16.54 ± 0.13nm, and PDI around 0.22 ± 0.01 of the TTO-TMQ emulsion and found thermodynamically stable. The percentage drug content was found in the range of 98.97 ± 0.62 to 99.45 ± 0.17% with uniformity of the ThymoGel using Carbopol®. The extensive physicochemical properties were studied using different analytical techniques, and in vitro drug release was performed using Franz-diffusion apparatus. Anti-psoriatic activity of the formulations was studied using Imiquimod-induced psoriasis-like inflammation model in male Balb/c mice and parameters like PASI score, ear thickness, and spleen to body weight index were determined as well as histological staining, ELISA, skin compliance, and safety evaluation of TTO were performed. The combination of essential oils with TMQ shows synergistic activity and efficiently reduces the psoriasis disease condition.

Mechanisms and Effects of Isorhamnetin on Imiquimod-Induced Psoriasiform Dermatitis in Mice.

Isorhamnetin (IRh), which has a wide range of pharmacological effects, is one of the most significant active components in the fruits of Hippophae rhamnoides L. and the leaves of Ginkgo biloba L. It protects the heart and brain, in addition to possessing anti-tumor, anti-inflammatory, antioxidant, organ protection, and anti-obesity properties. We sought to assess IRh's anti-psoriatic activity, explore its immunomodulatory properties in reducing the severity of psoriatic symptoms, and evaluate its potential immunotherapeutic effects. We used IRh to treat imiquimod (IMQ)-induced psoriasis in BALB/C mice and examined the underlying mechanisms. The outcomes demonstrated that IRh reduced epidermal hyperplasia, lowered PASI scores, and improved histopathological psoriasiform lesions in IMQ-induced mice. IRh attenuated the accumulation of malondialdehyde (MDA), and also reversed the reduction caused by IMQ of superoxide dismutase (SOD) and catalase (CAT) in skin tissues. Additionally, IRh effectively inhibited IMQ's ability to increase proinflammatory cytokines such as TNF-α, IL-6, IL-17A, and transcription factor NF-κB. Furthermore, IRh significantly reduced the percentage of Th1 and Th17 in the spleens of mice treated with IMQ and suppressed the maturation of splenic dendritic cells. Overall, our research suggests that IRh protects against oxidative stress and inflammation in the pathogenesis of psoriasis, with potential for the development of new and potent medication for the treatment of psoriasis.

A new perspective for psoriasis: Dithranol nanosponge loaded hydrogels

We developed dithranol nanosponge integrated Carbopol hydrogel (DTHNS-HG) as a novel perspective for psoriasis management. Dithranol was chosen as antipsoriatic moiety for this system. In this study, our objective was to fabricate and evaluate DTHNS-HG for in vivo anti-psoriatic activity using mouse tail model. Nanosponges (NSs) were synthesized using diphenyl carbonate and β-cyclodextrin employing melt method and appropriately characterized. The selected nanosponges were then embedded into Carbopol hydrogels and assessed for viscosity, spreadability and texture analysis. The developed formulations were further evaluated in vivo for antipsoriatic potential (% drug activity,% orthokeratosis and relative changes in epidermal thickness). Additionally, oxidative stress markers were also checked in mouse tail samples. DTH loaded cyclodextrin nanosponges were successfully fabricated and displayed acceptable mean particle size (274.6 ± 43.54 nm with 0.545 PDI) and zeta potential (-28.3 ± 6.34 mV). Further, DTHNS amalgamated hydrogel showed satisfactory results in terms of spreadability, viscosity and texture profiles. The degree of orthokeratosis was significantly (p < 0.05) increased with DTHNS-HG (0.5 and 1.0% w/v) and marketed ointment, as compared to the untreated group (control). Similarly, the drug activity and changes in epidermal thickness were found significant. Our findings from present investigations suggested a new cargo for delivery of DTH in topical medicinal strategies for psoriasis. Hence, this novel hydrogel showed promising outcomes for psoriasis.

Pharmacologically Active Secondary Metabolites from Psoralea corylifolia

Psoralea corylifolia has gained much attention, particularly in the cosmetic industry for the past few years owing to promising pharmacological activities of its metabolites. Seeds of P. corylifolia are the main source of bakuchiol, a meroterpene compound that is extensively harnessed in numerous skincare products. Furanocoumarins, psoralen and isopsoralen are other metabolites mainly from P. corylifolia seeds and known for their antipsoriatic activity. Moreover, various studies have reported several classes of secondary metabolites from this plant possessing diverse biological activities. This article highlights recent updates on P. corylifolia phytoconstituents and their promising pharmacological activities based on scientific publications during the last 10 years (2011-2021). The literature search was carried out through scientific-based websites and databases such as Google Scholar, NCBI, and PubMed. This paper included sixty-three bioactive metabolites reported in the last 10 years, belonging to the group of flavonoids, meroterpenes, furanocoumarins, coumestans, steroid and phenolic compounds. These phytoconstituents displayed a broad range of bioactivities including anti-inflammatory, antibacterial, antidiabetic, controlling obesity, hepatoprotective and cytotoxicity.&#x0D; Keywords: Bakuchiol, pharmacological activities, Psoralea corylifolia, bioactive metabolites.

In-silico Study of Phytoconstituents from Tribulus terrestris as potential Anti-psoriatic agent

Introduction: Psoriasis (Ps) is a well-known chronic non-infectious, inflammatory skin disease affecting about 2–3% of the worldwide Population. Pathogenesis includes the environmental trigger factors with other factors like genetic factors, trauma, chemicals, bacterial infection etc. Currently there is no drug which can permanently cure the skin lesions as well as completely eradicate this dermatosis. The purpose of this research is to investigate the anti-psoriatic activity of phytoconstituents of Tribulus terrestris by predicting the ligand-receptor binding and by predicting the ADMET parameters using Lipinski's rule. Methodology: The process of research work starts with protein and ligand structure preparation. Further docking was done using PyRxAutodock Vina. Afterward, analysis and visualisation of the interaction between protein-ligands was done, and ADMET profiling was carried out according to lipinski's rules using Swiss ADME. Result: we selected four phytoconstituents of Tribulus terrestris. Molecular docking simulation showed all four compounds had better binding affinities. Based on the results of prediction of ADMET values using the Lipinski rule, compound that are thought to have good activity. Conclusion: Based on results these molecules have discovered that they may be able to produce anti-psoriatic activity and found that they have a lower toxicity, and ADME analysis determined the easily absorbability to the tissue site. Hence, these compounds can be analysed by further in vitro studies and can be a leader in the designing of the potential drug for the psoriasis management.

TOPICAL DELIVERY OF EXTRACTIVE PHYTOCONSTITUENTS OF SOYABEAN SEEDS FOR MANAGEMENT OF PSORIASIS

The aim of the present study was to evaluate antipsoriatic activity of extractive phytoconstituents of the seeds of Glycine max (soyabean). Oxidative stress is considered as one of the important etiopathological factors in development and exacerbation of psoriasis. Isoflavones, the most important phytoestrogens present in soyabean, possess anti-inflammatory and antioxidant properties which make them vital phytoconstituents for management of psoriasis. The prepared extracts of soyabean seeds were evaluated for physicochemical properties and presence of phytoconstituents. On the basis of phytochemical screening and total phenolic content (TPC) of various extracts prepared, ethanolic extract (EE-S), hydroalcoholic extract (HAE), water extract (WE) and petroleum ether extract (PEE) were selected for further evaluation of antioxidant efficacy. Ethanolic extract of soyabean (EE-S) showed highest free radical scavenging activity and hence further formulated as conventional gel. The developed formulation was evaluated for physicochemical characteristics such as spreadability, in vitro release study and in vivo study for determination of efficacy in reducing psoriatic lesions in Wistar rats using photodermatitis model.